CN210394377U

CN210394377U - Anaerobe culture system

Info

Publication number: CN210394377U
Application number: CN201920941548.4U
Authority: CN
Inventors: 冯艳忠; 何流琴; 冯哲; 李凤兰; 吴建; 杨秀梅
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-06-21
Filing date: 2019-06-21
Publication date: 2020-04-24
Anticipated expiration: 2029-06-21

Abstract

An anaerobic bacteria culture system includes a tank that is vertically mounted to a base. The tank body comprises a shell, a heat preservation layer, a temperature adjusting component and an inner container, wherein the shell is arranged on the outermost side of the tank body, the heat preservation layer and the temperature adjusting component are located between the shell and the inner container, and the inner container limits a space for culturing anaerobic bacteria. The tank body is also provided with a plurality of interfaces which can be communicated with the outside and are used for introducing materials required for anaerobic bacteria culture, discharging the discharge to the outside and/or connecting external equipment. The anaerobic bacteria culture system also comprises a liquid circulation reflux device which is composed of pipelines arranged inside and outside the tank body.

Description

Anaerobe culture system

Technical Field

The utility model belongs to the technical field of anaerobe is cultivateed, concretely relates to anaerobe cultivation system.

Background

Anaerobic bacteria (anaerobes) are a group of bacteria that grow better under Anaerobic conditions than in aerobic environments, but cannot grow on the surface of solid media at air (18% oxygen) and/or 10% carbon dioxide concentrations, and they lack an integral metabolic enzyme system, whose energy metabolism proceeds anaerobically. The anaerobic bacteria have wide application prospect and are widely applied to the aspects of metallurgical industry, sewage treatment, disease detection and treatment and the like. However, most of anaerobic bacteria belong to non-culturable bacteria, which causes inconvenience in development and utilization.

At present, methods, devices and apparatuses for anaerobic bacteria culture are many, including Hungate rolling tube technology, anaerobic glove box technology, anaerobic tank and anaerobic bag culture method, etc. However, some of these anaerobic culture devices are expensive, and the subsequent maintenance and material consumption costs are very high, and the operation is also complicated. Therefore, the research on anaerobic bacteria in laboratories with limited conditions is restricted to a certain extent.

Culturing methods requiring relatively low anaerobic conditions such as alkaline pyrogallol method, anaerobic tube method, and Pachyrhizus culture method. The methods are simple to operate and can quickly establish an anaerobic environment. However, anaerobic culture is inefficient due to poor oxygen removal. In addition, the reaction systems are easy to cause pollution, breed other bacteria and possibly produce other gases such as CO and CO₂And H₂O, etc., or consuming CO₂Thereby affecting the growth of the anaerobic bacteria and reducing the culture efficiency of the anaerobic bacteria.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an anaerobe culture system, it includes a jar body, jar body is installed in the basement with erectting. The tank body comprises a shell, a heat preservation layer, a temperature adjusting component and an inner container, wherein the shell is arranged on the outermost side of the tank body, the heat preservation layer and the temperature adjusting component are located between the shell and the inner container, and the inner container limits a space for culturing anaerobic bacteria. The tank body is provided with a plurality of interfaces which can be communicated with the outside and are used for introducing materials required for anaerobic bacteria culture, discharging the discharge to the outside and/or connecting external equipment.

Preferably, the temperature adjustment member is a spiral temperature adjustment member located between the top and bottom of the tank and surrounding the side of the inner container.

Preferably, the temperature regulating component is connected with an external temperature regulating liquid storage container through a temperature regulating liquid input pipeline and a temperature regulating liquid output pipeline to form a temperature regulating liquid circulating flow loop, the temperature regulating liquid input pipeline is connected with a pipeline pump, and the pipeline pump is used for driving temperature regulating liquid to flow into the temperature regulating component from the temperature regulating liquid storage container through the temperature regulating liquid input pipeline and then flow back to the temperature regulating liquid storage container through the temperature regulating liquid output pipeline.

