KR100729393B1 - Cultivator for micro-organism medium soil - Google Patents

Cultivator for micro-organism medium soil Download PDF

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KR100729393B1
KR100729393B1 KR1020060036464A KR20060036464A KR100729393B1 KR 100729393 B1 KR100729393 B1 KR 100729393B1 KR 1020060036464 A KR1020060036464 A KR 1020060036464A KR 20060036464 A KR20060036464 A KR 20060036464A KR 100729393 B1 KR100729393 B1 KR 100729393B1
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culture
culture medium
culture tank
opening
pipe
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KR1020060036464A
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Korean (ko)
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김형준
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김형준
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
    • C12M1/06—Apparatus for enzymology or microbiology with gas introduction means with agitator, e.g. impeller
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

Abstract

A cultivator for soil microorganisms is provided to improve safety of microorganism cultivation by inhibiting contamination and damage of microorganisms caused by a stirring fan and an electric heater located within a culture vessel. The cultivator for soil microorganisms comprises: a cylindrical stainless steel culture vessel(1) mounted to a certain position by supporting legs(1a), and having an opening part(1c) with a microorganism inlet(1b) and a cover(1d); a culture medium-heating device(2) located outside of the culture vessel(1) and comprising a heating bucket(20), a porous bucket contained in the heating bucket and an electric heater(22) for heating the culture medium contained in the porous bucket; a circulation device(4) mounted under the culture vessel, and containing a water pump for absorbing the culture medium and transferring it into the heater; a stirring device connected to a supply pipe for supplying the culture vessel from the culture medium-heating device into the culture vessel, and having two outlets in both ends of a connection pipe and an opening/closing valve between the outlets; a projector containing a projecting plate in one side of the main wall of the culture vessel and a lighting chamber outside of the projecting plate for projecting a light into the culture vessel from the lighting chamber; and an electrical control device(7) having a lighting device with a UV light and temperature sensor(3).

Description

토양미생물 배양기{cultivator for micro-organism medium soil}Cultivator for micro-organism medium soil

도 1은 본 발명의 외관 사시도1 is an external perspective view of the present invention

도 2는 본 발명의 단면 예시도2 is a cross-sectional view of the present invention

도 3은 본 발명의 배양액 가열기 단면도Figure 3 is a cross-sectional view of the culture medium heater of the present invention

도 4는 본 발명의 교반기 평면도4 is a plan view of the agitator of the present invention

도 5는 본 발명의 교반기 토출구 사시도Figure 5 is a perspective view of the stirrer discharge port of the present invention

도 6은 본 발명의 배양조 단면도6 is a cross-sectional view of the culture tank of the present invention

도 7은 본 발명의 전기콘트롤 장치의 외관예시도7 is an external view of the electric control device of the present invention

도 8은 본 발명의 배양조 횡단면도8 is a cross-sectional view of the culture tank of the present invention

도 9는 본 발명의 배면예시도이다.9 is a rear view of the present invention.

* 도면의 주요부분에 대한 부호설명* Explanation of symbols on the main parts of the drawings

1 : 배양조 1b : 투입구1: culture tank 1b: inlet

1c : 개방부 1d : 뚜껑1c: opening 1d: lid

2 : 배양액 가열기 3 : 온도감지센서2: culture medium heater 3: temperature sensor

4 : 순환장치 5 : 교반기4 circulator 5 agitator

6 : 투광기 7 : 전기컨트롤장치6: floodlight 7: electric control device

8 : 자외선멸균등 10 : 배출관 10a : 중간연결부 20 : 가열원통 21 : 다공망통 21a : 노출부 22 : 전기히터 23 : 공급관 24 : 보충수공급통 40 : 장치판 41 : 양수기 41a : 흡입관 41b : 토출관 42 : 연결관 44 : 배출관 50 : 연결관 51,52 : 토출구 53 : 개폐밸브 B1,B2,B3,B4 : 개폐밸브DESCRIPTION OF SYMBOLS 10 Ultraviolet sterilization lamp 10 Discharge pipe 10a Intermediate connection part 20 Heating cylinder 21 Porous cylinder 21a Exposure part 22 Electric heater 23 Supply pipe 24 Supplementary water supply container 40 Device plate 41 Water pump 41a Intake pipe 41b Discharge Pipe 42: Connection pipe 44: Outlet pipe 50: Connection pipe 51, 52: Outlet port 53: On-off valve B1, B2, B3, B4: On-off valve

