KR100866985B1 - Device and method for cultivating microorganism - Google Patents

Abstract

An apparatus of culturing microorganism and a method using the same is provided to improve productivity by circulating and culturing automatically a culture fluid agitated from outside, occur no break down of microorganism by a high temperature by heating the culture fluid gradually by heat-exchange, improve marketability and culturability, to sterilize and wash bacteria easily by circulating high temperature of steam and to maximize effectiveness of the microorganism propagation by minimizing molecule of the water when microbe is cultivated. An incubation room is formed inside a culture tank(100). A culture medium multiplies by circulating water and culture fluid. The culture medium is comprised of a connection pipe(200), a water pipe(300), a pump(400), a heating portion(500) and a culture fluid feed port(600). The water pipe supplies the water to the incubation room through the connection pipe. The pump circulates the water stored in the incubation room through the connection pipe. The heating portion heats the water passing through the connection pipe. The culture fluid feed port supplies the culture fluid to the incubation room through the connection pipe. The upper heating part comprises a steam boiler(510), the first heat exchanger(520) and the second heat exchanger(530). The first heat exchanger is connected to the steam boiler. One side of the second heat exchanger is connected to the connection pipe. The other side of the second heat exchanger is connected to the first heat exchanger. The steam boiler is connected to the upper side of the culture tank through the steam supply pipe. The steam boiler supplies the steam inside the incubation room.

Description

Microbial culture apparatus and method {DEVICE AND METHOD FOR CULTIVATING MICROORGANISM}

The present invention relates to a microbial culture apparatus and method, and more particularly, to a microbial culture apparatus and method for improving productivity by automatically circulating the culture culture stirred outside.

In general, microorganisms are widely used in agriculture, animal husbandry, fisheries, etc. As a method of using these microorganisms, photosynthetic bacteria, lactic acid bacteria, Bacillus bacteria, yeast bacteria, cyanobacteria, etc. are cultured and diluted in water, followed by disinfection of seeds and soil. The crops are then fed to irrigation or foliar sprays and livestock.

These microorganisms promote crop production, prevent pests, promote growth in livestock, prevent disease, improve meat quality, reduce odor and contamination of feces, and also inhibit the growth of pathogens or beneficial bacteria. It is used to help to get rid of harmful bacteria.

In addition, microorganisms can be used in the field of water purification, organic waste fermentation agent, various industrial waste purification, and when used in aquaculture, water purification, suppressing the occurrence of pathogens, improving disease resistance.

Since these microorganisms are cultured by using a constant nutrient, temperature, and light in some cases, conventional microbial incubators require a long time management using a large number of exothermic lamps to satisfy a light source, and at this time, microorganisms are cultured for a long time. As seen from the above, the light source is satisfied by the irradiation lamp such as a heating lamp, but the temperature keeps rising so that the control to the proper temperature is not smooth.

An example for solving such a problem is disclosed in Document 1 below.

As shown in FIG. 1, the technique disclosed in Document 1 includes a body 10, a culture tank 50 made of a light-transmissive material, which is located inside the body 10 and has a culture chamber 51 formed therein, and An inner cover (not shown) positioned inside the body 10 and covering the upper portion of the culture tank 50, and an irradiation lamp installed on an inner wall of the body 10 to supply light and heat necessary for culturing microorganisms ( 40 and an infrared ray blocking cover 60 for blocking infrared rays emitted from the irradiation lamp 40.

That is, by installing an infrared ray shield cover 60 to block the infrared ray in the irradiation lamp 40, the light source for the microorganism is sufficiently supplied, and excessive temperature rise is prevented, so that stable temperature control of the microorganism incubator is possible.

[Document 1] Patent Registration Publication No. 10-0732193 (June 19, 2007)

However, the microbial incubator disclosed in Document 1 is larger than the user's height as the culture volume increases. At this time, in order to inject the culture medium (medium) into the culture chamber, the user inputs the culture solution using a ladder and a hoist for raising the culture solution, there was a problem in use inconvenience.

In addition, since the user is transferred to the culture tank after stirring the culture solution from the outside, there is a problem that the stirring and culturing process must be performed separately.

