CN112094733A - A aseptic closing device that breathes freely of portable for laboratory tissue culture container - Google Patents
A aseptic closing device that breathes freely of portable for laboratory tissue culture container Download PDFInfo
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- CN112094733A CN112094733A CN202011118443.2A CN202011118443A CN112094733A CN 112094733 A CN112094733 A CN 112094733A CN 202011118443 A CN202011118443 A CN 202011118443A CN 112094733 A CN112094733 A CN 112094733A
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- clamping groove
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/24—Gas permeable parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/26—Constructional details, e.g. recesses, hinges flexible
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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Abstract
A portable sterile breathable sealing device for a tissue culture container in an experiment room is characterized in that a membrane body is formed by concentrically connecting a first clamping groove body, a second clamping groove body and a shielding body, the first clamping groove body is an integrated structure formed by a first plane body and a first flange of the first plane body facing downwards in the circumferential direction, a first through hole is formed in the first plane body, a first clamping groove is formed between the first plane body and the first flange, a microbial filtering membrane and a clamping pad are sequentially arranged in the first clamping groove from top to bottom, the second clamping groove body is arranged outside the first flange, the second clamping groove body is an integrated structure formed by a second plane body and a second flange of the second plane body facing downwards in the circumferential direction, a second clamping groove is formed between the second plane body and the second flange, a second through hole is formed in the second plane body, and the shielding body is arranged outside the second flange in the circumferential direction; the sealing device has the advantages of simple structure, scientific and reasonable design, convenient operation, repeated use, greatly reduced sealing time, improved working efficiency, reduced sealing cost and remarkable social and economic benefits.
Description
Technical Field
The invention relates to a breathable sealing film, in particular to a portable sterile breathable sealing device for a tissue culture container in a laboratory.
Background
In the aseptic operation in laboratory, often need utilize tissue culture container to carry out sterilization process to the culture medium, including the later stage to the culture medium inoculate and shake flask culture etc. in order to avoid external interference that has the fungus environment, the tissue culture container need seal the processing, seal commonly used now is with once only ventilative sealing membrane parcel bottleneck after, prick the rubber band in order to realize the state of sealing in bottleneck department, some culture medium need place for a long time, still need cover one deck kraft paper (or newspaper) at ventilative sealing membrane skin, then prick the rubber band in bottleneck department again and seal, consequently need prepare kraft paper (or newspaper) in advance, it is tailor to suitable size spare with it, this process is loaded down with trivial details, and take time. When the culture medium in the tissue culture container is utilized for inoculation culture in the later stage, the rubber band is required to be opened firstly, the breathable sealing film is opened again, the bacteria liquid is added, the breathable sealing film is covered again, then the rubber band is tied up at the bottleneck, time and labor are consumed for repeatedly tying up, the operation is complex, the working efficiency is greatly reduced, the elasticity of the general rubber band is reduced after high-pressure sterilization, the service life is short, the general rubber band is easy to break, the rubber band needs to be replaced with a new one, the workload is increased, the experiment cost is increased, and then, whether the breathable sealing film is available or not can meet the requirement of convenient taking and unloading, the breathable sealing film can be repeatedly used, the working efficiency can be improved, but no public report is seen so.
Disclosure of Invention
In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a portable sterile air-permeable sealing device for a laboratory tissue culture container, which can effectively solve the problems of inconvenient sealing operation, time and labor consumption and low working efficiency.
In order to achieve the purpose, the technical scheme of the invention is that the portable sterile breathable sealing device for the laboratory tissue culture container comprises a membrane body, wherein the membrane body is formed by concentrically connecting a first clamping groove body at the upper part, a second clamping groove body at the middle part and a shielding body at the lower part, the first clamping groove body is an integral structure formed by a first plane body and a first flange of the first plane body which is bent downwards in the circumferential direction in an arc surface manner, a first through hole is formed in the first plane body, a first clamping groove is formed between the first plane body and the first flange, a microorganism filtering membrane and a circular clamping pad used for clamping the microorganism filtering membrane are sequentially arranged in the first clamping groove from top to bottom, an integral second clamping groove body is arranged outside the transverse part of the first flange, and the second clamping groove body is an integral structure formed by the second plane body and a second flange of the second plane body which is bent downwards in the circumferential direction in an arc surface manner, a second clamping groove is formed between the second plane body and the second flange, a second through hole is formed in the second plane body, the diameter D of the second through hole is larger than the diameter D of the first flange, the first through hole, the microbial filtration membrane, the clamping pad, a channel between the first flanges and the second through hole form a concentric ventilation channel, and a shielding body with a downward opening and a skirt shape is circumferentially arranged outside the transverse portion of the second flange.
