KR102259510B1 - Microbial cultivation apparatus with independent sterilization and performance testing of microfilter - Google Patents

Abstract

The present invention relates to a microbial cultivator capable of independently sterilizing an antibacterial filter from the cultivator and testing performance of the same. More specifically, sterilization, cooling, and drying processes of an antibacterial filter can be performed at any time regardless of sterilization and cooling processes of the cultivator, that is a fermentation bath, so that time exposing the antibacterial filter to high temperature by the sterilization process of the cultivator is minimized, thereby significantly increasing performance and use time of the antibacterial filter. The antibacterial filter can be sterilized, cooled, and dried at any time before the sterilization process of microorganisms, so that the performance of the antibacterial filter can be extended, thereby preventing contamination of a culture medium due to degradation of the antibacterial filter. In addition, the performance of the antibacterial filter can be tested at any time, thereby preventing contamination of the culture medium due to deterioration of the antibacterial filter and ensuring replacement of the antibacterial filter at an appropriate time. The microbial cultivator comprises an air inlet, an antibacterial filter unit, a cultivator, a test unit, and a steam supply unit.

Description

Microbial cultivation apparatus with independent sterilization and performance testing of microfilter

The present invention relates to a microorganism incubator capable of independently sterilizing the sterilization filter from the incubator and testing its performance. More specifically, the sterilization of the sterilization filter at any time prior to performing the sterilization and cooling process of the fermenter, that is, the incubator. , cooling, and drying processes can be performed, minimizing the time the sterilization filter is exposed to high temperatures by the sterilization process of the incubator, thereby greatly increasing the performance and use time of the sterilization filter, and prior to performing the sterilization process of the microorganism incubator. Since the sterilization filter can be sterilized, cooled and dried at any time, the performance of the sterilization filter can be extended, so it is possible to prevent contamination of the culture medium due to the deterioration of the sterilization filter, and also to test the performance of the sterilization filter at any time to prevent deterioration of the sterilization filter performance It is a technical field related to a microorganism incubator equipped with independent sterilization of the sterilization filter and the performance test function of the sterilization filter to prevent contamination of the culture medium by contamination and to allow replacement of the sterilization filter at an appropriate time.

In general, microorganisms are widely used in agriculture, livestock, fishery, etc., and methods of using these microorganisms include culturing Bacillus subtilis, actinomycetes, lactic acid bacteria, yeast, etc. to disinfect seeds, spray soil, irrigate crops or foliar spray, and feed livestock. As a method of feeding as a method, in the case of crops, it is used to increase productivity and prevent pests and diseases, as well as to promote growth and prevent diseases in the case of livestock, while obtaining an excellent effect of meat quality.

In addition, microorganisms are widely used in the environmental field for water purification, fermenting agents for organic wastes, and purification of various industrial wastes. When used in aquaculture, it is possible to obtain effects such as water purification, suppression of the generation of pathogens, and improvement of disease resistance. It is favored as a fermenting agent for food waste and an additive for feed in the livestock sector.

Recently, the demand for microorganisms in the environment and livestock fields is rapidly increasing, and accordingly, research and development of devices and methods for mass production by artificially pure culture of microorganisms are being actively conducted. ㆍA culture environment such as air should be created.

In the past, various microorganism incubators have been proposed for mass production by culturing pure microorganisms, and as a representative example of this, a culture medium (glucose, soy flour, microelements, etc.) is introduced into a culture tank equipped with a certain space, The culture medium is sterilized and cooled by applying heat while agitating the culture medium with agitating blades rotated by the drive of a motor, and then inoculated with a seed and stirred and fermented in the culture medium, thereby proliferating a large amount of microorganisms and pure culture.

On the other hand, in general, an incubator, that is, a fermenter, is a device for purely culturing microorganisms or culturing microorganisms to obtain useful substances. Because it is very important, ventilation of the sterilization process and sterilization air becomes a very important task, and samples are frequently collected to measure and evaluate the presence of contamination and the degree of culture.

Such a fermenter includes an aeration device for introducing sterilization air into the vessel by a vessel and a control device for fermenting and culturing the microbial spawn together with the medium, a sterilization filter for sterilizing the air introduced through the aeration device, and the vessel; A steam inlet pipe for sterilizing the medium input to the sterilization or sterilizing the filter for sterilizing the air flowing into the vessel, and a sampling port for sampling and collecting microorganisms during fermentation and culturing on one side of the lower side of the vessel, and the fermentation at the lower side of the vessel and a harvest port for discharging the cultured microorganisms.

In addition, in the case of culturing aerobic microorganisms in the fermenter as described above, in addition to the sterilization process, the air introduced through the vent pipe must be sterilized by the sterilization filter and supplied to the vessel. It is very important for culture.

However, in the conventional fermenter, steam is supplied through the sterilization filter during the sterilization process by steam, so there is a problem in that the performance of the sterilization filter is deteriorated. There is a problem in that the culture medium is contaminated and thus all of the culture medium inside the vessel must be discarded.

Republic of Korea Registered Utility Model No. 20-0485345 (Notice 2017.12.19.) Republic of Korea Registered Utility Model No. 10-0972755 (Announcement 2010.07.22.) Republic of Korea Patent Publication No. 10-1137507 (Announcement 2012.04.20.) Republic of Korea Patent Publication No. 10-0949082 (Announcement 2010.03.22.)

The present invention is a technology devised to solve the problems according to the above-described prior art, and in the conventional microbial incubator, the sterilization filter is exposed to high temperature during the sterilization process by steam during the sterilization, cooling, and drying process of the sterilization filter. As the performance of the sterilization filter deteriorates and the performance of the sterilization filter that sterilizes the air supplied to the incubator cannot be tested frequently, it is difficult to understand the proper replacement cycle of the sterilization filter, and the performance of the sterilization filter is reduced accordingly. Due to this contamination problem;

As a solution to this, the sterilization, cooling and drying process of the sterilization filter can be performed at any time before the sterilization, cooling and drying process of the incubator, that is, the incubator, as well as the sterilization, cooling and drying process of the sterilization filter at any time before sterilization of the incubator of microorganisms A test container is attached and detached so that the performance of the sterilization filter can be tested at any time during the incubation period to prevent contamination of the culture medium due to deterioration of sterilization filter performance and at the same time to ensure that the sterilization filter can be replaced at an appropriate time. Its main purpose is to provide through a microorganism incubator equipped with independent sterilization of the filter and performance test function of the sterilization filter.

The present invention provides an air inlet unit 100 for introducing air; and a sterilization filter unit 200 for sterilizing air introduced from the air inlet unit 100; and the sterilization filter. an incubator 300 receiving the sterilized air from the unit 200; and a test unit 400 branched and connected to the air inlet unit 100 to receive the sterilization air that has passed through the sterilization filter unit 200 and perform a performance test; And the steam is supplied to the air inlet 100, but is supplied to one side of the air inlet 100 before passing through the sterilization filter unit 200 or the other side of the air inlet 100 after passing through the sterilization filter unit 200 It is characterized in that it comprises a; steam supply unit 500 for supplying to the.

