CN110656043A - Microbial fermentation tank for producing liquid feed additive - Google Patents

Microbial fermentation tank for producing liquid feed additive Download PDF

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Publication number
CN110656043A
CN110656043A CN201810693352.8A CN201810693352A CN110656043A CN 110656043 A CN110656043 A CN 110656043A CN 201810693352 A CN201810693352 A CN 201810693352A CN 110656043 A CN110656043 A CN 110656043A
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fermentation tank
tank body
feed additive
liquid feed
pipe
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CN201810693352.8A
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Inventor
荆弘明
陈莹
舒忠梅
左亚尧
祝富强
王晓丹
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Deer Rock
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Deer Rock
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
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    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04Filters; Permeable or porous membranes or plates, e.g. dialysis
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    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
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    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20Degassing; Venting; Bubble traps
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    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/26Conditioning fluids entering or exiting the reaction vessel
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/02Means for regulation, monitoring, measurement or control, e.g. flow regulation of foam
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48Automatic or computerized control

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Abstract

The invention relates to the technical field of microbial fermentation, in particular to a microbial fermentation tank for producing a liquid feed additive. The invention improves the mixing uniformity of the zymophyte and the fermentation substrate, realizes the rapid culture of aerobic bacteria, has good fermentation effect, safety and sanitation, convenient operation, accurate regulation and control and easy automation, can be completely applied to occasions with large treatment capacity and large volume of fermentation tanks, and provides good material basis for the popularization and the application of the microbial feed additive.