The invention has simple structure, scientific and reasonable design, convenient operation and repeated use, greatly reduces the sealing time, improves the working efficiency, reduces the sealing cost and has remarkable social and economic benefits.
Drawings
Fig. 1 is a perspective view of the present invention.
Fig. 2 is a front view of the present invention.
Fig. 3 is a sectional view taken along line a-a of fig. 1 in accordance with the present invention.
Fig. 4 is a bottom view of the present invention.
Fig. 5 is a state diagram of the use of the present invention.
Detailed Description
The following detailed description of the embodiments of the invention is provided in connection with the accompanying drawings and the detailed description.
The portable sterile breathable sealing device for the laboratory tissue culture container comprises a film body, wherein the film body is formed by concentrically connecting a first clamping groove body 2 at the upper part, a second clamping groove body 3 at the middle part and a shielding body 4 at the lower part, the first clamping groove body 2 is an integral structure formed by a first plane body 202 and a first flange 201 of the first plane body 202 which is bent downwards in an arc-shaped circumferential direction, a first through hole 1 is formed in the first plane body 202, a first clamping groove 2-1 is formed between the first plane body 202 and the first flange 201, a microorganism filtering membrane 5 and a circular clamping pad 6 used for clamping the microorganism filtering membrane 5 are sequentially arranged in the first clamping groove 2-1 from top to bottom, an integral second clamping groove body 3 is arranged outside the transverse part of the first flange 201, the second clamping groove body 3 is an integral structure formed by a second plane body 302 and a second flange 301 of the second plane body 302 which is bent downwards in an arc-shaped circumferential direction, a second clamping groove 3-1 is formed between the second plane body 302 and the second flange 301, a second through hole 7 is formed in the second plane body 302, the diameter D of the second through hole 7 is larger than the diameter D of the first flange 201, the first through hole 1, the microbial filtering membrane 5, the clamping pad 6, a channel between the first flange 201 and the second through hole 7 form a concentric air-permeable channel, and a shielding body 4 with a downward opening and a skirt shape is circumferentially arranged outside the transverse part of the second flange 301.
For guaranteeing better implementation effect, microbial filtration membrane 5 for the circular that PTFE microbial filtration membrane made, utilize the expanded tensile micropore main part network structure that obtains of low molecular weight PTFE resin, the percent opening reaches more than 85%, aperture 0.3 mu m, can be able to bear 300 ℃ high temperature, can completely cut off bacterium, fungi and some other microorganisms, moisture and air can pass through, effectively completely cut off the external pollution source, have stronger elasticity and hydrophobicity, guaranteed the gas permeability and the sterile environment of tissue culture container.
The diameter of the first through hole 1 is smaller than the diameter d of the first flange 201, and the diameter d of the first flange 201 is smaller than the inner diameter of the clamping pad 6.
First card cell body 2, second card cell body 3 and shelter from body 4 for the integrative structure that 220 ℃ of resistant high temperature silica gel made, shelter from 4 lower parts openings and be located tissue culture ware middle and lower parts, covered most tissue culture ware, reduced the production of microorganism and avoided external microorganism's pollution.
The clamping pad 6 is in a circular ring shape made of silica gel, the lower surface of the clamping pad 6 is in contact with the upper surface of the transverse part of the first flange 201, and the upper surface of the clamping pad is in contact with the lower surface of the microorganism filtering membrane 5, so that the microorganism filtering membrane 5 is fixed in the first clamping groove 2-1.
When the invention is used, taking a 250ml triangular flask in a tissue culture device as an example, the inner diameter of a first clamping groove body 2 is 3.4cm, the groove depth is 0.4cm, the inner diameter of a second clamping groove body 3 is 5.8cm, the groove depth is 0.6cm, the inner diameter of an opening (namely the maximum inner diameter position) at the lower part of a shielding body 4 is 7.3cm, the diameter of a microorganism filtering membrane 5 is 3.33cm, the outer diameter of a clamping pad 6 is 3.38cm, and the inner diameter is 3.08cm, when the invention is used, an experimenter pulls the shielding body 4 part by hand and clamps a second clamping groove 3 downwards into the mouth of the tissue culture container, the two parts are tightly jointed to realize the sealing of the tissue culture container, when the shielding body 4 part is dismounted, the second clamping groove 3 is upwards separated from the mouth of the tissue culture container by hand, the whole structure of the invention is rich in elasticity, is not deformed by pulling, the invention can be repeatedly used, when the microorganism filtering membrane 5 has too many microorganisms or has a damage phenomenon, the clamping pad, the microbial filtration membrane 5 is replaced.
