KR20060120867A - Microorganism medium - Google Patents

Microorganism medium Download PDF

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Publication number
KR20060120867A
KR20060120867A KR1020050043093A KR20050043093A KR20060120867A KR 20060120867 A KR20060120867 A KR 20060120867A KR 1020050043093 A KR1020050043093 A KR 1020050043093A KR 20050043093 A KR20050043093 A KR 20050043093A KR 20060120867 A KR20060120867 A KR 20060120867A
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KR
South Korea
Prior art keywords
light source
cover
incubator
culture medium
hole
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KR1020050043093A
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Korean (ko)
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유화성
박우상
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박우상
유화성
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Priority to KR1020050043093A priority Critical patent/KR20060120867A/en
Publication of KR20060120867A publication Critical patent/KR20060120867A/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/36Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C12M1/38Temperature-responsive control
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/20Material Coatings
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/04Apparatus for enzymology or microbiology with gas introduction means
    • C12M1/06Apparatus for enzymology or microbiology with gas introduction means with agitator, e.g. impeller
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/04Apparatus for enzymology or microbiology with gas introduction means
    • C12M1/08Apparatus for enzymology or microbiology with gas introduction means with draft tube
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/10Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

Abstract

A microorganism incubator is provided to reduce a risk of breakage by using stainless steel, and inhibit temperature change of culture medium by installing an electric heater, and blocking temperature increase by a light source, so that cultivation effects of microorganisms are promoted. The microorganism incubator(1) made of stainless steel comprises: a transparent window(1a) in the main wall; a releasing pipe(1b) under the incubator; the electric heater(2) on the outside surface of bottom board; a gauge board(3) in front of the incubator cover(1c); a geared motor(4) in the center of the cover; a stirrer(5) connected to a rotation axis(4a) of the geared motor(4), and having two stirring wings(5, 5a); a light source-protecting vessel(8) which contains a sterilizing lamp(6) and a halogen lamp(7), and is located in a hole(101) in one side of the cover(1c) and projected in a downward direction; a ventilation pan(9) in the light source-protecting vessel through a ventilation hole(8a); an inlet(102) located in the opposite side of the light source-protecting vessel on the incubator cover; a cover of the inlet(103); and a temperature sensor(S) in the culture medium.

