KR102283140B1 - Microbial incubator - Google Patents

Abstract

The present invention makes it possible to cultivate and use microorganisms necessary for crops, agriculture, and others, and to cultivate a small amount of various microorganisms, and to minimize installation and maintenance costs without requiring much installation space. It relates to a microorganism culture apparatus;
a device body having a polygonal shape; an upper cap and a lower cap closing the upper and lower portions of the device body; an inoculation manhole installed in the front part of the upper cap to inoculate the medium of the microorganism to be cultured; a cleaning manhole installed on the front of the device body to clean the inside; a drain valve installed on the lower cap to discharge cultured microorganisms; a supply means for supplying raw water and oxygen necessary for culturing microorganisms by being installed on the device body; a heating means for heating and sterilizing the supplied raw water; a cooling means for cooling the sterilized raw water to a temperature suitable for medium inoculation; agitating means for culturing microorganisms by evenly mixing raw water and medium; a light transmitting means that enables photosynthesis in the microbial culture process; It is characterized in that it comprises a controller for controlling the supply means, heating means, cooling means, stirring means and light projection means mounted on one side of the device body.

Description

Microbial culture device {MICROBIAL INCUBATOR}

The present invention relates to an apparatus for culturing microorganisms, and more particularly, to a microorganism culture apparatus capable of facilitating the cultivation of microorganisms, particularly chlorella, having the efficacy of soil improvement, growth promotion, disease prevention, quality improvement, and alleviation of residual pesticides about the provision of

Microorganisms enhance the effectiveness of manure, improve the separation ability of residual pesticides, improve soil productivity through restoration of soil productivity, increase the germination rate and promote growth of crops, enhance photosynthesis in the growth process, prevent various diseases and pests, and In addition to improving the yield and marketability by enhancing the sugar content, hardness and size of fruits and vegetables, the excellent performance of adsorbing heavy metals in pesticides performed in the process of growing crops has been proven, and it is widely used in agricultural and livestock and horticultural farms. .

These microorganisms are cultivated and used using a dedicated microbial culturing apparatus, and are developed and used in various forms in the prior art. Among these microbial culturing apparatuses, the prior art through the patent literature is as follows.

Patent Document 1 discloses a microorganism culturing apparatus for culturing microorganisms;

a culture chamber 10 provided with an inlet 11 to which a culture material is supplied and an outlet 12 through which the culture material in which the microorganisms are cultured is discharged, while an exhaust port 13 through which water vapor is discharged to the outside; a stirrer 20 that is rotatably installed in the inner space of the culture chamber 10 and supplies hot air to the inner space of the culture chamber 10 while mixing and agitating the culture material; a driving unit 30 providing a driving force of the motor 31 to the stirring unit 20; and a gate 40 for selectively opening and closing the outlet 12 through which the culture material in which the microorganisms are cultured in the culture chamber 10 is discharged; and the culture chamber 10 has a steam chamber 15 on its outer periphery It has a dual structure formed, and the heat insulating material 16 is wrapped around the outer periphery of the steam chamber 15 and installed, and the steam pipes 15a are connected and installed on both upper sides of the steam chamber 15, respectively.

Patent Document 2 discloses a culture tank partitioned into a culture solution filling part filled with a culture solution to a certain depth therein and an air layer part formed on the upper part of the culture solution filling part, and hot air into the culture tank to keep the temperature inside the culture tank constant A heater for supplying, a pump for sucking the culture solution of the culture solution filling part, a circulation pipe connected to the pump to circulate the culture solution inside the culture tank, and the negative pressure generated while the culture solution passes through the circulation pipe. is provided with an air suction pipe having an inlet hole that communicates with the inside of the circulation pipe in the air layer part so that the air is sucked in, and penetrates back and forth so that aeration of the culture medium and the air sucked from the air suction pipe is actively made, so that at least one layer or more It is configured to include an aeration means provided with an aeration active pipe which is overlapped to be spaced apart by a predetermined width and disposed to face the inlet hole of the air suction pipe.

Patent Document 3 discloses a stirred tank for mixing and stirring a culture agent such as molasses and a microorganism, a culture tank for culturing microorganisms by accommodating a culture solution that is a mixture of a culture agent and a microorganism stirred in the stirred tank, and the stirred tank; A first pump motor installed between the culture tanks to transfer the stirred culture solution from the stirred tank to the culture tank and circulate the culture solution up and down in the culture tank, and a second pump motor for discharging the microorganisms cultured in the culture tank to the outside and a steam pipe installed in the stirring tank to dissolve the culture medium injected into the stirring tank.

