CN217064834U - Artificial phellinus igniarius spore liquid submerged culture device based on high altitude area - Google Patents

Artificial phellinus igniarius spore liquid submerged culture device based on high altitude area Download PDF

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CN217064834U
CN217064834U CN202122795626.4U CN202122795626U CN217064834U CN 217064834 U CN217064834 U CN 217064834U CN 202122795626 U CN202122795626 U CN 202122795626U CN 217064834 U CN217064834 U CN 217064834U
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incubator
phellinus igniarius
culture device
altitude area
spore
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李傲然
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Lhasa Bomara Biotechnology Co ltd
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Lhasa Bomara Biotechnology Co ltd
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Abstract

The utility model discloses an artificial phellinus igniarius spore liquid submerged culture device based on high altitude areas, which comprises an incubator, a temperature controller and a humidifying box, wherein the bottom wall of the incubator is provided with a storage seat, a culture bottle is placed inside the storage seat, and the inner surface of the culture bottle is provided with the temperature controller; the bottom wall of the incubator is provided with a refrigerator, the inner walls of two sides of the incubator are provided with heaters, and the heaters and the refrigerator are electrically connected with the temperature controller; and a humidifying box is arranged on the outer wall of one side of the incubator. The utility model discloses an adjustment control to the temperature in the incubator, and then can make the phellinus igniarius spore in the blake bottle have different suitable temperatures in the growth period of difference for the absorption of phellinus igniarius spore to the nutrient, thereby improve cultivation efficiency, shorten culture time.

