CN220555691U

CN220555691U - Cell culture isolator

Info

Publication number: CN220555691U
Application number: CN202321928764.8U
Authority: CN
Inventors: 吴凯; 张宏; 殷健峰; 丁鹏; 商佳成
Original assignee: Sairuiyi Jiangsu Biological Equipment Co ltd
Current assignee: Sairuiyi Jiangsu Biological Equipment Co ltd
Priority date: 2023-07-21
Filing date: 2023-07-21
Publication date: 2024-03-05
Anticipated expiration: 2033-07-21

Abstract

The utility model belongs to the technical field of cell culture, in particular to a cell culture isolator, which comprises an isolation box, wherein the upper surface of the isolation box is provided with plug holes, the upper part of the inner wall of the isolation box is fixedly provided with symmetrically distributed ultraviolet sterilization lamps I, one side surface of the inside of the isolation box is fixedly provided with a temperature sensor, the lower surface of the inside of the isolation box is connected with a culture box, the lower part of the surface of one side of the isolation box is provided with a motor groove, the inner wall of the motor groove is provided with a constant temperature mechanism, one side of the inner layer of the isolation box is provided with an air cavity, and the upper surface of the inner wall of the air cavity is provided with vent holes which are distributed equidistantly. The utility model can filter dust in air by utilizing the filtering function of the air filter and can disinfect the filtered air by utilizing the air purifier when in use, thereby preventing the environmental damage in the isolation box.

Description

Cell culture isolator

Technical Field

The utility model relates to the technical field of cell culture, in particular to a cell culture isolator.

Background

Cell culture refers to a method for simulating in vitro in vivo environment (asepsis, proper temperature, pH value, certain nutrition condition, etc.) to survive, grow, reproduce and maintain main structure and function; cell culture is an indispensable process for the whole bioengineering technology or one of the biological cloning technologies, and is a large-scale cloning of cells; in order to keep the cells in a sterile environment during cell culture, it is necessary to use an isolator for storage culture.

The isolator on the current market (for example, patent number 202222206010.3, the isolator is provided with a transmission mechanism, when sampling cells in a culture dish, the transmission mechanism is matched with a telescopic plate and a manipulator, the isolator can keep a sterile environment when taking things, external air can not enter due to taking things, the internal environment of the isolator is polluted, and meanwhile, a pulley is provided, so that the whole isolator can be conveniently moved and positioned, and workers can flexibly operate) has the following problems:

(1) When a cell sample is taken and placed, external air can enter the isolator, so that the environment inside the isolator is polluted;

(2) When the cell isolation culture is carried out, the cell can be rapidly propagated and grown in a proper environment, and the existing isolation box solves the temperature problem, but the environment inside the isolation box is polluted along with the entering of the outside air.

Disclosure of Invention

The utility model aims to solve the defects in the prior art and provides a cell culture isolator.

In order to achieve the above purpose, the present utility model adopts the following technical scheme:

the utility model provides a cell culture isolator, includes the isolation box, the spliced eye has been seted up to the upper surface of isolation box, and the inner wall upper portion of isolation box is fixed with symmetrical distribution's ultraviolet sterilizing lamp one, the inside one side fixed surface of isolation box has the temperature sensor who sets up, and the inside lower surface of isolation box is connected with the cultivation box that sets up, motor groove has been seted up to isolation box one side surface lower part, and the constant temperature mechanism of setting is installed to motor groove inner wall, the wind chamber has been seted up to isolation box inlayer one side, and the exhaust hole that the equidistance distributes has been seted up to wind chamber inner wall upper surface.

As still further scheme of the utility model, the constant temperature mechanism comprises an air filter connected with the inner wall of one side of the motor groove through a fan housing, the air filter is connected with an air compressor through a connecting pipe, the air compressor is connected with a condenser through a connecting pipe, the condenser is connected with an expansion valve through a connecting pipe, and the expansion valve is connected with a heat exchanger through a connecting pipe.

