CN106906139A - A kind of aerosol tissue culture device - Google Patents
A kind of aerosol tissue culture device Download PDFInfo
- Publication number
- CN106906139A CN106906139A CN201710323950.1A CN201710323950A CN106906139A CN 106906139 A CN106906139 A CN 106906139A CN 201710323950 A CN201710323950 A CN 201710323950A CN 106906139 A CN106906139 A CN 106906139A
- Authority
- CN
- China
- Prior art keywords
- bottle
- aerosol
- tissue culture
- culture device
- mozzle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
- C12M27/24—Draft tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
Abstract
The invention discloses a kind of aerosol tissue culture device, including bottle cap, bottle, bearer network, mozzle, supersonic generator and pedestal, the bottle cap and bottle constitute aerosol receiving space, the bottle cap is provided with gas-filtering device, its inner space is divided into two parts by the bearer network inside bottle, the mozzle is fixed on bottle body bottom through bearer network, and the supersonic generator for being fixed on pedestal discharges diffusion by the nutrient liquid atomizing in bottle and by mozzle.Aerosol tissue culture device of the invention, dissolved oxygen is more in droplet, enhances gas exchanges, and the plant of cultivation is more healthy and stronger, Carbon Dioxide in Air is passed through in incubation, be conducive to plant that external environment is faster adapted to during domestication, humidity is not up to 100% humidity in solid culture and the such bottle of Liquid Culture in bottle, plant stomata function is more sound, reduce vitrifying plant, and aerosol culture is used, it is cost-effective without agar or gel.
Description
Technical field
The present invention relates to a kind of laboratory apparatus, and in particular to a kind of tissue culture device.
Background technology
In method for plant tissue culture, Liquid Culture has nutrient utilization rate higher with respect to solid culture, and plant increases
Grow rapid, the advantage such as growth is fast, stalwartness, but, Liquid Culture ventilation is bad, to be shaken, and often vitrifying is serious, in recent years
Although the immersion culture for growing up solves the problems, such as gas exchanges to a certain extent, however it remains dissolved oxygen content is few, cut
Shear force is big to culture detrimental effect, and aerosol tissue cultures are widely used in the research of biological phenomenon, and gas exchanges are good, pollution rate
It is low, it is the direction of following plant tissue culture production.
The content of the invention
Goal of the invention：The purpose of the present invention is to solve the shortcomings of the prior art, there is provided a kind of aerosol tissue culture device,
Improve culture effect.
Technical scheme：A kind of aerosol tissue culture device of the present invention, including bottle cap, bottle, bearer network, water conservancy diversion
Pipe, supersonic generator and pedestal, the bottle cap and bottle constitute aerosol receiving space, and the bottle cap is provided with gas filtration dress
Put, its inner space is divided into two parts by the bearer network inside bottle, and the mozzle is fixed on bottle through bearer network
Body bottom, the supersonic generator for being fixed on pedestal discharges diffusion by the nutrient liquid atomizing in bottle and by mozzle.
Preferably, the gas-filtering device includes filter membrane and the straight-through component of gas filtration threading, and the filter membrane is located at bottle
At the center through hole of lid, the straight-through component of the gas filtration threading is at the perforation of center through hole periphery.
Preferably, the straight-through component of the gas filtration threading is straight-through including screw thread threading, screw thread threading is arranged in successively straight
Logical the first silica gel pad, the second silica gel pad and locking nut, silicone tube that the straight-through coupling of screw thread threading is sequentially connected and
Online biofilter, the first silica gel pad, the second silica gel pad located at perforation both sides, by locking nut by gas filtration
The straight-through component of threading is fixed on bottle cap.
Preferably, the bottle cap is at least equipped with the straight-through component of more than two gas filtration threadings.
Preferably, the bottle side wall is provided with kink in the diagonal and in axial direction extends contraction flow region, the bottle
Internal bearer network is snapped in kink.
Preferably, the top of the mozzle is provided with capping, and the bottom of mozzle is provided with fence, the water conservancy diversion tube side wall position
The opening for droplet diffusion is offered between top and bearer network.
Preferably, the bottle body bottom is provided with the annular groove of depression, and the mozzle bottom is fixed in annular groove.
Preferably, the pedestal also includes ultrasonic wave fixed mount and ultrasonic piece fastener, and the supersonic generator is located at
On ultrasonic wave fixed mount, and pedestal is fixed on by ultrasonic piece fastener.
Preferably, also including control system, the control system is the timer for controlling supersonic generator to start the time.
Preferably, the bottle cap and bottle, bottle are equipped with close with pedestal and supersonic generator with pedestal junction
Seal.