Preferably, a first pipeline is arranged outside the tank body, and a second pipeline and a circular pipeline are arranged inside the inner container. The upper end part of the second pipeline is connected with the first pipeline, the lower end part of the second pipeline is connected with the circular pipeline, a gap is reserved between the circular pipeline and the bottom of the inner container, and a plurality of orifices which are opened towards the bottom of the inner container are arranged on the circular pipeline. A third pipeline is arranged below the bottom of the tank body, one end of the third pipeline penetrates through the bottom of the tank body and is communicated with the inner container, the other end of the third pipeline is provided with an opening forming a liquid outlet, and the first pipeline is communicated with the third pipeline between two end parts of the third pipeline. The first pipeline, the second pipeline, the circular pipeline and the third pipeline are constructed to form a liquid circulation loop, the first pipeline is connected with a stainless steel pump, and the stainless steel pump is used for driving liquid to flow from the first pipeline to the second pipeline, the circular pipeline and the third pipeline in sequence.

Preferably, the first pipeline comprises a branch pipeline positioned at the downstream of the stainless steel pump and is configured to penetrate through the side wall of the tank body to be communicated with the inner container, and a valve is arranged on the branch pipeline and is used for controlling the communication between the branch pipeline and the inner container.

Preferably, the first conduit comprises a bypass conduit upstream of the stainless steel pump, the bypass conduit being configured to be connectable to an external liquid container and to drive liquid in the liquid container along the bypass conduit into the first conduit by the stainless steel pump.

Preferably, the first pipe is connected to an electrically heated steam generator.

Preferably, the plurality of interfaces includes: a manhole/feeding port, a nutrient input port, a water inlet, an air outlet and a waste gas outlet which are arranged at the top of the tank body, and a material outlet which is arranged at the bottom of the tank body.

Preferably, the air outlet is connected with a vacuum pump, and a valve is arranged on a pipeline between the air outlet and the vacuum pump.

Preferably, the insulation layer is a polyester insulation layer.

The utility model provides an anaerobe culture system utilizes various pipelines to form the liquid circulation return circuit that communicates with the internal culture phase of jar for the liquid state is cultivateed the mixture and can be obtained abundant stirring and mix, and then improves the quality and the efficiency that the thallus was cultivateed. On the other hand, the liquid circulation loop is connected with the electric heating steam generator, and the liquid circulation loop and the tank body are sterilized at high temperature through high-temperature steam generated by the electric heating steam generator, so that a pollution source can be prevented or reduced.

Furthermore, the utility model discloses an anaerobe culture system still can ensure the environment of the internal anaerobic of jar or hypoxemia through corresponding interface connection vacuum pump and suitable gas source to do benefit to the growth of anaerobe, and then cultivate high-quality anaerobe product.

The temperature regulation component arranged around the tank inner container can help maintain the environmental temperature in the tank body at a constant temperature, can ensure the advantageous growth of required anaerobic bacteria, and further improves the culture efficiency and the quality of cultures.

In addition, the nutrient substance input port is arranged and is connected to an external nutrient substance storage container through the rubber tube, so that the pH value can be adjusted and/or nutrient substances can be supplemented in time in the fermentation process, the stable and advantageous growth of required anaerobic bacteria is ensured, and the culture efficiency and the quality of a culture are improved.

The utility model provides an anaerobe culture system is applicable to the cultivation of single anaerobism bacterial strain and compound anaerobism fungus crowd.

Drawings

FIG. 1 is a schematic sectional view showing the structure of the anaerobic culture system of the present invention.

Detailed Description

The technical solution of the present invention will be described in detail with reference to the following embodiments.

