KR200485345Y1 - Microorganisms incubating tank - Google Patents

Microorganisms incubating tank Download PDF

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Publication number
KR200485345Y1
KR200485345Y1 KR2020150008226U KR20150008226U KR200485345Y1 KR 200485345 Y1 KR200485345 Y1 KR 200485345Y1 KR 2020150008226 U KR2020150008226 U KR 2020150008226U KR 20150008226 U KR20150008226 U KR 20150008226U KR 200485345 Y1 KR200485345 Y1 KR 200485345Y1
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KR
South Korea
Prior art keywords
culture
tank
supply pipe
circulation tube
pipe
Prior art date
Application number
KR2020150008226U
Other languages
Korean (ko)
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KR20170002221U (en
Inventor
김진호
Original Assignee
(주)한성플랜트
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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Priority to KR2020150008226U priority Critical patent/KR200485345Y1/en
Publication of KR20170002221U publication Critical patent/KR20170002221U/en
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Publication of KR200485345Y1 publication Critical patent/KR200485345Y1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C12M27/04—Stirrer or mobile mixing elements with introduction of gas through the stirrer or mixing element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

Abstract

The present invention relates to a microorganism culture tank capable of regulating the culture temperature in a culture tank by circulating a culture liquid, comprising a tank in which a culture chamber for storing a culture liquid is formed, and a culture solution circulation tube provided at one side of the tank so that both ends communicate with the culture chamber A heating device provided at one side of the culture circulation tube for heating the culture solution circulating through the culture solution circulation tube and an air supply tube formed at the other side of the culture solution circulation tube and supplying air to the culture chamber through the culture solution circulation tube .

Description

Microorganisms incubating tank < RTI ID = 0.0 >

The present invention relates to a microorganism culture tank, and relates to a microorganism culture tank capable of controlling a culture temperature in a culture tank by circulating a culture liquid.

In general, microorganisms are widely used in agriculture, animal husbandry, and fisheries. As a method of using these microorganisms, photosynthesis bacteria, lactic acid bacteria, Bacillus bacteria, homocysteine and southern bacteria are cultured and diluted in water, Spraying, crossing crops, or foliar spraying or fed to livestock.

These microorganisms can promote the production of crops and prevent pests and diseases. In the case of livestock, it promotes growth, prevents disease, improves meat quality, reduces odor and pollution of excretion, Inhibiting or benefiting beneficial bacteria and inhibiting harmful bacteria.

In addition, microorganisms can be used for purification of water, purification of organic wastes, purification of various industrial wastes, and purification of water quality, inhibition of generation of pathogenic bacteria, and improvement of disease resistance when used in aquaculture.

Since these microorganisms are cultured using light with a certain nutrient, temperature, and occasionally, conventional microorganism incubators use a large number of heat lamps and the like for a long time in order to satisfy a light source. In this case, microorganisms are cultured for a long time The light source is satisfied by the irradiation lamp such as the heat lamp, but the temperature is continuously increased, and the control to the proper temperature is not smooth.

Further, when a separate heater is provided on the inner side of the culture tank, there is a problem that it is difficult to uniformly maintain the temperature in the culture tank, such as a temperature difference between a region adjacent to the heater and a region far from the heater.

In addition, when air is supplied to the inside of the incubation tank by a separate air generator, the supplied air is not uniformly dispersed in the culture tank, so that there is a problem that optimal conditions for culturing the microorganism can not be provided.

It is an object of the present invention to provide a microorganism culture tank capable of uniformly supplying heat required for culturing microorganisms throughout the culture solution.

Another object of the present invention is to provide a microorganism culture tank capable of uniformly supplying air throughout the stored culture liquid.

Another object of the present invention is to provide a microorganism culture tank in which heat and air are uniformly dispersed throughout the culture solution by convection.

The object of the present invention described above is to provide a tank in which a culture chamber for storing a culture liquid is formed; A culture liquid circulation tube installed at one side of the tank so that both ends communicate with the culture chamber; A heating device installed at one side of the culture circulation tube and heating the culture solution circulating through the culture solution circulation tube; And an air supply tube formed on the other side of the culture circulation tube for supplying air to the culture chamber through the circulation tube for circulating the culture solution.

According to a preferred feature of the present invention, the culture circulation tube is installed at the lower side of the outer side of the tank, and the culture liquid in the culture chamber can be heated by convection due to the culture solution re-flowing through the culture circulation tube.

According to another preferred characteristic of the present invention, the culture circulation tube comprises a culture liquid discharge tube having one end communicating with the culture chamber and the other end extending to the outside of the tank, and a heating device provided on one side, And a culture liquid supply pipe spaced from the culture liquid discharge pipe by a predetermined distance and having one end communicating with the connection pipe and the other end communicating with the culture chamber.