본 발명은 토양미생물을 편리하게 배양시킬 수 있는 배양기에 관한 것인데 보다 구체적인 것은 스텐레스스틸제로 되어 지지각에 장치되는 원통형의 배양조와 배양조 외부에서 배양액을 전기히터로 가열하여 배양조로 공급할 수 있도록 장치한 배양액 가열기와 배양조 하측에 장치되어 배양액을 흡인하여 배양액 가열기로 공급하고 배양액가열기에서 다시 배양조로 공급될 수 있게 양수기를 사용하여 구성시킨 순환장치와 배양액가열기에서 배양조로 공급되는 공급관에 설치하여 공급되는 압력으로 배양액을 순환시키는 교반기와 배양조 주벽에 장치되어 투광조의 투광벽을 통해 배양조내에 빛을 공급하는 투광기와 자외선 멸균등으로 이루어진 조명부와 온도감지센서로 이루어지고 이들을 제어하는 전기콘트롤장치를 갖추어서 토양미생물을 편리하게 배양시킬 수 있게 한 것이다.The present invention relates to an incubator capable of cultivating soil microorganisms in a convenient manner, and more specifically, a device for supplying a culture medium by heating with an electric heater from a cylindrical culture tank and a culture tank installed at a support angle made of stainless steel. Installed in the culture medium heater and the lower side of the culture tank, the culture medium is aspirated and supplied to the culture medium heater, and is installed and supplied to the circulator configured by using a water pump so that the culture medium heater can be supplied to the culture tank and the supply pipe supplied from the culture medium heater to the culture tank. Equipped with a stirrer to circulate the culture medium under pressure and the main wall of the culture tank to supply light to the culture tank through the floodlight of the floodlight, and an illumination unit consisting of ultraviolet sterilization lamp and an electric control device to control them. Convenient soil microorganisms It is to be cultured.

종래의 미생물 배양기는 배양조내부에 직접 전기히터를 장치하고 교반팬을 장치하여 이를 전동기에 연결하여 교반시킴으로써 배양액을 직접 전기히터로 가열하고 교반시키고 있으나 교반팬 연결부위의 부식과 과잉저항으로 배양중의 미생물이 오염되고 손상되는 문제가 있고 배양조내에 전기히터를 장치하여 배양액을 가열하기 때문에 전기히터에 접촉하는 일부 미생물이 사멸되는 등의 결점이 있어서 안전성이 떨어지는 결점이 있었다.Conventional microbial incubator is equipped with an electric heater directly inside the culture tank and the stirring fan is connected to an electric motor to agitate the culture medium by direct heating and stirring, but the culture medium is incubated due to corrosion and excess resistance of the stirring pan connection Of microorganisms are contaminated and damaged, and because of the heating of the culture medium by installing an electric heater in the culture tank, some microorganisms that come into contact with the electric heater are killed, and thus there is a disadvantage that the safety is low.

본 발명은 상기와 같은 종래의 결점을 개선하여 농민이 편리하게 사용할 수 있고 배양효과를 높일 수 있는 안전한 배양기를 공급하여 쉽게 토양미생물을 배양시켜 사용함으로써 친환경 농법의 정착을 앞당겨 국토보전 및 농가 소득 증대에 이바지할 수 있게 한 것이다.The present invention provides a safe incubator that can be conveniently used by farmers to improve the conventional defects as described above, and by easily cultivating soil microorganisms to facilitate the establishment of eco-friendly farming methods to increase land conservation and farm income. To help.

