KR20200122578A - Microbial incubator - Google Patents

Abstract

The present invention relates to a microbial culture device which makes it possible to culture and use microorganisms necessary for crops, farming, and other purposes, to culture various microorganisms in a small amount, and to minimize installation and maintenance costs without requiring a large installation space, wherein the microbial culture device comprises: a polygonal device body; an upper cap and a lower cap closing upper and lower portions of the device body; an inoculation manhole installed to inoculate a medium of microorganisms to be cultured in front of the upper cap; a cleaning manhole installed to clean the inside of the device body in front thereof; a drain valve installed on the lower cap to discharge the cultured microorganisms; a supply means for supplying raw water and oxygen required for culture of microorganisms by being installed on the device body; a heating means for sterilizing by heating the supplied raw water; a cooling means for cooling the sterilized raw water to a temperature suitable for medium inoculation; a stirring means for culturing microorganisms by evenly mixing the raw water and medium; a light-transmitting means that enables photosynthesis in the process of microbial culture; and a controller mounted on one side of the device body to control the supply means, heating means, cooling means, stirring means, and light-transmitting means.

Description

Microbial culture device {MICROBIAL INCUBATOR}

The present invention relates to a microbial culture apparatus, and more particularly, a microbial culture apparatus capable of facilitating the cultivation of microorganisms, particularly chlorella, having an effect of improving soil, promoting growth, preventing diseases and improving quality, and reducing residual pesticides. It is about the provision of.

Microorganisms enhance the effectiveness of manure and improve the classification ability of residual pesticides, improve the intellectual power by restoring the productivity of the soil, increase the germination rate of crops and promote growth, enhance photosynthesis in the growth process, prevent various diseases and pests, and It has proven its excellent performance in adsorbing heavy metals in pesticides carried out in the process of growing crops, as well as improving the harvestability and marketability by improving the sugar content, hardness and size of fruits and vegetables, which are widely used in recent years in agricultural and livestock farming and horticultural farms. .

These microorganisms are cultivated and used using a dedicated microorganism cultivation apparatus, and the situation has been developed and used in various forms even in the prior art. The prior art of these microorganism cultivation apparatuses through patent literature is as follows.

Patent Document 1 is a microbial culture apparatus for proliferating and culturing microbes;

A culture chamber 10 provided with an inlet 11 through which the culture material is supplied and an outlet 12 through which the culture material in which microorganisms are cultured is discharged, and an exhaust port 13 through which water vapor is discharged to the outside; A stirring unit 20 which is rotatably installed in the inner space of the culture chamber 10, stirs and stirs the culture material, and supplies hot air to the inner space of the culture chamber 10; A driving unit 30 providing a driving force of the motor 31 to the stirring unit 20; Including a gate 40 for selectively opening and closing the outlet 12 through which the culture material in which the microorganisms are cultured is discharged in the culture chamber 10, the culture chamber 10 includes a steam chamber 15 at the outer periphery. It is composed of a formed double structure, the insulating material 16 is wrapped around the outer periphery of the steam chamber 15 and installed, and the steam pipes 15a are connected and installed on both upper sides of the steam chamber 15, respectively.

Patent Document 2, a culture tank partitioned by a culture solution filling portion filled with a culture solution to a certain depth inside and an air layer portion formed above the culture solution filling portion, and a hot air inside the culture tank to maintain a constant temperature inside the culture tank. A heating device for supplying, a pump for sucking the culture solution of the culture solution filling part, a circulation pipe connected to the pump to circulate the culture solution inside the culture tank, and air by negative pressure generated while the culture solution passes through the circulation tube. An air suction pipe having an inlet hole communicating from the air layer portion to the inside of the circulation pipe is provided so that the air is sucked, and at least one layer is penetrated forward and backward so that aeration between the culture liquid and the air sucked from the air suction pipe is actively performed. The configuration includes an aeration means provided with an aeration activating tube arranged to face the inlet hole of the air intake tube by overlapping a predetermined width.

Patent Document 3 includes a stirring tank for mixing and stirring a culture agent such as molasses and a microorganism, a culture tank for culturing microorganisms by accommodating a culture solution that is a mixture of the culture agent and microorganisms stirred in the stirring tank, and the stirring tank A first pump motor installed between the culture tanks to transfer the culture solution stirred in the stirring tank to the culture tank and circulate the culture solution in the culture tank up and down, and a second pump motor to discharge microorganisms cultured in the culture tank to the outside. And, it is installed in the stirring tank and consists of a steam tube for dissolving the culture medium introduced into the stirring tank.