The air inlet 100 includes an air inlet pipe 110 for introducing air; and a ventilation inlet valve 120 installed at the other end of the air inlet pipe 110; and a main vent pipe 130 having one end installed on the vent inlet valve 120. A microorganism incubator having a function of independent sterilization of a sterilization filter and a performance test function of the sterilization filter, characterized in that it comprises a.

The sterilization filter unit 200 sterilizes the incoming air. The housing 210 is installed in the main vent pipe 130 and has a sterilization filter installed therein; and one end is connected to the lower part of the housing 210 . , a lower pipe 220 to which the lower discharge valve 222 is installed; and an upper pipe 230 having one end connected to the upper portion of the housing 210 and having an upper discharge valve 232 installed therein; and a pressure gauge 240 connected to the housing 210 to measure the pressure inside the housing 210 .

The air inlet 100 is a main vent discharge valve 140 installed on the other side of the main vent pipe 130; and the other side of the main vent discharge valve 140, which is installed on the other side of the main vent pipe 130. The sub vent discharge valve 150 is installed to be located in; and one end is connected to the other side of the main vent pipe 130, and one end is connected between the main vent discharge valve 140 and the sub vent discharge valve 150. Connected ventilation branch pipe (160); and a second ventilation discharge valve 180 installed on the other side of the ventilation branch pipe 160 and installed on the other side of the first ventilation discharge valve 170 .

The steam supply unit 500 has the other end connected to the other side of the main vent pipe 130, the main steam supply pipe 510 for supplying steam by being connected to the other end of the sub vent discharge valve 150 so that the other end is located; and A main steam supply valve 520 installed on one side of the main steam supply pipe 510; and a first steam supply valve 530 installed on the other side of the main steam supply pipe 510; And the main steam supply pipe 510 One end is connected to the doedoe, one end is connected between the main steam supply valve 520 and the first steam supply valve 530, the other end is connected to the main vent pipe 130, the ventilation inlet valve (120) and a first steam branch pipe 540 having the other end connected between the housing 210 of the sterilization filter unit 200; and a second steam supply valve 550 installed on the other side of the first branch steam pipe 540. And one end is connected to the first steam branch pipe 540, one end is connected to one side of the second steam supply valve 550, and the other end is connected to the ventilation branch pipe 160, the a second steam branch pipe 560 connected so that the other end is positioned between the first vent and discharge valve 170 and the second vent and discharge valve 180; and a third steam supply valve 570 installed in the second steam branch pipe 560 .

The test unit 400 includes a main supply pipe 420 having a cap 412 coupled thereto, a test vessel 410 filled with a medium therein, and the other end of the ventilation branch pipe 160 connected to one end; and the A first clamp 430 installed on one side of the main supply pipe 420 to open and close one side of the main supply pipe 420; and a first clamp 430 installed on the other side of the main supply pipe 420 to open and close the other side of the main supply pipe 420 One end is connected to the drain valve 440; and the main supply pipe 420, one end is connected to be positioned between the first clamp 430 and the drain valve 440, and the other end is connected to the test vessel 410 ); a sterilization air supply pipe 450 installed in the sterilization air supply pipe 450; an aeration valve 460 installed in the sterilization air supply pipe 450; and an exhaust pipe 470 having one end inserted into the test vessel 410 and installed; and a second clamp (480) installed on the other side of the exhaust pipe (470) to open and close the other side of the exhaust pipe (470).

In the process of independent sterilization of the sterilization filter using the sterilization filter unit 200, when the internal temperature of the incubator 300 reaches 60° C., the sterilization filter is installed inside the housing 210, and the sub vent discharge valve After confirming that 150 and the ventilation inlet valve 120 are closed, the main ventilation discharge valve 140 and the first ventilation discharge valve 170 are opened, and the lower discharge valve 222 and the second ventilation discharge valve 180 are opened. ), after opening 1/2 to 1/3, fully open the main steam supply valve 520, and slowly open the second steam supply valve 550, but at the same time opening 1/2, the pressure of the pressure gauge 240 It is characterized in that the sterilization process is performed for 15 minutes at a temperature of 121°C in a state maintained at 1.2Kg/cm2.

In the independent cooling and drying process using the sterilization filter unit 200, the second steam supply valve 550 is closed and the ventilation inlet valve 120 is opened at the same time, and the second ventilation outlet valve 180 and By controlling the upper discharge valve 232 and the lower discharge valve 222, it is confirmed through the pressure gauge 240 that the pressure inside the housing 210 is maintained at 1.5Kg/cm2 or more, and the cooling and drying process is performed for 20 minutes. characterized in that

In the process of testing the performance of the sterilization filter unit 200 using the test unit 400, before the test vessel 140 is mounted, the first vent/discharge valve 170 is closed while the second vent/discharge valve ( 180) and the third steam supply valve 570 are opened by 1/3 to confirm that the steam is discharged through the ventilation branch pipe 160, and then the second ventilation discharge valve 180 is closed, and then the main supply pipe 420 is One end is connected to the other end of the vent branch pipe 160 to mount the test container 410, and the second vent discharge valve 180 is slowly opened, 1/3 to 1/5 open, and then the first clamp ( 430) and the drain valve 440 are opened to sterilize the main supply pipe 420 for 15 minutes, then the third steam supply valve 570 is closed, and the first ventilation discharge valve 170 is opened for 10 minutes. (160) and the main supply pipe (420) are cooled and dried, the drain valve (440) is closed and the second clamp (480) is opened to secure an exhaust line, and then the aeration valve (460) is opened and then the second clamp (480) is opened. It is characterized in that the gas sterilized for 30 minutes is dissolved in the medium inside the test container 410 by controlling the opening of the single ventilation discharge valve 170 .

Independent sterilization of the sterilization filter and the performance test function of the sterilization filter according to the present invention presented as above is a microorganism incubator, that is, sterilization, cooling and drying processes are possible independently of the incubator. Not only does it minimize the performance degradation of the sterilization filter because it is not exposed, but also the sterilization, cooling and drying processes of the sterilization filter are possible at any time before sterilization of the incubator of microorganisms. The effect of significantly increasing the use time;

The performance of the sterilization filter can be tested at any time by attaching and detaching the test container to check the contamination of the air that has passed through the sterilization filter, so that the appropriate replacement time for the sterilization filter can be reliably determined. In addition to the inability to culture, it is possible to prevent the waste of expensive media, so it is possible to obtain the effect of preventing economic loss by reducing the waste of manpower and time as well as the cost of discarded media.

