CN209702736U - A kind of liquid bacterial culture device - Google Patents
A kind of liquid bacterial culture device Download PDFInfo
- Publication number
- CN209702736U CN209702736U CN201821288238.9U CN201821288238U CN209702736U CN 209702736 U CN209702736 U CN 209702736U CN 201821288238 U CN201821288238 U CN 201821288238U CN 209702736 U CN209702736 U CN 209702736U
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- tank body
- air
- pipeline
- pump
- bacterial culture
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Abstract
The utility model discloses a kind of liquid bacterial culture devices, including tank body, the center position of the top of the tank is provided with magnetic stirring apparatus, center position above the magnetic stirring apparatus is provided with stirring rod, spiral collet is provided on the side wall of the tank body, the right end of the tank body is provided with support frame, air pump is installed above support frame as described above, the lower section of the air pump is connected with air accumulator by pipeline, the lower section of the air accumulator is connected with vacuum pump by pipeline, this liquid bacterial culture device is internally provided with the stirring rod that can be ventilated, sufficient oxygen can be provided for strain, and wavy stirring flabellum is provided on stirring rod, strain is carried out using the effect stirred to liquid and is stirred, strain is injured small, the present apparatus is also provided with air accumulator, the gas being passed through is by the quartz burner sterilization in air accumulator, and Source gas is the air in tank, avoids the risk for increasing infection with contacting external air.
Description
Technical field
The utility model belongs to technical field of bioengineering, and in particular to a kind of liquid bacterial culture device.
Background technique
Liquid spawn is to pass through the liquid form of liquid fermentation technology production in biological fermentation tank with fluid nutrient medium
Edible fungus species, China's Edible Fungi be still traditional craftization, workshop-based, huge numbers of families peasant production, compare
For strain cultivation, production cost, growth time are all higher than strain cultivation, but still some are not or not strain cultivation
Foot.
It is still mechanical stirring that current liquid bacterial culture device, which has some, since the shearing force that stirring flabellum generates easily is hurt
Mycelium pellet makes bacterium ball become monokaryon shape, can not fully absorb nutrition and become larger, although some using gases stir, but not
Energy long term storage is easy that strain is made to produce knot, and is easy to grow mycoderma, and the gas of Gas Stirring is collected from the external world, is not passed through
Bacteria removing increases infection rate, etc. more some problems and us is needed to solve.
Utility model content
The purpose of this utility model is to provide a kind of liquid bacterial culture devices, mentioned above in the background art to solve
It is still mechanical stirring that current liquid bacterial culture device, which has some, since the shearing force that stirring flabellum generates easily hurts mycelium pellet,
So that bacterium ball is become monokaryon shape, nutrition can not be fully absorbed and become larger, although some using gases stir, but cannot store up for a long time
It deposits, is easy that strain is made to produce knot, and be easy to grow mycoderma, and the gas of Gas Stirring is collected from the external world, does not pass through bacteria removing,
The problem of increasing infection rate.
To achieve the above object, the utility model provides the following technical solutions: a kind of liquid bacterial culture device, including tank
Body, the left end of the top of the tank offer feed inlet, and feed cap, the right side of the feed inlet are provided with above the feed inlet
End is provided with exhaust pipe, exhaust cap is provided with above the exhaust pipe, the right end of the top of the tank is provided with pressure gauge, institute
The right end for stating pressure gauge offers gas vent, and the right end of the gas vent is provided with thermometer table, the center of the top of the tank
It is provided with magnetic stirring apparatus at position, the center position above the magnetic stirring apparatus is provided with stirring rod, under the tank body
The right side opening of side is equipped with discharge gate, is provided with discharge lid on the right side of the discharge gate, heating group is provided with below the tank body
Part is provided with fixed plate below the heating component, and the fixed plate is fixedly connected by bolt with the bottom of tank body, described
The bottom of tank body is provided with support leg, and spiral collet, the water inlet on the left of the spiral collet are provided on the side wall of the tank body
It is provided with condenser at mouthful, compressor is connected with below the condenser, is provided with steam generation below the compressor
Case, the left end above the steam generating box offer water inlet, are provided with heating tube on the left of the steam generation chamber interior wall,
The right end of the steam generating box is connected with centrifugal pump by pipeline, and the centrifugal pump passes through the water outlet of pipeline and spiral collet
Connection, the right end of the tank body is provided with support frame, is equipped with air pump above support frame as described above, the air pump by pipeline with
The top of stirring rod is rotatablely connected, and the lower section of the air pump is connected with air accumulator by pipeline, and the air accumulator is solid by clip
Determine on the support frame, the lower section of the air accumulator is connected with vacuum pump by pipeline, and the vacuum pump is fixed on the support frame, institute
The left side for stating vacuum pump is connected by pipeline and the gas vent of top of the tank, and the right end of support frame as described above is provided with console.
