CN207973746U - Microbial bacterial agent culture apparatus - Google Patents

Microbial bacterial agent culture apparatus Download PDF

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Publication number
CN207973746U
CN207973746U CN201820203985.1U CN201820203985U CN207973746U CN 207973746 U CN207973746 U CN 207973746U CN 201820203985 U CN201820203985 U CN 201820203985U CN 207973746 U CN207973746 U CN 207973746U
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tank
connect
culture
bacterial agent
solenoid valve
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CN201820203985.1U
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Inventor
王冬
宋宏连
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Beijing Kangyuan Oasis Biotechnology Group Co ltd
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KOUGEN RISOU BIOTECH Co Ltd
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Abstract

The utility model discloses a kind of microbial bacterial agent culture apparatus, steam sterilization system can effectively sterilize culture tank and storage tank, and avoiding miscellaneous bacteria from entering influences microbial inoculum quality, ensure that the sterile growth environment of strain;By temperature sensor, pH sensors, oxygen concentration sensor in culture tank, meet temperature, pH value and the oxygen concentration needed for growth, improves strain survival rate and growth rate.In addition, microbial bacterial agent culture apparatus overall structure provided by the utility model is simple, temperature, pH value, the oxygen concentration in culture tank are easily controllable, are not easily susceptible to pollute, improve product quality.

