CN218596393U - Liquid culture shake flask for aerobic microorganisms - Google Patents

Abstract

The utility model discloses an aerobic microorganism liquid culture shakes bottle, including bottle, bottle lid and cassette, the bottle has the bottleneck, the bottle lid closes the bottleneck, the cassette is located the bottleneck with between the bottle lid, the bottle lid includes this somatic part and protruding locating this somatic part is kept away from the portion that keeps out wind of bottle one side, this somatic part is equipped with 4 at least through-holes, the portion that keeps out wind includes that a plurality of are located adjacent two the piece that keeps out wind between the through-hole. The aerobic microorganism liquid culture shake flask can continuously ventilate in the culture process, so that the shake flask keeps high oxygen concentration and low metabolic waste gas concentration.

Description

Liquid culture shake flask for aerobic microorganisms

Technical Field

The utility model relates to a fermentation technology field, more specifically relates to an aerobic microorganism liquid culture shakes bottle.

Background

In the fermentation research and development experiment process and the fermentation production seed preparation process, a small amount of aerobic microorganisms such as bacteria or cells and the like are usually required to be cultured by using a shake flask. During the cultivation process, a conical flask is generally adopted as a flask body of the shake flask, a bottle mouth is wrapped by a plurality of layers of gauze, a film with a filter element, filter paper and other wrapping materials which can filter dust and infectious microbes but have poor air permeability, the shake flask is fixed on a shaking table fixture, and the shake flask continuously performs horizontal circular motion during the cultivation process, so that bacteria liquid continuously moves in the shake flask without a special ventilation structure. In the process, although the air in the bottle generates vortex along with the fluctuation of the liquid culture medium so as to exchange with the gas outside the bottle, when the circular motion speed is low, the bacterial liquid surface hardly generates fluctuation and does not generate gas vortex, and when the bacterial liquid amount is less, the fluctuation of the bacterial liquid is not enough to drive the gas vortex in the bottle with enough strength so as to realize the purpose of exchanging the gas inside and outside the bottle. The low ventilation efficiency can result in the low oxygen content in the bottle and the high concentration of harmful gases (such as carbon dioxide) generated by metabolism in the bottle, thereby causing negative effects on the growth of aerobic microorganisms and finally influencing the culture result.

At present, aiming at the phenomena, a baffle or a fixing piece with other shapes is commonly added in a shake flask bottle, so that the bacterial liquid is severely fluctuated or disturbed in the moving process, and the gas vortex in the flask is enhanced. However, the method of adding the fixing member in the flask inevitably causes the bacterial liquid to be influenced by the shearing force brought by the fixing member, which is contrary to the culture environment of the mature triangular flask with smooth inner wall and no shearing force. The shear force can cause mechanical damage to the bacteria or cells, and the culture result is influenced. In addition, the bottle body is provided with holes or a channel is additionally arranged, and air suction is combined with external equipment, so that the purpose of high-efficiency air exchange can be achieved, but the shake flask is inevitably provided with a plurality of non-detachable structures, dead angles which are difficult to clean exist, and great inconvenience is brought to the shake flask cleaning process.

SUMMERY OF THE UTILITY MODEL

In order to overcome the defects of the prior art, the utility model aims at providing an aerobic microorganism liquid culture shake flask, which can continuously ventilate in the culture process, so that the shake flask keeps high oxygen concentration and low metabolic waste gas concentration.

In order to realize the above-mentioned purpose, the utility model discloses an aerobic microorganism liquid culture shakes bottle, including bottle, bottle lid and cassette, the bottle has the bottleneck, the bottle lid closes the bottleneck, the cassette is located the bottleneck with between the bottle lid, the bottle lid includes this somatic part and protruding locating this somatic part is kept away from the wind-break portion of bottle one side, this somatic part is equipped with 4 at least through-holes, wind-break portion includes a plurality of adjacent two of being located wind-break piece between the through-hole.