Preferably, the stirring rod is through-hole structure, and the stirring rod is by guide rod, wave sheets, air-guide rod, escape pipe group
At the left and right ends of the escape pipe are provided with mutual corresponding venthole, are provided with axis on the inner wall of the bottom of the tank body
It holds, the bottom of the air-guide rod and bearing are rotatablely connected.
Preferably, the magnetic stirring apparatus is internally provided with two or so mutual corresponding magnetic force stones, and the magnetic force stone is remote
One end from guide rod is provided with insulation sheet rubber, and the electro-insulating rubber on piece is fixedly connected by telescopic rod, and the telescopic rod is fixed
In the inner wall of magnetic stirring apparatus.
Preferably, the spiral collet is internally provided with spoiler, and the bottom of the spoiler is provided with aperture.
Preferably, the air accumulator is internally provided with three quartz burners, and the quartz burner is equidistantly vertical
Arrangement, the heating tube, heating component, telescopic rod, guide rod, quartz burner, compressor, centrifugal pump, air pump and vacuum pump are equal
It is electrically connected with the switch on console, the switch on the console is connect by power outlet with external power supply
Preferably, shut-off valve is installed on the discharge gate of the tank body, is installed at the pipeline that the air pump is connect with guide rod
There is shut-off valve, shut-off valve, the vacuum pump and gas storage are installed at the pipeline that the vacuum pump is connect with the gas vent on tank body
Shut-off valve is installed at tank connected pipeline, shut-off valve is installed at the air pump and the tank connected pipeline of gas storage.
Compared with prior art, the utility model has the beneficial effects that be internally provided with can be with for the liquid bacterial culture device
The stirring rod of ventilation can provide sufficient oxygen for strain, and wavy stirring flabellum is provided on stirring rod, using pair
The effect that liquid is stirred is carried out strain and is stirred, small to strain injury, and the present apparatus is also provided with air accumulator, and the gas being passed through passes through
Quartz burner sterilization in air accumulator, and source gas is the air in tank, avoid with contacting external air and increase infection
Risk, present apparatus screw clamp, which is put on, is provided with spoiler, can extend cooling water retention time, reaches the mesh being fully cooled
's.
Detailed description of the invention
FIG. 1 is a schematic structural view of the utility model；
Fig. 2 is the main view of the utility model；
Fig. 3 is the magnetic stirring apparatus top view of the utility model；
Fig. 4 is the stirring rod main view of the utility model
Fig. 5 is the partial enlarged view in Fig. 4 at A.
