CN219032152U - Carbon dioxide incubator - Google Patents

Abstract

The carbon dioxide incubator comprises a box body, wherein the box body comprises a culture cavity, an evaporation cavity and a disinfection cavity, the culture cavity is arranged above the evaporation cavity, the disinfection cavity is arranged on the same side of the culture cavity and the evaporation cavity, a plurality of partition boards are arranged in the culture cavity, a sub-cavity is formed between every two adjacent partition boards, each sub-cavity is provided with an air outlet pipe, the air outlet pipes are arranged on the same side of the culture cavity, each air outlet pipe is communicated with an air inlet of the disinfection cavity, an air inlet channel is arranged on the other side of the culture cavity opposite to the air outlet pipe, the sub-cavity and the evaporation cavity are communicated with the air inlet channel, an air outlet of the disinfection cavity is communicated with the evaporation cavity, and a gas circulation device is arranged in the disinfection cavity; the utility model establishes the airflow circulation route of the evaporating cavity, the culturing cavity and the sterilizing cavity by utilizing the air circulation device, the evaporating cavity maintains stable humidity by natural evaporation, different sub-cavities use different air outlet pipes, and each air outlet pipe respectively guides the airflows in different sub-cavities into the sterilizing cavity for sterilization.

Description

Carbon dioxide incubator

Technical Field

The utility model relates to the technical field of incubators, in particular to a carbon dioxide incubator.

Background

The carbon dioxide incubator is a device for culturing cells/tissues in vitro by simulating and forming a growth environment similar to cells/tissues in a living body in the incubator, and the carbon dioxide incubator strictly controls the temperature and humidity of the environment in the incubator, such as a stable CO2 level (5%), a stable temperature (37 ℃) and a high relative humidity (95%), so that the success rate and the efficiency of culturing the biological cells, tissues and the like are improved.

The existing partial carbon dioxide incubator lacks air flow circulation, when carbon dioxide is filled into the carbon dioxide incubator, carbon dioxide is easily accumulated at the bottom of the carbon dioxide incubator because the density of the carbon dioxide is greater than that of the air, so that the uniformity of the concentration of the carbon dioxide in the carbon dioxide incubator is poor, and meanwhile, the effect of gas conduction temperature and moisture in the incubator is poor because of the lack of air flow, so that the temperature and humidity distribution in the carbon dioxide incubator is uneven.

Second, if different cells are cultured simultaneously in a carbon dioxide incubator, the cells, bacteria or microorganisms in different dishes may spread in the air, causing the problem of cross contamination of different samples during the culturing process, and thus a carbon dioxide incubator capable of improving the circulation of air flow in the incubator and reducing the risk of cross contamination of different samples is needed.

Disclosure of Invention

The utility model aims to solve the problems existing in the prior art and provides a carbon dioxide incubator.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a carbon dioxide incubator, includes the box, the box includes cultivates chamber, evaporating chamber and disinfection chamber, it is in to cultivate the chamber setting evaporating chamber top, the disinfection chamber sets up cultivate the chamber with the homonymy of evaporating chamber, cultivate the intracavity and be equipped with a plurality of baffles, adjacent constitute the subchamber between the baffle, every the subchamber all is equipped with out the tuber pipe, it all sets up to go out the tuber pipe cultivate the homonymy of chamber, every go out the tuber pipe all with the air inlet intercommunication of disinfection chamber, cultivate the chamber with the opposite side that goes out the tuber pipe is provided with the air inlet duct, the subchamber with the evaporating chamber all with the air inlet duct intercommunication, the gas outlet of disinfection chamber with evaporating chamber intercommunication, the disinfection intracavity is equipped with gas circulation device.

