CN112516357B - Disinfection cabinet is used in bacterin production - Google Patents

Disinfection cabinet is used in bacterin production Download PDF

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Publication number
CN112516357B
CN112516357B CN202011345298.1A CN202011345298A CN112516357B CN 112516357 B CN112516357 B CN 112516357B CN 202011345298 A CN202011345298 A CN 202011345298A CN 112516357 B CN112516357 B CN 112516357B
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CN
China
Prior art keywords
disinfection cabinet
cabinet body
sterilizer
centrifugal fan
peroxyacetic acid
Prior art date
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CN202011345298.1A
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Chinese (zh)
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CN112516357A (en
Inventor
安有才
张宏栋
李琴
宋庆丽
姜洪艳
刘凯
马亮亮
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AB&B Bio Tech Co Ltd JS
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AB&B Bio Tech Co Ltd JS
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Publication of CN112516357A publication Critical patent/CN112516357A/en
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Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
    • A61L2/00Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/16Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
    • A61L2/22Phase substances, e.g. smokes, aerosols or sprayed or atomised substances
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
    • A61L2/00Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/26Accessories or devices or components used for biocidal treatment
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D46/00Filters or filtering processes specially modified for separating dispersed particles from gases or vapours
    • B01D46/10Particle separators, e.g. dust precipitators, using filter plates, sheets or pads having plane surfaces
    • B01D46/12Particle separators, e.g. dust precipitators, using filter plates, sheets or pads having plane surfaces in multiple arrangements

Abstract

The invention discloses a disinfection cabinet for vaccine production, which comprises a disinfection cabinet body, wherein the front side and the back side of the disinfection cabinet body are both provided with an inlet and an outlet, and are provided with pneumatic sealing doors, the inner bottom wall and the inner top wall of the disinfection cabinet body are both provided with peroxyacetic acid nozzles, the inner side wall of the disinfection cabinet body is provided with a flow distribution plate, the upper surface of the disinfection cabinet body is provided with a centrifugal fan, the air inlet end of the centrifugal fan is provided with a filter box, and the output end of the centrifugal fan is communicated with the flow distribution plate inside the right end of the disinfection cabinet body through a pipeline. This sterilizer is used in bacterin production, through open-ended sterilizer body and pneumatic sealing door around setting up, make egg frame car can carry the inside that the chicken embryo got into the sterilizer body, make the inside peroxyacetic acid of peroxyacetic acid storage bucket pass through measuring pump and compressed air mixture through setting up the peroxyacetic acid nozzle, spray the inside of the sterilizer body, adopt the mode of spraying and air current to disinfect, thereby can be quick, even disinfect on the surface of every chicken embryo, it is swift, high-efficient.

