CN211947072U - Plant cell culture tank system - Google Patents
Plant cell culture tank system Download PDFInfo
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- CN211947072U CN211947072U CN201922453171.0U CN201922453171U CN211947072U CN 211947072 U CN211947072 U CN 211947072U CN 201922453171 U CN201922453171 U CN 201922453171U CN 211947072 U CN211947072 U CN 211947072U
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Abstract
The utility model provides a plant cell culture tank system, which comprises a culture tank, an air main pipeline, a fine steam main pipeline and a coarse steam main pipeline; the bottom of the culture tank is provided with an air inlet pipe, a main air inlet pipeline is communicated with the main air pipeline through an air inlet pipeline, and the main air inlet pipeline is communicated with the main fine steam pipeline through a fine steam inlet pipeline; the side wall of the culture tank is provided with an interlayer, and the side surface of the culture tank is provided with an interlayer water inlet pipe and an interlayer water outlet pipe; the bottom of the culture tank is also provided with a discharge pipe which is communicated with the crude steam main pipeline through a discharge sterilization pipe; a sampling port is arranged on the side surface of the culture tank, and a sampling port sterilizing pipe communicated with the crude steam main pipeline is arranged on the sampling port; the main air inlet pipeline is also communicated with the crude steam main pipeline through a crude steam inlet pipeline, and a small exhaust pipe is arranged at the communication position of the crude steam inlet pipeline and the main air inlet pipeline. The utility model discloses can provide stable temperature, pressure and air volume for plant cell culture to guarantee that the culture environment is free from miscellaneous fungus, help improving the output of plant cell culture engineering.
Description
Technical Field
The utility model relates to a biotechnology engineering equipment field, concretely relates to plant cell culture jar system.
Background
In biotechnology, plant cell culture, also called in vitro culture in a broad sense, refers to a technique of separating desired tissues, organs or cells, protoplasts, etc. from a plant body, and inoculating the isolated tissues, organs or cells, protoplasts, etc. to a culture medium containing various nutrients and plant hormones under aseptic conditions for culture to obtain regenerated whole plants or to produce other products having economic value. In plant cell culture, it is necessary to provide appropriate temperature, pressure and aeration conditions for plant cell tissues and to ensure that the culture is performed in a sterile environment.
The existing plant cell culture system has the defects that stable temperature, pressure and ventilation conditions are difficult to provide, and the non-infectious bacteria is difficult to strictly ensure, so that the yield of plant cell culture engineering is influenced.
Therefore, it is necessary to provide a new plant cell culture system that provides a suitable environment for plant cell culture.
SUMMERY OF THE UTILITY MODEL
For solving some plant cell culture systems and being difficult to provide stable temperature, pressure and the condition of ventilating to be difficult to strictly guarantee aseptic problem, the utility model provides a plant cell culture jar system can provide stable temperature, pressure and air volume for plant cell culture, and guarantees that the culture environment does not have miscellaneous fungus, helps improving the output of plant cell culture engineering.
The utility model discloses the technical scheme who adopts specifically is:
the plant cell culture tank system comprises a plurality of culture tanks, an air main pipeline, a fine steam main pipeline and a coarse steam main pipeline; the bottom of the culture tank is provided with an air inlet pipe communicated with a main air inlet pipeline, the air inlet pipe is provided with a first switch valve and a first regulating valve, the main air inlet pipeline is communicated with the main air pipeline through an air inlet pipeline, the air inlet pipeline is provided with a second switch valve, the main air inlet pipeline is provided with a third switch valve, the main air inlet pipeline is communicated with the main fine steam pipeline through a fine steam inlet pipeline, and the fine steam inlet pipeline is provided with a fourth switch valve; the bottom of the culture tank is also provided with a discharge pipe, the discharge pipe is communicated with a crude steam main pipeline through a discharge sterilizing pipe, the discharge sterilizing pipe is provided with a fifth switch valve, the discharge pipe is respectively provided with a sixth switch valve and a seventh switch valve above and below the communicated part with the discharge sterilizing pipe, and the tank body of the culture tank is provided with a barometer.