Description

Microbial bacterial agent culture apparatus
Technical field
The utility model is related to microbial inoculum culture field, especially a kind of microbial bacterial agent culture apparatus.
Background technology
Multiple culture tanks can be used in the production process of fermentative microorganism microbial inoculum, multiple culture tanks need to be connected to setting, and It in the fermentation process of fermentative microorganism microbial inoculum, needs to sterilize the inside of tank body, existing sterilization mode is mostly to heat Formula is sterilized, and the consumption energy is larger, and bactericidal effect is poor, and needing to coordinate the agitating device inside culture tank constantly to stir could be complete At, therefore using very inconvenient, and culture tank needs each individual sterilization and disinfection device of self-configuring, therefore make enterprise investment compared with Greatly, it is unfavorable for mitigating cost of investment, while also constrains the improvement of microbial bacteria agent producing process.
Utility model content
In view of this, the purpose of this utility model is to provide a kind of microbial bacterial agent culture apparatus, miscellaneous bacteria is avoided to enter shadow Microbial inoculum quality is rung, ensure that the sterile growth environment of strain.
To achieve the goals above, the utility model provides the following technical solutions:
A kind of microbial bacterial agent culture apparatus, including:
Culture tank, several storage tanks and steam sterilization system;
The steam sterilization system is connect with the culture tank and several storage tanks respectively;The culture tank goes out liquid Mouth is connect with several storage tanks respectively;
Cover is provided at the top of the culture tank, the cover is removably mounted at the top of the culture tank;
It is provided with feeding port, water inlet, the first oxygen port, the first nitrogen mouth and exhaust outlet on the cover, described first Nitrogen mouth is connect by the first solenoid valve with nitrogen gas tank, and first oxygen port is connect by second solenoid valve with oxygen tank;
It is provided with exhaust pipe on the exhaust outlet, check-valves is provided on the exhaust pipe;
The feeding port is connect by strain feed pump with strain case;The water inlet is connect by water pump with water tank;
The culture tank includes outer tank body and inner tank body, shape between the outer wall of the inner tank body and the inner wall of the outer tank body At interlayer cavity, heating device is provided in the interlayer cavity;
Temperature sensor, pH sensors, oxygen concentration sensor are provided in the culture tank;
First solenoid valve, the second solenoid valve, the heating device, the temperature sensor, pH sensings Device, the oxygen concentration sensor, the steam sterilization system, the check-valves are connect with controller respectively.
Preferably, it is provided with the second oxygen port and the second nitrogen mouth at the top of the storage tank, second oxygen port passes through Third solenoid valve is connect with the oxygen tank, and the second nitrogen mouth is connect by the 4th solenoid valve with the nitrogen gas tank;
The third solenoid valve is connect with the controller respectively with the 4th solenoid valve.
Preferably, the steam sterilization system includes shell, and the shell inner top is provided with steam generator, the steaming It is provided with aqua storage tank below vapour generator, steam port is provided at the top of the steam generator;The case top is provided with out Gas port, the gas outlet are connect with the culture tank and several storage tanks respectively;
The culture tank and several one side bottoms of the storage tank are both provided with water outlet;
The steam generator is connect with the controller.
Preferably, the heating device includes the heating electrode being arranged in the interlayer cavity and is arranged in the culture tank The heater being connect outside with the heating electrode.
Preferably, the heating device includes the medium being arranged in the interlayer cavity and is arranged outside the culture tank Heater, the import of the interlayer cavity are pumped by first circulation and are connect with the heater, and the outlet of the interlayer cavity passes through the Two circulating pumps are connect with the heater.
Preferably, ultrasonic generator is provided on the outer tank body outer wall, the ultrasonic generator connects with the controller It connects.
Preferably, the storage tank is made of transparent material, and graduation mark is provided on the storage tank.
Preferably, the water inlet is connect by filter device with the water tank.
A kind of microbial bacterial agent culture apparatus provided by the utility model, steam sterilization system can effectively to culture tank and Storage tank is sterilized, and avoiding miscellaneous bacteria from entering influences microbial inoculum quality, ensure that the sterile growth environment of strain;By in culture tank Interior temperature sensor, pH sensors, oxygen concentration sensor meet temperature, pH value and oxygen concentration needed for growth, carry High strain survival rate and growth rate.In addition, microbial bacterial agent culture apparatus overall structure provided by the utility model is simple, training Temperature, pH value, the oxygen concentration supported in tank are easily controllable, are not easily susceptible to pollute, improve product quality.
Description of the drawings
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only It is some embodiments of the utility model, for those of ordinary skill in the art, in the premise not made the creative labor Under, other drawings may also be obtained based on these drawings.
Fig. 1 is the structural schematic diagram of microbial bacterial agent culture apparatus in the utility model.
In figure:
1, culture tank;2, storage tank;3, steam sterilization system;4, feeding port;5, water inlet;6, the first oxygen port;7, One nitrogen mouth;8, oxygen tank;9, strain case;10, water tank;11, outer tank body;12, inner tank body;13, steam generator;14, water storage Slot;15, nitrogen gas tank.
Specific implementation mode
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work The every other embodiment obtained, shall fall within the protection scope of the present invention.
As shown in Figure 1, a kind of microbial bacterial agent culture apparatus provided by the utility model, including:
Culture tank 1, several storage tanks 2 and steam sterilization system 3;
Steam sterilization system 3 is connect with culture tank 1 and several storage tanks 2 respectively;The liquid outlet of culture tank 1 respectively with it is several Storage tank 2 connects;
1 top of culture tank is provided with cover, and cover is removably mounted on 1 top of culture tank;
Feeding port 4, water inlet 5, the first oxygen port 6, the first nitrogen mouth 7 and exhaust outlet, the first nitrogen are provided on cover Mouth 7 is connect by the first solenoid valve with nitrogen gas tank 15, and the first oxygen port 6 is connect by second solenoid valve with oxygen tank 8;
It is provided with exhaust pipe on exhaust outlet, check-valves is provided on exhaust pipe;
Feeding port 4 is connect by strain feed pump with strain case 9;Water inlet 5 is connect by water pump with water tank 10;