According to the utility model, the gas circulation device is utilized to establish the gas circulation route of the evaporation cavity, the culture cavity and the disinfection cavity, carbon dioxide is uniformly distributed in the culture box through gas circulation, and the evaporation cavity can supplement water consumed in the culture cavity, so that the condition of nonuniform temperature and humidity distribution in the carbon dioxide culture box is improved;

the culture cavity is divided into different subchambers by the partition plates, the different subchambers use different air outlet pipes so as to prevent air flow carrying pollutants passing through the subchambers at the lower part from entering the subchambers at the upper part, and all the air outlet pipes guide the air flow passing through the culture cavity into the disinfection cavity for disinfection, so that the air flow carrying pollutants are prevented from participating in the next round of air flow circulation.

Preferably, the air circulation device adopts an air pump, and the air pump is arranged at an air inlet or an air outlet of the disinfection cavity.

Preferably, the air circulation device adopts a guide fan, the guide fan comprises an air inlet guide fan and an air outlet guide fan, and the air inlet guide fan and the air outlet guide fan are respectively arranged at the air inlet and the air outlet of the disinfection cavity.

The air pump or the guide fan can suck air flow into the air inlet of the disinfection cavity and send the air flow out of the air outlet of the disinfection cavity, so that the air flow can pass through the disinfection cavity for disinfection and can circularly flow in the box body.

Preferably, the front and back of cultivateing the chamber all is provided with the hot plate, the hot plate is inlayed respectively on the wall of box and the sealing door, it is equipped with a plurality of supports to cultivate the intracavity, the baffle erects on the support, the baffle top surface is provided with the recess of placing the culture dish and the support of placing the test tube.

The heating plate around the culture cavity makes the temperature distribution in the culture cavity more even, when the test tube that needs to put into the incubator highly is higher than the height of subchamber, the baffle can be taken off from on the support in order to merge upper and lower adjacent subchamber to put into the test tube.

Preferably, the disinfection intracavity is equipped with ultraviolet ray degassing unit and from top to bottom sets gradually a plurality of deep bead, the vent has all been opened to one side of deep bead, the top in disinfection chamber is equipped with the carbon dioxide air inlet, be equipped with HEPA high efficiency filter screen layer in the carbon dioxide air inlet.

The disinfection chamber utilizes ultraviolet ray degassing unit is to flowing through the air current in disinfection chamber disinfects, the deep bead can force the air current to carry out tortuous motion in disinfection intracavity, increase the air current in disinfection intracavity the dwell time to better purification flows through the air current in disinfection chamber, the carbon dioxide air inlet can be connected with the carbon dioxide gas cylinder, the high-efficient filter screen layer of HEPA can filter bacterium and dust granule in the carbon dioxide gas.

Preferably, a water tank and an evaporation air duct are arranged in the evaporation cavity, the evaporation air duct is positioned above the water tank, the top of the water tank is provided with an evaporation tray, and a plurality of evaporation holes are formed in the evaporation tray.

Humidity, the degree of drying of air, the physical quantity of how much moisture contained in the air, because the culture in the culture chamber can consume moisture when cultivateing, leads to the moisture in the air to reduce, and the temperature often is in 30 to 40 in the incubator, when cultivateing the cell in the incubator, heats and can cause the culture medium to evaporate the water loss fast, consequently needs the water natural evaporation in the water tank, makes the humidity in the culture chamber remains higher level throughout, avoids the low humidity to lead to the culture medium to lose water too much.

Preferably, the box body is connected with the sealing door through a hinge, the sealing door is opposite to the culture cavity and the evaporation cavity, and a ventilation valve is arranged on the sealing door.

By opening the sealing door to take and place the sample in the culture cavity and the water tank in the evaporation cavity, when the pressure difference exists between the air pressure in the tank body and the external air pressure, the air pressure inside and outside the tank body can be balanced by using the air vent valve in order to conveniently open the sealing door.

Preferably, a carbon dioxide concentration sensor, a humidity sensor and a temperature sensor are arranged in the subchamber of the culture cavity far away from the evaporation cavity.