The utility model provides an among the plant cell culture tank system, culture tank side is equipped with the sample connection, and the sample connection is equipped with the sample connection sterilization pipe that communicates with thick steam trunk line, is equipped with the eighth ooff valve on the sample connection sterilization pipe.
The utility model provides an among the plant cell culture jar system, main air inlet pipeline still communicates with thick steam trunk line through thick steam air inlet pipeline, and thick steam air inlet pipeline is equipped with the ninth ooff valve, and thick steam air inlet pipeline is equipped with little blast pipe with main air inlet pipeline's intercommunication department, and little blast pipe is equipped with the tenth ooff valve.
The utility model provides an among the plant cell culture jar system, main air inlet pipeline is equipped with the filter.
The utility model provides an among the plant cell culture jar system, the air inlet pipeline still is equipped with flowmeter and eleventh ooff valve, and the flowmeter is installed between second ooff valve and eleventh ooff valve.
The utility model provides an among the plant cell culture tank system, the culture tank top is equipped with the tail gas pipe, and the tail gas pipe is equipped with the twelfth ooff valve.
In the plant cell culture tank system provided by the utility model, the system also comprises a cold water inlet main pipeline, a cold water return main pipeline, a low-temperature water inlet main pipeline, a low-temperature water return main pipeline, a hot water inlet main pipeline and a hot water return main pipeline; the side wall of the culture tank is provided with an interlayer, and the side surface of the culture tank is provided with an interlayer water inlet pipe and an interlayer water outlet pipe; the interlayer water inlet pipe is communicated with a hot water inlet main pipeline through a hot water inlet guide pipe, the hot water inlet guide pipe is provided with a second regulating valve and a thirteenth switch valve, the interlayer water inlet pipe is communicated with a low-temperature water inlet main pipeline through a low-temperature water inlet guide pipe, the low-temperature water inlet guide pipe is provided with a third regulating valve, a fourteenth switch valve and a fifteenth switch valve, the low-temperature water inlet guide pipe is communicated with a cold water inlet main pipeline through a cold water inlet guide pipe, the communication position of the cold water inlet guide pipe and the low-temperature water inlet guide pipe is positioned between the fourteenth switch valve and the fifteenth switch valve, and the cold water inlet guide pipe is provided with a sixt; the interlayer water outlet pipe is communicated with the hot water return main pipeline through a hot water return pipe, the hot water return pipe is provided with a fourth regulating valve and a seventeenth switch valve, the interlayer water outlet pipe is communicated with the low-temperature water return main pipeline through a low-temperature water return pipe, the low-temperature water return pipe is provided with a fifth regulating valve, an eighteenth switch valve and a nineteenth switch valve, the low-temperature water return pipe is communicated with the cold water return main pipeline through a cold water return pipe, the communication position of the cold water return pipe and the low-temperature water return pipe is positioned between the eighteenth switch valve and the nineteenth switch valve, and the cold water return pipe is provided with a twentieth switch.
The utility model provides an among the plant cell culture jar system, the intermediate layer outlet pipe is equipped with the intermediate layer sterilizing tube with the initial steam trunk line intercommunication, and the intermediate layer sterilizing tube is equipped with twenty-first ooff valve.
The utility model provides an among the plant cell culture jar system, the culture tank includes dona tub and cell culture jar, and the quantity of dona tub is one, and the quantity of cell culture jar is two and above.
The utility model provides a beneficial effect that technical scheme brought is:
(1) pure air is introduced into the culture tank through the main air pipeline, the air flow introduced into the culture tank can be regulated and controlled, appropriate ventilation quantity can be provided for plant cell culture, and the pressure in the tank is measured and displayed through a barometer on the tank body.
