CN211241206U - An integrated plant tissue culture device - Google Patents

Abstract

The utility model relates to the technical field of incubators, in particular to an integrated plant tissue culture device, comprising an incubator body, a box cover and a base; an aeration tube is detachably connected to the lower part of the incubator body, and one end of the aeration tube is detachably connected. It runs through the side wall of the incubator body and extends outward, and the end is connected with an exhaust pump, a number of exhaust holes are opened on the aeration pipe, a liquid discharge port is arranged under the aeration pipe, and the incubator body is located at the end of the aeration pipe. A first perforated plate is also arranged above, a water quality detector is also arranged above the first perforated plate in the incubator body, and a detection probe is arranged at the bottom of the water quality detector; an acousto-optic alarm is arranged on the outer wall of the incubator body. The side wall of the incubator body is also provided with a gas discharge port and a culture liquid addition port. The utility model not only realizes the integrated cultivation of plant cells, but also realizes the replacement of the culture medium without artificially transferring the plants, thereby avoiding the pollution of the plants caused by the artificial replacement of the culture medium.

Description

An integrated plant tissue culture device

technical field

The utility model relates to the technical field of plant tissue culture devices, in particular to an integrated plant tissue culture device.

Background technique

Plant tissue culture, also known as in vitro culture, refers to the separation of desired tissues, organs or cells or protoplasts from the plant body, and cultured under artificially controlled conditions through aseptic operations to obtain regenerated intact plants or to produce economically viable plants. The technology of other products of value.

Different nutrient supplies are required at different stages of plant growth, so different liquid media need to be provided for cultivating plants at different developmental stages. The culturing devices in the prior art often fail to replace the nutrient solution after inoculation. When replacing the nutrient solution, the plants need to be transplanted to different mediums. At this time, the medium needs to be manually replaced and transferred, which not only causes manual consumption, but also easily pollutes the cultivated plants.

Utility model content

In view of the above-mentioned technical deficiencies, the purpose of this utility model is to provide an integrated plant tissue culture device, which not only realizes the integrated cultivation of plant cells, but also realizes the replacement of the culture solution without artificially transferring plants, The pollution of plants caused by manual replacement of the medium is avoided, and the problem of bacterial contamination in the tissue culture device is effectively solved.

In order to solve the above-mentioned technical problems, the utility model adopts the following technical solutions:

An integrated plant tissue culture device comprises an incubator body, a box cover and a base, the top of the incubator body is detachably connected with the box cover, and the bottom of the incubator body is provided with the base;

An aeration tube is detachably connected to the lower part of the incubator body, one end of the aeration tube penetrates the side wall of the incubator body and extends outward, and the end is connected with an exhaust pump, which is located in the incubator body The inside of the aeration pipe is provided with a number of exhaust holes, and the side wall of the incubator body is also provided with a liquid discharge port, and the liquid discharge port is located under the aeration pipe, inside the incubator body. A first perforated plate is also arranged above the aeration pipe, and a water quality detector is also arranged above the first perforated plate in the incubator body, and a vertical direction is arranged at the bottom of the water quality detector. detection probe;

The outer wall of the incubator body is provided with an acousto-optic alarm, and the side wall of the incubator body is also provided with a gas discharge port and a culture solution adding port;

Valves are provided on the liquid discharge port and the culture medium addition port, and the gas discharge port, the culture liquid addition port and the aeration pipe located outside the incubator are all provided with microporous membrane filtration. device.

Preferably, the incubator body is further provided with a horizontal plate below the liquid discharge port, a plurality of electric heating tubes are arranged side by side at the bottom of the horizontal plate, and a photosynthesis lamp is arranged on the inner top of the box cover, The inner side wall of the incubator body is also provided with a cooling chip and a temperature sensor, and the outer side wall of the incubator body is also provided with an operation panel and a controller, the controller is electrically connected with an external power supply, and the controllers are respectively It is electrically connected with the operation panel, the water quality detector, the exhaust pump, the sound and light alarm, the temperature sensor, the electric heating tube and the refrigeration sheet.

Preferably, the outer top of the incubator body is further provided with a cleaning box, one end of a circulation pipe is connected to the cleaning box, and the other end of the circulation pipe penetrates through the top of the incubator body and is connected to the The sprinkler head on the top of the incubator body passes through, the circulation pipe is also provided with a valve, and the cleaning box is provided with a disinfectant inlet.

