CN220587153U

CN220587153U - Easily observe plant tissue culture dish

Info

Publication number: CN220587153U
Application number: CN202322266818.5U
Authority: CN
Inventors: 崔刚; 徐波
Original assignee: Shandong Pulante Agricultural Technology Co ltd
Current assignee: Shandong Pulante Agricultural Technology Co ltd
Priority date: 2023-08-23
Filing date: 2023-08-23
Publication date: 2024-03-15
Anticipated expiration: 2033-08-23

Abstract

The utility model discloses an easily-observed plant tissue culture dish which comprises a dish cover and a dish body; the height of the dish cover is 8-15mm, a cylindrical air port is arranged in the center of the top of the dish cover, a cotton plug is arranged in the air port, 4 identical circular convex lenses which are vertically and equidistantly arranged are arranged on the top of the dish cover, an upper mark is arranged on the side wall of the dish cover, and the upper mark is positioned on the projection of a connecting line of the center of the dish cover and the center of the convex lens; the side wall of the dish body is provided with a lower mark, and the included angle between the lower mark and the central connecting line of the dish body is 90 degrees.

Description

Easily observe plant tissue culture dish

Technical Field

The utility model belongs to the field of plant tissue culture equipment, and particularly relates to an easily observable plant tissue culture dish.

Background

Tissue culture refers to a technique of separating desired tissues from plant bodies, placing the separated tissues into a culture dish, culturing under manual control by aseptic operation to obtain regenerated complete plants or producing other products with economic value, and along with the development of agriculture, tissue culture technique is also widely used in agriculture. At present, the culture dish can be used for plant materials, microorganism culture and adherent culture of animal cells, is suitable for operations of laboratory inoculation, streaking and bacteria separation, and can be used for plant material culture. However, when the existing culture dish is used, the growth of plant tissues is affected by poor ventilation condition, water mist is often formed in the dish cover due to water evaporation, so that the tissue culture observation is affected, meanwhile, the plant tissues are smaller in the initial stage of tissue culture due to the fact that the ordinary culture dish needs to be opened to be close to the cover for observing clearly, and the pollution risk is increased.

Disclosure of Invention

The utility model aims to solve the defects in the prior art, and provides an easily-observed plant tissue culture dish which has good ventilation effect, can be used for easily observing the growth condition of fine plant tissues through a convex lens, and reduces the pollution risk caused by uncovering observation.

In order to achieve the above purpose, the present utility model provides the following technical solutions: an easily-observed plant tissue culture dish comprises a dish cover and a dish body; the height of the dish cover is 8-15mm, a cylindrical air port is arranged in the center of the top of the dish cover, a cotton plug is arranged in the air port, 4 identical circular convex lenses which are vertically and equidistantly arranged are arranged on the top of the dish cover, an upper mark is arranged on the side wall of the dish cover, and the upper mark is positioned on the projection of a connecting line of the center of the dish cover and the center of the convex lens; the side wall of the dish body is provided with a lower mark, and the included angle between the lower mark and the central connecting line of the dish body is 90 degrees.

Preferably, the dish cover and the dish body are made of glass.

Preferably, the diameter of the gas port is 0.5-1cm.

Preferably, the cotton plug is made of defatted cotton by extrusion molding.

Preferably, the diameter of the dish body is 6-15cm.

Preferably, the diameter of the dish cover is 5-10mm larger than the diameter of the dish body.

Preferably, the upper mark is near the bottom of the side wall of the dish cover, and the lower mark is located 1-2mm below the dish cover when the dish cover is closed.

Compared with the prior art, the utility model has the beneficial effects that: firstly, the design is simple and reasonable, and the manufacturing is easy; secondly, the ventilation effect is good, the air required by the growth of plant tissues can be met, and the problems of fog formation on the inner wall of the culture dish, influence on observation and secondary pollution can be prevented; thirdly, through the convex lens, the growth condition of the tiny plant tissues is easier to observe, and the problem that the pollution caused by the close observation of the uncapping is avoided to influence the plant tissue culture is avoided.

Other features of the present disclosure and its advantages will become apparent from the following detailed description of exemplary embodiments of the disclosure, which proceeds with reference to the accompanying drawings.

Drawings

In order to more clearly illustrate the embodiments of the present disclosure or the technical solutions in the related art, the drawings that are required to be used in the embodiments or the related technical descriptions will be briefly described below, and it is apparent that the drawings in the following description are only embodiments of the present disclosure, and other drawings may be obtained from the provided drawings without inventive effort to those of ordinary skill in the art.

FIG. 1 is a schematic view of the entire culture dish according to the present utility model;

FIG. 2 is a partial schematic view of a culture dish according to the utility model.

