CN106399056A - Sterile air breather suitable for alga culture - Google Patents
Sterile air breather suitable for alga culture Download PDFInfo
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- CN106399056A CN106399056A CN201611069301.5A CN201611069301A CN106399056A CN 106399056 A CN106399056 A CN 106399056A CN 201611069301 A CN201611069301 A CN 201611069301A CN 106399056 A CN106399056 A CN 106399056A
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- gas
- pipe
- filter
- triangular flask
- sebific duct
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Cultivation Of Seaweed (AREA)
Abstract
The invention relates to a sterile air breather suitable for alga culture. The sterile air breather comprises an air pump, a flowmeter and a filter, wherein the flowmeter and the filter are connected in series on a gas pipeline, a main gas pipe passing by the filter is communicated with a gas transmission framed bent, a plurality of gas outlet mouths are installed on each vertical gas guide pipe on the gas transmission framed bent, a gas outlet rubber pipe is inserted and connected in a lower port of each gas outlet mouth, a pipe clamp used for controlling on/off of each gas outlet rubber pipe is installed on the gas outlet rubber pipe, a lower port of each gas outlet rubber pipe is in inserted connection with a gas-filling through pipe inserted and installed in a rubber stopper in an opening of a triangular flask, each gas-filling through pipe extends into the corresponding triangular flask after passing through the rubber stopper in the opening of the corresponding triangular flask, an in-flask gas-filling rubber pipe extending to the bottom part of each triangular flask is inserted and connected in a lower port of the corresponding gas-filling through pipe, and meanwhile, an exhaust pipe communicated with the interior and the exterior of each triangular flask is inserted and installed in the corresponding rubber stopper. The sterile culture of the marine microalgae is implemented, the flow of the transmitted gas is controllable, one set of equipment provides multiple groups of culturing at the same time, and the structure of the device also lays a foundation for the large-area industrial aquaculture in future.
Description
Technical field
The invention belongs to algae culture equipment technical field, especially relate to a kind of aseptic ventilation dress for algae culture
Put.
Background technology
Marine microalgae, its contained nutrient substance very abundant, except containing rich in protein, aminoacid, pigment etc.,
Also it is rich in the nutritional labeling of high value and the industrial chemicals such as EPA and DHA.At present, marine microalgae is widely used in pharmaceuticals industry, food
In product industry and animal feed.
In laboratory, the cultivation of marine microalgae is to be cultivated in triangular flask, but this aquaculture model has certain limit
System, gas can only be entered by sealing bottle film, does not reach the CO needed for microalgae2Demand, and utilize air pump injection gas it is impossible to reality
Existing gas asepticization, and then make microalgae microbiological contamination；Therefore, develop a kind of aseptic breather being effectively used for algae culture to have
Important meaning.
Content of the invention
It is an object of the invention to overcoming the deficiencies in the prior art, provide a kind of aseptic ventilation dress for algae culture
Put.
The present invention solves its technical problem and takes technical scheme below to realize：
A kind of aseptic breather for algae culture, this device includes air pump, and the gas outlet of air pump is connected with air pump
Outtake tube, is serially connected with the effusion meter controlling gas flow, in the termination grafting of flowmeter outlet pipe on air pump outtake tube
There are the import sebific duct of filter, the port of import sebific duct and the import mouth grafting of filter, in the inside of filter, filter is installed
Net group, the outlet mouth of filter and the discharge hose grafting of filter, are plugged with gas transmission main in the exterior side port of discharge hose,
Bending is connected with gas transmission framed bent downwards for the outlet side of gas transmission main, and described gas transmission framed bent includes a horizontal airway, horizontal
Even on airway be communicated with one plane many longitudinal airways away from interval, on every longitudinal airway even away from interval
Multiple downward outlet nozzles are installed, are plugged with, in the lower port of each outlet nozzle, sebific duct of giving vent to anger, giving vent to anger, control is installed on sebific duct
Make the pipe clamp of gas sebific duct break-make, the lower port of sebific duct of giving vent to anger is inserted with the inflation poling being inserted in rubber stopper at triangular flask opening
Connect, inflation poling extends in triangular flask after passing through the rubber stopper sealing triangular flask upper shed, insert in the lower port of inflation poling
It is connected to inflation sebific duct in the bottle extend to triangular flask bottom, meanwhile, plug-in mounting connects the exhaustor inside and outside triangular flask on rubber stopper.