As a still further scheme of the utility model, the heat exchanger is connected with an air purifier through a connecting pipe, and the air purifier is connected with the air cavity through the connecting pipe.

As a still further proposal of the utility model, the inner wall of the plug hole is plugged with a conveying mechanism which is arranged, and the conveying mechanism comprises a plug sleeve.

As a still further scheme of the utility model, the inner wall of the plug bush is fixedly provided with a conveying pipe, the upper part of the surface of the conveying pipe is fixedly provided with a solenoid valve, the upper surface of the conveying pipe is fixedly provided with a fixed seat, the outer wall of the fixed seat is fixedly provided with a box body, and the inner wall of the box body is fixedly provided with ultraviolet sterilizing lamps II which are symmetrically distributed.

As a still further proposal of the utility model, an air inlet is arranged on the inner wall of one side of the motor groove, and the inner wall of the air inlet is fixedly provided with a fan which is connected with the inner wall of the fan housing in a penetrating way.

As a still further scheme of the utility model, the temperature sensor is connected with a controller group through a signal wire, and the controller group is connected with the ultraviolet sterilizing lamp I, the air compressor, the condenser, the expansion valve, the heat exchanger, the air purifier, the electromagnetic valve and the ultraviolet sterilizing lamp II through the signal wire.

As a still further scheme of the utility model, the temperature sensor, the first ultraviolet sterilizing lamp, the air compressor, the condenser, the expansion valve, the heat exchanger, the air purifier, the electromagnetic valve and the second ultraviolet sterilizing lamp are connected with an external switch which is connected with a set external power supply through a wire.

The beneficial effects of the utility model are as follows:

1. according to the cell culture isolator, after the external air enters the isolation box through the ultraviolet sterilizing lamp arranged in the isolation box, the environment inside the isolation box is disinfected by utilizing the sterilizing function of the ultraviolet sterilizing lamp, meanwhile, the cell culture isolator can be used without storing, a culture dish filled with cell sap is connected with a conveying pipe, and an electromagnetic valve is opened for discharging after the interior of the box is disinfected by utilizing the ultraviolet sterilizing lamp II;

2. according to the cell culture isolator, through the air filter and the air purifier, when the cell culture isolator is used, dust in air can be filtered by utilizing the filtering function of the air filter, and meanwhile, the filtered air can be disinfected by utilizing the air purifier, so that the environment inside the isolation box is prevented from being damaged;

3. the cell culture isolator of this design is pegged graft mutually through pegging graft downthehole wall and grafting cover outer wall, can conveniently dismantle conveying mechanism when using to can guarantee conveying mechanism's cleanliness in the use, avoid causing the pollution to the cell.

Drawings

FIG. 1 is a front view showing the overall structure of a cell culture isolator according to the present utility model;

FIG. 2 is a cross-sectional view of a cell culture isolator according to the present utility model;

FIG. 3 is a front view of the structure of a thermostatic mechanism of a cell culture isolator according to the present utility model;

FIG. 4 is a front view of the structure of a conveying mechanism of a cell culture isolator according to the present utility model.

In the figure: 1. an isolation box; 2. a plug hole; 3. an ultraviolet sterilizing lamp I; 4. a temperature sensor; 5. a motor slot; 6. a wind chamber; 7. an exhaust hole; 8. a culture box; 9. an air filter; 10. an air compressor; 11. a condenser; 12. an expansion valve; 13. a heat exchanger; 14. an air purifier; 15. a plug bush; 16. a delivery tube; 17. an electromagnetic valve; 18. a fixing seat; 19. a case; 20. and an ultraviolet sterilizing lamp II.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.