1 is a schematic diagram showing a conventional microbial incubator.
Figure 2 is a detailed schematic diagram showing a microorganism incubator according to a preferred embodiment of the present invention.
3 is a detailed schematic diagram showing a test unit according to a preferred embodiment of the present invention.
4 is a detailed schematic view showing steam movement in the sterilization filter sterilization process according to a preferred embodiment of the present invention.
5 is a detailed schematic view showing gas movement in the cooling and drying process of the sterilization filter according to the preferred embodiment of the present invention.
Figure 6 is a detailed schematic view showing the steam movement of the incubator sterilization process according to a preferred embodiment of the present invention.
7 is a detailed schematic view showing the gas movement of the cooling and drying process of the incubator according to a preferred embodiment of the present invention.
8 is a detailed schematic view showing steam movement in the test vessel mounting process, which is the first step, and the main supply pipe sterilization process, in the second step, according to a preferred embodiment of the present invention;
9 is a detailed schematic view showing gas movement in the cooling and drying process of the main supply pipe in the second step of confirming the performance of the sterilization filter according to the preferred embodiment of the present invention.
10 is a detailed schematic diagram showing the movement of gas in the process of supplying the sterilization gas into the test vessel, which is the third step of confirming the performance of the sterilization filter according to the preferred embodiment of the present invention.
11 is a detailed schematic diagram showing the test vessel installation and disassembly step, which is the fourth step of confirming the performance of the sterilization filter according to the preferred embodiment of the present invention.

The present invention relates to a microorganism incubator capable of sterilizing the sterilization filter of the sterilization filter unit 200 for sterilizing air supplied to the incubator 300 independently from the incubator 300 and testing the performance thereof, in more detail. In other words, the sterilization, cooling, and drying processes of the sterilization filter unit 200 can be performed at any time regardless of the sterilization, cooling and drying processes of the incubator 300 , so that the sterilization filter is used during the sterilization process of the incubator 300 . It is a technology related to a microorganism incubator equipped with independent sterilization of the sterilization filter and the performance test function of the sterilization filter that can smoothly achieve pure culture of microorganisms by significantly increasing the performance of the sterilization filter by minimizing the time exposed to high temperature.

In addition, in the present invention, the sterilization filter unit 200 can be sterilized, cooled, and dried at any time before sterilization of the incubator, so that the performance of the sterilization filter unit 200 can be extended, and the sterilization filter unit 200 can be sterilized at any time during the microbial culture period. In order to test the performance of 200 at any time, the test container 410 is attached and detached to prevent contamination of the culture medium due to deterioration of the performance of the sterilization filter unit 200, and at the same time, an appropriate replacement time of the sterilization filter unit 200 is performed. Since it can be accurately judged, it is impossible to pure culture of microorganisms due to contamination of the culture medium, and it is possible to prevent the discarding of expensive medium, thereby reducing the cost of discarded medium, as well as wasting of manpower and time, resulting in economic loss. It is a preventable technique.

Hereinafter, the present invention will be described in detail with reference to FIGS. 1 to 11 showing embodiments of the present invention.

First, referring to FIG. 2, referring to the configuration of a microorganism incubator (hereinafter referred to as a 'microbial incubator') having independent sterilization of the sterilization filter of the present invention and a performance test function of the sterilization filter, an air inlet for introducing air 100, a sterilization filter unit 200 for sterilizing and supplying air introduced from the air inlet unit 100, and receiving the sterilized air from the sterilization filter unit 200 to culture the microorganisms in pure water The incubator 300, which is branched and connected to the air inlet 100, is detachably connected, and the test unit 400 that receives the air that has passed through the sterilization filter unit 200 and the steam is introduced into the air. The sterilization filter unit 200 is supplied to the unit 100, but steam is supplied to one side of the air inlet unit 100 before passing through the sterilization filter unit 200 to sterilize the sterilization filter unit 200, or after passing through the sterilization filter unit 200 It is characterized in that it is configured to include a steam supply unit 500 for sterilizing the incubator 300 by supplying steam to the other side of the air inlet unit 100 .

Specifically, the air inlet 100 is to introduce air into the incubator, and an air inlet 110 for introducing air, a ventilation inlet valve 120 installed at the other end of the air inlet 110 and It is configured to include a main vent pipe 130 having one end installed on the vent inlet valve 120 .

The gas inlet pipe 110 introduces air, is finally connected to the incubator 300 through the main vent pipe 130, and measures the amount of gas introduced and a control valve capable of adjusting the amount of air introduced therein. It is composed of a gas flow meter.

At this time, the main vent pipe 130 is inserted and installed in the incubator 300 to be described in detail later, so that sterilization air or steam for sterilization can be supplied to the inside of the incubator 300 .

The ventilation inlet valve 120 is installed at the other end of the air inlet pipe 110 and is installed at one end of the main ventilation pipe 130 to communicate the air inlet pipe 110 and the main ventilation pipe 130, The other end of the air inlet pipe 110 and one end of the main vent pipe 130 are opened and closed.

In addition, when the ventilation inlet valve 120 is opened during the sterilization process of the sterilization filter unit 200 and the incubator 300 through the steam, the main vent pipe 130 has a reverse flow of steam to the air inlet pipe 110 . It may be configured to include a check valve 132 installed on one side to prevent it.

In addition, the air inlet 100 is installed on the other side of the main vent discharge valve 140 and the main vent pipe 130 installed on the other side of the main vent pipe 130, the other side of the main vent discharge valve 140 One end is connected to the other side of the sub vent discharge valve 150 and the main vent pipe 130 installed so as to be located in the , and one end is connected between the main vent discharge valve 140 and the sub vent discharge valve 150 . It is configured to include a vent branch pipe 160 and a first vent discharge valve 170 installed on the other side of the vent branch pipe 160 .

The main vent discharge valve 140 and the sub vent discharge valve 150 open and close the main vent pipe 130 so that the sterilization process of the sterilization filter unit 200 and the incubator 300 can be performed independently.

At this time, the main vent discharge valve 140 and the sub vent discharge valve 150 are selectively opened and closed to connect the vent branch pipe 160 connected to the test unit 400 to be described in detail later with the sterilization filter unit 200 and During the sterilization process of the incubator 300, it is to be sterilized.

The vent branch pipe 160 is branched from the main vent pipe 130, and is branched between the main vent exhaust valve 140 and the western vent exhaust valve 150, so that the sterilization filter unit 200 and the incubator 300 are separated. The sterilization air that is sterilized during the sterilization process and has passed through the sterilization filter unit 200 at any time during the culturing period of microorganisms is supplied to the test unit 400 to be described in detail later to test the performance of the sterilization filter 200 by the test unit ( 400) so that it can be tested.

That is, the vent branch pipe 160 is connected to the main supply pipe 420 of the test unit 400, which will be described in detail later, to selectively open and close the main vent discharge valve 140 and the sub vent discharge valve 150. It is sterilized before the incubation period of microorganisms.

The first ventilation discharge valve 170 is installed on the other side of the ventilation branch pipe 160 to open and close the ventilation branch pipe 160, so that the sterilization air that has passed through the sterilization filter 400 can be removed at any time during the culture period of microorganisms. It is selectively connected to the test unit 400 so that it can be supplied.

In addition, the air inlet 100 is installed on the other side of the ventilation branch pipe 160, the second ventilation discharge valve 180 is installed so as to be located on the other side of the first ventilation discharge valve 170, including The second vent discharge valve 180 is configured such that the other end of the second steam branch pipe 560 of the steam supply unit 500, which will be described in detail later, is connected between the first vent and discharge valve 180. , so that the main supply pipe 420 of the test unit 400 can be sterilized during the culturing period of the microorganisms.

