KR101828325B1 - Microorganism culture apparatus having improved culture efficiency - Google Patents

Abstract

The present invention relates to a microbial incubator with enhanced incubation efficiency. More specifically, the present invention relates to a microbial incubator with enhanced incubation efficiency, which enhances light penetration efficiency in an incubation tank by preventing deposition of organic substances or microbes on a surface of the incubation tank while incubating microbes in the incubation tank. By increasing activity or flow of the organic substances and microbes as well as contact frequency with microbes, it is possible to maximize incubation duration, rate of success in the incubation, and microbial incubation efficiency, thereby enabling incubation of high quality microbes in bulk within a short period.

Description

[0001] The present invention relates to a microorganism culture apparatus having improved culture efficiency,

The present invention relates to a microorganism culture apparatus with improved culture efficiency, and more particularly, to a microorganism culture apparatus which is capable of inhibiting the deposition of microorganisms and organic substances on the surface of a culture tank in culturing microorganisms in a culture tank, To a microorganism culturing apparatus having improved culture efficiency.

Recently, according to the cultivation of organic farming and national policies, various microorganisms are used for agriculture, animal husbandry and fisheries.

In the field of agriculture, selected microorganisms are cultivated, diluted in water, and then sprayed on soil to promote the production of crops to prevent insect pests.

In the livestock and fisheries sectors, cultured microorganisms are fed to livestock and fish or sprayed on water to promote the growth of livestock and fishes and to prevent disease, thereby enabling the production of livestock and fishes of high quality meat quality .

These microorganisms are required to be cultured for a long period of time by using a certain nutrient, temperature and light, and it is practically difficult to satisfy conditions for culturing microorganisms in a general farmhouse for a long time.

Accordingly, in this field, a variety of microbial culturing apparatuses have been developed and distributed to easily and stably utilize microbes. For example, Korean Patent Laid-Open Publication No. 2011-0134044 entitled " Stirring apparatus for microbial culture "has been proposed.

Such a stirring apparatus for microorganism culture includes a culture tank 100 in which a mixture (culture liquid) of a culture medium and a seed culture (hereinafter referred to as "microorganism") is stored as shown in FIG. 7, (300) arranged in the culture tank (100) and rotating to stir the mixture, a motor (300) for transmitting the power to the stirring means (200), and an air A stationary plate 130 is provided around the inner wall surface of the culture tank 100 to enhance mixing and stirring efficiency by colliding with a mixture flowing from the action of the stirring means 200.

However, such a stirring apparatus for culturing microorganisms has the following problems.

First, the stirring device for culturing the microorganisms has a problem that the growth rate of the microorganisms is slow according to the particle size of the mixture and the air introduced into the culture tank, so that a long incubation time is required.

Second, as each blade rotates and revolves through the driving of the motor, excessive resistance is generated in the rotation driving process of the blade, thereby causing damage to the motor and increasing the power consumption of the motor.

Third, since the culture tank is not provided with a sufficient amount of light due to the cultivation of the microorganism, the culture efficiency and the culture success rate of the microorganism deteriorate.

Fourthly, since the culture tank is configured to circulate the culture liquid by stirring the culture medium depending on the stirring means, a large amount of microorganisms are deposited on the inner wall of the culture tank and the temperature difference between the upper and lower portions of the culture tank is caused, The growth of the microorganisms deposited on the inner wall of the tank is reduced and the microorganisms are killed. On the other hand, it is difficult to culture the microorganisms of the uniform quality.

Thus, it is difficult to cultivate high-quality microorganisms in a large amount in a short time through the conventional microorganism culture apparatus. Therefore, the microorganism preparation prepared through the method is commercially available at a high price. Especially, since the culture time is long, It is practically difficult to produce a microorganism preparation or the like.

KR 10-1287757 B1 KR 10-2014-0042812 A KR 10-1433351 B1 KR 2011-0134044A KR 10-0660376 B1

SUMMARY OF THE INVENTION It is an object of the present invention, which is devised to overcome the above-mentioned problems, to provide a method of controlling microorganisms and organic matters deposited on a surface of a culture tank by culturing the microorganisms in a culture tank, And to increase the activity of the microorganism to increase the contact frequency between the microorganisms and the organic matter to maximize the microbial culture efficiency, the culture success rate, and the culture time, thereby culturing the microorganisms in a large amount in a short period of time.