In addition, as the irradiation lamp is installed inside the body, the microorganisms close to the irradiation lamp are destroyed by high temperature, thereby reducing the proliferation of microorganisms and greatly reducing the merchandise.

In addition, the microbial incubator is often required to sterilize and wash the inside of the culture chamber, but the microbial incubator of Document 1 has a problem that sterilization and washing are not easy because the user needs to open the cover directly.

The present invention has been made to solve the above problems, it is an object of the present invention to provide a microbial culture apparatus and method for improving the productivity by circulating the culture cultured from the outside automatically.

In addition, another object of the present invention is to provide a microbial culture apparatus and method which can greatly improve the commerciality and cultureability since the culture medium is gradually heated by heat exchange so that microbial destruction is not caused by high temperature.

In addition, another object of the present invention to provide a microbial culture apparatus and method that is easy to sterilize and wash by circulating high temperature steam.

In addition, another object of the present invention is to provide a microorganism culturing apparatus and method for improving the cultureability by maintaining the appropriate temperature of the culture medium using indirect heating by direct heating and heat exchange with steam required for microbial culture.

In addition, another object of the present invention to provide a microbial culture apparatus and method configured to discharge the gas generated during microbial culture.

In addition, another object of the present invention to provide a microbial culture apparatus and method for maximizing the efficiency of microbial growth by minimizing the molecules of water in microbial culture.

Microbial culture apparatus of the present invention for achieving the above object, the culture apparatus for supplying water and culture medium to one or more culture tanks, the culture chamber to form a culture chamber therein and circulating the supplied water and culture medium Includes, the incubator, the connecting pipe is installed on both sides of the culture tank upper and lower surfaces connected to each other, the water supply pipe for supplying water to the culture chamber through the connection pipe, and the water stored in the culture chamber It characterized in that it comprises a pump for circulating through, a heating unit for heating the water passing through the connecting tube, and a culture medium supply unit for supplying the culture solution into the culture chamber through the connecting tube.

Here, the heating unit, the first heat exchanger is connected to the steam boiler, the steam boiler and the heat exchanger, one side is connected to the connection pipe and the heat exchanger and the other side is capable of heat exchange with the first heat exchanger It is preferably provided with a second heat exchanger connected, wherein the steam boiler is connected through the steam supply pipe and the upper surface of the culture tank to supply steam into the culture chamber.

In addition, it is preferred that a refrigerator is provided between the first and second heat exchangers to cool the heat source circulating therebetween.

And, it is preferable to install a heating tube for heating the culture in the culture chamber of the culture tank.

In addition, the lower surface of the culture tank is preferably connected to the air supply for supplying air to the culture chamber.

In addition, it is preferable that a halogen lamp for generating light is provided in the viewing window formed on the outside of the culture tank.

On the other hand, the culture tank is preferably provided with a heating tube.

In addition, the culture tank is preferably further provided with a spray nozzle for injecting the culture solution passed through the connecting pipe to the culture chamber.

In addition, the culture tank is preferably provided with a gas removal unit for discharging the gas generated in the culture chamber.

And, the discharge pipe for discharging the culture medium passing through the connection pipe to the outside on the connection pipe is connected, it is preferable that a plurality of reservoirs for storing the solution passing through the discharge pipe is installed on the discharge pipe.

Referring to the culturing method of the microbial culture apparatus having such a configuration, the pumping force of the pump installed in the connecting pipe after the step S10, the step S10 of supplying and storing water to the culture chamber of the culture tank through the water supply pipe and the connection pipe. In step S20 to circulate the water stored in the culture chamber through the connecting pipe, the steam boiler during the step S20 is performed to the water passing through the connecting pipe based on the heat source transferred through the first heat exchanger and the second heat exchanger. When the water of the culture chamber rises to a set temperature according to the step S30 of heating and the step S30, during the step S40 of supplying the culture solution stored in the culture solution supply unit installed in the connecting tube to the culture chamber through the connecting tube, the step S40 is performed. Depending on the type of culture medium, air or light is supplied to the culture room through the air supply unit or halogen to S50 step of culturing the microorganism, wherein S10 ~ S50 step is characterized by culturing the microorganism by the microorganism culturing apparatus of any one of claims 1 to 10.