Patent No. 10 - 0972755 - 0000 Patent No. 10 - 1025339 - 0000 Patent No. 10 - 1061424 - 0000

None.

Among the prior art as described above, Patent Document 1 installs a hollow shaft in the axial center direction of a cylindrical culture chamber lying in the longitudinal direction, mounts a stirring blade, and supplies hot air through the hollow shaft and simultaneously provides a steam chamber in the culture chamber. Formed so as to maintain the temperature required for culture, Patent Document 2 pumps in and circulates the culture medium in which the microorganisms are cultured inside the culture tank, and smoothly mixes air into the circulating culture liquid to increase the amount of dissolved oxygen By increasing it, it promotes the active activity of microorganisms, and at the same time, it is configured so that the culture solution is sprayed to the upper surface of the culture solution to maintain the dissolved oxygen necessary for culturing microorganisms, and it can be confirmed that Patent Document 3 is a dual configuration of a stirred tank and a culture tank. there is.

Patent Document 1 and Patent Document 3 as described above require a lot of space for installation because they are bulky to install, so it is possible to use them in large farms or agricultural corporations, but it is not necessary to install and use them in small farms. Because it takes a lot of space and cost, it becomes impossible to use it realistically.

In the case of Patent Document 2, it can occupy a small installation space, but since it is a configuration that continuously circulates the culture medium by a pump for culturing microorganisms, it is questionable to actually increase the temperature and dissolved oxygen required for culturing microorganisms. and, since it is difficult in reality to meet the conditions suitable for culturing microorganisms, the culturing efficiency of microorganisms is questioned.

In the case of the above patent documents, the device is oversized, occupies a lot of installation space, and the initial installation cost is excessive, and there is considerable difficulty in maintenance such as continuously operating it, and it is difficult to apply it in a small farmhouse. Of course, in the case of a small microbial culturing apparatus, various problems occur, such as the fact that the culturing efficiency is lowered, making it unsuitable for culturing practical microorganisms and using them in farms.

Accordingly, in the present invention, as an invention to solve the above problems, a square corner portion having a front surface 104, a rear surface 105, and both sides 106 is a corner surface 107 having a length shorter than the length of each surface. a device body 101 that is connected to a polygonal body and has a high height compared to the width;

an upper cap 102 and a lower cap 103 protruding upwardly and downwardly protruding from the upper and lower portions of the device body 101;

an inoculation manhole 108 installed in the front part of the upper cap 102 to inoculate the medium of the microorganism to be cultured;

a cleaning manhole 109 installed in the central portion of the front surface 104 to clean the inside;

a drain valve 110 installed on the lower cap 103 to discharge cultured microorganisms;

a supply means 120 installed on the device body 101 to supply raw water and oxygen necessary for culturing microorganisms;

a heating means 140 for heating and sterilizing the raw water supplied by the supply means 120;

a cooling means 160 for cooling the sterilized raw water to a temperature suitable for medium inoculation;

Stirring means 180 for culturing microorganisms by evenly mixing the raw water and the medium;

Light-transmitting means 200 that enables photosynthesis in the process of culturing microorganisms;

It includes a controller 115 mounted on one side of the device body 101 to control the supply means 120 , the heating means 140 , the cooling means 160 , the stirring means 180 and the light projection means 200 . Microbial culture apparatus, characterized in that.

By repeating the above operations, it is possible to cultivate and use microorganisms necessary for crops, farming, and others, and it is possible to culture a variety of microorganisms with a small amount, and installation and maintenance costs are reduced without requiring a lot of installation space. Minimized goals can be achieved.

Since the present invention is small and provides ease of movement, it can be installed and used regardless of place, so it can be used by culturing suitable microorganisms when necessary at low cost in horticulture, flowers, livestock, etc., as well as small farms, for convenience of users. It has the effect of reducing the economic burden by increasing and reducing the various costs required for maintenance and management.

The present invention is a small capacity and enables the culturing of various microorganisms such as chlorella, photosynthetic bacteria, bacillus, lactic acid bacteria and useful microorganisms, and optimizes conditions suitable for microorganisms to be cultured through heating, heating, sterilization, stirring, aeration and light transmission. It has various effects, such as increasing the quality of the culture equipment and enhancing external competitiveness, as it can be efficiently cultured within a short period of time.

