CN211367585U - Butt-joint carbon dioxide culture system - Google Patents
Butt-joint carbon dioxide culture system Download PDFInfo
- Publication number
- CN211367585U CN211367585U CN201921759830.7U CN201921759830U CN211367585U CN 211367585 U CN211367585 U CN 211367585U CN 201921759830 U CN201921759830 U CN 201921759830U CN 211367585 U CN211367585 U CN 211367585U
- Authority
- CN
- China
- Prior art keywords
- air
- carbon dioxide
- pipe
- return
- valve
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a dock carbon dioxide culture system aims at solving carbon dioxide culture system and has the not enough of pollution and the disconnected confession risk of air supply. The utility model comprises a carbon dioxide incubator, an air source component, an isolator, a sterilizing gas generator and a controller, wherein the carbon dioxide incubator is communicated with an air source pipe and a sterilizing return air pipe, and the sterilizing return air pipe is connected with a return air main pipe; the air source pipe is provided with a sterilizing filter, the sterilizing filter is connected with an air inlet valve and an air outlet valve in parallel, the air outlet valve is connected with an air pump in series, and the air pump and a sterilizing air return pipe are connected with a return air main pipe in parallel; the air source assembly comprises a plurality of carbon dioxide air storage tanks, the carbon dioxide air storage tanks are connected in parallel on the air supply main pipe, the air pressure of the carbon dioxide air storage tanks is insufficient for automatic switching, the air supply main pipe is connected with the air inlet valve, a flow meter and a pressure reducing valve are installed on the air inlet main pipe, an environment monitoring sensor is installed in the carbon dioxide incubator, and the flow meter and the environment monitoring sensor are both electrically connected with the controller.
Description
Technical Field
The utility model relates to a carbon dioxide cultivates the technique, and more specifically says that it relates to a dock carbon dioxide cultivation system.
Background
The carbon dioxide incubator is a device for culturing cells or tissues in vitro by simulating and forming a growth environment similar to cells or tissues in an organism in an incubator body, and the incubator requires stable temperature, stable carbon dioxide level, constant pH value and higher relative saturation humidity, is an advanced instrument for culturing cells, tissues and bacteria, and is key equipment necessary for developing immunology, oncology, genetics and biological engineering. At present, the cell operation for experiment is mostly completed in an ultra clean bench arranged in a clean room grade C area, and the cell culture is completed in a common incubator in the clean room grade C area. The inner cavities of the conventional incubators on the market are not closed, the problem that pipelines from an external filter to the cavities and carbon dioxide control valve cavities are not sterilized exists, and the pollution risk exists, so that the cell medicines produced do not accord with the production quality management specifications of medicines. In general, the cell and tissue culture needs a long time, namely a few weeks and a few months, and the air source is not allowed to be interrupted in the culture process. If the carbon dioxide gas source is insufficient during the culture process of the cells and the tissues, the cells and the tissues grow slowly or die. At present, cell culture operators manually check the air supply condition of an air source at regular intervals, and the culture process needs to be attended. If the culture process is unattended, there is a risk of carbon dioxide gas source outage.
SUMMERY OF THE UTILITY MODEL
The utility model overcomes carbon dioxide culture system has the not enough of pollution and the disconnected confession risk of air supply, provides a dock carbon dioxide culture system, and it can avoid polluting the disconnected risk of confession of air supply, guarantees the reliability of cultivation process, makes the cell medicine that produces accord with the pharmaceutical production quality control standard.
In order to solve the technical problem, the utility model discloses a following technical scheme: a butt-joint type carbon dioxide culture system comprises a carbon dioxide culture box, an air source assembly, an isolator connected with the carbon dioxide culture box, a sterilizing gas generator connected with the isolator and a controller, wherein the carbon dioxide culture box is hermetically connected with the isolator through a flange; the carbon dioxide incubator is communicated with an air source pipe and a sterilization air return pipe, and the sterilization air return pipe is connected with an air return main pipe; the air source pipe is provided with a sterilizing filter, the sterilizing filter is connected with an air inlet valve and an air outlet valve in parallel, the air outlet valve is connected with an air pump in series, and the air pump and a sterilizing air return pipe are connected with a return air main pipe in parallel; the air source assembly comprises a plurality of carbon dioxide air storage tanks, the carbon dioxide air storage tanks are connected in parallel on the air supply main pipe, the air pressure of the carbon dioxide air storage tanks is insufficient for automatic switching, the air supply main pipe is connected with the air inlet valve, a flow meter and a pressure reducing valve are installed on the air inlet main pipe, an environment monitoring sensor is installed in the carbon dioxide incubator, and the flow meter and the environment monitoring sensor are both electrically connected with the controller.
