CN109576133A - A kind of microbial bacterial agent culture apparatus and cultural method - Google Patents
A kind of microbial bacterial agent culture apparatus and cultural method Download PDFInfo
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- CN109576133A CN109576133A CN201811604770.1A CN201811604770A CN109576133A CN 109576133 A CN109576133 A CN 109576133A CN 201811604770 A CN201811604770 A CN 201811604770A CN 109576133 A CN109576133 A CN 109576133A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/50—Means for positioning or orientating the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
Abstract
The present invention provides a kind of microbial bacterial agent culture apparatus and cultural methods.The microbial bacterial agent culture apparatus includes activation of microorganism culture tank and electric automatic control cabinet；The dissolved oxygen electrode of temperature electrode, the pH electrode and test dissolved oxygen content of testing pH in the activation of microorganism culture tank equipped with test temperature, the output end of the temperature electrode, pH electrode and dissolved oxygen electrode access the electric automatic control cabinet.Microbial bacterial agent culture apparatus and cultural method provided by the invention, can effectively reduce environment water it is biological prosthetic during microbial inoculum dosage, solve microbial inoculum transport and storage caused by spawn activity decline, the reaction time it is longer and play effect it is slow the problems such as.
Description
Technical field
The present invention relates to microorganism Water Ecological Recovery field, more particularly, to a kind of microbial bacterial agent culture apparatus and
Cultural method.
Background technique
As water pollution is increasingly severe, the consciousness that people protect Ecology is significantly increased, river system repair technology
It comes into being under these conditions, mainstream water ecology recovery technique mainly includes physical, chemical and biological methods at present.
It can efficiently be completed at normal temperatures and pressures as the bioremediation technology to effect a permanent cure, this method repairs level from water ecology
It sets out, it is intended to by the culture of microorganism, plant and aquatic animal, be disliked for pollution caused by anthropogenic discharge in water body and environment
Change collapse, realize the elimination of pollutant and keep water stabilization, fundamentally gradually restore the self-purification capacity of environment, maintains good
Property ecological circulation.In biological renovation method, microbial technique is even more to have apparent application advantage: its source is wide, breeding is fast,
It is strong to environmental suitability；Each type organic and eutrophication nitrogen and phosphorus element can not only be removed, moreover it is possible to remove stink, reduce color
Degree, inhibition growth of pathogenic bacteria etc.；Processing method is mild, and the influence to environment is small, does not generate secondary pollution.
But microorganism remediation technology has dosage larger, bacteria concentration and quantity are not easy to keep in water body, and microbial inoculum saves
And vigor caused by transport declines, it is difficult to reach pollutant removal design object, cost of labor is larger.
Summary of the invention
The present invention is larger for dosage present in the reparation of microorganism water ecology, and microbial inoculum is living caused by saving and transporting
Power declines and is difficult to reach processing target, needs the problems such as culture in real time, provides a kind of microbial bacterial agent culture apparatus, and phase
A kind of cultural method should be proposed, the semicontinuous culture of microbial inoculum may be implemented, operation is automatic simple, time saving and energy saving.
The microbial bacterial agent culture apparatus, including activation of microorganism culture tank and electric automatic control cabinet；The microorganism
The dissolved oxygen electrode of temperature electrode, the pH electrode and test dissolved oxygen content of testing pH in activation culture tank equipped with test temperature, institute
The output end for stating temperature electrode, pH electrode and dissolved oxygen electrode accesses the electric automatic control cabinet.
Preferably, it is additionally provided with temperature control heating pipe and/or heat exchanging water pipe in the activation of microorganism culture tank, the temperature control adds
Heat pipe accesses the electric automatic control cabinet；It the water inlet end of the heat exchanging water pipe and is located at outside the activation of microorganism culture tank
Exchange heat water pump be connected, the electric automatic control cabinet according to the temperature electrode output control it is described heat exchange water pump unlatching and
It closes.
Preferably, the microbial bacterial agent culture apparatus further include the acid & alkali liquid that is connected with the activation of microorganism culture tank into
Material pump, the acid & alkali liquid feed pump access the electric automatic control cabinet, and the electric automatic control cabinet is according to the pH electrode
Control unlatching and closing for the acid & alkali liquid feed pump.
