CN216891022U - Milk cow liver cell culture and storage device for experiments - Google Patents
Milk cow liver cell culture and storage device for experiments Download PDFInfo
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- CN216891022U CN216891022U CN202220521365.9U CN202220521365U CN216891022U CN 216891022 U CN216891022 U CN 216891022U CN 202220521365 U CN202220521365 U CN 202220521365U CN 216891022 U CN216891022 U CN 216891022U
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- 238000004113 cell culture Methods 0.000 title claims abstract description 10
- 238000002474 experimental method Methods 0.000 title claims abstract description 9
- 210000005229 liver cell Anatomy 0.000 title claims description 12
- 239000008267 milk Substances 0.000 title claims description 6
- 235000013336 milk Nutrition 0.000 title claims description 6
- 210000004080 milk Anatomy 0.000 title claims description 6
- 210000003494 hepatocyte Anatomy 0.000 claims abstract description 21
- 235000013365 dairy product Nutrition 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims description 67
- 230000008878 coupling Effects 0.000 claims description 8
- 238000010168 coupling process Methods 0.000 claims description 8
- 238000005859 coupling reaction Methods 0.000 claims description 8
- 238000004891 communication Methods 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 230000007246 mechanism Effects 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 3
- 238000007599 discharging Methods 0.000 claims 3
- 239000001963 growth medium Substances 0.000 abstract description 5
- 230000004083 survival effect Effects 0.000 abstract description 4
- 230000012010 growth Effects 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract description 2
- 230000008569 process Effects 0.000 abstract description 2
- 230000009471 action Effects 0.000 description 5
- 239000003595 mist Substances 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
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Abstract
The utility model relates to the technical field of cell culture, in particular to a dairy cow hepatocyte culture and storage device for experiments, and provides a scheme for solving the problems that culture solution is inconvenient to replace and culture environment is easy to pollute in the prior art. The culture medium replacing device is reasonable in structure, stable in structure and simple to operate, can effectively prevent hepatocytes from being polluted in the culture process, improves the survival rate of the hepatocytes, facilitates experimenters to observe the growth condition of the hepatocytes, also facilitates culture medium replacement, effectively reduces the labor intensity of the culturers, and is easy to popularize and use.
Description
Technical Field
The utility model relates to the field of cell culture, in particular to a device for culturing and storing hepatic cells of an experimental cow.
Background
The liver is an important organ and plays a very important role in aspects of metabolism, digestion, detoxification, blood coagulation, immunity, regulation and the like, and the liver cells separated from the body are beneficial to in vitro researches such as pharmacological toxicology, immunology, cell biology and the like. The in vitro culture of the hepatocyte has great significance for the research of liver diseases.
Factors such as the humiture of the experiment milk cow liver cell culture to the culture environment among the prior art have great influence to the cell culture, traditional liver cell culture apparatus's air often just enters into the culture environment after simple filtration only, bacterium in the air often can unmanned culture environment, it is lower to cause the liver cell culture survival rate, and when cultivateing the liver cell, need regularly change the culture solution to in a plurality of culture tanks, experimenter's intensity of labour is great, and frequently open culture apparatus and often can introduce the germ in the external air environment, pollute the culture environment, for this reason, this scheme has provided a milk cow liver cell culture and storage device for the experiment.
SUMMERY OF THE UTILITY MODEL
The utility model provides a dairy cow hepatocyte culturing and storing device for experiments, which solves the problems that culture solution is inconvenient to replace and culture environment is easy to be polluted in the prior art.
In order to achieve the purpose, the utility model adopts the following technical scheme:
the utility model provides a milk cow liver cell is cultivateed and storage device for experiments, the power distribution box comprises a box body, the operating door is installed in the front of box, the liquid reserve tank that a plurality of levels set up is installed to the inboard of box, and is a plurality of the through-hole that is linked together with the liquid reserve tank is all seted up at the top of liquid reserve tank, the inboard of box is equipped with a plurality of cultivations jar, and is a plurality of the bottom of cultivations jar runs through a plurality of through-holes respectively and extends to the inboard of liquid reserve tank, the intercommunication mouth has been seted up to the bottom inner wall of cultivations jar, cultivates the inboard bottom of jar and installs the baffle with intercommunication mouth matched with, just the inboard of cultivations jar is equipped with the micropore board, the back rigid coupling of box has the air-collecting cover that the level set up, air supply mechanism is installed to the air-collecting cover.
