JP4245448B2 - Microbial culture system - Google Patents

Description

  The present invention relates to a system for culturing microorganisms serving as feed for other organisms, for example, phytoplankton serving as feed for shellfish and crustaceans.

  When culturing shellfish and crustaceans in a breeding tank, phytoplankton (diatoms), which are the feed, are cultured in another culture apparatus and fed to the breeding tank. Conventionally, culturing of phytoplankton as feed is difficult to adjust in terms of nutrition, air, and light heat, and thus relies on manual work by skilled workers. In addition, there is an aquarium for breeding aquatic organisms that maintains a biological breeding environment known from JP-A-1-309630.

JP-A-1-309630

  In the configuration in which air is supplied to the culture tank of the microorganism culture system, the inside of the exhaust pipe from the culture tank to the on-off valve is agitated so that no stagnation occurs and there is no risk of generation of various bacteria. Furthermore, the culture solution in the culture tank is also stirred so as to contribute to respiration and promotion of photosynthesis. And since the air tube immersed in a culture solution is deleted like the past, the situation of a washing defect is eliminated. As a result, culture defects are reduced and stable production of feed is possible. In addition, the air tube open / close valve is closed when energized, so that air is always supplied to the culture tank during normal operation when it is not energized, and has a stirring action. Since it is ensured, it is safe, and since the normal time is not energized, the cost is reduced.

  The present invention uses the following means in order to solve the above problems.

The culture solution prepared by mixing and mixing the nutrient solution in the culture solution adjusting tank (7) and the microorganism are put into the culture tank (10), and the microorganism is cultured by supplying light heat and air, and the culture tank (10). While the culture medium in the inside is supplied into the feed tank (13) and stored in a certain amount, it is supplied to the breeding tank (16) through the feeding pipe (15), and is given as food to the breeding tank of other organisms. In the microorganism culture system for extracting a part of the microorganisms for mixing into the culture solution, the culture solution adjustment tank (7), the culture tanks (10), and the feed tank (13) are cultivated between each other. The supply / discharge of the liquid (CL) and the feed (F) is configured to be performed in the same supply / discharge pipe (9), and the supply / discharge pipe (9) suspended from the bottom of the culture tank (10) An opening / closing valve (V2) is provided, which is directly above the opening / closing valve (V2), that is, directly below the bottom of the culture tank (10). The supply of culture medium to the culture vessel (10), the feed discharge tube (9) unit for performing the discharge of the culture solution from the culture tank (10), an air tube (12) communicates, the air pipe (12) The air for culturing microorganisms is supplied via the air pipe, the electromagnetic valve (V5) is provided in the air pipe (12), and the electromagnetic valve (V5) is closed when energized.

  In such a microbial culture system, in the culture tank, this part is stirred by supplying air to the upper part of the open / close valve in the discharge pipe, which is prone to stagnation during culture. Stir the entire culture to avoid itchiness that may cause miscellaneous bacteria.

  In addition, the air pipe is closed when energized, that is, it is configured to open and feed air to the culture tank during normal non-energization. The effect is brought about, and the culture failure is eliminated.

Since the present invention is configured as described above, the following effects can be obtained.
Since the microorganism culture system is configured to supply air to the culture tank, the inside of the exhaust pipe from the culture tank to the on-off valve is agitated, so that no stagnation occurs and there is no possibility of generation of germs. Furthermore, the culture solution in the culture tank is also agitated, contributing to the promotion of respiration and photosynthesis. And since the air tube immersed in a culture solution is deleted like the past, the situation of a washing defect is eliminated. As a result, culture defects are reduced and stable production of feed is possible.

  In addition, since the air tube open / close valve is closed when energized, air is always supplied to the culture tank during normal operation when de-energized, and this has a stirring action. Since the supply is ensured, there is safety, and the cost is low because the normal time is when no power is supplied.

  Embodiments of the present invention will be described with reference to the accompanying drawings. FIG. 1 is a system block diagram of a microorganism culture system of the present invention, FIG. 2 is a system piping diagram, and FIG. 3 is a side view of a culture tank.