According to another preferred aspect of the present invention, the heating device may include a coupling portion coupled to one side of the coupling tube, and a heating portion inserted into the coupling tube.

According to another preferred aspect of the present invention, one end of the air supply pipe may be inserted into the connection pipe and extend to one end of the culture solution supply pipe.

According to another preferred feature of the present invention, the inner diameter of the culture liquid supply pipe is larger than the outer diameter of the air supply pipe, and the culture liquid of the connection pipe flows into the culture liquid supply pipe through a gap formed between the culture liquid supply pipe and the air supply pipe .

According to another preferred aspect of the present invention, a temperature sensor provided at one side of the culture circulation tube and measuring the temperature of the culture liquid, and an air blower provided at one side of the air supply tube and supplying air thereto .

According to another preferred feature of the present invention, there is further provided a control panel installed at one side of the tank and controlling the operation of the air blower and the heating device, wherein the operation of the heating device according to the value detected from the temperature sensor Can be controlled.

According to the microorganism culture tank according to the present invention, the temperature in the culture tank can be maintained at a proper temperature for culturing the microorganism by supplying heat during circulation of the stored culture liquid.

In addition, according to the microorganism culture tank according to the present invention, since the culture liquid in the culture chamber is circulated together with the air supply, there is no need for a separate pump device or agitator for circulating the culture liquid, thereby reducing installation and operation costs.

In addition, according to the microorganism culture tank according to the present invention, since the culture liquid heated in the circulation process is injected into the culture tank together with air, heat and air can be uniformly distributed and supplied to the entire culture tank.

Further, according to the microorganism culture tank according to the present invention, heat and air are uniformly supplied to the entire culture tank by the convection phenomenon.

In addition, according to the microorganism culture tank according to the present invention, since the heater is installed in the incubation tank as in the prior art, the temperature difference generated in the region adjacent to the heater and in the region far from the heater can be reduced.

In addition, according to the microorganism culture tank according to the present invention, it is possible to prevent the problem that the microorganisms in the vicinity of the heater are destroyed by the high temperature due to the heater installed in the conventional culture tank, so that the growth of microorganisms and the commerciality are greatly reduced.

1 is a front view of a microorganism culture tank according to one embodiment of the present invention.
2 is an enlarged view of a portion "A"
3 is a side view of a microorganism culture tank according to one embodiment of the present invention;
FIG. 4 is a plan view of a microorganism culture tank according to an embodiment of the present invention; FIG.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. It should be understood, however, that the embodiments described below are only for the purpose of illustrating the invention in a manner that allows a person skilled in the art to easily implement the invention, It does not mean anything. In describing the embodiments of the present invention, the same reference numerals are used for the components having the same technical characteristics.

Example

FIG. 1 is a front view of a microorganism culture tank according to one embodiment of the present invention, and FIG. 2 is an enlarged view of a portion "A" of FIG. FIG. 3 is a side view of a microorganism culture tank according to an embodiment of the present invention, and FIG. 4 is a plan view of a microorganism culture tank according to an embodiment of the present invention.

1 to 4, the microorganism culture tank 100 according to an embodiment of the present invention includes a tank 200 in which a culture medium is stored, a culture medium circulation tube 200 installed at one side of the tank 200, A heating device 400 installed on one side of the culture circulation pipe 300 and an air supply pipe 500 installed on the other side of the culture solution circulation pipe 300.

The culture chamber in which the culture liquid is stored is formed inside the tank 200, and it is preferable that the culture chamber is manufactured in consideration of heat insulation. As an example, the outer wall of the tank 200 may have a double structure for improving the heat insulating property.

The tank 200 may be spaced apart from the ground by a predetermined height. For this purpose, a support part 600 composed of a plurality of frames may be formed to support the bottom surface of the tank 200.

The culture liquid circulating tube 300 is installed at the lower end of the tank 200. The culture liquid stored in the culture chamber is discharged to the outside of the culture chamber through the circulation tube 300 and returned to the culture chamber. According to one embodiment of the present invention, heat is supplied during the circulation of the culture liquid. Therefore, the culture liquid discharged from the culture chamber is heated to a higher temperature than that discharged from the culture circulation tube 300, and then flows into the culture chamber again.

The culture circulation tube 300 is shaped like a hollow pipe and protrudes to the outside of the tank 200. Both ends of the culture circulation tube 300 are installed so as to communicate with the culture chamber inside the tank 200. The culture circulation tube 300 includes a culture solution discharge tube 310 formed to discharge the culture solution stored in the culture chamber to the outside of the tank 200, a connection tube 320 configured to communicate with the end of the culture solution discharge tube 310, And a culture solution supply pipe 330 extending from the connection pipe 320 to communicate with the culture room again and formed alongside the culture solution discharge pipe 310.