Preferably, it is a plurality of the both sides outer wall of liquid reserve tank all with the inner wall rigid coupling of box, one side externally mounted of box has the collector tube, install a plurality ofly on the collector tube and correspond the drain pipe that sets up with the liquid reserve tank, it is a plurality of all install the valve on the drain pipe, and the other end of a plurality of drain pipes extends to the inboard bottom of a plurality of liquid reserve tanks respectively, and is a plurality of the feed liquor pipe has all been cup jointed at the one end top of liquid reserve tank, and is a plurality of the feed liquor pipe all extends to the outside of box.
Preferably, the baffle is made for plastic materials, the baffle is located the intercommunication mouth directly over, the bottom rigid coupling of baffle has the connecting rod of vertical setting, and is a plurality of the bottom of connecting rod all runs through the bottom that cultivates the jar and extend to the cultivation jar, and the bottom rigid coupling of a plurality of connecting rods has the floating ring.
Preferably, one end of the culture tank, which is positioned on the inner side of the through hole, is matched with the through hole, and the bottom of the culture tank is fixedly connected with a plurality of support legs.
Preferably, air supply mechanism including install at the inboard sterilamp of wind-collecting cover, cup joint at a plurality of air-supply lines at the wind-collecting cover top, install at the inside heater strip of air-supply line, cup joint the play tuber pipe in wind-collecting cover bottom, install at the inboard fan of a tuber pipe, install the humidifier in the wind-collecting cover below and install the temperature and humidity sensor at the inboard top of box, the other end that goes out the tuber pipe extends to the inboard of box, the shower nozzle of being connected with the humidifier output is installed to the inboard that goes out the tuber pipe, and is a plurality of the equal rigid coupling in inboard top of air-supply line has the filter screen.
Preferably, the box is kept away from one side inner wall rigid coupling of operating door and is had well hollow plate, a plurality of air outlets have been seted up to the outside of well hollow plate, it extends to the inboard of well hollow plate to go out the inboard one end that the tuber pipe is located the box.
The utility model has the beneficial effects that:
1. mutually support through liquid reserve tank, culture tank, micropore board, kickboard, connecting rod, fluid-discharge tube, feed liquor pipe etc. and conveniently change the culture solution of storing in the liquid reserve tank, avoid changing in proper order and supply the culture solution to a plurality of culture tanks, effectually alleviateed cultivateing personnel's intensity of labour, conveniently change the culture solution.
2. Mutually support through liquid reserve tank, culture tank, baffle, kickboard, connecting rod, intercommunication mouth etc. conveniently will cultivate the jar and follow the liver cell of taking out in the liquid reserve tank and cultivating in the jar and observe to when the liquid reserve tank outside is taken out to the culture tank, the intercommunication mouth is closed, prevents that the culture solution in the culture tank from running off, and the convenient growth condition to the liver cell in the culture tank is observed.
3. Through mutually supporting of air-collecting cover, sterilamp, air-supply line, heater strip, play tuber pipe, humidifier, temperature and humidity sensor etc, the convenience is controlled the inboard temperature of box and humidity, provides good growing environment for cell growth, and the air that enters into the box inboard passes through sterilamp sterilization, and then effectively kills the germ that enters into the inboard air of box, avoids the cell of cultivateing to be polluted, improves the survival rate that the hepatocyte cultivateed.
The utility model has reasonable structure, stable structure and simple operation, can effectively avoid the hepatic cells from being polluted in the cultivation process, improves the survival rate of the hepatic cells, is convenient for experimenters to observe the growth condition of the hepatic cells, is also convenient to replace culture solution, effectively lightens the labor intensity of the culturers, and is easy to popularize and use.
Drawings
Fig. 1 is a front sectional structural view of the present invention.
Fig. 2 is a left side sectional structural schematic view of the present invention.
Fig. 3 is an enlarged schematic view of the structure at a in fig. 1 according to the present invention.
