JP2004000299A - Microbial cultivation system - Google Patents

Microbial cultivation system Download PDF

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JP2004000299A
JP2004000299A JP2003334024A JP2003334024A JP2004000299A JP 2004000299 A JP2004000299 A JP 2004000299A JP 2003334024 A JP2003334024 A JP 2003334024A JP 2003334024 A JP2003334024 A JP 2003334024A JP 2004000299 A JP2004000299 A JP 2004000299A
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culture
tank
supplied
air
solution
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JP4245448B2 (en
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Takitaro Ozaka
尾坂　滝太郎
Akira Shitsuetsu
室越　章
Atsutoshi Masuda
増田　篤稔
Hirohisa Hatada
畠田　裕久
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Yanmar Co Ltd
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Yanmar Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate

Abstract

<P>PROBLEM TO BE SOLVED: To provide a microbial cultivation system wherein risk of contamination is removed by preventing stagnation by supplying air to and stirring inside an exhaust pipe running between a culture tank and a switch valve and respiration and photosynthesis are promoted by stirring a culture solution inside the culture tank. <P>SOLUTION: In the microbial cultivation system, the culture liquid prepared by mixing adjusted amounts of microbes and a nutrient solution is supplied to the culture tank, light, heat and air are supplied to the tank for cultivating the microbes, and a portion of the culture liquid inside the tank is recovered to be blended into the culture solution while the remainder is supplied to a growth tank of other organisms as their feed. In the system, air for cultivating the microbes is supplied to a part just above the switching valve V2 inside the exhaust pipe 9b which vertically extends downward from the bottom of the culture tank 10. <P>COPYRIGHT: (C)2004,JPO

Description

　本発明は、他の生物の飼料となる微生物、例えば、貝類や甲殻類の飼料となる植物性プランクトンを培養するためのシステムに関する。 The present invention relates to a system for culturing microorganisms serving as feed for other organisms, for example, phytoplankton serving as feed for shellfish and crustaceans.

　貝類や甲殻類を飼育槽にて養殖するに当たっては、その飼料となる植物プランクトン（珪藻類）を他の培養装置にて培養し、これを飼育槽に給餌している。そして、従来、飼料となる植物プランクトンの培養については、栄養、空気、光熱の面で、その調整が難しいことから、熟練者による手作業に頼っていた。また、水棲生物飼育用の水槽として、特開平１−３０９６３０号で知られた生物飼育環境を維持するものがある。 When cultivating shellfish and crustaceans in breeding tanks, phytoplankton (diatoms) as feed for them is cultured in other cultivation equipment and fed to the breeding tanks. Conventionally, the cultivation of phytoplankton, which is used as feed, has been dependent on manual work by skilled workers because it is difficult to adjust nutrients, air and light and heat. As an aquarium for breeding aquatic organisms, there is an aquarium that maintains the breeding environment known in Japanese Patent Application Laid-Open No. 1-309630.

特開平１−３０９６３０号公報JP-A-1-309630

　微生物培養システムの培養槽に空気を供給する構成において、培養槽より開閉弁までの排気管内が攪拌されて淀みが生じず、雑菌発生の恐れがなくなるように構成するものである。
　更に培養槽内の培養液も攪拌されて、呼吸や光合成促進に寄与するようにするものである。
　そして、従来のように、培養液内に浸漬するような空気管は削除されるので、洗浄不良の事態が解消される。これらにより、培養不良が低減して、飼料の安定生産が可能となる。
　また、空気管の開閉弁を通電時に閉弁する構成として、非通電時の平常時に空気が常に培養槽に供給されて、攪拌作用を及ぼすものであり、停電等の異常時にもこの空気供給が確保されるので、安全性があり、また、平常時が非通電時であるから、コスト安となるように構成するものである。 In the configuration in which air is supplied to the culture tank of the microorganism culture system, the inside of the exhaust pipe from the culture tank to the on-off valve is agitated so that stagnation does not occur and there is no possibility of generation of various bacteria.
Further, the culture solution in the culture tank is also stirred so as to contribute to respiration and photosynthesis.
Further, unlike the related art, the air pipe that is immersed in the culture solution is eliminated, so that the situation of poor cleaning is eliminated. With these, poor culture is reduced, and stable production of feed becomes possible.
In addition, air is always supplied to the culture tank at normal times when power is not supplied to the culture tank so that the air pipe open / close valve closes when power is supplied. Therefore, safety is ensured, and the cost is reduced because the power is not supplied during normal times.