Example 1:

in order to clean impurities, as shown in fig. 1-4, the cell culture isolator comprises an isolation box 1, wherein plug holes 2 are formed in the upper surface of the isolation box 1, ultraviolet sterilization lamps I3 which are symmetrically distributed are fixed on the upper part of the inner wall of the isolation box 1, a temperature sensor 4 is fixedly arranged on one side surface inside the isolation box 1, a culture box 8 is connected to the lower surface inside the isolation box 1, a motor groove 5 is formed in the lower part of one side surface of the isolation box 1, a constant temperature mechanism is arranged on the inner wall of the motor groove 5, a wind cavity 6 is formed in one side of the inner layer of the isolation box 1, and vent holes 7 which are equidistantly distributed are formed in the upper surface of the inner wall of the wind cavity 6;

the incubator 8 is placed inside the isolation box 1, then the switch of the ultraviolet sterilizing lamp I3 is turned on to sterilize the inside of the isolation box 1, and when cell culture is carried out, the constant temperature mechanism can discharge the treated air into the air cavity 6 and into the isolation box 1 by utilizing the opened exhaust hole 7.

The constant temperature mechanism comprises an air filter 9 connected with the inner wall of one side of the motor groove 5 through a fan housing, the air filter 9 is connected with an air compressor 10 arranged through a connecting pipe, the air compressor 10 is connected with a condenser 11 arranged through a connecting pipe, the condenser 11 is connected with an expansion valve 12 arranged through a connecting pipe, the expansion valve 12 is connected with a heat exchanger 13 arranged through a connecting pipe, the heat exchanger 13 is connected with an air purifier 14 arranged through a connecting pipe, the air purifier 14 is connected with the air cavity 6 through a connecting pipe, an air inlet is formed in the inner wall of one side of the motor groove 5, an air inlet inner wall is fixedly provided with a fan, and the inner wall of the air inlet is connected with the inner wall of the fan housing in a penetrating manner;

the outside air enters the air filter 9 through the fan to be filtered, is then conveyed through the air compressor 10, and finally enters the air purifier 14 to be sterilized after passing through the heat exchanger 13, the expansion valve 12 and the condenser 11.

The temperature sensor 4 is connected with a controller group through a signal wire, the controller group is connected with a first ultraviolet sterilizing lamp 3, an air compressor 10, a condenser 11, an expansion valve 12, a heat exchanger 13, an air purifier 14, an electromagnetic valve 17 and a second ultraviolet sterilizing lamp 20 through signal wires, the temperature sensor 4, the first ultraviolet sterilizing lamp 3, the air compressor 10, the condenser 11, the expansion valve 12, the heat exchanger 13, the air purifier 14, the electromagnetic valve 17 and the second ultraviolet sterilizing lamp 20 are connected with an external switch through wires, and the switch is connected with an external power supply through wires;

the temperature inside the isolation box 1 can be detected by the provided temperature sensor 4, and the safety of use can be ensured at the time of use by the provided switch.

When the cell culture device is used, the culture box 8 is placed in the isolation box 1, then the switch of the ultraviolet sterilizing lamp I3 is turned on to sterilize the interior of the isolation box 1, when cell culture is carried out, external air enters the air filter 9 through the fan to be filtered, then is conveyed through the air compressor 10, finally enters the air purifier 14 to be sterilized after being treated through the heat exchanger 13, the expansion valve 12 and the condenser 11, and is discharged into the air cavity 6 and is discharged into the isolation box 1 through the opened exhaust hole 7, and when the cell culture device is used, the temperature inside the isolation box 1 can be detected through the set temperature sensor 4.

Example 2:

as shown in fig. 1 to 4, this example was supplemented with the following on the basis of example 1: the inner wall of the plug hole 2 is plugged with a conveying mechanism, the conveying mechanism comprises a plug sleeve 15, a conveying pipe 16 is fixedly arranged on the inner wall of the plug sleeve 15, an electromagnetic valve 17 is fixedly arranged on the upper part of the surface of the conveying pipe 16, a fixing seat 18 is fixedly arranged on the upper surface of the conveying pipe 16, a box 19 is fixedly arranged on the outer wall of the fixing seat 18, and ultraviolet sterilizing lamps II 20 which are symmetrically distributed are fixedly arranged on the inner wall of the box 19;

when the cell culture dish is used, the culture dish filled with the cell liquid is connected with the conveying pipe 16, the electromagnetic valve 17 is opened after the interior of the box 19 is disinfected by the ultraviolet sterilization lamp II 20 and then is discharged into the culture box 8, after the cell culture dish is used, the cell culture dish is plugged with the outer wall of the plug bush 15 through the inner wall of the plug hole 2, and the conveying mechanism can be conveniently detached when the cell culture dish is used, so that the cleanliness of the conveying mechanism can be ensured when the cell culture dish is used, and pollution to cells is avoided.