Beneficial effect：The tissue culture effect of aerosol tissue culture device of the invention is good, Liquid Culture using concussion or
Shaking table waggle formula can all produce shearing force, be unfavorable for the growth of culture, and dissolved oxygen content is very low in nutrient solution, phase
, without shearing force, dissolved oxygen is more in droplet for aerosol tissue cultures than under, is conducive to the growth and development of culture, aerosol culture association
Liquid and gas contradiction is adjusted, gas exchanges have been enhanced, the plant of cultivation is more healthy and stronger, and breeding potential is high, and sky is passed through in incubation
Carbon dioxide in gas, can carry out carbonless base paper, be conducive to plant that external environment is faster adapted to during domestication, humidity in bottle
100% humidity in solid culture and the such bottle of Liquid Culture is not up to, plant stomata function is more sound, reduces vitrifying and plant
Strain, and aerosol culture is used, it is cost-effective without agar or gel.
Brief description of the drawings
Fig. 1 is structural representation of the invention；
Fig. 2 is sectional view of the invention；
Fig. 3 is three-dimensional cutaway view of the invention；
Fig. 4 is that gas filtration threading of the present invention leads directly to component diagram：
Fig. 5 is mozzle schematic diagram of the present invention.
Specific embodiment
Technical solution of the present invention is described in detail below, but protection scope of the present invention is not limited to the implementation
Example.
As shown in Figure 1 to Figure 3, a kind of aerosol tissue culture device of the invention, including bottle cap 1, bottle 2, bearer network 3,
Mozzle 4, supersonic generator 5 and pedestal 6, bottle cap 1 and bottle 2 constitute aerosol receiving space, and bottle cap 1 is by high temperature resistant PC materials
It is made, volume is 2.0L, autoclave sterilization can be directly carried out with high pressure resistant after sealing, bottle cap 1 is provided with gas filtration dress
Put, its inner space is divided into two parts by bearer network 3 inside bottle 2, bearer network 3 is PC plastic discs, φ 115mm, bottle
The top of body 2 is culture vitellarium, and lower storage nutrient solution, mozzle 4 is fixed on the bottom of bottle 2 through bearer network 3, its top
It is 3cm away from bottle cap 1, is fixed on the ultrasonic wave that the supersonic generator 5 of pedestal 6 produces and by the nutrient liquid atomizing in bottle 2 and leads to
The discharge diffusion of mozzle 4 is crossed, includes the droplet of mixed gas and nutrient solution, overflowed to surrounding by mozzle, declined, be training
Support thing and nutritional ingredient and dissolved oxygen are provided, aerosol culture apparatus also includes control system, and control system is control supersonic generator 5
The timer of startup time, gas-filtering device includes filter membrane and the straight-through component of gas filtration threading, and filter membrane is in bottle cap 1
At the through hole 7 of centre, preferably, central air vents are φ 10mm, 2.2um filter membranes are welded with, as shown in figure 4, gas filtration threading
At the perforation 8 of the periphery of center through hole 7, the straight-through component of gas filtration threading includes screw thread threading straight-through 9 to straight-through component, successively
It is arranged in the first silica gel pad 10, the second silica gel pad 11 and the locking nut 12 of screw thread threading straight-through 9, screw thread threading straight-through 9
The online biofilter 14 of silicone tube 13 and 2.2um that coupling is sequentially connected, the first silica gel pad 10, the second silica gel pad
The straight-through component of gas filtration threading, located at 8 both sides of perforation, bottle cap 1 is fixed on by locking nut 12 by 11, and bottle cap 1 at least wears
There are more than two gas filtration threadings to lead directly to component, the side wall of bottle 2 is provided with kink 13 in the diagonal and in axial direction prolongs
Contraction flow region is stretched out, the bearer network 3 in bottle 2 is snapped in kink 13, as shown in figure 5, the top of mozzle 4 is provided with capping, leads
The bottom of flow tube 4 is provided with fence, and the side wall of mozzle 4 is located at the opening 14 offered between top and bearer network 3 for droplet diffusion,
The bottom of bottle 2 is provided with the annular groove 15 of depression, and the bottom of mozzle 4 is fixed in annular groove 15, and pedestal 6 is also fixed including ultrasonic wave
Frame 16 and ultrasonic piece fastener 17, preferably, solid located at ultrasonic wave from the ultrasonic wave generating sheet supersonic generator 5 of 35W
Determine on frame 16, and pedestal 6, bottle cap 1 and bottle 2, bottle 2 and pedestal 6 and ultrasonic wave are fixed on by ultrasonic piece fastener 17
Generator 5 is equipped with sealing ring with the junction of pedestal 6, prevents nutrient solution droplet from evaporateing into outside device.
Although as described above, having been represented with reference to specific preferred embodiment and having described the present invention, it must not be explained
It is to the limitation of itself of the invention.Under the premise of the spirit and scope of the present invention for not departing from appended claims definition, can be right
Various changes can be made in the form and details for it.
Claims (10)
1. a kind of aerosol tissue culture device, it is characterised in that：Including bottle cap (1), bottle (2), bearer network (3), mozzle
(4), supersonic generator (5) and pedestal (6), the bottle cap (1) and bottle (2) constitute aerosol receiving space, the bottle cap (1)
Gas-filtering device is provided with, its inner space is divided into two parts, the water conservancy diversion by the bearer network (3) inside bottle (2)
Pipe (4) is fixed on bottle (2) bottom through bearer network (3), is fixed on the supersonic generator (5) of pedestal (6) by bottle (2)
Nutrient liquid atomizing and by mozzle (4) discharge diffusion.
2. aerosol tissue culture device according to claim 1, it is characterised in that：The gas-filtering device includes filter membrane
Component is led directly to gas filtration threading, the filter membrane is located at center through hole (7) place of bottle cap (1), and the gas filtration threading is straight
Perforation (8) place of logical component located at center through hole (7) periphery.
3. aerosol tissue culture device according to claim 2, it is characterised in that：The gas filtration threading leads directly to component
(9) are led directly to including screw thread threading, the first silica gel pad (10), second silica gel pad of screw thread threading straight-through (9) are arranged in successively
(11) silicone tube (13) and online bacteriological filter that and locking nut (12), straight-through (9) coupling of screw thread threading is sequentially connected
Device (14), the first silica gel pad (10), the second silica gel pad (11) located at perforation (8) both sides, by locking nut (12) by gas
The straight-through component of body filtering threading is fixed on bottle cap (1).
4. aerosol tissue culture device according to claim 3, it is characterised in that：The bottle cap (1) is at least equipped with two
The straight-through component of gas filtration threading more than individual.
5. aerosol tissue culture device according to claim 1, it is characterised in that：Bottle (2) side wall is provided with oblique
Interior kink (13) simultaneously in axial direction extends contraction flow region, and the bearer network (3) in the bottle (2) is snapped in kink
(13)。
6. aerosol tissue culture device according to claim 1, it is characterised in that：The top of the mozzle (4) is provided with
Capping, the bottom of mozzle (4) is provided with fence, and mozzle (4) side wall is located at and offers confession between top and bearer network (3)
The opening (14) of droplet diffusion.
7. the aerosol tissue culture device according to claim 1 or 6, it is characterised in that：Bottle (2) bottom is provided with recessed
Sunken annular groove (15), mozzle (4) bottom is fixed in annular groove (15).
8. aerosol tissue culture device according to claim 1, it is characterised in that：The pedestal (6) also includes ultrasonic wave
Fixed mount (16) and ultrasonic piece fastener (17), the supersonic generator (5) pass through on ultrasonic wave fixed mount (16)
Ultrasonic piece fastener (17) is fixed on pedestal (6).
9. aerosol tissue culture device according to claim 1, it is characterised in that：Also include control system, the control system
Unite and start the timer of time for control supersonic generator (5).
10. the aerosol tissue culture device according to any one of claim 1,4,5,6,8,9, it is characterised in that：It is described
Bottle cap (1) and bottle (2), bottle (2) are equipped with sealing with pedestal (6) and supersonic generator (5) with pedestal (6) junction
Circle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710323950.1A CN106906139A (en) | 2017-05-08 | 2017-05-08 | A kind of aerosol tissue culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710323950.1A CN106906139A (en) | 2017-05-08 | 2017-05-08 | A kind of aerosol tissue culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106906139A true CN106906139A (en) | 2017-06-30 |
Family
ID=59210950
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710323950.1A Pending CN106906139A (en) | 2017-05-08 | 2017-05-08 | A kind of aerosol tissue culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106906139A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184674A (en) * | 2018-03-14 | 2018-06-22 | 上海电机学院 | A kind of ultrasonic wave device for cultivating plant tissue |
| CN114166257A (en) * | 2021-12-07 | 2022-03-11 | 华北理工大学 | Collecting space area optical fiber sensing monitoring system |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1188593A (en) * | 1997-01-22 | 1998-07-29 | 中国科学院遗传研究所 | Culture container and method for producing potato miniature potato etc. plant material using container thereof |
| CN101953296A (en) * | 2009-07-17 | 2011-01-26 | 中国科学院过程工程研究所 | Pressure pulsation ultrasonic atomization culture device and cultural method using same |
-
2017
- 2017-05-08 CN CN201710323950.1A patent/CN106906139A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1188593A (en) * | 1997-01-22 | 1998-07-29 | 中国科学院遗传研究所 | Culture container and method for producing potato miniature potato etc. plant material using container thereof |
| CN101953296A (en) * | 2009-07-17 | 2011-01-26 | 中国科学院过程工程研究所 | Pressure pulsation ultrasonic atomization culture device and cultural method using same |
Non-Patent Citations (1)
| Title |
|---|
| CHUN-ZHAO LIU等: "Comparison of various bioreactors on growth and artemisinin biosynthesis of Artemisia annua L. shoot cultures" * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184674A (en) * | 2018-03-14 | 2018-06-22 | 上海电机学院 | A kind of ultrasonic wave device for cultivating plant tissue |
| CN114166257A (en) * | 2021-12-07 | 2022-03-11 | 华北理工大学 | Collecting space area optical fiber sensing monitoring system |
| CN114166257B (en) * | 2021-12-07 | 2023-06-20 | 华北理工大学 | Goaf optical fiber sensing monitoring system |
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