Description

Disinfection cabinet is used in bacterin production
Technical Field
The invention relates to the technical field of vaccine production, in particular to a disinfection cabinet for vaccine production.
Background
The method for culturing influenza virus by using chick embryos is a mature means in the field of influenza virus immunity research, and is widely applied to vaccine research and development and production work. The major influenza vaccine manufacturers worldwide are still based on chicken embryos. Each chick embryo is a closed sterile bioreactor, and the cost can be very low by matching with the highly industrialized breeding industry.
For example, chinese patent application No. CN2008801089689 discloses a method for infecting chicken embryos of immunized hens with influenza virus and obtaining influenza virus suitable for use as an influenza vaccine. For another example, chinese patent application No. CN2004800110059 discloses a method for preparing an influenza virus composition using chicken embryos.
When the chick embryos enter the production area, the surfaces of the chick embryos need to be disinfected by disinfectant to prevent bacteria from remaining, so that the safety and the high efficiency of vaccine production and research and development are ensured. At present, as thousands of chick embryos are used in vaccine production and research and development, the surface disinfection work of the chick embryos is heavier and heavier, and efficient, quick and thorough disinfection equipment is urgently needed.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a disinfection cabinet for vaccine production.
In order to achieve the purpose, the invention adopts the following technical scheme:
a disinfection cabinet for vaccine production comprises a disinfection cabinet body, wherein the front and the back of the disinfection cabinet body are respectively provided with an inlet and an outlet, and are provided with a pneumatic sealing door, peroxyacetic acid nozzles are respectively arranged on the inner bottom wall and the inner top wall of the disinfection cabinet body, a splitter plate is arranged on the inner side wall of the disinfection cabinet body, a centrifugal fan is arranged on the upper surface of the disinfection cabinet body, a filter box is arranged at the air inlet end of the centrifugal fan, the output end of the centrifugal fan is communicated with a splitter plate A inside the right end of the disinfection cabinet body through a pipeline, a residual air exhaust fan is arranged on the upper surface of the disinfection cabinet body, a right-angle pipe is fixedly connected with the input end of the residual air exhaust fan, the front and the back of the right-angle pipe are communicated with a splitter plate B inside the left end of the disinfection cabinet body through a connecting pipeline, the right-angle pipe is connected with the air inlet end of the centrifugal fan, and the connecting part of the right-angle pipe, the residual air exhaust fan and the centrifugal fan is provided with a pneumatic butterfly valve, the peroxyacetic acid nozzle is connected with a metering pump through a liquid inlet pipe, the metering pump is fixedly connected with a peroxyacetic acid storage barrel through a hose, and the liquid inlet pipe is connected with a compressed air input pipe.
Preferably, the inside of the filter box is provided with a G4 grade primary effect filter plate and a F7 grade intermediate effect filter plate, and the F7 grade intermediate effect filter plate is close to the air outlet of the filter box.
Preferably, transparent toughened glass is embedded in the surface of the pneumatic sealing door, and auxiliary frame plates are arranged at the bottom ends of the front surface and the back surface of the disinfection cabinet body.
Preferably, the front of the disinfection cabinet body is embedded with a PLC control screen, the PLC control screen is integrated with a PLC control system, the disinfection cabinet body is internally provided with a peracetic acid concentration sensor, a temperature probe and a temperature controller, and the PLC control system is respectively coupled with the peracetic acid concentration sensor, the temperature probe and the temperature controller.
Preferably, the upper surface of the disinfection cabinet body is provided with a respirator communicated with the interior of the disinfection cabinet body, and the interior of the respirator is provided with a F7-grade medium-effect filter plate.
Preferably, the inner bottom wall of the disinfection cabinet body is connected with a recovery pipe, and the recovery pipe extends to the outside of the disinfection cabinet body and is communicated with the peroxyacetic acid recovery barrel.
The invention has the following beneficial effects: 1. according to the disinfection cabinet for vaccine production, the disinfection cabinet body with the front opening and the rear opening and the pneumatic sealing door are arranged, so that the egg rack vehicle can carry the chicken embryos into the disinfection cabinet body, the peracetic acid in the peracetic acid storage barrel is mixed with the compressed air through the metering pump by the peracetic acid nozzle and sprayed into the disinfection cabinet body, and the disinfection cabinet is disinfected in a spraying and air flow mode, so that the surface of each chicken embryo can be quickly and uniformly sterilized, and the disinfection cabinet is quick and efficient.
2. According to the disinfection cabinet for vaccine production, the F7-grade medium-effect filter plate and the G4-grade primary-effect filter plate are arranged in the filter box, so that air entering the disinfection cabinet body from the outside is fully filtered, dust and other impurities are prevented from entering the disinfection cabinet body, and the air quality is improved.
Drawings
Fig. 1 is a schematic perspective view of a disinfection cabinet for vaccine production according to the present invention.
Fig. 2 is a schematic front view of a disinfection cabinet for vaccine production according to the present invention.