The above object is achieved by the following constitutions provided in the present invention.

A microorganism culture apparatus improved in culture efficiency according to the present invention comprises:

A culture tank for storing and cultivating a culture medium containing the microbial agent;

And a circulation pump unit for internally circulating the culture liquid stored in the culture tank,

The circulation pump unit includes:

A suction pipe disposed at one upper half of the culture tank for providing a suction path for the stored culture liquid;

A water supply pipe communicating with a water supply port formed at the bottom of the culture tank; And
And a circulation pump arranged between the suction pipe and the water supply pipe for sucking the culture liquid formed in the upper part of the culture tank through a suction pipe and then discharging upward the culture liquid sucked into the bottom of the culture tank through a water supply pipe,
A bottom of the culture tank through which the culture liquid is discharged through the water supply pipe is provided with a rotation base arranged in a rotating structure on the bottom of the culture tank and a plurality of wings arranged in a radiation structure on the bottom surface of the rotation base, A diffusing pan for diffusing the culture liquid discharged through the water supply pipe in a one direction by interference with the discharged culture liquid is arranged,
And an elastic support member composed of a coil spring is disposed between the diffusion fan and the water supply pipe and the diffusion fan rotating upward while shielding the culture liquid upwardly discharged from the bottom of the culture tank in the water supply pipe due to the elastic refraction of the elastic support member, And irregularly diffusing the culture liquid upwardly discharged outward while swinging,
Wherein a hollow sprouting forest comprising a funnel-shaped hollow body supported by traction springs is disposed on a sidewall of the culture tank, and an axial pipe diffusion section is formed between the hollow sprouting forest and the sidewall, do.
Preferably, the culture tank has a funnel-shaped lower part, and a light transmitting window is formed on the side wall. On the outer side of the culture tank, lighting members for irradiating light into the culture tank through a light transmitting window are disposed.

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More preferably, in the bottom of the incubation tank, there is provided a hot water tank having a multi-layer structure and storing hot water, and an electrothermal heater disposed in the hot water tank to provide heat to the hot water disposed in the hot water tank A heating portion is formed,

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The hot water stored in the hot water tank is heated through the electric heater so that the medium stored in the incubator is heated by the hot water radiating from the hot water.

In addition, the hot water tank is provided with a steam supply pipe for injecting high-temperature steam, which is evaporated at a high temperature into the hot water stored in the hot water tank through the electrothermal heater, into the culture tank, and the high temperature steam is injected into the culture tank through the steam supply pipe And sterilize the culture tank after culturing the microorganism.

As described above, in the present invention, in order to increase the frequency of contact between nutrients and air required for the growth of microorganisms and microorganisms by circulating the culture liquid stored in the culture tank through the circulation pump unit, and to prevent precipitation of organic matter and microorganisms , And an induction diffusion structure for diffusing the culture liquid upwardly discharged from the bottom to the outside is added to the bottom of the culture tank.

Therefore, the culture liquid forms an ascending water flow moving upward along the inner wall of the culture tank, and the deposition of microorganisms and organic matter on the side wall of the culture tank is suppressed by the ascending water flow. Therefore, the light transmittance Or the phenomenon that the flow of organic matter and microorganisms is lowered can be suppressed.

Particularly, the diffusion fan forming the induction diffusion structure not only has a simple structure, but also stably diffuses the culture liquid. Particularly, the culture liquid diffused by the hollow propagation forest is collected and the flow rate is increased. It is possible to stably move up to the upper layer along the inner wall.

Thus, the present invention suppresses the deposition of organic matter or microorganisms on the side wall of the culture tank without any scraping process through a scraper device, and as a result, light stably enters the culture tank, and microorganisms and organic matter flow stably , And consequently it is possible to cultivate a large amount of microorganisms in a short time.