Description

Artificial phellinus igniarius spore liquid submerged culture device based on high altitude area
Technical Field
The utility model relates to a bacterial culture technical field specifically is a liquid deep culture device of artifical phellinus igniarius spore based on high altitude area.
Background
Phellinus igniarius has high medicinal value, but the number of natural Phellinus igniarius in China is very rare, the artificial cultivation of fruiting bodies is still at the beginning stage at present, if a stable medical industry product source is formed, population genetic diversity analysis is required, natural resources of Phellinus igniarius are sufficiently mined, in addition, strain separation culture is required, deep fermentation is carried out by using modern biotechnology, but the influence of temperature on Phellinus igniarius spores is not considered in the culture process of the existing culture device, and the culture efficiency is low.
The existing liquid submerged culture device has the following defects:
1. the utility model discloses a chinese utility model patent that publication number is CN205902434U discloses a tubular deep culture device, including multiunit fermentation unit, electromagnetic pump, ball formula filter, multiunit fermentation unit comprises at least a set of fermentation unit, and a set of fermentation unit includes fermentation cylinder, first three-way valve, and a play liquid end of first three-way valve is connected with the fermentation cylinder, and another play liquid end of first three-way valve is connected with a set of fermentation unit down, and the electromagnetic pump passes through the connecting pipe to be connected with the ball formula filter, and the feed liquor end of ball formula filter and first three-way valve is connected. The utility model discloses simple structure, the operation of being convenient for, whole operation process is airtight continuous, and the pollution is introduced to the nothing, realizes aseptic technique.
However, the submerged culture apparatus of the above-mentioned publication only considers the problem of the tightness during the operation, and does not consider the influence of the temperature on the artificial phellinus linteus spores during the culture process, especially in high altitude areas, where the air contains less oxygen, and is hot in summer and cold in winter, which is not good for the growth of phellinus linteus, and the growth cycle of the spores is easily influenced by unfavorable environmental conditions.
In view of the above, there is a need to develop a liquid submerged culture apparatus capable of regulating and controlling temperature, so as to avoid the influence of temperature variation in high altitude areas on the culture of phellinus igniarius spores, and improve the production efficiency of spores by regulating and controlling temperature.
2. In the process of culturing artificial phellinus igniarius spores by using the conventional liquid submerged culture device, the growth of the phellinus igniarius spores is greatly influenced by humidity factors, and the quality of the phellinus igniarius spores is prolonged in an over-wet or over-dry environment.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an artifical phellinus igniarius spore liquid submerged culture device based on high-altitude area to solve the problem that current artifical phellinus igniarius spore degree of depth culture device who mentions in the above-mentioned background art can not carry out control to the cultivation temperature.
In order to achieve the above object, the utility model provides a following technical scheme: an artificial phellinus igniarius spore liquid submerged culture device based on a high-altitude area comprises an incubator, a temperature controller and a humidifying box, wherein an object placing seat is installed on the bottom wall of the incubator, a culture bottle is placed inside the object placing seat, and the temperature controller is installed on the inner surface of the culture bottle;
the bottom wall of the incubator is provided with a refrigerator, the refrigerator is positioned on one side of the culture bottle, the inner walls of two sides of the incubator are provided with heaters, and the heaters, the refrigerator and the temperature controller are electrically connected;
and a humidifying box is arranged on the outer wall of one side of the incubator.
Preferably, control panel is installed in the front of humidification case, the suction pump is installed at the top of humidification case, the water delivery hose is installed to the output of suction pump, the surface mounting of water delivery hose has pressure boost shower nozzle, and pressure boost shower nozzle is located the inside of incubator, the dehumidifier is installed to one side inner wall of incubator, the outlet pipe is installed to the bottom of dehumidifier, and the one end of outlet pipe extends the outside of incubator, symmetrical arrangement's humidity transducer is installed to one side outer wall of dehumidifier.
Preferably, the gas holder is installed to the opposite side outer wall of incubator, the gas-supply pipe is installed on the top of gas holder, and the one end of gas-supply pipe extends into the inside of blake bottle, the surface mounting of gas-supply pipe has pressure valve, and pressure valve is located the outside of incubator.
Preferably, the liquid reserve tank is installed to the opposite side outer wall of incubator, and the liquid reserve tank is located the rear of gas holder, the water pump is installed at the top of liquid reserve tank, the transfer line is installed to the output of water pump, and the one end of transfer line extends into the inside of blake bottle, the surface mounting of transfer line has the choke valve, and the choke valve is located the outside of incubator.
Preferably, the front of incubator is provided with the notch, the front of incubator is installed through the hinge and is observed the door, and observes door and notch phase-match, the inside of observing the door is run through and is installed the glass board.
Preferably, the back surface of the observation door is provided with a sealing strip, and the outer surface of the sealing strip is attached to the inner surface of the notch.
Preferably, the top of the incubator is provided with ventilation pipes which are uniformly distributed in a penetrating manner, and one-way valves are arranged in the ventilation pipes.
Compared with the prior art, the beneficial effects of the utility model are that:
1. the utility model discloses an install temperature controller, refrigerator and heater, when temperature controller in the blake bottle detects that the temperature in the blake bottle rises to certain threshold value and is not conform to the spore growth, the refrigerator is opened, cool down the processing in the incubator, and when temperature controller should the temperature cross low excessively, can open the heater, play the effect that heaies up, ensure that the phellinus igniarius spore is under the suitable temperature condition all the time, thereby can make the absorption of phellinus igniarius spore to the nutrient more swift to a certain extent, thereby shorten the culture time.
2. The utility model discloses an install the humidification case, the suction pump, dehumidifier and humidity transducer, when humidity transducer on the dehumidifier detects the humidity index in the incubator and rises to certain threshold value, the dehumidifier is opened, take out except that the case through the unnecessary moisture in outlet pipe with the incubator, vice versa, when too dry when being unfavorable for spore growth in the incubator, add appropriate amount water in the humidification incasement, then start the suction pump, spray the water in the humidification incasement in the effect of water delivery hose in the incubator through pressure boost nozzle, and then can carry out the humidification to the incubator, ensure that the phellinus spore in the blake bottle is in under the appropriate environmental condition, thereby improve the culture rate of phellinus spore.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
fig. 2 is a plan view of the present invention;
fig. 3 is a schematic view of an assembly structure of the humidification case and the water pump of the present invention;
FIG. 4 is a schematic view of the assembly structure of the incubator and the culture bottle of the present invention;