When the carbon dioxide culture system carries out sterilization operation, the sterilizing gas generator generates sterilizing gas, the sterilizing gas is sent into the isolator and then enters the carbon dioxide culture box for sterilization. The sterilizing gas flows into the air return main pipe through the sterilizing air return pipe and then is discharged. In addition, the air pump is started, so that the sterilized gas in the carbon dioxide incubator flows into the air source pipe, sequentially passes through the sterilizing filter, the exhaust valve and the air pump, flows into the air return header pipe and is discharged, the air source pipe is sterilized in the process, and the risk of pollution is reduced. The carbon dioxide gas storage tanks are arranged, the air pressure of the carbon dioxide gas storage tanks is insufficient, and automatic switching is achieved, so that the phenomenon that the air source is disconnected due to the arrangement of a single carbon dioxide gas storage tank is avoided. The environmental monitoring sensor through installation in the carbon dioxide incubator detects carbon dioxide concentration and carbon dioxide incubator internal pressure, and the data control admission valve that detects opens and closes, carries out aseptic filtration to carbon dioxide through the bacteria removal filter. The carbon dioxide culture system can avoid the risks of pollution and air source supply interruption, ensure the reliability of the culture process and ensure that the produced cell medicine conforms to the drug production quality management standard.
Preferably, the isolator is provided with a return air channel, the return air main pipe is communicated with the return air channel, and the return air main pipe is provided with a return air valve. The air return main pipe is communicated with an air return channel on the isolator, so that the installation and the arrangement of the air return main pipe are convenient.
Preferably, a stirring fan is installed in the carbon dioxide incubator. Stirring fan stirs the gas in the carbon dioxide incubator, makes the gaseous and carbon dioxide distribution of sterilization more even, and the sterilization is more thorough, and carbon dioxide concentration control is more accurate.
Preferably, the carbon dioxide incubator is communicated with a pressure compensation pipe, the pressure compensation pipe is communicated with the air return main pipe, a pressure compensation degerming filter and a pressure compensation pipeline valve are installed on the pressure compensation pipe, and a pressure compensation switch valve is connected between the pressure compensation degerming filter and the pressure compensation pipeline valve in parallel on the pressure compensation pipe.
When the pressure in the carbon dioxide incubator is detected to exceed the culture pressure, the pressure compensation switch valve is opened to release the pressure, and the normal pressure culture or the positive and negative pressure culture in the incubator is maintained.
Preferably, the air inlet valve, the exhaust valve, the pressure compensation pipeline valve and the pressure compensation switch valve are all electromagnetic valves and are all electrically connected with the controller. The electromagnetic valve is accurately and reliably controlled.
Preferably, a ventilation branch pipe is connected between the carbon dioxide gas storage tank and the gas supply main pipe, a pressure sensor and an air inlet electric control valve are mounted on the ventilation branch pipe, and the pressure sensor and the air inlet electric control valve are electrically connected with the controller.
The controller controls the on-off of a single ventilation branch pipe to realize the automatic switching of the air source, when the pressure sensor detects that the air pressure on the ventilation branch pipe is insufficient, the controller controls the air inlet electric control valve on the ventilation branch pipe to be closed, and simultaneously opens the air inlet electric control valve on the other ventilation branch pipe to realize the switching of the air source.
Compared with the prior art, the beneficial effects of the utility model are that: (1) the carbon dioxide culture system can avoid the risks of pollution and air source supply interruption, ensure the reliability of the culture process and ensure that the produced cell medicine conforms to the drug production quality management standard; (2) the air source is automatically switched, and the operation is convenient.
Drawings
Fig. 1 is a schematic structural view of the present invention;
in the figure: 1. carbon dioxide incubator, 2, isolator, 3, sterilization gas generator, 4, controller, 5, incubator door, 6, air supply pipe, 7, sterilization return air pipe, 8, return air main, 9, sterilization filter, 10, admission valve, 11, discharge valve, 12, aspiration pump, 13, carbon dioxide gas holder, 14, flowmeter, 15, relief pressure valve, 16, environmental monitoring sensor, 17, return air passageway, 18, stirring fan, 19, pressure compensation pipe, 20, pressure compensation sterilization filter, 21, pressure compensation pipeline valve, 22, pressure compensation switch valve, 23, air vent branch, 24, pressure sensor, 25, the automatically controlled valve that admits air.