(2) The culture tank is provided with an interlayer which is communicated with the cold water inlet main pipeline, the cold water return main pipeline, the low-temperature water inlet main pipeline, the low-temperature water return main pipeline, the hot water inlet main pipeline and the hot water return main pipeline in an adjustable and controllable manner, the temperature of the tank body can be flexibly controlled, and the proper environment temperature can be provided for plant cell culture.
(3) Can carry out high temperature sterilization to the discharging pipe of cultivateing the jar, sample connection, these positions with external environment contact of air inlet pipeline and intermediate layer through thick steam trunk line for cultivateing jar lets in thick steam, and the setting of little blast pipe can carry out thorough high temperature steam sterilization to main air inlet pipeline on the main air inlet pipeline, and dead angle when avoiding the sterilization can provide no miscellaneous fungus environment for plant cell culture.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic structural view of an embodiment of a plant cell culture tank system provided by the present invention;
FIG. 2 is a schematic view showing the structure and connection relationship of a culture tank according to an embodiment of the present invention;
FIG. 3 is a schematic view showing the structure and connection of a cell culture tank according to an embodiment of the present invention.
Detailed Description
For solving some plant cell culture systems and being difficult to provide stable temperature, -pressure and the condition of ventilating to be difficult to strictly guarantee aseptic problem, the utility model provides a plant cell culture jar system embodiment can provide stable temperature, pressure and air volume for plant cell culture, and guarantees that the culture environment does not have miscellaneous fungus, helps improving the output of plant cell culture engineering.
In order to make the objects, technical solutions and advantages of the present invention clearer, embodiments of the present invention will be described in further detail below with reference to the accompanying drawings.
Examples
The utility model provides a plant cell culture tank system embodiment, overall structure is as attached figure 1, including a plurality of culture tank, air trunk line, smart steam trunk line and thick steam trunk line. Wherein, the main air pipeline is connected with an air filtering system, and pure air which is filtered and sterilized by a sterile filter element is introduced; refined steam is introduced into the refined steam main pipeline, and high-temperature steam is introduced into the rough steam main pipeline and used for carrying out high-temperature sterilization on the culture materials.
The culture tank bottom is equipped with intake pipe 4 with the 3 intercommunication of main air inlet pipeline, and intake pipe 4 is equipped with first ooff valve 5 and first governing valve 6, and first ooff valve 5 is used for controlling switching on and shutting off of intake pipe 4, and first governing valve 6 is used for adjusting air intake flow. At least two sets of air inlet pipes 4, first switch valves 5 and first regulating valves 6 of the culture tank are arranged. The main air inlet pipeline 3 is communicated with the main air pipeline through an air inlet pipeline 7, and the air inlet pipeline 7 is provided with a second switch valve 8 for controlling the connection and disconnection of the air inlet pipeline 7. The primary air inlet pipeline 3 is provided with a third switching valve 9 for controlling the conduction and the disconnection of the primary air inlet pipeline 3. The main air inlet pipeline 3 is communicated with the refined steam main pipeline through a refined steam inlet pipeline 10, and the refined steam inlet pipeline 10 is provided with a fourth switch valve 11 for controlling the connection and disconnection of the refined steam inlet pipeline 10.
The bottom of the culture tank is also provided with a discharge pipe 12, the discharge pipe 12 is communicated with a crude steam main pipeline through a discharge sterilizing pipe 13, the discharge sterilizing pipe 13 is provided with a fifth switch valve 14, and the discharge pipe 12 is respectively provided with a sixth switch valve 15 and a seventh switch valve 16 above and below the communication position with the discharge sterilizing pipe 13. The fifth switching valve 14 is used to control whether the tapping pipe 12 is autoclaved using raw steam. When the sixth switch valve 15 and the seventh switch valve 16 are opened simultaneously, the material in the culture tank is removed from the discharge port. When the sixth on-off valve 15 is closed and the seventh on-off valve 16 is opened, the outlet of the tapping pipe can be sterilized at high temperature by opening the fifth on-off valve 14.