Here, the step S30 further includes the step of generating heat by supplying electricity to the heating tube in order to improve the heating rate of the water stored in the culture chamber, when the culture of the microorganism is completed after the step, stop the operation of the steam boiler Simultaneously with the operation of the refrigerator to cool the heat source of the first and second heat exchanger, and based on the heat source of the cooled second heat exchanger cooling the culture medium passing through the connection pipe to further control the microbial culture rate; It is preferable.

In addition, if the culture rate of the microorganism is controlled after performing the step S60, it is preferable to further include a step S70 for supplying and storing the culture solution in a reservoir connected to the connecting tube.

Then, when the culture of the microorganisms is completed after performing the step S70, it is preferable to further include a step S80 for circulating the sterilized and washed by the high temperature steam of the steam boiler.

According to the microorganism culturing apparatus and method according to the present invention, the culture medium stirred in the external incubator is automatically circulated to the culture tank through a connection tube, thereby providing an effect of improving productivity.

In addition, since the culture medium is gradually heated and maintained by heat exchange, it provides an effect that destruction of microorganisms due to high temperature does not occur.

In addition, the hot steam is circulated through the steam supply pipe to the culture tank and the connection pipe to provide an easy sterilization and cleaning effect.

In addition, the heat required for culturing the microorganism provides an effect of improving the cultureability by maintaining the appropriate temperature by using a direct heating through the heating tube and indirect heating by heat exchange of the culture medium with the hot steam generated in the steam boiler.

In addition, the culture tank is provided with a gas removal unit provides an effect of discharging the gas generated during microbial culture.

In addition, it provides the effect of spraying the culture solution passed through the connecting tube evenly through the injection nozzle to the culture chamber.

In addition, there is an effect that can greatly extend the retention period of the microorganism by the freezer.

In addition, it provides the effect of maximizing the efficiency of microbial growth by minimizing the molecules of water by the monomolecular machine.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Figure 2 is a view showing a microbial culture apparatus according to the present invention, Figure 3 is a flow chart showing a microbial culture method according to the present invention.

As shown in FIG. 2, the microbial culture apparatus according to the present invention includes a culture tank 100 and a culture apparatus 190 for supplying water and a culture solution to the culture tank to multiply the culture solution.

And, the incubator 190 is connected to the upper and lower surfaces of the culture tank 100, the both ends are connected to the connection pipe 200, the water supply pipe 300 for supplying water to the culture tank 100, and Pump 400 for circulating the water stored in the culture tank 100 through the connecting pipe 200, the heating unit 500 for heating the water passing through the connecting pipe 200, and the culture tank ( 100) a culture medium supply unit 600 for supplying a microbial culture solution therein, an air supply unit 700 for supplying air into the culture tank 100, and a halogen lamp for generating light in the culture tank 100; 800.

The culture tank 100 is made of a double structure to improve the thermal insulation, there is formed a culture chamber 110 for culturing microorganisms, the lower portion of the plurality of legs for supporting the culture tank 100 It is provided at equal intervals, one side of the outer surface is provided with a gauge 120 for identifying the amount of culture solution stored in the culture chamber 100, the other side of the fermentation and culture state of the culture solution stored in the culture chamber 100 Is provided with a detector 130 for confirming, it is preferable that the detector 130 uses a PH detector.

In addition, a transparent or semi-transparent see-through window 140 is formed on the front of the culture tank 100, a halogen lamp 800 to be described later is provided on the outside of the see-through window 140.

In addition, the culture tank 100 is further provided with a spray nozzle 101 for injecting the culture solution passed through the connecting pipe 200 to the culture chamber 110, the injection nozzle 101 is the culture chamber 110 Evenly spray the culture solution to the inside of.

In addition, the culture chamber 110 of the culture tank 100 is a gas is generated when the culture of the microorganism, in order to discharge the culture tank 100 is provided with a gas removal unit 102, the gas removal unit 102 ) Is composed of a valve that is opened and closed by a signal. Since such a valve is known, a detailed description thereof will be omitted.

On the other hand, the gas removal unit 102 performs the opening and closing operation manually and automatically by the pressure gauge 103 or the pressure sensor 104 provided in the culture tank (100).