Detailed Description
The technical solution of the present invention is further described in detail by the following specific embodiments in combination with the accompanying drawings:
example (b): a butt-joint type carbon dioxide culture system (see attached figure 1) comprises a carbon dioxide culture box 1, an air source assembly, an isolator 2 connected with the carbon dioxide culture box, a sterilizing gas generator 3 connected with the isolator and a controller 4, wherein the carbon dioxide culture box is arranged in a sealing mode, and the carbon dioxide culture box is connected with the isolator in a sealing mode through a flange. A communication window is arranged between the carbon dioxide incubator and the isolator, the carbon dioxide incubator is connected with an incubator door 5, and the incubator door is sealed and covered with the communication window; the carbon dioxide incubator is communicated with an air source pipe 6 and a sterilization air return pipe 7, and the sterilization air return pipe is connected with an air return main pipe 8; a sterilization filter 9 is arranged on the air source pipe, an air inlet valve 10 and an exhaust valve 11 are connected on the sterilization filter in parallel, the exhaust valve is connected with an air pump 12 in series, the air pump and a sterilization return air pipe are connected on a return air main pipe in parallel, and an isolation valve is arranged on the sterilization return air pipe; the air source assembly comprises a plurality of carbon dioxide air storage tanks 13, the carbon dioxide air storage tanks are connected in parallel on the air supply main pipe, the air pressure of the carbon dioxide air storage tanks is insufficient for automatic switching, the air supply main pipe is connected with the air inlet valve, a flow meter 14 and a pressure reducing valve 15 are installed on the air supply main pipe, an environment monitoring sensor 16 is installed in the carbon dioxide incubator, and the flow meter and the environment monitoring sensor are both electrically connected with the controller. The isolator is provided with a return air channel 17, the return air main pipe is communicated with the return air channel, and the return air main pipe is provided with a return air valve. A stirring fan 18 is installed in the carbon dioxide incubator. The carbon dioxide incubator is communicated with a pressure compensation pipe 19, the pressure compensation pipe is communicated with the air return main pipe, a pressure compensation degerming filter 20 and a pressure compensation pipeline valve 21 are installed on the pressure compensation pipe, and a pressure compensation switch valve 22 is connected between the pressure compensation degerming filter and the pressure compensation pipeline valve in parallel on the pressure compensation pipe. The air inlet valve, the exhaust valve, the pressure compensation pipeline valve and the pressure compensation switch valve are all electromagnetic valves and are electrically connected with the controller.
And a ventilation branch pipe 23 is connected between the carbon dioxide gas storage tank and the gas supply main pipe, a pressure sensor 24 and an air inlet electric control valve 25 are arranged on the ventilation branch pipe, and the pressure sensor and the air inlet electric control valve are electrically connected with a controller.
When the carbon dioxide culture system carries out sterilization operation, the sterilization gas generator generates sterilization gas and sends the sterilization gas into the isolator, the incubator door is opened, and the sterilization gas enters the carbon dioxide incubator through the communication window for sterilization. The sterilizing gas flows into the air return main pipe through the sterilizing air return pipe and then is discharged. In addition, the air pump is started, so that the sterilized gas in the carbon dioxide incubator flows into the air source pipe, sequentially passes through the sterilizing filter, the exhaust valve and the air pump, flows into the air return header pipe and is discharged, the air source pipe is sterilized in the process, and the risk of pollution is reduced. All pipelines are sterilized in situ, and the sterilization effect is good. The carbon dioxide gas storage tanks are arranged, the air pressure of the carbon dioxide gas storage tanks is insufficient, and automatic switching is achieved, so that the phenomenon that the air source is disconnected due to the arrangement of a single carbon dioxide gas storage tank is avoided. The environmental monitoring sensor through installation in the carbon dioxide incubator detects carbon dioxide concentration and carbon dioxide incubator internal pressure, and the data control admission valve that detects opens and closes, carries out aseptic filtration to carbon dioxide through the bacteria removal filter. The carbon dioxide culture system can avoid the risks of pollution and air source supply interruption, ensure the reliability of the culture process and ensure that the produced cell medicine conforms to the drug production quality management standard.
The above-described embodiments are merely preferred and are not intended to limit the present invention in any way, and other variations and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (6)
1. A butt-joint type carbon dioxide culture system is characterized by comprising a carbon dioxide culture box, an air source assembly, an isolator connected with the carbon dioxide culture box, a sterilizing gas generator connected with the isolator and a controller, wherein the carbon dioxide culture box is hermetically connected with the isolator through a flange; the carbon dioxide incubator is communicated with an air source pipe and a sterilization air return pipe, and the sterilization air return pipe is connected with an air return main pipe; the air source pipe is provided with a sterilizing filter, the sterilizing filter is connected with an air inlet valve and an air outlet valve in parallel, the air outlet valve is connected with an air pump in series, and the air pump and a sterilizing air return pipe are connected with a return air main pipe in parallel; the air source assembly comprises a plurality of carbon dioxide air storage tanks, the carbon dioxide air storage tanks are connected in parallel on the air supply main pipe, the air pressure of the carbon dioxide air storage tanks is insufficient for automatic switching, the air supply main pipe is connected with the air inlet valve, a flow meter and a pressure reducing valve are installed on the air inlet main pipe, an environment monitoring sensor is installed in the carbon dioxide incubator, and the flow meter and the environment monitoring sensor are both electrically connected with the controller.