The culture tank body is provided with a barometer 53 for measuring and displaying the air pressure in the tank.
In this embodiment, the cultivation jar side is equipped with sample connection 17, and sample connection 17 is equipped with the sample connection sterile line 18 with thick steam trunk line intercommunication, is equipped with eighth ooff valve 19 on the sample connection sterile line. The sampling port 17 can be sterilized by opening the eighth on-off valve 19.
In this embodiment, the main air intake pipeline 3 is further communicated with the crude steam main pipeline through a crude steam air intake pipeline 20, and the crude steam air intake pipeline 20 is provided with a ninth switch valve 21 for controlling the connection and disconnection of the crude steam air intake pipeline 20. A small exhaust pipe 22 is arranged at the communication part of the coarse steam inlet pipeline 20 and the main inlet pipeline 3, and the small exhaust pipe 22 is provided with a tenth switch valve 23. The small exhaust pipe 22 is arranged to perform thorough high-temperature steam sterilization on the main air inlet pipeline 3, and a tenth switch valve 23 on the small exhaust pipe 22 is opened, so that steam can pass through the front pipeline, and dead angles during sterilization are avoided.
In this embodiment, the primary air inlet line is provided with a filter 24 for filtering impurities and germs in the air introduced into the culture tank. The filter 24 may be configured as a two-stage filter.
In this embodiment, the air inlet pipeline 7 is further provided with a flow meter 25 and an eleventh switch valve 26, and the flow meter 25 is installed between the second switch valve 8 and the eleventh switch valve 26 for displaying the flow rate of air introduced into the culture tank. The eleventh switching valve 26 is used to control the opening and closing of the air intake line 7.
In this embodiment, the top of the culture tank is provided with a tail gas pipe 27 for discharging gas in the culture tank. The tail gas pipe 27 is provided with a twelfth switch valve 28 for controlling the on and off of the tail gas pipe 27.
In this embodiment, as shown in fig. 2, the side wall of the culture tank is provided with an interlayer, the side surface of the culture tank is provided with an interlayer water inlet pipe 29 and an interlayer water outlet pipe 30, and the system further includes a cold water inlet main pipe, a cold water return main pipe, a low temperature water inlet main pipe, a low temperature water return main pipe, a hot water inlet main pipe and a hot water return main pipe, which are used for introducing normal temperature cold water, low temperature water and hot water into the interlayer, so as to control the temperature of the internal environment of the culture tank.
The interlayer water inlet pipe 29 is communicated with a hot water inlet main pipeline through a hot water inlet pipe 31, the hot water inlet pipe 31 is provided with a second regulating valve 32 and a thirteenth switch valve 33, the interlayer water inlet pipe 29 is communicated with a low-temperature water inlet main pipeline through a low-temperature water inlet pipe 34, the low-temperature water inlet pipe 34 is provided with a third regulating valve 35, a fourteenth switch valve 36 and a fifteenth switch valve 37, the low-temperature water inlet pipe 34 is communicated with a cold water inlet main pipeline through a cold water inlet pipe 38, the communication position of the cold water inlet pipe 38 and the low-temperature water inlet pipe 34 is located between the fourteenth switch valve 36 and the fifteenth switch valve 37, and the cold water inlet pipe 38 is provided with a sixteenth switch valve 39.
When the thirteenth on-off valve 33 is opened and the fourteenth on-off valve 36 is closed, hot water can be injected into the jacket through the hot water inlet main pipe, and the water flow rate can be adjusted by the second adjusting valve 32. When the thirteenth switching valve 33 is closed, the fourteenth switching valve 36 is opened, the fifteenth switching valve 37 is opened, and the sixteenth switching valve 39 is closed, low-temperature water can be injected into the interlayer through the low-temperature water inlet main pipe, and the water flow rate can be adjusted by the third adjusting valve 35. When the thirteenth switch valve 33 is closed, the fourteenth switch valve 36 is opened, the fifteenth switch valve 37 is closed, and the sixteenth switch valve 39 is opened, normal temperature cold water can be injected into the interlayer through the cold water inlet main pipe, and the water flow can be adjusted through the third adjusting valve 35.