Preferably, aerator is additionally provided in the activation of microorganism culture tank, the aerator passes through pipeline and sets
Air pump outside the activation of microorganism culture tank is connected, and the air pump is connected with the electric automatic control cabinet, described
Electric automatic control cabinet controls unlatching and closing for the air pump according to the output of the dissolved oxygen electrode.
Preferably, the top of the activation of microorganism culture tank is equipped with illumination apparatus, the electric automatic control cabinet control
The illumination apparatus unlatches and closes.
Preferably, the top of the activation of microorganism culture tank is connected with intake pump, and the intake pump is by being located at the electricity
Timing control system control in gas automatic control cabinet.
Preferably, the bottom of the activation of microorganism culture tank is connected by going out water management high-pressure hydraulic pump with flusher,
The water management high-pressure hydraulic pump out is controlled by the electric automatic control cabinet.
Preferably, the flusher is located at outside the activation of microorganism culture tank, solid using the fixed bracket of rotary type
It is fixed；The flusher includes sprinkling outlet pipe and electromagnetic flow valve interconnected, and the outlet pipe passes through electromagnetic flow valve
It is connected with the water management high-pressure hydraulic pump out.
It preferably, further include for displays temperature value, pH value and oxygen dissolving value instrument number in the electric automatic control cabinet
According to display platform.
Preferably, the activation of microorganism culture tank and electric automatic control cabinet are placed in box protection cabinets, described box
The top and front of protection cabinets are equipped with opening and removable door corresponding with the opening.
The present invention also provides a kind of methods of microbial bacterial agent culture, and this method comprises the following steps:
1) nutriment necessary to growth is added in activation of microorganism culture tank；
2) water of volume needed for being added in Xiang Suoshu activation of microorganism culture tank；
3) strain is seeded in the activation of microorganism culture tank with required inoculum concentration, by being arranged in the microorganism
Optimal culture condition, activation culture is arranged in temperature electrode, pH electrode and dissolved oxygen electrode inside activation culture tank.
Compared with prior art, the invention has the benefit that
Microbial bacterial agent culture apparatus and cultural method provided by the invention, it is biological prosthetic can to effectively reduce environment water
Microbial inoculum dosage in the process, spawn activity decline caused by solving microbial inoculum transport and storing, the reaction time is longer and plays effect
Semicontinuous culture and the automatic spraying of microbial inoculum preferably may be implemented in the problems such as fruit is slow, saves artificial and machine cost,
Lifting construction security intensity.
Detailed description of the invention
It, below will be to use required in description of the prior art in order to illustrate more clearly of the technical solution of this art inventions
Attached drawing make one simply introduce, it should be apparent that, the accompanying drawings in the following description is some currently preferred embodiments of the present invention, for
For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings other
Attached drawing.
Fig. 1 is the microbial bacterial agent culture apparatus schematic diagram provided in a preferred embodiment of the invention；
Wherein, 1, solar panel；2, box protection cabinets；3, the fixed bracket of packaged type；4, activation of microorganism culture
Tank；5, electric automatic control cabinet；6, temperature control heating pipe；7, natural light lamp；8, microporous aeration disc；9, temperature electrode；10, pH electricity
Pole；11, dissolved oxygen electrode；12, high pressure sprinkler；13, outlet pipe；14, inlet pipeline；15, acid solution feed pipe；16, lye is fed
Pipe.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is
A part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art
Every other embodiment obtained without creative efforts, shall fall within the protection scope of the present invention.
