KR100604095B1 - Microorganism medium - Google Patents

Microorganism medium Download PDF

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KR100604095B1
KR100604095B1 KR1020040040438A KR20040040438A KR100604095B1 KR 100604095 B1 KR100604095 B1 KR 100604095B1 KR 1020040040438 A KR1020040040438 A KR 1020040040438A KR 20040040438 A KR20040040438 A KR 20040040438A KR 100604095 B1 KR100604095 B1 KR 100604095B1
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culture tank
culture
outer cylinder
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KR20050115123A (en
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유화성
천종구
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유화성
천종구
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    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
    • C12M1/06—Apparatus for enzymology or microbiology with gas introduction means with agitator, e.g. impeller
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20—Degassing; Venting; Bubble traps
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

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Abstract

본 발명은 각종 미생물을 편리하게 배양시킬 수 있는 미생물 배양기에 관한 것인데 보다 구체적인 것은 배양조 외면에 가열실과 광원부를 구비하여 가열등과 형광등과 배기팬을 설치하고 배양조 내부에 온도감지센서와 공기공급기와 교반기를 설치하면서 거품제거용 돌기를 배치하여 이들을 전동운전장치와 결합시켜서 운용할 수 있게 함으로써 각종 미생물을 편리하게 배양시킬 수 있게 한 것으로 구체적인 것은The present invention relates to a microbial incubator capable of conveniently cultivating various microorganisms, and more specifically, a heating chamber and a light source unit are installed on the outer surface of the culture tank to install a heating lamp, a fluorescent lamp and an exhaust fan, and a temperature sensor and an air supply inside the culture tank. And by installing the agitator and the bubble removing projections by placing them in combination with the electric driving device to be able to operate a variety of microorganisms convenient

유리 또는 투명 합성수지제로 성형된 원통형 용기로 되고 상단에 물주입구(105)와 원료투입구(1b)가 있는 덮개(1a)가 장치되며 하단에 배출구(1c)가 구비되어 외통(2)내에 장치되는 배양조(1)와 Cultivation is formed in a cylindrical container formed of glass or transparent synthetic resin, the cover (1a) having a water inlet 105 and the raw material inlet (1b) at the top and an outlet (1c) at the bottom is provided in the outer cylinder (2) Joe (1) and

배양조 외면에 일정간격을 두고 가열실(200)이 형성되게 상부에 덮개(2a)가 덮혀지고 바닥이 막혀지며 바닥일측에서 배기팬(204)이 장치된 외통체(2)와 Covered with a cover (2a) and the bottom is clogged at a predetermined interval on the outer surface of the culture tank to form a heating chamber 200 and the outer cylinder (2) equipped with an exhaust fan 204 on the bottom side;

배양조(1) 바닥 내측으로 장치되어 배양조내에 일정량의 공기를 주입하는 공기공급기(100)와 The air supply unit 100 is installed inside the bottom of the culture tank 1 to inject a predetermined amount of air into the culture tank.

배양조내부에 설치되어 배양조의 온도를 감지하는 온도센서(101)와 Installed in the culture tank and the temperature sensor 101 for sensing the temperature of the culture tank and

배양조 주벽 상단부에서 내측으로 돌출되는 거품제거용 돌기(102)와 Defoaming projection 102 and protrudes inward from the upper end of the culture vessel main wall

배양조 덮개에 장치된 전동기(103a)의 구동축에 연결되어 배양조 위치하는 전동교반기(103)와 An electric stirrer 103 connected to the drive shaft of the electric motor 103a installed in the culture tank cover and positioned in the culture tank;

배양조 덮개에 장치되어 배양조내부의 압력을 표시하는 압력계(104)와 A pressure gauge 104 mounted on the cover of the culture tank to display the pressure inside the culture tank;

외통체(2) 주벽에는 가열실 내부로 향해 장치되어 배양조에 열기를 공급하는 백열전등(201)과 외통체 주벽에서 가열실내부로 향해 장치되어 배양조에 빛을 공급하는 형광등(202)과 외통체 주벽에서 가열실 내부로 향해 장치되어, 배양조로 조명되는 자외선 살균등(203)과 상기 기구들을 적기에 작동시키고 끌 수 있게 구성된 스위치와 장시간 타이머가 구비된 전기회로로된 제어기(3)로 이루어지는 미생물 배양기이다.The outer wall of the outer cylinder 2 has an incandescent lamp 201 that is installed toward the inside of the heating chamber to supply heat to the culture chamber and a fluorescent lamp 202 that is installed from the outer wall of the outer cylinder to the inside of the heating chamber to supply light to the culture chamber and the outer cylinder main wall. Microorganism incubator consisting of an ultraviolet circuit sterilizer 203, which is directed toward the interior of the heating chamber, which is illuminated by a culture tank, an electric circuit controller 3 equipped with a switch configured to timely operate and turn off the apparatus and a long time timer. to be.

