KR20050115123A - Microorganism medium - Google Patents

Microorganism medium Download PDF

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Publication number
KR20050115123A
KR20050115123A KR1020040040438A KR20040040438A KR20050115123A KR 20050115123 A KR20050115123 A KR 20050115123A KR 1020040040438 A KR1020040040438 A KR 1020040040438A KR 20040040438 A KR20040040438 A KR 20040040438A KR 20050115123 A KR20050115123 A KR 20050115123A
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South Korea
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culture tank
culture
outer cylinder
heat
lamp
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KR1020040040438A
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Korean (ko)
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KR100604095B1 (en
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유화성
천종구
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유화성
천종구
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
    • C12M1/06—Apparatus for enzymology or microbiology with gas introduction means with agitator, e.g. impeller
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20—Degassing; Venting; Bubble traps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

Abstract

본 발명은 각종 미생물을 편리하게 배양시킬 수 있는 미생물 배양기에 관한 것인데 보다 구체적인 것은 배양조 외면에 가열실과 광원부를 구비하여 가열등과 형광등과 배기팬을 설치하고 배양조 내부에 온도감지센서와 공기공급기와 교반기를 설치하면서 거품제거용 돌기를 배치하여 이들을 전동운전장치와 결합시켜서 운용할 수 있게 하므로서 각종 미생물을 편리하게 배양시킬 수 있게 한 것이다.The present invention relates to a microbial incubator capable of conveniently cultivating various microorganisms, and more specifically, a heating chamber and a light source unit are installed on the outer surface of the culture tank to install a heating lamp, a fluorescent lamp and an exhaust fan, and a temperature sensor and an air supply inside the culture tank. By installing the agitator and the bubble removing projections to be able to operate them in combination with the electric drive device to be able to conveniently culture various microorganisms.

즉, 유리 또는 투명 합성수지제로 성형된 원통형 용기로 된 배양조(1)와 배양조 외면에 일정간격을 두고 피복되어 배양조에서 요구하는 열기와 광원을 공급하고 열기를 조정할 수 있게 배기팬(204)을 구비하는 가열실(200)을 가진 외통체(2)로 이루어져 배양조(1)와 외통체(2)에는 각각 덮개(1a)(2a)와 지지각(2b)이 구비되고 배양조 바닥에는 일정한 량의 공기를 주입하는 공기 공급기(100)와 배양조내의 필요온도를 감지하는 온도센서(101)가 장치되며 주벽 상단에 거품제거용 돌기(102)가 부착되고 전동교반기(103)가 장치되어 전동기(103a)로 관리되며 배양조 덮개에는 압력계(104)와 물주입구(105)와 원료투입구(1b)가 구비되고 외통체(2)에는 내부에 배양조에 열기를 공급하는 백열전등(201)과 배양조에 빛을 공급하는 형광등(202)과 배양액을 살균시킬때 사용하는 자외선 살균등(203)이 장치되고 이러한 기구들을 적기에 작동시키고 끌 수 있게 스위치와 장시간 타이머가 구비된 전기회로로된 제어기(3)가 구비되어서 이루어지는 미생물 배양기이다.That is, the culture tank 1 made of glass or transparent synthetic resin and the exhaust fan 204 are coated at regular intervals on the outer surface of the culture tank so that the heat and light source required by the culture tank can be supplied and the heat can be adjusted. It consists of an outer cylinder (2) having a heating chamber (200) having a chamber (1) and the outer cylinder (2) is provided with a cover (1a) (2a) and a support angle (2b), respectively, An air supply unit 100 for injecting a certain amount of air and a temperature sensor 101 for detecting a required temperature in the culture tank are installed, and a bubble removing protrusion 102 is attached to the top of the main wall, and an electric stirrer 103 is installed. It is managed by an electric motor (103a), the culture tank cover is provided with a pressure gauge 104, a water inlet 105 and a raw material inlet (1b), and the outer cylinder (2) and the incandescent lamp (201) for supplying heat to the culture tank therein and Fluorescent lamp 202 for supplying light to the culture tank and ultraviolet light used to sterilize the culture solution A sterilizing lamp 203, the device being provided with such a mechanism be able to work and turn off the switch at the right time with the controller 3 to the electrical circuit having a long time, timer microorganism culture medium comprising.