  The overall flow of the microorganism culture system of the present invention will be described with reference to FIGS. In this embodiment, phytoplankton (diatom M), for example, Keatoceros gracilis, Pavlova, etc., is cultivated as feed for shellfish such as shallow sea breams raised in seawater in a breeding tank. First, the process of adjusting and mixing the culture solution in the culture solution adjustment tank 7 shown in FIG. 2 (<5> culture solution adjustment shown in FIG. 1) will be described. The solution is filtered through the supply pipe 1 with the filter 2 (<1> seawater microfiltration), sterilized with ultraviolet rays 3 (<2> seawater UV (ultraviolet rays) sterilized), and supplied into the culture solution adjustment tank 7. In addition, a stock solution of nutrient VL necessary for culture is placed in the injection bottle 5, adjusted and stored in the cold storage chamber 4 (for example, 5 ° C.) (<3> nutrient adjustment) The salt VL is supplied into the culture solution adjusting tank 7 through the nutrient salt supply pipe 6.

  The diatom M to be cultured is partly extracted in the subsequent <7> culture process or <8> harvest process (<4> planting), and this is put in the culture solution adjusting tank 7. Supply. Thus, the sterilized seawater SW, the nutrient salt VL, and the diatom M are mixed in the culture solution adjustment tank 7, and the culture solution is adjusted while adjusting the pH by the pH controller 8.

  The culture liquid CL adjusted in the culture liquid adjustment tank 7 is supplied and discharged from the supply tank 9 into the culture tanks 10, 10... In the cold storage chamber (for example, 10 ° C. to 20 ° C.). 9 is supplied through the tube end opening 9c (<6> culture tank adjustment). The culture tanks 10, 10... Are supplied with light and heat necessary for the photosynthesis of the diatom M from the fluorescent lamp 11, and the air pipe 12 is introduced into the supply / discharge pipe 9. .. Stirring and mixing in the culture medium, and supplying air A for diatom M culture is caused to float from the tube end opening 9c of the supply / discharge tube 9 opened to the bottom surface 10d (<7> culture).

  Thus, when the diatom M is cultured (for example, for 6 days) and the culture liquid CL in the culture tank 10 can be supplied as feed, the culture liquid CL is supplied as feed F through the supply / discharge pipe 9. Then, it is supplied into the feed tank 13 and stored in a certain amount (<8> harvest), and is supplied to the feeding tanks 16, 16... For raising shellfish through the feeding pipe 15 (<9> feeding). . As described above, during the <8> harvesting (<7> culturing) process, a part of the diatom M is supplied into the culture solution adjusting tank 7 through the supply / discharge pipe 9, and <4> transplanted. However, the planting pipe 17 is piped from the feed tank 16 to the culture solution adjustment tank 7. The culture medium adjustment tank 7 and the feed tank 13 are each supplied with air A by air pipes 18 and 19, and the culture medium adjustment tank 7 is supplied to the supply / discharge pipe 9, The feed tank 13 is supplied with air introduced into the drain pipe 14.

  The cleaning liquid WL is supplied to the culture liquid adjustment tank 7, the culture tank 10, the feed tank 13, and the supply / discharge pipe 9 from the cleaning stock solution tank 20 and the water receiving tank 21, thereby enabling internal cleaning. (<10> aquarium cleaning). The cleaning stock solution tank 20 is supplied with a sodium hypochlorite solution of the cleaning stock solution, and the water receiving tank 21 is supplied with tap water W to store the tap water W. These are stored in the cleaning solution pipe 22. It is introduced into the inside and supplied to each part as a cleaning liquid WL. For the culture liquid adjustment tank 7, the culture tank 10, and the feed tank 13, a nozzle is attached to the end of the pipe, and the cleaning liquid WL is supplied from above. It is the composition which is injected. The inside of the supply / discharge pipe 9 is cleaned by passing the cleaning liquid WL.

  In the microorganism culture system in which diatoms are cultured and fed as described above, and in which a water tank and pipes are washed, each pipe is provided with various valves such as an electromagnetic valve and a pressure regulating valve. Automatic opening / closing control based on various sensors and controllers makes it possible to automatically adjust the supply and discharge amounts of seawater SW, nutrient VL, culture solution CL, feed F, air A, and cleaning solution WL. In addition, a metering pump P (diaphragm pump or the like) is interposed at the seawater supply pipe 1, the nutrient salt supply pipe 6, the feeding pipe 15, the planting pipe 17, the outlet of the cleaning stock solution tank 20 and the outlet of the water receiving tank 21, A predetermined amount of seawater SW, nutrient salt VL, feed F (including those for planting), cleaning stock solution, and tap water W can be passed. In addition, as shown in FIG. 1, the adjustment work of nutrient salt is a manual work, and planting can be automatically controlled by a metering pump P as shown in FIG. It is supposed to be.