The invention has simple structure, scientific and reasonable design, convenient operation, repeated use and reduced sealing time, through field application and test, multiple persons seal 10 tissue cultivators for multiple times, the sealing time of the existing device is averagely 164s, the sealing time of the invention is averagely 30s, 5 times of time is saved, the working efficiency is greatly improved, rubber band and kraft paper (or newspaper) are saved, the invention is environment-friendly and economical, the sealing cost is reduced, and the invention has obvious social and economic benefits.
Claims (5)
1. A portable sterile breathable sealing device for a tissue culture container in a laboratory comprises a film body and is characterized in that the film body is formed by concentrically connecting a first clamping groove body (2) at the upper part, a second clamping groove body (3) at the middle part and a shielding body (4) at the lower part together, the first clamping groove body (2) is an integrated structure formed by a first plane body (202) and a first flange (201) of the first plane body (202) which is bent downwards in an arc surface shape in the circumferential direction, a first through hole (1) is formed in the first plane body (202), a first clamping groove (2-1) is formed between the first plane body (202) and the first flange (201), a microorganism filtering membrane (5) and a circular clamping pad (6) used for clamping and fixing the microorganism filtering membrane (5) are sequentially arranged in the first clamping groove (2-1) from top to bottom, the second clamping groove body (3) is arranged outside the transverse part of the first flange (201), the second clamping groove body (3) is an integral structure formed by a second plane body (302) and a second flange (301) which is formed by bending the second plane body (302) in an arc surface downward in the circumferential direction, a second clamping groove (3-1) is formed between the second plane body (302) and the second flange (301), a second through hole (7) is formed in the second plane body (302), the diameter D of the second through hole (7) is larger than the diameter D of the first flange (201), the first through hole (1), the microorganism filtering membrane (5), the clamping pad (6), a channel between the first flange (201) and the second through hole (7) form a concentric air-permeable channel, and a shielding body (4) with a downward skirt-shaped opening is arranged in the circumferential direction of the outer side of the second flange (301).
2. The portable sterile gas-permeable sealing device for laboratory tissue culture vessels according to claim 1, characterized in that the microbiological filter membrane (5) is circular made of PTFE.
3. The portable sterile gas-permeable closure for laboratory tissue culture vessels according to claim 1, characterized in that the diameter of the first through hole (1) is smaller than the diameter d of the first flange (201), the diameter d of the first flange (201) being smaller than the inner diameter of the clamping pad (6).
4. The portable sterile air-permeable sealing device for laboratory tissue culture containers according to claim 1, wherein the first clamping groove body (2), the second clamping groove body (3) and the shielding body (4) are of an integrated structure made of silica gel resistant to high temperature of 220 ℃.
5. The portable sterile air-permeable sealing device for laboratory tissue culture containers according to claim 1, characterized in that the clamping pad (6) is in a ring shape made of silica gel, the lower surface of the clamping pad (6) is in contact with the upper surface of the transverse part of the first flange (201), and the upper surface of the clamping pad is in contact with the lower surface of the microbial filtration membrane (5) for fixing the microbial filtration membrane (5) in the first clamping groove (2-1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011118443.2A CN112094733A (en) | 2020-10-19 | 2020-10-19 | A aseptic closing device that breathes freely of portable for laboratory tissue culture container |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011118443.2A CN112094733A (en) | 2020-10-19 | 2020-10-19 | A aseptic closing device that breathes freely of portable for laboratory tissue culture container |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112094733A true CN112094733A (en) | 2020-12-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011118443.2A Pending CN112094733A (en) | 2020-10-19 | 2020-10-19 | A aseptic closing device that breathes freely of portable for laboratory tissue culture container |
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| CN (1) | CN112094733A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115594130A (en) * | 2022-12-13 | 2023-01-13 | 成都圣恩生物科技股份有限公司（Cn） | Automatic bottle opening packaging device and packaging method |
-
2020
- 2020-10-19 CN CN202011118443.2A patent/CN112094733A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115594130A (en) * | 2022-12-13 | 2023-01-13 | 成都圣恩生物科技股份有限公司（Cn） | Automatic bottle opening packaging device and packaging method |
| CN115594130B (en) * | 2022-12-13 | 2023-03-10 | 成都圣恩生物科技股份有限公司 | Automatic bottle opening packaging device and packaging method |
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