1 is an external perspective view showing the front of a microorganism culture apparatus to which the technology of the present invention is applied.
Figure 2 is an external perspective view showing the rear surface of the microorganism culture apparatus to which the technology of the present invention is applied.
Figure 3 is an external configuration diagram showing a plan view of the microorganism culture apparatus to which the technology of the present invention is applied.
Figure 4 is a block diagram of a cross-sectional state showing a microorganism culture apparatus to which the technology of the present invention is applied.
Figure 5 is a cross-sectional view showing a portion A of the microorganism culture apparatus to which the technology of the present invention is applied.
Figure 6 is an exemplary view showing the state of use of the microorganism culture apparatus to which the technology of the present invention is applied.

Hereinafter, the preferred configuration and operation of the present invention for achieving the above object will be described with reference to the accompanying drawings.

Figure 1 is an external perspective view showing the front of the microorganism culturing apparatus to which the technology of the present invention is applied, Figure 2 is an external perspective view showing the back of the microorganism culturing apparatus to which the technology of the present invention is applied, Figure 3 is a microorganism to which the technology of the present invention is applied An external configuration diagram showing a plane of the culture apparatus, FIG. 4 is a configuration diagram of a cross-sectional state showing a microorganism culture apparatus to which the technology of the present invention is applied, and FIG. 5 is an extract of part A of the microorganism culture apparatus to which the technology of the present invention is applied. The cross-sectional view shown, Figure 6 will be described together as an exemplary view showing the state of use of the microorganism culture apparatus to which the technology of the present invention is applied.

The microorganism culturing apparatus 100 to which the technology of the present invention is applied is provided with a device body 101 that is high in height compared to the width in a polygonal shape such as a pentagon, hexagon, or octagon to provide ease of agitation, and the device The upper and lower portions of the body 101 are closed with an upper cap 102 and a lower cap 103 that protrude upward and protrude downward.

The device body 101, the front 104, the back side 105, each side in the stirring process by connecting the corner portion of the square shape having a side 106 with a corner surface 107 having a shorter length than the length of each side. To provide a shape that can increase the stirring efficiency by hitting the connection part.

An inoculation manhole 108 for inoculating a medium of a microorganism to be cultured is installed in the front part of the upper cap 102 of the device body 101 so as to be able to open and close, and in the central part of the front 104, a cleaning manhole for cleaning the inside (109) is installed so as to be able to open and close, and a drain valve (110) for discharging the cultured microorganisms is installed in the center of the lower cap (103).

The device body 101 has a supply means 120 for supplying raw water and oxygen necessary for culturing microorganisms, a heating means 140 for heating and sterilizing the raw water supplied by the supply means 120, and the sterilized A cooling means 160 for cooling the raw water to a temperature suitable for inoculation of the medium, a stirring means 180 for culturing microorganisms by evenly mixing the raw water and the medium, and a light transmitting means 200 for photosynthesis in the process of culturing microorganisms ), including

A controller 115 for controlling the supply means 120 , the heating means 140 , the cooling means 160 , the stirring means 180 and the light projection means 200 is mounted on one side of the device body 101 . Of course, a support leg 118 having a stopper 116 for limiting movement and a caster 117 for providing ease of movement on the lower side of the device body 101 is mounted at the lower end.

The supply means 120 installs a water supply pump 122 for supplying raw water to the supply plate 121 protruding to the rear of the upper cap 102, and the water supply pump 122 and the device body 101 inside. Until the water supply pipe 123 is connected so that raw water can be filled, the operation of the water supply pump 122 can be stopped by sensing the water level sensor provided inside the water supply pipe 123 or the device body 101.

An air pump 124 is installed on the side of the water pump 122 to connect the air tube 125 to the inside of the device body 101 to supply oxygen necessary for culturing microorganisms, and The discharge pipe 126 is further connected to discharge air bubbles or gas to the outside.

