JP3665102B2 - Microbial culture system - Google Patents

Description

[0001]
[Industrial application fields]
The present invention relates to a system for culturing microorganisms serving as feed for other organisms, for example, phytoplankton serving as feed for shellfish and crustaceans.
[0002]
[Prior art]
When culturing shellfish and crustaceans in a breeding tank, phytoplankton (diatoms), which are the feed, are cultured in another culture apparatus and fed to the breeding tank.
Conventionally, culturing of phytoplankton as feed is difficult to adjust in terms of nutrition, air, and light heat, and thus relies on manual work by skilled workers.
In addition, there is an aquarium for breeding aquatic organisms that maintains a biological breeding environment known from JP-A-1-309630.
[0003]
[Problems to be solved by the invention]
For laborious feed culture, it is desirable to save labor by automatic control.
However, automatic control, on the other hand, has a problem that leads to higher costs.
Further, conventionally, there is a problem that culture failure occurs due to abnormalities such as contamination with bacteria, and stable production is difficult.
In addition, in automatic control, there is a risk that such an abnormal situation will not be noticed and further an increase in culture defects will be caused. The above-mentioned known water tank could not solve the culture failure due to contamination with bacteria.
[0004]
[Means for Solving the Problems]
The present invention uses the following means in order to solve the above problems. This is a system for switching the flow of the culture liquid CL in one step of the microorganism culture system by valve opening / closing control and automatic control of the metering pump, and adjusting and mixing microorganisms and nutrient solution to make the culture liquid CL 7 is introduced into the culture tank 10 through the supply / discharge pipe 9 and the culture liquid CL adjusted and mixed in the culture liquid adjustment tank 7 is supplied to the culture tank 10 to supply light heat and air. After culturing, the culture liquid CL in the culture tank 10 after completion of the culture is taken out using the supply / discharge pipe 9 also for discharge, and is fed into the breeding tank 16 for other organisms through the feed tank 13. The planting pipe 17 is connected to the supply / discharge pipe 9 from the culture tank 10 to the feed tank 13, and the other end of the planting pipe 17 is piped to the culture solution adjusting tank 7. mixed culture liquid CL part of the culture solution to the culture medium adjusting tank 7 Out adopted as use, air is supplied into the culture tank in 10 culture in also configured to introduce the feed discharge tube 9 from the air tube 12, the air introduction part from the air pipe 12 to the supply and discharge pipe 9 In this case, it is formed directly above the opening / closing control valve V2 provided in the supply / discharge pipe 9 connected to the bottom of the culture tank 10.
[0005]
[Action]
In the microbial culture system, the culture fluid supply pipe to the culture tank is the same as the culture liquid discharge pipe in the culture tank, and the flow of the liquid is changed by valve opening / closing control to supply and discharge in the same pipe. It is possible.
[0006]
Thereby, piping cost is reduced and the high cost accompanying automatic control is offset.
Furthermore, by automatically controlling the microbial culture system, the conventional manual work is omitted, and labor is saved.
[0007]
【Example】
Embodiments of the present invention will be described with reference to the accompanying drawings.
FIG. 1 is a system block diagram of a microorganism culture system of the present invention,
Figure 2 shows the system piping diagram.
FIG. 3 is a side view of the culture tank.
[0008]
The overall flow of the microorganism culture system of the present invention will be described with reference to FIGS.
In this embodiment, phytoplankton (diatom M), for example, Keatoceros gracilis, Pavlova, etc., is cultivated as feed for shellfish such as shallow sea breams raised in seawater in a breeding tank.
First, the process of adjusting and mixing the culture solution in the culture solution adjusting tank 7 shown in FIG. 2 (<5> culture solution adjustment shown in FIG. 1) will be described.
[0009]
The seawater SW, which is the main liquid of the culture solution, is filtered by the filter 2 through the seawater supply pipe 1 in FIG. 1 (<1> seawater microfiltration) and sterilized by ultraviolet rays 3 (<2> seawater UV (ultraviolet rays) sterilization). ), And fed into the culture medium adjustment tank 7.
In addition, a stock solution of nutrient VL necessary for culture is placed in the injection bottle 5 and adjusted and stored in the cold storage chamber 4 (for example, 5 ° C.) (<3> nutrient adjustment shown in FIG. 1). A necessary amount of nutrient VL is supplied into the culture solution adjusting tank 7 through the nutrient supply pipe 6.
The diatom M to be cultured is partly extracted in the subsequent <7> culture process shown in FIG. 1 or <8> harvest process (<4> planting shown in FIG. 1). This is supplied into the culture solution adjusting tank 7. Thus, the sterilized seawater SW, the nutrient salt VL, and the diatom M are mixed in the culture solution adjustment tank 7, and the culture solution is adjusted while adjusting the pH by the pH controller 8.