Fig. 3 is a left-side view structural schematic diagram of a disinfection cabinet for vaccine production according to the present invention.
Fig. 4 is a schematic top view of a disinfection cabinet for vaccine production according to the present invention.
FIG. 5 is a schematic diagram of PID of a disinfection cabinet for vaccine production according to the present invention.
Fig. 6 is an explanatory table of reference numerals in fig. 5.
Fig. 7 is an auxiliary explanatory view of the sterilization method.
In the figure: the device comprises a disinfection cabinet body 1, a pneumatic sealing door 2, a centrifugal fan 3, a filter box 4, a residual fan 5, a right-angle pipe 6, a connecting pipeline 7, a pneumatic butterfly valve 8, a compressed air input pipe 9, an auxiliary frame plate 10, a PLC control screen 11 and a respirator 12.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Referring to fig. 1-6, a disinfection cabinet for vaccine production comprises a disinfection cabinet body 1, wherein the front and the back of the disinfection cabinet body 1 are both provided with an inlet and an outlet, and is provided with a pneumatic sealing door 2, peroxyacetic acid nozzles Q1 and Q2 are respectively arranged on the inner bottom wall and the inner top wall of the disinfection cabinet body 1, a splitter plate is arranged on the inner side wall of the disinfection cabinet body 1, a centrifugal fan 3 is arranged on the upper surface of the disinfection cabinet body 1, a filter box 4 is arranged at the air inlet end of the centrifugal fan 3, a G4-grade primary-effect filter plate H1 and a F7-grade intermediate-effect filter plate H2 are arranged in the filter box 4, and F7 level medium effect filter board H2 is close to the air outlet of rose box 4, and the upper surface of sterilizer 1 is provided with respirator 12 rather than inside intercommunication, and the inside of respirator 12 is provided with F7 level medium effect filter board H3, and the surface of pneumatic sealing door 2 is inlayed and is equipped with transparent toughened glass, and the bottom of the front and the back of sterilizer 1 all is provided with supplementary frame plate 10.
This sterilizer is used in bacterin production, through setting up 4 inside F7 level medium efficiency filter H2 of rose box and G4 level primary efficiency filter H1, make the air that the external world got into 1 inside of sterilizer body fully filtered to avoid getting into debris such as dust, improve the air quality.
The front of sterilizer body 1 inlays and is equipped with PLC control screen 11, and PLC control screen 11 integration has PLC control system, and the internally mounted of sterilizer body 1 has peracetic acid concentration sensor X, temperature probe T0 and temperature controller T1, and PLC control system is coupled with peracetic acid concentration sensor X, temperature probe T0, temperature controller T1 respectively.
Centrifugal fan 3's output passes through the inside flow distribution plate A of right-hand member of the pipeline with disinfection cabinet body 1 and communicates, disinfection cabinet body 1's last surface mounting has row incomplete fan 5, arrange incomplete fan 5 input end fixedly connected with right angle pipe 6, the front and the back of right angle pipe 6 communicate through connecting tube 7 and the inside flow distribution plate B of disinfection cabinet body 1 left end, right angle pipe 6 is connected with centrifugal fan 3's inlet end, and right angle pipe 6 and row incomplete fan 5, centrifugal fan 3's junction all is provided with pneumatic butterfly valve 8, peracetic acid nozzle Q1, Q2 is connected with measuring pump B2 through the feed liquor pipe, measuring pump B2 stores bucket Z1 through hose fixedly connected with peracetic acid, the feed liquor union coupling has compressed air input tube 9, disinfection cabinet body 1's inner bottom wall is connected with the recovery tube, the outside and peracetic acid recovery bucket Z2 intercommunication that the recovery tube extends to disinfection cabinet body 1.
This sterilizer is used in bacterin production, through open-ended sterilizer body 1 and pneumatic sealing door 2 around setting up, make egg frame car can carry the inside that the chicken embryo got into sterilizer body 1, through setting up peracetic acid nozzle Q1, Q2 makes the inside peracetic acid of peracetic acid storage bucket Z1 pass through measuring pump B2 and compressed air and mix, spray the inside of sterilizer body 1, the mode that adopts spraying and air current is disinfected, thereby can be quick, the even surface to every chicken embryo disinfects, and is swift, high-efficient.
The disinfection method comprises the following steps: 1. under full load conditions, the disinfection program was run with the following disinfection parameters: the disinfection time is 180-300 s, the residue discharge operation time is 60-360 s, the purging operation time is 120-300 s, and the recovery operation time is 60-300 s;
2. sampling before and after disinfection, namely taking a surface microorganism sample, wherein the sampling area is not less than 25 cm;
3. sampling before disinfection, randomly sampling one chick embryo in each vehicle, taking 4 chick embryos in total, and continuously sampling three batches;
4. sampling after disinfection, wherein sampling points 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 and 12 located in the middle of the upper part and the lower part of each egg rack vehicle (as shown in figure 7 in the specification) are worst points, and continuously sampling three batches according to the sampling positions;
5. the microorganism sampling method comprises the following steps: dividing the culture dish into four pieces, sampling the egg tops of the chicken embryos on four sides, placing the culture dish on the egg top for 10 seconds each time, covering the culture dish with a cover after sampling, taking the culture dish to an incubator for culture, culturing for 72 hours at 20-25 ℃, and then transferring to the incubator for culture for 48 hours at 30-35 ℃.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (6)