The interlayer water outlet pipe 30 is communicated with a hot water main return pipe through a hot water return pipe 40, the hot water return pipe 40 is provided with a fourth regulating valve 41 and a seventeenth switch valve 42, the interlayer water outlet pipe 30 is communicated with a low-temperature water main return pipe through a low-temperature water return pipe 43, the low-temperature water return pipe 43 is provided with a fifth regulating valve 44, an eighteenth switch valve 45 and a nineteenth switch valve 46, the low-temperature water return pipe 43 is communicated with a cold water main return pipe through a cold water return pipe 47, the communication position of the cold water return pipe 47 and the low-temperature water return pipe 43 is positioned between the eighteenth switch valve 45 and the nineteenth switch valve 46, and the cold water return pipe 47 is provided with a twentieth switch valve.
When the seventeenth on-off valve 42 is opened and the eighteenth on-off valve 45 is closed, the hot water in the interlayer can be discharged through the hot water return main, and the water flow rate can be adjusted by the fourth adjusting valve 41. When the seventeenth on-off valve 42 is closed, the eighteenth on-off valve 45 is opened, the nineteenth on-off valve 46 is opened, and the twentieth on-off valve 48 is closed, the low-temperature water in the interlayer can be discharged through the low-temperature water return main pipe, and the water flow rate can be adjusted by the fifth adjusting valve 44. When the seventeenth on-off valve 42 is closed, the eighteenth on-off valve 45 is opened, the nineteenth on-off valve 46 is closed, and the twentieth on-off valve 48 is opened, the cold water at normal temperature in the interlayer can be discharged through the cold water return main pipe, and the water flow rate can be adjusted by the third adjusting valve 35.
In this embodiment, the interlayer water outlet pipe 30 is provided with an interlayer sterilizing pipe 49 communicated with the primary steam main pipe, and the interlayer sterilizing pipe 49 is provided with a twenty-first switch valve 50. Opening twenty-first ooff valve 50 can inject high temperature steam into the intermediate layer thereby disinfect the intermediate layer to and improve the culture tank jar body temperature.
In this embodiment, the culture tank includes a seeding tank 1 and a cell culture tank 2, the number of the seeding tank 1 is one, and the number of the cell culture tank 2 is two or more. The seeding tank 1 is used for primary plant amplification, and the cell culture tank 2 is used for secondary plant tissue cell amplification. The discharge pipe of the seeding tank 1 is connected with the feed inlet of the cell culture tank 2, and the material is pushed into the cell culture tank 2 through the pressure in the seeding tank 1.
The discharging pipes 12 of the cell culture tanks 2 are all communicated with a discharging main pipeline 51, the discharging main pipeline 51 is provided with a sixth regulating valve 52, and the sixth regulating valve 52 is used for regulating the discharging flow rate.
As shown in the attached figure 3, four sets of air inlet pipes 4, first switch valves 5 and first regulating valves 6 of the cell culture tank 2 are arranged, so that sufficient air can be conveniently introduced for plant tissue cell culture.
To sum up, the embodiment of the plant cell culture tank system provided by the utility model introduces pure air into the culture tank through the main air pipeline, can regulate and control the air flow introduced into the culture tank, can provide proper ventilation volume for plant cell culture, and measures and displays the pressure in the tank through the barometer on the tank body; the culture tank is provided with an interlayer which is controllably communicated with the cold water inlet main pipeline, the cold water return main pipeline, the low-temperature water inlet main pipeline, the low-temperature water return main pipeline, the hot water inlet main pipeline and the hot water return main pipeline, the temperature of the tank body can be flexibly controlled, and the proper environment temperature can be provided for plant cell culture; can carry out high temperature sterilization to the discharging pipe of cultivateing the jar, sample connection, these positions with external environment contact of air inlet pipeline and intermediate layer through thick steam trunk line for cultivateing jar lets in thick steam, and the setting of little blast pipe can carry out thorough high temperature steam sterilization to main air inlet pipeline on the main air inlet pipeline, and dead angle when avoiding the sterilization can provide no miscellaneous fungus environment for plant cell culture.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.