The incubator 190 is installed on the outside of the culture tank 100 to supply water and the culture solution to the culture chamber 110, and to circulate and propagate the supplied water and the culture solution.

That is, the incubator 190 includes a connection pipe 200, a water supply pipe 300, a pump 400, a heating part 500, a culture solution supply part 600, an air supply part 700, and a halogen lamp 800. It will be described in more detail below.

The connection pipe 200 is for circulating the culture solution stored in the culture chamber 110 of the culture tank 100, the front end is connected to the upper surface of the culture tank 100, the lower end of the culture tank ( It is connected to the lower surface of the 100).

In addition, valves 210 and 220 for selectively supplying and blocking the culture solution stored in the culture chamber 110 are respectively installed at the front and lower ends of the connection pipe 200, and the upper one side is provided for supplying water. The water supply pipe 300 is connected and installed, and the pump 400 is installed at one lower side.

The water supply pipe 300 is for supplying water into the culture chamber 110, one end is connected to the water supply line provided on the outside, the other end is connected to the upper one side of the connecting pipe 200 is installed, the line One side is provided with a valve 310 for supplying or blocking water, and the other side of the line is provided with a single molecule machine 320, the single molecule machine 320 is a molecule of water passing through the water supply pipe 300 Minimized to maximize the efficiency of microbial growth.

In addition, the water supply pipe 300 is provided with a filter to remove foreign substances contained in the incoming water.

The pump 400 is for forcibly circulating the culture solution stored in the culture chamber 110 through the connection pipe 200, and is connected to the center of the connection pipe 200. On the other hand, the installation position of the pump 400 need not be limited to the center as long as it is a position where the culture medium can be forced through the connecting pipe 200, it can be installed in any position.

That is, the pump 400 provides a pumping force in the connection pipe 200 to the culture medium 110 through the culture chamber 110, the lower portion of the connection pipe 200, the upper connection pipe 200 through the culture chamber 110. Forced to circulate.

The heating unit 500 is for heating the culture medium circulating through the connection pipe 200 through the pump 400 to a set temperature and sterilizing and cleaning the microbial culture apparatus of the present invention, the steam boiler 510 to generate steam ), A first heat exchanger 520 that is capable of heat exchange with the steam boiler 510, and one side thereof is connected to the connection pipe 200 so that heat exchange is possible, and the other side of the first heat exchanger 520. ) And a second heat exchanger 530 that is capable of heat exchange.

Here, the first and second heat exchangers 520 and 530 have the same configuration as a heat exchanger that is commonly used, and thus detailed description thereof will be omitted.

In addition, only one of the first and second heat exchangers 520 and 530 may be installed.

That is, the heat source generated when the steam boiler 510 is operated is transferred to the first heat exchanger 520, the heat source of the first heat exchanger 520 is transferred to the second heat exchanger 530, and the second heat exchanger 530 is provided. Based on the heat source of the heat exchanger 530 is to heat the culture fluid passing through the connection pipe (200).

The water supply pipes 511 and 521 are provided with valves 513 and 523 for supplying or blocking water, respectively.

In addition, the water supply pipes 511 and 521 and the return pipes 512 and 522 connecting the first and second heat exchangers 520 and 530 may include a refrigerator 540 for cooling a heat source passing therebetween. ) Is installed, and as the heat source of the second heat exchanger 530 is cooled through the refrigerator 540, the temperature of the culture solution passing through the connection pipe 200 may be cooled. In more detail, when the temperature of the culture solution is increased, the freezer 540 reduces the culture solution to an appropriate temperature by measuring the temperature value.

In addition, a pump 524 is installed in the return pipe 522 to force circulation of water through the pump 524.

In addition, the steam boiler 510 is connected to the upper surface of the culture tank 100 through the steam supply pipe 514, the steam generated from the steam boiler 510 through the steam supply pipe 514 culture chamber It is supplied to the inside of the 110, the valve 514a for supplying or blocking steam is provided on the steam supply pipe 514.

In addition, a heating tube 550 is installed at the lower portion of the culture chamber 110 of the culture tank 100 to rapidly increase the temperature of the initial culture medium, and the heating tube 550 generates heat by electricity.