In figure: 1- tank body, 2- spiral collet, 3- condenser, 4- compressor, 5- water inlet, 6- steam generating box, 7- heating
Pipe, 8- centrifugal pump, 9- support leg, 10- fixed plate, 11- heating component, 12- bearing, 13- stirring rod, 14- shut-off valve, 15- row
Material mouth, 16- discharge lid, 17- vacuum pump, 18- air accumulator, 19- console, 20- quartz burner, 21- clip, 22- air pump,
23- thermometer table, 24- gas vent, 25- pressure gauge, 26- magnetic stirring apparatus, 27- exhaust cap, 28- exhaust pipe, 29- feed cap,
30- feed inlet, 31- support frame, 32- telescopic rod, 33- insulation sheet rubber, 34- magnetic force stone, 35- wave sheets, 36- air-guide rod, 37-
Escape pipe, 38- guide rod, 39- spoiler.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
Fig. 1-5 is please referred to, the utility model provides a kind of technical solution: a kind of liquid bacterial culture device, including tank body 1,
The left end at 1 top of tank body offers feed inlet 30, and the top of feed inlet 30 is provided with feed cap 29, the right end setting of feed inlet 30
There is exhaust pipe 27, the top of exhaust pipe 27 is provided with exhaust cap 28, and the right end at 1 top of tank body is provided with pressure gauge 25, pressure gauge
25 right end offers gas vent 24, and the right end of gas vent 24 is provided with thermometer table 23, and the center position of top of the tank is set
It is equipped with magnetic stirring apparatus 26, the center position of 26 top of magnetic stirring apparatus is provided with stirring rod 13, the right side opening of 1 lower section of tank body
Equipped with discharge gate 15, the right side of discharge gate 15 is provided with discharge lid 16, and the lower section of tank body 1 is provided with heating component 11, heating group
The lower section of part 11 is provided with fixed plate 10, and fixed plate 10 is fixedly connected by bolt with the bottom of tank body 1, and the bottom of tank body 1 is set
It is equipped with support leg 9, spiral collet 2 is provided on the side wall of tank body 1, the water inlet in 2 left side of spiral collet is provided with condenser
3, the lower section of condenser 3 is connected with compressor 4, and the lower section of compressor 4 is provided with steam generating box 6,6 top of steam generating box
Left end offers water inlet 5, and heating tube 7 is provided on the left of 6 inner wall of steam generating box, and the right end of steam generating box 6 passes through pipe
Road is connected with centrifugal pump 8, and centrifugal pump 8 is connect by pipeline with the water outlet of spiral collet 2, and the right end of tank body 1 is provided with support
Frame 31, the top of support frame 31 are equipped with air pump 22, and air pump 22 is rotatablely connected by the top of pipeline and stirring rod 13, air pump 22
Lower section air accumulator 18 is connected with by pipeline, air accumulator 18 is fixed on support frame 31 by clip 21, under air accumulator 18
Side is connected with vacuum pump 17 by pipeline, and vacuum pump 17 is fixed on support frame 31, and the left side of vacuum pump 17 passes through pipeline and tank
The gas vent 24 at 1 top of body connects, and the right end of support frame 31 is provided with console 19.
In the present embodiment, it is preferred that stirring rod 13 is through-hole structure, and stirring rod 13 is by guide rod 38, wave sheets 35, air-guide rod
36, escape pipe 37 forms, and the left and right ends of escape pipe 37 are provided with mutual corresponding venthole, on the inner wall of the bottom of tank body 1
It is provided with bearing 12, the bottom of air-guide rod 36 and bearing 12 are rotatablely connected, and wave sheets 35 are wavy shaped configuration, when 13 turns of stirring rod
When dynamic, wave sheets 35 also be can rotate, and be stirred to internal liquid, and generation is stirred, and wave sheets 13 are close to stirring rod 13, volume
It is small, strain will not be caused to damage, and when tank body 1 stores liquid spawn, wave sheets 35 can be made to rotate, flow liquid,
It avoids the production knot of strain, is hollow structure inside air-guide rod 36, is conveyed in air to air-guide rod 36 by air pump 22, then from outlet
Venthole discharge in pipe 37, provides oxygen for strain, with the rotation of stirring rod 13, keeps air delivery each into liquid
Place, the presence of bearing 12 avoid stirring rod 13 and shake during rotation, keep the stability of work.