In relation to the above, the sterilization filter unit 200 is installed in the main vent pipe 130 of the air inlet 100 , and the main vent pipe 130 through the air inlet 100 , that is, the air inlet pipe 110 . ), which sterilizes the air introduced into the air, so that the sterilized air is supplied to the incubator 300 so that the pure culture of microorganisms can be smoothly performed.

Specifically, the sterilization filter unit 200 includes a housing 210 installed in the main vent pipe 130 and having a sterilization filter installed therein, one end connected to a lower portion of the housing 210, and a lower discharge valve ( 222) is installed to control the pressure inside the housing 210, thereby controlling the flow rate of air during cooling together with the upper discharge valve 232, which will be described in detail later, or controlling the amount of steam inside the housing 210 during the sterilization process. A lower pipe 220 to control, one end is connected to the upper portion of the housing 210, and an upper discharge valve 232 is installed to adjust the pressure inside the housing 210, so that the lower discharge valve 222 The gas supplied into the housing 210 by being connected to the upper pipe 230 and the housing 210 to control the flow rate of air during cooling or to control the amount of steam inside the housing 210 during the sterilization process. Or it is configured to include a pressure gauge 240 for measuring the pressure of the steam.

At this time, the pressure gauge 240 may be installed on the upper pipe 230 , and may be installed to be located on one side of the upper discharge valve 232 to measure the pressure inside the housing 210 .

In the housing 210, when the air introduced from the air inlet 100 passes through the main vent pipe 130, air is introduced into the housing 210 so that the air is sterilized by the sterilization filter installed therein, which will be described in detail later. Steam is introduced from the steam supply unit 500 to be sterilized so that the sterilization filter is sterilized.

The lower pipe 220 and the upper pipe 230 are opened and closed through the lower discharge valve 222 and the upper discharge valve 232, respectively, by controlling the amount of air or steam flowing into the housing 210 together. , the pressure inside the housing 210 is regulated so that sterilization is controlled, and the gas can be smoothly sterilized and filtered by the sterilization filter installed inside the housing 210, for a detailed description of the use of the present invention. It should be done when explaining the embodiment.

The pressure gauge 240 allows to check the pressure inside the housing 210 when steam is introduced into the housing 210 from the steam supply unit 500, which will be described in detail later, to maintain an appropriate pressure for sterilization of the sterilization filter. and to check the pressure inside the housing 210 when air flows into the housing 210 from the air inlet 100 .

In connection with the above, the incubator 300 receives air from the main vent pipe 130 after the air introduced through the air inlet 100 is purified through the sterilization filter unit 200 to culture microorganisms. do.

That is, the incubator 300 is installed with the other side of the main vent pipe 130 of the air inlet 100 inserted and installed, as described above, to receive air from the main vent pipe 130 or to be described in detail later. The steam supplied from the steam supply unit 500 is sterilized by receiving the steam from the main vent pipe 130 .

At this time, the incubator 300 is configured to include a line 530 capable of supplying steam into the incubator in order to increase the temperature inside the incubator 300 .

In relation to the above, referring to FIG. 3 , the test unit 400 is branched and connected to the air inlet 100 , that is, connected to the other end of the ventilation branch pipe 160 , and a sterilization filter The air that has passed through the unit 200 is supplied to test the performance of the sterilization filter of the sterilization filter unit 200 .

Specifically, the test unit 400 has a cap 412 coupled to the upper portion, a test container 410 filled with an arrangement, and one end connected to the other end of the ventilation branch pipe 160 to sterilize air or steam. The main supply pipe 420 to move the first clamp 430 installed on one side of the main supply pipe 420 to open and close one side of the main supply pipe 420, and the other side of the main supply pipe 420 A drain valve 440 installed to open and close the other side of the main supply pipe 420 and one end connected to one end of the main supply pipe 420, one end between the first clamp 430 and the drain valve 440 The sterilization air supply pipe 450 is connected to be positioned and the other end is inserted into the test vessel 410 and installed, and the aeration valve 460 is installed in the sterilization air supply pipe 450 to open and close the sterilization air supply pipe 450 . ) and an exhaust pipe 470 having one side inserted into the test vessel 410 and installed on one side of the exhaust pipe 470 to open and close one side of the exhaust pipe 470 ) and exhaust sterilization It is characterized in that it is configured to include a filter (472).

The test container 410 is effective in terms of sterilization and deterioration of the test medium to use, but not necessarily limited thereto, to use a special glass made of pyrex, which is a special glass with excellent thermal shock resistance and chemical durability. ), as long as sterilization is possible and the test medium does not deteriorate during sterilization, it will be obvious that it is made of any material.

The cap 412 prevents contamination through the open upper part of the sterilized test container 410 , and the sterilization air supply pipe 450 and the exhaust pipe 470 maintain airtightness inside the test container 410 . and can be inserted and installed.

The main supply pipe 420 has one end connected to the other end of the ventilation branch pipe 160 , and is opened and closed through the aeration valve 460 , and is supplied through the ventilation branch pipe 160 . Allow the passed sterilization air to flow in or block.

In addition, the main supply pipe 160 , the main supply pipe 420 , the exhaust pipe 470 and related connection parts are made of a silicone material and can be easily opened and closed using a device such as tongs or clamps.

The first clamp 430 opens and closes one side of the main supply pipe 420 so that sterilization air or steam is moved or blocked through the main supply pipe 420 , so that the steam moves before installing the test vessel 410 . One side of the main supply pipe 420 is opened to test the performance of the sterilization filter unit 200 by introducing sterilization air into the test vessel 410 or by introducing the sterilization air into the test vessel 410, and the test vessel 410 is mounted and dismounted. In this case, the main supply pipe 420 is closed so that sterilization air or steam does not move.

The drain valve 440 is supplied to the main supply pipe 420 by a steam supply unit 500 to be described in detail later and is opened when the main supply pipe 420 is sterilized, and the sterilization air is supplied to the main supply pipe 420 . is introduced to supply sterilization air to the test vessel 410 , it is closed so that the sterilization air can be smoothly supplied to the test vessel 410 .

The sterilizing air supply pipe 450 is opened and closed through the aeration valve 460 to supply sterilizing air to the test container 410 so that the sterilizing air can be moved during testing as described above.

Unlike the first clamp 430 and the second clamp 480 to be described later, the aeration valve 460 is installed to more precisely control the movement of air through the sterilization air supply pipe 450 .

The exhaust pipe 470 allows the sterilization air introduced into the test container 410 to be discharged to the outside, and the other side is opened and closed through the second clamp 480 .

At this time, the exhaust pipe 470 is installed on the other side, and includes an exhaust sterilization filter 472 installed on one side of the second clamp 480 . The exhaust sterilization filter 472 is the test vessel 410 . ) before air is introduced into the inside of the test container 410 or when the pressure inside the test container 410 is lowered and external air flows into the inside of the test container 410 through the exhaust pipe 470 of the test container 410 Prevents interior contamination.