Fig. 1 shows a schematic structural diagram of an anaerobic bacteria culture system provided by the present invention. As shown in FIG. 1, the anaerobic cultivation system includes a tank 1, wherein the tank 1 is supported on the ground vertically by legs 2 at the bottom thereof, and the bottom of the tank 1 is spaced from the ground. The tank body 1 is in a cylindrical shape with an upper end enclosure and a lower end enclosure. The upper end enclosure and the end enclosure of the tank body 1 can be fixedly and hermetically connected with the side part of the tank body into a whole in a welding mode and the like. The upper end enclosure of the tank body 1 forms the top of the tank body 1, and the lower end enclosure forms the bottom of the tank body 1.

The top of jar body 1 is provided with a plurality of interfaces, mainly includes: a manhole/batch inlet 16, a nutrient input 17, a water inlet 18, an air inlet 19, an air outlet 20 and an exhaust gas outlet 21. The positions of these openings are not particularly limited, and an appropriate and reasonable layout can be made as necessary.

The inner container of the tank 1 can be cleaned manually and the material can be discharged through the manhole/inlet 16. The nutrient input port 17 is connected to an external nutrient storage container 23 through a hose 22, and nutrients are timely supplied to the tank 1 during the anaerobic culture process based on the need such as pH adjustment. The rubber tube 22 is provided with a valve for controlling the communication between the nutrient storage container 23 and the inner container of the tank body 1. The water inlet 18 is connected with an external water source such as a tap water pipe on one hand and is connected with a cleaning ball (not shown) in the tank body 1 on the other hand, and the communication between the water inlet 18 and the external water source is controlled by a valve. The gas inlet 19 is used for conveying inert gas and CO to the inner container of the tank body 1₂And the gas is used for providing an anaerobic environment required by the culture of the anaerobic bacteria. The air outlet 20 is connected to an external vacuum pump 24, and air is discharged from the tank 1 by the vacuum pump 24. The exhaust gas outlet 21 is used for discharging the exhaust gas in the tank 1, and may be connected to an external exhaust gas filtering device. And valves are arranged on external pipelines connected with the interfaces to control the communication between the interfaces and the outside.

The bottom of the tank body 1 is provided with a material outlet 25 for discharging various materials. As shown in FIG. 1, the tank 1 is further provided with a sampling port 26, and the culture can be sampled through the sampling port 26 during the culture process, so that the growth state of the anaerobic bacteria can be monitored and/or analyzed in time. The position of the sampling port 26 is not limited to the position shown in fig. 1, and may be set at other suitable positions.

As shown in FIG. 1, the anaerobic bacteria culture system is further provided with a pH meter probe 27 for monitoring the pH of the culture, a liquid level meter 28 for monitoring the liquid level in the tank 1, and a temperature control probe 29 for monitoring the temperature of the culture in the tank 1.

The tank body 1 comprises a shell, a heat preservation layer, a temperature adjusting component 11 and an inner container from outside to inside in sequence. The inner space of the liner limits the space for culturing anaerobic bacteria. The temperature adjusting member 11 is disposed around the side of the inner container, preferably a spiral temperature adjusting member made of 304 stainless steel, and is fixed to the outer side of the inner container by welding so as to transmit heat to the inner container or absorb heat from the inner container, thereby maintaining the inner container and the anaerobic culture system therein at a desired temperature. The shell and the inner container are preferably made of 304 stainless steel, and the insulating layer is preferably polyester insulating layer.

The temperature adjusting component 11 is connected with the temperature adjusting liquid storage container 3 through the temperature adjusting liquid input pipeline 4 and the temperature adjusting liquid output pipeline 5 to form a temperature adjusting liquid circulating flow loop. The temperature regulating liquid input pipeline 4 is connected with a pipeline pump 6. As shown by the arrows in fig. 1, the temperature-adjusting liquid is driven by the pipe pump 6 from the lower end of the temperature-adjusting member 11 into the temperature-adjusting member 11 via the temperature-adjusting liquid input pipe 4, flows from the low position to the high position, and then flows back into the temperature-adjusting liquid storage container 3 via the temperature-adjusting liquid output pipe 5. Downstream of the pipe pump 6 in the direction of liquid flow, a valve is arranged for controlling the fluid communication between the tempering liquid storage container 3 and the tempering element 11. The temperature adjusting liquid contained in the temperature adjusting liquid storage container 3 is preferably water having a predetermined temperature.