In the present embodiment, it is preferred that magnetic stirring apparatus 26 is internally provided with two or so mutual corresponding magnetic force stones 34, magnetic
Power stone 34 is provided with insulation sheet rubber 33 far from one end of guide rod 38, is fixedly connected on insulation sheet rubber 33 by telescopic rod 32, is stretched
Contracting bar 32 is fixed on the inner wall of magnetic stirring apparatus 26, when guide rod 38 is powered, since guide rod 38 is located at the centre of magnetic force stone 34,
According to Coulomb's law, so that under conditions of energization, since the effect in magnetic field drives simultaneously so that guide rod 38 ceaselessly rotates
Stirring rod 13 rotates, and internal telescopic rod 32 can control the distance between magnetic force stone 34 and guide rod 38, control the revolving speed of rotation,
There are lead screw, feed screw nut, feed screw nut and 32 cage connection of telescopic rod inside telescopic rod 32, when feed screw nut, which rotates, to be pushed,
Telescopic rod also can be moved and then, be achieved the purpose that flexible.
In the present embodiment, it is preferred that spiral collet 2 is internally provided with spoiler 39, and the bottom of spoiler 39 is provided with
Aperture, spoiler 39 can make cooling water when spiral collet 2 flows, generate a kind of effect of barrier, can extend cooling water
With the time of contact of 1 outer wall of tank body so that cooling it is more abundant.
In the present embodiment, it is preferred that air accumulator 18 is internally provided with three quartz burners 20, quartz burner 20 etc.
Spacing is vertically arranged, heating tube 7, heating component 11, telescopic rod 32, guide rod 38, quartz burner 20, compressor 4, centrifugal pump 8,
Air pump 22 and vacuum pump 17 are electrically connected with the switch on console 19, and the switch on console 19 is by power outlet and outside
Power supply connection is connect, when quartz burner 20 is irradiated to microorganism using ultraviolet light, the transmitting and accumulation of energy occurs, accumulates result
The inactivation of microorganism is caused, to achieve the purpose that disinfection, quartz burner 20 can be killed to being irradiated inside air accumulator 18
Bacterium, so that the air in logical feed liquor body provides the nutrient environment of a safety without bacterium infection for culture solution.
In the present embodiment, it is preferred that shut-off valve 14 is installed, air pump 22 is connect with guide rod 36 on the discharge gate 15 of tank body 1
Pipeline at shut-off valve 14 is installed, shut-off valve 14 is installed at the pipeline that vacuum pump 17 is connect with the gas vent 24 on tank body 1,
Shut-off valve 14 is installed at the pipeline that vacuum pump 17 is connect with air accumulator 18, is installed at the pipeline that air pump 22 is connect with air accumulator 18
There is shut-off valve 14, the shut-off valve 14 on discharge gate 15 facilitates the flow for adjusting discharge, the pipeline that air pump 22 is connect with guide rod 36
14 one side of shut-off valve may be implemented to open and close, cutting at the pipeline that vacuum pump 17 is connect with the gas vent 24 on tank body 1
Only valve 14 may be implemented to open and close, and the shut-off valve 14 at the pipeline that vacuum pump 17 is connect with air accumulator 18 may be implemented to open
Shut-off valve 14 at the pipeline connecting with closing, air pump 22 with air accumulator 18 may be implemented to open and close.