The humidity in the subchamber closest to the evaporation chamber is influenced by the evaporation chamber to the greatest extent, so that each sensor is far away from the evaporation chamber to avoid the influence of the evaporation chamber on each sensor, the opening and closing of a carbon dioxide air inlet are determined according to the carbon dioxide concentration measured by the carbon dioxide concentration sensor, and whether the heating plates around the culture chamber are opened or not is determined according to the temperature measured by the temperature sensor.

Compared with the prior art, the utility model has the beneficial effects that:

(1) According to the utility model, the gas circulation route of the evaporation cavity, the culture cavity and the disinfection cavity is established by using the gas circulation device, carbon dioxide is uniformly distributed in the culture cavity by gas circulation, and the evaporation cavity can supplement water consumed in the culture cavity, so that the condition of nonuniform temperature and humidity distribution in the carbon dioxide culture cavity is improved, and the temperature distribution in the culture cavity is more uniform by the heating plates in front of and behind the culture cavity.

(2) The culture cavity is divided into different subchambers by the partition plates, and the different subchambers use different air outlet pipes so as to prevent air flow carrying pollutants passing through the subchambers at the lower part from entering the subchambers at the upper part, and all the air outlet pipes guide the air flow passing through the culture cavity into the disinfection cavity for disinfection, so that the air flow carrying pollutants are prevented from participating in the circulation of the air flow of the next round.

(3) The disinfection cavity of the utility model utilizes the ultraviolet disinfection device to disinfect the air flow flowing through the disinfection cavity, the wind shield can force the air flow to perform zigzag movement in the disinfection cavity, and the residence time of the air flow in the disinfection cavity is increased, so that the air flow flowing through the disinfection cavity is better purified.

Drawings

FIG. 1 is a schematic diagram of the structure of the present utility model;

FIG. 2 is a schematic cross-sectional view of an embodiment of the present utility model with the sealing door removed;

FIG. 3 is a schematic diagram showing the positional relationship of air outlet pipes on the side of a culture chamber according to an embodiment of the utility model;

FIG. 4 is a schematic cross-sectional view of a third embodiment of the present utility model with the sealing door removed;

FIG. 5 is a schematic cross-sectional view of a fourth embodiment of the present utility model with the sealing door removed;

in the figure: 1. a culture chamber; 101. a partition plate; 102. an air outlet pipe; 103. an air inlet duct; 2. a disinfection chamber; 201. a wind deflector; 202. an ultraviolet lamp tube; 203. an ultraviolet soft lamp band; 3. an evaporation chamber; 301. an evaporation air duct; 302. a water tank; 4. an air pump; 5. sealing the door; 501. a seal ring; 502. a vent valve; 6. a heating plate; 7. a carbon dioxide inlet; 8. a water collection tank; 9. an air outlet guide fan; 10. an air inlet guide fan; 11. and a cover plate.

Detailed Description

The following description of the embodiments of the present utility model will be made more apparent and fully hereinafter with reference to the accompanying drawings, in which some, but not all embodiments of the utility model are shown. All other embodiments, which can be made by one of ordinary skill in the art without undue burden on the person of ordinary skill in the art based on embodiments of the present utility model, are within the scope of the present utility model.

In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "middle", "upper", "lower", "left", "right", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or element to be referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present utility model.

As shown in fig. 1 to 3, the specific scheme of the first embodiment is as follows: the utility model provides a carbon dioxide incubator, the power distribution box comprises a box body, the box includes cultivates chamber 1, evaporating chamber 3 and disinfection chamber 2, cultivate chamber 1 setting in evaporating chamber 3 top, disinfection chamber 2 sets up the homonymy at cultivate chamber 1 and evaporating chamber 3, cultivate intracavity 1 and be equipped with a plurality of baffles 101, constitute the subchamber between the adjacent baffle 101, every subchamber all is equipped with out tuber pipe 102, it all sets up the homonymy at cultivate chamber 1 to go out tuber pipe 102, every goes out tuber pipe 102 all communicates with the air inlet of disinfection chamber 2, cultivate chamber 1 at opposite side relative with play tuber pipe 102 is provided with air inlet channel 103, subchamber and evaporating chamber 3 all communicate with air inlet channel 103, the gas outlet and the evaporating chamber 3 intercommunication of disinfection chamber 2 are equipped with gas circulation device in the disinfection chamber 2.