Reference numbers in the figures: 1. a box body; 2. an operating door; 3. a liquid storage tank; 4. a liquid outlet pipe; 5. a liquid collecting pipe; 6. a liquid inlet pipe; 7. a through hole; 8. a culture tank; 9. a microporous plate; 10. a communication port; 11. a baffle plate; 12. a connecting rod; 13. a floating ring; 14. a support leg; 15. a hollow plate; 16. an air outlet; 17. a wind collecting cover; 18. an air outlet pipe; 19. a fan; 20. a sterilizing lamp; 21. an air inlet pipe; 22. heating wires; 23. a filter screen; 24. a humidifier; 25. a spray head; 26. temperature and humidity sensor.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1-3, a dairy cow hepatocyte culturing and storing device for experiment comprises a box body 1, an operation door 2 is arranged on the front surface of the box body 1, a plurality of liquid storage tanks 3 horizontally arranged are arranged on the inner side of the box body 1, the outer walls of the two sides of the plurality of liquid storage tanks 3 are fixedly connected with the inner wall of the box body 1, a liquid collecting pipe 5 is arranged on the outer part of one side of the box body 1, a plurality of liquid outlet pipes 4 correspondingly arranged with the liquid storage tanks 3 are arranged on the liquid collecting pipe 5, valves are arranged on the plurality of liquid outlet pipes 4, and the other ends of the liquid outlet pipes 4 respectively extend to the bottoms of the inner sides of the liquid storage tanks 3, the valves on the liquid outlet pipes 4 are opened, the culture solution stored in the corresponding liquid storage tank 3 is discharged to the liquid collecting pipe 5 and is discharged through the liquid collecting pipe 5, feed liquor pipe 6 has all been cup jointed at the one end top of a plurality of liquid reserve tanks 3, and a plurality of feed liquor pipes 6 all extend to the outside of box 1, conveniently supply the culture solution to liquid reserve tank 3 through feed liquor pipe 6.
A plurality of through holes 7 communicated with the liquid storage tanks 3 are arranged at the tops of the liquid storage tanks 3, a plurality of culture tanks 8 are arranged on the inner side of the box body 1, the culture tanks 8 are made of transparent materials, the bottoms of the culture tanks 8 respectively penetrate through the through holes 7 and extend to the inner side of the liquid storage tanks 3, one ends of the culture tanks 8, which are positioned on the inner sides of the through holes 7, are matched with the through holes 7, and a plurality of supporting legs 14 are fixedly connected with the bottom of the culture tank 8, a communicating opening 10 is arranged on the inner wall of the bottom of the culture tank 8, after the culture tank 8 enters the liquid storage tank 3, the culture solution enters the culture tank 8 through the communication port 10, and after the nutrient components in the culture solution are consumed, under the action of osmotic pressure, the nutrient components of the culture solution in the liquid storage tank 3 enter the culture tank 8, be equipped with micropore board 9 in the inboard of culture tank 8, micropore board 9 can bear the weight of hepatocyte, prevents to fall into inside the liquid reserve tank 3 when making it soak the culture solution.
A baffle 11 matched with the communicating opening 10 is arranged at the bottom of the inner side of the culture tank 8, the baffle 11 is made of plastic materials, the baffle 11 is positioned right above the communicating opening 10, the bottom of the baffle 11 is fixedly connected with a connecting rod 12 which is vertically arranged, the bottoms of a plurality of connecting rods 12 extend to the bottom of the culture tank 8 through the culture tank 8, and the bottom of the plurality of connecting rods 12 is fixedly connected with a floating ring 13, when the culture tank 8 is put into the liquid storage tank 3, the floating ring 13 is immersed into the culture solution, under the action of buoyancy, the floating ring 13 floats upwards, so that the bottom of the baffle plate 11 is separated from the inner wall of the bottom of the culture tank 8, the communication port 10 is opened, when the culture tank 8 is taken out of the liquid storage tank 3, under the action of gravity, the floating ring 13 and the baffle plate 11 move downward, so that the bottom of the baffle plate 11 is abutted against the inner wall of the bottom of the culture tank 8, the communication port 10 is closed, and the culture solution in the culture tank 8 is prevented from leaking.