The present invention provides a kind of microbial bacterial agent culture apparatus, including activation of microorganism culture tank and electric automatic control
Cabinet；The pH electrode and test dissolved oxygen content of temperature electrode, test pH in the activation of microorganism culture tank equipped with test temperature
Dissolved oxygen electrode, the output end of the temperature electrode, pH electrode and dissolved oxygen electrode accesses the electric automatic control cabinet.In reality
In the operating process of border, nutriment necessary to microbial bacterial agent is grown is added in activation of microorganism culture tank, Xiang Suoshu
The water of volume needed for being added in activation of microorganism culture tank；Strain is seeded to the activation of microorganism culture with required inoculum concentration
In tank, by the way that temperature electrode, pH electrode and the best training of dissolved oxygen electrode setting inside the activation of microorganism culture tank is arranged in
The condition of supporting, activation culture.Wherein, temperature electrode, pH electrode and dissolved oxygen electrode output are received by electric automatic control cabinet
Signal, thus each parameter value in real time monitoring and adjusting activation of microorganism culture tank.Wherein, the temperature electrode is for perceiving
Cultivation temperature in activation of microorganism culture tank, pH electrode is used to perceive the pH value of culture medium in activation of microorganism culture tank, molten
Oxygen electrode is used to perceive the oxygen dissolving value of culture medium in activation of microorganism culture tank.
It wherein, further include temperature control system, pH system, aerating system and illumination system, institute in activation of microorganism culture tank
It states temperature control system to be connected with the electric automatic control cabinet, the letter that the electric automatic control cabinet is exported according to the temperature electrode
Number control temperature control system adjusts the temperature in the activation of microorganism culture tank, and the electric automatic control cabinet is according to
The signal of pH electrode output controls the pH value for the culture solution that the pH system is adjusted in the activation of microorganism culture tank, the electricity
Gas automatic control cabinet controls the aerating system according to the signal that the dissolved oxygen electrode exports and adjusts the activation of microorganism culture
The oxygen dissolving value of culture solution in tank.
In a preferred embodiment of the invention, it is additionally provided with temperature control heating pipe in activation of microorganism culture tank and/or changes
Hot-water line, the temperature control heating pipe access the electric automatic control cabinet, and the electric automatic control cabinet is according to the temperature electricity
The output of pole controls unlatching and closing for the temperature control heating pipe.The water inlet end of the heat exchanging water pipe is lived with the microorganism is located at
The heat exchange water pump changed outside culture tank is connected, and the electric automatic control cabinet controls the heat exchange according to the output of the temperature electrode
Water pump unlatches and closes.I.e. temperature control system includes above-mentioned temperature control heating pipe and heat exchanging water pipe.When electric automatic control cabinet is from temperature
When perceiving temperature change in culture tank at degree electrode, heat exchange water pump and/or temperature control heating pipe start automatic work, pass through temperature control
Heating tube and/or heat exchange water pump control temperature in culture tank.If being provided with heat exchanging water pipe, draining is equipped at water outlet
Water after heat exchange is discharged pipe.Wherein, the setting position of temperature control heating pipe and/or heat exchanging water pipe can be set according to the design needs
Meter, as long as not influencing its temperature controlling function.More preferably, it is additionally provided with temperature control heating pipe in activation of microorganism culture tank and changes
Hot-water line.When temperature is too low in tank, temperature control heating pipe, which is started to work, to be heated；When temperature is excessively high, automatic electrical control system is opened
Exchange heat water pump, and cooling water enters heat exchanging water pipe and takes away waste heat.
It further include being trained with the activation of microorganism in a preferred embodiment of the invention, in activation of microorganism culture tank
The connected acid & alkali liquid feed pump of tank is supported, the acid & alkali liquid feed pump accesses the electric automatic control cabinet, the electrical automatic control
Cabinet processed controls unlatching and closing for the acid & alkali liquid feed pump according to the pH electrode.I.e. pH system includes acid & alkali liquid feed pump.
When electric automatic control cabinet perceives pH value variation in culture tank from pH electrode, acid & alkali liquid feed pump automatically begins to work,
To adjust the pH value of culture medium in culture tank.Preferably, feed pump, that is, acid solution feed pump and lye feed pump containing there are two,
It is accessed in culture tank by perfusion tube, when pH is lower in tank, lye feed pump is automatically turned on, and adjustment pH gos up；As pH in tank
When higher, acid solution feed pump is automatically turned on.