The anaerobic culture system of the present application is provided with a liquid circulation loop device, which is substantially constituted by a pipe made of stainless steel. As shown in fig. 1, the liquid circulation circuit device generally includes a first pipe 7, a second pipe 8, an annular pipe 9, and a third pipe 10.

The first conduit 7 is located outside the tank 1 and the second conduit 8 is located inside the tank 1. The upper end of the first duct 7 is connected to the upper end of the second duct 8. The lower end of the second conduit 8 is connected to the ring conduit 9. The annular duct 9 is spaced from the bottom wall of the liner by a certain distance, and the annular duct 9 is provided with a plurality of orifices opening towards the bottom wall of the liner. The third pipeline 10 is positioned below the bottom of the tank body 1, the upper end part of the third pipeline penetrates through the bottom of the tank body 1 and is communicated with the inner container, the lower end part of the third pipeline is provided with an opening for forming a liquid discharge port, and the liquid discharge port can be used for discharging waste liquid in the tank body 1. The first pipe 7 is connected to the third pipe 10 between both ends of the third pipe 10.

The first pipeline 7 is connected with a stainless steel pump 12, and the stainless steel pump 12 drives the liquid to flow from the first pipeline 7 to the directions of the second pipeline 8, the circular pipeline 9 and the third pipeline 10 in sequence. A valve is provided downstream of the stainless steel pump 12 for controlling the communication between the first conduit 7 and the second conduit 8.

As shown in fig. 1, the first duct 7 is also provided with a first branch duct 13 and a second branch duct 14.

A first branch conduit 13 is located downstream of the stainless steel pump 12 and has one end extending through the side wall of the tank 1 into the liner so as to be in fluid communication therewith. The first branch pipe 13 is provided with a valve for controlling the communication with the inner container. The first branch pipe 13 is preferably provided to penetrate the side wall of the tank 1 at a position midway on the side of the tank 1.

A second branch conduit 14 is located upstream of the stainless steel pump 12 and may be connected to an external liquid storage container (not shown) so that liquid in the liquid storage container is pumped by the stainless steel pump 12 into the first conduit 7 and thence to the inner bladder of the tank 1. A valve is provided in the second branch conduit 14 for controlling its communication with the liquid storage container.

In the liquid circulation circuit device, when the first pipe 7, the second pipe 8, the circular pipe 9 and the third pipe 10 are controlled by the valves to form a circuit, the stainless steel pump 12 can draw out the liquid (for example, the anaerobic culture medium) in the inner container through the third pipe 10 to drive the liquid to flow into the first pipe 7. On one hand, the liquid in the first pipeline 7 flows back to the inner container from the middle position of the tank body through the first branch pipeline 13 by means of the inclined plane pipe in the middle of the tank body in a jet mode to push the liquid culture medium in the tank to horizontally rotate for 360 degrees, on the other hand, the liquid enters the circular pipeline 9 through the second pipeline 8, flows to the bottom wall of the inner container from the circular pipeline 9 towards a plurality of holes of the bottom wall opening of the inner container, flows upwards in the reverse direction after impacting the bottom wall of the inner container, and stirs the culture medium in the tank body 1, particularly the culture medium at the bottom without dead angles, so that the culture medium can be.

The first pipe 7 is also connected with an electric heating steam generator 15, and high-temperature steam generated by the electric heating steam generator 14 flows to the second pipe 8 and the circular ring pipe 9 through the first pipe 7 and then further enters the inner container of the tank body 1 for sterilizing and disinfecting the pipes and the tank body.