Culture tank 1 includes outer tank body 11 and inner tank body 12, is formed between the outer wall and the inner wall of outer tank body 11 of inner tank body 12 Interlayer cavity is provided with heating device in interlayer cavity;
Temperature sensor, pH sensors, oxygen concentration sensor are provided in tank body;
First solenoid valve, second solenoid valve, heating device, temperature sensor, pH sensors, oxygen concentration sensor, steaming Vapour sterilizing unit 3, check-valves are connect with controller respectively.
In above-mentioned technical proposal, steam sterilization system 3 can effectively sterilize culture tank 1 and storage tank 2, avoid miscellaneous Bacterium, which enters, influences microbial inoculum quality, ensure that the sterile growth environment of strain;Pass through temperature sensor, pH sensings in culture tank 1 Device, oxygen concentration sensor meet temperature, pH value and oxygen concentration needed for growth, improve strain survival rate and growth Rate.In addition, microbial bacterial agent culture apparatus overall structure provided by the utility model is simple, temperature, pH in culture tank 1 Value, oxygen concentration are easily controllable, are not easily susceptible to pollute, improve product quality.
Culture tank 1 is for providing stable condition of culture for thalline, for thalli growth.The setting energy of cover in culture tank 1 It is enough that culture tank 1 is sealed, is cleaned, is safeguarded.
Storage tank 2 is used to store the thalline that culture obtains.
For sterilizing to culture tank 1 and storage tank 2, guarantee strain is grown steam sterilization system 3 in an aseptic environment, It avoids miscellaneous bacteria from entering, influences strain quality.
Feeding port 4, water inlet 5, the first oxygen port 6, the first nitrogen mouth 7 and exhaust outlet, the first nitrogen are provided on cover Mouth 7 is connect by the first solenoid valve with nitrogen gas tank 15, and the first oxygen port 6 is connect by second solenoid valve with oxygen tank 8;Wherein, Feeding port 4 is connect by strain feed pump with strain case 9, to facilitate the addition of strain;Water inlet 5 is convenient to be carried for growth For moisture;First nitrogen mouth 7 is connect by the first solenoid valve with nitrogen gas tank 15, and the first oxygen port 6 passes through second solenoid valve and oxygen Gas tank 8, so as to effectively grow the oxygen for improving suitable concentration for microbial inoculum;The setting of exhaust outlet is equally used for adjusting culture tank Oxygen concentration in 1, the pressure that on the other hand can also be reduced in culture tank 1 are discharged when oxygen concentration is excessively high by exhaust outlet Appropriate gas, the first nitrogen mouth 7 are filled with nitrogen, reduce the oxygen concentration in culture tank 1, ensure the pressure in culture tank 1;Work as oxygen When gas concentration is too low, appropriate gas is discharged by exhaust outlet, the first oxygen port 6 is filled with oxygen, and the oxygen reduced in culture tank 1 is dense Degree ensures the pressure in culture tank 1.
Culture tank 1 includes outer tank body 11 and inner tank body 12, is formed between the outer wall and the inner wall of outer tank body 11 of inner tank body 12 Interlayer cavity is provided with heating device in interlayer cavity;Above-mentioned setting can conveniently and effectively carry out microbial bacterial agent culture apparatus Heating.
Temperature sensor, pH sensors, oxygen concentration sensor are provided in tank body;Above-mentioned setting can monitor effectively, Detect temperature, pH value and the oxygen concentration in tank body.
Controller is by controlling the first solenoid valve, second solenoid valve, heating device, temperature sensor, pH sensors, oxygen Concentration sensor and steam sterilization system 3 provide suitable temperature, pH value and oxygen concentration and anaerobic for the growth of strain Environment, it is ensured that strain is grown in an aseptic environment.
In the present embodiment, 2 top of storage tank is provided with the second oxygen port and the second nitrogen mouth, and the second oxygen port passes through Third solenoid valve is connect with oxygen tank 8, and the second nitrogen mouth is connect by the 4th solenoid valve with nitrogen gas tank 15;Third solenoid valve and Four solenoid valves are connect with controller respectively.Microbial inoculum can be convenient for preserve in above-mentioned setting so that microbial inoculum stay active for long periods.
In the present embodiment, steam sterilization system 3 includes shell, and shell inner top is provided with steam generator 13, is steamed 13 lower section of vapour generator is provided with aqua storage tank 14, and 13 top of steam generator is provided with steam port;Case top is provided with outlet Mouthful, gas outlet is connect with culture tank 1 and several storage tanks 2 respectively;Steam generator 13 is connect with controller;If culture tank 1 and 2 one side bottom of dry storage tank is both provided with water outlet.In above-mentioned setting, can effectively it go out to culture tank 1 and storage tank 2 Water in aqua storage tank 14 can be become high-temperature vapour by bacterium, steam generator 13, and high-temperature vapour is from entering culture tank 1 and several deposit Storage tank 2, achievees the effect that sterilizing.
It should be noted that water outlet is connect with outlet pipe, valve is provided on outlet pipe, valve connects with controller It connects.
In the present embodiment, heating device includes the heating electrode being arranged in interlayer cavity and is arranged outside culture tank 1 The heater being connect with heating electrode.In above-mentioned setting, heater heats electrode by heating, to heat culture tank 1.
In the present embodiment, heating device include the medium being arranged in interlayer cavity and be arranged outside culture tank 1 plus Hot device, the import of interlayer cavity is pumped by first circulation to be connect with heater, and the outlet of interlayer cavity is pumped and heated by second circulation Device connects.In above-mentioned setting, heater is by heat medium, for heating culture tank 1.
In the present embodiment, ultrasonic generation is provided in order to realize that strain is mixed well with zymotic fluid, on tank wall Device, ultrasonic generator are connect with controller.
In the present embodiment, storage tank 2 is made of transparent material, and graduation mark is provided on storage tank 2.
In the present embodiment, in order to avoid miscellaneous bacteria is mixed into, microbial inoculum quality is influenced, water inlet 5 passes through filter device and water Case 10 connects.
The foregoing description of the disclosed embodiments enables professional and technical personnel in the field to realize or using this to institute's public affairs The above description for the embodiment opened enables professional and technical personnel in the field to realize or use the utility model.To these implementations A variety of modifications of example will be apparent to those skilled in the art, and the general principles defined herein can Without departing from the spirit or scope of the present utility model, to realize in other embodiments.Therefore, the utility model will It will not be intended to be limited to the embodiments shown herein, and be to fit to and principles disclosed herein and novel features phase one The widest range caused.