[0010]
The culture liquid CL adjusted in the culture liquid adjustment tank 7 is supplied and discharged from the supply / discharge pipe 9 to the bottom surface 10d of the culture tanks 10 and 10 in the cold storage chamber (for example, 10 ° C. to 20 ° C.). 9 is supplied through the tube end opening 9c (shown in FIG. 3) (<6> culture tank adjustment shown in FIG. 1).
The culture tanks 10, 10... Are supplied with light and heat necessary for the photosynthesis of the diatom M from the fluorescent lamp 11, and the air pipe 12 is introduced into the supply / discharge pipe 9 as shown in FIG. 10 · 10... Stirred and mixed in the culture solution, and the supply of air A for diatom M culture is floated from the tube end opening 9c of the supply / discharge tube 9 opened to the bottom surface 10d. (<7> culture shown in FIG. 1).
[0011]
Thus, when the diatom M is cultured (for example, for 6 days) and the culture liquid CL in the culture tank 10 can be supplied as feed, the culture liquid CL is supplied as feed F through the supply / discharge pipe 9. Then, it is supplied to the feed tank 13 and stored in a certain amount (<8> harvest shown in FIG. 1), and is supplied to the breeding tanks 16, 16... 1 <9> feeding as shown).
[0012]
As described above, during the <8> harvesting (<7> culture shown in FIG. 1) process shown in FIG. 1, a part of the diatom M supplied into the culture medium adjustment tank 7 via the supply / discharge pipe 9 is obtained. In FIG. 1, the planting pipe 17 is piped from the feed tank 13 to the culture solution adjusting tank 7.
The culture medium adjustment tank 7 and the feed tank 13 are each supplied with air A by air pipes 18 and 19, and the culture medium adjustment tank 7 is supplied to the supply / discharge pipe 9, The feed tank 13 is supplied with air introduced into the drain pipe 14.
[0013]
The cleaning liquid WL is supplied to the culture liquid adjustment tank 7, the culture tank 10, the feed tank 13, and the supply / discharge pipe 9 from the cleaning stock solution tank 20 and the water receiving tank 21, thereby enabling internal cleaning. (<10> water tank cleaning shown in FIG. 1).
The cleaning stock solution tank 20 is supplied with the sodium hypochlorite solution of the cleaning stock solution, and the tap water W is supplied into the water receiving tank 21 to store the tap water W. 22 is supplied to each part as a cleaning liquid WL. For the culture liquid adjustment tank 7, the culture tank 10, and the feed tank 13, a nozzle 23 is attached to the end of the cleaning liquid pipe 22. The cleaning liquid WL is jetted from above. The inside of the supply / discharge pipe 9 is cleaned by passing the cleaning liquid WL.
[0014]
In the microorganism culture system in which diatom M is cultured and fed as described above, and in which water tanks and pipes are washed, each pipe is provided with various valves such as an electromagnetic valve and a pressure regulating valve. The automatic opening / closing control based on various sensors and controllers makes it possible to automatically adjust the supply and discharge amounts of seawater SW, nutrient VL, culture solution CL, feed F, air A, and cleaning solution WL.
[0015]
In addition, a metering pump P (diaphragm pump or the like) is interposed at the seawater supply pipe 1, the nutrient salt supply pipe 6, the feeding pipe 15, the planting pipe 17, and the washing stock solution tank 20 outlet and the water receiving tank 21 outlet, A predetermined amount of seawater SW, nutrient salt VL, feed F (including those for planting), cleaning stock solution, and tap water W can be passed.
In addition, as shown in FIG. 1, the adjustment work of a nutrient salt is a manual work, and the planting can be automatically controlled by a metering pump P as shown in FIG.
[0016]
In the microorganism culture system having the above configuration and flow, the configuration of each unit will be described.
First, the supply / discharge pipe 9 will be described.
As shown in FIG. 2, the culture solution CL and the feed F are supplied and discharged through the same supply / discharge pipe 9 between the culture solution adjustment tank 7, the culture tanks 10, and the feed tank 13. .
Further, as shown in FIG. 2, the arrangement height is such that the culture solution adjustment tank 7 is at the top, the culture tank 10 is at the upper and lower intermediate positions, and the feed tank 13 is at the bottom.
[0017]
Therefore, if the valve V1, valves V2, V2,... Are opened and the valve V3 is closed in the supply / discharge pipe 9, the culture medium CL can be supplied from the culture medium adjustment tank 7 to each culture tank 10. At this time, in the branch pipe 9a to each culture tank 10, the culture solution CL flows in the direction of the arrow in FIG.