In the figure: 1. the device comprises a dish cover 11, a cotton plug 12, an air port 13, a convex lens 14, an upper mark 2, a dish body 21 and a lower mark.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. The following description of at least one exemplary embodiment is merely exemplary in nature and is in no way intended to limit the utility model, its application, or uses. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

The relative arrangement of the components and steps, numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present utility model unless it is specifically stated otherwise. Meanwhile, it should be understood that the sizes of the respective parts shown in the drawings are not drawn in actual scale for convenience of description. Techniques, methods, and apparatus known to one of ordinary skill in the relevant art may not be discussed in detail, but should be considered part of the specification where appropriate. In all examples shown and discussed herein, any specific values should be construed as merely illustrative, and not a limitation. Thus, other examples of the exemplary embodiments may have different values. It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further discussion thereof is necessary in subsequent figures.

Spatially relative terms, such as "above … …," "above … …," "upper surface at … …," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial location relative to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations in use or operation in addition to the orientation depicted in the figures. For example, if the device in the figures is turned over, elements described as "above" or "over" other devices or structures would then be oriented "below" or "beneath" the other devices or structures. Thus, the exemplary term "above … …" may include both orientations of "above … …" and "below … …". The device may also be positioned in other different ways (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.

Referring to fig. 1 and 2, the present utility model provides a technical solution: an easily-observed plant tissue culture dish comprises a dish cover 1 and a dish body 2, wherein the dish cover 1 and the dish body 2 are made of glass; the height of the dish cover 1 is 10mm, a cylindrical air port 12 is arranged in the center of the top of the dish cover 1, the diameter of the air port 12 is 0.6cm, a cotton plug 11 is arranged in the air port 12, the cotton plug 11 is made of defatted cotton through extrusion molding, 4 identical circular convex lenses 13 which are vertically and equidistantly arranged are arranged on the top of the dish cover 1, an upper mark 14 is arranged on the side wall of the dish cover 1, and the upper mark 14 is positioned on the projection of a connecting line between the center of the dish cover 1 and the center of the convex lenses 13; the dish body 2 lateral wall is equipped with down mark 21, and dish body 2 diameter is 9cm, and dish lid 1 diameter is greater than dish body 2 diameter 8mm, down mark 21 and dish body 2 central line's contained angle be 90, and upper mark 14 is close to dish lid 1 lateral wall bottom, down mark 21 is located dish lid 1 below 2mm department when lid 1 is covered back to the dish.

When the culture dish is used, the cotton plug 11 is plugged into the air port 12, the air port 12 is covered and pinched by paper, the culture dish is subjected to high-temperature high-pressure steam sterilization, the culture dish is transferred into the ultra-clean workbench, after ultraviolet sterilization, the paper covered on the air port 12 is removed, the tissue culture medium is poured into the culture dish, and inoculated after solidification, so that the tissue culture dish is arranged on the inner side of the corresponding position of the lower mark 21, the dish cover 1 is covered, the plant tissue culture tissue can be just positioned below the convex lens 13, the inoculation is completed, the culture dish is transferred into the incubator, and when in observation, the whole culture dish is taken out, and is observed by naked eyes through the convex lens 13, and the microscopic plant tissue is amplified through the convex lens 13, so that the observation is clearer, the growth condition of the plant tissue can be mastered better, the experiment is carried out smoothly, and the experiment failure caused by secondary pollution due to the fact that the cover opening observation in the ultra-clean workbench is avoided because the operation is simpler.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides an easily observe plant tissue culture dish, includes dish lid (1) and ware body (2), its characterized in that: the height of the dish cover (1) is 8-15mm, a cylindrical air port (12) is arranged in the center of the top of the dish cover (1), a cotton plug (11) is arranged in the air port (12), 4 identical circular convex lenses (13) which are vertically and equidistantly arranged are arranged on the top of the dish cover (1), an upper mark (14) is arranged on the side wall of the dish cover (1), and the upper mark (14) is positioned on the projection of the connecting line of the center of the dish cover (1) and the center of the convex lenses (13); the side wall of the dish body (2) is provided with a lower mark (21), and the included angle between the lower mark (21) and the central connecting line of the dish body (2) is 90 degrees.

2. The easily observable plant tissue culture dish of claim 1, characterized in that: the dish cover (1) and the dish body (2) are made of glass.

3. The easily observable plant tissue culture dish of claim 1, characterized in that: the diameter of the air port (12) is 0.5 cm to 1cm.

4. The easily observable plant tissue culture dish of claim 1, characterized in that: the cotton plug (11) is made of defatted cotton through extrusion molding.

5. The easily observable plant tissue culture dish of claim 1, characterized in that: the diameter of the dish body (2) is 6-15cm.

6. The easily observable plant tissue culture dish of claim 1, characterized in that: the diameter of the dish cover (1) is 5-10mm larger than that of the dish body (2).

7. The easily observable plant tissue culture dish of claim 1, characterized in that: the upper mark (14) is close to the bottom of the side wall of the dish cover (1), and the lower mark (21) is positioned at a position 1-2mm below the dish cover (1) when the dish cover (1) is covered.