　本発明は、以上のような問題を解決するために、次のような手段を用いるものである。
　請求項１においては、微生物と栄養液を調整混入した培養液を培養槽内に入れ、光熱、空気を供給して微生物を培養し、該培養槽内の培養液を、他の生物の育成槽内に餌として付与する一方、その一部を培養液への混入用として採出する微生物培養システムにおいて、該培養槽の底部より垂設される排出管内における開閉弁直上部に、微生物培養用の空気を供給するように構成したものである。 The present invention uses the following means to solve the above problems.
In Claim 1, a culture solution prepared by mixing and mixing a microorganism and a nutrient solution is placed in a culture tank, and light and heat are supplied to culture the microorganisms. In a microbial culture system, a part of which is fed as a bait, and a part of which is taken out for incorporation into a culture solution, a microbial culture system is provided immediately above an on-off valve in a discharge pipe vertically provided from the bottom of the culture tank. It is configured to supply air.

　請求項２においては、請求項１記載の微生物培養システムにおいて、該空気の供給管に、開閉弁を介設し、通電時に閉弁する構成としたものである。 According to a second aspect of the present invention, in the microorganism culture system according to the first aspect, an on-off valve is provided in the air supply pipe, and the valve is closed when power is supplied.

　このような微生物培養システムにおいて、培養槽においては、空気を、培養中に淀みの生じやすい排出管内の開閉弁直上部に供給するようにすることで、この部位が攪拌され、更に培養槽内の培養液全体を攪拌し、雑菌の生じるような淀みを生じさせない。 In such a microorganism culture system, in the culture tank, the air is supplied to the portion immediately above the on-off valve in the discharge pipe where stagnation is likely to occur during the culture, so that this portion is stirred, and furthermore, the inside of the culture tank is The whole culture solution is agitated so as not to cause stagnation such as germs.

　また、空気管は通電時に閉弁する、即ち、平常時の非通電時には、開弁して、培養槽に空気を送り込む構成としているので、異常による停電時にも空気が培養槽に送り込まれ、攪拌作用をもたらして、培養不良を解消する。 In addition, the air tube is closed when energized, that is, it is opened during normal de-energization and air is sent to the culture tank. It works to eliminate poor culture.

　本発明は、以上のように構成したので、次のような効果を奏する。
　微生物培養システムにおいて、請求項１の如く培養槽に空気供給するよう構成したので、培養槽より開閉弁までの排気管内が攪拌されて淀みが生じず、雑菌発生の恐れがなくなる。更に培養槽内の培養液も攪拌されて、呼吸や光合成促進に寄与する。そして、従来のように、培養液内に浸漬するような空気管は削除されるので、洗浄不良の事態が解消される。これらにより、培養不良が低減して、飼料の安定生産が可能となる。 Since the present invention is configured as described above, the following effects can be obtained.
In the microorganism culturing system, since the air is supplied to the culturing tank as in claim 1, the inside of the exhaust pipe from the culturing tank to the on-off valve is agitated, so that stagnation does not occur, and there is no risk of generating bacteria. Further, the culture solution in the culture tank is also stirred, which contributes to respiration and photosynthesis. Further, unlike the related art, the air pipe that is immersed in the culture solution is eliminated, so that the situation of poor cleaning is eliminated. With these, poor culture is reduced, and stable production of feed becomes possible.

　また、請求項２の如く、空気管の開閉弁を通電時に閉弁する構成としたので、非通電時の平常時に空気が常に培養槽に供給されて、攪拌作用を及ぼすものであり、停電等の異常時にもこの空気供給が確保されるので、安全性があり、また、平常時が非通電時であるから、コスト安となる。 Further, since the opening and closing valve of the air pipe is closed when energized as in claim 2, air is always supplied to the culture tank during non-energized conditions and exerts a stirring action. In this case, the air supply is ensured even in the event of an abnormality, so that there is safety, and the cost is reduced because the power is not supplied during normal times.

　本発明の実施例を、添付の図面を基に説明する。図１は本発明の微生物培養システムのシステムブロック図、図２は同じくシステム配管図、図３は培養槽の側面図である。 An embodiment of the present invention will be described with reference to the accompanying drawings. FIG. 1 is a system block diagram of a microorganism culturing system of the present invention, FIG. 2 is a system piping diagram, and FIG. 3 is a side view of a culture tank.