And, described filter screen group further includes active carbon layer and the thalline with active carbon layer Alignment at air intake end
Filter membrane, described active carbon layer is used for moisture and large-size particle in adsorbed gas, and described thalline filter membrane is existed using aperture
Filter membrane between 0.20um-0.22um, for filtering the thalline in gas.
And, the gas outlet pressure of described air pump is maintained between 1.05-1.08 atmospheric pressure.
And, described exhaustor passes rubber stopper rearward end and is bent downwardly.
And, described pipe clamp further includes a cuboid folder frame, has threaded wearing on the upper roof of folder frame
Hole, is installed with threaded push rod in perforation, is formed with turncap in the upper end of push rod, is connected with discoid pressure in the lower end of push rod
Plate, rotation turncap is controlled the flow of the sebific duct of giving vent to anger being installed on folder inframe by pressing plate.
Advantages of the present invention and good effect are：
1. the present invention solves the asepticization operation of the ventilation in microalga cultivation process；
2. the present invention can control CO in microalga cultivation process by adjusting effusion meter2Total ventilation；
3. the present invention can control the CO of each triangular flask by adjusting pipe clamp 192Ventilation；
4. the present invention is easy and simple to handle, easy to use.
Brief description
Fig. 1 is the overall structure diagram of the present invention；
Fig. 2 is the mplifying structure schematic diagram of filter in the present invention；
Fig. 3 is the mplifying structure schematic diagram of pipe clamp in the present invention.
Specific embodiment
Below in conjunction with accompanying drawing, the embodiment of the present invention is further described it is emphasized that, implementation below is to say
Bright property, rather than determinate it is impossible in this embodiment as limitation of the invention.
A kind of aseptic breather for algae culture, as shown in figure 1, this device includes air pump 12, the giving vent to anger of air pump
Mouth is connected with air pump outtake tube 11, is serially connected with the effusion meter 10 controlling gas flow, in effusion meter on air pump outtake tube
The termination of outlet is plugged with the import sebific duct 9 of filter, and import mouth 8 grafting of the port of import sebific duct and filter, in mistake
The inside of filter is provided with the filter screen group 7 for filter bacteria, the outlet mouth 6 of filter and discharge hose 5 grafting of filter,
It is plugged with gas transmission main 4 in the exterior side port of discharge hose, bending is connected with gas transmission framed bent downwards for the outlet side of gas transmission main, institute
State gas transmission framed bent and include a horizontal airway 3, even many being communicated with away from interval in one plane on horizontal airway
Longitudinal airway 2, even on every longitudinal airway is provided with multiple downward outlet nozzles 1 away from interval, in each outlet nozzle
Lower port is plugged with sebific duct 16 of giving vent to anger, and installs, on sebific duct, the pipe clamp 19 controlling sebific duct break-make of giving vent to anger giving vent to anger, gives vent to anger under sebific duct
Port and inflation poling 17 grafting being inserted in rubber stopper 14 at triangular flask 15 opening, inflation poling passes through on sealing triangular flask
Extend to after the rubber stopper of opening in triangular flask, be plugged with the bottle extending to triangular flask bottom in the lower port of inflation poling and fill
Gas sebific duct 18, meanwhile, on rubber stopper, plug-in mounting connects the exhaustor 13 inside and outside triangular flask.
In being embodied as of the present invention, as shown in Fig. 2 described filter screen group further includes the active carbon layer at air intake end
20 and the thalline filter membrane 21 with property layer of charcoal Alignment, described active carbon layer is used for moisture and large scale in adsorbed gas
Grain, described thalline filter membrane adopts filter membrane between 0.20um-0.22um for the aperture, for filtering the thalline in gas.
In being embodied as of the present invention, described gas uniform and stable to the inflation of marine microalgae in triangular flask in order to ensure
The gas outlet pressure of pump is maintained between 1.05-1.08 atmospheric pressure, and this pressure is conducive to the steady operation of filter simultaneously,
Be conducive to the physical life of filter screen group.