Description

미생물 배양기{MICROORGANISM MEDIUM}Microbial incubator {MICROORGANISM MEDIUM}
도 1은 본 발명의 단면도1 is a cross-sectional view of the present invention
도 2는 본 발명의 평면도2 is a plan view of the present invention
도 3은 본 발명의 교반기 사시도이다.3 is a perspective view of the agitator of the present invention.
* 도면의 주요부분에 대한 부호설명* Explanation of symbols on the main parts of the drawings
1 : 배양조 1a : 투시창1: culture tank 1a: viewing window
1b : 배출관 1c : 배양조뚜껑1b: discharge pipe 1c: culture tank lid
2 : 전기히터 4 : 모터2: electric heater 4: motor
4a : 회전축 5,5a : 판상교반익4a: rotating shaft 5,5a: plate stirring blade
6 : 멸균등 6a,7a : 등보호통6: sterilization lamp 6a, 7a: back protection tube
8 : 광원장치통 9 : 배기팬8: light source device 9: exhaust fan
50 : 교반기 101 : 구멍50: stirrer 101: hole
102 : 투입구 103 : 덮개102: inlet 103: cover
S : 센서S: Sensor
본 발명은 스테인레스스틸제로 배양조를 만들어 외면에 전기히터를 부착하고 상부에 배기팬이 부설된 광원장치 통을 부착하며 배양조 중심부에 판상으로된 두개의 수직교반기를 나란히 투입하여 회전시킴으로써 배양액에서 발생한 거품의 범람을 막는 교반이 가능하고 광원에서 발생하는 열기가 배양액의 온도 상승에 관여하지 않게하여 배양액의 일정온도를 유지할 수 있게 하므로서 미생물의 배양 효과를 촉진할 수 있는 미생물 배양기기가 되게 한 것이다.In the present invention, the culture tank is made of stainless steel, and an electric heater is attached to the outer surface, and a light source device tube equipped with an exhaust fan is attached to the outer surface, and two vertical stirrers are placed side by side in the center of the culture tank to rotate. It is possible to stir to prevent the overflow of bubbles and to maintain the constant temperature of the culture medium so that the heat generated from the light source does not participate in the temperature rise of the culture medium to be a microbial culture device that can promote the culture effect of the microorganisms.
종래의 미생물 배양기는 배양조가 유리로 되어 있어서 파손위험이 있기 때문에 조심을 해야하고 전기히터를 내부에 장치하기 때문에 종균에 영향을 줄 뿐 아니라 광원이 배양조 내에 노출되어 있어서 광원에서 발생하는 열기가 배양액에 작용하여 온도상승을 초래하는 원인이 되므로 냉각장치를 하는 등으로 광원관리에 조심을 해야 하는 등의 문제가 있었다.Conventional microbial incubators have to be careful because the culture tank is made of glass, which is a risk of damage, and because the electric heater is installed inside, it not only affects the spawn, but the heat generated from the light source is exposed to the culture medium. There is a problem such as need to be careful in the light source management, such as the cooling device because it causes a rise in temperature by acting on.
본 발명은 상기와 같은 종래의 불편을 시정하기 위하여 개발한 것으로 이를 첨부된 도면에 의거 상세히 설명하면 다음과 같다.The present invention was developed in order to correct the conventional inconvenience as described above in detail with reference to the accompanying drawings as follows.
스테인레스스틸제 통으로 된 배양조(1)의 주벽에 투시창(a)을 형성하고 하단에 배출관(1b)이 형성하여 밑판 외면에 전기히터(2)를 밀착고정시키고 배양조 뚜껑(1c)에는 전면에 계기판(3)을 부착하여 전기 회로를 내장하고 중심부에 기어드모터(4)를 장치하여 그 회전축(4a)을 교반기 내부로 투입하여 회전축 하단에 일정각도를 가지고 세폭으로 되는 두개의 판상교반익(5)(5a)을 나란하게 수직으로 배치한 교반기(50)를 부착하여 교반익(5)(5a)의 하단이 배양액 속으로 투입되게 하고 배양조 뚜껑(1c) 일측에 구멍(101)을 뚫어 멸균등(6)과 할로겐등(7)이 장치되는 투명체의 등보호통(6a)(7a)이 하단으로 돌출되게 형성된 4각통체로 광원장치통(8)을 장착하여 등보호통(6a)(7a)이 배양조내부로 투입되게 하고 광원장치통 중앙상부에 배기공(8a)을 뚫어 그 내측에 배기팬(9)을 장치하며 광원장치통이 장치된 반대측에 투입구(102)를 형성하여 덮개(103)를 착탈할 수 있게 하고 내부에 온도센서(S)를 장치하여서 되는 구성이다.A viewing window (a) is formed on the circumferential wall of the culture tank (1) made of stainless steel, and the discharge pipe (1b) is formed at the bottom to fix the electric heater (2) in close contact with the bottom plate, and the front of the culture tank lid (1c). Attach the instrument panel (3) to embed the electric circuit and equip the geared motor (4) in the center, and put the rotary shaft (4a) into the stirrer, two plate stirwing blades of narrow width with a certain angle at the bottom of the rotary shaft (5) Attach the stirrer 50 arranged vertically side by side (5a) so that the lower end of the stirring blade (5) (5a) is introduced into the culture medium, and the hole 101 is sterilized by drilling a hole 101 on one side of the culture tank lid (1c). The light source device cylinder 8 is a quadrangular body formed so that the back protection cylinders 6a and 7a of the transparent body on which the lamp 6 and the halogen lamp 7 are mounted protrude downward. Into the inside of the culture tank, the exhaust hole (8a) is drilled in the upper part of the center of the light source device and the exhaust fan (9) In this case, the inlet 102 is formed on the opposite side where the light source unit is installed, and the cover 103 can be attached and detached, and the temperature sensor S is installed therein.
이와같이된 본 발명은 배양조(1)가 스테인레스스틸제로 되어 주벽에 투명체로 된 투시창(1a)이 있어서 내부에 채워놓은 배양액을 외부에서 관찰할 수 있고 스테인레스스틸제 통이므로 내구적이고 위생적이며 파손의 위험이 없고 밑판에 전기히터(2)를 부착하여 밀착시킴으로써 전열을 배양조에 정확히 전달하면서 종균에 직접적인 피해를 주지 않아 안전한 이점이 있다.In the present invention as described above, the culture tank 1 is made of stainless steel, and the periphery is a transparent window on the circumferential wall 1a so that the culture solution filled therein can be observed from the outside and is made of stainless steel, which is durable, hygienic and risk of damage. There is no safety by having no direct damage to the spawn while correctly transferring heat to the culture tank by attaching the electric heater 2 to the bottom plate and bringing it into close contact.
또한 본 발명은 광원장치통(8)이 독립된 통체로되어 밑에 투명체로 된 등보호통(6a)(7a)이 돌출되어 그 내부에 각각 할로겐등(7)과 멸균등(6)이 장치되고 중간부에 배기통(8a)이 있어서 등기가 배양약과 분리되고 또 배기팬(9)을 장치하기 때문에 광원에서 발생하는 열기를 외부로 배출하여 광원에서 발생하는 열기가 교반액의 온도상승에 영향을 주지 않게되고 필요한 광원만을 공급해 주는 효과를 낼 수 있으며 교반기(50)는 일정각도를 가지고 세폭으로된 두개의 판상교반익(5)(5a)이 나란히 수직으로 설치되어 교반액속에 투입되어 회전하므로 배양액을 교반할때 소용돌이를 일으키어 배양액에서 발생한 거품이 외부로 넘치는 것을 방지하므로 안전한 교반이 이루어지고 산소공급을 충분히 할 수 있는 이점을 낼 수 있고 온도센서와 전기히터를 통상의 전기회로 연결하여 미생물 배양에 필요한 일정온도를 유지할 수 있으므로 종래보다 신속하게 필요한 미생물을 배양시킬 수 있는 이점을 얻을 수 있게 되는 것이다.In addition, in the present invention, the light source device cylinder 8 is an independent cylinder, and the lower protective cylinders 6a, 7a, which are transparent bodies, are protruded, and a halogen lamp 7 and a sterilization lamp 6 are respectively installed therein. In the exhaust tube 8a, the lamp is separated from the culture medicine and the exhaust fan 9 is installed, so that the heat generated from the light source is discharged to the outside so that the heat generated from the light source does not affect the temperature rise of the agitating liquid. It can have the effect of supplying only the necessary light source, and the agitator 50 has a predetermined angle and two plate stirring blades (5) (5a) having a narrow width are installed side by side in a vertical manner and are added to the stirring solution to rotate the culture solution. When the vortex is generated to prevent the bubbles generated from the culture from overflowing to the outside, it is possible to achieve safe agitation and supply sufficient oxygen. By connecting the circuit it is possible to maintain a constant temperature necessary for culturing microorganisms will be able to obtain the advantage of culturing the required microorganisms faster than before.