The back of the box body 1 is fixedly connected with a horizontally arranged air collecting hood 17, an air supply mechanism is arranged on the air collecting hood 17 and comprises a sterilizing lamp 20 arranged on the inner side of the air collecting hood 17, a plurality of air inlet pipes 21 sleeved on the top of the air collecting hood 17, a heating wire 22 arranged inside the air inlet pipes 21, an air outlet pipe 18 sleeved on the bottom of the air collecting hood 17, a fan 19 arranged on the inner side of the air outlet pipe 18, a humidifier 24 arranged below the air collecting hood 17 and a temperature and humidity sensor 26 arranged on the inner side top of the box body 1, the other end of the air outlet pipe 18 extends to the inner side of the box body 1, a nozzle 25 connected with the output end of the humidifier 24 is arranged on the inner side of the air outlet pipe 18, a filter screen 23 is fixedly connected on the inner side top of each air inlet pipe 21, a hollow plate 15 is fixedly connected on the inner wall of one side of the box body 1 far away from the operation door 2, a plurality of air outlets 16 are arranged on the outer side of the hollow plate 15, one end of the air outlet pipe 18 positioned on the inner side of the box body 1 extends to the inner side of the hollow plate 15, the fan 19 rotates, after the air is filtered by the filter screen 23, the air is sucked into the inner side of the air collecting hood 17 through the air inlet pipes 21, after microorganisms in the air are killed and disinfected by the sterilizing lamp 20, the air is input into the inner side of the hollow plate 15 through the air outlet pipe 18 and is discharged into the inner side of the box body 1 from the air outlet holes 16, the temperature and humidity sensor 26 arranged on the inner side of the box body 1 is matched with the heating wire 22 and the humidifier 24, when the temperature of the inner side of the box body 1 is lower than a set value, the heating wires 22 are electrified to heat the air entering the inner side of the air collecting hood 17, when the humidity of the inner side of the box body 1 is lower, the humidifier 24 works to generate water mist, the water mist is sprayed into the air outlet pipe 18 from the spray nozzle 25, and the air entering the box body 1 is humidified.
The working principle is as follows: when the culture tank is used, the operation door 2 is opened, the culture tank 8 is taken out, hepatocytes to be cultured are added into the culture tank 8, then the culture tank 8 is placed into the liquid storage tank 3, then the operation door 2 is closed, a culture solution is added into the liquid storage tank 3 through the liquid inlet pipe 6, under the action of buoyancy, the baffle 11 is separated from the bottom of the culture tank 8, the communication port 10 is opened, the culture solution enters the culture tank 8 through the communication port 10, after nutrients in the culture solution are consumed, under the action of osmotic pressure, the nutrients in the culture solution in the liquid storage tank 3 enter the culture tank 8, the microporous plate 9 is arranged on the inner side of the culture tank 8, the microporous plate 9 can bear the hepatocytes, the hepatocytes are immersed into the culture solution and are prevented from falling into the liquid storage tank 3, the power supply is connected, the fan 19 rotates, and after air is filtered through the filter screen 23, the culture medium is sucked into the inner side of an air collecting hood 17 through a plurality of air inlet pipes 21, microorganisms in the air are sterilized through a sterilizing lamp 20, then the microorganisms are input into the inner side of a hollow plate 15 through an air outlet pipe 18 and are discharged into the inner side of a box body 1 through a plurality of air outlets 16, a temperature and humidity sensor 26 detects the temperature and humidity of the air in the inner side of the box body 1, when the temperature in the inner side of the box body 1 is lower than a set value, a plurality of heating wires 22 are electrified to heat the air entering the inner side of the air collecting hood 17, when the humidity in the inner side of the box body 1 is lower, a humidifier 24 works to generate water mist, the water mist is sprayed into the air outlet pipe 18 from a spray nozzle 25 to humidify the air entering the box body 1, when the culture medium in a liquid storage tank 3 needs to be replaced, a valve on a liquid outlet pipe 4 is opened, the culture medium stored in the corresponding liquid storage tank 3 is discharged into a liquid collection pipe 5 and is discharged through the liquid collection pipe 5, after the culture solution flows out, the valve is closed, and the prepared culture solution is added into the liquid storage tank 3 through the liquid inlet pipe 6.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the equipment or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and equivalent alternatives or modifications according to the technical solution of the present invention and the inventive concept thereof should be covered by the scope of the present invention.
