CN216237091U

CN216237091U - Auxiliary device for appearance inspection of oocyte and embryo incubator

Info

Publication number: CN216237091U
Application number: CN202122885562.7U
Authority: CN
Inventors: 张燕; 马庆涛; 侯明海; 王玲玲; 高运东
Original assignee: Shandong Ox Livestock Breeding Co Ltd
Current assignee: Shandong Ox Livestock Breeding Co Ltd
Priority date: 2021-11-23
Filing date: 2021-11-23
Publication date: 2022-04-08
Anticipated expiration: 2031-11-23

Abstract

The utility model relates to the technical field of cell and embryo culture, in particular to an auxiliary device for observing the appearance of an oocyte and embryo incubator, which comprises an observation device, a first constant-temperature chamber and a second constant-temperature chamber, wherein the observation device consists of a microscope and the first transparent constant-temperature chamber for placing a culture dish; a temporary storage device having a second transparent thermostatic chamber in which a plurality of culture dishes to be observed are placed; the gas supply and humidification device supplies humidified gas for culturing the oocyte and the embryo to the first transparent thermostatic chamber and the second transparent thermostatic chamber; the utility model meets the requirements of the oocyte and the embryo on temperature, humidity and gas concentration in the observation and operation processes outside the incubator, reduces the environmental fluctuation in the oocyte and the embryo incubator, and reduces the bad stimulation to the development of the oocyte and the embryo.

Description

Auxiliary device for appearance inspection of oocyte and embryo incubator

Technical Field

The utility model relates to the technical field of cell and embryo culture, in particular to an auxiliary device for appearance observation of an oocyte and embryo incubator.

Background

The statements herein merely provide background information related to the present disclosure and may not necessarily constitute prior art.

The in vitro culture of oocytes and embryos is a key link in the in vitro embryo production technology, and the growth environment of the oocytes and the embryos directly influences the growth and development of early embryos and further influences the conception rate of the transplanted embryos. The in vitro culture of oocytes and embryos requires a sterile environment that provides a certain temperature, humidity and gas concentration, and therefore the culture of oocytes and embryos needs to be performed in an incubator.

However, during the culture process, the oocyte and the embryo are inevitably required to be taken out of the incubator, and the opening and closing of the door of the incubator can cause the fluctuation of the environment in the incubator, thereby influencing the development of the culture in the incubator. Especially, when the cultured oocytes and embryos are in multiple batches, the stability of the culture environment in the development process of the oocytes and the embryos cannot be ensured by frequently opening and closing the doors of the incubator. And the oocytes and embryos outside the incubator may also have their development affected due to environmental changes such as lack of gas supply. Although the development process of oocytes and embryos can be continuously monitored for 24 hours by using an advanced delayed photographic culture system and depending on a microscope and a camera in an incubator, the number of times and time for taking the oocytes and embryos out of the incubator to observe are reduced, the system is expensive, and the production cost of in vitro embryos can be greatly increased.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide an auxiliary device for observing the appearance of an oocyte and an embryo incubator, and aims to solve the problems in the prior art. In order to achieve the above object, the present invention is achieved by the following technical solutions:

the utility model provides an auxiliary device for appearance inspection of an oocyte and embryo incubator, which comprises:

the observation device consists of a microscope and a first transparent thermostatic chamber for placing a culture dish, and the microscope is used for observing the culture dish through the first transparent thermostatic chamber;

a temporary storage device having a second transparent thermostatic chamber in which a plurality of culture dishes to be observed are placed;

and the gas supply and humidification device is used for supplying humidified gas for culturing the oocyte and the embryo to the first transparent thermostatic chamber and the second transparent thermostatic chamber.

As a further technical solution, the gas supply and humidification device comprises and communicates with a gas supply container and a gas humidifier, which communicates with the first transparent thermostatic chamber and the second transparent thermostatic chamber, respectively.