Patent No. 10-0972755-0000 Patent No. 10-1025339-0000 Patent No. 10-1061424-0000

None.

Among the prior art as described above, Patent Document 1 is to install a hollow shaft in the axial center direction of a cylindrical culture chamber lying in the longitudinal direction to mount a stirring blade, supply hot air through the hollow shaft, and simultaneously provide a steam chamber to the culture chamber. In order to maintain the temperature required for cultivation, Patent Document 2 sucks and circulates the culture medium in which microorganisms are cultivated inside the culture tank by means of a pump, and the amount of dissolved oxygen is determined by allowing air to be smoothly mixed into the circulating culture solution. By increasing the active activity of microorganisms, the culture medium is configured to be sprayed above the water surface of the culture medium to maintain dissolved oxygen required for cultivation of microorganisms, and Patent Document 3 confirms that the agitating tank and the culture tank are dually configured. have.

Patent Literature 1 and Patent Literature 3 as described above require a lot of installation space due to the large installation, so they can be used in places with a scale such as large farms or farming corporations, but they are not necessary to install and use in small farms. Since it takes a lot of space and cost, practical use becomes impossible.

In the case of Patent Document 2, the installation space can be occupied small, but since it is a configuration in which the culture medium is continuously circulated by a pump to cultivate microorganisms, it is questionable to increase the temperature and dissolved oxygen required for cultivation of microorganisms. And, since it is practically difficult to meet the conditions suitable for cultivation of microorganisms, the cultivation efficiency of microorganisms is suspected.

In the case of the patent documents as described above, the device is enlarged and takes up a lot of installation space and requires excessive initial installation cost, as well as considerable difficulty in maintenance such as continuous operation, and is applied to small farms. In addition, in the case of a small-sized microbial culture apparatus, there are various problems, such as a decrease in culture efficiency, and thus not suitable for use in farms by culturing practical microbes.

Accordingly, in the present invention, as invented to solve the above problems, a rectangular corner portion having a front surface 104, a rear surface 105, and both side surfaces 106 has a length shorter than the length of each surface. A device body 101 having a height compared to the width of a polygonal body by connecting it;

An upper cap 102 and a lower cap 103 protruding upwardly and downwardly protruding from the upper and lower portions of the device body 101;

An inoculation manhole 108 installed in the front portion of the upper cap 102 to inoculate a medium of microorganisms to be cultured;

A cleaning manhole 109 installed in the central portion of the front 104 to clean the interior;

A drain valve 110 installed on the lower cap 103 to discharge the cultured microorganisms;

A supply means (120) installed on the device body (101) for supplying raw water and oxygen required for cultivation of microorganisms;

A heating means 140 for heating and sterilizing the raw water supplied by the supply means 120;

Cooling means 160 for cooling the sterilized raw water to a temperature suitable for inoculation of the medium;

A stirring means 180 for evenly mixing raw water and medium to cultivate microorganisms;

Transmitting means 200 to enable photosynthesis during microbial culture;

Includes a controller 115 mounted on one side of the device body 101 to control the supply means 120, the heating means 140, the cooling means 160, the stirring means 180, and the light transmitting means 200. Microbial culture device, characterized in that.

By repeating the above operation, it is possible to cultivate and use microorganisms necessary for crops, farming, and other purposes, and it is possible to cultivate various microorganisms in a small amount, and installation and maintenance costs are reduced without requiring a lot of installation space. It is possible to achieve a goal that can be minimized.

Since the present invention is small and easy to move, it can be installed and used regardless of the location, so it is possible to cultivate and utilize suitable microorganisms when necessary at low cost not only in small-scale farmers, but also in horticulture, flowers, and livestock. It has the effect of reducing the economic burden by increasing it and reducing the overall cost required for maintenance and management.

The present invention enables the cultivation of various microorganisms such as chlorella, photosynthetic bacteria, Bacillus bacteria, lactic acid bacteria and useful microorganisms in small doses, while heating, heating, sterilization, agitation, aeration and light transmission to optimize conditions suitable for the microorganisms to be cultured. By making it possible to squeeze it, it has a variety of effects, such as being able to efficiently cultivate squeezed in time, thereby enhancing the quality of the culture device and enhancing external competitiveness.

1 is an exterior perspective view showing the front of a microorganism culture apparatus to which the technology of the present invention is applied.
Figure 2 is an exterior perspective view showing the rear surface of the microorganism culture apparatus to which the technology of the present invention is applied.
Figure 3 is an exterior configuration diagram showing a plan view of a microorganism culture apparatus to which the technology of the present invention is applied.
Figure 4 is a configuration diagram of a cross-sectional state showing a microorganism culture apparatus to which the technology of the present invention is applied.
Figure 5 is a cross-sectional view showing an excerpt A portion of the microorganism culture apparatus to which the technology of the present invention is applied.
Figure 6 is an exemplary view showing a state of use of the microorganism culture device to which the technology of the present invention is applied.