Specifically, the airflow circulation path in the case is as follows:

the air circulation device blows air flow through the evaporation air duct 301 of the evaporation cavity 3, the air flow flows into the air inlet duct 103 through the evaporation air duct 301, the air flow enters each subchamber through the air inlet duct 103, the air flow in each subchamber flows out of different air outlet pipes 102 and is converged into the disinfection cavity 2, and the air flow bypasses each wind shield 201 in the disinfection cavity 2 to reenter the evaporation cavity 3 under the action of the air circulation device, so that a complete air flow circulation is completed.

Specifically, the air flow can flow out from the air outlet pipe 102 of each subchamber after entering the subchamber, and the air outlet pipe 102 of each subchamber is directly communicated with the disinfection cavity 2, so that pollutants such as bacteria in the subchamber below the culture cavity 1 are prevented from being brought to the subchamber above the culture cavity 1 by the air flow, and a polluted culture dish or test tube in one subchamber is prevented from polluting the culture dish or test tube in the whole culture cavity 1.

Specifically, be equipped with the heat preservation in the wall of box, all be provided with hot plate 6 around cultivateing chamber 1, hot plate 6 inlay respectively on the wall of box and sealing door 5, cultivate chamber 1 and be equipped with a plurality of supports, baffle 101 erects on the support, and baffle 101 top surface is provided with the recess of placing the culture dish and places the support of test tube.

Specifically, be equipped with ultraviolet ray disinfection device and from top to bottom two to four deep bead 201 that set gradually in disinfection chamber 2, the vent has all been opened to one side of deep bead 201, and the vent of upper and lower adjacent deep bead 201 staggers the setting, makes the air current pass disinfection chamber 2 with tortuous route, and the top of disinfection chamber 2 is equipped with carbon dioxide air inlet 7, is connected with the relief pressure valve on the carbon dioxide air inlet 7, and carbon dioxide air inlet 7 is connected with the carbon dioxide gas cylinder through the relief pressure valve, is equipped with HEPA high-efficient filter screen layer in the carbon dioxide air inlet 7.

Specifically, the water collecting tank 8 is arranged below the disinfection cavity 2, the disinfection cavity 2 and the water collecting tank 8 are communicated through a water outlet pipe arranged at the bottom of the disinfection cavity 2, and condensed water appearing on each baffle plate in the disinfection cavity 2 flows into the water collecting tank 8 through the water outlet pipe.

Specifically, the air circulation device adopts the air pump 4, and the air pump 4 sets up in the gas outlet department of disinfection chamber 2, and the air pump 4 is located the top of water catch bowl 8, and the air inlet of air pump 4 is higher than the bottom of disinfection chamber 2, avoids comdenstion water inflow air pump 4, and the gas outlet and the evaporation wind channel 301 intercommunication of air pump 4.

Specifically, the cover plate 11 is arranged at the position of the tank body, which is opposite to the water collecting tank 8, and the cover plate 11 can be opened to take out the water collecting tank 8 so as to clean accumulated water in the water collecting tank 8.

Specifically, the ultraviolet disinfection device adopts an ultraviolet lamp tube 202 arranged in the center, and the ultraviolet lamp tube 202 penetrates through each wind shield 201 to be connected with the top and the bottom of the disinfection cavity 2.

Specifically, a water tank 302 and an evaporation air duct 301 are arranged in the evaporation cavity 3, the water tank 302 is detachably fixed in the evaporation cavity 3 through a bracket, sterile distilled water is filled in the water tank 302, the evaporation air duct 301 is positioned above the water tank 302, the top of the water tank 302 is provided with an evaporation tray, a plurality of evaporation holes are formed in the evaporation tray, and two ends of the evaporation air duct 301 are respectively communicated with the air inlet duct 103 and the disinfection cavity 2.