FIG. 5 is a schematic view of the assembly structure of the incubator, the temperature controller and the ventilation tube according to the present invention.
In the figure: 1. an incubator; 2. a gas storage tank; 3. a liquid storage tank; 301. a water pump; 302. a throttle valve; 4. a culture bottle; 5. a temperature controller; 501. a refrigerator; 502. a heater; 6. a humidifying box; 601. a water pump; 602. a dehumidifier; 603. a humidity sensor; 7. and a vent pipe.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In a first embodiment, please refer to fig. 2 and fig. 4, the present invention provides an embodiment: the utility model provides an artificial phellinus igniarius spore liquid submerged culture device based on high-altitude area, includes blake bottle 4 and temperature controller 5, and the diapire of incubator 1 is installed and is put the thing seat, and the inside of putting the thing seat has placed blake bottle 4, and the internally mounted of blake bottle 4 has temperature controller 5, and refrigerator 501 is installed to the diapire of incubator 1, and refrigerator 501 is located one side of blake bottle 4, and heater 502 is all installed to the both sides inner wall of incubator 1, electric connection between heater 502 and refrigerator 501 and the temperature controller 5.
Specifically, when temperature controller 5 detects the temperature in blake bottle 4 too high, refrigerator 501 starts, and then can carry out the operation of cooling down to incubator 1 under refrigerator 501's effect, otherwise, when temperature controller 5 detects the temperature in blake bottle 4 and crosses lowly, heater 502 opens, and then can heat up the temperature in blake box 1 through heater 502, until temperature controller 5 detects the suitable phellinus igniarius spore growth of temperature of blake bottle 4, thereby can be through the adjustment control to the temperature, make the phellinus igniarius spore have different temperatures in different growth stages, not only can improve cultivation efficiency, and can shorten culture time.
In the second embodiment, as shown in fig. 3 and 5, an artificial phellinus igniarius spore liquid submerged culture device based on a high-altitude area comprises a humidifying box 6 and a ventilation pipe 7, the humidifying box 6 is installed on the outer wall of one side of an incubator 1, a control panel is installed on the front surface of the humidifying box 6, a water suction pump 601 is installed at the top of the humidifying box 6, a water delivery hose is installed at the output end of the water suction pump 601, a pressurizing nozzle is installed on the surface of the water delivery hose and located inside the incubator 1, a dehumidifier 602 is installed on the inner wall of one side of the incubator 1, a water outlet pipe is installed at the bottom of the dehumidifier 602, one end of the water outlet pipe extends out of the incubator 1, symmetrically-arranged humidity sensors 603 are installed on the outer wall of one side of the dehumidifier 602, the ventilation pipe 7 uniformly-arranged on the top of the incubator 1 in a penetrating manner, and a one-way valve is installed inside the ventilation pipe 7.
Specifically, add the water of capacity in humidification case 6 in advance, when too dry in incubator 1, start suction pump 601, in the water pumping hose that will humidify in case 6 under suction pump 601's effect, then spray in incubator 1 through pressure boost shower nozzle, and then can carry out the humidification to incubator 1 through the water smoke that sprays out, and when humidity transducer 603 detected the humidity index in incubator 1 when too high, dehumidifier 602 starts, thereby get rid of the moisture in incubator 1, built a suitable phellinus spore growth's humidity environment to a certain extent, thereby improve cultivated efficiency.
As shown in the attached figure 1, the artificial phellinus igniarius spore liquid submerged culture device based on the high altitude area comprises an incubator 1, an air storage tank 2 and a liquid storage tank 3, wherein a notch is formed in the front surface of the incubator 1, an observation door is installed on the front surface of the incubator 1 through a hinge and matched with the notch, a glass plate is installed inside the observation door in a penetrating manner, a sealing strip is installed on the back surface of the observation door, the outer surface of the sealing strip is attached to the inner surface of the notch, the air storage tank 2 is installed on the outer wall of the other side of the incubator 1, an air delivery pipe is installed at the top end of the air storage tank 2, one end of the air delivery pipe extends into the interior of a culture bottle 4, a pressure valve is installed on the outer surface of the air delivery pipe and is located outside the incubator 1, the liquid storage tank 3 is installed on the outer wall of the other side of the incubator 1, the liquid storage tank 3 is located behind the air storage tank 2, and a water pump 301 is installed at the top of the liquid storage tank 3, the transfer line is installed to the output of water pump 301, and the one end of transfer line extends into the inside of blake bottle 4, and the surface mounting of transfer line has choke valve 302, and choke valve 302 is located the outside of incubator 1.
It is specific, at first put into blake bottle 4 with the phellinus igniarius spore, then put into blake box 1 with blake bottle 4, then start water pump 301, can be with in the blake bottle 4 is carried into to the nutrient solution of mixing in the liquid reserve tank 3 through the transfer line, under the effect of choke valve 302, make the nutrient solution in the liquid reserve tank 3 can be quantitative for the phellinus igniarius spore in the blake bottle 4 provides the required nutrient substance of growth, open the pressure valve, the oxygen mixture in the gas holder 2 can be carried into blake bottle 4 through the gas-supply pipe under the effect of pressure in, and the produced waste gas of phellinus igniarius spore growth breathing can float upwards under the oxygen mixture impact, then distribute away through ventilation pipe 7, thereby gas circulation in the blake box 1 has been accelerated.
The working principle is as follows: after the phellinus igniarius spores are placed in the culture bottle 4, the culture bottle 4 is placed on the storage seat, the observation door is closed, then the uniformly mixed nutrient solution is added into the liquid storage tank 3, the water pump 301 is started, the nutrient solution in the liquid storage tank 3 can be conveyed into the culture bottle 4 through the liquid conveying pipe, so that nutrition required by growth can be provided for the phellinus igniarius spores in the culture bottle 4, in the process, the pressure valve can also be opened, the oxygen mixture in the gas storage tank 2 can be conveyed into the culture bottle 4, and the sufficient oxygen mixture can be provided for the spores;
in the culture process, when the temperature controller 5 in the culture bottle 4 detects that the temperature in the culture bottle 4 is too high and is not beneficial to the growth of phellinus igniarius spores, the refrigerator 501 is started to cool the interior of the culture box 1, on the contrary, when the temperature is too low, the heater 502 is started, and the temperature in the culture box 1 is increased through the heater 502 until the phellinus igniarius spores are suitable to grow;
when the humidity sensor 603 on the dehumidifier 602 detects that the humidity index in the incubator 1 is too high, the dehumidifier 602 is started, moisture in the incubator 1 is discharged out of the incubator under the action of the dehumidifier 602, on the contrary, when the incubator 1 is too dry, a worker can place enough water in the humidification box 6 in advance, then the water pump 601 is started, and the water in the humidification box 6 can be sprayed into the incubator 1 through the pressurizing nozzle under the action of the water delivery hose, so that the humidification purpose is achieved.
The above-described embodiments of the apparatus are merely illustrative, and the units described as separate parts may or may not be physically separate, and parts displayed as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of the present embodiment. One of ordinary skill in the art can understand and implement it without inventive effort.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims (7)