Then, when the valve V1 is closed and the valves V2, V2,... And the valve V3 are opened, the feed F is supplied from each culture tank 10 to the feed tank 13. At this time, the feed F flows in the direction of the arrow in the branch pipe 9a.
The valve V4 is normally closed, and the culture liquid CL or the feed F in the pipe can be drained by opening this valve and closing the other valves. As described above, supply and discharge can be performed in the same pipe by opening and closing the valve, so that the piping cost can be saved and the operation can be facilitated.
[0018]
Next, the structure of the culture tank 10 is demonstrated from FIG.
First, the air supply configuration into the culture liquid CL will be described. As described above, the supply of air A into the culture liquid CL in each culture tank 10 is introduced from the air pipe 12 into the supply / discharge pipe 9. The introduction site from the air pipe 12 is directly above the valves V2, V2,... Provided in the distribution pipes 9b, 9b, etc. connected to the bottom of each culture tank 10, that is, the culture tank. 10 is located directly below the bottom.
[0019]
The reason for introducing the air A into this part will be described.
Conventionally, an air tube (glass tube or airstone attached) is introduced into the culture tank 10 from above, and the end of the tube is immersed in the culture solution CL. More directly, air is introduced into the culture solution CL in the culture tank 10.
However, in this case, when the culture tank 10 is cleaned, the back side of the air tube immersed in the culture solution CL is difficult to be cleaned, which causes insufficient cleaning. Insufficient washing causes the generation of germs and adversely affects the culture.
If air is introduced into the supply / discharge pipe 9, the air pipe is not immersed in the culture solution CL in the culture tank 10, thus eliminating the situation of insufficient cleaning of the back side of the air pipe.
[0020]
And as mentioned above, if it introduce | transduces into the upper part of valve | bulb V2 of the distribution pipe 9b below the bottom part of the culture tank 10, if air is not introduce | transduced into this site | part, a sag will arise in this site | part during culture | cultivation. From there, harmful substances such as various bacteria and hydrogen sulfide are generated, causing an oxygen deficient state in the culture solution CL and adversely affecting the culture.
Therefore, by introducing air into this part, the culture liquid CL in this part is agitated and no stagnation occurs. Accordingly, since the air is also introduced into the distribution pipe 9b and then moved upward, there is no place where stagnation occurs if the air is introduced directly above the lowermost valve V2. This configuration is also applied to the culture medium adjustment tank 7 and the feed tank 13.
[0021]
Next, the configuration of the lid 10a of the culture tank 10 will be described.
The lid 10a is easily removed, and is made transparent so that the state of the internal culture solution CL can be easily observed from the outside. And the pipe | tube end of the washing hose 22a connected from the washing | cleaning liquid pipe | tube 22 is fixed, and the nozzle 23 is suspended by the lower part of the lid | cover 10a.
By using a flexible hose, the lid 10a can be removed freely. The nozzle 23 is rotatable, and by rotating and spraying the washing liquid, the inside of the culture tank 10 can be cleaned well, and the inside of the culture tank 10 is kept in a state of being covered. In addition, it is not necessary to provide a cleaning nozzle in the culture tank 10 for each cleaning, and if the cleaning liquid WL is supplied into the cleaning liquid tube 22, the cleaning liquid is jetted naturally from the nozzle. Then, the tank is cleaned.
This configuration is also applied to the culture solution adjusting tank 7 and the feed tank 13. Further, the lid 10a is provided with an exhaust port 10b, and a gap 10c can be formed between the culture tank 10 and the lid 10a. After the air introduced from 9b is mixed in the culture liquid CL in the culture tank 10, it is exhausted from the upper surface of the culture liquid CL by respiration or photosynthesis of the diatom M. This exhaust is exhausted from the exhaust port 10b. Thus, respiration and photosynthesis can be activated.
[0022]
In addition, since the culture liquid CL (feed F) in the culture tank 10 is supplied and discharged through the distribution pipe 9b connected to the bottom, the bottom surface 10d of the culture tank 10 is tapered so that the flow of the liquid is smoothed and discharged. Later, the liquid is less likely to remain in the culture tank 10. The fluorescent lamp 11 is irradiated from the side surface so that light can reach the inside of the culture tank 10 widely, and the fluorescent lamp 11 is controlled by a timer to activate the culture.
[0023]
Next, although the electromagnetic valve V5 is interposed in the air pipe 12, the valve is opened when not energized during normal times and is closed when energized. That is, normally, air is sent to the culture tank 10, the culture solution adjusting tank 7, and the feed tank 13, and the air is supplied to the culture solution CL or the feed F to relieve stagnation by breathing and stirring. As a configuration in which air supply is stopped only when washing is limited, cost reduction can be realized. If the electromagnetic valve V5 is deenergized due to a power failure or the like, air supply is performed in the open state. Therefore, the safety of the culture tank CL and the feed F in the culture tank 10 and the like can be maintained.