이를 보다 상세히 첨부된 도면에 의거 설명하면 다음과 같다.This will be described based on the attached drawings in more detail.

본 발명은 스텐레스스틸로 제작되어 지지각(1a)에 의하여 일정위치에 장치되고 균주투입구(1b)와 뚜껑(1d)이 있는 개방부(1c)를 구비한 배양조(1)와 배양조 외부에 장치되어 전기히터(22)로 배양액을 가열하여 배양조내에 공급하는 배양액가열기(2)와 배양조 하측에 장치되어 배양액을 흡인하여 이를 배양액 가열기로 공급하고 배양액가열기에서 다시 배양조로 공급하여 순환이 이루어지게 하는 양수기가 구비된 순환장치(4)와 배양액가열기에서 배양조로 공급되는 공급관에 장치되어 배양액이 공급되는 압력으로 배양액을 교반할 수 있게 만든 교반기(5)와 배양조 주벽에 투광판(1e)이 장치되어 그 외면에 조명실(1f)이 형성되고 조명실내에 배양조로 향해 조명되도록 투광기(6)와 자외선멸균등(8)이 장치되어 배양조내에 빛을 공급하거나 배양조 내부에 자외선을 공급하는 조명장치(1g)와 배양조 내부에 장치되는 자외선멸균등(8)과 배양조 상단부에 장치되어서 배양조내의 온도를 감지하는 온도감지센서(3)와 이들을 필요에 따라 제어하고 운용하는 전기콘트롤장치(7)로 이루어진다.The present invention is made of stainless steel and installed in a predetermined position by the support angle (1a) and having a strain inlet (1b) and the opening (1c) having a lid (1d) and the outside of the culture tank (1) It is installed in the culture medium heater (2) and the lower side of the culture tank is heated to supply the culture medium with the electric heater 22 and the culture medium is aspirated and supplied to the culture medium heater and fed back to the culture tank from the culture medium heater and the circulation is made A circulator 4 equipped with a water pump, and a stirrer 5 installed in a feed tube supplied to the culture tank from the culture medium heater to agitate the culture medium at a pressure supplied with the culture medium and a floodlight plate 1e on the culture vessel main wall. This apparatus is provided with an illumination chamber 1f on the outer surface thereof, and a light emitter 6 and an ultraviolet sterilization lamp 8 are provided to illuminate the culture chamber toward the culture vessel so as to supply light into the culture vessel or ultraviolet rays inside the culture vessel. It is equipped with an illumination device (1g) for supplying the light and the UV sterilization lamp (8) installed inside the culture tank and the temperature sensing sensor (3) to detect the temperature in the culture tank and to control and operate them as necessary It consists of an electric control device (7).

따라서 본 발명의 배양액가열기(2)는 가열원통(20) 내부에 다공망통(21)이 장치되어 그 속에 전기히터(22)가 장치되고 다공망통(21)의 상단 일부가 가열원통(20) 상부로 노출되어 노출부(21a)에 보충수공급통(24)이 장치되어 보충수를 공급할 수 있도록 되며 가열원통(20) 상부에서 배양조로 공급관(23)이 형성되어 전기히터와 간접접촉으로 가열된 배양액이 가열원통에서 배양조(1)로 공급되며 Therefore, the culture medium heater (2) of the present invention is equipped with a porous network 21 inside the heating cylinder 20, the electric heater 22 is installed therein, and a part of the upper end of the porous network 21 is the heating cylinder 20 upper portion Exposed to the exposed portion (21a) is provided with a supplemental water supply cylinder 24 to supply the supplemental water and the supply pipe 23 is formed in the culture tank in the heating cylinder 20 is heated in indirect contact with the electric heater The culture solution is supplied from the heating cylinder to the culture tank (1)

