CN217922091U - Controllable microorganism incubator of three rooms of constant temperature and humidity - Google Patents

Controllable microorganism incubator of three rooms of constant temperature and humidity Download PDF

Info

Publication number
CN217922091U
CN217922091U CN202222071199.XU CN202222071199U CN217922091U CN 217922091 U CN217922091 U CN 217922091U CN 202222071199 U CN202222071199 U CN 202222071199U CN 217922091 U CN217922091 U CN 217922091U
Authority
CN
China
Prior art keywords
box body
air
culture chamber
culture
humidity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202222071199.XU
Other languages
Chinese (zh)
Inventor
王慧丽
张晓可
郭文轩
柳颖
许雅雯
宋梦怡
宋晓贺
胡梅月
姚军
马力
袁学娟
崔玉敏
王晓
常杰伦
李静
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hebei Xinteng Biotechnology Co.,Ltd.
Original Assignee
Anqing Normal University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anqing Normal University filed Critical Anqing Normal University
Priority to CN202222071199.XU priority Critical patent/CN217922091U/en
Application granted granted Critical
Publication of CN217922091U publication Critical patent/CN217922091U/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25Greenhouse technology, e.g. cooling systems therefor

Abstract

The utility model discloses a constant temperature and humidity three-chamber controllable microorganism incubator, wherein the top of a box body is provided with an ultraviolet sterilizing lamp, a PTC (positive temperature coefficient) ceramic heating pipe and an air inlet ventilation device, the bottom of an air delivery pipe is communicated with a plurality of air outlet pipes which are the same in size and are uniformly distributed, a high-energy bubble washing and drying device is arranged below the air inlet ventilation device, two sides of the top of the box body are provided with centrifugal atomizing fans, aerobic, facultative and anaerobic culture chambers are arranged in the box body from top to bottom, a heat insulation board is arranged below the bottom plate of each culture chamber, and each culture chamber is provided with a plurality of infrared monitoring cameras and multispectral plant LED growth lamp tubes and temperature, humidity and oxygen sensors; a refrigeration compressor, a central processing unit, an oxygen increasing and pumping device, a water adding and humidifying device, a vacuum pump, an air source tank, an air pressure sensor and a water outlet are arranged below the shaking table. Emphasizes that different microorganisms grow in respective optimal culture environments, and has the characteristics of bidirectional temperature regulation of refrigeration and heating, convenience for observation, cleaning and disinfection.