According to an embodiment of the present invention, the culture liquid discharge pipe 310 is connected to the culture chamber at one end and extends outwardly of the tank 200 and then vertically downwardly bent to extend upwardly of the connection pipe 320. The culture liquid supply pipe 330 is extended from the upper side of the connection pipe 320 to the outer side of the tank 200 while being spaced apart from the culture liquid discharge pipe 310 by a distance from the culture liquid discharge pipe 310 and the connection pipe 320 . At this time, the connection pipe 320 and the culture solution supply pipe 330 communicate with each other, and the culture solution supply pipe 330 extends vertically from the upper side of the connection pipe 320 and then is bent in the direction of the tank 200 to communicate with the culture chamber .

A connection pipe 320 is formed at the lower end of the culture solution discharge pipe 310 and the culture solution discharge pipe 310 and the connection pipe 320 communicate with each other. Accordingly, the culture solution discharged to the outside of the culture chamber through the culture solution discharge pipe 310 flows into the connection pipe 320. In the embodiment shown in the drawing, the connecting pipe 320 is shown as a square pipe, but this is merely one embodiment of the present invention, and the connecting pipe 320 is formed of pipes having various sectional shapes such as a circular shape or a polygonal shape . The connection pipe 320 may be supported by a bracket 610 coupled to one side of the support part 600.

A heating device 400 is provided on one side of the connection pipe 320. The heating device 400 is for heating the culture liquid discharged through the culture solution discharge pipe 310 and flowing into the connection pipe 320. The heating device 400 includes a heating part 410 inserted into the connection pipe 320, And a coupling portion 420 provided at one side of the coupling pipe 410 and coupled to one side of the coupling pipe 320.

At this time, the heating unit 410 may be a heating coil that generates a resistance heat by an external power source. The culture liquid flowing into the connection pipe 320 through the culture medium discharge pipe 310 is heated by the heating unit 410 to be heated.

An air supply pipe (500) is formed on the other side of the connection pipe (320). The air supply pipe 500 is for supplying air to the culture chamber. One end of the air supply pipe 500 is inserted into the connection pipe 320, and then extends to one end of the culture solution supply pipe 330. An air blower (not shown) for supplying air is provided at one side of the air supply pipe 500, and the air is supplied to the culture chamber through the air supply pipe 500 and the culture solution supply pipe 330 in order.

Here, the culture liquid supply pipe 330 has an inner diameter larger than the outer diameter of the air supply pipe 500, so that a gap is formed between the outer circumferential surface of the air supply pipe 500 and the inner circumferential surface of the culture liquid supply pipe 330. The air supply pipe 500 injects high-pressure air toward the culture solution supply pipe 330 and the culture solution of the connection pipe 320 flows into the culture solution supply pipe 330 through the gap due to the pressure difference generated at this time. That is, the culture liquid that flows into the connection pipe 320 and is heated by the heating unit 410 is mixed with air through the culture solution supply pipe 330 and re-introduced into the culture chamber.

At this time, a heater (not shown) for raising the supplied air may be further provided at one side of the air supply pipe 500 in order to prevent the temperature of the culture liquid from dropping when the air is mixed with the air. The heater may be formed, for example, so as to surround the outer circumferential surface of one side of the air supply pipe 500, or may be installed to heat air supplied from an air tank (not shown) to the air blower.

As described above, according to one embodiment of the present invention, the culture liquid that has been raised while passing through the culture circulation tube 300 is mixed with the high-pressure air supplied through the air blower and is injected strongly into the culture chamber, The heat of the culture liquid is diffused evenly into the culture chamber by the injection pressure.

At this time, since the culture solution supply pipe 330 is connected to the lower end of the tank 200, the culture solution stored in the culture chamber is circulated in the vertical direction by the convection phenomenon. That is, the heat and air injected into the culture chamber through the culture medium supply pipe 330 are first transferred to the culture solution in the lower portion of the culture chamber, and the culture solution in the lower portion of the culture chamber rises to the upper portion of the culture chamber due to the temperature rise. When the temperature is lowered, the culture liquid descends again to the lower portion of the culture chamber. When the culture liquid descends to the lower portion of the culture chamber, the culture liquid is received again from the culture liquid injected through the culture liquid supply pipe 330, will be.

According to one embodiment of the present invention, since the culture liquid in the culture chamber circulates by the convection phenomenon, it is not necessary to provide a separate stirrer, and the heat and air supplied to the lower portion of the culture chamber are uniformly dispersed throughout the culture chamber. So that it is possible to uniformly maintain the quality.

A control panel 700 for controlling the operation of the air blower may be provided in the inside of the culture circulation pipe 300 and the air supply pipe 500 in order to remove foreign substances from the tank 200. [ Can be provided on one side. Although the control panel 700 is provided at the upper end of the outer circumferential surface of the tank 200 according to the embodiment of the present invention, this is merely an example of the present invention, and the installation position of the control panel 700 may be appropriately adjusted Can be selected.