Although a few exemplary embodiments of the present invention have been shown and described, it would be appreciated by those skilled in the art that changes may be made in these exemplary embodiments without departing from the principles and spirit of the application, the scope of which is defined in the claims and their equivalents. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is chosen in order to best explain the principles of the embodiments, the practical application, or improvements made to the technology in the marketplace, or to enable others of ordinary skill in the art to understand the embodiments disclosed herein.

Claims

1. An anaerobe culture system, which comprises a tank body vertically installed on a substrate, and is characterized in that:

the tank body comprises a shell, a heat preservation layer, a temperature adjusting component and an inner container, the shell is arranged on the outermost side of the tank body, the heat preservation layer and the temperature adjusting component are positioned between the shell and the inner container, the inner container limits a space for culturing anaerobic bacteria,

the tank body is provided with a plurality of interfaces which can be communicated with the outside and are used for introducing materials required for anaerobic bacteria culture into the tank body, discharging the discharge to the outside and/or connecting external equipment.

2. The anaerobic culture system of claim 1, wherein: the temperature adjusting component is a spiral temperature adjusting component which is positioned between the top and the bottom of the tank body and is arranged around the side part of the inner container.

3. The anaerobic culture system according to claim 1 or 2, wherein:

the temperature adjusting component is connected with an external temperature adjusting liquid storage container through a temperature adjusting liquid input pipeline and a temperature adjusting liquid output pipeline to form a temperature adjusting liquid circulating flow loop, the temperature adjusting liquid input pipeline is connected with a pipeline pump, and the pipeline pump is used for driving temperature adjusting liquid to flow into the temperature adjusting component from the temperature adjusting liquid storage container through the temperature adjusting liquid input pipeline and then flow back to the temperature adjusting liquid storage container through the temperature adjusting liquid output pipeline.

4. The anaerobic culture system of claim 1, wherein:

a first pipeline is arranged outside the tank body, a second pipeline and a circular pipeline are arranged inside the inner container,

the upper end part of the second pipeline is connected with the first pipeline, the lower end part of the second pipeline is connected with the circular pipeline, a gap is reserved between the circular pipeline and the bottom of the inner container, a plurality of orifices which are opened towards the bottom of the inner container are arranged on the circular pipeline,

a third pipeline is arranged below the bottom of the tank body, one end of the third pipeline penetrates through the bottom of the tank body and is communicated with the inner container, the other end of the third pipeline is provided with an opening for forming a liquid outlet, the first pipeline is communicated with the third pipeline between the two end parts of the third pipeline,

the first pipeline, the second pipeline, the circular pipeline and the third pipeline are constructed to form a liquid circulation loop, the first pipeline is connected with a stainless steel pump, and the stainless steel pump is used for driving liquid to flow from the first pipeline to the second pipeline, the circular pipeline and the third pipeline in sequence.

5. The anaerobic culture system of claim 4, wherein: the first pipeline comprises a branch pipeline positioned at the downstream of the stainless steel pump, the branch pipeline is constructed to penetrate through the side wall of the tank body and communicated with the inner container, and a valve is arranged on the branch pipeline and used for controlling the communication between the branch pipeline and the inner container.

6. The anaerobic culture system of claim 4, wherein: the first conduit includes a bypass conduit upstream of the stainless steel pump, the bypass conduit configured to be connectable to an external liquid container and to drive liquid in the liquid container along the bypass conduit into the first conduit by the stainless steel pump.

7. The anaerobic culture system according to any one of claims 4 to 6, wherein: the first pipe is connected to an electrically heated steam generator.

8. The anaerobic culture system of claim 1, wherein:

the plurality of interfaces includes: a manhole/feeding port, a nutrient input port, a water inlet, an air outlet and a waste gas outlet which are arranged at the top of the tank body, and a material outlet which is arranged at the bottom of the tank body.

9. The anaerobic culture system of claim 8, wherein: the air outlet is connected with a vacuum pump, and a valve is arranged on a pipeline between the air outlet and the vacuum pump.

10. The anaerobic culture system of claim 1, wherein: the heat-insulating layer is a polyester heat-insulating layer.