In a preferred embodiment of the invention, aerator, the aeration are additionally provided in activation of microorganism culture tank
Device is connected by pipeline with the air pump being located at outside the activation of microorganism culture tank, and the electric automatic control cabinet is according to institute
The output for stating dissolved oxygen electrode controls unlatching and closing for the air pump.I.e. aerating system includes aerator and air pump.When
From when perceiving oxygen dissolving value reduction in culture tank from dissolved oxygen electrode, air switch pump automatically opens electric automatic control cabinet, makes sky
Gas enters pipeline, and aerator is started to work, and further adjusts the oxygen dissolving value of culture medium in culture tank.Wherein, aerator can
Think microporous aeration disc.Microporous aeration disc is connected by aeration tube with air pump.When dissolved oxygen is lower in tank, air pump is opened automatically
It opens, aeration oxygen replenishing；When dissolved oxygen reaches Spawn incubation requirement, air pump is automatically closed, and saves electric energy.
In a preferred embodiment of the invention, the top of the activation of microorganism culture tank is equipped with illumination apparatus, institute
It states electric automatic control cabinet and controls unlatching and closing for the illumination apparatus.In the actual operation process, when illumination condition is inadequate
When, electric automatic control cabinet can be by controlling the illumination item in the illumination apparatus adjusting cultivating system for being located at culture tank top
Part, to reach optimal condition of culture.
In a preferred embodiment of the invention, in order to further realize automation, intake pump Xiang Weisheng can be passed through
Add water in object activation culture tank, wherein the content of water can be controlled by self-timing control system.Preferably, micro- life
The top of object activation culture tank is connected with intake pump, and the intake pump is by the timing controlled that is located in the electric automatic control cabinet
System control.Water-level gauge can be set in activation of microorganism culture tank, so as to more accurately regulate and control water level.Intake pump with it is micro-
Bioactivation culture tank is connected by inlet pipeline.
In a preferred embodiment of the invention, in order to be able to achieve sprinkling, the bottom of activation of microorganism culture tank passes through
Water management high-pressure hydraulic pump is connected with flusher out, and the water management high-pressure hydraulic pump out is controlled by the electric automatic control cabinet.
Culture pot bottom is connected via outlet pipeline with water management high-pressure hydraulic pump out, realizes exact flow rate spray by electric automatic control cabinet
It spills.Preferably, flusher includes microbial inoculum sprinkling outlet pipe and electromagnetic flow valve interconnected, the microbial inoculum sprinkling water outlet
Pipe is connected with the activation of microorganism culture pot bottom, and the sprinkling outlet pipe is high by electromagnetic flow valve and the water management out
Water pump is connected.After the completion of microbial inoculum activation culture, electric automatic control cabinet opens out water management high-pressure hydraulic pump, starts microbial inoculum spray
It spills, fountain height is usually set to 90%, and residue 10% is retained in tank and carries out Semi-continuous cultivation as seed liquor.It wherein, can be with
Including timing means, to realize after the completion of microbial inoculum activation culture, electric automatic control cabinet opens out water management high-pressure hydraulic pump.
In a preferred embodiment of the invention, the activation of microorganism culture tank and electric automatic control cabinet are placed in case
In formula protection cabinets, the top and front of the box protection cabinets are equipped with opening and removable door corresponding with the opening.That is case
Formula protection cabinets top and front are openable, facilitate operation and observation, usually when unattended device automatic running, close box guarantor
Cabinet is protected to protect internal structure.Wherein, activation of microorganism culture tank upper cover is also openable, and operation is facilitated to clean.Of the invention is micro-
Bioactivation culture tank can use all-transparent material.Wherein, box protection cabinets can be placed on the fixed bracket of packaged type.Its
In, energy is provided to microbial bacterial agent culture apparatus for convenience, solar panel can be set on protection cabinets top.
It further include instrumented data display platform in electric automatic control cabinet in a preferred embodiment of the invention.It can
Inflow, temperature, pH value, dissolved oxygen situation and the light conditions in activation of microorganism culture tank to be shown.
Another object of the present invention is to provide a kind of microbial bacterial agent cultural methods, include the following steps:
1) nutriment necessary to growth is added in activation of microorganism culture tank；
2) water of volume needed for being added in Xiang Suoshu activation of microorganism culture tank；
3) strain is seeded in the activation of microorganism culture tank with required inoculum concentration, by being arranged in the microorganism
Optimal culture condition, activation culture is arranged in temperature electrode, pH electrode and dissolved oxygen electrode inside activation culture tank.