Description

미생물 배양기{MICROORGANISM MEDIUM} Microbial incubator {MICROORGANISM MEDIUM}

본 발명은 각종 미생물을 편리하게 배양시킬 수 있는 배양기에 관한 것인데 보다 구체적인 것은 배양조 외면에 가열실과 광원부를 구비하여 가열등과 형광등과 배기팬을 설치하고 배양조 내부에 온도감지센서와 공기공급기와 교반기를 설치하면서 거품제거용 돌기를 배치하여 이들을 전동운전장치와 결합시켜서 관리할 수 있게 제어기를 구비하므로서 각종 미생물을 편리하게 배양시킬 수 있게 한 것이다.The present invention relates to an incubator capable of cultivating various microorganisms conveniently. More specifically, a heating chamber and a light source unit are provided on the outer surface of the culture tank to install a heating lamp, a fluorescent lamp, and an exhaust fan, and a temperature sensor and an air supply unit in the culture tank. By installing a stirrer to arrange the defoaming projections by combining them with the electric drive device to provide a controller that can be managed to conveniently cultivate various microorganisms.

최근에는 다양한 미생물을 농업이나 축산에 많이 이용하고 있고 농업이나 축산의 경우 선택한 미생물을 배양하여 물에 희석시킨다음 토양에 뿌리거나 가축에게 먹이는 방법으로 작물의 생산을 촉진시키고 병충해를 방지하며 가축의 경우 성장을 촉진시키고 질병을 예방하게 되고 육질을 좋게 하며 배설물의 약취와 오염을 줄일 수 있게 되는 효과를 얻고 있다.Recently, various microorganisms are used in agriculture or livestock raising, and in agriculture or livestock raising, the selected microorganisms are cultivated and diluted in water, then sprinkled on soil or fed to livestock to promote crop production and prevent pests. It has been shown to promote growth, prevent disease, improve meat quality, and reduce fecal intoxication and pollution.

따라서 이러한 미생물을 일정한 영양분과 온도와 빛을 이용하여 배양시키는 것이므로 장시간에 걸친 관리를 요하게 되고 이를 위해 카비넷 형상으로된 배양기가 주로 사용되고 있다.Therefore, since the microorganisms are cultured using a constant nutrient, temperature and light, it requires management for a long time and a cabinet-shaped incubator is mainly used for this.

그러나 일손이 바쁜 농촌에서는 이러한 조건을 만족시키는 것이 불가능하므로 만족스러운 효과를 얻지 못하는 실정에 있다.However, it is not possible to satisfy these conditions in a busy rural village, so it is impossible to obtain a satisfactory effect.

본 발명은 상기와 같은 종래의 결점을 시정하여 농촌에서 보다 편리하게 사용할 수 있고 배양효과를 높힐수 있는 안정된 배양기를 제공하는 것이다. The present invention is to provide a stable incubator that can be used more conveniently in rural areas and to improve the culture effect by correcting the above conventional drawbacks.

이를 보다 상세히 첨부된 도면에 의거 상세히 설명하면 다음과 같다. When described in detail based on the accompanying drawings in more detail as follows.