Preferably, a plurality of hanging rings are arranged side by side on two opposite inner side walls of the incubator body, the aeration pipes are arranged in an arcuate shape and are detachably connected to the plurality of hanging rings, and the aeration pipes extend into the culture The end in the box body is fixedly arranged on the inner side wall of the incubator body.

Preferably, the top of the first multi-well plate is divided into several culture chambers by a plurality of second multi-well plates crossed horizontally and vertically.

Preferably, the pore size of the sieve holes on the first perforated plate is larger than 625 meshes, and the sieve hole diameter of the second perforated plate is the same as that of the first perforated plate.

Preferably, a side wall of the incubator body is further provided with an observation port, and a transparent glass is embedded in the observation port.

Preferably, the bottom of the base is provided with a universal wheel.

Compared with the prior art, the beneficial effects that the utility model has are:

1. The utility model is provided with an aeration pipe, a microporous membrane filter and an exhaust pump. The microporous membrane filter filters the bacteria in the air, and after the filtered clean air enters the incubator body, the culture liquid is blown to flow, At this time, the flowing culture medium is more evenly mixed, so as to fully realize the cultivation of plant tissues and meet the nutritional needs of different plant cells for growth, and the air contains carbon dioxide. At this time, the carbon dioxide carried in the filtered clean air facilitates the respiration of plant cells. , thereby eliminating the need for devices that provide carbon dioxide.

2. The utility model is provided with an electric heating tube, a temperature sensor, a controller and a refrigerating sheet, and the temperature in the incubator body is sensed by the temperature sensor, and the controller controls the temperature in the incubator body within a certain range, thereby realizing the incubator. The constant temperature is convenient for the cultivation of plant tissues.

3. The utility model is provided with a water quality monitor, an acousto-optic alarm device and a detection probe. The water quality monitor can detect whether the water quality in the culture solution has deteriorated through the contact of the detection probe with the culture solution. The alarm will send out an alarm as a prompt, which is convenient to replace the culture medium in time; at the same time, the setting of the drain port also facilitates the replacement of the culture medium for plants in different developmental stages.

4. The utility model is provided with a cleaning box and a spray head. Before inoculation, a disinfectant can be placed in the cleaning box for sterilization and disinfection, and the incubator body can be sterilized and disinfected in an all-round way through the spray head. After the end, sterile water is put into the cleaning box to clean the inside of the incubator body again to ensure that the incubator body is in a sterile state, and then inoculation is performed.

Description of drawings

1 is a schematic structural diagram of Embodiment 1 of an integrated plant tissue culture device of the present invention;

2 is a schematic structural diagram of Embodiment 2 of an integrated plant tissue culture device of the present invention;

3 is a schematic diagram of the external structure of Embodiment 2 of an integrated plant tissue culture device of the present invention;

4 is a top view of the aeration tube in the incubator body in Embodiment 1 of an integrated plant tissue culture device of the present invention;

5 is a top view of the first porous plate in Embodiment 1 of an integrated plant tissue culture device of the present invention;

FIG. 6 is an electrical connection diagram of an integrated plant tissue culture device of the present invention.

Description of reference numbers:

1. Incubator body; 2. Base; 3. Aeration pipe; 4. Exhaust pump; 5. First porous plate; 6. Water quality detector; 7. Detection probe; 8. Sound and light alarm; 9. Exhaust Liquid port; 10. Gas discharge port; 11. Culture medium addition port; 12. Microporous membrane filter; 13. Horizontal plate; 14. Electric heating tube; 15. Refrigeration sheet; 16. Temperature sensor; ; 18, hanging ring; 19, operation panel; 20, controller; 21, second perforated plate; 22, observation port; 23, universal wheel; 24, box cover; 25, cleaning box; 26, flow pipe; 27. Sprinkler head; 28. Disinfectant adding port.

Detailed ways

The following is a detailed description of the preferred embodiment and accompanying drawings 1-2 in conjunction with the accompanying drawings in the embodiments of the present utility model.