More preferably, above-mentioned culture can realize semicontinuous automation, i.e. activation of microorganism culture by above-mentioned apparatus
Tank is controlled by intake pump by inlet pipeline and is intake, and inflow is controlled by electric automatic control cabinet time switch, after the completion of water inlet,
For the first time by manually adding in microbial bacterial agent to culture tank, and Biostimulation agent is suitably supplemented according to water inlet nutritive index, promoted
It is bred into growth, opens simultaneously temperature, aeration, pH and the illumination control system in control cabinet, maintained by automatically controlling
The good growth and breeding environment of microorganism.After the completion of culture, time switch opens out water management high pressure feed pump, and microbial inoculum starts certainly
Dynamic sprinkling, water outlet pump is closed after sprinkling, and tank body intake pump opens water inlet, is carried out the semicontinuous culture of microbial inoculum, is so recycled.
The following are specific embodiments, for the present invention is described in further detail.
Embodiment 1
A kind of microbial bacterial agent culture apparatus is present embodiments provided, as shown in Figure 1, mainly including the fixed branch of packaged type
Frame 3, the box protection cabinets 2 being placed on bracket, activation of microorganism culture tank and electric automatic control cabinet 5 inside protection cabinets 2 are set
Rotary type high pressure sprinkler (microbial inoculum sprinkling outlet pipe) outside protection cabinets, the solar panel 1 on protection cabinets top,
In, solar panel can provide energy for whole device.
Box 2 top of protection cabinets and front are openable, facilitate operation and observation, usually unattended device automatic running
When, box protection cabinets 2 are closed to protect internal structure.
4 upper cover of activation of microorganism culture tank is openable, and operation is facilitated to clean, and side surface upper part is equipped with 4 reserved openings and difference
Intake pump, heat exchange water pump, air pump and acid & alkali liquid feed pump are connected by pipeline.Wherein, intake pump is connect by inlet pipeline 14
Logical, cut-in temperature electrode 9, pH electrode 10 and dissolved oxygen electrode 11 inside culture tank, the output end of electrode access electrical automatic control
Cabinet processed.For optimum temperature needed for keeping activation of microorganism culture, temperature control heating pipe 6 and heat exchanging water pipe are installed inside culture tank,
Temperature control heating pipe 6 accesses electric automatic control cabinet 5 by route, is unlatched and closed by control system therein automatic control；It changes
Hot-water line water inlet end connects heat exchange water pump, and heat exchange pump switch accesses control cabinet, and water outlet is equipped with outlet pipe.PH is adjusted by electrical
Automatic control cabinet controls the opening and closing of acid & alkali liquid feed pump, and acid & alkali liquid feed pump includes acid solution feed pipe 15 and lye charging
Pipe 16 is stored in box protection cabinets by manually preparing acid & alkali liquid.Microporous aeration disc 8 is installed inside culture tank, and passes through pipe
Air pump is connected on road, and air switch pump accesses in electric automatic control cabinet, automatically controls aeration according to dissolved oxygen concentration in tank
It unlatches and closes.Culture tank top is equipped with natural light lamp 7, and switch is accessed in electric automatic control cabinet by route.Culture tank
Bottom is equipped with heat exchanging water pipe's discharge outlet and liquid outlet, and liquid outlet connects out water management high-pressure hydraulic pump by outlet tube, out water management
In high-pressure hydraulic pump switch access electric automatic control cabinet 5, controlled by timing control system.
Switched inside electric automatic control cabinet containing device run timing control system, water inlet switch pump, temperature control heating pipe,
Heat exchange pump switch, acid & alkali liquid feed control pump switch, air switch pump, water management high-pressure hydraulic pump switch, natural light lamp are opened out
It closes, operation data display.
High pressure sprinkler 12 is fixed on base support, by box protection cabinets, and can be rotated 360 °, and water outlet is passed through
Pipe 13 connects out water management high-pressure hydraulic pump, and after the completion of activation culture, water management high-pressure hydraulic pump is opened by control cabinet timing controlled out
Closing is opened, realizes the multi-angle exact flow rate sprinkling of microbial inoculum.