1 and 2 are an exploded perspective view and a cross-sectional view showing the overall structure of a microorganism culture apparatus with improved culture efficiency proposed as a preferred embodiment of the present invention,
FIG. 3 is an enlarged view of the 'A' portion in FIG. 1,
FIG. 4 and FIG. 5 are graphs showing the state of cultivation of microorganisms through a microorganism culture apparatus with improved culture efficiency, which is proposed as a preferred embodiment of the present invention,
FIG. 6 is an operational state diagram showing a steam sterilization process in a culture tank through a microorganism culture apparatus with improved culture efficiency proposed as a preferred embodiment of the present invention,
7 shows a conventional microorganism culture apparatus.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, a preferred embodiment of the present invention will be described in detail with reference to the accompanying drawings.

FIGS. 1 and 2 are an exploded perspective view and a cross-sectional view showing the entire configuration of a microorganism culture apparatus with improved culture efficiency proposed as a preferred embodiment of the present invention. FIG. 3 is a cross- FIGS. 4 and 5 are graphs showing the state of cultivation of a microorganism through a microorganism culture apparatus with improved culture efficiency, which is proposed as a preferred embodiment of the present invention. FIG. FIG. 2 is a view showing an operation of steam sterilization in a culture tank through a microorganism culture apparatus with improved culture efficiency.

The microorganism culturing apparatus (1) with improved culture efficiency proposed in the present invention is a microorganism culture apparatus in which a culture medium containing a microorganism preparation is stored in a culture tank in which temperature, light source, nutrition, And cultivate a large amount of microorganisms.

As shown in FIGS. 1 and 2, the microorganism culture apparatus 1 includes a culture tank 10 for storing and cultivating a culture medium containing a microorganism preparation; And a circulation pump unit 20 for internally circulating the culture liquid stored in the culture tank 10.

The thus constructed microorganism culture apparatus 1 continuously circulates the culture liquid stored in the culture tank 10 through the circulation pump unit 20 to suppress the sedimentation of organic substances or microorganisms contained in the culture liquid to the bottom In addition, microorganisms and organic matter, which are food of microorganisms, are in constant contact with each other, so that microorganisms can cultivate vigorously through stable intake of organic matter.

According to the present embodiment, the culture tank 10 has a funnel shape at the bottom and a light transmitting window 13 is formed at the side wall 10a. The microorganism, such as photosynthetic bacteria, So that it is possible to supply stable light required for culturing of the cells.

Lighting members 14 for irradiating light into the culture tank 10 through a light transmitting window 13 are disposed on the outside of the culture tank 10 so as to be required to culture the microorganisms in the culture tank 10 So that the light is stably provided.

The circulation pump unit 20 includes a suction pipe 22 communicating with the circulation drain 12 formed at one point of the upper half of the culture tank 10 and providing a suction path for the stored culture liquid; A water supply pipe (21) arranged upright on a water supply port (11) formed at a lower portion of the culture tank; And a suction pipe 22 disposed between the suction pipe 22 and the water pipe 21 to suck the culture liquid in the upper portion of the culture tank 10 through the suction pipe 22 and then sucked into the bottom 10b of the culture tank through the water pipe 21. [ And a circulation pump 23 for upwardly discharging the culture liquid.

The water supply port 11 formed at the bottom of the culture tank 10 is used as a supply path for the culture liquid to be supplied through the circulation pump unit 20 and is also used as a microbial culture liquid which has been cultured in the culture tank 10 It is also used as a discharge path to the outside.

In the present embodiment, the air supply tube 61 of the air bladder 60 is connected to the water supply pipe 21 of the circulation pump unit 20, and a water supply pipe (not shown) is connected to the bottom 10b of the culture tank 10 21 and the air supplied through the air supply pipe 61 are mixed together to be discharged upward.

In this embodiment, a primary filtration filter 62 is provided at the inlet of the air bladder 60 and a secondary filtration filter 63 is provided on the air supply tube 61. The water supply pipe 21 The air that has been multi-filtered sequentially is supplied by the primary filtration filter 62 and the secondary filtration filter 63.

At this time, the secondary filtration filter 63 disposed on the air supply pipe 61 maintains a relatively higher phase than the water level of the culture solution stored in the culture tank 10, (61).

The spiral screw 24a is disposed in the water supply pipe 21 and an agitating chamber 24 having a spiral guide path 24b is disposed. The spiral screw 24a is provided with an anode S polarity) of the permanent magnets 25 are disposed.