Working principle: when the utility model is used, user passes through console 19 first and opens opening for hot heating component 11
It closes, to high-temperature sterilization is carried out inside tank body 1, the switch of vacuum pump 17 is then opened by console 19, internal air is taken out
Into air accumulator 18, and the switch of quartz burner 20 is opened, after air disinfection, portion of air is put by air pump 22
In tank body 1, after cooling, feed cap 29 is then opened, strain and culture solution is passed through by feed inlet 30 inside of tank body 1, connect
Open magnetic stirring apparatus 26 switch so that stirring rod 13, which is opened, to be made to rotate, wave sheets 35 also be can rotate, to internal bacterium solution carry out
Stirring, generation is stirred, so that strain comes into full contact with oxygen, opens simultaneously the switch of air pump 22, is made in air accumulator 18 by purple
The air that outside line fluorescent tube 20 is sterilized provides suitable oxygen from the venthole discharge in escape pipe 37 for strain, due to fermentation
Temperature is probably in 25 to 28 degree, since the rotation of stirring rod 13 can generate heat, temperature is observed by thermometer table 23, in order to tie up
Temperature is held, such as needs to cool down, water can be passed through in steam generating box 6, and open the switch of heating tube 7, hot steam is made to pass through pressure
Contracting machine 4 becomes cooling water into condenser pipe 3, and flows into screw clamp layer 2, since spiral interlayer 2 is internally provided with spoiler 39, resistance
Flowing plate 39 can make cooling water when spiral collet 2 flows, and generate a kind of effect of barrier, can extend cooling water and tank body 1
The time of contact of outer wall, so that cooling is more abundant, then cooling water flows out spiral interlayer 2, by the effect of centrifugal pump 8 again into
Enter steam generating box, forms a kind of water circulation, it is energy saving, in fermentation process, by observing pressure gauge 25, in order to maintain pressure
Balance, can use true air pump 17 and air is taken away, maintains the air pressure of fermentation, after having fermented, can open exhaust cap 28, connect
Air, and liquid spawn is taken out by discharge gate 15, entire workflow terminates.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired
Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.
Claims (6)
1. a kind of liquid bacterial culture device, including tank body (1), it is characterised in that: the left end at the top of the tank body (1) offer into
Material mouth (30) is provided with feed cap (29) above the feed inlet (30), and the right end of the feed inlet (30) is provided with exhaust
It manages (27), is provided with exhaust cap (28) above the exhaust pipe (27), the right end at the top of the tank body (1) is provided with pressure gauge
(25), the right end of the pressure gauge (25) offers gas vent (24), and the right end of the gas vent (24) is provided with thermometer table
(23), the center position of the top of the tank is provided with magnetic stirring apparatus (26), above the magnetic stirring apparatus (26) in
It is provided at the heart position stirring rod (13), the right side opening below the tank body (1) is equipped with discharge gate (15), the discharge gate (15)
Right side be provided with discharge lid (16), be provided with heating component (11) below the tank body (1), the heating component (11)
Lower section is provided with fixed plate (10), and the fixed plate (10) is fixedly connected by bolt with the bottom of tank body (1), the tank body
(1) bottom is provided with support leg (9), is provided with spiral collet (2) on the side wall of the tank body (1), the spiral collet (2)
The water inlet in left side is provided with condenser (3), is connected with compressor (4), the compressor below the condenser (3)
(4) it is provided with below steam generating box (6), the left end above the steam generating box (6) offers water inlet (5), described
It is provided with heating tube (7) on the left of steam generating box (6) inner wall, the right end of the steam generating box (6) is connected with by pipeline
Centrifugal pump (8), the centrifugal pump (8) are connect by pipeline with the water outlet of spiral collet (2), and the right end of the tank body (1) is set
It is equipped with support frame (31), is equipped with above support frame as described above (31) air pump (22), the air pump (22) passes through pipeline and stirring
The top of bar (13) is rotatablely connected, and the lower section of the air pump (22) is connected with air accumulator (18), the air accumulator by pipeline
(18) it is fixed on support frame (31) by clip (21), the lower section of the air accumulator (18) is connected with vacuum pump by pipeline
(17), the vacuum pump (17) is fixed on support frame (31), and the left side of the vacuum pump (17) is pushed up by pipeline and tank body (1)
The gas vent (24) in portion connects, and the right end of support frame as described above (31) is provided with console (19).