Embodiment 2
The present embodiment is the 1 microbial bacterial agent culture and spray operation carried out in conjunction with the embodiments.Device fixation is placed in
In waters bank to be repaired or water body, culture tank upper cover is opened, result is analyzed according to water quality to be administered and prepares a certain amount of life
Object promotor (nutritional sufficiency does not need then to be added), is added in activation of microorganism culture tank, inside electric automatic control cabinet
Intake pump opening time is set, water inlet volume percentage is set as 80%, starts to intake.After the completion of water inlet, intake pump is automatic
It closes, strain is added into culture tank, inoculum concentration is the 1%-10% of culture tank dischargeable capacity.It is special according to the physiology of different strain
Property, sets temperature, pH, dissolved oxygen and light control condition in control cabinet, carries out temperature, soda acid and molten automatically by control system
Oxygen compensation, maintains growth to breed optimum condition, has concurrently set incubation time, starts activation culture.Activation reaches specified
It activates and completes when the time, automatically turned on according to the time high pressure Pump for giving-out of control cabinet setting, sprinkler starts automatic spraying, sprays
Pump for giving-out is automatically closed after the completion, and entire activation is expanded numerous and spraying operation and completed.
Embodiment 3
The present embodiment is 2 activation of ammonia nitrogen removal microbial inoculum and Semi-continuous cultivation sprinkling carried out in conjunction with the embodiments.By the device
It is placed in waters bank to be repaired or water body, opens culture tank upper cover, first culture is added ammonia nitrogen removal microbial inoculum biology and promotes training
Base is supported, to ensure that the fast activating of microbial inoculum is completed, it includes NaCl 0.3g/L, MgSO that biology, which promotes culture medium,4 0.03g/L、
FeSO4 0.03g/L、(NH4)2SO4 0.5g/L、K2HPO45g/L.It is opened in the good intake pump of electric automatic control cabinet inner setting
Duration, water inlet volume percentage are set as 80%, start to intake.After the completion of water inlet, intake pump is automatically closed, and is thrown into culture tank
Add ammonia nitrogen removal microbial inoculum strain, inoculum concentration is the 10% of culture tank dischargeable capacity.Cultivation temperature is set as 30 DEG C in control cabinet, it is molten
Oxygen control is 2mg/L, artificial to prepare 200g/L Na2CO3Solution and 100g/L (NH4)2SO4, the access of lye feed pump input terminal
Na2CO3In solution, acid solution feed pump input terminal accesses (NH4)2SO4In solution, pH control condition is set as pH=7.8, opens alkali
Liquid feed pump.Primary activation incubation time is set as 48h, starts activation culture.After the completion of activation culture, it is discharged by timing controlled
It controls high-pressure hydraulic pump to open, high pressure sprinkler starts automatic spraying, and fountain height is the 90% of the effective microbial inoculum solution of culture tank, remaining
10% bacterium solution, which is retained, is used as Semi-continuous cultivation seed liquor.According to the good spraying time of Pump for giving-out flow set, after the completion of sprinkling, liquid out
Pump is automatically closed.The time intake pump set according to control cabinet starts to intake, while acid solution feed pump is opened and (NH is added4)2SO4Solution, culture tank inside (NH4)2SO4Two pumps are automatically closed after the completion of final concentration of 0.25g/L, water inlet and charging.This is later half
Continuous cultivation cycle is set as 24 hours, and setting acid solution feed pump is opened in culture 12h and 18h, supplements (NH4)2SO4Solution, supplement
Concentration is 0.4g/L, and acid solution feed pump is automatically closed after the completion of each feed supplement.After the completion of culture, timing controlled high pressure Pump for giving-out is opened
It opens, high pressure sprinkler starts automatic spraying, and fountain height is the 90% of the effective microbial inoculum solution of culture tank, and remaining 10% bacterium solution, which is retained, to be made
For Semi-continuous cultivation seed liquor, so recycle.
Finally, method of the invention is only preferable embodiment, it is not intended to limit the scope of the present invention.It is all
Within the spirit and principles in the present invention, any modification, equivalent replacement, improvement and so on should be included in protection of the invention
Within the scope of.