Claims (1)

  1. 스테인레스스틸제 통으로 된 배양조(1)의 주벽에 투시창(a)을 형성하고 하단에 배출관(1b)을 형성하여 밑판 외면에 전기히터(2)를 밀착고정시키고 배양조 뚜껑(1c)에는 전면에 계기판(3)을 부착하여 전기 회로를 내장하고 중심부에 기어드모터(4)를 장치하여 그 회전축(4a)을 교반기 내부로 투입하여 회전축 하단에 일정각도를 가지고 세폭으로 되는 두개의 판상교반익(5)(5a)을 나란하게 수직으로 배치한 교반기(50)를 부착하여 교반익(5)(5a)의 하단이 배양액 속으로 투입되게 하고 배양조 뚜껑(1c) 일측에 구멍(101)을 뚫어 멸균등(6)과 할로겐등(7)이 장치되는 투명체의 등보호통(6a)(7a)이 하단으로 돌출되게 형성된 4각통체로된 광원장치통(8)을 장착하여 등보호통(6a)(7a)이 배양조내부로 투입되게 하고 광원장치통 중앙상부에 배기공(8a)을 뚫어 그 내측에 배기팬(9)을 장치하여 광원장치통이 장치된 반대측에 투입구(102)를 형성하여 덮개(103)를 착탈할 수 있게 하고 내부에 배양액 속에 온도센서(S)를 장치하여서 되는 미생물 배양기A viewing window (a) is formed on the circumferential wall of the culture tank (1) made of stainless steel tub, and the discharge pipe (1b) is formed at the bottom, and the electric heater (2) is fixed to the outer surface of the bottom plate, and the front of the culture tank lid (1c) Attach the instrument panel (3) to embed the electric circuit and equip the geared motor (4) in the center, and put the rotary shaft (4a) into the stirrer, two plate stirwing blades of narrow width with a certain angle at the bottom of the rotary shaft (5) Attach the stirrer 50 arranged vertically side by side (5a) so that the lower end of the stirring blade (5) (5a) is introduced into the culture medium, and the hole 101 is sterilized by drilling a hole 101 on one side of the culture tank lid (1c). The back protection cylinder 6a (7a) is equipped with a light source device cylinder 8 made of a quadrangular body formed such that the back protection cylinder 6a, 7a of the transparent body on which the lamp 6 and the halogen lamp 7 are mounted protrudes downward. Into the inside of the culture tank, and through the exhaust hole (8a) in the upper part of the center of the light source device, the exhaust fan (9) Device to the light source tube is able to removably the cover 103 to form a charging port 102 on the device side, are microorganisms hayeoseo device with a temperature sensor (S) in the culture medium inside the culture medium
KR1020050043093A 2005-05-23 2005-05-23 Microorganism medium KR20060120867A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100880825B1 (en) * 2008-10-09 2009-01-30 송정화 Incubator for microorganism medium
KR100930596B1 (en) * 2009-05-07 2009-12-09 주식회사 쉴드텍 Integrated cultivating device for proliferation and supply of useful microorganism
KR101155137B1 (en) * 2009-12-07 2012-06-11 (주)비앤피테크 Micro plate for bioreactor
KR101155136B1 (en) * 2009-06-26 2012-06-12 (주)비앤피테크 Multi-channel photobioreactor for culturing photosynthetic microorganisms
KR101235825B1 (en) * 2011-05-31 2013-02-21 경상북도(농업기술원) Microorganism medium
KR101641373B1 (en) * 2016-01-22 2016-07-29 한국미강연합유통 주식회사 Multiple sterilization for rice bran
US20180105977A1 (en) * 2015-04-09 2018-04-19 Colorifix Limited Method of dyeing fabric using microorganisms

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100880825B1 (en) * 2008-10-09 2009-01-30 송정화 Incubator for microorganism medium
KR100930596B1 (en) * 2009-05-07 2009-12-09 주식회사 쉴드텍 Integrated cultivating device for proliferation and supply of useful microorganism
KR101155136B1 (en) * 2009-06-26 2012-06-12 (주)비앤피테크 Multi-channel photobioreactor for culturing photosynthetic microorganisms
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