　本発明の微生物培養システムの全体の流れについて、図１及び図２により説明する。この実施例においては、飼育槽内の海水にて飼育する浅蜊等の貝類等の飼料としての植物プランクトン（珪藻Ｍ）、例えば、キートセロス・グラシリスやパブロバ等を培養する。まず、図２図示の培養液調整槽７にて培養液を調整混合する行程（図１図示の＜５＞培養液調整）について説明すると、培養液の主液となる海水ＳＷは、海水供給管１を通してフィルター２で濾過し（＜１＞海水精密濾過）、紫外線３にて殺菌して（＜２＞海水ＵＶ（紫外線）殺菌）、培養液調整槽７内に供給する。また、培養に必要な栄養塩ＶＬの原液を注入ビン５内に入れ、調整して保冷室４内（例えば５℃）にて貯蔵しており（＜３＞栄養塩調整）、必要分の栄養塩ＶＬを、栄養塩供給管６を介して培養液調整槽７内に供給する。　　　 全体 The overall flow of the microorganism culturing system of the present invention will be described with reference to FIGS. In this embodiment, phytoplankton (diatom M) as a feed for shellfish such as Asari, which is bred in seawater in a breeding tank, such as Quitocellos gracilis and Pavlova, is cultured. First, the process of adjusting and mixing the culture solution in the culture solution adjusting tank 7 shown in FIG. 2 (<5> adjustment of the culture solution in FIG. 1) will be described. The mixture is filtered through 1 through a filter 2 (<1> seawater microfiltration), sterilized by ultraviolet rays 3 (<2> sterilized by seawater UV (ultraviolet rays)), and supplied into a culture solution adjusting tank 7. In addition, a stock solution of nutrient VL necessary for culture is put into the injection bottle 5, adjusted and stored in the cold room 4 (for example, at 5 ° C.) (<3> nutrient adjustment), and nutrient required The salt VL is supplied through the nutrient supply pipe 6 into the culture solution adjusting tank 7.

　そして、培養すべき珪藻Ｍは、後の＜７＞培養行程、或いは＜８＞収穫行程において、一部採出したものであり（＜４＞植え継ぎ）、これを培養液調整槽７内に供給する。こうして殺菌海水ＳＷ、栄養塩ＶＬ、珪藻Ｍを培養液調整槽７内にて混合し、ｐＨコントローラー８にてｐＨ調整をしながら培養液を調整する。 The diatom M to be cultured is partially extracted in the subsequent <7> culturing step or <8> harvesting step (<4> subculture), and is placed in the culture solution adjusting tank 7. Supply. Thus, the sterilized seawater SW, nutrient VL, and diatom M are mixed in the culture solution adjusting tank 7 and the pH of the culture solution is adjusted by the pH controller 8 to adjust the culture solution.

　培養液調整槽７内にて調整した培養液ＣＬは、供給排出管９にて、保冷室内（例えば１０℃〜２０℃）の培養槽１０・１０・・・に底面１０ｄに開口した供給排出管９の管端開口９ｃを介して供給される（＜６＞培養槽調整）。培養槽１０・１０・・・には、蛍光灯１１より珪藻Ｍの光合成に必要な光と熱を供給し、また、空気管１２を供給排出管９内に導入して培養槽１０・１０・・・内の培養液内に攪拌混入させ、珪藻Ｍ培養のための空気Ａの供給を底面１０ｄに開口した供給排出管９の管端開口９ｃから浮上させるようにして行っている（＜７＞培養）。 The culture solution CL adjusted in the culture solution adjusting tank 7 is supplied to the supply / discharge tube 9 and supplied to the culture tanks 10, 10... In a cold room (for example, 10 ° C. to 20 ° C.). 9 is supplied through a tube end opening 9c (<6> Adjustment of culture tank). Are supplied with light and heat necessary for photosynthesis of the diatom M from the fluorescent lamp 11, and an air pipe 12 is introduced into the supply / discharge pipe 9 to the culture tanks 10. ··· The mixture is stirred and mixed into the culture solution inside, so that the supply of air A for diatom M culture is made to float from the tube end opening 9c of the supply / discharge tube 9 opened on the bottom surface 10d (<7>). culture).