In addition to the water supply pipe 123, the air pipe 125, and the exhaust pipe 125, a cooling water line 127 for supplying cooling water to the device body 101 may be further installed, and the water supply pipe 123 and the air pipe 125. , the exhaust pipe 125 and the coolant line 127 are equipped with a filter body 128 to filter foreign substances, etc., and have an indicator that can visually check the amount of raw water, cooling water and air supplied incidentally. you will be able to make

The heating means 140, by heating the supplied raw water to a temperature of 80 ~ 90 ℃ to a 10Kw or more class sterilization heater 141 that kills general bacteria installed on one side near the bottom of the device body 101, the sterilization At the opposite position of the heater 141, a 1Kw-class heating heater 142 is installed so that the raw water inoculated with the medium can be continuously maintained at a temperature preferred by microorganisms.

The cooling means 160 is for cooling the raw water heated by the sterilization heater 141 to a temperature suitable for medium inoculation (about 35° C.), and a cooling jacket 161 that is airtight to the lower cap 103 . is installed, and a cooling coil 162 is installed inside the cooling jacket 161 to be controlled by the controller 115 .

The stirring means 180 is provided with a stirring shaft 181 that descends from the center of the upper cap 102 to a position below the device body 101, and the stirring shaft 181 is mounted on the upper cap 102. To be maintained in the shaft housing (182).

The stirring shaft 181 is equipped with a stirring motor 185 having a gear box 183 integrally to rotate the stirring shaft 181, and the stirring shaft 181 located inside the device body 101. A plurality of agitating impellers 186 are mounted thereto and configured to stir evenly.

The light projecting means 200 protrudes circular or rectangular flanges 201 on both sides of the upper cap 102 and on the back 104 and both sides 106 of the device body 101, and on the flange 201 In order to prevent the leakage of microorganisms, the light-transmitting plate 205 made of a transparent material having the airtight material 202 interposed on both sides is closely adhered and fixed with the intermittent ring 206 .

An LED-type floodlight 207 is installed on the floodlight plate 205, and the light lit according to the type of microorganism to be cultured by the controller 115 is varied, and the illuminance can be varied to increase the cultivability. configure it so that

In the present invention, the floodlight 207 is exemplified to arrange one in the center of the floodlight plate 205 , but several smaller-sized floodlight rides 207 may be disposed, and have the same size as the floodlight plate 205 . It can be implemented in various ways, such as disposing the floodlight 207 of the.

The process of culturing microorganisms using the microbial culture apparatus to which the technology of the present invention is applied as described above is as follows.

First, when the water supply pump 122 is operated to supply raw water to the inside of the device body 101 through the water supply pipe 123, the water supply is stopped after an appropriate amount is filled by the water level sensor, and after the water is supplied, the sterilization heater 141 is turned on. It works to kill common bacteria by heating the raw water to a temperature of 80 ~ 90℃, which is a temperature that does not boil.

In this state, after replenishing the cooling water through the cooling water line 127, the cooling coil 162 inside the cooling jacket 161 installed on the lower cap 103 is operated to culture the microorganisms in the raw water at an elevated temperature together with the cooling water and Cooling is performed to a temperature suitable for inoculation (temperature around 35° C.).

When the cooling of the raw water is completed, the inoculation manhole 108 provided in the upper cap 102 is opened to inoculate the medium, and then the boiling manhole 108 is closed, and the stirring motor 185 constituting the stirring means 180 is operated. Aeration, heating, and light transmission are carried out so that the medium and raw water are evenly mixed, and conditions suitable for culturing microorganisms are maintained.

The stirring shaft 181 installed in the center of the inner shaft of the apparatus body 101 is rotated by the stirring motor 185, and the stirring impeller 186 provided in the stirring shaft 181 serves to stir, and at the same time The culture medium stirred by the stirring impeller 186 collides with the edge surface 107 constituting the equipment body 101, resulting in more even stirring and mixing.

In the case of aeration, the air is supplied by the air pump 124 through the air pipe 125 that is lowered from the upper cap 102 to the inside of the device body 101 as for the supply of dissolved oxygen necessary for culturing the living organisms. , it will be natural to pass through the filter body 128 to prevent the inflow of foreign substances.

Of course, even when raw water is supplied, it will be natural for foreign substances to be introduced in a filtered state by the filter body 128 provided in the cooling water line 127 between the water supply pump 122 and the water supply pipe 123, and to cultivate microorganisms. Gas that may be generated in the process is discharged through the discharge pipe 126 to ensure safety.