  In the microorganism culture system having the above configuration and flow, the configuration of each unit will be described. First, the supply / discharge pipe 9 will be described. As shown in FIG. 2, the culture solution CL and the feed F are supplied and discharged through the same supply / discharge pipe 9 between the culture solution adjustment tank 7, the culture tanks 10, and the feed tank 13. . Further, as shown in FIG. 2, the arrangement height is such that the culture solution adjustment tank 7 is at the top, the culture tank 10 is at the upper and lower intermediate positions, and the feed tank 13 is at the bottom. Therefore, if the valve V1, valves V2, V2,... Are opened and the valve V3 is closed in the supply / discharge pipe 9, the culture medium CL can be supplied from the culture medium adjustment tank 7 to each culture tank 10.

  At this time, in the branch pipe 9a to each culture tank 10, the culture solution CL flows in the direction of the arrow in FIG. Then, when the valve V1 is closed and the valves V2, V2,... And the valve V3 are opened, the feed F is supplied from each culture tank 10 to the feed tank 13. At this time, the feed F flows in the direction of the arrow in the branch pipe 9a. The valve V4 is normally closed, and the culture liquid CL or the feed F in the pipe can be drained by opening this valve and closing the other valves. As described above, supply and discharge can be performed in the same pipe by opening and closing the valve, so that the piping cost can be saved and the operation can be facilitated.

  Next, the structure of the culture tank 10 is demonstrated from FIG. First, the air supply configuration into the culture liquid CL will be described. As described above, the air supply into the culture liquid CL in each culture tank 10 is introduced from the air pipe 12 into the supply / discharge pipe 9. The introduction portion from the air pipe 12 is directly above the valves V2, V2,... Interposed in the distribution pipes 9b, 9b, etc. connected to the bottom of each culture tank 10, that is, the culture tank 10 It is directly below the bottom.

  The reason why air is introduced into this part will be described. Conventionally, an air tube (glass tube or airstone attached) is introduced into the culture tank 10 from above, and the end of the tube is immersed in the culture solution CL. More directly, air is introduced into the culture solution CL in the culture tank 10. However, in this case, at the time of cleaning the culture tank 10, the back side (portion indicated by the arrow) of the air tube immersed in the culture solution CL is difficult to be cleaned, causing insufficient cleaning. Insufficient washing causes the generation of germs and adversely affects the culture. If air is introduced into the supply / discharge pipe 9, the air pipe is not immersed in the culture solution CL in the culture tank 10, thereby eliminating the situation of insufficient washing.

  And as mentioned above, if it introduce | transduces into the upper part of valve | bulb V2 of the distribution pipe 9b below the bottom part of the culture tank 10, if air is not introduce | transduced into this site | part, a sag will arise in this site | part during culture | cultivation. From there, harmful substances such as various bacteria and hydrogen sulfide are generated, causing an oxygen deficient state in the culture solution CL and adversely affecting the culture. Therefore, by introducing air into this part, the culture liquid CL in this part is agitated and no stagnation occurs. Accordingly, since the air is also introduced into the distribution pipe 9b and then moved upward, there is no place where stagnation occurs if the air is introduced directly above the lowermost valve V2. This configuration is also applied to the culture medium adjustment tank 7 and the feed tank 13.

  Next, the configuration of the lid 10a of the culture tank 10 will be described. The lid 10a is easily removed, and is made transparent so that the state of the internal culture solution CL can be easily observed from the outside. And the pipe | tube end of the washing hose 22a connected from the washing | cleaning liquid pipe | tube 22 is fixed, and the nozzle 23 is suspended by the lower part of the lid | cover 10a. By using a flexible hose, the lid 10a can be removed freely.