Description

Controllable microorganism incubator of three rooms of constant temperature and humidity
Technical Field
The utility model relates to a microbial cultivation case especially relates to a controllable microbial cultivation of three rooms of constant temperature and humidity.
Background
The microbial cultivation refers to the cultivation conditions artificially created by means of a culture medium which is artificially prepared, so that certain microbes can quickly grow and propagate, and the microbial cultivation is required by scientific research departments such as biology, agriculture, medicine, environmental protection and the like. The growth state of microorganisms needs to be observed and recorded at regular time in the process of culturing the microorganisms, the state of the microorganisms cannot be observed visually in the conventional incubator, and a culture vessel needs to be taken out of the incubator for observation, so that the possibility of contamination to the microorganisms is brought to the culture of the microorganisms, and the reliability of final detection data is influenced; on the other hand, the difference between the culture environment (including temperature, humidity, etc.) inside the incubator and the environment outside the incubator is large, and the relatively stable temperature and humidity environment inside the incubator is damaged by the action of opening the incubator to take out the culture vessel, thereby affecting the growth of the microorganisms. Microorganisms of various growth conditions grow in the same culture environment, resulting in poor growth of the cultured microorganisms.
SUMMERY OF THE UTILITY MODEL
Not enough to current technique, the utility model provides a three controllable microorganisms of constant temperature and humidity cultivate, solved illumination, temperature, humidity, the oxygen inhomogeneity that the microorganism cultivation in-process appears, and culture apparatus is not convenient for observe, cleaning and disinfecting's problem, the temperature in each cultivation region is avoided in three room cultivations influences each other, avoids the observation action in the cultivation process to produce the pollution to the cultivation environment of microorganism simultaneously, also avoids producing destruction scheduling problem to the relatively stable cultivation environment in the box.
In order to solve the technical problem, the utility model discloses a following technical scheme: a constant-temperature and constant-humidity three-chamber controllable microorganism incubator comprises a box body with pulleys at the bottom, wherein an ultraviolet sterilization lamp, a PTC (positive temperature coefficient) ceramic heating pipe and an air inlet ventilation device are installed at the top of the box body, an output port of the air inlet ventilation device is provided with active carbon and a filter membrane and is connected with an air conveying pipe, the bottom of the air conveying pipe is communicated with a plurality of air outlet pipes which are same in size and are uniformly distributed, a high-energy bubble washing and drying device is installed below the air inlet ventilation device, centrifugal atomizing fans are installed on two sides of the top of the box body, an aerobic culture chamber, a facultative culture chamber and an anaerobic culture chamber are arranged in the box body from top to bottom, a bottom plate of each culture chamber is inserted into sliding grooves in two sides of the box body, a culture dish clamping groove is formed in the bottom plate, a heat insulation plate is arranged below the bottom plate, the middle of the heat insulation plate is hollow, a plurality of through holes communicated with the hollow are formed in the surface of the heat insulation plate, and the through holes are communicated with the indoor space; the top of each culture chamber is provided with a plurality of infrared monitoring cameras and multispectral plant LED growth lamp tubes, and the side surface of each culture chamber is provided with a temperature sensor, a humidity sensor and an oxygen sensor; the anaerobic culture chamber below be equipped with the shaking table, the below installation refrigeration compressor, central processing unit, oxygenation oxygen pumping device, add water humidification device, vacuum pump, air supply jar, baroceptor, outlet of shaking table, the chamber door of box be equipped with the display screen, its below is equipped with the alarm.
Compared with the prior art, the utility model provides a possess controllable microorganism incubator of three rooms of constant temperature and humidity sets up three controllable regulations according to different cultivation conditions, including good oxygen cultivation room, facultative anaerobic cultivation room, anaerobism cultivation room, each cultivates relative closed and sets up independent temperature control system in the region, can set up different conditions such as temperature, humidity, oxygen, illumination according to the microorganism demand in each cultivation region. The problems that the temperature in each culture area influences each other, the observation action in the culture process pollutes the culture environment of microorganisms, the relatively stable culture environment in the box body is damaged and the like are solved, the emphasis is placed on enabling different microorganisms to grow in the respective optimal culture environment, and the box has the characteristics of bidirectional temperature regulation of refrigeration and heating, convenience in observation, cleaning and disinfection.
Drawings
FIG. 1 is a schematic structural view of the three-chamber constant temperature and humidity controllable microorganism incubator of the utility model.
Detailed Description