To maintain the heating, the heating heater 142 installed inside the device body 101 opposite the sterilization heater 141 is operated to gradually raise the temperature to a suitable temperature for culturing microorganisms without increasing the temperature abruptly, and to continuously maintain it. will do

In addition, in the case of microorganisms requiring photosynthesis by operating the floodlight 207 provided on the floodlight plate 105 installed on the rear surface 105 and both side surfaces 106 of the upper cap 102 and the device body 101, Turn it on so that the culture can be carried out smoothly.

The culture of the microorganism usually takes about 5 days (4 nights and 5 days) based on 500 liters. During this period, continuous stirring, aeration, and light transmission under heating can be used to culture the microorganisms in an optimal state. , this may be manually operated by the user, but it will be of course that it can be automatically performed from start to finish by the controller 115 according to the type of microorganism to be cultured.

When the culture of microorganisms is completed as described above, microorganisms are extracted and used through the drain valve 110 , and the inside of the apparatus body 101 through the cleaning manhole 109 installed on the front surface 104 of the Jangji body 101 . It is enough to clean the microbial residues attached to it so that it does not interfere with culturing the next microorganism.

By repeating the above operations, it is possible to cultivate and use microorganisms necessary for crops, farming, and others, and it is possible to culture a variety of microorganisms with a small amount, and installation and maintenance costs are reduced without requiring a lot of installation space. It has the advantage that it can be minimized.

100; Microbial culture apparatus 101; device body
102; upper cap 103; lower cap
108; inoculation manhole 109; cleaning manhole
110; drain valve 115; controller
120; supply means 123; feed pipe
125; air tube 126; discharge pipe
127; coolant line 128; filter body
140; heating means 141; sterilization heater
142; heating heater 160; cooling means
161; cooling jacket 162; cooling coil
180; stirring means 181; stirring shaft
185; stirring motor 186; stirring impeller
200; light transmitting means 201; flange
205; floodlight 207; floodlight

Claims (6)

The front 104, the back 105, and the rectangular corner having both sides 106 are connected to the corner surface 107 having a length shorter than the length of each side, so that the device body 101 is a polygonal body with a high height compared to the width )Wow; an upper cap 102 and a lower cap 103 that protrude upwardly and project downwardly on the upper and lower portions of the device body 101; an inoculation manhole 108 installed in the front part of the upper cap 102 to inoculate the medium of the microorganism to be cultured; a cleaning manhole 109 installed in the central portion of the front surface 104 to clean the inside; a drain valve 110 installed on the lower cap 103 to discharge cultured microorganisms; a supply means 120 installed on the device body 101 to supply raw water and oxygen necessary for culturing microorganisms; a heating means 140 for heating and sterilizing the raw water supplied by the supply means 120; a cooling means 160 for cooling the sterilized raw water to a temperature suitable for medium inoculation; Stirring means 180 for culturing microorganisms by evenly mixing the raw water and the medium; Light-transmitting means 200 that enables photosynthesis in the process of culturing microorganisms; It includes a controller 115 mounted on one side of the device body 101 to control the supply means 120 , the heating means 140 , the cooling means 160 , the stirring means 180 and the light projection means 200 . do;
The supply means 120 includes a water supply pump 122 installed to supply raw water to the supply plate 121 protruding to the rear of the upper cap 102; a water supply pipe 123 connecting to the inside of the water pump 122 and the device body 101; an air pipe 125 connected to an air pump 124 installed on the side of the water pump 122 and positioned inside the device body 101 to supply oxygen necessary for culturing microorganisms; a discharge pipe 126 for connecting air bubbles or gases generated in the process of culturing microorganisms to the outside to be discharged; a cooling water line 127 installed to supply cooling water to the device body 101; The water supply pipe 123, the air pipe 125, the exhaust pipe 125, and the cooling water line 127 further includes a filter body 128 for filtering foreign substances;
The light projecting means 200, the upper cap 102, both sides of the device body 101, the rear surface 104 and the flange 201 protruding from both sides (106); a transparent light-transmitting plate 205 that is closely attached to the flange 201 with an airtight material 202 interposed therebetween on both sides to prevent the leakage of microorganisms; an intermittent ring 206 intermittently intermitting the flange 201 and the floodlight plate 205; The apparatus for culturing microorganisms, characterized in that it further comprises a floodlight (207) installed on the floodlight plate (205) and turned on according to the type of microorganism to be cultured by the controller (115). delete delete delete delete delete

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