CN219824175U - Culture container sealing structure for phage culture - Google Patents
Culture container sealing structure for phage culture Download PDFInfo
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- CN219824175U CN219824175U CN202321188708.5U CN202321188708U CN219824175U CN 219824175 U CN219824175 U CN 219824175U CN 202321188708 U CN202321188708 U CN 202321188708U CN 219824175 U CN219824175 U CN 219824175U
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- film
- culture
- phage
- sealing
- sealing structure
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- 238000007789 sealing Methods 0.000 title claims abstract description 72
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- -1 polytetrafluoroethylene Polymers 0.000 claims abstract description 8
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims abstract description 8
- 239000004810 polytetrafluoroethylene Substances 0.000 claims abstract description 8
- 239000000835 fiber Substances 0.000 claims abstract description 6
- 229920000915 polyvinyl chloride Polymers 0.000 claims abstract description 6
- 239000004800 polyvinyl chloride Substances 0.000 claims abstract description 6
- 239000012528 membrane Substances 0.000 claims description 17
- 241000700605 Viruses Species 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract description 5
- 230000000903 blocking effect Effects 0.000 abstract description 4
- 238000009423 ventilation Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000589970 Spirochaetales Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 239000002657 fibrous material Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000002655 kraft paper Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000123 paper Substances 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of phage culture, and discloses a sealing structure of a culture container for phage culture, which comprises a culture bottle, wherein a sealing structure is arranged at an opening at the top end of the culture bottle, an elastic band is sleeved outside the sealing structure, the sealing structure comprises a sealing film, the sealing film is formed by combining an upper film, a middle film and a lower film which are sequentially distributed from top to bottom, the upper film is covered and laid on the upper surface of the middle film, the lower film is covered and laid on the lower surface of the middle film, the upper film is used for preventing viruses from entering and exiting, the material is breathable and resistant to high temperature, the middle film is a coarse water-absorbing fiber and used for preventing water vapor generated during phage culture for a long time from blocking polytetrafluoroethylene ventilation holes on the upper layer, so that the normal growth of phage is prevented from being influenced, and the lower film is antistatic polyvinyl chloride and is used for preventing phage from being adsorbed on the sealing film and from being diffused into the environment.
Description
Technical Field
The utility model relates to the technical field of phage culture, in particular to a sealing structure of a culture container for phage culture.
Background
Phage is a generic name of viruses infecting microorganisms such as bacteria, fungi, algae, actinomycetes or spirochetes, is genetic material endowed with biological properties of host bacteria, must be parasitic in living bacteria, has strict host specificity, and is widely applied to the fields of molecular biological research, bacterial identification and typing, detection and control of pathogenic bacteria and the like, and the phage needs to be cultured in a culture container in the biological research process.
The culture container of the existing phage colony mainly has the following problems: the long-time culture of phage in the shaking table easily causes aerosol pollution of phage, and can cause great interference and loss to the scientific research and production work of phage, and at present, a device for sealing a incubator also exists on the market, but after the sealing device is used for a long time, the water vapor generated in the phage culture process is difficult to discharge, and the normal growth of phage is affected.
Disclosure of Invention
The utility model aims to provide a sealing structure of a culture container for phage culture, which solves the problem that water vapor generated in the phage culture process is difficult to discharge and the normal growth of phage is affected.
The utility model provides the following technical scheme: a cultivate container seal structure for phage culture, including the blake bottle, blake bottle top end opening part sets up seal structure, seal structure outside cover is equipped with the rubber band, seal structure includes the sealing membrane, the sealing membrane is formed by the combination of upper membrance, middle membrance and the lower floor's membrane that distributes down in proper order from last, the upper membrance cover is laid in the middle membrance upper surface, the lower floor's membrance cover is laid in the middle membrance lower surface.
As the preferable choice of the technical proposal, the upper layer film is made of polytetrafluoroethylene material, the middle layer film is made of coarse water absorption fiber material, and the lower layer film is made of antistatic polyvinyl chloride material.
As the preferable choice of above-mentioned technical scheme, seal structure still includes the carrier film, a plurality of openings have been seted up to the array on the carrier film, the carrier film is fixed in the opening inside through the tear strip.