Example 1

As shown in Figures 1, 5 and 6, an integrated plant tissue culture device includes an incubator body 1, a box cover 24 and a base 2, and the top of the incubator body 1 is detachably connected with the box cover 24. The bottom of the incubator body 1 is provided with the base 2, one end of the incubator body 1 is rotatably connected with the box cover 24, and the incubator body 1 and the box cover 24 are respectively provided with The two locks are locked, the base 2 is used to support the incubator body 1, and the incubator body 1 can cultivate plant organs, cells or protoplasts; Air pipe 3, one end of the aeration pipe 3 penetrates the side wall of the incubator body 1 and extends outward, and the end is connected with an exhaust pump 4, and the outside air can enter the incubator body 1 through the exhaust pump 4 A microporous membrane filter 12 is arranged in the aeration pipe 3 outside the incubator body 1. The microporous membrane filter 12 can prevent bacteria from the outside air from entering the incubator body 1, and is located in the incubator body 1. The aeration pipe 3 in the incubator body 1 is provided with a number of exhaust holes, and when the air is discharged from the exhaust holes on the aeration pipe 3, the gas blows the culture solution, so that the culture solution flows and mixes, Not only the problem of insufficient or uneven supply of culture medium is avoided, but also the oxygen in the air facilitates the respiration of plant tissues or cells, and several hanging rings 18 are arranged side by side on the two opposite inner walls of the incubator body 1, so The aeration pipes 3 are arranged in an arcuate shape and are detachably connected with a plurality of the hanging rings 18. The aeration pipes 3 arranged in the arcuate shape make the exhaust holes evenly distributed and increase the contact area with the culture solution. The end extending into the incubator body 1 is fixedly arranged on the inner side wall of the incubator body 1 , so that the other end of the aeration pipe 3 is fixed.

The side wall of the incubator body 1 is provided with a liquid discharge port 9 below the aeration pipe 3, and the liquid discharge port 9 is used to discharge the degenerate culture liquid. The top of the trachea 3 is also provided with a first perforated plate 5, and the top of the first perforated plate 5 is divided into a number of culture chambers by a number of second perforated plates 21 that intersect horizontally and vertically. The structure is the same as that of the first multi-hole plate 5, which can not only make the culture liquid flow, but also realize that different cells or tissues are cultivated in different cultivation chambers, and different plants can be cultivated in the cultivation chamber, realizing a variety of and multiple plants. Simultaneous cultivation, and the first multi-well plate 5 can make the culture fluid fluctuate slowly, which not only realizes the uniform mixing of the culture fluid but also avoids damage to plant cells or tissues due to the violent fluctuation of the nutrient solution. The pore size of the upper sieve hole is greater than 625 meshes, so as to prevent the plant cells from falling to the bottom of the first porous plate 5 from the holes provided on the first porous plate 5 during cultivation, and the incubator body 1 is located in the first porous plate 5. A water quality detector 6 is also arranged above the perforated plate 5, an acousto-optic alarm 8 is arranged on the outer wall of the incubator body 1, and a vertical detection probe 7 is arranged at the bottom of the water quality detector 6. 6 is electrically connected to the detection probe 7, the model of the water quality detection device 6 is YL2, the model of the measurement probe 7 is TDS, and the detection end of the detection probe 7 can extend into the culture solution, the water quality detection device 6 and the detection probe 7 Commonly used to monitor whether the culture medium has deteriorated. If the culture medium has deteriorated, the sound and light alarm 8 has played a prompting role. The model of the sound and light alarm 8 is: TGSG-07, water quality detector 6 and detection probe 7 After being electrically connected, it is electrically connected to an external power supply, and the sound and light alarm 8 is electrically connected to an external power supply.

The outer top of the incubator body 1 is also provided with a cleaning box 25 for storing disinfectant. The cleaning box 25 is connected with one end of a circulation pipe 26 , and the other end of the circulation pipe 26 penetrates the incubator body 1 . The top of the incubator body 1 communicates with the sprinkler head 27 connected to the top of the incubator body 1, the circulation pipe 26 is also provided with a valve, and the cleaning box 25 is provided with a disinfectant adding port 28. 28 is tubular and is provided with a valve. Before inoculation, disinfectant can be placed in the cleaning box 25 for sterilization and disinfection, and the incubator body can be sterilized and disinfected in all directions through the spray head 27. After the disinfection is completed , the cleaning box 25 is built with sterile water to clean the inside of the incubator body again to ensure that the incubator body is in a sterile state, and then inoculation is carried out. into the incubator body 1.