Claims (10)
1. a kind of microbial bacterial agent culture apparatus, which is characterized in that including activation of microorganism culture tank and electric automatic control cabinet；
The molten of the temperature electrode of test temperature, the pH electrode for testing pH and test dissolved oxygen content is equipped in the activation of microorganism culture tank
Oxygen electrode, the output end of the temperature electrode, pH electrode and dissolved oxygen electrode access the electric automatic control cabinet.
2. a kind of microbial bacterial agent culture apparatus according to claim 1, which is characterized in that the activation of microorganism culture
Temperature control heating pipe and/or heat exchanging water pipe are additionally provided in tank, the temperature control heating pipe accesses the electric automatic control cabinet；It is described to change
The water inlet end of hot-water line is connected with the heat exchange water pump being located at outside the activation of microorganism culture tank, the electric automatic control cabinet root
Unlatching and closing for the heat exchange water pump is controlled according to the output of the temperature electrode.
3. a kind of microbial bacterial agent culture apparatus according to claim 1 or 2, which is characterized in that further include with it is described micro-
The connected acid & alkali liquid feed pump of bioactivation culture tank, the acid & alkali liquid feed pump accesses the electric automatic control cabinet, described
Electric automatic control cabinet controls unlatching and closing for the acid & alkali liquid feed pump according to the pH electrode.
4. a kind of microbial bacterial agent culture apparatus according to claim 1 or 2, which is characterized in that the activation of microorganism
Aerator is additionally provided in culture tank, the aerator passes through pipeline and the air being located at outside the activation of microorganism culture tank
Pump is connected, and the air pump is connected with the electric automatic control cabinet, and the electric automatic control cabinet is according to the dissolved oxygen electrode
Output control the air pump and unlatch and close.
5. a kind of microbial bacterial agent culture apparatus according to claim 1 or 2, which is characterized in that the activation of microorganism
The top of culture tank is equipped with illumination apparatus, and the electric automatic control cabinet controls unlatching and closing for the illumination apparatus.
6. a kind of microbial bacterial agent culture apparatus according to claim 1 or 2, which is characterized in that the activation of microorganism
The top of culture tank is connected with intake pump, and the intake pump is by the timing control system control that is located in the electric automatic control cabinet
System.
7. a kind of microbial bacterial agent culture apparatus according to claim 1 or 2, which is characterized in that the activation of microorganism
The bottom of culture tank is connected by going out water management high-pressure hydraulic pump with flusher, and the water management high-pressure hydraulic pump out is by described electrical
Automatic control cabinet control.
8. a kind of microbial bacterial agent culture apparatus according to claim 7, which is characterized in that the flusher includes phase
The microbial inoculum sprinkling outlet pipe and electromagnetic flow valve to connect, the microbial inoculum sprinkling outlet pipe and the activation of microorganism culture tank
Bottom is connected, and the sprinkling outlet pipe is connected by electromagnetic flow valve with the water management high-pressure hydraulic pump out.
9. a kind of microbial bacterial agent culture apparatus according to claim 1 or 2, which is characterized in that the activation of microorganism
Culture tank and electric automatic control cabinet are placed in box protection cabinets, the top of the box protection cabinets and front be equipped with opening and with
The corresponding removable door that is open.
10. a kind of method of microbial bacterial agent culture, which comprises the steps of:
1) nutriment necessary to growth is added in activation of microorganism culture tank；
2) water of volume needed for being added in Xiang Suoshu activation of microorganism culture tank；
3) strain is seeded in the activation of microorganism culture tank with required inoculum concentration, by being arranged in the activation of microorganism
Optimal culture condition, activation culture is arranged in temperature electrode, pH electrode and dissolved oxygen electrode inside culture tank.
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| CN201811604770.1A CN109576133A (en) | 2018-12-26 | 2018-12-26 | A kind of microbial bacterial agent culture apparatus and cultural method |
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| CN201811604770.1A CN109576133A (en) | 2018-12-26 | 2018-12-26 | A kind of microbial bacterial agent culture apparatus and cultural method |
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| RU2783515C1 (en) * | 2022-07-22 | 2022-11-14 | Федеральное государственное автономное образовательное учреждение высшего образования "Белгородский государственный национальный исследовательский университет" (НИУ "БелГУ") | Device for assessing the intensity of respiration of microbial culture |
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