In connection with the above, the steam supply unit 500 supplies steam to the sterilization filter unit 200, the incubator 300, all connecting pipes through which the sterilization air moves, and the sterilization process of the sterilization filter unit 200 during the sterilization process. Steam is supplied to one side of the air inlet unit 100 before passing through the filter unit 200, that is, one side of the main vent pipe 130, or after passing through the sterilization filter unit 200 during the sterilization process of the incubator 300 Steam is supplied to the other side of the air inlet 100 , that is, the other side of the main vent pipe 130 .

Specifically, the steam supply unit 500 has the other end connected to the other side of the main vent pipe 130, and the other end is connected to the other side of the sub vent discharge valve 150 so as to supply steam. A main steam supply pipe 510 for sterilizing or sterilizing the air supplied to the incubator 300, and one side of the main steam supply pipe 510 installed on one side of the main steam supply pipe 510 By opening and closing, the main steam supply valve 520 for supplying or not supplying steam, and installed on the other side of the main steam supply pipe 510 to open and close the other side of the main steam supply pipe 510, the main vent pipe 130 ) is configured to include a first steam supply valve 530 to prevent the supply or supply of steam to the other side.

As described above, the main steam supply pipe 510 is a main pipe for supplying steam during the sterilization process of the incubator 300 , and is opened and closed by the main steam supply valve 520 .

In addition, the steam supply unit 500 has one end connected to the main steam supply pipe 510 , and one end is connected between the main steam supply valve 520 and the first steam supply valve 530 , and a main vent pipe between them. A first steam branch pipe 540 having the other end connected to 130 and the other end connected between the ventilation inlet valve 120 and the housing 210 of the sterilization filter unit 200, and the first steam branch pipe It is installed on the other side of the 540 is configured to include a second steam supply valve 550 for opening and closing the other side of the first steam branch pipe (540).

The first steam branch pipe 540 is connected to the main steam supply pipe 510 therebetween, and the other side is opened and closed by the second steam supply valve 550 to supply steam during the sterilization process of the sterilization filter unit 200 . enable or block

That is, the first steam branch pipe 540 is opened together with the closing of the main vent discharge valve 150 described above during the sterilization process of the sterilization filter unit 200 so that the sterilization filter unit 200 is sterilized independently. make it possible

In addition, the steam supply unit 500 is connected so that one end is connected to the first steam branch pipe 540, one end is located at one side of the second steam supply valve 550, and the ventilation branch pipe 160. The other end is connected to the second steam branch pipe 560 and the second steam branch pipe 560, which are connected so that the other end is positioned between the first ventilation and discharge valve 170 and the second ventilation and discharge valve 180. ) and is configured to include a third steam supply valve 570 for opening and closing the second steam branch pipe 560 .

When the second steam branch pipe 560 is opened and closed by the third steam supply valve 570 to sterilize the main supply pipe 420 of the test unit 400 described above, the steam passes through the main vent pipe 130 . By allowing direct supply to the main supply pipe 420 without passing through, the main supply pipe 420 can be sterilized before the test container 410 is mounted when the performance of the sterilization filter unit 200 is checked.

In addition, in order to purely culture microorganisms, all lines that come into contact with the culture medium, that is, pipes, must be sterilized, cooled, and dried. In particular, in order to cultivate aerobic microorganisms, the sterilization filter is also sterilized, cooled and dried in the same way. Thus, air that is not contaminated with various germs should be supplied to the incubator 300 .

At this time, the sterilization filter used in the sterilization filter unit 200 of the present invention uses Germany's Parker/dominick hunter, 0.2㎛, High Flow Tertpor Ⅱ made of special synthetic resin, and when exposed to high temperature for a long time, the sterilization function is significantly reduced, The manufacturer provides performance information that the average lifespan of these sterilization filters can be used about 30 to 40 times based on sterilization at 121°C and 1.2Kg/cm2 for 13 to 16 minutes.

That is, the conventional microbial incubator supplies steam to the jacket of the incubator and indirectly heats it without a separate sterilization, cooling, and drying process for the sterilization filter, so that when the internal temperature of the vessel reaches 90~98℃, the lower discharge line and sampling of the vessel When the internal temperature of the vessel reaches 121℃, 1.2Kg/cm2 by supplying steam to the ventilation line through the sterilization filter at the same time as supplying steam to the line, sterilization is performed in the form of maintaining for 13~16 minutes and then the steam supply is stopped It is common to perform pure culture by sterilizing and cooling the vessel and the aeration line in a manner that supplies cooling water to the jacket and vents air through the aeration line at the same time, and then inoculates the seed culture.

In this case, during one-time sterilization with a jacket, the sterilization filter is exposed to excessively high heat of steam for about 45 to 60 minutes, and the sterilization performance is remarkably deteriorated. Because it is ventilated, it acts as a great limiting factor for the pure culture of microorganisms, and since the performance of the sterilization filter cannot be verified during the incubation period, the culture medium is suddenly contaminated and the culture medium, that is, the medium must be discarded. occurred.

In order to solve this problem, the present invention makes it possible to independently sterilize, cool, and dry the sterilization filter unit 200 at any time through the above-described configurations.

That is, the microorganism incubator of the present invention having the above-described configurations can reduce the exposure time to high temperature of steam to 15 minutes when the sterilization filter unit 200 is sterilized, thereby maintaining the maximum performance time, In the sterilization filter, if moisture remains, there is a high possibility that contaminants may be mixed in the water droplets and introduced into the incubator 300, which causes the sterilization efficiency to decrease. Therefore, cooling and drying processes must be performed independently. Since the sterilization, cooling, and drying processes of the sterilization filter unit 200 are possible, it is possible to prevent the cause of the decrease in sterilization efficiency as described above, thereby culturing aerobic microorganisms without problems.

In addition, since the microorganism incubator of the present invention can independently sterilize, cool and dry the incubator 300, it is possible to increase the temperature inside the incubator 300 to 121° C. through steam, so that the aeration line and the incubator 300 Sterilization can be performed more smoothly.

In addition, the test unit 400 used in the present invention is sterilized after filling the inside of the test container 410 with a liquid medium (NB: Nutrient Broth) and sterilizing it in an autoclave, cooling it on a clean bench (sterile work bench) The gas sterilized by the filter unit 200 is supplied to the inside of the test container 410 for about 30 minutes, and the test container 410 is moved to a clean bench again, and the medium supplied with the sterilized air in a sterile state is used as a test solid. After inoculating about 0.1 ml of medium (NA: Nutrienr Agar) in an incubator (incubator: microbial stationary incubator) for 16 hours at a temperature of 35 ° C. When colonies are formed in the solid medium, the sterilization filter unit 200 is By judging the performance of the sterilization filter unit 200 in such a way that it is determined that the sterilization filter unit 200 has reached the end of the life period of the sterilization filter unit 200, it is possible to confirm the appropriate replacement time of the sterilization filter unit 200, and vice versa. If is not generated, it is determined that the sterilization filter unit 200 is not contaminated, and it is determined that the life time of the sterilization filter unit 200 remains.