Description

Microbial fermentation tank for producing liquid feed additive
Technical Field
The invention relates to the technical field of microbial fermentation, in particular to a microbial fermentation tank for producing a liquid feed additive.
Background
The fermentation tank is widely applied to the industries of pharmacy, fine chemical engineering, bioengineering and the like, and is a device for the growth, propagation and material reaction of microorganisms in the fermentation process. Microbial fermentation refers to a process of converting raw materials into products required by human beings through a specific metabolic pathway by using microorganisms under appropriate conditions. The microbial strain is widely applied to feed additives, and has important effects on improving the quality of the feed additives and improving the performance of the feed. In the fermentation process of the microbial strains, stable and appropriate fermentation environment needs to be provided, and the conditions of strain propagation and culture are met, so that the fermentation quality and efficiency of the microbial strains can be ensured. In the prior art, in the process of fermenting microbial strains, generally, the internal air permeability of a fermented product is poor, and the uniformity of strains and a fermentation substrate is poor, so that the fermentation period is long, the fermentation efficiency is low, the activity of the microbial strains obtained by fermentation is low, and the application and popularization of the microbial strains in feed additives are limited. When the existing aerobic strains are cultured, the adopted fermentation tank has single function and cannot adapt to the modern high-efficiency fermentation of the microbial strains, so that the prior art needs to be improved.
Based on the analysis, the improvement of the existing microbial strain fermentation technology used in the feed additive, and how to design a microbial fermentation tank matched with the new fermentation technology, becomes the problem to be solved currently.
Disclosure of Invention
The invention aims to provide a microbial fermentation tank for producing a liquid feed additive, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention adopts the following technical scheme:
microbial fermentation tank for producing liquid feed additive is characterized in that the fermentation tank comprises a fermentation tank body, a heating pipe, a steel cylinder sleeve, an outer heat-insulating layer, a motor, a sterile shaft seal, a defoamer, a stirring shaft, a stirrer, an air injection device, an air filter, a water valve, a viewing mirror, a digital display thermometer, a feeding port, a pressure gauge, a PH meter, a dissolved oxygen detector, an automatic control valve, an exhaust pipe, a sampling pipe, a temperature sensor, a cooling water inlet pipe, a cooling water outlet pipe, a discharging pipe, an air inlet pipe, a discharging pipe valve and a control switch valve.
Compared with the prior art, the invention has the beneficial effects that: the invention realizes the advantages of rapid culture of aerobic bacteria, good fermentation effect, safety, sanitation, convenient operation, accurate regulation and control, easy automation and high total efficiency. Can be completely suitable for occasions with large treatment capacity, large volume and large fermentation tanks, lays a good foundation for the scale production of microorganisms, and has wide market prospect and application prospect.
Drawings
The invention is further illustrated with reference to the figures and embodiments.
FIG. 1 is a schematic view of the structure of the present invention.
Detailed Description
Referring to fig. 1, through the description of the embodiments, the detailed implementation of the present invention, such as the interrelation between the related parts, the function and operation of the parts, the operation and usage method, etc., will be further described in detail to help the engineer in the field to understand the concept of the present invention more completely, accurately and deeply.
The invention relates to a microbial fermentation tank for producing a liquid feed additive, which structurally comprises a fermentation tank body 1, a heating pipe 2, a steel cylinder sleeve 3, an external heat-insulating layer 4, a motor 5, a sterile shaft seal 6, a defoamer 7, a stirring shaft 8, a stirrer 9, an air injection device 10, an air filter 11, a water valve 12, a viewing mirror 13, a digital display thermometer 14, a feeding hole 15, a pressure gauge 16, a PH meter 17, a dissolved oxygen detector 18, an automatic control valve 19, an exhaust pipe 20, a sampling pipe 21, a temperature sensor 22, a cooling water inlet pipe 23, a cooling water outlet pipe 24, a discharging pipe 25, an air inlet pipe 26, a discharging pipe valve 27 and a control switch valve 28.
The fermentation tank body 1 is cylindrical, the surface of the inner tank body is subjected to mirror polishing, process openings of inlet and outlet pipe orifices and welding positions of the inner tank body are in arc transition, the fermentation tank body is smooth and easy to clean, no dead angle exists, and reliability and stability of the production process are guaranteed.