본 발명은 유리 또는 투명 합성수지제로 성형된 원통형 용기로 된 배양조(1)와 배양조 외면에 일정간격을 두고 피복되어 배양조에 필요한 열기와 광원과 열기를 조정하는 배기팬을 구비하는 외통체(2)로 이루어져 배양조(1)와 외통체(2)에는 각각 덮개(1a)(2a)가 씌워지고 배양조 바닥에는 일정한 량의 공기를 주입하는 공기 공급기(100)와 배양조내의 필요온도를 감지하는 온도센서(101)가 장치되며 주벽 상단에 거품제거용 돌기(102)가 부착되고 전동교반기(103)가 장치되어 전동기(103a)로 관리되며 배양조 덮개에는 압력계(104)와 물주입구(105)와 원료투입구(1b)가 구비되고 외통체(2)에는 내부에 배양조에 열기를 공급하는 백열전등(201)과 배양조에 빛을 공급하는 형광등(202)과 배양액을 살균시킬때 사용하는 자외선 살균등(203)이 장치되고 백열등을 비롯하여 살균등과 형광등 그리고 교반기, 공기공급기, 냉각팬을 적기에 작동시키고 끌 수 있게 각종 스위치와 장시간 타이머가 구비된 전기회로로 구성된 제어기(3)가 구비되어서 이루어지는 미생물 배양기이다.The present invention is a cylindrical tank formed of glass or transparent synthetic resin (1) and an outer cylinder (2) provided with a predetermined interval on the outer surface of the culture tank having a heat source and a light source and the exhaust fan for adjusting the heat (2) The cover 1a and 2a are respectively attached to the culture tank 1 and the outer cylinder 2, and the air supply 100 for injecting a certain amount of air into the culture tank bottom and senses the necessary temperature in the culture tank. The temperature sensor 101 is provided and the bubble removing projection 102 is attached to the top of the main wall and the electric stirrer 103 is installed and managed by the electric motor 103a. The pressure gauge 104 and the water inlet 105 are provided on the culture tank cover. ) And a raw material inlet (1b) and the outer cylinder (2) is an ultraviolet sterilization used to sterilize the incandescent lamp (201) for supplying heat to the culture tank and the fluorescent lamp (202) for supplying light to the culture tank and the culture solution The lamp 203 is equipped and live including an incandescent lamp As a fluorescent lamp and a stirrer, the air supply, being provided to operate the cooling fan in a timely manner, and can make various switches and the controller (3) consisting of an electrical circuit with a long time, timer off microorganism culture medium comprising.

이와같이된 본 발명은 배양조(1)내에 배양액을 넣고 선택된 미생물을 주입하여 배양을 시키게 되는데 (배양액은 질소, 인산, 카리, 규산, 칼슘, 마그네슘, 망간, 붕소, 철, 몰리브덴, 아연, 구리, 한천, 혼합물로 조성된 유기물 배지 5kg에 물200ℓ를 주입하는 비율로 배양액을 만들어 미생물 200g를 넣는 비율로 조성함) 스위치SW-1을 ON하면 전원이 공급되면서 10시간에서 300 시간용 타이머1 이 동작하여 릴레이 1접점을 통해 타이머2에 전원이 공급되고 릴레이 2에 의하여 자외선 살균등(203)이 점등되어 배양액을 살균하게 되며 타이머 2에 설정된 시간이 경과되면 릴레이 2가 복귀되어 살균등은 꺼지고 복귀접점에 의하여 삼파장, 형광등, 교반기, 기포발생기, 온도감지기 및 부저에 전원이 공급되어 부저가 눌리면 준비된 미생물 균주를 투입하여 배양을 시작하고 온도감지기의 설정온도에 따라 릴레이3의 동작접점에 의하여 배양액 가열용 백열전등과 냉각팬이 교대로 동작하여 배양액의 온도를 약30도로 유지하여 주며 타이머1의 설정시간이 경과되면 전체전원이 꺼지게 되고 배양이 완성되는 것이다.In the present invention, the culture solution is placed in the culture tank 1 and the selected microorganisms are injected to culture the culture medium (the culture solution is nitrogen, phosphoric acid, carry, silicic acid, calcium, magnesium, manganese, boron, iron, molybdenum, zinc, copper, Agar and make a culture medium at the rate of injecting 200 liters of water into 5kg of organic medium composed of a mixture, and the rate of 200g of microorganisms is added.) When switch SW-1 is turned on, timer 1 operates for 10 to 300 hours while power is supplied. Then, power is supplied to timer 2 through relay 1 contact, and ultraviolet sterilization lamp 203 is turned on by relay 2 to sterilize the culture solution. When the time set in timer 2 has elapsed, relay 2 is restored and the sterilization lamp is turned off. By supplying power to three wavelengths, fluorescent lamps, stirrers, bubble generators, temperature detectors and buzzers, when the buzzer is pressed, the prepared microbial strain is introduced to start cultivation. According to the set temperature of the high temperature sensor, the incandescent lamp for heating the medium and the cooling fan are operated alternately by the operating contact of the relay 3 to maintain the temperature of the medium about 30 degrees. And the culture is completed.