Claims (10)
1. The plant cell culture tank system is characterized by comprising a plurality of culture tanks, an air main pipeline, a fine steam main pipeline and a coarse steam main pipeline; the bottom of the culture tank is provided with an air inlet pipe (4) communicated with a main air inlet pipeline (3), the air inlet pipe (4) is provided with a first switch valve (5) and a first regulating valve (6), the main air inlet pipeline (3) is communicated with the main air pipeline through an air inlet pipeline (7), the air inlet pipeline (7) is provided with a second switch valve (8), the main air inlet pipeline (3) is provided with a third switch valve (9), the main air inlet pipeline (3) is communicated with the main fine steam pipeline through a fine steam inlet pipeline (10), and the fine steam inlet pipeline (10) is provided with a fourth switch valve (11); cultivate tank bottoms portion and still be equipped with discharging pipe (12), discharging pipe (12) through ejection of compact sterilization pipe (13) with thick steam trunk line intercommunication, ejection of compact sterilization pipe (13) are equipped with fifth ooff valve (14), discharging pipe (12) with the top and the below of the intercommunication department of ejection of compact sterilization pipe (13) are equipped with sixth ooff valve (15) and seventh ooff valve (16) respectively, cultivate the jar body and be equipped with barometer (53).
2. The plant cell culture tank system according to claim 1, wherein a sampling port (17) is provided on a side of the culture tank, the sampling port (17) is provided with a sampling port sterilizing pipe (18) communicated with the main crude steam pipe, and the sampling port sterilizing pipe is provided with an eighth switch valve (19).
3. The plant cell culture tank system according to claim 1, wherein the primary air inlet pipeline (3) is further communicated with the primary crude steam pipeline through a crude steam inlet pipeline (20), the crude steam inlet pipeline (20) is provided with a ninth switch valve (21), a small exhaust pipe (22) is arranged at the communication position of the crude steam inlet pipeline (20) and the primary air inlet pipeline (3), and the small exhaust pipe (22) is provided with a tenth switch valve (23).
4. A plant cell culture tank system according to claim 1, wherein the primary air inlet line is provided with a filter (24).
5. Plant cell culture tank system according to claim 1, wherein the air inlet line (7) is further provided with a flow meter (25) and an eleventh on-off valve (26), the flow meter (25) being mounted between the second on-off valve (8) and the eleventh on-off valve (26).
6. The plant cell culture tank system according to claim 1, wherein a tail gas pipe (27) is provided on the top of the culture tank, and the tail gas pipe (27) is provided with a twelfth on-off valve (28).
7. The plant cell culture tank system of claim 2, wherein the system further comprises a cold water inlet manifold, a cold water return manifold, a low temperature water inlet manifold, a low temperature water return manifold, a hot water inlet manifold and a hot water return manifold; the side wall of the culture tank is provided with an interlayer, and the side surface is provided with an interlayer water inlet pipe (29) and an interlayer water outlet pipe (30); the interlayer water inlet pipe (29) is communicated with a hot water inlet main pipe through a hot water inlet pipe (31), the hot water inlet pipe (31) is provided with a second regulating valve (32) and a thirteenth switch valve (33), the interlayer water inlet pipe (29) is communicated with a low-temperature water inlet main pipe through a low-temperature water inlet pipe (34), the low-temperature water inlet pipe (34) is provided with a third regulating valve (35), a fourteenth switch valve (36) and a fifteenth switch valve (37), the low-temperature water inlet pipe (34) is communicated with a cold water inlet main pipe through a cold water inlet pipe (38), the communication position of the cold water inlet pipe (38) and the low-temperature water inlet pipe (34) is located between the fourteenth switch valve (36) and the fifteenth switch valve (37), and the cold water inlet pipe (38) is provided with a sixteenth switch valve (39); intermediate layer outlet pipe (30) return main pipeline intercommunication with hot water through hot water return pipe (40), hot water return pipe (40) is equipped with fourth governing valve (41) and seventeenth ooff valve (42), intermediate layer outlet pipe (30) return pipe (43) and low temperature water through low warm water and return main pipeline intercommunication, low temperature water return pipe (43) are equipped with fifth governing valve (44), eighteenth ooff valve (45) and nineteenth ooff valve (46), low temperature water return pipe (43) return pipe (47) and cold water and return main pipeline intercommunication, cold water return pipe (47) with the intercommunication department of low temperature water return pipe (43) is located eighteenth ooff valve (45) with the centre of nineteenth ooff valve (46), cold water return pipe (47) are equipped with twentieth ooff valve (48).