As a preferable aspect of the above-described aspect, the plurality of openings are different in size for accommodating sealing films of different sizes.
Preferably, the sealing film has a circular structure, and the diameter of the sealing film is larger than the outer diameter of the culture flask.
As a preferable mode of the above technical scheme, the upper layer film and the lower layer film are both thinner than the thickness of the middle layer film.
Compared with the prior art, the utility model has the beneficial effects that:
in the utility model, the upper layer film is used for preventing viruses from entering and exiting, the material is breathable and high-temperature resistant, the middle layer film is a coarse water absorbing fiber and is used for preventing water vapor generated during phage cultivation for a long time from blocking polytetrafluoroethylene ventilation holes on the upper layer, thereby avoiding influencing normal growth of phage, and the lower layer film is antistatic polyvinyl chloride and is used for preventing phage from being adsorbed on the sealing film and avoiding phage from diffusing from the sealing film to the environment.
Drawings
FIG. 1 is a schematic diagram of an explosive structure of a sealing structure of a culture container for phage culture;
FIG. 2 is a schematic diagram showing the structure of a closure of a culture vessel for phage culture in elevation;
FIG. 3 is a schematic diagram of a connection structure between a base film and a sealing film;
FIG. 4 is an enlarged view of a portion of FIG. 3 at A;
fig. 5 is a schematic view of the internal distribution structure of the sealing film.
In the figure: 10. a culture bottle; 11. an elastic band; 20. sealing film; 21. an upper layer film; 22. a middle layer film; 23. a lower layer film; 30. a base film; 31. an opening; 32. and (5) tearing the strip.
Detailed Description
The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model.
Example 1
As shown in fig. 1-5, the present utility model provides a technical solution: a culture container seal structure for phage culture, including blake bottle 10, blake bottle 10 top opening department sets up seal structure, and seal structure outside cover is equipped with rubber band 11, and seal structure includes seal membrane 20, and seal membrane 20 is formed by the combination of upper film 21, middle layer membrane 22 and lower layer membrane 23 that distribute from top to bottom in proper order, and upper film 21 covers to lay in middle layer membrane 22 upper surface, and lower layer membrane 23 covers to lay in middle layer membrane 22 lower surface.
In the specific use process, firstly, the center of the sealing film 20 is aligned with the center of the port of the culture flask 10 for placement, then the edge of the sealing film 20 is folded downwards, and then the bottle mouth is sleeved with the sealing film 20 from top to bottom through the rubber band 11, so that the sealing film 20 is fixed on the culture flask 10, and when the sealing film 20 is used, the lower surface of the sealing film 20 is always in contact with the upper surface of the culture flask 10, so that a gap is avoided between the sealing film 20 and the culture flask 10.
The upper film 21 is made of polytetrafluoroethylene, and is permeable to air and resistant to high temperatures, and is used for preventing viruses from entering and exiting. The middle layer 22 is a coarse water absorbing fiber, and is used for preventing water vapor generated during long-time phage culture from blocking the polytetrafluoroethylene air holes on the upper layer, so as to avoid influencing the normal growth of phage. The lower film 23 is antistatic polyvinyl chloride for preventing phage from being adsorbed onto the sealing film 20 and preventing phage from diffusing from the sealing film 20 into the environment.
It should be noted that, the sealing film 20 is of a circular structure, and the diameter of the sealing film 20 is larger than the outer diameter of the culture flask 10, so that the sealing film 20 can be folded after being placed at the port of the culture flask 10, so that the edge of the sealing film 20 can be attached to the outer wall of the top of the culture flask 10, and the sealing film 20 can be bound by the rubber band 11.
It should be noted that, the upper layer film 21 and the lower layer film 23 are thinner than the middle layer film 22, so that the sealing film 20 is thinner, the thickness of the water absorbing fiber of the middle layer film 22 is equivalent to that of kraft paper, and the thickness of the upper layer film 21 and the lower layer film 23 are smaller than that of the middle layer film 22, thereby satisfying the use requirement and saving the cost.
Example 2
As shown in fig. 3 to 4, based on the same concept as that of the above-described embodiment 1, the present embodiment also proposes a sealing structure of a culture container for phage culture, the sealing structure further comprising a base film 30, a plurality of openings 31 being formed in an array on the base film 30, and the sealing film 20 being fixed inside the openings 31 by means of a tear strip 32.