본 발명의 순환장치(4)는 배양조 하측에서 지지각에 고정한 장치판(40)위에 양수기(41)가 장치되어 그 흡입관(41a)에 배양조(1) 하단에서 외부로 형성되는 배 출관(10)의 중간연결부(10a)와 연결관(42)으로 연결되어 중간연결부에 개폐밸브(B2)가 장치되고 배출관(10) 끝에 개폐밸브(B1)가 장치되며 양수기(41)의 토출관(41b)은 가열원통(20)의 하단 유입구(20a)와 연결관(43)으로 연결되어 가열원통 입구에 개폐밸브(B3)가 장치되고 그 하측에서 외부로 형성되게 배출관(44)을 더 부착하여 배출관(44)의 출구에 개폐밸브(B4)를 장치함으로써 배양액을 가열원통으로 순환시키거나 외부로 인출하여 용기(60)(61)에 수용시킬 수 있게 되고 교반기(5)는 배양액가열기(2)의 공급관(23)에 연결되도록 배양조 상면에 연결관(50)을 설치하여 그 입구와 출구측에 일정각도로된 토출구(51)(52)를 장치하고 입구측 토출구(51)와 출구측 토출구(52)가 파생되는 부분에 개폐밸브(53)를 설치하여서 연결관에서 토출구로 토출되는 배양액의 압력으로 배양액을 교반시킬 수 있게 구성하며 투광기(6)는 배양조의 투광판(1e)을 통해 태양빛 또는 황색 불빛을 배양조 내부에 공급할 수 있도록 하고 자외선멸균등(8)은 배양조내에 자외선을 공급하여 멸균작용을 하며 온도감지센서(3)는 배양조에 장치되어 공지의 방법으로 배양조의 배양액온도를 감지하여 전기히터 작동부에 신호를 줌으로써 배양액의 가열온도를 일정하게 유지할 수 있도록 된다.In the circulation device 4 of the present invention, a water pump 41 is provided on the device plate 40 fixed to the support angle at the lower side of the culture tank, and the discharge pipe formed at the suction pipe 41a from the lower side of the culture tank 1 to the outside ( 10 is connected to the connecting portion (10a) and the connecting pipe (42), the opening and closing valve (B2) is provided in the intermediate connecting portion, the opening and closing valve (B1) is provided at the end of the discharge pipe (10) and the discharge pipe (41b) of the water pump 41 ) Is connected to the lower inlet 20a of the heating cylinder 20 and the connection pipe 43 so that the opening / closing valve B3 is installed at the inlet of the heating cylinder, and the discharge pipe 44 is further attached so that the discharge pipe 44 is formed from the lower side to the outside. By opening / closing the valve B4 at the outlet of 44, the culture medium can be circulated to the heating cylinder or drawn out to be accommodated in the vessels 60 and 61, and the stirrer 5 of the culture solution heater 2 A connection pipe 50 is installed on the upper surface of the culture tank so as to be connected to the supply pipe 23, and the discharge ports 51 and 5 are formed at a predetermined angle on the inlet and outlet sides thereof. 2) and the opening / closing valve 53 at the inlet side outlet 51 and the outlet side outlet 52 are derived so that the culture medium can be stirred by the pressure of the culture medium discharged from the connecting pipe to the outlet. Transmitter 6 is to supply the sunlight or yellow light to the inside of the culture tank through the light-transmission plate (1e) of the culture tank, UV sterilization lamp (8) is sterilized by supplying ultraviolet light in the culture tank and the temperature sensor ( 3) is installed in the culture tank to detect the culture medium temperature of the culture tank by a known method to signal the electric heater operation unit to maintain the heating temperature of the culture solution.