As shown in fig. 1, the utility model discloses a constant temperature and humidity three-chamber controllable microorganism incubator, including the box 2 that the footing has pulley 1, the top of box 2 install ultraviolet sterilizing lamp 3, PTC ceramic heating pipe 4 and air inlet ventilation unit 5, the delivery outlet of air inlet ventilation unit 5 be equipped with active carbon and filter membrane and with defeated tuber pipe connection, defeated tuber pipe's bottom intercommunication has a plurality of play tuber pipes that the size is the same and distribute evenly, install high energy bubble below the air inlet ventilation unit 5 and wash and drying device 6, box 2's top both sides installation centrifugal atomizing fan 7, box 2 is interior to be equipped with aerobic culture room 8, facultative culture room 9 and anaerobic culture room 10 from top to bottom, the spout 12 of each culture room's bottom plate 11 insertion box 2 both sides, bottom plate 10 can be pulled, be equipped with the culture dish draw-in groove on the bottom plate 11, bottom plate 11 below installation heat insulating board 13, 13 middle cavity, open on the surface of heat insulating board 13 has a lot of through-hole with cavity intercommunication, the through-hole communicates with indoor with; the top of each culture chamber is provided with a plurality of infrared monitoring cameras 14 and a multispectral plant LED growth lamp tube 28, the infrared monitoring cameras and a hollow toughened glass door convex lens form a magnifier to more conveniently observe indoor conditions, and the side surface of each culture chamber is provided with a temperature sensor 15, a humidity sensor 16 and an oxygen sensor 17; a shaking table 18 is arranged below the anaerobic culture chamber 10, a refrigeration compressor 19, a central processing unit 20, an oxygen increasing and pumping device 21, a water adding and humidifying device 22, a vacuum pump 23, an air source tank 24, an air pressure sensor 25, a water outlet and the like are arranged below the shaking table 18, a display screen 26 is arranged on a box door of the box body 2 and can monitor the growth condition of microorganisms, and an alarm 27 is arranged below the box body.
The output electric connection of temperature, humidity, oxygen sensor is in data transmission module's input, data transmission module's output electric connection is in data analysis module's input, data analysis module's output electric connection is in central processing unit's input, central processing unit's output electric connection respectively in display and control module's input, control module's output electric connection respectively in PTC ceramic heating pipe, centrifugal spray fan, compressor and the input of ventilation unit that admits air.
The output end of the infrared monitoring camera is electrically connected to the input end of the data transmission module, the output end of the data transmission module is electrically connected to the input end of the data analysis module, the output end of the data analysis module is electrically connected to the input end of the central processing unit, and the output end of the central processing unit is electrically connected to the input end of the display. A shaking table is arranged under the microorganism incubator, so that the oxygen requirement in the microorganism growth process is fully met.
The utility model discloses operating method includes following step: the required microorganism is placed in a culture dish, and the temperature and the humidity in the culture box are adjusted to the optimal temperature required by the growth of the microorganism through a temperature sensing probe, a humidity sensing probe, an infrared monitoring camera and the like. Meanwhile, an oxygen increasing and pumping device is used for maintaining the oxygen in the incubator in an optimal range. And after the temperature and the humidity reach certain standards, an alarm can be sent to an experimenter through the alarm to adjust. The problem of unstable temperature of the constant temperature incubator can be effectively solved according to the heating pipe and the air heating control system. The humidifying tube has the effects of centrifugal spraying and breeze airflow circulation, and can effectively maintain the humidity in the incubator. Can effectively avoid the rapid evaporation of water due to overhigh temperature in the incubator, which is not beneficial to the growth of microorganisms.
The system is suitable for anaerobic, facultative and aerobic culture of microorganisms, in order to enable the oxygen concentration in a culture tank to be 0% in an anaerobic chamber, the anaerobic chamber can be continuously executed twice according to the air extraction and inflation mode of microaerobic culture, a controller is started, an electromagnetic valve is opened by the controller, a vacuum pump is started to vacuumize the culture chamber, an air pressure sensor transmits the air pressure in the culture chamber to the controller, when the vacuum degree in the culture tank reaches a set vacuum degree, the controller closes the vacuum pump and the electromagnetic valve, a high-pressure air source starts to fill mixed gas into the culture chamber, 90% nitrogen, 5% hydrogen and 5% carbon dioxide are filled, and when air needs to be exhausted, a pressure reducing valve can be opened to exhaust the air; the aerobic culture chamber is supplemented with oxygen from 2% to 20% according to different oxygen demands.
Compared with the prior art, the utility model provides a possess controllable microorganism incubator of three rooms of constant temperature and humidity, possess following beneficial effect:
1. the utility model discloses set up three controllable regulations of room according to different cultivation conditions, including good oxygen cultivation room, facultative anaerobic cultivation room, anaerobism cultivation room, each cultivates the relative seal in the region and sets up independent temperature control system, can set up different conditions such as temperature, humidity, oxygen, illumination according to the microorganism demand in each cultivation region.
2. The utility model discloses six direct heating designs of PTC ceramic heating pipe air-heating control system have been set up, provide gentle thermal convection circulation in the box to realize outstanding thermostatic control. PID microcomputer intelligent temperature controller and infrared inductor, thermosensitive element as thermometer, digital display of set value and measured value, precise monitoring and control, and creation of high-stability culture environment for microbe growth. The heating system can be heated to 60 ℃ when the ambient temperature is-5 ℃, and the ultraviolet sterilizing lamp is used for sterilizing, so that bacterial pollution is effectively prevented.