The organic particles and the air particles contained in the culture liquid passing through the spiral guide path 24b of the stirring chamber 24 pass through the spiral guide path 24b and are discharged from the permanent magnet 25 (S polarity or N polarity) due to the magnetic polarity of the magnetic force of N polarity or S polarity.

That is, when the permanent magnet 25 is disposed in the stirring chamber 24 so as to expose the N polarity, the organic particles or air particles passing through the stirring chamber 24 have S polarity, and the organic substance The particles or air particles are mutually repulsive by the magnetic polarity (S polarity or N polarity) of the other pole.

Thus, the organic particles or air particles that are mixed into the culture liquid and used as food for the microorganisms flow and disperse in the culture tank due to the magnetic polarity (S polarity or N polarity) of the other electrode. As a result, The frequency of contact of the microorganisms is increased and the stable intake of food and air is possible.

4 to 5 and FIG. 5, in the case of upwardly discharging the culture liquid aspirated from the upper portion of the culture tank 10 through the circulation pump unit 20 through the bottom 10b of the culture tank 10, The culture liquid discharged upward from the bottom 10b of the culture tank 10 is diffused outward and is moved upward along the side wall 10a of the culture tank 10. [

With this configuration, the phenomenon that organic matter and microorganisms are deposited on the sidewall of the culture tank 10 by the water flow formed by the culture liquid moving along the side wall of the culture tank 10 is suppressed.

As a result, as organic matter and microorganisms are deposited on the sidewall of the culture tank 10, the light transmission efficiency is lowered in the culture tank 10, and the fluidity of the microorganisms and the organic matter is lowered, The phenomenon that the contact frequency of the microorganism and the organic matter that is the food of the microorganism is reduced can be suppressed.

To this end, in this embodiment, as shown in FIGS. 1 to 5, the culture liquid discharged through the water supply pipe 21 is diffused outward to the bottom 10b of the culture tank 10 through which the culture liquid is discharged through the water supply pipe 21 The culture liquid which is discharged upward in the culture tank 10 through the water supply pipe 21 by the diffusion fan 30 is diffused to the outside so that the side wall of the culture tank 10 Thereby forming a rising water stream.

3, a plurality of vanes 32 are arranged in a radial pattern on the bottom surface of a rotating base 31 disposed in a lower portion of the culture tank 10 in a rotating structure, , And flows in one direction while shielding the upward flow of the culture liquid upwardly through the water supply pipe (21) to diffuse the shielded culture liquid outward.

An elastic support member 34 made of a coil spring is disposed between the diffusion fan 30 and the water supply pipe 21 so that the elastic support member 34 is elastically bent as shown in FIG. The diffusion fan 30 rotating upward while shielding the culture liquid upwardly discharged from the bottom 10b of the culture tank 10 elastically moves up and down to diffuse irregularly outwardly the culture liquid discharged upward.

The culture liquid discharged upward through the water supply pipe 21 of the circulation pump unit 20 flows into the culture tank 10 by the interference and rotation with the diffusion fan 30 disposed at the upper portion of the water supply pipe 21, The side wall 10a of the culture tank 10 is cleaned by the water flow formed by the culture liquid which moves upward along the side wall 10a of the culture tank 10, The deposition of microorganisms and organic matter on the sidewall 10a can be suppressed.

In this embodiment, the hollow forest 40 is disposed on the side wall 10a of the culture tank 10 and is formed along the side wall 10a of the culture tank 10 by the diffusion fan 30 The ascending water stream is moved upward by the hollow forest 40 to the upper portion of the culture tank 10.

According to the present embodiment, the hollow sprouting forest is formed of a funnel-shaped hollow body, and is formed between the outer wall 40a of the hollow sprouting forest 40 on the side wall 10a of the culture tank 10, And an axial tube diffusion section 41 in which the width gradually decreases from the lower part to the upper part is formed.

In the present embodiment, two hollow sprouting shafts 40 are disposed apart from the side walls 10a of the culture tank 10, and the hollow sprouting shafts 40 are formed on the side walls 10a of the culture tank 10 And is elastically lifted by the traction springs 42 in the radial direction so as to be elastically lifted by a change in the flow rate of the culture liquid moving upward along the side wall 10a of the culture tank 10, (10a) is guided to the side wall of the culture tank (10).