The heating pipe 2 is rectangular in cross section and arranged on the outer wall of the fermentation tank body 1, the fermentation tank body 1 is heated in a rectangular structural form, the heating speed is high, heat transfer is uniform, the temperature in the fermentation tank body 1 is relatively constant, the control and the mastering are easy, the sudden situation caused by insufficient heat energy supply is avoided, the consumption and the utilization of heat energy are reasonably controlled, and the operation cost is effectively controlled.
The steel cylinder sleeve 3 is arranged on the outer side of the heating pipe 2 and is a closed container, circulating cooling water is filled in the steel cylinder sleeve, the cooling water enters the steel cylinder sleeve through a cooling water inlet pipe 23 and enters the steel cylinder sleeve through a water valve 12, the water valve 12 controls the flow of the inlet water, and the circulating water is heated and is conveyed out through a cooling water outlet pipe 24 during working.
The external heat-insulating layer 4 is made of superfine glass wool, and the arrangement of the external heat-insulating layer 4 ensures that the heat-insulating effect of the invention is better, and prevents the adverse effect of the change of the environmental temperature on the fermentation process.
The motor 5 is connected with the stirring shaft 8, the stirring shaft 8 is arranged in the fermentation tank body 1, the defoamer 7 is arranged at the upper end of the stirring shaft 8, and the plurality of stirrers 9 are arranged on the stirring shaft 8. The invention realizes the uniform mixing of the strains, improves the fermentation rate, improves the influence of temperature and pressure on the fermentation process in the fermentation process, and ensures the stable operation of the fermentation process.
The sterile shaft seal 6 is arranged in a gap at the joint part of the top of the fermentation tank body 1 and the stirring shaft 8. The sterile shaft seal 6 can effectively control the breeding condition of microorganisms and bacteria in the fermentation tank body 1, and is beneficial to improving the fermentation effect.
The air injection device 10 is arranged at the inner bottom of the fermentation tank body 1, a plurality of spray holes are formed in the air injection device 10 and communicated with an air filter 11 outside the fermentation tank body 1 through a pipeline, and the inlet end of the air filter 11 is communicated with an air inlet pipe 26.
The sight glass 13 locate 1 upper ends of fermentation tank, be convenient for observe the inside fermentation condition.
The digital display thermometer 14 is arranged at the upper end of the exterior of the fermentation tank body 1 and is connected with a temperature sensor 22 in the fermentation tank body 1 to indicate the working temperature in the fermentation tank body 1 at any time. The temperature sensor 22 can be composed of one or more than one, and is embedded on the inner side wall of the fermentation tank body 1.
The feed inlet 15 is arranged at the upper end of the fermentation tank body 1, and fermentation materials enter the fermentation tank body 1 from the feed inlet 15.
The pressure gauge 16 is arranged at the upper end of the fermentation tank body 1, the pressure gauge 16 observes the internal pressure, the automatic control valve 19 automatically controls the internal pressure, when the internal pressure of the fermentation tank body 1 is increased and exceeds the specified working pressure, the automatic control valve 19 is opened, the exhaust pipe 20 is used for exhausting gas, so that the internal pressure of the fermentation tank 1 is not more than the working pressure all the time, and meanwhile, the waste gas generated in the fermentation process is exhausted through the exhaust pipe 20, and the fermentation process is convenient to carry out.
PH meter 17 and dissolved oxygen detector 18 locate the fermentation tank body 1 upper end, and PH meter 17 and dissolved oxygen detector 18's lower extreme all peg graft in fermentation tank body 1, can detect the dissolved oxygen volume in the fermentation tank body 1 through dissolved oxygen detector 18, according to this value, steerable air advances pipe 26 and gets into the air volume to reach the oxygenation purpose.
The sampling tube 21 locate the fermentation tank body 1 upper end, and the lower extreme of sampling tube 21 is pegged graft in the fermentation tank body 1, sets up control switch valve 28 on the sampling tube 21, the pipeline of sampling tube 21 in the fermentation tank body 1 is the position that the lower extreme should exceed 1/2 fermentation tank body 1.
The discharging pipe valve 27 and the discharging pipe 25 are arranged at the outer lower end of the fermentation tank body 1, the upper end of the discharging pipe valve 27 is connected with the lower end of the fermentation tank body 1 through a pipeline, and the lower end of the discharging pipe valve is connected with the discharging pipe 25.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered as the technical scope of the present invention, and equivalents and modifications of the technical solutions and inventive concepts thereof should be covered by the scope of the present invention.