Specifically, the box passes through hinged joint with sealing door 5, and sealing door 5 just sets up culture chamber 1 and evaporating chamber 3, and the round sealing washer 501 has been encircleed on the sealing door 5 outside, is equipped with breather valve 502 on the sealing door 5, and breather valve 502 is for establishing respectively at the inside and outside two overlapping turntables of sealing door 5, accomplishes the ventilation when the air vent on two turntables is relative, and breather valve 502 manual control is opened, culture chamber 1 and evaporating chamber 3 direct exposure in the external world after opening sealing door 5, and disinfection chamber 2 and fan do not contact with external world directly.

Specifically, a carbon dioxide concentration sensor, a humidity sensor and a temperature sensor are arranged in the uppermost subchamber of the culture cavity 1, the carbon dioxide concentration sensor monitors the concentration of CO2 by adopting a heat conduction sensor or an Infrared (IR) sensor, and as carbon dioxide is easy to precipitate at the bottom of the culture cavity 1 and the humidity and the temperature of the bottom of the culture cavity 1 tend to be highest due to the existence of the evaporation cavity 3, the lowest value in the culture cavity 1 can be measured by arranging each sensor at the uppermost part of the culture cavity 1, so that the concentration, the temperature or the humidity of the carbon dioxide is prevented from being too low.

Specifically, the working principle of the carbon dioxide incubator is as follows:

the utility model can adopt two sterilization methods of high-temperature wet heat sterilization or high-temperature dry heat sterilization, wherein the high-temperature wet heat sterilization needs to add sterile distilled water into a water tank 302, close a sealing door 5, open a gas circulation device and all heating plates 6, monitor the conditions in the tank through a temperature sensor and a humidity sensor, and sterilize the whole incubator by utilizing the high temperature and high humidity environment at 90 ℃; the high-temperature dry heat sterilization only needs to directly open the gas circulation device and all heating plates 6, and the high-temperature environment of 120-140 ℃ is utilized to sterilize the whole incubator.

In the culturing process of the incubator, the standard temperature and the maximum temperature difference are set according to the standards of different cultured cells, each heating plate 6 is intermittently opened to maintain constant temperature, for example, the proper culturing temperature of fish, insects and amphibian cells is 25-28 ℃, the proper culturing temperature of mammal cells is 35-37 ℃, and the transformed cells are suitable for growing at the temperature higher than 40 ℃; the water in the water tank 302 is naturally evaporated through the evaporating pan at the ambient temperature in the incubator, so that the humidity environment in the incubator meets the cell culture requirement; and (3) opening a pressure reducing valve to charge carbon dioxide into the incubator, and intermittently opening the pressure reducing valve to maintain the carbon dioxide concentration when the carbon dioxide concentration in the incubator meets the cell culture standard.

Example 2: this embodiment 2 differs from embodiment 1 in that:

specifically, the gas circulation device adopts the air pump 4, and air pump 4 sets up in the air inlet department of disinfection chamber 2, and air pump 4 air inlet and each tuber pipe 102 intercommunication, air pump 4 gas outlet and disinfection chamber 2 intercommunication, the gas outlet of disinfection chamber 2 pass water catch bowl 8 and directly communicate with evaporating chamber 3 through the pipeline of giving vent to anger, and the import of pipeline of giving vent to anger is higher than the bottom of disinfection chamber 2, avoids the comdenstion water to flow into the pipeline of giving vent to anger.

Example 3: as shown in fig. 4, this embodiment 3 differs from embodiment 1 in that:

specifically, the ultraviolet sterilizing apparatus employs an ultraviolet soft lamp band 203, and the ultraviolet soft lamp band 203 is disposed around the cavity wall of the sterilizing cavity 2 to uniformly irradiate the inside of the entire sterilizing cavity 2.