The culture liquid diffusing outward by the diffusion fan 30 and moving upward along the sidewall 10a of the culture tank 10 is then supplied to the outer wall of the hollow crevice forest 40 and the outer wall of the sidewall 10a The water collecting and flow velocity is increased and the water flows along the side wall 10a of the culture tank 10 at a high speed.

The culture liquid formed in the upper portion of the culture tank 10 is discharged upward from the bottom 10a of the culture tank 10 through the water supply pipe 21 of the circulation pump unit 20, And is collected through an axial pipe diffusion section 41 formed by the hollow sprouting forest 40 in this process and is collected in the side wall 10a of the culture tank 10, (10a).

As a result, a rising water flow layer is continuously formed on the side wall 10a of the culture tank 10 by the upwardly moving culture liquid. As a result, the rising water flow layer is formed on the side wall 10a of the culture tank 10, Or the deposition of microorganisms is suppressed.

In addition, the microorganisms stored in the culture tank 10 should be maintained in nutrition and air, sunlight, and proper temperature.

In this embodiment, the incubation tank 10 is provided with a hot-water heating unit 50. In the course of culturing the microorganisms, temperature is set at a predetermined temperature in the culture medium as shown in FIG. 4 to form temperature conditions suitable for culturing the microorganisms. After the microorganisms are cultured, a high temperature steam is supplied into the culture tank 10 as shown in FIG. 6 to perform a sterilizing function of sterilizing the germs remaining in the culture tank 10 through the high temperature steam.

The hot water heating unit 50 is basically a multi-layer structure formed on the bottom of the culture tank 10 and includes a hot water tank 51 storing hot water and a hot water tank 51 disposed in the hot water tank 51, And an electrothermal heater 52 for providing electric heat to the hot water disposed in the bath 51.

In the incubation process of the microorganisms, the electrothermal heater 52 is driven in accordance with the temperature value measured through a temperature sensor (not shown) disposed in the incubation tank and measuring the temperature of the culture liquid stored in the incubation tank. State.

A steam supply pipe 53 formed with a pressure valve 54 is formed between the culture tank 10 and the hot water tank 51. The hot water stored in the hot water tank 51 is heated to a predetermined temperature and evaporated When the inner pressure of the hot water tank 51 rises above the set pressure, the pressure valve 54 is opened and the high-pressure steam evaporated in the hot water tank 51 through the steam supply pipe 53 flows into the culture tank 10, Pressure discharge. In the culture tank, a steam injection nozzle conduit 55 communicating with the steam supply pipe 53 and having a plurality of steam injection nozzles 55a is formed.

In this embodiment, a plurality of hollow induction radiating rods 51a protruding into the culture tank 10 are formed in the hot water bath 51, and the heat generated in the hot water bath 51 is discharged into the hollow So that the culture liquid stored in the culture tank 10 is quickly and uniformly supplied through the induction radiating rods 51a.

4, the control unit controls the operation of the electrothermal heater 52 according to the temperature of the culture liquid measured through the temperature sensor (not shown) and the operation state thereof, Is heated to a set temperature, the culture liquid stored in the culture tank 10 is heated to a predetermined temperature.

6, the control unit heats and boils the hot water through the electrothermal heater 52 so that the steam is supplied to the steam supply pipe 53, and the micro- The high-temperature steam is discharged into the incubation tank 10 through the high-temperature steam generator 10 to sterilize the inside of the culture tank 10 by the discharged high-temperature steam.

Therefore, in the culturing process of the microorganism by the hot water heating unit 50, the culture liquid is heated at a predetermined temperature, so that a culture temperature condition suitable for culturing the microorganism is formed in the culture tank, so that the microorganism grows vigorously.

After completion of the cultivation of the microorganism, high-temperature steam is discharged into the culture tank 10 at a high pressure to sterilize the germs remaining in the culture tank 10 stably.

Particularly, in this embodiment, as shown in Fig. 6, an ejection orifice 51b having a pressure valve is formed in a hollow heat radiating rod 51a for radiating the heat generated in the hot water tank heating unit 50 to the culture liquid stored in the culture tank do.