Claims (8)

1. a kind of microbial bacterial agent culture apparatus, which is characterized in that including:
Culture tank, several storage tanks and steam sterilization system;
The steam sterilization system is connect with the culture tank and several storage tanks respectively;The liquid outlet of the culture tank point It is not connect with several storage tanks;
Cover is provided at the top of the culture tank, the cover is removably mounted at the top of the culture tank;
Feeding port, water inlet, the first oxygen port, the first nitrogen mouth and exhaust outlet, first nitrogen are provided on the cover Mouth is connect by the first solenoid valve with nitrogen gas tank, and first oxygen port is connect by second solenoid valve with oxygen tank;
It is provided with exhaust pipe on the exhaust outlet, check-valves is provided on the exhaust pipe;
The feeding port is connect by strain feed pump with strain case;The water inlet is connect by water pump with water tank;
The culture tank includes outer tank body and inner tank body, and folder is formed between the outer wall of the inner tank body and the inner wall of the outer tank body Layer chamber, is provided with heating device in the interlayer cavity;
Temperature sensor, pH sensors, oxygen concentration sensor are provided in the culture tank;
First solenoid valve, the second solenoid valve, the heating device, the temperature sensor, the pH sensors, institute Oxygen concentration sensor, the steam sterilization system, the check-valves is stated to connect with controller respectively.
2. microbial bacterial agent culture apparatus according to claim 1, which is characterized in that be provided with the at the top of the storage tank Two oxygen ports and the second nitrogen mouth, second oxygen port are connect by third solenoid valve with the oxygen tank, second nitrogen Gas port is connect by the 4th solenoid valve with the nitrogen gas tank;
The third solenoid valve is connect with the controller respectively with the 4th solenoid valve.
3. microbial bacterial agent culture apparatus according to claim 1, which is characterized in that the steam sterilization system includes shell Body, the shell inner top are provided with steam generator, aqua storage tank, the steam generation are provided with below the steam generator It is provided with steam port at the top of device;The case top is provided with gas outlet, the gas outlet respectively with the culture tank and several The storage tank connection;
The culture tank and several one side bottoms of the storage tank are both provided with water outlet;
The steam generator is connect with the controller.
4. microbial bacterial agent culture apparatus according to claim 1, which is characterized in that the heating device includes that setting exists Heating electrode in the interlayer cavity and it is arranged outside the culture tank and heater that the heating electrode is connect.
5. microbial bacterial agent culture apparatus according to claim 1, which is characterized in that the heating device includes that setting exists Medium in the interlayer cavity and the heater being arranged outside the culture tank, the import of the interlayer cavity are pumped by first circulation It is connect with the heater, the outlet of the interlayer cavity is pumped by second circulation to be connect with the heater.
6. microbial bacterial agent culture apparatus according to claim 1, which is characterized in that be provided on the outer tank body outer wall Ultrasonic generator, the ultrasonic generator are connect with the controller.
7. microbial bacterial agent culture apparatus according to claim 1, which is characterized in that the storage tank is transparent material system At being provided with graduation mark on the storage tank.
8. microbial bacterial agent culture apparatus according to claim 1, which is characterized in that the water inlet passes through filter device It is connect with the water tank.
CN201820203985.1U 2018-02-05 2018-02-05 Microbial bacterial agent culture apparatus Active CN207973746U (en)

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Application Number Priority Date Filing Date Title
CN201820203985.1U CN207973746U (en) 2018-02-05 2018-02-05 Microbial bacterial agent culture apparatus

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Application Number Priority Date Filing Date Title
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109576133A (en) * 2018-12-26 2019-04-05 湖北省长江水生态研究院有限责任公司 A kind of microbial bacterial agent culture apparatus and cultural method
CN110684664A (en) * 2019-12-02 2020-01-14 邯郸市正德节能环保有限公司 Biological bacterium culture method
CN112266882A (en) * 2020-10-21 2021-01-26 重庆灵水生物科技有限公司 Preparation method of micro-ecological probiotic preparation
CN113830969A (en) * 2021-09-09 2021-12-24 新沂市沂兰绿色材料产业研究院有限公司 Intelligent microbial treatment chemical wastewater equipment

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109576133A (en) * 2018-12-26 2019-04-05 湖北省长江水生态研究院有限责任公司 A kind of microbial bacterial agent culture apparatus and cultural method
CN110684664A (en) * 2019-12-02 2020-01-14 邯郸市正德节能环保有限公司 Biological bacterium culture method
CN112266882A (en) * 2020-10-21 2021-01-26 重庆灵水生物科技有限公司 Preparation method of micro-ecological probiotic preparation
CN113830969A (en) * 2021-09-09 2021-12-24 新沂市沂兰绿色材料产业研究院有限公司 Intelligent microbial treatment chemical wastewater equipment

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Address after: Room 618, 6th floor, Building 26 Anningzhuang Road, Haidian District, Beijing, 100000

Patentee after: Beijing Kangyuan Oasis Biotechnology Group Co.,Ltd.

Address before: Room 318, Office Building 3, No. 4 Anningzhuang Road, Qinghe, Haidian District, Beijing, 100000

Patentee before: KOUGEN RISOU BIOTECH (BEIJING) CO.,LTD.