The foregoing is only a preferred embodiment of the present utility model, but the scope of the present utility model is not limited thereto, and any person skilled in the art, who is within the scope of the present utility model, should make equivalent substitutions or modifications according to the technical scheme of the present utility model and the inventive concept thereof, and should be covered by the scope of the present utility model.

Claims

1. The utility model provides a cell culture isolator, its characterized in that, including isolation case (1), spliced eye (2) have been seted up to the upper surface of isolation case (1), and the inner wall upper portion of isolation case (1) is fixed with ultraviolet sterilizing lamp one (3) of symmetric distribution, the inside one side fixed surface of isolation case (1) has temperature sensor (4) that set up, and the inside lower surface connection of isolation case (1) has cultivation box (8) that set up, motor groove (5) have been seted up to isolation case (1) one side surface lower part, and the constant temperature mechanism of setting is installed to motor groove (5) inner wall, wind chamber (6) have been seted up to isolation case (1) inlayer one side, and wind chamber (6) inner wall upper surface has seted up exhaust hole (7) of equidistance distribution.

2. A cell culture isolator according to claim 1, characterized in that the thermostatic mechanism comprises an air filter (9) connected to one side inner wall of the motor tank (5) through a fan housing, the air filter (9) is connected with a set air compressor (10) through a connecting pipe, and the air compressor (10) is connected with a set condenser (11) through a connecting pipe, the condenser (11) is connected with a set expansion valve (12) through a connecting pipe, and the expansion valve (12) is connected with a set heat exchanger (13) through a connecting pipe.

3. A cell culture isolator according to claim 2, characterized in that the heat exchanger (13) is connected with an air cleaner (14) arranged by a connecting pipe, and the air cleaner (14) is connected with the wind chamber (6) by a connecting pipe.

4. A cell culture isolator according to claim 1, characterized in that the inner wall of the insertion hole (2) is inserted with a conveying mechanism, and the conveying mechanism comprises an insertion sleeve (15).

5. The cell culture isolator according to claim 4, wherein a conveying pipe (16) is fixed on the inner wall of the plug bush (15), an electromagnetic valve (17) is fixed on the upper surface of the conveying pipe (16), a fixing seat (18) is fixed on the upper surface of the conveying pipe (16), a box body (19) is fixed on the outer wall of the fixing seat (18), and ultraviolet sterilizing lamps II (20) distributed symmetrically are fixed on the inner wall of the box body (19).

6. The cell culture isolator according to claim 1, wherein an air inlet is formed in an inner wall of one side of the motor groove (5), and a fan is fixedly arranged on the inner wall of the air inlet, and the inner wall of the air inlet is connected with the inner wall of the fan housing in a penetrating manner.

7. The cell culture isolator according to claim 1, wherein the temperature sensor (4) is connected with a controller group through a signal line, and the controller group is connected with a first ultraviolet sterilizing lamp (3), an air compressor (10), a condenser (11), an expansion valve (12), a heat exchanger (13), an air purifier (14), a solenoid valve (17) and a second ultraviolet sterilizing lamp (20) through signal lines.

8. The cell culture isolator according to claim 1, wherein the temperature sensor (4), the first ultraviolet sterilizing lamp (3), the air compressor (10), the condenser (11), the expansion valve (12), the heat exchanger (13), the air purifier (14), the electromagnetic valve (17) and the second ultraviolet sterilizing lamp (20) are connected with an external switch which is connected with a set through a wire, and the switch is connected with an external power supply which is set through a wire.