Claims (9)

1. Microbial fermentation tank for liquid feed additive production, which is characterized by comprising a fermentation tank body (1), a heating pipe (2), a steel cylinder sleeve (3), an outer heat-insulating layer (4), a motor (5), a sterile shaft seal (6), a foam cleaning device (7), a stirring shaft (8), a stirrer (9), an air injection device (10), an air filter (11), a water valve (12), a sight glass (13), a digital display thermometer (14), a feeding hole (15), a pressure gauge (16), a PH gauge (17), a dissolved oxygen detector (18), an automatic control valve (19), an exhaust pipe (20), a sampling pipe (21), a temperature sensor (22), a cooling water inlet pipe (23), a cooling water outlet pipe (24), a discharging pipe (25), an air inlet pipe (26), a discharging pipe valve (27) and a control switch valve (28).
2. The microbial fermentation tank for producing a liquid feed additive according to claim 1, wherein: the fermentation tank body (1) is cylindrical, the surface of the inner tank body is subjected to mirror polishing, process openings of the inlet and outlet pipe orifices and welding positions of the inner tank body are in arc transition, the fermentation tank body is smooth and easy to clean, no dead angle exists, and the reliability and stability of the production process are guaranteed.
3. The microbial fermentation tank for producing a liquid feed additive according to claim 1, wherein: the cross section of the heating pipe (2) is rectangular, the heating pipe is arranged on the outer wall of the fermentation tank body (1), the fermentation tank body (1) is heated in a rectangular structural form, the heating speed is accelerated, and the heat transfer is uniform.
4. The microbial fermentation tank for producing a liquid feed additive according to claim 1, wherein: the steel cylinder sleeve (3) is arranged on the outer side of the heating pipe (2) and is a closed container, circulating cooling water is filled in the closed container, the cooling water enters the pipe (23) through the cooling water and enters the closed container through a water valve (12), the water valve (12) controls the flow of the water, and the circulating water is heated and is conveyed out through a cooling water outlet pipe (24) during working.
5. The microbial fermentation tank for producing a liquid feed additive according to claim 1, wherein: the motor (5) is connected with the stirring shaft (8), the stirring shaft (8) is arranged inside the fermentation tank body (1), the defoamer (7) is arranged at the upper end of the stirring shaft (8), and the plurality of stirrers (9) are arranged on the stirring shaft (8).
6. The microbial fermentation tank for producing a liquid feed additive according to claim 1, wherein: the air injection device (10) is arranged at the inner bottom of the fermentation tank body (1), a plurality of spray holes are formed in the air injection device (10) and communicated with an air filter (11) outside the fermentation tank body (1) through a pipeline, and the inlet end of the air filter (11) is communicated with an air inlet pipe (26).
7. The microbial fermentation tank for producing a liquid feed additive according to claim 1, wherein: the digital display thermometer (14) is arranged at the upper end of the exterior of the fermentation tank body (1), is connected with the temperature sensor (22) in the fermentation tank body (1), and indicates the working temperature in the fermentation tank body (1) at any time.
8. The microbial fermentation tank for producing a liquid feed additive according to claim 1, wherein: the pressure gauge (16) is arranged at the upper end of the fermentation tank body (1), the pressure gauge (16) observes the internal pressure, and the automatic control valve (19) automatically controls the internal pressure.
9. The microbial fermentation tank for producing a liquid feed additive according to claim 1, wherein: the PH meter (17) and the dissolved oxygen detector (18) are arranged at the upper end of the fermentation tank body (1), and the lower ends of the PH meter (17) and the dissolved oxygen detector (18) are inserted into the fermentation tank body (1).
CN201810693352.8A 2018-06-29 2018-06-29 Microbial fermentation tank for producing liquid feed additive Pending CN110656043A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112300919A (en) * 2020-10-21 2021-02-02 南京延长反应技术研究院有限公司 Micro-interface fermentation system and fermentation method thereof
CN114164101A (en) * 2021-12-31 2022-03-11 山东润德生物科技有限公司 Microbial fermentation tank beneficial to promoting fermentation efficiency of glucosamine and application thereof
CN115254944A (en) * 2022-06-07 2022-11-01 福建永强岩土股份有限公司 Soil remediation method based on application of microorganisms and remediation device thereof
WO2023108311A1 (en) * 2021-12-13 2023-06-22 山西农业大学山西功能食品研究院 Fruit and vegetable enzyme fermentation equipment for maintaining biological activity

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112300919A (en) * 2020-10-21 2021-02-02 南京延长反应技术研究院有限公司 Micro-interface fermentation system and fermentation method thereof
WO2023108311A1 (en) * 2021-12-13 2023-06-22 山西农业大学山西功能食品研究院 Fruit and vegetable enzyme fermentation equipment for maintaining biological activity
CN114164101A (en) * 2021-12-31 2022-03-11 山东润德生物科技有限公司 Microbial fermentation tank beneficial to promoting fermentation efficiency of glucosamine and application thereof
CN115254944A (en) * 2022-06-07 2022-11-01 福建永强岩土股份有限公司 Soil remediation method based on application of microorganisms and remediation device thereof
CN115254944B (en) * 2022-06-07 2023-12-01 福建永强岩土股份有限公司 Soil restoration method and restoration device based on microorganism application

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