Hereinafter, the air inlet unit 100, the sterilization filter unit 200, the incubator 300, the test unit 400, and the steam supply unit 500 described above will be described while explaining the example of use of the microorganism incubator of the present invention. and the opening and closing of the valve will be described together.

First, since the sterilization process of the sterilization filter must be performed before the internal temperature of the incubator 300, which is the time when steam is supplied to the inside of the incubator 300, reaches 85 to 98° C., the execution time of the sterilization process of the sterilization filter is determined by the operator. It can be carried out according to the convenience of the incubator, but since it usually takes about 30 minutes, it must be started before the temperature inside the incubator 300 reaches 65 ° C. Before supplying steam to the inside of the incubator 300, it is quickly and efficiently sterilized. Sterilization and cooling processes of the filter unit 200 may be performed.

That is, the sterilization filter sterilization process starts with installing the sterilization filter inside the housing 210 when the internal temperature of the incubator 300 reaches 60° C., and all valves are closed.

The sterilization filter sterilization process is a process of independently sterilizing the sterilization filter unit 200 by supplying steam from the steam supply unit 500 .

4, after confirming that the sub vent discharge valve 150 and the vent inlet valve 120 are closed, the main vent discharge valve 140 and the first vent discharge valve 170 are opened, and the lower The discharge valve 222 and the second vent discharge valve 180 are opened by 1/2 to 1/3 to secure a state in which steam can be discharged to the outside.

4, the main steam supply valve 520 is completely opened to supply steam to the main steam supply pipe 540, and the second steam supply valve 550 is gradually opened, but 1/2 opened. Steam is supplied to one side of the main vent pipe 130 , the upper discharge valve 232 is gradually opened, and the pressure of the pressure gauge 240 is checked.

Thereafter, by performing the sterilization process of the sterilization filter unit 200 for 15 minutes while the pressure of the pressure gauge 240 is maintained at 1.2Kg/cm 2 , the sterilization filter inside the housing 210 is sterilized.

That is, in the sterilization filter sterilization process, one side of the main vent pipe 130, that is, the one side where the sub vent discharge valve 150 is installed, and the sterilization filter unit 200 and the vent branch pipe 160 are sterilized through the control of the valve as described above. make it possible

Next, in the cooling and drying process of the sterilization filter, the steam supplied from the steam supply unit 500 is cut off, and air is introduced from the air inlet unit 100 to independently cool and dry the sterilization filter unit 200 .

As shown in FIG. 5 , the second steam supply valve 550 opened in the sterilization filter sterilization process described above is closed to block the steam supplied to the main vent pipe 130 and at the same time, the vent inlet valve 120 . is opened so that air is supplied to the main vent pipe 130 through the air inlet pipe 110 .

At this time, the air supplied to the air inlet pipe 110 moves quickly because compressed air is supplied through the compressor, so that the steam remaining in the main vent pipe 130 can be efficiently dried, and dried at the same time as the cooling process is performed. The process proceeds together.

Thereafter, as shown in FIG. 5, the pressure inside the housing 210 is maintained at 1.5Kg/cm2 or more by adjusting the second vent discharge valve 180, the upper discharge valve 232, and the lower discharge valve 222. Check it through the pressure gauge 240 and allow the sterilization filter unit 200 to cool and dry for 20 minutes.

Next, in the incubator sterilization process, steam is supplied from the steam supply unit 500 to the incubator 300 to sterilize independently of the sterilization filter unit 200 .

In this incubator sterilization process, it is preferable to first confirm that the temperature inside the incubator 300 reaches 95 to 98° C. after the sterilization filter sterilization process and the cooling and drying process are completed, and to supply steam from the steam supply unit 500 . However, this is because if the temperature inside the incubator 300 does not reach the above range, a knocking phenomenon may occur.

That is, the incubator sterilization process confirms that the temperature inside the incubator 300 has reached 95 ~ 98 ℃, closes the previously opened main vent discharge valve 140, as shown in FIG. 1 By opening the steam supply valve 530, the other side of the main vent pipe 130, that is, the sub vent discharge valve 150 is opened to supply steam to the other side, and at the same time to supply steam into the incubator 300.

At this time, since the main steam supply valve 520 is in a previously opened state, and the second steam supply valve 550 is in a closed state, when the first steam supply valve 530 is opened, the steam flows through the main vent pipe 130 . As soon as it is supplied to the other side, it is moved into the incubator 300 .

Then, as shown in FIG. 6 , the sub vent discharge valve 150 is opened to open the other side of the main vent pipe 130 , that is, from the other end of the main vent pipe 130 to the sub vent discharge valve 150 by steam. Allow sterilization to take place.

At this time, since the main vent discharge valve 140 has been previously closed, steam does not flow back into the sterilization filter unit 200, and the sterilization filter unit 200 maintains a dry state.

Thereafter, the sterilization process of the incubator 300 is performed for 15 minutes while the pressure inside the incubator 300 is 1.2Kg/cm 2 and the temperature is maintained at 121°C.

Next, in the incubator cooling and drying process, after the incubator sterilization process is completed, sterilizing air is supplied to the inside of the incubator 300 through the air inlet 100 and the sterilized and dried sterilizing filter 200 to be cooled.

That is, in the incubator cooling process, as shown in FIG. 7 , after the incubator sterilization process is completed, the first aeration exhaust valve 170 and the second aeration exhaust valve 180 that were previously opened are closed, and then the main steam is supplied. The valve 520 is closed to cut off the steam supply, and the upper discharge valve 232 and the lower discharge valve 222 are blocked so that the supplied sterilizing air can be smoothly supplied to the incubator 300 .

Thereafter, as shown in FIG. 7, when the main vent discharge valve 140 is opened, the sterilization filter unit 200 is sterilized, and sterilization air is supplied to the inside of the incubator 300 to cool the incubator 300, If the temperature inside the incubator 300 is adjusted to an appropriate temperature, that is, an appropriate temperature for culturing microorganisms, and then inoculating the seed, microorganisms, that is, aerobic microorganisms can be purely cultured, and at this time, the culture process such as the temperature of the incubator 300 Variables are under automatic control.

Through the above process, before culturing microorganisms, the sterilization filter unit 200 and the incubator 300 can be sterilized independently, thereby obtaining the effect of increasing the service life of the sterilization filter unit 200 compared to the prior art. can

In addition, in this process, it will be obvious that manual and automatic control is already possible in a way that low-action control by PLC (Programmable Logic Control) is possible, and since the opening sequence of the valves described above is a sequence to minimize contamination, the same sequence as above It is preferable to open and close with

When the sterilization filter sterilization, cooling and drying process as described above and the incubator sterilization and cooling process are completed, the microorganism is cultured and the test unit 400 is installed to check the performance of the sterilization filter unit 200 during the microbial culture period. It can be attached to and detached from the ventilation branch pipe 160 described above, and the process of verifying the performance of the sterilization filter unit 200 through the test unit 400 will be described below.