따라서 본 발명은 호기성 균주를 비롯하여 광합성미생물 균주까지 배양을 시킬 수 있도록 되고 10시간에서 300시간 타이머를 채용하여 장시간의 배양온도관리를 달성하게 되며 외통에 장치되는 백열등과 형광등 및 자외선 살균등은 교체가 용이하므로 고장시 신속한 수리를 통해 지속적인 배양을 달성하게 된다.Therefore, the present invention is capable of culturing even aerobic strains and photosynthetic microorganism strains, and employing a timer for 10 hours to 300 hours to achieve long-term culture temperature management, and replace incandescent lamps, fluorescent lamps, and ultraviolet sterilization lamps installed in external cylinders. It is easy to achieve continuous culture through rapid repair in case of failure.

또 본 발명의 배양조에는 상단부 주벽내측에 거품제거용 돌기(102)가 설치되어 있으므로 배양중에 발생하는 거품을 제거하여 거품이 압력계의 입구에 부착하거나 외부로 넘치는 등으로 배양저해요소를 해소시킬 수 있는 이점이 있어서 농촌에서 사용하기에 편리한 배양기를 얻는 것이다.In addition, the culture tank of the present invention is provided with a bubble removing projection 102 in the inner peripheral wall of the upper end to remove the bubbles generated during the culture to eliminate the culture inhibitory elements such as bubbles attached to the inlet of the pressure gauge or overflowed to the outside. The advantage is that it obtains a convenient incubator for use in rural areas.

도 1은 본 발명의 외관 사시도로서 덮개를 분리한 예시도1 is an exploded perspective view of the present invention showing the cover removed

도 2는 본 발명의 단면도2 is a cross-sectional view of the present invention

도 3은 본 발명의 또 다른 실시상태를 보이는 분해 사시도Figure 3 is an exploded perspective view showing another embodiment of the present invention

도 4는 도3의 단면도4 is a cross-sectional view of FIG.

도 5는 본 발명의 횡단 평면도5 is a cross-sectional plan view of the present invention.

도 6은 본 발명의 작동 회로예시도이다.6 is an exemplary operation circuit diagram of the present invention.

* 도면의 주요부분에 대한 부호설명* Explanation of symbols on the main parts of the drawings

1 : 배양조 1b : 원료투입구1: culture tank 1b: raw material inlet

1c : 배출구 2 : 외통체1c: outlet 2: outer cylinder

3 : 제어기 100 : 공기공급기3: controller 100: air supply

101 : 온도센서 102 : 거품제거용돌기101: temperature sensor 102: bubble removing projection

103 : 전동교반기 104 : 압력계103: electric stirrer 104: pressure gauge

105 : 물주입구 201 : 백열전등105: water inlet 201: incandescent lamp

202 : 형광등 203 : 살균등202: fluorescent lamp 203: germicidal lamp

Claims (1)