Hereinafter, a preferred configuration and operation of the present invention for achieving the above object will be described with reference to the accompanying drawings.

1 is an exterior perspective view showing the front side of a microorganism culturing apparatus to which the technology of the present invention is applied, FIG. 2 is an exterior perspective view showing the rear side of a microorganism culturing apparatus to which the technology of the present invention is applied, and FIG. 3 is a microorganism to which the technology of the present invention is applied An external configuration diagram showing a plan view of a culture device, FIG. 4 is a configuration diagram in a cross-sectional state showing a microorganism culture device to which the technology of the present invention is applied, and FIG. 5 is an excerpt of part A of the microorganism culture device to which the technology of the present invention is applied. A cross-sectional view, Figure 6 will be described together as an exemplary view showing the state of use of the microorganism culture apparatus to which the technology of the present invention is applied.

The microorganism cultivation apparatus 100 to which the technology of the present invention is applied is provided with a device body 101 having a height compared to the width of a polygonal body such as a pentagonal, hexagonal, or octagonal so as to provide ease of stirring, and the device The upper and lower portions of the body 101 are closed with an upper cap 102 and a lower cap 103 protruding upward and downward.

The device body 101 connects square corners having a front surface 104, a rear surface 105, and both sides 106 to a corner surface 107 having a length shorter than the length of each surface, It is to provide a form that can hit the connection and increase the stirring efficiency.

An inoculation manhole 108 for inoculating a medium of microorganisms to be cultured is installed in a front part of the upper cap 102 of the device body 101 so as to be opened and closed, and a cleaning manhole for cleaning the inside of the front part 104 A drain valve 110 for discharging the cultured microorganisms is installed in the center of the lower cap 103 and opened and closed.

The device body 101 includes a supply unit 120 for supplying raw water and oxygen required for cultivation of microorganisms, a heating unit 140 for heating and sterilizing the raw water supplied by the supply unit 120, and sterilized Cooling means 160 for cooling the raw water to a temperature suitable for inoculation of the medium, stirring means 180 for cultivating microorganisms by evenly mixing the raw water and the medium, and a light transmitting means 200 for enabling photosynthesis in the process of culturing microorganisms. ).

One side of the device body 101 is equipped with a controller 115 for controlling the supply means 120, the heating means 140, the cooling means 160, the stirring means 180 and the light transmitting means 200. As a matter of course, a support leg 118 having a stopper 116 for limiting movement and a caster 117 for providing ease of movement is mounted on the lower side of the device body 101.

The supply means 120 is provided with a feed pump 122 for supplying raw water to a feed plate 121 protruding to the rear of the upper cap 102, and the feed pump 122 and the device body 101 Until the water supply pipe 123 is connected so that raw water can be filled, and the operation of the water supply pump 122 can be stopped by detection of a water level sensor provided in the water supply pipe 123 or the device body 101.

An air pump 124 is installed on the side of the feed water pump 122 to connect the air pipe 125 to the inside of the device body 101 to supply oxygen required for culturing microorganisms. The discharge pipe 126 is further connected to discharge air bubbles or gases to the outside.

In addition to the water supply pipe 123, the air pipe 125, and the exhaust pipe 125, a cooling water line 127 for supplying cooling water to the device body 101 may be further installed, and the water supply pipe 123 and the air pipe 125 , The exhaust pipe 125 and the cooling water line 127 are further equipped with a filter body 128 to filter foreign substances, and have an indicator that can visually check the amount of raw water, cooling water and air supplied incidentally. I will be able to do it.

The heating means 140 heats the supplied raw water to a temperature of 80 to 90°C to kill common bacteria by installing a sterilization heater 141 of 10 Kw or higher at one side near the bottom of the device body 101, and the sterilization In the opposite position of the heater 141, a 1Kw-class warming heater 142 is installed to continuously maintain the raw water inoculated with the medium at a temperature that microorganisms like.

The cooling means 160 is for cooling the raw water heated by the sterilization heater 141 to a temperature suitable for inoculation of the medium (about 35° C.), and a cooling jacket 161 kept airtight in the lower cap 103 Is installed, and a cooling coil 162 is installed inside the cooling jacket 161 to be controlled by the controller 115.