이와 같이 된 본 발명은 배양조(1)의 개방부(1c)를 통해 배양용수(일반물)와 배지를 넣고 가열기(2)의 보충수공급부(24)까지 가득차도록 물을 부운후 밸브(B1)(B4)를 잠그고 밸브(B2)(B3)를 개방하여 교반기(5)의 개폐밸브(53)로 토출구 의 토출압을 조종하고 전기컨트롤박스의 전원 스윗치를 넣고 양수기(4)를 가동한 상태에서 자외선멸균등(8)을 점등시켜 10분간 배양용수 및 배지를 멸균시킨다.In the present invention thus obtained, the culture water (general substance) and the medium through the opening 1c of the culture tank 1, the water is poured to the replenishment water supply section 24 of the heater 2, the valve (B1) (B4) is closed and valves (B2) and (B3) are opened to control the discharge pressure of the outlet by the on / off valve (53) of the stirrer (5), the power switch of the electric control box is put in, and the water pump (4) is operated. Sterilize the culture water and medium for 10 minutes by turning on the UV sterilization lamp (8).

이때 교반기(5)의 토출구(51)(52)로 토출되는 수압과 토출각도에 의하여 배양용수는 시계방향으로 돌면서 교반된다.At this time, the culture water is stirred while turning clockwise by the water pressure and the discharge angle discharged to the discharge ports 51 and 52 of the stirrer 5.

10분간 경과되면 자외선멸균등(8)을 소등하고 균주투입구(1b)를 통해 배양하고자 하는 균주를 투입한다.After 10 minutes the UV sterilization lamp (8) is turned off and the strain to be cultured through the strain inlet (1b).

배양액 온도를 섭씨35도로 설정하고 전기히터를 작동시키면 가열원통(20)으로 공급된 배양액이 전기히터(22)가 장치된 다공망통(21)의 온수와 혼합되어 가열되고 이것이 배양조에 공급되어 일정온도의 배양액이 공급되고 온도센서(3)에 의하여 감지가 되며 배양액의 온도가 높거나 낮으면 온도센서가 이를 감지하여 전기히터(22)의 온도를 제어하여 배양액 온도를 일정하게 유지시킨다.When the temperature of the culture medium is set to 35 degrees Celsius and the electric heater is operated, the culture solution supplied to the heating cylinder 20 is mixed with the hot water of the porous network 21 equipped with the electric heater 22 and heated, and this is supplied to the culture tank to maintain a constant temperature. The culture medium is supplied and is detected by the temperature sensor (3), if the temperature of the culture medium is high or low, the temperature sensor detects this to control the temperature of the electric heater 22 to maintain a constant culture temperature.

배양시간은 컨트롤장치의 타이머에 설정된 시간에 의하여 제어되며 배양이 끝나면 전체 전원이 자동으로 차단된다.(이상의 장치는 공지의 제어장치를 이용하므로 별도의 설명은 생략한다)The incubation time is controlled by the time set in the timer of the control device and when the incubation ends, the whole power is automatically cut off. (The above device uses a well-known control device, so a separate description is omitted).

이렇케하여 배양된 배양액은 배양조(1)의 배출관(10) 끝에 장치된 개폐밸브(B1)을 열어서 용기(60)에 받아 사용하게 되고 대형 혼합용기(복비,영양제)(61)에 배양액을 받을 경우 개폐밸브(B1)(B3)를 잠그고 개폐밸브(B4)의 출구에 호스를 연결하여 혼합용기(61)에 연결한후 양수기(41)를 작동시키면 혼합용기 내에 신속하게 배양액을 수용할 수 있다.The culture medium thus obtained is used to receive the container 60 by opening the shut-off valve (B1) provided at the end of the discharge pipe 10 of the culture tank (1) and receive the culture solution in a large mixed container (complex, nutrient) 61 In this case, by closing the shut-off valve (B1) (B3) and connecting the hose to the outlet of the shut-off valve (B4) to the mixing container 61, the water pump 41 is operated to quickly accommodate the culture medium in the mixing container .