That is, the heating tube 550 is to cut off the power when the culture solution reaches the set temperature after raising the temperature of the culture solution rapidly.

The culture solution supply unit 600 is for supplying a culture solution into the culture chamber 110, and is installed through a culture solution supply pipe and a culture solution discharge pipe each including valves 610 and 620 on one side of the connection pipe 200. Inside, an impeller for stirring the culture solution is rotatably installed by a motor.

That is, when the culture medium supply unit 600 opens the valve 620 of the culture medium supply pipe, a part of the water passing through the connection pipe 200 is introduced into the culture medium supply unit 600 through the culture medium supply pipe, and the introduced water and the culture medium Is stirred by an impeller, and when the agitation is completed, the valve 620 of the culture medium supply pipe is blocked and the valve 610 of the culture medium discharge pipe is opened to allow the stirred culture solution to be connected to the culture chamber 110 through the connection pipe 200. To be supplied.

On the other hand, the culture solution supply unit 600 may be agitated by the high pressure air.

More specifically, the donut-shaped nozzle is installed inside the culture medium supply unit 600, the nozzle is formed with a plurality of injection holes in the lower end, the nozzle is supplied with high-pressure air from the air supply unit 700 to be described later the minute Air is injected through the pores to agitate the water and the culture.

Here, the culture solution supply unit 600 is selectively used according to the type of microorganism in the stirring method by the motor and the impeller and the stirring method by the air.

The air supply unit 700 is installed at the bottom to supply air according to the type of culture solution stored in the culture chamber 110, the halogen lamp 800 is installed outside the viewing window 140 according to the type of culture solution It will emit light.

That is, in order to create an environment necessary for the growth of the culture solution, air is supplied to the culture chamber 110 through the air supply unit 700 or light is supplied through the halogen lamp 800.

Here, the air supply unit 700 is provided with an air filter 710 for filtering the air sucked.

And the upper one side of the connecting pipe 200 is connected to the discharge pipe 900 for discharging the culture fluid passing through the connection pipe 200 to the outside, the solution passing through the discharge pipe 900 on the discharge pipe (900) A plurality of reservoirs 950 are installed for storing.

That is, when the growth of the culture solution stored in the culture chamber 110 is completed, it is stored until it is used in the reservoir 950 through the connection pipe 200 and the discharge pipe 900.

In addition, when the microbial culture is completed, the high temperature steam is circulated through the steam supply pipe 514 to the connection pipe 200 and the culture tank 100 to sterilize and wash.

Referring to the culture method of the microorganism culture apparatus having such a configuration as follows.

As shown in Figure 3, the microbial culture method is S10 step of supplying water to the culture tank 100, S20 step of circulating the water stored in the culture tank 100 through the connection pipe 200, circulated water S30 step of heating to a set temperature through the heating unit 500, S40 step of supplying the culture solution through the culture medium supply unit 600 to the culture tank 100, the air supply unit 700 and the halogen unit 800 S50 step of culturing the microorganisms in the culture medium by creating the conditions necessary for the growth of the culture medium through, S60 step of lowering the culture rate of the microorganisms through the freezer 540 when the culture is completed, and storage of the culture medium is reduced (950) In step S70 to store.

Referring to the microorganism culture method in more detail as follows.

First, step S10 connects the incubator 190 to any one of the plurality of culture tanks 100 and supplies water into the culture tank 100 through the incubator 190.

That is, when the valve 310 of the water supply pipe 300 of the incubator 190 is opened, water is supplied to the culture chamber 110 of the culture tank 100 through the water supply pipe 300 and the connection pipe 200, and the gauge ( When stored to a predetermined height inside the culture chamber 110 through 120 to close the valve 310 to block the supply of water.

Here, since the water supplied through the water supply pipe 300 passes through the monomolecular machine 320, the water molecules are minimized, thereby creating an optimal environment for the growth of microorganisms.

When the water is stored in the culture chamber 110 by the set height in step S10, by opening the valve 210, 220 of the connecting pipe 200, the pump 400 is operated to connect the water stored in the culture chamber 110 It circulates through the pipe 200 (step S20).

When the water is circulated according to the step S20, by operating the heating unit 500 to heat the water passing through the connection pipe 200 to 25 ° ~ 35 °.