First, as a solid medium preparation step, which is the first step of confirming the performance of the sterilization filter using the test unit 400, the solid medium is nutrient broth (a nutrient medium for culturing bacteria as Nutrient Broth) at 8.0 g/L After dissolving the medium composed of 2.0% agar and autoclaving for 15 minutes at a temperature of 121°C and a pressure of 1.2Kg/cm2, cooled to 45°C and sterilized by aseptic operation on a clean bench. After solidifying by aliquoting 15 to 20 ml in a Petri dish (Petri-Dish: solid medium plate), incubate at 35° C. for 16 hours or more to select and use only plates that do not form colonies. This process is preferably prepared at least one day before the performance of the sterilization filter unit 200 is tested.

The next step is to prepare a test container, and the solid medium is tested by injecting 100 to 300 ml of a medium composed of a nutrient broth concentration of 8.0 g/L into the test container 410 and loosely coupling the cap 412. To allow the air inside the container 410 to be discharged to the outside, and to prevent foreign substances from penetrating into the inside of the test container 410, the outside is wrapped with a foil or the like.

After confirming that the aeration valve 460 is completely closed, the drain valve 440 is 1/2 opened, and the main supply pipe 420 is after the first clamp 430 is loosely fixed, that is, the main supply pipe 420 is closed. After making the one side and the other side open, the end of the main supply pipe 420 is wrapped with a foil or the like to prevent foreign substances from penetrating.

At this time, the second clamp 480 installed on one side of the exhaust sterilization filter 472 is fixed to 1/3 so that when the gas of the medium is discharged or the pressure inside the test vessel 410 is lowered, the outside air is introduced in a sterilized state. The other side of the sterilization air supply pipe 450 is installed to be completely submerged in the medium, and one end of the exhaust pipe 470 is installed to be sufficiently spaced from the medium at a temperature of 121°C and a pressure of 1.2Kg/cm2. Prepare by autoclaving for 15 minutes and cooling to room temperature in Clean Benche.

Then, as shown in FIG. 8, in the state in which the first vent and discharge valve 170 is closed before mounting the prepared test vessel 410, the second vent and discharge valve 180 and the third steam supply valve 570. After confirming that steam is discharged through the ventilation branch pipe 160 by opening 1/3 of the It is connected to the other end of the branch pipe 160 to mount the test vessel (410).

Next, the main supply pipe sterilization, cooling and drying process, which is the second step of confirming the performance of the sterilization filter, blocks the supplied steam and introduces the gas supplied to the incubator 300 into the main supply pipe 420 to the main supply pipe 420 . is cooled and dried.

That is, as shown in FIG. 8, in the main supply pipe sterilization, cooling and drying process, after the test vessel 410 is mounted in the test vessel mounting process, the second vent discharge valve 180 is slowly opened, but 1/ After opening 4 to 1/5, the first clamp 430 and the drain valve 440 are opened so that the main supply pipe 420 can be sterilized for 15 minutes.

Thereafter, as shown in FIG. 9, the third steam supply valve 570 is closed to cut off the steam supply, and the first ventilation discharge valve 170 is opened for 10 minutes to the ventilation branch pipe 160 and the main supply pipe 420. ) is cooled and dried.

Next, in the third step of confirming the performance of the sterilization filter, the sterilization gas supply process into the test container introduces the sterilized and purified gas through the sterilization filter unit 200 into the test container 410 .

That is, in the process of supplying the sterilization gas to the inside of the test container, as shown in FIG. 10 , after the cooling and drying process of the main supply pipe, the drain valve 440 is closed and the second clamp 480 is opened at the same time to open the exhaust line. After ensuring that the gas is supplied to the test container 410 when the sterilization air supply pipe 450 is opened, external air is introduced into the test container 410 or the air inside the test container 410 is discharged to the outside. To make it possible, the internal pressure of the test container 410 is made to be stable.

Thereafter, as shown in FIG. 10 , the aeration valve 460 of the sterilization air supply pipe 450 is opened so that gas can be supplied into the test vessel 410 , and then the opening of the first vent discharge valve 170 is performed. Controlled so that the gas is supplied to the inside of the test container 410, and aerated for 30 minutes so that the sterilized gas is dissolved in the medium inside the test container 410.

Next, in the test container mounting and dismounting step, which is the fourth step of confirming the performance of the sterilization filter, the gas supplied to the inside of the test container 410 is blocked and the ventilation branch pipe 160 and the main supply pipe 420 are separated so that aseptic operation is possible. Transfer to a clean bench.

That is, in the test vessel dismounting step, as shown in FIG. 11 , after closing the second vent/discharge valve 180 , the first vent/discharge valve 170 is blocked to cut off the supply of gas, and the second After blocking the clamp 480 and the first clamp 430 , the ventilation branch pipe 160 and the main supply pipe 420 are separated, and then the test unit 400 is transferred to the clean bench.

After aseptic operation in a clean bench, 1.0 ml of aerated medium is inoculated into solid medium with a sterilized micropipette, well smeared, and cultured at 35° C. for at least 16 hours in a prepared stationary incubator to observe colony formation.

If colonies are formed, it is determined that the life period of the sterilization filter unit 200 has arrived, and after replacing the sterilization filter unit 200, sterilization, cooling, and drying processes are independently performed. If no colonies are formed, sterilization It is determined that the life period of the filter unit 200 still remains, so that it can be continuously used.

As a result, the microorganism incubator of the present invention can sterilize and cool the sterilization filter unit 200 and the incubator 300 independently, so that not only can the service life of the sterilization filter unit 200 be increased compared to the prior art, but also microorganisms of the sterilization filter unit 200 by testing the performance of the sterilization filter unit 200 at any time during the culturing period of microorganisms so that the sterilization filter unit 200 can be replaced at an appropriate time. By preventing microbial contamination by (200), it is possible to obtain the effect of smoothly culturing pure microorganisms.

The above has been described with reference to a preferred embodiment of the present invention, and is not limited to the above embodiment, and a person skilled in the art through the above embodiment does not deviate from the gist of the present invention It can be implemented with various changes in

100: air inlet
110: air inlet pipe 120: ventilation inlet valve
130: main vent pipe 140: main vent discharge valve
150 ; Sub vent discharge valve 160: vent branch pipe
170: first vent exhaust valve 180: second vent exhaust valve
200: sterilization filter unit
210: housing 220: lower pipe
222: lower discharge valve 230: upper pipe
232: upper discharge valve 240: pressure gauge
300: incubator
400: test unit
410: test vessel 420: main supply pipe
430: first clamp 440: drain valve
450: sterilization air supply pipe 460: aeration valve
470: exhaust pipe 480: second clamp
500: steam supply unit
510: main steam supply pipe 520: main steam supply valve
530: first steam supply valve 540: first steam branch pipe
550: second steam supply valve 560: second steam branch pipe
570: third steam supply valve

Claims (9)