1. The utility model provides an artificial phellinus igniarius spore liquid submerged culture device based on high-altitude area, includes incubator (1), temperature controller (5) and humidification case (6), its characterized in that: the bottom wall of the incubator (1) is provided with a storage seat, a culture bottle (4) is placed in the storage seat, and the inner surface of the culture bottle (4) is provided with a temperature controller (5);
a refrigerator (501) is installed on the bottom wall of the incubator (1), the refrigerator (501) is located on one side of the culture bottle (4), heaters (502) are installed on the inner walls of the two sides of the incubator (1), and the heaters (502) and the refrigerator (501) are electrically connected with the temperature controller (5);
the outer wall of one side of the incubator (1) is provided with a humidifying box (6).
2. The high-altitude area-based artificial phellinus igniarius spore liquid submerged culture device according to claim 1, wherein: control panel is installed in the front of humidification case (6), suction pump (601) are installed at the top of humidification case (6), water delivery hose is installed to the output of suction pump (601), water delivery hose's surface mounting has the pressure boost shower nozzle, and the pressure boost shower nozzle is located the inside of incubator (1), dehumidifier (602) are installed to one side inner wall of incubator (1), the outlet pipe is installed to the bottom of dehumidifier (602), and the one end of outlet pipe extends the outside of incubator (1), symmetrical arrangement's humidity transducer (603) is installed to one side outer wall of dehumidifier (602).
3. The high-altitude area-based artificial phellinus igniarius spore liquid submerged culture device according to claim 1, wherein: air holder (2) are installed to the opposite side outer wall of incubator (1), the gas-supply pipe is installed on the top of gas holder (2), and the one end of gas-supply pipe extends into the inside of blake bottle (4), the surface mounting of gas-supply pipe has pressure valve, and pressure valve is located the outside of incubator (1).
4. The high-altitude area-based artificial phellinus igniarius spore liquid submerged culture device according to claim 1, wherein: liquid reserve tank (3) are installed to the opposite side outer wall of incubator (1), and liquid reserve tank (3) are located the rear of gas holder (2), water pump (301) are installed at the top of liquid reserve tank (3), the transfer line is installed to the output of water pump (301), and the one end of transfer line extends into the inside of blake bottle (4), the surface mounting of transfer line has choke valve (302), and choke valve (302) are located the outside of incubator (1).
5. The high-altitude area-based artificial phellinus igniarius spore liquid submerged culture device according to claim 1, wherein: the front of incubator (1) is provided with the notch, the front of incubator (1) is installed through the hinge and is observed the door, and observes door and notch phase-match, the inside of observing the door is run through and is installed the glass board.
6. The high-altitude area-based artificial phellinus igniarius spore liquid submerged culture device according to claim 5, wherein: the back surface of the observation door is provided with a sealing strip, and the outer surface of the sealing strip is attached to the inner surface of the groove opening.
7. The high-altitude area-based artificial phellinus igniarius spore liquid submerged culture device according to claim 1, wherein: the top of incubator (1) is run through and is installed ventilation pipe (7) of evenly arranging, the internally mounted of ventilation pipe (7) has the check valve.
CN202122795626.4U 2021-11-16 2021-11-16 Artificial phellinus igniarius spore liquid submerged culture device based on high altitude area Active CN217064834U (en)

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CN202122795626.4U CN217064834U (en) 2021-11-16 2021-11-16 Artificial phellinus igniarius spore liquid submerged culture device based on high altitude area

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CN202122795626.4U CN217064834U (en) 2021-11-16 2021-11-16 Artificial phellinus igniarius spore liquid submerged culture device based on high altitude area

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CN217064834U true CN217064834U (en) 2022-07-29

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