Compared with the prior art, when the shake flask is used, because the body part is provided with 4 through holes, the wind-shielding sheet is arranged between every two adjacent through holes, when the shake flask is fixed on the shaking table for aerobic microorganism liquid culture, at least 1 part of space is windward in the positive direction, or at least two parts of space are windward in the oblique direction, and at least 1 part of space is leeward in the oblique direction, or at least two parts of space are leeward in the oblique direction, so that the through holes below the windward space can be filled with air during the horizontal movement of the shake flask, the air in the shake flask is slightly increased, the air in the shake flask can flow out through the through holes below the leeward space, the air outside the shake flask and the air inside the shake flask can continuously and efficiently circulate and exchange, the shake flask can keep high oxygen concentration and low metabolic waste gas concentration, and the influence of shearing force can not be brought.

Preferably, the bottle body is a triangular bottle.

Preferably, the bottle body is provided with a capacity scale mark line.

Preferably, the bottle body and the bottle cap are in threaded fit or snap fit.

Preferably, the bottle body comprises a bottle neck and a bottle body which are integrally formed, and the inner diameter of the bottle neck is equal.

Preferably, the bottle cap is made of plastic or silica gel.

Preferably, the body portion and the wind blocking portion are integrally formed.

Preferably, the body portion is provided with 4 through holes, the wind blocking portion includes 4 wind blocking sheets, and the 4 wind blocking sheets are perpendicular to each other.

Preferably, 4 of the windshield sheets are integrally formed.

Drawings

FIG. 1 is a schematic view of the structure of the liquid culture shake flask for aerobic microorganisms of the present invention.

FIG. 2 is a top view of the liquid culture shake flask for aerobic microorganisms shown in FIG. 1.

FIG. 3 is a side view of the flask of the liquid culture shake flask for aerobic microorganisms shown in FIG. 1.

FIG. 4 is a top view of the flask of the aerobic microbial liquid culture shake flask shown in FIG. 1.

FIG. 5 is a side view of the bottle cap of the aerobic microbial liquid culture shake flask shown in FIG. 1.

FIG. 6 is a top view of the bottle cap in the liquid culture shake flask for aerobic microorganisms shown in FIG. 1.

FIG. 7 is a bottom view of the bottle cap of the aerobic microbial liquid culture shake flask shown in FIG. 1.

Description of the symbols:

the bottle body 10, the bottle opening 11, the bottle neck 13, the bottle body 15, the bottle cap 30, the body part 31, the through hole 311, the wind shielding part 33, the wind shielding sheet 331 and the filter sheet 50.

Detailed Description

In order to explain technical contents, structural features, and objects and effects of the present invention in detail, the following description is given in conjunction with the embodiments and the accompanying drawings.

In the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly, e.g., as being fixedly connected, detachably connected, or integrated; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those of ordinary skill in the art.

Referring to fig. 1-2, the present invention provides an aerobic microbial liquid culture shake flask, including a bottle body 10, a bottle cap 30 and a filter 50, wherein the bottle body 10 has a mouth 11 (as shown in fig. 3), the bottle cap 30 covers the mouth 11, the filter 50 is located between the mouth 11 and the bottle cap 30, the bottle cap 30 includes a body 31 and a wind-shielding portion 33 protruding from one side of the body 31 far away from the bottle body 10, the body 31 has at least 4 through holes 311, and the wind-shielding portion 33 includes a plurality of wind-shielding sheets 331 located between two adjacent through holes 311.

In the above technical solution, when the shake flask is fixed on a shaking table for aerobic microorganism liquid culture, because the body 31 is provided with 4 through holes 311, and a wind-blocking plate 331 is provided between two adjacent through holes 311, at least 1 part of the space is facing the wind in the forward direction, or at least two parts of the space are facing the wind in the oblique direction, and at least 1 part of the space is leeward, or at least two parts of the space are leeward in the oblique direction, so that during the horizontal movement of the shake flask, the through holes 311 below the windward space are filled with air, and the air pressure in the shake flask is slightly increased, so that the air in the shake flask flows out from the through holes 311 below the leeward space, and the air outside the shake flask and the air inside the shake flask are continuously and efficiently circularly exchanged, so that the shake flask maintains high oxygen concentration and low metabolic waste gas concentration, and the influence of shearing force is not brought.

It should be noted that the filter 50 is capable of passing air and trapping dust and bacteria in the air. Preferably, the filter 50 is made of a porous and air-permeable material, and it should be understood that the diameter of the filter 50 is identical to the inner diameter of the cap 30.