　こうして珪藻Ｍが培養されて（例えば、６日間）、該培養槽１０内の培養液ＣＬが、飼料として供給可能な状態となると、該培養液ＣＬは、飼料Ｆとして、供給排出管９を介して、飼料槽１３内に供給されて一定量貯蔵され（＜８＞収穫）、給餌管１５を介して、貝類を飼育する飼育槽１６・１６・・・に供給される（＜９＞給餌）。なお前記の如く、＜８＞収穫（＜７＞培養）行程中において、供給排出管９を介して培養液調整槽７内に珪藻Ｍの一部を供給して、＜４＞植え継ぎとするが、そのため、飼料槽１６より培養液調整槽７に植え継ぎ管１７が配管されている。該培養液調整槽７及び該飼料槽１３に対しては、各々、空気管１８・１９により空気Ａの供給が行われるものであり、該培養液調整槽７へは該供給排出管９に、該飼料槽１３へはドレン管１４に各々空気導入して供給している。　　　 When the diatom M is thus cultured (for example, for 6 days) and the culture liquid CL in the culture tank 10 is ready to be supplied as feed, the culture liquid CL is supplied as feed F via the supply / discharge pipe 9. Are fed into the feed tank 13 and stored in a certain amount (<8> harvest), and are supplied to the breeding tanks 16, 16... For breeding shellfish through the feeding pipe 15 (<9> feeding) . As described above, during the <8> harvesting (<7> culture) process, part of the diatom M is supplied into the culture solution adjusting tank 7 via the supply / discharge pipe 9, and <4> subculture is performed. However, a connection pipe 17 is provided from the feed tank 16 to the culture solution adjusting tank 7. The air A is supplied to the culture solution adjusting tank 7 and the feed tank 13 by air pipes 18 and 19, respectively. The feed tank 13 is supplied with air introduced into a drain pipe 14.

　培養液調整槽７、培養槽１０、飼料槽１３、及び供給排出管９には、洗浄原液タンク２０及び受水槽２１より合流する洗浄液管２２により、洗浄液ＷＬを供給して、内部洗浄可能となっている（＜１０＞水槽洗浄）。該洗浄原液タンク２０内には洗浄原液の次亜塩素酸ナトリウム溶液を、受水槽２１内には水道水Ｗが供給されて、水道水Ｗを貯蔵するようになっており、これらを洗浄液管２２内に導入し、洗浄液ＷＬとして各部に供給するのであるが、培養液調整槽７、培養槽１０、及び飼料槽１３に対しては、管端にノズルが付設されていて、上部より洗浄液ＷＬが噴射される構成となっている。供給排出管９内は、洗浄液ＷＬを通過させることで洗浄するものである。 The cleaning liquid WL is supplied to the culture liquid adjusting tank 7, the culture tank 10, the feed tank 13, and the supply / discharge pipe 9 by the cleaning liquid pipe 22 which joins from the cleaning raw liquid tank 20 and the water receiving tank 21, so that the inside can be cleaned. (<10> tank cleaning). The washing stock solution tank 20 is supplied with a washing stock solution of sodium hypochlorite solution, and the receiving tank 21 is supplied with tap water W to store the tap water W. Is introduced into each part and supplied as a washing liquid WL to each part. For the culture liquid adjusting tank 7, the culture tank 10, and the feed tank 13, a nozzle is provided at a pipe end, and the washing liquid WL is supplied from above. It is configured to be injected. The inside of the supply / discharge pipe 9 is cleaned by passing the cleaning liquid WL.

　以上のように珪藻を培養、給餌し、また、水槽、配管洗浄される微生物培養システムにおいて、各配管には電磁バルブや調圧バルブ等の各種バルブが介設されており、これらのバルブを、各種センサーやコントローラーを基に自動開閉制御して、海水ＳＷ、栄養塩ＶＬ、培養液ＣＬ、飼料Ｆ、空気Ａ、洗浄液ＷＬの供給、排出量を自動調節可能となっている。また、海水供給管１、栄養塩供給管６、給餌管１５、植え継ぎ管１７、及び洗浄原液タンク２０出口、受水槽２１出口には定量ポンプＰ（ダイヤフラムポンプ等）が介設されていて、定量の海水ＳＷ、栄養塩ＶＬ、飼料Ｆ（植え継ぎ用のものを含む。）、洗浄原液、水道水Ｗを通過可能となっている。なお、図１に示す如く、栄養塩の調整作業は手作業であり、また、植え継ぎは、図２に図示するように、定量ポンプＰにより自動制御できるが、手作業にて行ってもよいものとしている。 In the microorganism culture system in which diatoms are cultured and fed as described above, and the water tank and the pipes are washed, various valves such as an electromagnetic valve and a pressure regulating valve are interposed in each pipe, and these valves are used. The supply and discharge of seawater SW, nutrient VL, culture solution CL, feed F, air A, and washing solution WL can be automatically adjusted by automatic opening / closing control based on various sensors and controllers. A fixed amount pump P (diaphragm pump or the like) is provided at the seawater supply pipe 1, the nutrient supply pipe 6, the feed pipe 15, the planting pipe 17, the outlet of the washing stock solution tank 20, and the outlet of the water receiving tank 21. A fixed amount of seawater SW, nutrients VL, feed F (including those for planting), undiluted washing solution, and tap water W can pass through. In addition, as shown in FIG. 1, the adjustment work of the nutrient salt is manual work, and the planting can be automatically controlled by the metering pump P as shown in FIG. 2, but may be performed manually. It is assumed.