According to this configuration, in the process of storing the culture liquid in the culture tank 10 and culturing the microorganisms, the ejection orifice 51b is shielded by the pressure valve and is uniformly diffused into the culture liquid through the hollow heat- However,

In the steam sterilization process, the vapor injection valve 51b is opened by the vapor pressure generated in the hot water tank 51 so that the steam supplied into the heat radiation guide rod 51a is dispersed and discharged more quickly into the culture tank 10.

Therefore, the present invention is capable of heating the culture liquid and discharging the steam at high pressure in the culture tank, respectively, so that it is possible to sterilize and wash the culture tank without providing a separate steam sterilizing device.

1. Microorganism culture apparatus with improved culture efficiency
10. Culture vessel
10a. Side wall 10b. floor
11. Water supply hole 12. Circulation drain hole
13. Flood light window 14. Lighting member
20. Circulation pump section 21. Water supply pipe
22. Suction tube 23. Circulation pump
24. Stirring chamber 24a. Spiral screw
24b. Spiral guide 25. Permanent magnet
30. Diffusion Fan 31. Rotating Base
32. Wings 33. Rotor
34. Elastic support member
40. Hollow Shrub 41. Shore Diffusion Section
42. Traction spring
50. Heated hot water heater 51. Heated hot water heater
51a. The heat dissipating rod 51b. Discharge nozzle
52. Electric heaters
53. Steam line 54. Pressure valve
55. Steam injection nozzle line 55a. Steam injection nozzle
60. Air Blur Warfare 61.
62. Primary Filtration Filter 63. Secondary Filtration Filter

Claims (5)

1. A microorganism culture apparatus comprising a culture tank for storing and culturing a culture solution containing a microorganism preparation and a circulation pump unit for circulating the culture solution stored in the culture tank,
The circulation pump unit includes:
A suction pipe disposed at one upper half of the culture tank for providing a suction path for the stored culture liquid;
A water supply pipe communicating with a water supply port formed at the bottom of the culture tank; And
And a circulation pump arranged between the suction pipe and the water supply pipe for sucking the culture liquid formed in the upper part of the culture tank through a suction pipe and then discharging upward the culture liquid sucked into the bottom of the culture tank through a water supply pipe,
A bottom of the culture tank through which the culture liquid is discharged through the water supply pipe is provided with a rotation base arranged in a rotating structure on the bottom of the culture tank and a plurality of wings arranged in a radiation structure on the bottom surface of the rotation base, A diffusing pan for diffusing the culture liquid discharged through the water supply pipe in a one direction by interference with the discharged culture liquid is arranged,
And an elastic support member composed of a coil spring is disposed between the diffusion fan and the water supply pipe and the diffusion fan rotating upward while shielding the culture liquid upwardly discharged from the bottom of the culture tank in the water supply pipe due to the elastic refraction of the elastic support member, And irregularly diffusing the culture liquid upwardly discharged outward while swinging,
Wherein a hollow sprouting forest comprising a funnel-shaped hollow body supported by traction springs is disposed on a sidewall of the culture tank, and an axial pipe diffusion section is formed between the hollow sprouting forest and the side wall, Wherein the microorganism is cultured in the culture medium. The culture apparatus according to claim 1, wherein the culture tank has a funnel-shaped lower part, and a light transmitting window is formed on the side wall, and illumination members for irradiating light into the culture tank through a light transmitting window are disposed outside the culture tank A microorganism culture apparatus with improved culture efficiency delete The heating apparatus according to any one of claims 1 to 3, further comprising: a bath heater formed in the bottom of the incubator in a multi-layered structure for storing bath water; and an electrothermal heater disposed in the bath heater for providing heat to the bath water disposed in the bath heater A hot water heater is formed,
Wherein the hot water stored in the hot water tank is heated through an electrothermal heater so as to heat the medium stored in the incubator through hot air discharged from the hot water. [5] The apparatus according to claim 4, wherein the hot water tank is provided with a steam supply pipe for injecting high temperature steam, which is evaporated from the hot water stored in the hot water tank, through the electric heater into the culture tank, Wherein the microorganism culture apparatus is configured to sterilize the culture tank after the culture of the microorganism has been completed.

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