8. A plant cell culture tank system according to claim 7, wherein the mezzanine water outlet pipe (30) is provided with a mezzanine sterilizing pipe (49) communicating with the primary steam main pipe, the mezzanine sterilizing pipe (49) being provided with a twenty-first on-off valve (50).
9. The plant cell culture tank system according to claim 1, wherein the culture tank comprises a seeding tank (1) and a cell culture tank (2), the number of the seeding tank (1) is one, and the number of the cell culture tank (2) is two or more.
10. The plant cell culture tank system is characterized by comprising a plurality of culture tanks, an air main pipeline, a fine steam main pipeline and a coarse steam main pipeline; the bottom of the culture tank is provided with an air inlet pipe (4) communicated with a main air inlet pipeline (3), the air inlet pipe (4) is provided with a first switch valve (5) and a first regulating valve (6), the main air inlet pipeline (3) is communicated with the main air pipeline through an air inlet pipeline (7), the air inlet pipeline (7) is provided with a second switch valve (8), the main air inlet pipeline (3) is provided with a third switch valve (9), the main air inlet pipeline (3) is communicated with the main fine steam pipeline through a fine steam inlet pipeline (10), and the fine steam inlet pipeline (10) is provided with a fourth switch valve (11); the bottom of the culture tank is also provided with a discharge pipe (12), the discharge pipe (12) is communicated with the crude steam main pipeline through a discharge sterilizing pipe (13), the discharge sterilizing pipe (13) is provided with a fifth switch valve (14), the discharge pipe (12) is respectively provided with a sixth switch valve (15) and a seventh switch valve (16) above and below the communication position of the discharge sterilizing pipe (13), and the culture tank body is provided with a barometer (53);
a sampling port (17) is arranged on the side surface of the culture tank, a sampling port sterilizing pipe (18) communicated with the crude steam main pipeline is arranged on the sampling port (17), and an eighth switch valve (19) is arranged on the sampling port sterilizing pipe;
the main air inlet pipeline (3) is also communicated with the crude steam main pipeline through a crude steam inlet pipeline (20), the crude steam inlet pipeline (20) is provided with a ninth switch valve (21), a small exhaust pipe (22) is arranged at the communication position of the crude steam inlet pipeline (20) and the main air inlet pipeline (3), and the small exhaust pipe (22) is provided with a tenth switch valve (23);
the primary air inlet pipeline is provided with a filter (24);
the air inlet pipeline (7) is also provided with a flow meter (25) and an eleventh switch valve (26), and the flow meter (25) is arranged between the second switch valve (8) and the eleventh switch valve (26);
a tail gas pipe (27) is arranged at the top of the culture tank, and a twelfth switch valve (28) is arranged on the tail gas pipe (27);
the system also comprises a cold water inlet main pipeline, a cold water return main pipeline, a low-temperature water inlet main pipeline, a low-temperature water return main pipeline, a hot water inlet main pipeline and a hot water return main pipeline; the side wall of the culture tank is provided with an interlayer, and the side surface is provided with an interlayer water inlet pipe (29) and an interlayer water outlet pipe (30); the interlayer water inlet pipe (29) is communicated with a hot water inlet main pipe through a hot water inlet pipe (31), the hot water inlet pipe (31) is provided with a second regulating valve (32) and a thirteenth switch valve (33), the interlayer water inlet pipe (29) is