The side wall of the incubator body 1 is also provided with a gas discharge port 10 and a culture solution adding port 11. The gas discharge port 10 and the aeration pipe 3 make the air in the incubator body 1 flow, ensuring the incubator body 1. The air in 1 is fresh, and the liquid discharge port 9 and the culture liquid addition port 11 are all provided with valves. When the liquid discharge port 9 discharges the metamorphic culture liquid or the culture liquid addition port 11 adds fresh culture liquid, the Just open the corresponding valve, the gas discharge port 10 and the culture solution adding port 11 are also provided with a microporous membrane filter 12, and the microporous membrane filter 12 can prevent bacteria in the outside air from entering the incubator body 1 , to prevent the bacteria in the outside air from entering the incubator body 1 through the gas discharge port 10 or the culture solution adding port 11 .

Example 2

As shown in FIG. 2-FIG. 4, the difference from the above-mentioned embodiment is that the incubator body 1 is also sealed with a transverse plate 13 below the liquid discharge port 9, and the bottom of the transverse plate 13 is sealed. A number of electric heating tubes 14 are arranged side by side, and the horizontal plate 13 avoids the problems that the electric heating tubes 14 are in contact with the culture solution and cause the culture solution to deteriorate and the electric heating tubes 14 to be corroded. The plate 15 and the temperature sensor 16, and the liquid level of the culture solution is higher than the temperature sensor 16 and the refrigeration plate 15. The temperature sensor 16 is used to sense the temperature of the culture solution, and the refrigeration plate 15 and the electric heating tube 14 are used together to adjust the temperature of the culture solution. temperature, the inner top of the box cover 24 is provided with a photosynthesis lamp 17 to facilitate the photosynthesis of plant tissues or cells.

The side wall of the incubator body 1 is also provided with an observation port 22. The observation port 22 is embedded with a transparent glass, which is convenient for observing the growth of plants in the incubator body 1. The bottom of the base 2 is provided with a universal The wheel 23 is provided, and the exhaust pump 4 is located on the top of the base 2 to facilitate the movement of the incubator body 1 and the base 2 .

The incubator body 1 is also provided with a transverse plate 13 located below the liquid discharge port 9 , and a plurality of electric heating pipes 14 are arranged side by side at the bottom of the transverse plate 13 , and the inner side wall of the incubator body 1 is also provided with There are refrigeration sheets 15 and a temperature sensor 16, and an operation panel 19 and a controller 20 are also provided on the outer wall of the incubator body 1. The model of the operation panel 19 is: LL86-4J-LN, and the model of the controller 20 is: MAM -100, the controller 20 is electrically connected to an external power supply, and the controller 20 is respectively connected to the operation panel 19, the photosynthesis lamp 17, the water quality detector 6, the exhaust pump 4, the sound and light alarm 8, The temperature sensor 16, the electric heating tube 14 and the cooling plate 15 are all electrically connected, and the water quality detector 6 is electrically connected with a detection probe 7, wherein the model of the photosynthesis lamp 17 is: 8015; the model of the exhaust pump 4 is: 4A12B25R37; The model of the temperature sensor 16 is: DS18B20; the model of the electric heating tube 14 is: CH-34; the model of the cooling plate 15 is: TE.

When the utility model is used, the tissue culture box is first placed in a sterile environment, and the culture medium is added into the incubator body 1 through the culture medium adding port 11, so that the culture medium does not pass through the temperature controller 16 and the refrigeration sheet. 15 and the end of the detection probe 7 that is far away from the water quality detector 6, then open the box cover 24, inoculate different plant tissues or cells in different culture chambers under aseptic conditions, and cover the box cover 24. It is assembled on the incubator body 1, and then the incubator body 1 and the box cover 24 are sealed and buckled through the lock. After the controller 20 is electrically connected with the power supply, the photosynthesis lamp 17, the water quality detector 6, and the exhaust pump 4 are turned on. , acousto-optic alarm 8, temperature sensor 16, electric heating tube 14 and control switch of refrigerating piece 15, adjust the temperature and illumination of the incubator body 1 and monitor the culture liquid through the water quality detector 6 and the detection probe 7, when the sound When the light alarm 8 gives an alarm, the valve on the liquid discharge port 9 is opened, and after the metamorphic culture medium flows out from the liquid discharge port 9, the valve on the liquid discharge port 9 is closed, and then the medium is added from the culture liquid addition port 11. culture medium and continue to cultivate.

Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present utility model fall within the scope of the claims of the present utility model and their equivalents, the present utility model is also intended to include these modifications and variations.

Claims (9)

1. An integrated plant tissue culture device, characterized in that it comprises an incubator body (1), a box cover (24) and a base (2), and the top of the incubator body (1) is detachably connected with the a box cover (24), the bottom of the incubator body (1) is provided with the base (2); An aeration tube (3) is detachably connected to the inner and lower part of the incubator body (1), and one end of the aeration tube (3) penetrates the side wall of the incubator body (1) and extends outward, and the end An exhaust pump (4) is connected to the outside of the incubator body, a plurality of exhaust holes are opened on the aeration pipe (3) located in the incubator body (1), and the side wall of the incubator body (1) is also provided with There is a liquid discharge port (9), the liquid discharge port (9) is located below the aeration pipe (3), and the incubator body (1) is located above the aeration pipe (3) and is also provided with a A first perforated plate (5), a water quality detector (6) is also provided in the incubator body (1) above the first perforated plate (5), and a water quality detector (6) is provided at the bottom There is a detection probe (7) in a vertical direction; An acousto-optic alarm (8) is provided on the outer wall of the incubator body (1), and a gas discharge port (10) and a culture solution adding port (11) are also provided on the side wall of the incubator body (1). ; Valves are provided on both the liquid discharge port (9) and the culture liquid addition port (11), and the gas discharge port (10), the culture liquid addition port (11) and the incubator body (11) are provided with valves. Microporous membrane filters (12) are provided in the aeration pipes (3) outside 1). 2 . An integrated plant tissue culture device according to claim 1 , wherein a transverse plate ( 13 ) is also provided in the incubator body ( 1 ) below the drain outlet ( 9 ). 3 . , a plurality of electric heating tubes (14) are arranged side by side at the bottom of the horizontal plate (13), a photosynthesis lamp (17) is arranged on the inner top of the box cover (24), and the inner side wall of the incubator body (1) A cooling sheet (15) and a temperature sensor (16) are also arranged thereon, and an operation panel (19) and a controller (20) are also arranged on the outer side wall of the incubator body (1). 3. An integrated plant tissue culture device according to claim 1, characterized in that, the outer top of the incubator body (1) is also provided with a cleaning box (25) for storing disinfectant, the cleaning One end of a circulation pipe (26) is connected to the box (25), and the other end of the circulation pipe (26) penetrates through the top of the incubator body (1) and is connected to the inner top of the incubator body (1). The sprinkler head (27) is connected through, the circulation pipe (26) is also provided with a valve, and the cleaning box (25) is provided with a disinfectant adding port (28). 4. An integrated plant tissue culture device according to claim 2, wherein the controller (20) is electrically connected to an external power supply, and the controller (20) is respectively connected to the operation panel (20). 19), the water quality detector (6), the exhaust pump (4), the sound and light alarm (8), the temperature sensor (16), the electric heating pipe (14), the refrigeration sheet (15), the photosynthesis lamp (17) Equal electrical connection. 5. An integrated plant tissue culture device according to claim 1, characterized in that a plurality of hanging rings (18) are arranged side by side on two opposite inner side walls of the incubator body (1), and the aeration The tubes (3) are arranged in an arcuate shape and are detachably connected with a plurality of the hanging rings (18), and the end of the aeration tube (3) extending into the incubator body (1) is fixedly arranged in the incubator on the inner side wall of the body (1). 6. An integrated plant tissue culture device according to claim 1, wherein the top of the first perforated plate (5) is divided into Several cultivation rooms. 7. An integrated plant tissue culture device according to claim 6, characterized in that the aperture of the sieve holes on the first perforated plate (5) is greater than 625 meshes, and the second perforated plate (21 ) and the sieve diameter of the first perforated plate (5) are the same. 8 . The integrated plant tissue culture device according to claim 1 , wherein an observation port ( 22 ) is further opened on the side wall of the incubator body ( 1 ), and an observation port ( 22 ) is provided in the observation port ( 22 ). Embedded with clear glass. 9 . The integrated plant tissue culture device according to claim 1 , wherein a universal wheel ( 23 ) is provided at the bottom of the base ( 2 ). 10 .

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