As a further technical scheme, the first transparent constant temperature chamber consists of a transparent constant temperature table and a first transparent cover, and the open end of the first transparent cover is buckled on the transparent constant temperature table.

As a further technical scheme, the second transparent constant temperature chamber consists of a constant temperature table and a second transparent cover, and the open end of the second transparent cover is buckled on the constant temperature table.

As a further technical scheme, a gas filtering column is arranged between the gas supply container and the gas humidifier.

As a further technical scheme, the gas filtering column is respectively communicated with the gas supply container and the gas humidifier through silica gel hoses, and the gas humidifier is communicated with the first transparent constant temperature chamber and the second transparent constant temperature chamber through silica gel hoses with three-way valves.

As a further technical scheme, the transparent constant temperature table is a glass heating table.

As a further technical scheme, the first transparent cover is a glass round cover.

As a further technical scheme, the second transparent cover is a glass round cover.

As a further technical solution, the microscope is a stereoscopic microscope.

The beneficial effects of the utility model are as follows:

(1) humidified gas is introduced into the first transparent constant-temperature chamber and the second transparent constant-temperature chamber, so that the requirements of the oocyte and the embryo on temperature, humidity and gas concentration in the processes of observation and operation outside the incubator are met.

(2) The second transparent constant temperature chamber can temporarily store a plurality of culture dishes, the culture dishes are taken out of the second transparent constant temperature chamber and placed into the first transparent constant temperature chamber when being observed, the second transparent constant temperature chamber provides a temporary storage area for the culture dishes to be observed, the adverse effect of opening and closing the door of the culture box for many times on the environment in the box is avoided, the environmental fluctuation in the oocyte and embryo incubator is reduced, and the adverse stimulation on the development of the oocyte and embryo is reduced.

(3) The utility model has the advantages of simple structure, convenient installation, simple and convenient operation and low cost.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the utility model, and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description, serve to explain the utility model and not to limit the utility model. It will be further appreciated that the drawings are for simplicity and clarity and have not necessarily been drawn to scale. The utility model will now be described and explained with additional specificity and detail through the use of the accompanying drawings in which:

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic view of the structure of the gas humidifier of the present invention.

In the figure: 10 gas supply container, 11 gas tank, 12 pressure reducing valve, 20 gas humidifier, 21 humidifying cup, 22 gushing gas pipe, 23 highest water level line, 24 lowest water level line, 25 gas outlet, 26 gas inlet, 27 gas flow adjusting knob, 28 gas flow buoy, 30 observation device, 31 microscope, 32 transparent constant temperature table, 33 first transparent cover, 34 culture dish, 40 temporary storage device, 41 constant temperature table, 42 second transparent cover, 43 culture dish to be observed, 50 gas filter column, 60 first silica gel hose, 70 second silica gel hose, 80 third silica gel hose, 90 three-way valve, 100 fourth silica gel hose and 110 fifth silica gel hose.

Detailed Description

The technical solution in an exemplary embodiment of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiment of the present invention.

As shown in fig. 1-2, the present embodiment provides an auxiliary device for observing the appearance of an oocyte and embryo incubator, including:

an observation device 30 which is composed of a microscope 31 and a first transparent thermostatic chamber for placing the culture dish 34, wherein the microscope 31 observes the culture dish 34 through the first transparent thermostatic chamber;

a temporary storage device 40 having a second transparent thermostatic chamber in which a plurality of culture dishes 43 to be observed are placed;

and the gas supply and humidification device supplies humidified gas for culturing the oocytes and embryos to the first transparent thermostatic chamber and the second transparent thermostatic chamber.

Wherein the first transparent constant temperature chamber and the second transparent constant temperature chamber are filled with humidified gas, so that the requirements of the oocyte and the embryo on temperature, humidity and gas concentration in the process of observation and operation outside the incubator are met.