미생물, 배양기, 배양조, 외통체Microorganism, Incubator, Culture Tank, External Body

Description

미생물 배양기{MICROORGANISM MEDIUM} Microbial incubator {MICROORGANISM MEDIUM}             

도 1은 본 발명의 외관 사시도로서 덮개를 분리한 예시도1 is an exploded perspective view of the present invention showing the cover removed

도 2는 본 발명의 단면도2 is a cross-sectional view of the present invention

도 3은 본 발명의 또 다른 실시상태를 보이는 분해 사시도Figure 3 is an exploded perspective view showing another embodiment of the present invention

도 4는 도3의 단면도4 is a cross-sectional view of FIG.

도 5는 본 발명의 횡단 평면도5 is a cross-sectional plan view of the present invention.

도 6은 본 발명의 작동 회로예시도이다.6 is an exemplary operation circuit diagram of the present invention.

* 도면의 주요부분에 대한 부호설명* Explanation of symbols on the main parts of the drawings

1 : 배양조 1b : 원료투입구1: culture tank 1b: raw material inlet

1c : 배출구 2 : 외통체1c: outlet 2: outer cylinder

3 : 제어기 100 : 공기공급기3: controller 100: air supply

101 : 온도센서 102 : 거품제거용돌기101: temperature sensor 102: bubble removing projection

103 : 전동교반기 104 : 압력계103: electric stirrer 104: pressure gauge

105 : 물주입구 201 : 백열전등105: water inlet 201: incandescent lamp

202 : 형광등 203 : 살균등202: fluorescent lamp 203: germicidal lamp

본 발명은 각종 미생물을 편리하게 배양시킬 수 있는 배양기에 관한 것인데 보다 구체적인 것은 배양조 외면에 가열실과 광원부를 구비하여 가열등과 형광등과 배기팬을 설치하고 배양조 내부에 온도감지센서와 공기공급기와 교반기를 설치하면서 거품제거용 돌기를 배치하여 이들을 전동운전장치와 결합시켜서 관리할 수 있게 제어기를 구비함으로써 각종 미생물을 편리하게 배양시킬 수 있게 한 것이다.The present invention relates to an incubator capable of cultivating various microorganisms conveniently. More specifically, a heating chamber and a light source unit are provided on the outer surface of the culture tank to install a heating lamp, a fluorescent lamp, and an exhaust fan, and a temperature sensor and an air supply unit in the culture tank. By installing a stirrer and placing a bubble removing projections by combining them with an electric drive device to provide a controller that can be managed to conveniently culture various microorganisms.

최근에는 다양한 미생물을 농업이나 축산에 많이 이용하고 있고 농업이나 축산의 경우 선택한 미생물을 배양하여 물에 희석시킨 다음 토양에 뿌리거나 가축에게 먹이는 방법으로 작물의 생산을 촉진시키고 병충해를 방지하며 가축의 경우 성장을 촉진시키고 질병을 예방하게 되고 육질을 좋게 하며 배설물의 약취와 오염을 줄일 수 있게 되는 효과를 얻고 있다.Recently, various microorganisms are used in agriculture or livestock raising, and in agriculture or livestock breeding, the selected microorganisms are cultivated and diluted in water, then sprinkled on soil or fed to livestock to promote crop production and prevent pests. It has been shown to promote growth, prevent disease, improve meat quality, and reduce fecal intoxication and pollution.

따라서 이러한 미생물을 일정한 영양분과 온도와 빛을 이용하여 배양시키는 것이므로 장시간에 걸친 관리를 요하게 되고 이를 위해 카비넷 형상으로된 배양기가 주로 사용되고 있다.Therefore, since the microorganisms are cultured using a constant nutrient, temperature and light, it requires management for a long time and a cabinet-shaped incubator is mainly used for this.

그러나 일손이 바쁜 농촌에서는 이러한 조건을 만족시키는 것이 불가능하므로 만족스러운 효과를 얻지 못하는 실정에 있다.However, it is not possible to satisfy these conditions in a busy rural village, so it is impossible to obtain a satisfactory effect.