communicated with a low-temperature water inlet main pipe through a low-temperature water inlet pipe (34), the low-temperature water inlet pipe (34) is provided with a third regulating valve (35), a fourteenth switch valve (36) and a fifteenth switch valve (37), the low-temperature water inlet pipe (34) is communicated with a cold water inlet main pipe through a cold water inlet pipe (38), the communication position of the cold water inlet pipe (38) and the low-temperature water inlet pipe (34) is located between the fourteenth switch valve (36) and the fifteenth switch valve (37), and the cold water inlet pipe (38) is provided with a sixteenth switch valve (39); the interlayer water outlet pipe (30) is communicated with a hot water main return pipe through a hot water main return pipe (40), the hot water main return pipe (40) is provided with a fourth regulating valve (41) and a seventeenth switch valve (42), the interlayer water outlet pipe (30) is communicated with a low-temperature water main return pipe through a low-temperature water main return pipe (43), the low-temperature water main return pipe (43) is provided with a fifth regulating valve (44), an eighteenth switch valve (45) and a nineteenth switch valve (46), the low-temperature water main return pipe (43) is communicated with a cold water main return pipe through a cold water main return pipe (47), the communication position of the cold water main return pipe (47) and the low-temperature water main return pipe (43) is positioned between the eighteenth switch valve (45) and the nineteenth switch valve (46), and the cold water main return pipe (47) is provided with a twentieth switch valve (48);
the interlayer water outlet pipe (30) is provided with an interlayer sterilizing pipe (49) communicated with the primary steam main pipeline, and the interlayer sterilizing pipe (49) is provided with a twenty-first switch valve (50);
the culture tank comprises a seeding tank (1) and cell culture tanks (2), wherein the number of the seeding tank (1) is one, and the number of the cell culture tanks (2) is two or more.
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| CN201922453171.0U CN211947072U (en) | 2019-12-30 | 2019-12-30 | Plant cell culture tank system |
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| CN201922453171.0U CN211947072U (en) | 2019-12-30 | 2019-12-30 | Plant cell culture tank system |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117256481A (en) * | 2023-11-22 | 2023-12-22 | 上海科缔联科技有限公司 | Pipeline structure of continuous culture device |
| CN117281045A (en) * | 2023-11-22 | 2023-12-26 | 上海科缔联科技有限公司 | Temperature regulating system of plant tissue culture tank |
| CN117338968A (en) * | 2023-11-22 | 2024-01-05 | 山东安然纳米实业发展有限公司 | A steam sterilization cycle for plant tissue culture tanks |
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2019
- 2019-12-30 CN CN201922453171.0U patent/CN211947072U/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117256481A (en) * | 2023-11-22 | 2023-12-22 | 上海科缔联科技有限公司 | Pipeline structure of continuous culture device |
| CN117281045A (en) * | 2023-11-22 | 2023-12-26 | 上海科缔联科技有限公司 | Temperature regulating system of plant tissue culture tank |
| CN117338968A (en) * | 2023-11-22 | 2024-01-05 | 山东安然纳米实业发展有限公司 | A steam sterilization cycle for plant tissue culture tanks |
| CN117281045B (en) * | 2023-11-22 | 2024-02-13 | 上海科缔联科技有限公司 | Temperature regulating system of plant tissue culture tank |
| CN117256481B (en) * | 2023-11-22 | 2024-02-27 | 上海科缔联科技有限公司 | Pipeline structure of continuous culture device |
| CN117338968B (en) * | 2023-11-22 | 2024-12-03 | 山东安然纳米实业发展有限公司 | Steam disinfection circulation loop of plant tissue culture tank |
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