Air inlet 100 for introducing air; and
A sterilization filter unit 200 for sterilizing the air introduced from the air inlet unit 100; and
an incubator (300) receiving the sterilized air from the sterilization filter unit (200); Wow
a test unit 400 branched and connected to the air inlet unit 100 and receiving the sterilization air that has passed through the sterilization filter unit 200 to perform a performance test; and
The steam is supplied to the air inlet 100 , but is supplied to one side of the air inlet 100 before passing through the sterilization filter 200 or to the other side of the air inlet 100 after passing through the sterilization filter 200 . Includes; steam supply unit 500 for supplying;
The steam supply unit 500 is
The other end is connected to the other side of the main vent pipe 130, the main steam supply pipe 510 is connected so that the other end is located on the other side of the sub vent discharge valve 150 to supply steam; and
A main steam supply valve 520 installed on one side of the main steam supply pipe 510; and
A first steam supply valve 530 installed on the other side of the main steam supply pipe 510; And
One end is connected to the main steam supply pipe 510, one end is connected between the main steam supply valve 520 and the first steam supply valve 530, and the other end is connected to the main vent pipe 130, A first steam branch pipe 540 having the other end connected between the ventilation inlet valve 120 and the housing 210 of the sterilization filter unit 200; and
A second steam supply valve 550 installed on the other side of the first steam branch pipe 540; and
One end is connected to the first steam branch pipe 540, one end is connected to one side of the second steam supply valve 550, and the other end is connected to the ventilation branch pipe 160, the first ventilation is discharged. a second steam branch pipe 560 connected so that the other end is positioned between the valve 170 and the second vent discharge valve 180; and
A microorganism incubator having a function of independent sterilization of a sterilization filter and a performance test function of the sterilization filter, characterized in that it comprises; a third steam supply valve (570) installed in the second steam branch pipe (560).
The method of claim 1,
The air inlet 100 is
Air inlet pipe 110 for introducing air; and
a ventilation inlet valve 120 installed at the other end of the air inlet pipe 110; and
A microorganism incubator having a function of independent sterilization of a sterilization filter and a performance test function of the sterilization filter, characterized in that it comprises; a main vent pipe 130 having one end installed on the ventilation inlet valve 120 .
The method of claim 1,
The sterilization filter unit 200 sterilizes the incoming air,
A housing 210 installed in the main vent pipe 130 and having a built-in sterilization filter installed therein; and
A lower pipe 220 having one end connected to the lower part of the housing 210 and having a lower discharge valve 222 installed; and
an upper pipe 230 having one end connected to the upper portion of the housing 210 and having an upper discharge valve 232 installed; and
Independent sterilization of a sterilization filter characterized in that it includes; a pressure gauge 240 that is connected to the housing 210 and measures the pressure inside the housing 210 and a microorganism having a function of testing the performance of the sterilization filter incubator.
The method of claim 1,
The air inlet 100 is
The main vent discharge valve 140 installed on the other side of the main vent pipe 130; and
Doedoe installed on the other side of the main vent pipe 130, the sub vent discharge valve 150 is installed so as to be located on the other side of the main vent discharge valve 140; And
a vent branch pipe 160 having one end connected to the other side of the main vent pipe 130 and having one end connected between the main vent discharge valve 140 and the sub vent discharge valve 150; and
Independent sterilization of the sterilization filter, characterized in that it includes; a second ventilation and discharge valve 180 installed on the other side of the ventilation branch pipe 160 and installed to be located on the other side of the first ventilation and discharge valve 170 and a microorganism incubator equipped with a performance test function of the sterilization filter.
delete The method of claim 1,
The test unit 400
A cap 412 is coupled to the upper portion, and a test container 410 filled with a medium therein and
The main supply pipe 420 having one end connected to the other end of the ventilation branch pipe 160; and
A first clamp 430 installed on one side of the main supply pipe 420 to open and close one side of the main supply pipe 420; and
A drain valve 440 installed on the other side of the main supply pipe 420 to open and close the other side of the main supply pipe 420; and
One end is connected to the main supply pipe 420 , and one end is connected between the first clamp 430 and the drain valve 440 to be positioned, and the other end is inserted and installed in the test container 410 . supply pipe 450; and
an aeration valve 460 installed in the sterilization air supply pipe 450; and
an exhaust pipe 470 having one side inserted into the test vessel 410 and installed; and
A second clamp (480) installed on the other side of the exhaust pipe (470) to open and close the other side of the exhaust pipe (470). A microbial incubator.
The method of claim 1,
Independent sterilization of the sterilization filter using the sterilization filter unit 200 includes:
When the internal temperature of the incubator 300 reaches 60° C., a sterilization filter is installed inside the housing 210, and it is confirmed that the sub-vent discharge valve 150 and the ventilation inlet valve 120 are closed, and then the main ventilation After opening the discharge valve 140 and the first ventilation discharge valve 170, the lower discharge valve 222 and the second ventilation discharge valve 180 are opened 1/2 to 1/3, and then the main steam supply valve 520 ) is fully opened, and the second steam supply valve 550 is slowly opened, but at a temperature of 121° C. for 15 minutes while opening 1/2 and maintaining the pressure of the pressure gauge 240 at 1.2 Kg/cm 2 Independent sterilization of the sterilization filter, characterized in that the sterilization process is carried out, and a microorganism incubator equipped with a performance test function of the sterilization filter.
The method of claim 1,
The independent cooling and drying process using the sterilization filter unit 200 is,
The second steam supply valve 550 is closed and the vent inlet valve 120 is opened at the same time, and the second vent exhaust valve 180, the upper exhaust valve 232, and the lower exhaust valve 222 are adjusted to adjust the housing ( 210) Independent sterilization of the sterilization filter, characterized in that the cooling and drying process is performed for 20 minutes while checking that the internal pressure is maintained at 1.5Kg/cm 2 or more through the pressure gauge 240. Equipped with a function of testing the performance of the sterilization filter A microbial incubator.
The method of claim 1,
The process of testing the performance of the sterilization filter unit 200 using the test unit 400 is,
Before mounting the test vessel 140, in the state in which the first ventilation and discharge valve 170 is closed, the second ventilation and discharge valve 180 and the third steam supply valve 570 are opened by 1/3 to open the ventilation branch pipe 160 After confirming that the steam is discharged through the ), after closing the second ventilation discharge valve 180, one end of the main supply pipe 420 is connected to the other end of the ventilation branch pipe 160, and the test vessel 410 is mounted and ,
Slowly open the second vent discharge valve 180, 1/3 to 1/5, and then open the first clamp 430 and drain valve 440 to sterilize the main supply pipe 420 for 15 minutes. 3 Close the steam supply valve 570, open the first ventilation discharge valve 170, and cool and dry the ventilation branch pipe 160 and the main supply pipe 420 for 10 minutes,
After closing the drain valve 440 and opening the second clamp 480 at the same time to secure the exhaust line, the aeration valve 460 is opened, and then the opening of the first ventilation and discharge valve 170 is adjusted to eliminate the bacteria for 30 minutes. A microorganism incubator with independent sterilization of the sterilization filter and the performance test function of the sterilization filter, characterized in that the gas is dissolved in the medium inside the test vessel (410).

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