That is, when the steam boiler 510 of the heating unit 500 is operated, the first heat exchanger 520 is heated by a heat source generated while the steam boiler 510 is heated, and the first heat exchanger ( The second heat exchanger 530 is heated by the heat source generated while the 520 is heated, and the second heat exchanger 530 is heated to heat the water passing through the connection pipe 200.

In addition, in the early stage of heating, heat is generated by supplying electricity to the heating tube 550 to quickly heat the water stored in the culture chamber 110. (Step S30).

When the water is heated to the set temperature according to the step S30, the operation of the culture medium supply unit 600 and at the same time to stop the operation of the heating tube 550, and then open the valve 620 heating to pass through the connection pipe 200 A portion of the prepared water is supplied to the culture solution supply unit 600.

Then, the water and the culture medium supplied into the culture medium supply unit 600 is stirred for a predetermined time through an impeller, and when the stirring is completed, the valve 620 of the culture medium supply pipe is blocked and at the same time the valve 610 of the culture medium discharge pipe. Open to supply the stirred culture solution with the water circulated through the connection pipe 200 into the culture chamber 110 (step S40).

When the culture solution set in the culture chamber 110 is supplied according to the step S40, the culture medium is supplied through the valve 610, and air or light necessary for culturing microorganisms contained in the supplied culture solution is supplied to the air supply unit 700 or halogen. Feed through 800 to greatly increase the microbial culture rate (step S50).

When the microbial culture is completed according to the step S50, the steam boiler 510 is stopped and at the same time the refrigerator 540 is operated to cool the heat sources of the first and second heat exchangers 520 and 530, and Cooling the culture medium passing through the connecting pipe 200 through to adjust the culture rate of the microorganisms (step S60).

That is, long-term preservation is possible by cooling the culture medium through the freezer 540 to prevent further growth.

When the growth of the culture solution is suppressed according to the step S60, the valve 210 of the connection pipe 200 is closed and the valve 910 of the discharge pipe 900 is opened to discharge the culture fluid circulating through the connection pipe 200. Either to discharge to the outside or store in the storage 950 (step S70).

Through such a process, microorganisms can be effectively cultured, and an incubator 190 is installed outside to automatically circulate and culture the culture solution, thereby improving productivity.

After the culturing of the microorganisms after the step S70 is completed, all of the culture solution stored in the culture chamber 110 is discharged. Thereafter, the high temperature steam of the steam boiler 510 is circulated to sterilize and wash the inside of the culture chamber 110.

That is, after operating the steam boiler 510 again, by opening the valve 514a of the steam supply pipe 514 to the steam generated in the steam boiler 510 inside the culture chamber 110 through the steam supply pipe 514. Sprayed to the washing, the washed water is discharged through a drain valve (not shown) installed in the connecting pipe (200).

In addition, when the washing operation is performed by the steam boiler 510, all the above-described valves are opened to sterilize and wash all of the tubes in which the culture medium and the water are circulated.

As mentioned above, although preferred embodiment of this invention was described in detail, the technical scope of this invention is not limited to the above-mentioned embodiment, It should be interpreted by the claim. At this time, those of ordinary skill in the art should consider that many modifications and variations are possible without departing from the scope of the present invention.

1 is a view showing a conventional microbial culture apparatus.

Figure 2 is a diagram schematically showing a microbial culture apparatus according to the present invention.

Figure 3 is a flow chart showing a microbial culture method according to the present invention.

<Explanation of symbols for the main parts of the drawings>

100: culture tank 190: incubator

200: connector 300: water supply pipe

400: pump 500: heating

510: steam boiler 520: the first heat exchanger

530: second heat exchanger 540: freezer

550: heating tube 600: culture medium supply unit

700: air supply unit 800: halogen lamp

900 discharge pipe 950 storage

Claims (14)