2. a kind of liquid bacterial culture device according to claim 1, it is characterised in that: the stirring rod (13) is through-hole knot
Structure, the stirring rod (13) are made of guide rod (38), wave sheets (35), air-guide rod (36), escape pipe (37), the escape pipe
(37) left and right ends are provided with mutual corresponding venthole, are provided with bearing (12) on the inner wall of the bottom of the tank body (1),
The bottom and bearing (12) of the air-guide rod (36) are rotatablely connected.
3. a kind of liquid bacterial culture device according to claim 1, it is characterised in that: the magnetic stirring apparatus (26) is internal
There are two the mutual corresponding magnetic force stone (34) in left and right, the magnetic force stones (34), and the one end of guide rod (38) is provided with insulation for setting
Sheet rubber (33) is fixedly connected by telescopic rod (32) on the insulation sheet rubber (33), and the telescopic rod (32) is fixed on magnetic force
On the inner wall of blender (26).
4. a kind of liquid bacterial culture device according to claim 1, it is characterised in that: the inside of the spiral collet (2)
It is provided with spoiler (39), the bottom of the spoiler (39) is provided with aperture.
5. a kind of liquid bacterial culture device according to claim 1, it is characterised in that: the inside of the air accumulator (18) is set
It sets there are three quartz burner (20), the quartz burner (20) is equidistantly vertically arranged, the heating tube (7), heating group
Part (11), telescopic rod (32), guide rod (38), quartz burner (20), compressor (4), centrifugal pump (8), air pump (22) and vacuum
(17) are pumped to be electrically connected with the switch on console (19), the switch on the console (19) by power outlet with it is external
Power supply connection.
6. a kind of liquid bacterial culture device according to claim 1, it is characterised in that: on the discharge gate (15) of tank body (1)
It is equipped with shut-off valve (14), is equipped with shut-off valve (14) at the pipeline that the air pump (22) connect with air-guide rod (36), it is described true
Is equipped with shut-off valve (14) at sky pump (17) pipeline for being connect with the gas vent (24) on tank body (1), the vacuum pump (17) and
It is equipped with shut-off valve (14) at the pipeline of air accumulator (18) connection, pacifies at the pipeline that the air pump (22) connect with air accumulator (18)
Equipped with shut-off valve (14).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821288238.9U CN209702736U (en) | 2018-08-10 | 2018-08-10 | A kind of liquid bacterial culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821288238.9U CN209702736U (en) | 2018-08-10 | 2018-08-10 | A kind of liquid bacterial culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209702736U true CN209702736U (en) | 2019-11-29 |
Family
ID=68634872
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821288238.9U Expired - Fee Related CN209702736U (en) | 2018-08-10 | 2018-08-10 | A kind of liquid bacterial culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209702736U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112538429A (en) * | 2020-12-10 | 2021-03-23 | 郭世建 | Cell culture case with control carbon dioxide concentration |
| CN113229041A (en) * | 2021-06-16 | 2021-08-10 | 绵阳菌源博文菌业有限公司 | Domestic fungus liquid fermentation jar convenient to sterilization |
| CN114948429A (en) * | 2022-06-01 | 2022-08-30 | 暨南大学 | Parent-hydrophobic fiber combined processing equipment and processing method |
-
2018
- 2018-08-10 CN CN201821288238.9U patent/CN209702736U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112538429A (en) * | 2020-12-10 | 2021-03-23 | 郭世建 | Cell culture case with control carbon dioxide concentration |
| CN113229041A (en) * | 2021-06-16 | 2021-08-10 | 绵阳菌源博文菌业有限公司 | Domestic fungus liquid fermentation jar convenient to sterilization |
| CN113229041B (en) * | 2021-06-16 | 2023-12-01 | 绵阳菌源博文菌业有限公司 | Edible fungus liquid fermentation tank convenient to sterilize |
| CN114948429A (en) * | 2022-06-01 | 2022-08-30 | 暨南大学 | Parent-hydrophobic fiber combined processing equipment and processing method |
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Granted publication date: 20191129 Termination date: 20200810 |