Claims (6)
1. The utility model provides a milk cow liver cell culture and storage device for experiments, includes box (1), its characterized in that, operating door (2) are installed in the front of box (1), the liquid reserve tank (3) that a plurality of levels set up are installed to the inboard of box (1), and a plurality of through-hole (7) that are linked together with liquid reserve tank (3) are all seted up at the top of liquid reserve tank (3), the inboard of box (1) is equipped with a plurality of culture tank (8), and a plurality of the bottom of culture tank (8) runs through a plurality of through-hole (7) respectively, and extends to the inboard of liquid reserve tank (3), the bottom inner wall of culture tank (8) has seted up intercommunication mouth (10), and baffle (11) with intercommunication mouth (10) matched with is installed to the inboard bottom of culture tank (8), and the inboard of culture tank (8) is equipped with micropore board (9), the back rigid coupling of box (1) has air-collecting cover (17) that the level set up, and an air supply mechanism is arranged on the air collecting cover (17).
2. The experimental dairy cow hepatocyte culture and storage device according to claim 1, wherein the outer walls of two sides of the plurality of liquid storage tanks (3) are fixedly connected with the inner wall of the box body (1), a liquid collecting pipe (5) is installed outside one side of the box body (1), a plurality of liquid discharging pipes (4) corresponding to the liquid storage tanks (3) are installed on the liquid collecting pipe (5), valves are installed on the plurality of liquid discharging pipes (4), the other ends of the plurality of liquid discharging pipes (4) respectively extend to the bottoms of the inner sides of the plurality of liquid storage tanks (3), a plurality of liquid feeding pipes (6) are sleeved at the tops of one ends of the liquid storage tanks (3), and a plurality of liquid feeding pipes (6) extend to the outside of the box body (1).
3. The experimental dairy cow hepatocyte culture and storage device according to claim 1, wherein the baffle (11) is made of plastic material, the baffle (11) is located right above the communication port (10), the bottom of the baffle (11) is fixedly connected with a vertically arranged connecting rod (12), the bottom of the connecting rods (12) extends to the bottom of the culture tank (8) through the culture tank (8), and the bottom of the connecting rods (12) is fixedly connected with a floating ring (13).
4. The experimental dairy cow hepatocyte culture and storage device according to claim 1, wherein one end of the culture tank (8) located inside the through hole (7) is engaged with the through hole (7), and a plurality of legs (14) are fixed to the bottom of the culture tank (8).
5. The experimental dairy cow hepatocyte culture and storage device according to claim 1, the air supply mechanism comprises a sterilizing lamp (20) arranged on the inner side of the air collecting cover (17), a plurality of air inlet pipes (21) sleeved at the top of the air collecting cover (17), heating wires (22) arranged in the air inlet pipes (21), an air outlet pipe (18) sleeved at the bottom of the air collecting cover (17), a fan (19) arranged on the inner side of the air outlet pipe (18), a humidifier (24) arranged below the air collecting cover (17) and a temperature and humidity sensor (26) arranged at the inner top of the box body (1), the other end of air-out pipe (18) extends to the inboard of box (1), shower nozzle (25) of being connected with humidifier (24) output are installed to the inboard of air-out pipe (18), and are a plurality of the equal rigid coupling in inboard top of air-supply line (21) has filter screen (23).
6. The experimental dairy cow hepatocyte culture and storage device according to claim 5, wherein a hollow plate (15) is fixed to an inner wall of the box (1) far away from the operation door (2), a plurality of air outlets (16) are formed outside the hollow plate (15), and one end of the air outlet pipe (18) located inside the box (1) extends to the inside of the hollow plate (15).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220521365.9U CN216891022U (en) | 2022-03-10 | 2022-03-10 | Milk cow liver cell culture and storage device for experiments |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220521365.9U CN216891022U (en) | 2022-03-10 | 2022-03-10 | Milk cow liver cell culture and storage device for experiments |
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| Publication Number | Publication Date |
|---|---|
| CN216891022U true CN216891022U (en) | 2022-07-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220521365.9U Expired - Fee Related CN216891022U (en) | 2022-03-10 | 2022-03-10 | Milk cow liver cell culture and storage device for experiments |
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| Country | Link |
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| CN (1) | CN216891022U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118064251A (en) * | 2024-03-31 | 2024-05-24 | 江苏岳艾生物科技有限公司 | Biological culture dish |
-
2022
- 2022-03-10 CN CN202220521365.9U patent/CN216891022U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118064251A (en) * | 2024-03-31 | 2024-05-24 | 江苏岳艾生物科技有限公司 | Biological culture dish |
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Granted publication date: 20220705 |