유리 또는 투명 합성수지제로 성형된 원통형 용기로 된 배양조(1)와 배양조 외면에 일정간격을 두고 피복되어 배양조에서 요구하는 열기와 광원을 공급하고 열기를 조정할 수 있게 배기팬(204)을 구비하는 가열실(200)을 가진 외통체(2)로 이루어져 배양조(1)와 외통체(2)에는 각각 덮개(1a)(2a)와 지지각(2b)이 구비되고 배양조 바닥에는 일정한 량의 공기를 주입하는 공기 공급기(100)와 배양조내의 필요온도를 감지하는 온도센서(101)가 장치되며 주벽 상단에 거품제거용 돌기(102)가 부착되고 전동교반기(103)가 장치되어 전동기(103a)로 관리되며 배양조 덮개에는 압력계(104)와 물주입구(105)와 원료투입구(1b)가 구비되고 외통체(2)에는 내부에 배양조에 열기를 공급하는 백열전등(201)과 배양조에 빛을 공급하는 형광등(202)과 배양액을 살균시킬때 사용하는 자외선 살균등(203)이 장치되고 이러한 기구들을 적기에 작동시키고 끌 수 있게 스위치와 장시간 타이머가 구비된 전기회로로된 제어기(3)가 구비되어서 이루어지는 미생물 배양기A culture tank 1 formed of a glass container or a cylindrical container made of glass or transparent synthetic resin and an exhaust fan 204 are coated with a predetermined interval on the outer surface of the culture tank so as to supply the heat and light source required by the culture tank and to adjust the heat. Comprising an outer cylinder (2) having a heating chamber 200 to the culture tank (1) and the outer cylinder (2) is provided with a cover (1a) (2a) and a support angle (2b), respectively, a constant amount at the bottom of the culture tank The air supply 100 for injecting air and the temperature sensor 101 for detecting the required temperature in the culture vessel is equipped with a bubble removing projection 102 is attached to the top of the main wall and the electric stirrer 103 is equipped with an electric motor ( 103a), the culture tank cover is provided with a pressure gauge 104, a water inlet 105 and a raw material inlet (1b) and the outer cylinder (2) to the incandescent lamp 201 and the culture tank to supply heat to the culture tank therein Fluorescent lamp 202 to supply light and ultraviolet rays used to sterilize the culture medium Equal 203 device is such a mechanism be provided to operate them at the right time and can allow the switch and the controller 3 to the electrical circuit provided with a timer to turn off for a long time comprising microbial incubator
KR1020040040438A 2004-06-03 2004-06-03 Microorganism medium KR100604095B1 (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20160056105A (en) * 2014-11-11 2016-05-19 주식회사 월드바이오텍 Fermenter having part for removing bubbles
KR101661480B1 (en) * 2015-03-26 2016-09-30 주식회사 이시스 Apparatus for cultivating microbe
CN108753579A (en) * 2018-07-11 2018-11-06 淮阴师范学院 A kind of microbe soil restoration agent preparation method and its equipment

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KR100930596B1 (en) 2009-05-07 2009-12-09 주식회사 쉴드텍 Integrated cultivating device for proliferation and supply of useful microorganism
KR101149712B1 (en) * 2011-01-31 2012-06-08 사천시 Using method of multi-propose useful micro organism incubator
KR101356722B1 (en) 2012-02-13 2014-02-03 주식회사 지엘바이오 Using Method of Multi-Propose Useful Micro Organism Incubator
KR101374059B1 (en) 2013-08-12 2014-03-25 장충남 Indirect cooling microbial incubator
KR101537379B1 (en) * 2013-09-23 2015-07-16 주식회사 두산에코비즈넷 Culture device with uv lamp

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Publication number Priority date Publication date Assignee Title
KR20160056105A (en) * 2014-11-11 2016-05-19 주식회사 월드바이오텍 Fermenter having part for removing bubbles
KR101661480B1 (en) * 2015-03-26 2016-09-30 주식회사 이시스 Apparatus for cultivating microbe
CN108753579A (en) * 2018-07-11 2018-11-06 淮阴师范学院 A kind of microbe soil restoration agent preparation method and its equipment

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