　以上のような構成及び流れの微生物培養システムにおいて、各部の構成について説明する。まず、供給排出管９について説明する。図２の如く、培養液調整槽７、各培養槽１０、及び飼料槽１３の相互間においては、培養液ＣＬ、飼料Ｆの供給、排出を同一の供給排出管９にて行うようにしている。また、配設高さを、図２の如く、培養液調整槽７を最上位に、培養槽１０を上下中間位に、飼料槽１３を最下位としている。従って、供給排出管９において、バルブＶ１、バルブＶ２・Ｖ２・・・を開弁し、バルブＶ３を閉弁すれば、培養液調整槽７より各培養槽１０に培養液ＣＬを供給できる。 構成 The configuration of each part in the microorganism culture system having the above configuration and flow will be described. First, the supply / discharge pipe 9 will be described. As shown in FIG. 2, the supply and discharge of the culture solution CL and the feed F are performed by the same supply / discharge pipe 9 between the culture solution adjusting tank 7, each of the culture tanks 10, and the feed tank 13. . Also, as shown in FIG. 2, the arrangement height is such that the culture solution adjusting tank 7 is at the highest position, the culture tank 10 is at an intermediate position between the top and bottom, and the feed tank 13 is at the lowest position. Therefore, when the valves V1, V2, V2,... Are opened and the valve V3 is closed in the supply / discharge pipe 9, the culture medium CL can be supplied from the culture medium adjustment tank 7 to each of the culture tanks 10.

　この時、各培養槽１０への分岐管９ａにおいては、培養液ＣＬが、図２中の矢印の方向に流れる。そして、バルブＶ１を閉弁し、バルブＶ２・Ｖ２・・及びバルブＶ３を開弁すれば、各培養槽１０より飼料槽１３への飼料Ｆの供給が行われる。この時、該分岐管９ａにおいては、飼料Ｆが矢印の方向に流れる。なお、バルブＶ４は、通常時において閉弁しており、このバルブを開弁して、他のバルブを閉弁すれば、管内の培養液ＣＬ又は飼料Ｆをドレンできる。このように、バルブの開閉操作によって、供給と排出を同一の管にて行うことができ、配管コストの節約や操作の容易化が実現できる。 At this time, the culture solution CL flows in the direction of the arrow in FIG. 2 in the branch pipe 9a to each culture tank 10. When the valve V1 is closed and the valves V2, V2,... And the valve V3 are opened, the feed F is supplied from each culture tank 10 to the feed tank 13. At this time, the feed F flows in the direction of the arrow in the branch pipe 9a. The valve V4 is normally closed, and if this valve is opened and other valves are closed, the culture liquid CL or the feed F in the tube can be drained. In this manner, by opening and closing the valve, supply and discharge can be performed in the same pipe, so that piping cost can be saved and operation can be simplified.

　次に、培養槽１０の構成について、図３より説明する。まず、培養液ＣＬ内への空気供給構成について説明すると、前記の如く、各培養槽１０内の培養液ＣＬ内への空気供給は、空気管１２より供給排出管９に導入するものとなっており、該空気管１２からの導入部位は、各培養槽１０の底部に繋がる分配管９ｂ・９ｂ・・・に介設するバルブＶ２・Ｖ２・・・の直上部、即ち、該培養槽１０の底部の直下部となっている。 Next, the configuration of the culture tank 10 will be described with reference to FIG. First, the configuration of the air supply into the culture solution CL will be described. As described above, the air supply into the culture solution CL in each culture tank 10 is introduced from the air pipe 12 to the supply / discharge pipe 9. The introduction site from the air pipe 12 is located immediately above the valves V2, V2,... Provided on the distribution pipes 9b, 9b,. It is directly below the bottom.