[0024]
The air pipe 12 is usually supplied with O2 for respiration, but it is also possible to supply CO2 for photosynthesis or for pH adjustment. In addition, the cleaning liquid WL can be supplied to the air pipe 12 and the air pipes 18 and 19 (not shown in FIG. 2), and the inside of the pipe can be cleaned. In addition, an air filter is provided for air purification.
[0025]
【The invention's effect】
Since the present invention is configured as described above, the following effects can be obtained.
Since the culture medium supply pipe for the culture tank and the culture liquid discharge pipe for the culture tank are the same pipe, piping costs are reduced, offsetting the high costs due to automatic control, and maintenance work related to the pipes. Reduced.
In addition, the air supplied into the culture solution CL in the culture tank 10 is also configured to be introduced into the supply / discharge pipe 9 from the air pipe 12, and the air introduction site from the air pipe 12 to the supply / discharge pipe 9 includes The air pipe is not immersed in the culture solution CL in the culture tank 10 because it is directly above the opening / closing control valve V2 provided in the supply / discharge pipe 9 connected to the bottom of the culture tank 10. The situation of lack of cleaning on the back side of can be solved.
[0026]
Moreover, since it introduce | transduced into the valve | bulb V2 upper part of the supply / discharge pipe | tube 9 in the downward direction of the bottom part of the culture tank 10, the culture solution CL in this site | part will be stirred and it will not produce stagnation.
Therefore, since the air is introduced into the distribution pipe 9b and then moves upward, the air introduction location is located directly above the lowermost opening / closing control valve V2 so that no stagnation occurs. .
[0027]
In addition, a planting pipe 17 is connected to the supply / discharge pipe 9 from the culture tank 10 to the feed tank 13, and the other end of the planting pipe 17 is connected to the culture solution adjusting tank 7. Since a part of the sample was taken out for mixing the culture solution CL into the culture solution adjustment tank 7, the planting operation of the culture solution CL in the culture solution adjustment tank 7 was automatically performed. is there.
Moreover, since the process of a series of microorganism culture systems is automatically controlled, labor saving can be realized. In addition, culturing of phytoplankton as feed is difficult to adjust in terms of nutrition, air, and light heat, so it is possible to improve the point of relying on manual work by skilled workers.
[Brief description of the drawings]
FIG. 1 is a system block diagram of a microorganism culture system of the present invention.
FIG. 2 is also a system piping diagram.
FIG. 3 is a side view of a culture tank.
[Explanation of symbols]
M Diatom SW Seawater VL Nutrient CL Culture fluid F Feed A Air W Tap water WL Cleaning fluid V1-V5 Valve (Electromagnetic valve)
P Metering pump 7 Culture medium adjustment tank 9 Supply / discharge pipe 9a Branch pipe 9b Distribution pipe 10 Culture tank 10a Lid 10b Exhaust port 11 Fluorescent lamp 12 Air pipe 13 Feed tank 16 Breeding tank 17 Planting pipe 20 Washing stock tank 21 Receiving tank 22 Cleaning liquid tube 22a Cleaning hose 23 Cleaning nozzle

Claims (1)

A system for switching the flow of the culture liquid CL in one step of the microorganism culture system by valve opening / closing control and metering pump automatic control,
Cultures adjustment tank 7 mixed adjusted microorganism and nutrient solution and culture solution CL is provided, introduction of culture CL adjusted contaminated in the culture medium adjusting tank 7, via the supply and discharge pipe 9 into the culture tank 10 Then, in the culture tank 10, microorganisms are cultured by supplying light heat and air, and the culture liquid CL in the culture tank 10 after the completion of the culture is taken out using the supply / discharge pipe 9 as a discharge, Through the feed tank 13, it is configured to be given as food in the breeding tank 16 of other organisms,
A planting pipe 17 is connected to the supply / discharge pipe 9 from the culture tank 10 to the feed tank 13, and the other end of the planting pipe 17 is connected to the culture solution adjusting tank 7. A portion for mixing the culture fluid CL into the culture fluid conditioning tank 7,
The air supplied into the culture medium in the culture tank 10 is also configured to be introduced into the supply / discharge pipe 9 from the air pipe 12, and the air introduction site from the air pipe 12 to the supply / discharge pipe 9 is provided in the culture tank 10. A microorganism culture system characterized in that it is provided directly above an opening / closing control valve V2 interposed in a supply / discharge pipe 9 connected to the bottom.

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