CN202379994U - Callus co-culture bottle - Google Patents
Callus co-culture bottle Download PDFInfo
- Publication number
- CN202379994U CN202379994U CN 201120545861 CN201120545861U CN202379994U CN 202379994 U CN202379994 U CN 202379994U CN 201120545861 CN201120545861 CN 201120545861 CN 201120545861 U CN201120545861 U CN 201120545861U CN 202379994 U CN202379994 U CN 202379994U
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- CN
- China
- Prior art keywords
- bottle
- callus
- culture
- culturing
- transparent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a callus co-culture bottle, which is a transparent cylindrical bottle body, wherein the bottle opening of a culture bottle is provided with a bacterium filtering membrane, the bacterium filtering membrane is fastened at the position of the bottle opening through a bottle opening hoop, a bottle cover is in threaded connection with the bottle opening hoop, the bottle cover is provided with air pores, an air supply pipe is inserted into the culture bottle from the bottle cover, a transparent partition board is vertically arranged at the middle of the culture bottle, the partition board is provided with micro pores, the aperture of each micro pore is 4-5 microns, the bottle cover is transparent, and is also provided with micro pores, and the aperture of each micro pore is 4-5microns. The callus co-culture bottle disclosed by the utility model is easy for sterilization, is not easily polluted, has good ventilation function, can effectively prevent the overlapping of the callus during the co-culture, so that good callus co-culture research effect can be achieved.
Description
Technical field:
The utility model relates to the container of plant callus, co-culture of cells, what be specifically related to is a kind of callus culturing bottle altogether.
Background technology:
Callus refers to the isolated organ that scales off from plant materials or is organized under the suitable culture condition to carry out cell fission, formed tissue.This tissue can sprout and root through inducing differentiation.Callus culture simultaneously also is one of the approach that obtains to have the secondary metabolite of activeconstituents.Two kinds of different callus are placed on and place on kind of the substratum that to cultivate in the culture vessel be exactly cultivation altogether.The interaction of research two kind of plant on tissue level often will be adopted callus cultured method altogether.Two kinds of callus being placed on a different positions in the culturing bottle cultivates; Two kinds of callus growth for some time are easy to mutual overlapping; And cause being difficult to observe growing state, differentiation situation separately, say nothing of and collect different callus, analyze the variation of products of cellular metabolism separately.
Summary of the invention:
The purpose of the utility model provides a kind of callus culturing bottle altogether, and it is used to solve the problem of overlapping each other that is easy to when existing callus culture container is cultivated two kinds of callus.
The utility model solves the technical scheme that its technical problem adopted: this callus culturing bottle altogether is transparent round shape bottle; The bottleneck of culturing bottle is provided with the bacterium filtering membrane; The bacterium filtering membrane is fastened on bottle mouth position through the bottleneck hoop; Bottle cap is threaded with the bottleneck hoop, and bottle cap has pore, and blowdown pipe is inserted in the culturing bottle from bottle cap; Upright in the middle of the culturing bottle have a lamina of septum pellucidum, and dividing plate has micropore, 4 ~ 5 microns of micropore sizes; Bottle cap also is transparent, also has micropore, 4 ~ 5 microns of micropore sizes on the bottle cap.
Such scheme median septum thickness is 1 millimeter, highly be the culturing bottle bottle high about 2/3.
Blowdown pipe is made of plastic in the such scheme.
The utlity model has following beneficial effect:
1, the utility model is easy to sterilization, is difficult for polluting, and ventilation is good, can effectively intercept the overlapping of callus when cultivating altogether, can obtain good callus culture studies effect altogether.
2, the utility model is simple in structure, and cost is low, is suitable for medium and small sized enterprises and laboratory and uses.
Description of drawings
Fig. 1 is the structural representation of the utility model.
1. bottle cap, 2. pore, 3. blowdown pipe, 4. dividing plate.