　この部位に空気導入した理由について説明する。従来は、培養槽１０内には、上部より空気管（ガラス管、或いは、エアストンを付設したもの）を導入し、その管端を培養液ＣＬ内に浸していたのであり、即ち、該空気管より直接的に培養槽１０内の培養液ＣＬ内に空気導入する構成していたのである。しかし、この場合には、培養槽１０内の洗浄時において、培養液ＣＬ内に浸漬される空気管の裏側（矢印の指す部分）が洗浄されにくく、洗浄不足を引き起こす。洗浄不足は、雑菌発生の原因となり、培養に悪影響を及ぼす。供給排出管９内に空気導入する構成にすれば、培養槽１０内の培養液ＣＬ内に空気管が浸漬することがなくなるので、洗浄不足の事態を解消する。 理由 Explain why air was introduced into this area. Conventionally, an air tube (a glass tube or a tube provided with an airstone) was introduced into the culture tank 10 from above, and the end of the tube was immersed in the culture solution CL. Thus, air was introduced into the culture solution CL in the culture tank 10 more directly. However, in this case, when the inside of the culture tank 10 is washed, the back side (portion indicated by the arrow) of the air tube immersed in the culture solution CL is difficult to be washed, which causes insufficient washing. Insufficient washing causes the occurrence of various bacteria and adversely affects the culture. With a configuration in which air is introduced into the supply / discharge tube 9, the air tube is not immersed in the culture solution CL in the culture tank 10, so that the situation of insufficient washing is eliminated.

　そして、前記の如く、培養槽１０底部の下方における分配管９ｂのバルブＶ２上部に導入したのは、もし、この部位に空気導入しなければ、培養中においてこの部位に淀みが生じる。そこから、雑菌、硫化水素等の有害物質が発生し、培養液ＣＬ内において、酸欠状態を引き起こして、培養に悪影響を与える。そこでこの部位に空気導入することで、この部位における培養液ＣＬは攪拌されて、淀みが生じなくなるのである。従って、空気導入箇所も、空気は分配管９ｂ内に導入された後、上方に移動するので、最下部のバルブＶ２の直上部にすれば、淀みを生じる箇所が皆無となる。この構成は、培養液調整槽７及び飼料槽１３においても応用されている。 As described above, the introduction into the upper part of the valve V2 of the distribution pipe 9b below the bottom of the culture tank 10 causes stagnation in this part during culture if air is not introduced into this part. From there, harmful substances such as germs and hydrogen sulfide are generated, causing an oxygen-deficient state in the culture solution CL, which adversely affects the culture. Therefore, by introducing air into this site, the culture solution CL at this site is agitated and stagnation does not occur. Accordingly, since the air is introduced upward into the distribution pipe 9b after the air is introduced into the distribution pipe 9b, if the air is introduced directly above the lowermost valve V2, there is no place where stagnation occurs. This configuration is also applied to the culture solution adjusting tank 7 and the feed tank 13.

　次に、培養槽１０の蓋１０ａの構成について説明する。蓋１０ａは取外し容易に施蓋されるものであり、透明としておくことで、内部の培養液ＣＬの様子が外部より観察容易となっている。そして、洗浄液管２２より連接される洗浄ホース２２ａの管端を固設して、ノズル２３を蓋１０ａの下部に垂設している。可撓性のあるホースとすることで、蓋１０ａの取外しが自由となっている。 Next, the configuration of the lid 10a of the culture tank 10 will be described. The lid 10a is easily removed and covered, and by keeping it transparent, the state of the culture solution CL inside can be easily observed from outside. Then, a pipe end of a cleaning hose 22a connected to the cleaning liquid pipe 22 is fixedly provided, and a nozzle 23 is vertically provided below the lid 10a. By using a flexible hose, the lid 10a can be freely removed.