Referring to fig. 3-4, in the present embodiment, the bottle body 10 is a triangular bottle, but is not limited to the shape. In a preferred embodiment, the bottle body 10 is provided with a volume scale mark line for easy operation, and the scale mark lines of various specifications can be set according to the volume of the bottle body 10 in actual production. Specifically, the bottle body 10 includes a neck 13 and a body 15, which are integrally formed, and the neck 13 preferably has an inner diameter equal to that of the bottle body 10, so that the bottle body 10 can be tightly attached to the bottle cap 30.

Referring to fig. 1, the bottle body 10 and the bottle cap 30 are in a threaded or snap fit. In a preferred embodiment, the bottle opening 11 of the bottle body 10 is provided with a thread structure on the outer side, the bottle cap 30 is provided with a thread structure on the inner side, and the bottle cap 30 is in threaded connection with the bottle body 10, so that the bottle opening is smooth and gentle.

Referring to fig. 5-7, the bottle cap 30 is made of plastic or silicone. Preferably made of plastic or silica gel with certain elasticity and high temperature sterilization resistance. The bottle cap 30 includes a main body 31 and a wind shielding portion 33, the main body 31 and the wind shielding portion 33 may be formed non-integrally or integrally, and in a preferred embodiment, the main body 31 and the wind shielding portion 33 are formed integrally, which not only increases stability but also prolongs service life.

Referring to fig. 5-7, in the embodiment, the main body 31 has 4 through holes 311, the wind shielding portion 33 includes 4 wind shielding sheets 331, and the 4 wind shielding sheets 331 are perpendicular to each other, but may not be perpendicular. Preferably, the 4 wind-shielding sheets 331 are integrally formed, when in use, the 4 through holes 311 are separated by the 4 wind-shielding sheets 331 in 4 intervals, when the shake flask is fixed on a shaking table for aerobic microorganism liquid culture, at least 1 part of space is windward in a forward direction, or at least two parts of space are windward in an oblique direction, and at least 1 part of space is leeward, or at least two parts of space are leeward in an oblique direction, so that during the horizontal movement of the shake flask, the through holes 311 below the windward space can be filled with air, the air pressure in the shake flask is slightly increased, and therefore the air in the shake flask can flow out from the through holes 311 below the leeward space, the air outside the shake flask and the air inside the shake flask can be continuously and efficiently circularly exchanged, the shake flask can keep high oxygen concentration and low metabolic waste gas concentration, a structure which can not bring shear force to bacteria liquid can not be damaged due to the shear force in the shaking table culture process.

The above disclosure is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the scope of the invention, therefore, the invention is not limited thereto.

Claims (10)

1. The utility model provides an aerobic microorganism liquid culture shake flask, characterized in that, includes bottle, bottle lid and cassette, the bottle has the bottleneck, the bottle lid closes the bottleneck, the cassette is located between the bottleneck with the bottle lid, the bottle lid includes the somatic part and protruding locate the somatic part keeps away from the portion that keeps out the wind of bottle one side, the somatic part is equipped with 4 at least through-holes, the portion that keeps out the wind includes a plurality of adjacent two the portion that keeps out the wind between the through-hole.

2. A liquid culture shake flask for aerobic microorganisms according to claim 1 wherein the flask is a triangular flask.

3. The aerobic microbial liquid culture shake flask according to claim 1, wherein the flask is provided with a volume scale marking.

4. The aerobic microbial liquid culture shake flask according to claim 1, wherein the flask is screw-fit or snap-fit with the cap.

5. The aerobic microbial liquid culture shake flask according to claim 1 wherein the flask comprises an integrally formed neck and body, the neck having an inner diameter that is the same.

6. An aerobic microbial liquid culture flask according to claim 1, wherein the cap is made of plastic or silica gel.

7. A liquid culture shake flask for aerobic microorganisms according to claim 1, wherein the filter is made of a microporous gas permeable material.

8. The aerobic microbial liquid culture shake flask according to claim 1, wherein the body part and the wind shield part are integrally formed.

9. The aerobic microbial broth culture shake flask according to claim 1, wherein the body part is provided with 4 through holes, the wind-shielding part comprises 4 wind-shielding sheets, and the 4 wind-shielding sheets are perpendicular to each other.

10. The aerobic microbial liquid culture shake flask according to claim 9, wherein 4 of the wind-shield sheets are integrally formed.

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