한편 광합성 미생물을 배양할 때 원료 배양조(1)의 개방부(1c) 뚜껑(1d)을 열어넣고 투광기(6)를 점등하여 배양조내에 필요한 빛을 투입하게 되고 배양시간은 150시간이 적절하므로 컨트롤장치의 타이머를 이용하여 필요한 시간을 설정하여 배양이 가능하고 설정시간이 종료되면 다시 전원이 커지도록 함으로써 누구나 손쉽게 배양기를 운용할 수 있다.On the other hand, when culturing photosynthetic microorganisms, the lid 1d of the opening 1c of the raw material culture tank 1 is opened, and the light projector 6 is turned on to input the necessary light into the culture tank. Anyone can easily operate the incubator by setting the required time using the timer of the control device and cultivating it.

더우기 본 발명의 배양기는 배양조 내면 하측으로 장애물이 전혀 없고 교반기(5)가 배양조 상부에 장치되어 배양조로 공급되는 배양액의 공급압력에 따라 토출되는 토출압을 이용하여 교반하기 때문에 내부에 별도의 전원이 필요없게 되며 토출구가 연결관(50) 양측에 장치되어 있고 그 파생부에 개폐밸브(53)가 장치되어 있으므로 두개의 토출구로 배양액이 토출되게 하거나 일측 토출구로 배양액이 토출되게 선택할 수 있어서 소용돌이 속도를 조정할 수 있고 토출구가 토출하는 각도가 일정각으로 경사져 있으므로 소용될이가 배양조 상면에서 하단배출구에 이르도록 원추형의 소용돌이를 일으켜 내양액 깊숙이까지 산소공급이 가능하므로 안전한 배양이 이루어지고 배양액의 가열이 배양조 외부에서 이루어져 배양조로 공급이 되고 동시에 배양액가열기의 전기히터(22)가 망용기(21)에 장치되어 가열용기와 격리되어 있으므로 배양액이 순환될 때 뜨거운 전기히터와 직접적으로 접촉하는 것을 차단하여 간접적인 가열이 이루어지기 때문에 매우 안전한 가열이 가능하게 되는 이점이 있고 순환장치(4)에 양수기(41)가 장치되어 배양액의 순환과 배출에 도움을 주므로 사용이 편리하게 되는 이점을 얻을 수 있고 광합성미생물을 배양시킬 때는 조명장치(1g)에 장치된 투광기(6)를 통해 백열등빛이나 황색불빛을 조명하여 광합성 미생물의 배양을 효과적으로 할 수 있어서 실효성이 높은 배양기를 얻을 수 있 게 된다.Furthermore, the incubator of the present invention has no obstacles at the bottom of the inner surface of the incubator, and the agitator 5 is installed at the upper side of the incubator and is stirred using the discharge pressure discharged according to the supply pressure of the culture solution supplied to the incubator. No power is required, and the discharge port is provided on both sides of the connecting pipe 50 and the opening / closing valve 53 is provided at the derivative portion thereof, so that the culture medium can be discharged to two discharge ports or the culture solution can be discharged to one discharge port. The speed can be adjusted, and the angle discharged by the discharge port is inclined at a certain angle, so that the oxygen can be supplied deeply into the internal fluid by using a conical vortex so that the usefulness reaches from the upper side of the culture tank to the lower discharge port. The heating takes place outside the culture tank and is supplied to the culture bath. Since the electric heater 22 of the hot air is installed in the net container 21 and is separated from the heating container, very safe heating is possible because the indirect heating is performed by blocking direct contact with the hot electric heater when the culture medium is circulated. There is an advantage that the water pump 41 is provided in the circulation device (4) to help the circulation and discharge of the culture solution can be obtained the advantage that it is easy to use and when culturing photosynthetic microorganisms in the lighting device (1g) Through incandescent light 6, the incandescent light or the yellow light can be illuminated to effectively incubate photosynthetic microorganisms, thereby obtaining a highly effective incubator.