Embodiment
Below in conjunction with accompanying drawing the utility model is done further explanation:
As shown in Figure 1; This callus culturing bottle altogether is transparent round shape bottle; The bottleneck of culturing bottle is provided with the bacterium filtering membrane, and the bacterium filtering membrane is fastened on bottle mouth position through the bottleneck hoop, and the bottleneck hoop is the little cylinder of both ends open; Screw thread is all arranged on the inwall of bottleneck hoop and the outer wall, and bottle cap 1 is threaded with the bottleneck hoop; Bottle cap 1 has pore 2, and blowdown pipe 3 is inserted in the culturing bottle from bottle cap 1, and blowdown pipe 3 is made of plastic; Upright in the middle of the culturing bottle have a lamina of septum pellucidum 4, and dividing plate 4 thickness are 1 millimeter, highly be bottle high by about 2/3, and dividing plate 4 has micropore, 4 ~ 5 microns of micropore sizes, and the size that the big or small selection of micropore size is based on vegetable cell is generally more than 20 microns; Bottle cap 1 also be transparent and its on micropore is arranged, 4 ~ 5 microns of micropore sizes.
Culturing bottle is transparent; Such culturing bottle is convenient to observe callus Growth, differentiation situation in the bottle, and pore 2 on the bottle cap 1 and bacterium filtering membrane can make things convenient for the turnover of air; The utility model simultaneously can stop the entering of mikrobe again, so need not extraly provide sterile air.In addition; Blowdown pipe 3 on the culturing bottle bottle cap 1 is the inlets that add gases such as oxygen, carbonic acid gas, ethene to culturing bottle; Convenient these gases of research influence different callus growths, differentiation and secondary metabolite synthetic under cultivation situation altogether; When not adding these gases, this blowdown pipe 3 can arrive the effect of gas air exchange equally.Also have, culturing bottle can independently place the high pressure moist heat sterilizer to sterilize, and guarantees sterilising effect easily.
Dividing plate 4 in the utility model is had the micropore material and is formed by transparent, and selecting transparent material is for logical light.The effect of being convenient to different light, light quality when cultivating altogether is unaffected.Can stop plant callus when cultivating altogether, to overlap through dividing plate, can not influence callus again through producing the effect of counteracting each other that allelochemical takes place.
The utility model can be cultivated altogether to callus and bring great convenience.During use substratum is added, sterilizes, insert different callus respectively in central baffle 4 both sides after the condensation, add a cover cultivation and get final product.
Claims (3)
1. a callus is total to culturing bottle; It is characterized in that: this callus culturing bottle altogether is transparent round shape bottle; The bottleneck of culturing bottle is provided with the bacterium filtering membrane, and the bacterium filtering membrane is fastened on bottle mouth position through the bottleneck hoop, and bottle cap (1) is threaded with the bottleneck hoop; Bottle cap (1) has pore (2), and blowdown pipe (3) is inserted in the culturing bottle from bottle cap (1); Upright in the middle of the culturing bottle have a lamina of septum pellucidum (4), and dividing plate (4) has micropore, 4 ~ 5 microns of micropore sizes; Bottle cap (1) also is transparent, and bottle cap (1) is gone up also has micropore, 4 ~ 5 microns of micropore sizes.
2. callus according to claim 1 is culturing bottle altogether, and it is characterized in that: described dividing plate (4) thickness is 1 millimeter, highly be the culturing bottle bottle high about 2/3.
3. callus according to claim 1 is culturing bottle altogether, and it is characterized in that: described blowdown pipe (3) is made of plastic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120545861 CN202379994U (en) | 2011-12-23 | 2011-12-23 | Callus co-culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120545861 CN202379994U (en) | 2011-12-23 | 2011-12-23 | Callus co-culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202379994U true CN202379994U (en) | 2012-08-15 |
Family
ID=46628511
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120545861 Expired - Fee Related CN202379994U (en) | 2011-12-23 | 2011-12-23 | Callus co-culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202379994U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114149919A (en) * | 2021-12-02 | 2022-03-08 | 鲁东大学 | A culture apparatus for cassava genetic information conversion |
| CN115530072A (en) * | 2022-09-02 | 2022-12-30 | 广西壮族自治区林业科学研究院 | Culture bottle for solving browning of African daisy culture material |
-
2011
- 2011-12-23 CN CN 201120545861 patent/CN202379994U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114149919A (en) * | 2021-12-02 | 2022-03-08 | 鲁东大学 | A culture apparatus for cassava genetic information conversion |
| CN114149919B (en) * | 2021-12-02 | 2023-06-23 | 鲁东大学 | Culture device for cassava genetic information transformation |
| CN115530072A (en) * | 2022-09-02 | 2022-12-30 | 广西壮族自治区林业科学研究院 | Culture bottle for solving browning of African daisy culture material |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120815 Termination date: 20121223 |