Example 4: as shown in fig. 5, this embodiment 4 differs from embodiment 1 in that:

specifically, the gas circulation device adopts a guide fan, the guide fan comprises an air inlet guide fan 10 and an air outlet guide fan 9, and the air inlet guide fan 10 and the air outlet guide fan 9 are respectively arranged at the air inlet and the air outlet of the disinfection cavity 2.

Specifically, the disinfection chamber 2 bottom is equipped with a plurality of ventilation holes, and disinfection chamber 2 passes through ventilation hole and water catch bowl 8 intercommunication, and gas and comdenstion water all can pass through the ventilation hole.

Specifically, the air inlet guide fan 10 is communicated with each air outlet pipe 102, the air outlet guide fan 9 is arranged between the evaporation cavity 3 and the water collecting tank 8, the air inlet guide fan 10 sucks air from the culture cavity 1 into the disinfection cavity 2 through each air outlet pipe 102, and the air outlet guide fan 9 withdraws the air from the upper part of the water collecting tank 8 and sends the air into the evaporation air duct 301.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims (8)

1. The utility model provides a carbon dioxide incubator, includes box, its characterized in that: the box includes cultivates chamber, evaporating chamber and disinfection chamber, cultivate the chamber setting and be in evaporating chamber top, the disinfection chamber sets up cultivate the chamber with the homonymy in evaporating chamber, cultivate the intracavity and be equipped with a plurality of baffles, adjacent constitute the subchamber between the baffle, every the subchamber all is equipped with out the tuber pipe, it all sets up to go out the tuber pipe cultivate the homonymy in chamber, every go out the tuber pipe all with the air inlet intercommunication in disinfection chamber, cultivate the chamber with the opposite side that goes out the tuber pipe is provided with the air inlet channel, the subchamber with the evaporating chamber all with the air inlet channel intercommunication, the gas outlet in disinfection chamber with evaporating chamber intercommunication, be equipped with gas circulation device in the disinfection chamber.

2. A carbon dioxide incubator according to claim 1, wherein: the gas circulation device adopts an air pump, and the air pump is arranged at the air inlet or the air outlet of the disinfection cavity.

3. A carbon dioxide incubator according to claim 1, wherein: the air circulation device adopts a guide fan, the guide fan comprises an air inlet guide fan and an air outlet guide fan, and the air inlet guide fan and the air outlet guide fan are respectively arranged at the air inlet and the air outlet of the disinfection cavity.

4. A carbon dioxide incubator according to claim 1, wherein: the culture chamber is provided with the hot plate around, the hot plate is inlayed respectively on the case wall of box and the sealing door, be equipped with a plurality of supports in the culture chamber, the baffle erects on the support, the baffle top surface is provided with the recess of placing the culture dish and places the support of test tube.

5. A carbon dioxide incubator according to claim 1, wherein: the disinfection chamber is equipped with ultraviolet ray disinfection device and from top to bottom sets gradually a plurality of deep bead, the vent has all been opened to one side of deep bead, the top in disinfection chamber is equipped with the carbon dioxide air inlet, be equipped with HEPA high-efficient filter screen layer in the carbon dioxide air inlet.

6. A carbon dioxide incubator according to claim 1, wherein: the evaporation cavity is internally provided with a water tank and an evaporation air duct, the evaporation air duct is positioned above the water tank, the top of the water tank is provided with an evaporation tray, and a plurality of evaporation holes are formed in the evaporation tray.

7. The carbon dioxide incubator of claim 4, wherein: the box body is connected with the sealing door through a hinge, the sealing door is opposite to the culture cavity and the evaporation cavity, and a ventilation valve is arranged on the sealing door.

8. A carbon dioxide incubator according to claim 1, wherein: and a carbon dioxide concentration sensor, a humidity sensor and a temperature sensor are arranged in the subchamber of the culture cavity far away from the evaporation cavity.

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