본 발명은 상기와 같은 종래의 결점을 시정하여 농촌에서 보다 편리하게 사용할 수 있고 배양효과를 높일 수 있는 안정된 배양기를 제공하는 것이다.The present invention is to provide a stable incubator that can be used more conveniently in rural areas and to improve the culture effect by correcting the above conventional drawbacks.

이를 보다 상세히 첨부된 도면에 의거 상세히 설명하면 다음과 같다.
When described in detail based on the accompanying drawings in more detail as follows.

본 발명은 유리 또는 투명 합성수지제로 성형된 원통형 용기로 되고 상단에 물주입구(105)와 원료투입구(1b)가 있는 덮개(1a)가 장치되며 하단에 배출구(1c)가 구비되어 외통(2)내에 장치되는 배양조(1)와
배양조 외면에 일정간격을 두고 가열실(200)이 형성되게 상부에 덮개(2a)가 덮혀지고 바닥이 막혀지며 바닥일측에서 배기팬(204)이 장치된 외통체(2)와
배양조(1) 바닥 내측으로 장치되어 배양조내에 일정량의 공기를 주입하는 공기공급기(100)와
배양조내부에 설치되어 배양조의 온도를 감지하는 온도센서(101)와
배양조 주벽 상단부에서 내측으로 돌출되는 거품제거용 돌기(102)와
배양조 덮개에 장치된 전동기(103a)의 구동축에 연결되어 배양조 위치하는 전동교반기(103)와
배양조 덮개에 장치되어 배양조내부의 압력을 표시하는 압력계(104)와
외통체(2) 주벽에는 가열실 내부로 향해 장치되어 배양조에 열기를 공급하는 백열전등(201)과 외통체 주벽에서 가열실내부로 향해 장치되어 배양조에 빛을 공급하는 형광등(202)과 외통체 주벽에서 가열실 내부로 향해 장치되어, 배양조로 조명되는 자외선 살균등(203)과 상기 기구들을 적기에 작동시키고 끌 수 있게 구성된 스위치와 장시간 타이머가 구비된 전기회로로된 제어기(3)로 이루어지는 미생물 배양기이다.The present invention is a cylindrical container formed of glass or transparent synthetic resin and is provided with a cover 1a having a water inlet 105 and a raw material inlet 1b at the top, and an outlet 1c at the bottom, which is provided in the outer cylinder 2. The culture tank (1)
Covered with a cover (2a) and the bottom is clogged at a predetermined interval on the outer surface of the culture tank to form a heating chamber 200 and the outer cylinder (2) equipped with an exhaust fan 204 on the bottom side;
The air supply unit 100 is installed inside the bottom of the culture tank 1 to inject a predetermined amount of air into the culture tank.
Installed in the culture tank and the temperature sensor 101 for sensing the temperature of the culture tank and
Defoaming projection 102 and protrudes inward from the upper end of the culture vessel main wall
An electric stirrer 103 connected to the drive shaft of the electric motor 103a installed in the culture tank cover and positioned in the culture tank;
A pressure gauge 104 mounted on the cover of the culture tank to display the pressure inside the culture tank;
The outer wall of the outer cylinder 2 has an incandescent lamp 201 that is installed toward the inside of the heating chamber to supply heat to the culture chamber and a fluorescent lamp 202 that is installed from the outer wall of the outer cylinder to the inside of the heating chamber to supply light to the culture chamber and the outer cylinder main wall. Microorganism incubator consisting of an ultraviolet circuit sterilizer 203, which is directed toward the interior of the heating chamber, which is illuminated by a culture tank, an electric circuit controller 3 equipped with a switch configured to timely operate and turn off the apparatus and a long time timer. to be.