Claims (4)

스텐레스스틸로 제작되어 지지각(1a)에 의하여 일정위치에 장치되고 균주투입구(1b)와 뚜껑(1d)가 있는 개방부(1c)를 가진 원통형의 배양조(1)와 Cylindrical culture tank (1) made of stainless steel and installed at a predetermined position by the support angle (1a) and having an opening (1c) having a strain inlet (1b) and a lid (1d); 배양조 외부에 가열원통(20)이 장치되어 그 내부에 다공망통(21)이 내장되고 다공망통속에 전기히터(22)가 장치되어 배양액을 가열하여 배양조내에 공급하는 배양액가열기(2)와 A heating cylinder 20 is installed outside the culture tank, and a porous network 21 is embedded therein, and an electric heater 22 is installed in the porous network cylinder to heat the culture solution and supply the culture medium to the culture tank. 배양조 하측에 장치되어 배양액을 흡인하여 이를 배양액 가열기로 공급하는 양수기(41)가 달린 순환장치(4)와 A circulation device (4) having a water pump (41) installed at the lower side of the culture tank to suck the culture solution and supply it to the culture medium heater; 배양액가열기(2)에서 배양조로 공급되는 공급관(23)에 연결되고 연결관(50) 양측에 토출구(51)(52)가 설치되어 그 파생부에 개폐밸브(53)가 장치된 교반기(5)와 Agitator (5) connected to the supply pipe (23) supplied from the culture medium heater (2) to the culture tank, and discharge ports (51) (52) are provided at both sides of the connecting pipe (50), and the opening / closing valve (53) is provided at its derivative part. Wow 배양조의 주벽 일측에 투광판(1e)이 장치되어 그 속에 조명실(1f)을 만들고 조명실 내부에서 배양조로 조명되게 투광기(6)와The floodlight plate 1e is installed at one side of the circumferential wall of the culture tank to make the illumination chamber 1f therein, and the light projector 6 and the illumination chamber are illuminated by the culture tank inside the lighting chamber. 자외선멸균등(8)을 장치한 조명장치(1g)와 온도감지센서(3)가 구비되어 전기콘트롤장치(7)로 제어되고 운용되는 구성의 토양미생물 배양기Soil microorganism incubator with a lighting device (1g) equipped with ultraviolet sterilization lamp (8) and a temperature sensor (3) is provided and controlled and operated by the electric control device (7) 상기1항에서 In 1 above 배양액가열기는 가열원통(20) 내부에 다공망통(21)이 장치되어 그 속에 전기 히터(22)가 장치되고 다공망통(21)의 노출부(21a)에 보충수공급통(24)이 장치되며 가열원통(20) 상단측내측에서 배양조(1)속으로 공급관(23)이 형성되어 가열된 배양액을 배양조(1)내에 공급하도록 한 것을 포함하는 토양미생물 배양기The culture medium heater is equipped with a porous network 21 inside the heating cylinder 20, the electric heater 22 is installed therein and the supplementary water supply cylinder 24 is provided in the exposed portion 21a of the porous network 21. Soil microorganism incubator comprising a feed tube 23 is formed into the culture tank (1) in the upper side of the heating cylinder 20 to supply the heated culture solution into the culture tank (1) 상기1항에서In 1 above 순환장치(4)는 배양조 하측 지지각 사이에 장치판(40)을 부착하고 그 위에 양수기(41)를 장치하여 그 흡입관(41a)을 배양조(1) 하단에서 외부로 형성되는 배출관(10)의 중간연결부(10a)와 연결관(42)으로 연결하고 연결부에 개폐밸브(B2)를 장치하며 배출관(10) 끝에 개폐밸브(B1)를 장치하고 양수기(41)의 토출관(41b)은 가열원통(20)의 하단 유입구(20a)와 연결관(43)으로 연결하여 가열원통 입구에 개폐밸브(B3)를 장치하고 그 하측에서 외부로 형성되게 배출관(44)을 부착하여 배출관(44) 출구에 개폐밸브(B4)를 장치하여 배양액을 순환시키거나 인출할 수 있게 한 것을 포함하는 토양미생물 배양기The circulation device 4 attaches the device plate 40 between the lower support angles of the culture tank, and installs a water pump 41 thereon, so that the suction pipe 41a is formed