　該ノズル２３は回転可能となっていて、回転して洗浄液を噴射することにより、培養槽１０内の洗浄を良好に行うことができるようになっており、蓋をした状態のまま培養槽１０内の洗浄が可能なのであり、また、一々洗浄の度に洗浄用のノズルを培養槽１０内に配設する必要がなく、洗浄液管２２内に洗浄液ＷＬを供給すれば、自然にノズルより洗浄液が噴射されて槽内洗浄がなされるのである。この構成は、該培養液調整槽７及び該飼料槽１３にも応用されるものとする。更に、該蓋１０ａには、排気口１０ｂが穿設されており、また、培養槽１０と蓋１０ａとの間に隙間１０ｃを生じさせる構成とすることも可能であり、前記の如く、分配管９ｂより導入された空気が培養槽１０内の培養液ＣＬ内に混入された後、珪藻Ｍの呼吸や光合成等により、培養液ＣＬ上面より排気されるが、この排気を排気口１０ｂより排気して、呼吸及び光合成を活性化することができるのである。 The nozzle 23 is rotatable, and the nozzle 23 is rotated so as to spray the cleaning liquid so that the inside of the culture tank 10 can be washed well. It is not necessary to arrange a washing nozzle in the culture tank 10 for each washing, and if the washing liquid WL is supplied into the washing liquid pipe 22, the washing liquid is spouted from the nozzle naturally. Then, the inside of the tank is cleaned. This configuration is also applied to the culture solution adjusting tank 7 and the feed tank 13. Further, an exhaust port 10b is formed in the lid 10a, and a gap 10c may be formed between the culture tank 10 and the lid 10a. After the air introduced from 9b is mixed into the culture solution CL in the culture tank 10, the air is exhausted from the upper surface of the culture solution CL by respiration or photosynthesis of the diatom M. This exhaust is exhausted from the exhaust port 10b. Thus, respiration and photosynthesis can be activated.

　その他、培養槽１０内の培養液ＣＬ（飼料Ｆ）は、底部に繋がる分配管９ｂにて供給排出されるので、該培養槽１０の底面１０ｄをテーパー状として、液の流動を円滑化し、排出後には培養槽１０内に液が残留しにくい構成としている。なお、蛍光灯１１は側面より照射し、培養槽１０内に広く光を届かせるようにし、更に蛍光灯１１をタイマーにて明暗制御し、培養活性化を図っている。 In addition, since the culture liquid CL (feed F) in the culture tank 10 is supplied and discharged through the distribution pipe 9b connected to the bottom, the bottom surface 10d of the culture tank 10 is tapered to smooth the flow of the liquid and discharge. The structure is such that the liquid hardly remains in the culture tank 10 later. The fluorescent lamp 11 is illuminated from the side to make the light reach the inside of the culture tank 10 broadly, and the brightness of the fluorescent lamp 11 is controlled by a timer to activate the culture.

　次に、空気管１２においては、電磁バルブＶ５を介設しているが、平時の非通電時に開弁状態とし、通電時に閉弁状態としている。即ち、通常時は培養槽１０や培養液調整槽７、及び飼料槽１３に空気を送り込んで、培養液ＣＬ又は飼料Ｆへの空気供給をして、呼吸、更に攪拌による淀み解消に供しており、洗浄時等の限られた場合のみ空気供給を停止する構成として、コスト低下を実現できるのであり、もしも停電等で電磁バルブＶ５が非通電状態となった場合には、開弁状態で空気供給がなされるので、培養槽１０等における培養槽ＣＬや飼料Ｆの安全性が保持できるのである。 Next, in the air pipe 12, although the electromagnetic valve V5 is interposed, the valve is opened when power is not supplied in normal times and closed when power is supplied. That is, normally, air is fed into the culture tank 10, the culture liquid adjustment tank 7, and the feed tank 13 to supply air to the culture liquid CL or the feed F, and is used for breathing and further eliminating stagnation by stirring. The cost can be reduced by stopping the air supply only in a limited case such as at the time of cleaning, and if the solenoid valve V5 is de-energized due to a power failure or the like, the air supply is stopped in the open state. Therefore, the safety of the culture tank CL and the feed F in the culture tank 10 and the like can be maintained.

　また、空気管１１内には、通常は呼吸用のＯ2 を供給するが、光合成用や、ｐＨ調整のために、ＣＯ2 を供給することも可能である。その他、空気管１１及び空気管１８・１９に洗浄液ＷＬを供給可能とし（図２には図示せず）、管内洗浄ができる構成としている。また、フィルターを介設して、空気清浄化を図っている。 In addition, although O 2 for breathing is usually supplied into the air pipe 11, CO 2 can be supplied for photosynthesis or for pH adjustment. In addition, the cleaning liquid WL can be supplied to the air pipe 11 and the air pipes 18 and 19 (not shown in FIG. 2), so that the inside of the pipe can be cleaned. In addition, air is cleaned by interposing a filter.