The stirring means 180 is provided with a stirring shaft 181 lowered from the center of the upper cap 102 to the lower position of the device body 101, and the stirring shaft 181 is mounted on the upper cap 102. It is kept in the shaft housing 182.

The stirring shaft 181 is equipped with a stirring motor 185 having a gearbox 183 integrally so that the stirring shaft 181 can be rotated, and the stirring shaft 181 located inside the device body 101 A plurality of stirring impellers (186) are installed to be configured to stir evenly.

The light transmitting means 200 protrudes a circular or rectangular flange 201 from both sides of the upper cap 102 and the rear surface 104 and both side surfaces 106 of the device body 101, and to the flange 201 In order to prevent the leakage of microorganisms, the transparent transparent plate 205 with the airtight material 202 interposed therebetween is in close contact with each other and then fixed with an intermittent ring 206.

An LED-type floodlight 207 is installed on the floodlight panel 205 to change the light to be lit according to the type of microorganisms to be cultured by the controller 115, and to increase the cultivation performance by varying the illuminance. To be organized.

The present invention illustrates that one of the floodlights 207 is disposed in the center of the floodlight panel 205, but several small floodlights 207 may be disposed, and the same size as the floodlight panel 205 It will be possible to perform variously, such as arranging the transparent light 207 of.

A process of culturing microorganisms using the microorganism culturing apparatus to which the technology of the present invention is applied as described above is as follows.

First, if raw water is supplied to the inside of the device body 101 through the water supply pipe 123 by operating the water supply pump 122, the water supply is stopped after the proper amount is filled by the water level sensor, and after the water supply is supplied, the sterilization heater 141 is It is operated to heat the raw water to a temperature of 80 to 90℃, which is a temperature that does not boil, to kill common bacteria.

In this state, after replenishing the cooling water through the cooling water line 127, the cooling coil 162 inside the cooling jacket 161 installed on the lower cap 103 is operated to cultivate microorganisms and increase the raw water of a higher temperature together with the cooling water. Cooling is carried out to a temperature suitable for inoculation (temperature around 35° C.).

When the cooling of the raw water is completed, the inoculation manhole 108 provided in the upper cap 102 is opened to inoculate the medium, and then the viscous manhole 108 is closed, and the stirring motor 185 constituting the stirring means 180 is operated. In order to achieve even mixing of the medium and raw water, aeration, heating, and light transmission are performed to maintain conditions suitable for cultivation of microorganisms.

The stirring motor 185 rotates the stirring shaft 181 installed at the center of the internal axis of the device body 101, and the stirring impeller 186 provided in the stirring shaft 181 plays a role of stirring. The culture medium that was stirred by the stirring impeller 186 hits the corner surface 107 constituting the equipment body 101, and more evenly agitated and mixed.

In the case of aeration, the air is supplied by the air pump 124 through the air pipe 125 lowered from the upper cap 102 to the inside of the device body 101 as for supply of dissolved oxygen required for cultivation of the organism. , It will be natural to pass through the filter body 128 to prevent the inflow of foreign substances.

Of course, even when raw water is supplied, it will be natural that foreign matters are filtered between the feed pump 122 and the feed pipe 123 and the filter body 128 provided in the cooling water line 127 is filtered. Gas that may occur during the process is discharged through the discharge pipe 126 to ensure safety.

Maintaining the heating is to operate the heating heater 142 installed inside the device body 101 opposite the sterilization heater 141 to gradually raise the temperature to a suitable temperature for the cultivation of microorganisms without increasing the temperature rapidly. Is done.

In addition, in the case of microorganisms requiring photosynthesis by operating the floodlight 207 provided on the floodlight plate 105 installed on the upper cap 102 and the rear surface 105 of the device body 101 and both sides 106 Turn it on so that the culture can be performed smoothly.

The cultivation of the microorganisms usually takes about 5 days (5 days and 4 nights) based on 500 liters. During this period, the microorganisms are cultivated in an optimal state through continuous stirring, aeration, heating, and light transmission. , This may be manually manipulated by the user, but it will be natural that the controller 115 can be automatically performed from the start to the end according to the type of microorganism to be cultured.

When the cultivation of microorganisms is completed as described above, microorganisms are extracted and used through the drain valve 110, and the inside of the device body 101 through the cleaning manhole 109 installed on the front side 104 of the jangji body 101 It is enough to clean the microbial debris on the soil so that it does not interfere with the next microbial culture.

By repeating the above operation, it is possible to cultivate and use microorganisms necessary for crops, farming, and other purposes, and it is possible to cultivate various microorganisms in a small amount, and installation and maintenance costs are reduced without requiring a lot of installation space. It has the advantage of being able to minimize it.