이와같이된 본 발명은 배양조(1)내에 배양액을 넣고 선택된 미생물을 주입하 여 배양을 시키게 되는데 (배양액은 질소, 인산, 카리, 규산, 칼슘, 마그네슘, 망간, 붕소, 철, 몰리브덴, 아연, 구리, 한천, 혼합물로 조성된 유기물 배지 5kg에 물200ℓ를 주입하는 비율로 배양액을 만들어 미생물 200g를 넣는 비율로 조성함) 스위치SW-1을 ON하면 전원이 공급되면서 10시간에서 300 시간용 타이머1 이 동작하여 릴레이 1접점을 통해 타이머2에 전원이 공급되고 릴레이 2에 의하여 자외선 살균등(203)이 점등되어 배양액을 살균하게 되며 타이머 2에 설정된 시간이 경과되면 릴레이 2가 복귀되어 살균등은 꺼지고 복귀접점에 의하여 삼파장, 형광등, 교반기, 기포발생기, 온도감지기 및 부저에 전원이 공급되어 부저가 눌리면 준비된 미생물 균주를 투입하여 배양을 시작하고 온도감지기의 설정온도에 따라 릴레이3의 동작접점에 의하여 배양액 가열용 백열전등과 냉각팬이 교대로 동작하여 배양액의 온도를 약30도로 유지하여 주며 타이머1의 설정시간이 경과되면 전체전원이 꺼지게 되고 배양이 완성되는 것이다.In the present invention, the culture solution is placed in the culture tank 1 and the selected microorganisms are injected to incubate the culture. , Make a culture solution at the rate of injecting 200 liters of water into 5 kg of organic medium composed of agar, agar, and mixture, and make it at the rate of adding 200 g of microorganisms.) When the switch SW-1 is turned on, timer 1 for 10 to 300 hours is supplied while power is supplied. The timer 2 is supplied to the timer 2 through the relay 1 contact, and the ultraviolet sterilization lamp 203 is turned on by the relay 2 to sterilize the culture solution.When the time set in the timer 2 has elapsed, the relay 2 is restored and the sterilization lamp is turned off and returned. Power is supplied to three wavelengths, fluorescent lamps, stirrers, bubble generators, temperature detectors and buzzers by contact point. According to the set temperature of the high temperature sensor, the incandescent lamp for heating the medium and the cooling fan are operated alternately by the operating contact of the relay 3 to maintain the temperature of the medium about 30 degrees. And the culture is completed.

따라서 본 발명은 호기성 균주를 비롯하여 광합성미생물 균주까지 배양을 시킬 수 있도록 되고 10시간에서 300시간 타이머를 채용하여 장시간의 배양온도관리를 달성하게 되며 외통에 장치되는 백열전등은 200W 용량의 백열등 2개를 장치하면 7시간 만에 배양액을 30℃까지 가온할 수 있어서 백열전등으로 배양액을 충분히 가열할 수 있고 백열전등과 형광등 및 자외선 살균등은 교체가 용이하므로 고장시 신속한 수리를 통해 지속적인 배양을 달성하게 된다.Therefore, the present invention is capable of culturing even aerobic strains and photosynthetic microorganism strains, and employing a timer for 10 hours to 300 hours to achieve long-term culture temperature management, and the incandescent lamps installed in the external cylinder are two 200W incandescent lamps. With this device, the culture medium can be heated to 30 ℃ in 7 hours, so that the culture medium can be sufficiently heated by incandescent lamps, and incandescent lamps, fluorescent lamps and UV sterilization lamps are easy to replace, so that continuous culture can be achieved through rapid repair in case of failure. .

또 본 발명의 배양조에는 상단부 주벽내측에 거품제거용 돌기(102)가 설치되어 있으므로 배양중에 발생하는 거품을 제거하여 거품이 압력계의 입구에 부착하거나 외부로 넘치는 등으로 배양저해요소를 해소시킬 수 있는 이점이 있어서 농촌에서 사용하기에 편리한 배양기를 얻는 것이다.In addition, the culture tank of the present invention is provided with a bubble removing projection 102 in the inner peripheral wall of the upper end to remove the bubbles generated during the culture to eliminate the culture inhibitory elements such as bubbles attached to the inlet of the pressure gauge or overflowed to the outside. The advantage is that it obtains a convenient incubator for use in rural areas.

Claims (1)