Culture tank to form a culture chamber therein, Supplying the culture tank with water and the culture medium and includes a culture incubator to circulate the supplied water and culture medium, The incubator, A connecting pipe having both ends connected to the upper and lower surfaces of the culture tank, a water supply pipe for supplying water to the culture chamber through the connection pipe, a pump for circulating water stored in the culture chamber through the connection pipe, It is composed of a heating unit for heating the water passing through the connecting tube, and a culture medium supply unit for supplying the culture solution into the culture chamber through the connecting tube, The heating unit, A steam boiler, a first heat exchanger connected to the steam boiler so that heat exchange is possible, and one side of the second heat exchanger connected to the heat exchanger so as to be capable of heat exchange, and the other side of the steam heat exchanger is connected to the first heat exchanger. Equipped, The steam boiler is microbial culture apparatus characterized in that it is connected through the steam supply pipe and the upper surface of the culture tank to supply steam into the culture chamber. delete The method of claim 1, Microbial culture apparatus, characterized in that between the first and second heat exchanger is provided with a refrigerator for cooling the heat source circulating therebetween. The method of claim 1, Microbial culture apparatus, characterized in that the heating chamber for heating the culture solution in the culture chamber of the culture tank. The method of claim 1, The microorganism culture apparatus, characterized in that the lower surface of the culture tank is connected to the air supply for supplying air to the culture chamber. The method of claim 1, Microorganism culture apparatus characterized in that a halogen lamp for generating light in the viewing window formed on the outside of the culture tank. The method of claim 1, Microbial culture apparatus, characterized in that the culture tank is further provided with a heating tube. The method of claim 1, The culture tank further comprises a spray nozzle for injecting the culture solution passed through the connecting pipe to the culture chamber. The method of claim 1, The culture tank is a microbial culture apparatus, characterized in that provided with a gas removal unit for discharging the gas generated in the culture chamber. The method of claim 1, The discharge pipe is connected to the discharge pipe for discharging the culture fluid passing through the connection pipe to the outside, The microbial culture apparatus, characterized in that a plurality of reservoirs for storing the solution passing through the discharge pipe is installed on the discharge pipe. A culture tank in which a culture chamber is formed, and an incubator for supplying water and culture liquid to the culture tank, wherein the incubator is connected to the culture tank, and a water supply pipe for supplying water to the culture chamber through the connection pipe; It comprises a pump for circulating the water stored in the culture chamber through the connecting tube, a heating unit for heating the water passing through the connecting tube, and a culture medium supply unit for supplying the culture solution into the culture chamber through the connecting tube, The heating unit includes a steam boiler, a first heat exchanger connected to the steam boiler so as to exchange heat, and one side connected to the connection pipe so as to exchange heat, and the other side connected to the first heat exchanger to enable heat exchange. 2 is provided with a heat exchanger, the culture tank is a method for culturing microorganisms by a microorganism cultivation apparatus is provided with an air supply unit and halogen, etc. Come on. S10 step of supplying and storing water to the culture chamber of the culture tank through the water supply pipe and the connection pipe, S20 step of circulating the water stored in the culture chamber through the connection pipe by the pumping force of the pump installed in the connection pipe after the step S10, Operating a steam boiler during the step S20 to heat the water passing through the connection pipe based on a heat source transferred through the first heat exchanger and the second heat exchanger; S40 step of supplying the culture solution stored in the culture solution supply unit installed in the connecting tube to the culture chamber through the connecting tube when the water in the culture chamber rises to the set temperature as the step S30, Microorganism culture method comprising the step of culturing the microorganisms in the culture medium by supplying air or light to the culture chamber through the air supply or halogen, etc. according to the type of culture medium during the step S40. The method of claim 11, The step S30 further comprises the step of generating heat by supplying electricity to the heating tube to improve the heating rate of the water stored in the culture chamber, When the culture of the microorganisms is completed after the above step, the steam boiler is stopped and at the same time, the refrigerator is operated to cool the heat sources of the first and second heat exchangers and connect the connection pipe based on the cooled heat sources. Microbial culture method comprising the step of adjusting the microbial culture rate by cooling the culture medium to pass further. The method of claim 12, When the culture rate of the microorganism is controlled after the step S60, the microorganism culture method further comprising the step S70 of supplying and storing the culture solution in a reservoir connected to the connecting tube. The method of claim 13, When the culture of the microorganisms is completed after the step S70, the microorganism culture method further comprises the step S80 of sterilizing and washing by circulating the high temperature steam of the steam boiler.

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