100; Microbial culture device 101; Device body
102; Upper cap 103; Lower cap
108; Inoculation manhole 109; Cleaning manhole
110; Drain valve 115; Controller
120; Supply means 123; feed pipe
125; Air pipe 126; discharge pipe
127; Cooling water line 128; Filter body
140; Heating means 141; Sterilization heater
142; Heating heater 160; Cooling means
161; Cooling jacket 162; Cooling coil
180; Stirring means 181; Stirring shaft
185; Agitation motor 186; Stirring impeller
200; Light transmitting means 201; flange
205; Floodlight 207; Floodlight

Claims (6)

The device body 101 having a high height compared to the width of a polygonal body by connecting the rectangular corners having the front 104, the rear 105, and both sides 106 with a corner surface 107 having a length shorter than the length of each side. )Wow;
An upper cap 102 and a lower cap 103 protruding upwardly and downwardly protruding from the upper and lower portions of the device body 101;
An inoculation manhole 108 installed to inoculate a medium of microorganisms to be cultured in the front portion of the upper cap 102;
A cleaning manhole 109 installed in the central portion of the front 104 to clean the interior;
A drain valve 110 installed on the lower cap 103 to discharge the cultured microorganisms;
A supply means (120) installed in the device body (101) for supplying raw water and oxygen required for cultivation of microorganisms;
A heating means 140 for heating and sterilizing the raw water supplied by the supply means 120;
Cooling means 160 for cooling the sterilized raw water to a temperature suitable for inoculation of the medium;
A stirring means 180 for evenly mixing raw water and medium to cultivate microorganisms;
Transmitting means 200 to enable photosynthesis during microbial culture;
Includes a controller 115 mounted on one side of the device body 101 to control the supply means 120, the heating means 140, the cooling means 160, the stirring means 180, and the light transmitting means 200. Microbial culture device, characterized in that. The method of claim 1;
The supply means 120 includes a water supply pump 122 installed to supply raw water to the supply plate 121 protruding to the rear of the upper cap 102;
A water supply pipe 123 connecting the water supply pump 122 and the device body 101 to the inside;
An air pipe 125 connected to an air pump 124 installed on the side of the feed water pump 122 and positioned inside the device body 101 to supply oxygen required for microbial culture;
A discharge pipe 126 connected to discharge air bubbles or gases generated during the microbial culture process to the outside;
A cooling water line 127 installed to supply cooling water to the device body 101;
Microbial culture apparatus comprising a filter body (128) mounted on the water supply pipe (123), air pipe (125), exhaust pipe (125) and cooling water line (127) to filter foreign matter. The method of claim 1;
The heating means 140 includes a sterilization heater 141 of 10Kw or higher that is installed near the bottom of the device body 101 and heats the supplied raw water to kill common germs;
Microbial culture apparatus, characterized in that it comprises a 1Kw-class warming heater (142) to be installed in the opposite position of the sterilization heater (141) to continuously maintain the raw water inoculated with the medium at a temperature that the microorganism likes. The method of claim 1;
The cooling means 160 may include a cooling jacket 161 that maintains airtightness on the lower cap 103 so as to cool the raw water heated by the sterilization heater 141 to a temperature suitable for inoculation of the medium;
Microbial cultivation apparatus comprising a cooling coil (162) installed inside the cooling jacket (161) to cool the raw water. The method of claim 1;
The stirring means 180 includes a stirring shaft 181 installed down from the center of the upper cap 102 to a lower position of the device body 101;
A shaft housing 182 mounted on the upper cap 102 to hold the stirring shaft 181;
A stirring motor 185 for rotating the stirring shaft 181 by integrally mounting the gearbox 183 on the upper part of the shaft housing 182;
Microbial cultivation apparatus comprising a plurality of stirring impellers (186) that are mounted on a stirring shaft (181) located inside the apparatus body (101) to evenly stir the cultured microorganisms. The method of claim 1;
The light transmitting means 200 includes a flange 201 protruding from both sides of the upper cap 102 and the rear surface 104 and both side surfaces 106 of the device body 101;
A transparent light-transmitting plate 205 which is in close contact with the flange 201 by interposing an airtight material 202 on both sides to prevent leakage of microorganisms;
An intermittent ring 206 for controlling the flange 201 and the transparent plate 205;
Microbial cultivation apparatus, characterized in that it comprises a floodlight (207) installed on the floodlight panel (205) and turned on according to the type of microorganism to be cultured by the controller (115).

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