from the lower side of the culture tank 1 to the outside. Is connected to the middle connecting portion (10a) and the connecting pipe (42), and the opening and closing valve (B2) in the connecting portion is provided with the opening and closing valve (B1) at the end of the discharge pipe (10) and the discharge pipe (41b) of the water pump 41 is Connected to the lower inlet 20a of the heating cylinder 20 and the connection pipe 43, an opening / closing valve B3 is installed at the inlet of the heating cylinder, and a discharge pipe 44 is attached to the outside of the heating cylinder 20 so as to be formed outside. Soil microorganism incubator comprising the opening and closing valve (B4) at the outlet to circulate or withdraw the culture medium 상기1항에서 In 1 above 교반기(5)는 배양액가열기(2)의 공급관(23)에 연결되도록 연결관(50)을 설치하여 그 입구와 출구측에 일정각도로된 토출구(51)(52)를 장치하고 입구측 토출구(51)와 출구측 토출구(52)가 연결되는 중간 파생부에 개폐밸브(53)를 설치하여서 토출되는 압력으로 배양액을 교반시킬 수 있게 한 것을 포함하는 토양미생물 배양기The stirrer 5 is provided with a connecting pipe 50 so as to be connected to the supply pipe 23 of the culture medium heater 2, and equipped with discharge ports 51 and 52 at a predetermined angle on the inlet and outlet sides thereof, and the inlet discharge port ( 51) and the soil microorganism incubator comprising the opening and closing valve 53 is installed in the intermediate derivation portion to which the outlet side outlet 52 is connected to allow the culture solution to be stirred at the discharged pressure.
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KR100967144B1 (en) * 2009-07-14 2010-07-05 김의수 Liquid fertilizer producing apparatus
KR101052625B1 (en) * 2009-05-08 2011-07-29 정우종 Indirect Heated Microbial Incubator
KR102002469B1 (en) 2018-06-21 2019-07-23 주식회사 이시스 Apparatus for cultivating microbe using non-heating means
CN113999759A (en) * 2021-11-02 2022-02-01 中国农业科学院农田灌溉研究所 Soil microorganism culture device and method
KR102608770B1 (en) * 2023-04-27 2023-12-01 홍종일 Ultraviolet Sterilization Device for Effective Microorganism Culture Medium Charge Pipe

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KR100353893B1 (en) 2002-03-08 2002-09-28 Korea Atomic Energy Res Automatic control system of pilot-scale bioreactor
KR20040067254A (en) * 2003-01-22 2004-07-30 에스케이케미칼주식회사 Bioreactor for Cultivating Bacteria in Wastewater treatment System
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101052625B1 (en) * 2009-05-08 2011-07-29 정우종 Indirect Heated Microbial Incubator
KR100967144B1 (en) * 2009-07-14 2010-07-05 김의수 Liquid fertilizer producing apparatus
KR102002469B1 (en) 2018-06-21 2019-07-23 주식회사 이시스 Apparatus for cultivating microbe using non-heating means
CN113999759A (en) * 2021-11-02 2022-02-01 中国农业科学院农田灌溉研究所 Soil microorganism culture device and method
CN113999759B (en) * 2021-11-02 2023-08-04 江苏农林职业技术学院 Soil microorganism culture device and method
KR102608770B1 (en) * 2023-04-27 2023-12-01 홍종일 Ultraviolet Sterilization Device for Effective Microorganism Culture Medium Charge Pipe

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