In addition, the transparent constant temperature chamber of second can deposit a plurality ofly temporarily and wait to observe culture dish 43, takes out from it and puts into first transparent constant temperature chamber when observing, and the transparent constant temperature chamber of second provides a temporary storage area for waiting to observe culture dish 43, has avoided the harmful effects of many times switch incubator door to the incasement environment production, has reduced the environmental fluctuation in oocyte and the embryo culture case, has reduced the bad stimulus to oocyte and embryonic development.

Preferably, the microscope 31 is a stereo microscope, also known as a solid microscope, a binocular microscope that views an object from different angles, causing two eyes to perceive a stereoscopic sensation. In the observation process, the first transparent chamber does not need to be opened, the culture dish does not need to be processed and manufactured, and the culture dish can be observed by being directly placed under the lens and matched with illumination, like being upright.

Further, the first transparent constant temperature chamber is composed of a transparent constant temperature table 32 and a first transparent cover 33, the open end of the first transparent cover 33 is buckled on the transparent constant temperature table, preferably, the transparent constant temperature table 32 is a glass heating table, the glass heating table does not obstruct the illumination of the microscope 31, the glass heating table is the prior art, and details are not repeated here. Of course, in other embodiments, other transparent heating platforms may be used. The second transparent thermostatic chamber is composed of a thermostatic table 41 and a second transparent cover 42, and the open end of the second transparent cover 42 is buckled on the thermostatic table 41. The thermostatic stage 41 is a common heating platform, for which no transparency is required, since the main function of the second transparent thermostatic chamber is not to observe but to store it temporarily.

Preferably, the first transparent cover 33 is a glass round cover, and the second transparent cover 42 is a glass round cover, and all the materials are medical sterilization grade or sterilizable materials, so as to ensure the sterility of the culture process. And can be changed, cleaned, disinfected and sterilized independently, greatly reducing the cultivation cost and the possibility of pollution in the cultivation process. In other embodiments, a square cover or a triangular cover may be used.

In the present embodiment, the air inlets are disposed on the top of the first transparent cover 33 and the second transparent cover 42.

Further, the gas supply and humidification device comprises a gas supply container 10 and a gas humidifier 20 which are communicated, and a gas filter column 50 is arranged between the gas supply container 10 and the gas humidifier 20. The gas filtering column 50 is a gas filtering device containing a HEPA filter and active carbon components, can remove volatile organic compounds, microorganisms, particles and the like in gas, and provides a sterile culture environment for oocyte and embryo culture. The gas humidifier 20 is in communication with the first and second transparent thermostatic chambers, respectively.

The gas filtering column 50 is respectively communicated with the gas supply container 10 and the gas humidifier 20 through silica gel hoses, and the gas humidifier 20 is communicated with the first transparent constant temperature chamber and the second transparent constant temperature chamber through the silica gel hoses with the three-way valves 90.

In the present embodiment, the gas supply container 10 includes a pressure reducing valve 12 and a gas tank 11. The gas tank 11 is filled with mixed gas consisting of gases with different concentrations required for culturing oocytes and embryos.

The air humidifier 20 is an integrated air inhaler and includes an air flow float 28, an air flow adjustment knob 27, a humidifying cup 21, a surge pipe 22, an air inlet 26 and an air outlet 25. The scale indicated by the float in the gas flow float 28 is a gas flow reading in liters/minute. The gas flow adjustment knob 27 enables the magnitude of the gas flow through the device to be adjusted. The tide cup 21 is marked with a highest water level line 23 and a lowest water level line 24.

The pressure reducing valve 12 is communicated with the gas filtering column 50 through a fifth silica gel hose 110, the gas filtering column 50 is communicated with the gas inlet 26 of the gas humidifier 20 through a fourth silica gel hose 100, the gas outlet 25 of the gas filtering column is connected with a third silica gel hose 80, the other end of the third silica gel hose 80 is connected with the inlet of the three-way valve 90, two outlets of the three-way valve 90 are respectively connected with the first silica gel hose 60 and the second silica gel hose 70, and the first silica gel hose 60 and the second silica gel hose 70 are respectively connected with the gas inlets at the tops of the first transparent cover 33 and the second transparent cover 42.