The heating device 400 may also be controlled by the control panel 700. To this end, the temperature for measuring the temperature of the culture liquid is measured at one side of the culture circulation pipe 300, for example, at one side of the culture solution discharge pipe 310. [ It is preferable that a sensor 800 is provided and ON / OFF or heating temperature control of the heating device 400 can be performed manually or automatically according to the measured value from the temperature sensor 800. At this time, the measurement value of the temperature sensor 800 may be displayed on the display of the control panel 700. If the temperature of the culture liquid is too high, a cooler (not shown) Or it may be installed on one side of the circulation pipe 300 or the air supply pipe 500.

The tank 200 and the culture solution discharge pipe 310, the culture solution discharge pipe 310 and the connection pipe 320, the air supply pipe 500 and the connection pipe 320, the connection pipe 320 and the culture solution supply pipe 330 Welded or joined by separate fasteners such as bolts or nuts. A drain valve 340 may be provided at one side of the culture solution discharge pipe 310 or the culture solution supply pipe 330 to discharge the culture solution in the culture chamber to the outside.

While the present invention has been described in connection with certain exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the scope of the present invention.

100: microorganism culture tank
200: tank
300: Culture circulation tube
310:
320: Connector
330: culture medium supply pipe
400: Heating device
500: air supply pipe
600: Support
700: Control panel
800: Temperature sensor

Claims (8)

A tank in which a culture chamber for storing a culture liquid is formed;
A culture liquid circulation tube installed at one side of the tank so that both ends communicate with the culture chamber;
A heating device installed at one side of the culture circulation tube and heating the culture solution circulating through the culture solution circulation tube;
An air supply pipe formed at the other side of the culture circulation tube and injecting high-pressure air into the culture chamber through the culture circulation tube;
A heater disposed at one side of the air supply pipe for raising the air supplied through the air supply pipe; And
And a cooler installed at one side of the tank for lowering the temperature of the culture liquid,
The culture circulation tube includes a culture liquid discharge pipe having one end communicating with the culture chamber and the other end extending outwardly of the tank and then being bent vertically downwardly, a connection pipe communicating with the other end of the culture liquid discharge pipe, And a culture liquid supply pipe which is formed in parallel with the culture liquid discharge pipe so as to be spaced apart from the culture liquid discharge pipe and extends vertically from the upper side of the connection pipe and is bent in the tank direction and communicates with the culture chamber,
The heating device includes a coupling part coupled to one side of the coupling tube and a heating part inserted into the coupling tube,
Wherein one end of the air supply pipe is inserted into the connection pipe and extends to one end of the culture solution supply pipe, the inner diameter of the culture solution supply pipe is larger than the outer diameter of the air supply pipe, Wherein the microorganism is introduced into the culture medium supply pipe through a gap formed between the supply pipe and the air supply pipe. The method according to claim 1,
Wherein the culture circulation tube is installed at an outer lower side of the tank and the culture solution in the culture chamber is heated by convection due to the culture solution re-flowing through the culture solution circulation tube. delete delete delete delete The method according to claim 1,
A temperature sensor provided at one side of the culture circulation tube and measuring the temperature of the culture solution; and an air blower provided at one side of the air supply pipe and supplying air. The method of claim 7,
Further comprising a control panel installed at one side of the tank and controlling operation of the air blower and the heating device, wherein the operation of the heating device is controlled according to a value detected from the temperature sensor .
KR2020150008226U 2015-12-15 2015-12-15 Microorganisms incubating tank KR200485345Y1 (en)

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KR200485345Y1 true KR200485345Y1 (en) 2017-12-26

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102259510B1 (en) 2020-12-20 2021-06-01 이정복 Microbial cultivation apparatus with independent sterilization and performance testing of microfilter

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102330899B1 (en) * 2021-02-10 2021-11-24 박성일 Compost fermentation apparatus

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101025339B1 (en) * 2010-04-29 2011-03-29 주식회사 글로벌유니언 Microorganism cultivation device
KR101050208B1 (en) 2008-12-30 2011-07-19 주식회사 코넴바이오 Forced Circulation Microbial Fermenter

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR200244404Y1 (en) * 1999-05-10 2001-09-25 정세영 lower part heating water tank
KR101535099B1 (en) * 2013-11-20 2015-07-24 임정식 microorganism culture device with venturi

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101050208B1 (en) 2008-12-30 2011-07-19 주식회사 코넴바이오 Forced Circulation Microbial Fermenter
KR101025339B1 (en) * 2010-04-29 2011-03-29 주식회사 글로벌유니언 Microorganism cultivation device

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102259510B1 (en) 2020-12-20 2021-06-01 이정복 Microbial cultivation apparatus with independent sterilization and performance testing of microfilter

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