3. Humidification pipe, centrifugal spray fan, compressor, air inlet ventilation unit, temperature sensing wet probe, temperature and humidity sensor, central processing unit, data transmission module, data analysis module, control module have solved current microorganism incubator and do not possess the function of constant temperature and humidity when using, can't ensure the growth environment of microorganism to the problem that the people of not being convenient for used. The air heating control system is used for solving the problem of unstable temperature of the constant temperature box; the humidifying pipe humidifies the whole incubator by adopting centrifugal spraying and breeze airflow circulation, so that the humidity in the incubator can be effectively maintained, and the situation that water is rapidly evaporated due to overhigh temperature in the incubator and is not beneficial to the growth of microorganisms can be effectively avoided.
4. The utility model discloses a but set up the pull track, but be equipped with the culture dish draw-in groove on the pull track. The convenience is placed the culture dish, makes things convenient for the operation personnel to look over the microorganism, is particularly suitable for making the biological sample's of multiple contrast test cultivation equipment through setting up ring groove and omnipotent spring test bottle frame, effectively improves the stability of culture dish at the pull in-process.
5. The utility model discloses a set up infrared surveillance camera head in culture dish draw-in groove top, cavity toughened glass door convex lens form the magnifying glass and can make things convenient for the observation room condition more, and infrared surveillance camera head is connected with the chamber door display, the display screen is located culture apparatus openly for show the microorganism growth condition, can make the microorganism growth condition of watching that operating personnel can be clearer through the display, avoid frequent switch chamber door to appear the risk of dying fungus to the microorganism culture in-process on the one hand, on the other hand avoids box external environment to produce the influence to temperature, humidity of setting for in the box.
6. The utility model discloses a set up the shaking table in microorganism incubator below, make things convenient for the operation personnel to cultivate the microorganism, increase microorganism and oxygen contact, carry out remote transfer when avoiding the microorganism to cultivate, cause pollution or unrestrained. The eccentric shaft with five shafts integrated is adopted to drive the shaking table, and the protective cover completely isolates the whole driving structure from the culture cavity, so that the influence of the shaking table on the culture environment during operation is reduced to the minimum. The intelligent control circuit ensures that the shaking table is started slowly, is stable in acceleration and protects the safety of experimental samples. The shaking table can provide stable, uniform and vibration-free operating conditions for suspended microbe culture, adopts an advanced touch screen control panel, can clearly and accurately judge the growth state of microbes, and tracks the culture process and other characteristics and functions in real time.
7. Because the vacuum pump, the air source tank, the electromagnetic valve, the air pressure sensor and the controller are matched for regulation, the oxygen content in the culture chamber can be regulated through the air exchange times and the vacuum degree in the air source tank according to the requirements of the microbial environment, and the controller, the vacuum pump, the air source tank and the air pressure sensor are placed below the rear part of the box body.
8. The air inlet ventilation device is arranged, the air conveying pipe is fixedly connected with, the bottom of the air conveying pipe is communicated with a plurality of air outlet pipes with the same size and uniform distribution, so that dust and mixed bacteria in the air are prevented from entering the inner cavity of the microorganism incubator body, and particles with the diameter of more than 99.97 percent and more than 0.3 micrometer can be filtered and removed. The air inlet ventilation device is formed by four or five layers of activated carbon and a filter membrane repeatedly, and dust and other contained substances in air are filtered while ventilation is performed.
9. Multispectral plant LED growth lamp tubes are mounted on the side face and the top of each culture chamber, so that the light received by the culture box is more uniform and comprehensive, the growth of cultures is facilitated, and the disease resistance is improved.
10. The heat insulation board material of the box body is made of polyurethane foamed foam plastic, and has strong anti-interference capability to external heat (cold) sources. Because the cavity of the partition wall is communicated with the external environment, after the automatic microbial cultivation ventilation system is placed in the incubator, the cavity in the partition wall is communicated with the incubator through the through hole, so that heat exchange is promoted, and the left cavity and the right cavity in the tank body can quickly reach the required temperature; the inner layer is processed by adopting a high-quality stainless steel mirror panel, and the full stainless steel tray is rust-proof, bacteriostatic and convenient to clean.
11. High energy bubble is washed and drying device: the water spraying rocker arm nozzle is used for spraying water flow at more angles, water is sprayed from the center, then air is sucked through negative pressure and mixed with water, and water flow with water and bubbles is sprayed to form pulse type impact. The energy generated by the burst of the air bubbles is used for stripping the stains, and the cleaning capability is stronger than that of the common water flow.
12. The timing function is provided: the culture time is set arbitrarily within 0-999.9 hours, the power failure memory function is realized, the data loss caused by power failure and dead halt is avoided, the overtemperature acousto-optic alarm function and the abnormal condition automatic power failure function are realized, the fault self-diagnosis function is realized after the restart is realized, and the heating is automatically stopped when the actually measured temperature deviates from the set temperature and exceeds 3 ℃. And an audible alarm is given to ensure the safety of the experimental sample.