유리 또는 투명 합성수지제로 성형된 원통형 용기로 되고 상단에 물주입구(105)와 원료투입구(1b)가 있는 덮개(1a)가 장치되며 하단에 배출구(1c)가 구비되어 외통(2)내에 장치되는 배양조(1)와 Cultivation is formed in a cylindrical container formed of glass or transparent synthetic resin, the cover (1a) having a water inlet 105 and the raw material inlet (1b) at the top and an outlet (1c) at the bottom is provided in the outer cylinder (2) Joe (1) and 배양조 외면에 일정간격을 두고 가열실(200)이 형성되게 상부에 덮개(2a)가 덮혀지고 바닥이 막혀지며 바닥일측에서 배기팬(204)이 장치된 외통체(2)와 Covered with a cover (2a) and the bottom is clogged at a predetermined interval on the outer surface of the culture tank to form a heating chamber 200 and the outer cylinder (2) equipped with an exhaust fan 204 on the bottom side; 배양조(1) 바닥 내측으로 장치되어 배양조내에 일정량의 공기를 주입하는 공기공급기(100)와 The air supply unit 100 is installed inside the bottom of the culture tank 1 to inject a predetermined amount of air into the culture tank. 배양조내부에 설치되어 배양조의 온도를 감지하는 온도센서(101)와 Installed in the culture tank and the temperature sensor 101 for sensing the temperature of the culture tank and 배양조 주벽 상단부에서 내측으로 돌출되는 거품제거용 돌기(102)와 Defoaming projection 102 and protrudes inward from the upper end of the culture vessel main wall 배양조 덮개에 장치된 전동기(103a)의 구동축에 연결되어 배양조 위치하는 전동교반기(103)와 An electric stirrer 103 connected to the drive shaft of the electric motor 103a installed in the culture tank cover and positioned in the culture tank; 배양조 덮개에 장치되어 배양조내부의 압력을 표시하는 압력계(104)와 A pressure gauge 104 mounted on the cover of the culture tank to display the pressure inside the culture tank; 외통체(2) 주벽에는 가열실 내부로 향해 장치되어 배양조에 열기를 공급하는 백열전등(201)과 외통체 주벽에서 가열실내부로 향해 장치되어 배양조에 빛을 공급하는 형광등(202)과 외통체 주벽에서 가열실 내부로 향해 장치되어, 배양조로 조명되는 자외선 살균등(203)과 상기 기구들을 적기에 작동시키고 끌 수 있게 구성된 스위치와 장시간 타이머가 구비된 전기회로로된 제어기(3)로 이루어지는 미생물 배양기The outer wall of the outer cylinder 2 has an incandescent lamp 201 that is installed toward the inside of the heating chamber to supply heat to the culture chamber and a fluorescent lamp 202 that is installed from the outer wall of the outer cylinder to the inside of the heating chamber to supply light to the culture chamber and the outer cylinder main wall. Microorganism incubator consisting of an ultraviolet circuit sterilizer 203, which is directed toward the interior of the heating chamber, which is illuminated by a culture tank, an electric circuit controller 3 equipped with a switch configured to timely operate and turn off the apparatus and a long time timer.
KR1020040040438A 2004-06-03 2004-06-03 Microorganism medium KR100604095B1 (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
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KR100930596B1 (en) 2009-05-07 2009-12-09 주식회사 쉴드텍 Integrated cultivating device for proliferation and supply of useful microorganism
KR101149712B1 (en) * 2011-01-31 2012-06-08 사천시 Using method of multi-propose useful micro organism incubator
KR101356722B1 (en) 2012-02-13 2014-02-03 주식회사 지엘바이오 Using Method of Multi-Propose Useful Micro Organism Incubator
KR101374059B1 (en) 2013-08-12 2014-03-25 장충남 Indirect cooling microbial incubator
WO2015041385A1 (en) * 2013-09-23 2015-03-26 주식회사 두산에코비즈넷 Incubator having ultraviolet lamp

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KR101718479B1 (en) * 2014-11-11 2017-03-21 주식회사 월드바이오텍 Fermenter having part for removing bubbles
KR101661480B1 (en) * 2015-03-26 2016-09-30 주식회사 이시스 Apparatus for cultivating microbe
CN108753579A (en) * 2018-07-11 2018-11-06 淮阴师范学院 A kind of microbe soil restoration agent preparation method and its equipment

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100930596B1 (en) 2009-05-07 2009-12-09 주식회사 쉴드텍 Integrated cultivating device for proliferation and supply of useful microorganism
KR101149712B1 (en) * 2011-01-31 2012-06-08 사천시 Using method of multi-propose useful micro organism incubator
KR101356722B1 (en) 2012-02-13 2014-02-03 주식회사 지엘바이오 Using Method of Multi-Propose Useful Micro Organism Incubator
KR101374059B1 (en) 2013-08-12 2014-03-25 장충남 Indirect cooling microbial incubator
WO2015041385A1 (en) * 2013-09-23 2015-03-26 주식회사 두산에코비즈넷 Incubator having ultraviolet lamp

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