本発明の微生物培養システムのシステムブロック図である。It is a system block diagram of the microorganism culture system of the present invention. 同じくシステム配管図である。It is a system piping diagram similarly. 培養槽の側面図である。It is a side view of a culture tank.

符号の説明Explanation of reference numerals

　Ｍ　　　珪藻
　ＳＷ　　海水
　ＶＬ　　栄養塩
　ＣＬ　　培養液
　Ｆ　　　飼料
　Ａ　　　空気
　Ｗ　　　水道水
　ＷＬ　　洗浄液
　Ｖ１〜Ｖ５　バルブ（電磁バルブ）
　Ｐ　　　定量ポンプ
　７　　　培養液調整槽
　９　　　供給排出管
　９ａ　　分岐管
　９ｂ　　分配管
　１０　　培養槽
　１０ａ　蓋
　１０ｂ　排気口
　１１　　蛍光灯
　１２　　空気管
　１３　　飼料槽
　１６　　飼育槽
　１７　　植え継ぎ管
　２０　　洗浄原液タンク
　２１　　受水槽
　２２　　洗浄液管
　２２ａ　洗浄ホース
　２３　　洗浄ノズル M Diatom SW Seawater VL Nutrient CL Culture solution F Feed A Air W Tap water WL Wash solution V1-V5 Valve (electromagnetic valve)
P metering pump 7 culture solution adjusting tank 9 supply / discharge pipe 9a branch pipe 9b distribution pipe 10 culture tank 10a lid 10b exhaust port 11 fluorescent lamp 12 air pipe 13 feed tank 16 breeding tank 17 planting pipe 20 washing stock solution tank 21 water receiving tank 22 Cleaning liquid tube 22a Cleaning hose 23 Cleaning nozzle

Claims (2)

　微生物と栄養液を調整混入した培養液を培養槽内に入れ、光熱、空気を供給して微生物を培養し、該培養槽内の培養液を、他の生物の育成槽内に餌として付与する一方、その一部を培養液への混入用として採出する微生物培養システムにおいて、該培養槽の底部より垂設される排出管内における開閉弁直上部に、微生物培養用の空気を供給することを特徴とする微生物培養システム。 A culture solution prepared by mixing and mixing a microorganism and a nutrient solution is put into a culture tank, and light and heat are supplied to culture the microorganism, and the culture solution in the culture tank is fed as a feed to a growth tank for other organisms. On the other hand, in a microorganism culturing system in which a part of the microorganism is taken out for mixing into a culture solution, air for microorganism culturing is supplied just above an on-off valve in a discharge pipe vertically provided from the bottom of the culture tank. Characterized microorganism culture system. 　請求項１記載の微生物培養システムにおいて、該空気の供給管に、開閉弁を介設し、通電時に閉弁する構成としたことを特徴とする微生物培養システム。 A microbial culture system according to claim 1, wherein an on-off valve is interposed in the air supply pipe, and the valve is closed when power is supplied.
JP2003334024A 2003-09-25 2003-09-25 Microbial culture system Expired - Lifetime JP4245448B2 (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITPD20090141A1 (en) * 2009-05-19 2010-11-20 Microlife S R L BIOREACTOR FOR THE PRODUCTION OF PHOTOSYNTHETIC MICRO-ORGANISMS IN LIQUID CULTURE
JP2018537948A (en) * 2015-12-23 2018-12-27 コリア ディストリクト ヒーティング コーポレーションＫｏｒｅａ Ｄｉｓｔｒｉｃｔ Ｈｅａｔｉｎｇ Ｃｏｒｐ． Multi-function port for cultivation and collection of microalgae

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITPD20090141A1 (en) * 2009-05-19 2010-11-20 Microlife S R L BIOREACTOR FOR THE PRODUCTION OF PHOTOSYNTHETIC MICRO-ORGANISMS IN LIQUID CULTURE
JP2018537948A (en) * 2015-12-23 2018-12-27 コリア ディストリクト ヒーティング コーポレーションＫｏｒｅａ Ｄｉｓｔｒｉｃｔ Ｈｅａｔｉｎｇ Ｃｏｒｐ． Multi-function port for cultivation and collection of microalgae

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