Claims (1)

1. A constant-temperature and constant-humidity three-chamber controllable microorganism incubator is characterized in that: the bottom of the box body (2) is provided with a box body (2) with pulleys (1), the top of the box body (2) is provided with an ultraviolet sterilizing lamp (3), a PTC ceramic heating pipe (4) and an air inlet ventilation device (5), an output port of the air inlet ventilation device (5) is provided with activated carbon and a filter membrane and is connected with an air delivery pipe, the bottom of the air delivery pipe is communicated with a plurality of air outlet pipes which are the same in size and are uniformly distributed, a high-energy bubble washing and drying device (6) is arranged below the air inlet ventilation device (5), centrifugal atomizing fans (7) are arranged on two sides of the top of the box body (2), an aerobic culture chamber (8), a facultative culture chamber (9) and an anaerobic culture chamber (10) are arranged in the box body (2) from top to bottom, a bottom plate (11) of each culture chamber is inserted into sliding chutes (12) on two sides of the box body (2), a culture dish clamping groove is arranged on the bottom plate (11), a heat insulation plate (13) is arranged below the bottom plate (11), the middle of the heat insulation plate (13) is hollow, a plurality of through holes communicated with the hollow space are arranged on the surface of the heat insulation plate (13); the top of each culture chamber is provided with a plurality of infrared monitoring cameras (14) and multispectral plant LED growth lamp tubes (28), and the side surface of each culture chamber is provided with a temperature sensor (15), a humidity sensor (16) and an oxygen sensor (17); the anaerobic culture chamber is characterized in that a shaking table (18) is arranged below the anaerobic culture chamber (10), a refrigeration compressor (19), a central processing unit (20), an oxygen increasing and pumping device (21), a water adding and humidifying device (22), a vacuum pump (23), an air source tank (24), an air pressure sensor (25) and a water outlet are arranged below the shaking table (18), a display screen (26) is arranged on a box door of the box body (2), and an alarm (27) is arranged below the box door.
CN202222071199.XU 2022-08-08 2022-08-08 Controllable microorganism incubator of three rooms of constant temperature and humidity Active CN217922091U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202222071199.XU CN217922091U (en) 2022-08-08 2022-08-08 Controllable microorganism incubator of three rooms of constant temperature and humidity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202222071199.XU CN217922091U (en) 2022-08-08 2022-08-08 Controllable microorganism incubator of three rooms of constant temperature and humidity