1. The utility model provides a sterilizer is used in bacterin production, includes sterilizer body (1), its characterized in that: the front and the back of the disinfection cabinet body (1) are both provided with an inlet and an outlet, and are provided with a pneumatic sealing door (2), peroxyacetic acid nozzles are respectively arranged on the inner bottom wall and the inner top wall of the disinfection cabinet body (1), a splitter plate is arranged on the inner side wall of the disinfection cabinet body (1), a centrifugal fan (3) is arranged on the upper surface of the disinfection cabinet body (1), a filter box (4) is arranged at the air inlet end of the centrifugal fan (3), the output end of the centrifugal fan (3) is communicated with the splitter plate A inside the right end of the disinfection cabinet body (1) through a pipeline, a residual fan (5) is arranged on the upper surface of the disinfection cabinet body (1), a right-angle pipe (6) is fixedly connected with the input end of the residual fan (5), the front and the back of the right-angle pipe (6) are communicated with the splitter plate B inside the left end of the disinfection cabinet body (1) through a connecting pipeline (7), the right angle pipe (6) is connected with the inlet end of centrifugal fan (3), and the junction of right angle pipe (6) and row incomplete fan (5), centrifugal fan (3) all is provided with pneumatic butterfly valve (8), the peroxyacetic acid nozzle has the measuring pump through the feed liquor union coupling, the measuring pump passes through hose fixedly connected with peroxyacetic acid storage bucket, the feed liquor union coupling has compressed air input tube (9).
2. The sterilizer for producing vaccine according to claim 1, wherein: the inside of rose box (4) is provided with G4 level and just imitates the filter in grades with F7, and F7 level is just imitated the filter and is close to the air outlet department of rose box (4) in grades.
3. The sterilizer for producing vaccine according to claim 1, wherein: transparent toughened glass is embedded in the surface of the pneumatic sealing door (2), and auxiliary frame plates (10) are arranged at the front and the bottom of the back of the disinfection cabinet body (1).
4. The sterilizer for producing vaccine according to claim 1, wherein: the front of the disinfection cabinet body (1) is embedded with a PLC control screen (11), the PLC control screen (11) is integrated with a PLC control system, the internal mounting of the disinfection cabinet body (1) is provided with a peracetic acid concentration sensor, a temperature probe and a temperature controller, and the PLC control system is coupled with the peracetic acid concentration sensor, the temperature probe and the temperature controller respectively.
5. The sterilizer for producing vaccine according to claim 1, wherein: the upper surface of the disinfection cabinet body (1) is provided with a respirator (12) communicated with the inside of the disinfection cabinet body, and an F7-grade medium-effect filter plate is arranged inside the respirator (12).
6. The sterilizer for producing vaccine according to claim 1, wherein: the inner bottom wall of the disinfection cabinet body (1) is connected with a recovery pipe, and the recovery pipe extends to the outside of the disinfection cabinet body (1) and is communicated with a peroxyacetic acid recovery barrel.
CN202011345298.1A 2020-11-26 2020-11-26 Disinfection cabinet is used in bacterin production Active CN112516357B (en)

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