  The nozzle 23 is rotatable, and by rotating and spraying the washing liquid, the inside of the culture tank 10 can be cleaned well, and the inside of the culture tank 10 is kept in a state of being covered. In addition, it is not necessary to provide a cleaning nozzle in the culture tank 10 for each cleaning, and if the cleaning liquid WL is supplied into the cleaning liquid tube 22, the cleaning liquid is jetted naturally from the nozzle. Then, the tank is cleaned. This configuration is also applied to the culture solution adjusting tank 7 and the feed tank 13. Further, the lid 10a is provided with an exhaust port 10b, and a gap 10c can be formed between the culture tank 10 and the lid 10a. After the air introduced from 9b is mixed in the culture liquid CL in the culture tank 10, it is exhausted from the upper surface of the culture liquid CL by respiration or photosynthesis of the diatom M. This exhaust is exhausted from the exhaust port 10b. Thus, respiration and photosynthesis can be activated.

  In addition, since the culture liquid CL (feed F) in the culture tank 10 is supplied and discharged through the distribution pipe 9b connected to the bottom, the bottom surface 10d of the culture tank 10 is tapered so that the flow of the liquid is smoothed and discharged. Later, the liquid is less likely to remain in the culture tank 10. The fluorescent lamp 11 is irradiated from the side surface so that light can reach the inside of the culture tank 10 widely, and the fluorescent lamp 11 is controlled by a timer to activate the culture.

  Next, although the electromagnetic valve V5 is interposed in the air pipe 12, the valve is opened when not energized during normal times and is closed when energized. That is, normally, air is sent to the culture tank 10, the culture solution adjusting tank 7, and the feed tank 13, and the air is supplied to the culture solution CL or the feed F to relieve stagnation by breathing and stirring. As a configuration in which air supply is stopped only when washing is limited, cost reduction can be realized. If the electromagnetic valve V5 is deenergized due to a power failure or the like, air supply is performed in the open state. Therefore, the safety of the culture tank CL and the feed F in the culture tank 10 and the like can be maintained.

In addition, breathing O ₂ is usually supplied into the air tube 11, but CO ₂ can also be supplied for photosynthesis and pH adjustment. In addition, the cleaning liquid WL can be supplied to the air pipe 11 and the air pipes 18 and 19 (not shown in FIG. 2), and the inside of the pipe can be cleaned. In addition, an air filter is provided for air purification.

It is a system block diagram of the microorganism culture system of this invention. It is also a system piping diagram. It is a side view of a culture tank.

Explanation of symbols

M Diatom SW Seawater VL Nutrient salt CL Culture fluid F Feed A Air W Tap water WL Cleaning fluid V1-V5 Valve (Electromagnetic valve)
P Metering pump 7 Culture medium adjustment tank 9 Supply / discharge pipe 9a Branch pipe 9b Distribution pipe 10 Culture tank 10a Lid 10b Exhaust port 11 Fluorescent lamp 12 Air pipe 13 Feed tank 16 Breeding tank 17 Planting pipe 20 Washing stock tank 21 Receiving tank 22 Cleaning liquid tube 22a Cleaning hose 23 Cleaning nozzle

Claims (1)

The culture solution prepared by mixing and mixing the nutrient solution in the culture solution adjusting tank (7) and the microorganism are put into the culture tank (10), and the microorganism is cultured by supplying light heat and air, and the culture tank (10). While the culture medium in the inside is supplied into the feed tank (13) and stored in a certain amount, it is supplied to the breeding tank (16) through the feeding pipe (15), and is given as food to the breeding tank of other organisms. In the microorganism culture system for extracting a part of the microorganisms for mixing into the culture solution, the culture solution adjustment tank (7), the culture tanks (10), and the feed tank (13) are cultivated between each other. The supply / discharge of the liquid (CL) and the feed (F) is configured to be performed in the same supply / discharge pipe (9), and the supply / discharge pipe (9) suspended from the bottom of the culture tank (10) An opening / closing valve (V2) is provided, which is directly above the opening / closing valve (V2), that is, directly below the bottom of the culture tank (10). The supply of culture medium to the culture vessel (10), the feed discharge tube (9) unit for performing the discharge of the culture solution from the culture tank (10), an air tube (12) communicates, the air pipe (12) The air for culturing microorganisms is supplied via the air pipe, the electromagnetic valve (V5) is interposed in the air pipe (12), and the electromagnetic valve (V5) is closed when energized. Microbial culture system.