In a specific use process, the bottom film 30 is used for placing a plurality of sealing films 20, when the sealing films 20 are not used, the sealing films 20 are all placed on the bottom film 30, when in use, the sealing films 20 are connected with the bottom film 30 through the tear strips 32, so that the sealing films 20 can be separated from the bottom film 30 by manually pressing the sealing films 20, the sealing films 20 are convenient to take when in use, and transparent film papers are arranged on two sides of the bottom film 30, so that impurities are prevented from being stained on the outer surfaces of the sealing films 20.
The plurality of openings 31 are different in size and are adapted to different sizes of sealing films 20, and thus different sizes of sealing films 20 can be placed due to the different sizes of the openings 31, and the sealing films 20 can seal the culture bottles 10 of different sizes.
Working principle: firstly, the center of the sealing film 20 is aligned with the center of the port of the culture flask 10, then the edge of the sealing film 20 is folded downwards, and then the bottle mouth is sleeved with the sealing film 20 from top to bottom through the rubber band 11, so that the sealing film 20 is fixed on the culture flask 10, and when the sealing film 20 is used, the lower surface of the sealing film 20 is always contacted with the upper surface of the culture flask 10, so that a gap is avoided between the sealing film 20 and the culture flask 10. Wherein, the upper layer film 21 is polytetrafluoroethylene for preventing virus from entering and exiting, and the material is breathable and high temperature resistant. The middle layer 22 is a coarse water absorbing fiber, and is used for preventing water vapor generated during long-time phage culture from blocking the polytetrafluoroethylene air holes on the upper layer, so as to avoid influencing the normal growth of phage. The lower film 23 is antistatic polyvinyl chloride for preventing phage from being adsorbed onto the sealing film 20 and preventing phage from diffusing from the sealing film 20 into the environment.
The above embodiments are only for illustrating the technical solution of the present utility model, and are not limiting.
Claims (6)
1. A culture container seal structure for phage culture, including blake bottle (10), its characterized in that: the utility model discloses a culture flask, including culture flask (10), seal structure outside cover is equipped with rubber band (11), seal structure includes seal membrane (20), seal membrane (20) are formed by the combination of upper film (21), middle layer membrane (22) and lower floor's membrane (23) that from last down distributes in proper order, upper film (21) cover is laid in middle layer membrane (22) upper surface, lower floor's membrane (23) cover is laid in middle layer membrane (22) lower surface.
2. The culture container sealing structure for phage culture according to claim 1, wherein: the upper layer film (21) is made of polytetrafluoroethylene, the middle layer film (22) is made of coarse water absorbing fiber, and the lower layer film (23) is made of antistatic polyvinyl chloride.
3. The culture container sealing structure for phage culture according to claim 1, wherein: the sealing structure further comprises a bottom film (30), a plurality of openings (31) are formed in the bottom film (30) in an array mode, and the sealing film (20) is fixed inside the openings (31) through tearing strips (32).
4. A culture vessel sealing structure for phage culture according to claim 3, wherein: the plurality of openings (31) are different in size and are used for adapting to sealing films (20) with different sizes.
5. The culture container sealing structure for phage culture according to claim 1, wherein: the sealing film (20) is of a circular structure, and the diameter of the sealing film (20) is larger than the outer diameter of the culture bottle (10).
6. The culture container sealing structure for phage culture according to claim 1, wherein: the upper layer film (21) and the lower layer film (23) are thinner than the middle layer film (22).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321188708.5U CN219824175U (en) | 2023-05-17 | 2023-05-17 | Culture container sealing structure for phage culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321188708.5U CN219824175U (en) | 2023-05-17 | 2023-05-17 | Culture container sealing structure for phage culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219824175U true CN219824175U (en) | 2023-10-13 |
Family
ID=88272323
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321188708.5U Active CN219824175U (en) | 2023-05-17 | 2023-05-17 | Culture container sealing structure for phage culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219824175U (en) |
-
2023
- 2023-05-17 CN CN202321188708.5U patent/CN219824175U/en active Active
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