Publications (1)

Publication Number Publication Date
CN217922091U true CN217922091U (en) 2022-11-29

Family

ID=84152644

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202222071199.XU Active CN217922091U (en) 2022-08-08 2022-08-08 Controllable microorganism incubator of three rooms of constant temperature and humidity

Country Status (1)

Country Link
CN (1) CN217922091U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116590139A (en) * 2023-07-11 2023-08-15 一恒生命科学仪器(昆山)有限公司 Intelligent LED light-operated biological incubator and use method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116590139A (en) * 2023-07-11 2023-08-15 一恒生命科学仪器(昆山)有限公司 Intelligent LED light-operated biological incubator and use method thereof
CN116590139B (en) * 2023-07-11 2023-11-03 一恒生命科学仪器(昆山)有限公司 Intelligent LED light-operated biological incubator and use method thereof

Similar Documents

Publication Publication Date Title
CN207047254U (en) Cell culture incubator with warning device
CN217922091U (en) Controllable microorganism incubator of three rooms of constant temperature and humidity
CN112266869A (en) Laboratory is with little biological culture device
CN111334423A (en) Microbial incubator
CN203474812U (en) Microorganism culture apparatus
CN113755331B (en) Cell incubator of simulation altitude environment
CN111793561A (en) Carbon dioxide incubator
CN208917198U (en) A kind of microorganism fungus kind cultivation climate regulation equipment
CN209152044U (en) A kind of bat moth incubator
CN208898870U (en) A kind of clinical medicine medical treatment viable bacteria incubator
CN208167016U (en) A kind of microorganism incubator
CN207918856U (en) A kind of medical Incubators for bacteria culture
CN213662848U (en) Edible fungus culture device
CN214612458U (en) A culture apparatus for microbiological examination
CN113797368A (en) Two-in-one efficient sterilization incubator
CN109022265A (en) A kind of microbiological incubator
CN211999729U (en) Biochemical incubator is used in laboratory that intelligent degree is high
CN213680713U (en) Novel experimental research device for microalgae culture conditions
CN212357206U (en) Microbial cultivation case
CN218561428U (en) Microbial cultivation analytical equipment that humiture is even
CN208182984U (en) A kind of biology laboratory microbial cultivation device
CN208898887U (en) A kind of solid-state fermenter humidity conditioner preparing probiotics
CN212688077U (en) Biochemical incubator of on-line monitoring dust
CN217064834U (en) Artificial phellinus igniarius spore liquid submerged culture device based on high altitude area
CN215440461U (en) Be suitable for bacillus thuringiensis solid culture fermenting installation

Legal Events

Date Code Title Description
GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right

Effective date of registration: 20240223

Address after: Room 702, Unit 1, Building 6, Yi'an Jiayuan, No. 456 Yucai North Street, Taocheng District, Hengshui City, Hebei Province, 053000

Patentee after: Zhang Shaoguang

Country or region after: China

Address before: 246133 No. 1318 Jixian North Road, Anqing City, Anhui Province

Patentee before: ANQING NORMAL University

Country or region before: China

TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20240401

Address after: 339, 3rd Floor, Kanglong Building, No. 881, Building 1, North Side of Shengli West Road, Taocheng District, Hengshui City, Hebei Province, 053000

Patentee after: Hebei Xinteng Biotechnology Co.,Ltd.

Country or region after: China

Address before: Room 702, Unit 1, Building 6, Yi'an Jiayuan, No. 456 Yucai North Street, Taocheng District, Hengshui City, Hebei Province, 053000

Patentee before: Zhang Shaoguang

Country or region before: China