In this embodiment, the silicone hose is a medical silicone hose with an inner diameter of 6mm and a wall thickness of 2 mm.

Before the incubator appearance inspection auxiliary device is used, a gas supply passage is required to be closed, and the gas supply passage comprises a gas tank 11 of a gas supply container 10, a pressure reducing valve 12, a gas flow adjusting knob 27 of a gas humidifier 20 and a valve of a three-way valve 90. The transparent constant temperature table 32 and the constant temperature table 41 are opened in advance, after the temperature is raised to the required temperature, the humidifying cup 21 is opened, the sterilized distilled water is injected in advance, the water level in the bottle is positioned between the highest water level line 23 and the lowest water level line 24, and the cup body is screwed tightly. The valve of the gas tank 11 is opened, the pressure reducing valve 12 is rotated counterclockwise, then the flow adjusting knob 27 is rotated counterclockwise, so that the gas flows into the humidifying cup 21 through the surge pipe 22, the gas is humidified by the sterilized distilled water therein, and then the valve of the three-way valve 90 is opened, so that the humidified gas is injected into the first transparent cover 33 and the second transparent cover 42 through the silica gel hose. The oocytes and embryos to be observed and manipulated are all removed from the incubator, one of the culture dishes 34 is placed on the transparent thermostatic stage 32, and the transparent thermostatic stage 32 is placed under the lens of the microscope 31. And placing the redundant culture dishes 43 to be observed on the constant temperature table 41, covering the second transparent cover 42 filled with the mixed gas on the culture dishes 43 to be observed, and sequentially taking, placing and observing until all the culture dishes are observed.

Although the present invention has been described with reference to the preferred embodiments, it is not intended to limit the present invention, and those skilled in the art can make variations and modifications of the present invention without departing from the spirit and scope of the present invention by using the methods and technical contents disclosed above.

Claims

1. The utility model provides an auxiliary device is observed to oocyte and embryo incubator outward appearance which characterized in that includes:

2. The oocyte and embryo incubator appearance inspection auxiliary device according to claim 1, wherein the gas supply and humidification device includes a gas supply container and a gas humidifier, and the gas humidifier is respectively communicated with the first transparent thermostatic chamber and the second transparent thermostatic chamber.

3. The visual inspection assisting device for the oocyte and embryo incubator according to claim 2, wherein the first transparent thermostatic chamber is composed of a transparent thermostatic table and a first transparent cover, and an open end of the first transparent cover is buckled on the transparent thermostatic table.

4. The visual inspection aid for oocyte and embryo incubators according to claim 2, wherein the second transparent thermostatic chamber is composed of a thermostatic stage and a second transparent cover, and an open end of the second transparent cover is fastened on the thermostatic stage.

5. The visual inspection assisting device for the oocyte and embryo incubator according to claim 2, wherein a gas filter column is disposed between the gas supply container and the gas humidifier.

6. The visual inspection assisting device for the oocyte and embryo incubator as claimed in claim 5, wherein the gas filtering column is connected to the gas supply container and the gas humidifier through silicone hoses, and the gas humidifier is connected to the first transparent thermostatic chamber and the second transparent thermostatic chamber through silicone hoses with three-way valves.

7. The visual inspection assisting device for the oocyte and embryo incubator according to claim 3, wherein the transparent constant temperature stage is a glass heating stage.

8. The visual inspection aid of an oocyte and embryo incubator according to claim 3, wherein the first transparent cover is a glass dome.

9. The visual inspection aid of an oocyte and embryo incubator according to claim 4, wherein the second transparent cover is a glass dome.

10. The visual inspection assisting device for the oocyte and embryo culture box according to any one of claims 1 to 7, wherein the microscope is a stereoscopic microscope.