2. The butt-joint type carbon dioxide culture system as claimed in claim 1, wherein the isolator is provided with a return air channel, the return air main pipe is communicated with the return air channel, and the return air main pipe is provided with a return air valve.
3. The system of claim 1, wherein a stirring fan is installed in the carbon dioxide incubator.
4. The butt-joint type carbon dioxide cultivation system as claimed in claim 1, wherein the carbon dioxide cultivation box is communicated with a pressure compensation pipe, the pressure compensation pipe is communicated with the return air main pipe, the pressure compensation pipe is provided with a pressure compensation degerming filter and a pressure compensation pipeline valve, and a pressure compensation switch valve is connected between the pressure compensation degerming filter and the pressure compensation pipeline valve in parallel.
5. The system for culturing carbon dioxide as claimed in claim 4, wherein the air inlet valve, the air outlet valve, the pressure compensation pipeline valve and the pressure compensation switch valve are all solenoid valves and are electrically connected to the controller.
6. The butt joint type carbon dioxide culturing system as claimed in any one of claims 1 to 5, wherein a branch ventilation pipe is connected between the carbon dioxide storage tank and the main gas supply pipe, a pressure sensor and an electric gas inlet valve are mounted on the branch ventilation pipe, and the pressure sensor and the electric gas inlet valve are electrically connected with the controller.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921759830.7U CN211367585U (en) | 2019-10-18 | 2019-10-18 | Butt-joint carbon dioxide culture system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921759830.7U CN211367585U (en) | 2019-10-18 | 2019-10-18 | Butt-joint carbon dioxide culture system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211367585U true CN211367585U (en) | 2020-08-28 |
Family
ID=72152810
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921759830.7U Active CN211367585U (en) | 2019-10-18 | 2019-10-18 | Butt-joint carbon dioxide culture system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211367585U (en) |
-
2019
- 2019-10-18 CN CN201921759830.7U patent/CN211367585U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN212025366U (en) | Cell tissue culture device capable of accurately controlling gas pressure and concentration | |
| WO2019206207A1 (en) | Active ventilation assembly, active-ventilation-type bioreactor and cell culture device | |
| CN113755331B (en) | Cell incubator of simulation altitude environment | |
| CN211367585U (en) | Butt-joint carbon dioxide culture system | |
| CN208933375U (en) | Multifunctional microbial detection device | |
| CN218951379U (en) | Indoor proliferation culture system of haematococcus pluvialis | |
| CN104109631A (en) | Microalgae culture reactor | |
| CN215414407U (en) | Sterilization effect inspection auxiliary device of continuous type active toxicity effluent disposal system | |
| CN211814437U (en) | Culture box | |
| CN111205975B (en) | Carbon dioxide culture system | |
| CN208748101U (en) | Installation for fermenting and fermentation system | |
| CN211367554U (en) | Carbon dioxide incubator air supply pipeline normal position sterilization apparatus | |
| CN208933383U (en) | A kind of intelligent automation cell colony experimental facilities | |
| CN208844092U (en) | A kind of play a reversed role control system and its bioreactor based on bioreactor | |
| CN207468647U (en) | A kind of isolated carbon dioxide incubator of comparison | |
| CN204529875U (en) | The automatic culturing and adding device of a kind of microbial bacteria | |
| CN101543634B (en) | Method for sterilizing air bacteria filter for fermentation tank and fermentation tank provided with air bacteria filter | |
| CN215887052U (en) | Carbon dioxide incubator air flow system | |
| US11814614B2 (en) | Cell culture device | |
| CN217064834U (en) | Artificial phellinus igniarius spore liquid submerged culture device based on high altitude area | |
| CN210943017U (en) | Aseptic turnover case of microorganism sample | |
| CN213113332U (en) | Automatic add fermenting installation of defoaming agent | |
| CN216473229U (en) | Carbon dioxide incubator for cell culture | |
| CN110423690B (en) | Cell factory pipeline system | |
| CN211620532U (en) | CO with good sealing performance2Culture box |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |