CN108184674A - A kind of ultrasonic wave device for cultivating plant tissue - Google Patents
A kind of ultrasonic wave device for cultivating plant tissue Download PDFInfo
- Publication number
- CN108184674A CN108184674A CN201810210603.2A CN201810210603A CN108184674A CN 108184674 A CN108184674 A CN 108184674A CN 201810210603 A CN201810210603 A CN 201810210603A CN 108184674 A CN108184674 A CN 108184674A
- Authority
- CN
- China
- Prior art keywords
- bottle
- ultrasonic
- plant tissue
- shelf
- bottleneck
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to biotechnology more particularly to a kind of ultrasonic wave device for cultivating plant tissue.A kind of ultrasonic wave device for cultivating plant tissue, including bottle, it is characterised in that：Further include the ultrasonic atomization component inside bottle, the shelf for being placed on bottleneck and its bottle cap for sealing；The present invention ensures plant eupnea when absorbing nutriment, and plant tissue is avoided vitrifying occur, improves the emergence rate of Plant Tissue Breeding.
Description
Technical field
The present invention relates to biotechnology more particularly to a kind of ultrasonic wave device for cultivating plant tissue.
Background technology
The plant tissue culture prior art includes solid medium, fluid nutrient medium
Water, machine nutritional ingredient, inorganic salts, agar are mixed in a certain ratio, plant hormone is added in after boiling through cooling down shape
Into solid medium.Plant Tissue Breeding can be carried out on solid medium after autoclave sterilization.
Culture solution is made with water, machine nutritional ingredient, inorganic salts, plant hormone basigamy, can be soaked after sterilized by interval
The method culture plant tissue of bubble.
The poor fluidity of solid medium, nutrition from plant tissue farther out after the nutrition around plant tissue is absorbed
Ingredient can not flow in time in solid-like.Solid medium nutrient concentration after culture a period of time around plant tissue reduces,
Originally plant tissue equally obtains enough nutriments without the image of Buddha, delay the growth in plant tissue later stage significantly.
Fluid nutrient medium good fluidity, but plant tissue is completely soaked in liquid medium within plant tissue can be hindered normal
Respiration lead to plant tissue vitrifying, culture failure.In addition achieve the effect that be completely soaked plant tissue with more
Fluid nutrient medium.
Invention content
The object of the present invention is to provide a kind of ultrasonic wave device for cultivating plant tissue.The present invention seeks to ensure that plant is inhaling
Eupnea when receiving nutriment, avoids plant tissue from vitrifying occur, improves the emergence rate of Plant Tissue Breeding
The present invention is that technical solution is used by solving its technical problem：
A kind of ultrasonic wave device for cultivating plant tissue including bottle, further includes the ultrasonic atomization component inside bottle, puts
Put the shelf in bottleneck and its bottle cap for sealing；
The bottle mouth position of the bottle is provided with screw thread, several branch fasteners are protruding with along internal diameter of the bottleneck inside bottleneck,
The supporting shelf is placed on the top of branch fastener, and there are one line throughholes for the bottom hollow out of the bottle；
The bottle cap is a deep mouth bottle cap, and the bottle cap is connected through a screw thread with bottle, and bottle cap is threadedly coupled with bottle
Place is additionally provided with sealing ring；
The ultrasonic atomization component includes ultrasonic ultrasonic delay line memory, and the ultrasonic ultrasonic delay line memory is mounted on inside bottle
Bottom surface, the ultrasonic ultrasonic delay line memory make bottle with the ultrasonic wave controller connection outside bottle, circuit by line throughhole
Inside and outside connection, the ultrasonic ultrasonic delay line memory are sealed with bottom of bottle by glue envelope.
The shelf size is corresponding to internal diameter of the bottleneck, and 4 gas circuit through-holes are also uniformly opened up at the circumference of shelf.Stomata leads to
Road is used for the taking-up that the culture solution of atomization is made to be also convenient for shelf simultaneously full of entire bottle.
The principle of the present invention is：Liquid medium is added in bottle, is sealed by bottle cap, ultrasonic wave controller control
Ultrasonic ultrasonic delay line memory work processed, is atomized liquid culture solution, flows to entire bottle by the gas circuit through-hole of shelf, make plant and mist
The gas of change comes into full contact with.
The advantage of the invention is that：Fluid nutrient medium is in mist after atomization, and subtle water drop suspension is in air, generally
More than 95% particle is less than 5um, ensures plant eupnea when absorbing nutriment, plant tissue is avoided glass occur
Change, improve the emergence rate of Plant Tissue Breeding.
Fluid nutrient medium does not use agar, it is possible to reduce 40-50% consumables costs.
Fluid nutrient medium good fluidity, solution is uniform, stablizes, and management is facilitated to be suitble to large-scale application.According to different plants
Kind can improve 1-2 times of yield, shorten cultivation period, bottle seedling is strong, uniform in size.
Description of the drawings
Fig. 1 is ultrasonic wave device for cultivating plant tissue schematic diagram of the present invention.
Specific embodiment
In order to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, tie below
Diagram and specific embodiment are closed, the present invention is further explained.
Such as Fig. 1, a kind of ultrasonic wave device for cultivating plant tissue, including bottle 1, it is characterised in that：It further includes inside bottle
Ultrasonic atomization component 2, be placed on bottleneck shelf 4 and its bottle cap 3 for sealing；
The bottle mouth position of the bottle 1 is provided with screw thread, several branch fasteners are protruding with along internal diameter of the bottleneck inside bottleneck
12, the shelf 4 is placed on the top of branch fastener 12, and there are one line throughholes 13 for the bottom hollow out of the bottle 1；
The bottle cap 3 is a deep mouth bottle cap, and the bottle cap 3 is connected through a screw thread with bottle 1, bottle cap 3 and 1 screw thread of bottle
Junction is additionally provided with sealing ring 31；
The ultrasonic atomization component 2 includes ultrasonic ultrasonic delay line memory 21, and the ultrasonic ultrasonic delay line memory 21 is consolidated by screw
It is scheduled on the bottom surface inside bottle 1, the ultrasonic ultrasonic delay line memory 21 and 22 connection of ultrasonic wave controller outside bottle, circuit
Make to connect inside and outside bottle by line throughhole 13, the ultrasonic ultrasonic delay line memory 21 is sealed with bottom of bottle by glue envelope.
4 size of shelf is corresponding to internal diameter of the bottleneck, and 4 gas circuit through-holes 41 are also uniformly opened up at the circumference of shelf 4.
Fluid nutrient medium 5 is added in inside bottle 1, required culture plant is placed on shelf 4, is carried out with bottle cap 3 close
Envelope, is placed under suitable environment and cultivates plant.
Basic principle, main feature and the advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, various changes and improvements may be made to the invention without departing from the spirit and scope of the present invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent defines.
Claims (2)
1. a kind of ultrasonic wave device for cultivating plant tissue, including bottle (1), it is characterised in that：Further include the ultrasound inside bottle
Wave atomizing component (2), the shelf (4) for being placed on bottleneck and its bottle cap (3) for sealing；
The bottle mouth position of the bottle (1) is provided with screw thread, several branch fasteners are protruding with along internal diameter of the bottleneck inside bottleneck
(12), the shelf (4) is placed on the top of branch fastener (12), and there are one line throughholes for the bottom hollow out of the bottle (1)
(13)；
The bottle cap (3) is a deep mouth bottle cap, and the bottle cap (3) is connected through a screw thread with bottle (1), bottle cap (3) and bottle
(1) threaded connection place is additionally provided with sealing ring (31)；
The ultrasonic atomization component (2) includes ultrasonic ultrasonic delay line memory (21), and the ultrasonic ultrasonic delay line memory (21) passes through screw
It is fixed on the internal bottom surface of bottle (1), the ultrasonic ultrasonic delay line memory (21) and ultrasonic wave controller (22) circuit outside bottle
Connection, circuit make bottle is inside and outside to connect by line throughhole (13), the ultrasonic ultrasonic delay line memory (21) and bottom of bottle by glue seal into
Row sealing.
2. ultrasonic wave device for cultivating plant tissue according to claim 1, it is characterised in that：Described shelf (4) size
It is corresponding to internal diameter of the bottleneck, 4 gas circuit through-holes (41) are also uniformly opened up at the circumference of shelf (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810210603.2A CN108184674A (en) | 2018-03-14 | 2018-03-14 | A kind of ultrasonic wave device for cultivating plant tissue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810210603.2A CN108184674A (en) | 2018-03-14 | 2018-03-14 | A kind of ultrasonic wave device for cultivating plant tissue |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108184674A true CN108184674A (en) | 2018-06-22 |
Family
ID=62595842
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810210603.2A Pending CN108184674A (en) | 2018-03-14 | 2018-03-14 | A kind of ultrasonic wave device for cultivating plant tissue |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108184674A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7824914B2 (en) * | 2006-12-29 | 2010-11-02 | Tatung Company | Flavonoids-rich tissues from Neomarica gracilis and methods for culturing the same |
| CN204742125U (en) * | 2015-05-27 | 2015-11-11 | 昆明理工大学 | Plant tissue culture bottle with cultivate board |
| CN106906139A (en) * | 2017-05-08 | 2017-06-30 | 江苏农林职业技术学院 | A kind of aerosol tissue culture device |
| CN207002738U (en) * | 2017-05-08 | 2018-02-13 | 江苏农林职业技术学院 | A kind of aerosol tissue culture device |
| CN207011439U (en) * | 2017-05-12 | 2018-02-16 | 云南新维特生物科技有限公司 | A kind of stem of noble dendrobium blake bottle |
-
2018
- 2018-03-14 CN CN201810210603.2A patent/CN108184674A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7824914B2 (en) * | 2006-12-29 | 2010-11-02 | Tatung Company | Flavonoids-rich tissues from Neomarica gracilis and methods for culturing the same |
| CN204742125U (en) * | 2015-05-27 | 2015-11-11 | 昆明理工大学 | Plant tissue culture bottle with cultivate board |
| CN106906139A (en) * | 2017-05-08 | 2017-06-30 | 江苏农林职业技术学院 | A kind of aerosol tissue culture device |
| CN207002738U (en) * | 2017-05-08 | 2018-02-13 | 江苏农林职业技术学院 | A kind of aerosol tissue culture device |
| CN207011439U (en) * | 2017-05-12 | 2018-02-16 | 云南新维特生物科技有限公司 | A kind of stem of noble dendrobium blake bottle |
Non-Patent Citations (3)
| Title |
|---|
| D. BRICEÑO-GUTIERREZ等: "On the Ultrasonic Atomization of Liquids", 《PHYSICS PROCEDIA》 * |
| 郝震龙等: "应用雾化营养液生物反应器繁殖脱毒马铃薯的研究", 《生物工程学报》 * |
| 高文远: "《中药生物工程》", 31 January 2014, 上海科学技术出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN204762648U (en) | Growing environment controllable soilless culture device | |
| CN204362708U (en) | Ultrasonic aerosol culture apparatus | |
| CN204742124U (en) | Plant tissue culture bottle lid of adjustable throughput | |
| CN203399582U (en) | Chinese chive water planting device | |
| CN207203936U (en) | A kind of efficient recirculated water device for continuously adding medicine | |
| CN107557299B (en) | Liquid atomization system for cell culture | |
| CN108184674A (en) | A kind of ultrasonic wave device for cultivating plant tissue | |
| CN110771491A (en) | High-efficient little fog plant culture apparatus | |
| CN208242510U (en) | A kind of light comparison culture formula plant incubator | |
| CN106399056A (en) | Sterile air breather suitable for alga culture | |
| CN106418123A (en) | Device for pesticide residue degradation and freshness retaining | |
| CN211241199U (en) | High-efficient little fog plant culture apparatus | |
| CN207002738U (en) | A kind of aerosol tissue culture device | |
| CN106906139A (en) | A kind of aerosol tissue culture device | |
| CN213232038U (en) | Novel energy-saving and environment-friendly organic fertilizer raw material aerobic fermentation tank | |
| CN114051919A (en) | Aerial fog type plant sugar-free culture device, control method thereof and plant culture method | |
| CN206602977U (en) | A kind of family oriented ultrasonic fog trains device | |
| CN209359243U (en) | Breeding environment moistening and lowering temperature sterilizes integrated control system | |
| CN111142586B (en) | Control system of aeroponic planting device | |
| CN204860463U (en) | Can be used to add in succession tissue culture bottle of nutrient solution | |
| CN209711028U (en) | A kind of drip irrigation system of tomato fruit chamber planting nutrient solution | |
| CN207944037U (en) | A kind of rice cropping nutrient solution automatic preparation system | |
| CN205821302U (en) | A kind of use for laboratory culture medium packaging device | |
| CN205922300U (en) | Park maintenance uses and drips irrigation device from dynamic formula with medicine | |
| CN206266628U (en) | Culture device for liquid strain of edible fungus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180622 |
|
| RJ01 | Rejection of invention patent application after publication |