In being embodied as of the present invention, described exhaustor passes rubber stopper rearward end and is bent downwardly, to avoid in air
Dust fall in exhaustor, pollution triangular flask in marine microalgae.
In being embodied as of the present invention, described pipe clamp further includes a cuboid folder frame 24, on the upper top of folder frame
Threaded perforation is had on wall, perforation is installed with threaded push rod 23, is formed with turncap 22 in the upper end of push rod, on top
The lower end of bar is connected with discshaped platen 25, and rotation turncap is controlled the flow of the sebific duct of giving vent to anger being installed on folder inframe by pressing plate.
In being embodied as of the present invention, described air pump is that carbon dioxide decompression or pressurization are pumped into and are installed pipes
In.
Claims (5)
1. a kind of aseptic breather for algae culture it is characterised in that：This device includes air pump, and the gas outlet of air pump is even
It is connected to air pump outtake tube, the effusion meter controlling gas flow is serially connected with air pump outtake tube, in flowmeter outlet pipe
Termination is plugged with the import sebific duct of filter, and the import mouth grafting of the port of import sebific duct and filter, in the inside of filter
Filter screen group, the outlet mouth of filter and the discharge hose grafting of filter are installed, are plugged with the exterior side port of discharge hose
Gas transmission main, bending is connected with gas transmission framed bent downwards for the outlet side of gas transmission main, and described gas transmission framed bent includes a horizontal inducing QI
Pipe, even many longitudinal airways being communicated with away from interval in one plane on horizontal airway, in every longitudinal airway
Upper even away from interval, multiple downward outlet nozzles are installed, be plugged with, in the lower port of each outlet nozzle, sebific duct of giving vent to anger, in glue of giving vent to anger
Install on pipe and control the pipe clamp of sebific duct break-make of giving vent to anger, the lower port of sebific duct of giving vent to anger be inserted in rubber stopper at triangular flask opening
Inflation poling grafting, inflation poling extends in triangular flask after passing through the rubber stopper sealing triangular flask upper shed, in inflation poling
Lower port be plugged with and extend to inflation sebific duct in the bottle of triangular flask bottom, meanwhile, in plug-in mounting connection triangular flask on rubber stopper
Outer exhaustor.
2. the aseptic breather for algae culture according to claim 1 it is characterised in that：Described filter screen group enters one
Step includes active carbon layer and the thalline filter membrane with active carbon layer Alignment at air intake end, and described active carbon layer is used for adsorbed gas
Moisture in body and large-size particle, described thalline filter membrane adopts filter membrane between 0.20um-0.22um for the aperture, is used for filtering
Thalline in gas.
3. the aseptic breather for algae culture according to claim 1 it is characterised in that：The giving vent to anger of described air pump
Mouth pressure is maintained between 1.05-1.08 atmospheric pressure.
4. the aseptic breather for algae culture according to claim 1 it is characterised in that：Described exhaustor passes
Rubber stopper rearward end is bent downwardly.
5. the aseptic breather for algae culture according to claim 1 it is characterised in that：Described pipe clamp is further
Press from both sides frame including a cuboid, the upper roof of folder frame has threaded perforation, perforation is installed with threaded top
Bar, is formed with turncap in the upper end of push rod, is connected with discshaped platen in the lower end of push rod, and rotation turncap is installed on by pressing plate control
The flow of the sebific duct of giving vent to anger of folder inframe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611069301.5A CN106399056A (en) | 2016-11-29 | 2016-11-29 | Sterile air breather suitable for alga culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611069301.5A CN106399056A (en) | 2016-11-29 | 2016-11-29 | Sterile air breather suitable for alga culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106399056A true CN106399056A (en) | 2017-02-15 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611069301.5A Pending CN106399056A (en) | 2016-11-29 | 2016-11-29 | Sterile air breather suitable for alga culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106399056A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110106064A (en) * | 2019-06-03 | 2019-08-09 | 中国矿业大学 | A kind of microalgae fast screening devices and microalgae rapid screening method |
| CN111592979A (en) * | 2020-06-05 | 2020-08-28 | 鄂尔多斯市琢成生物科技有限责任公司 | Screening device for grading algae body of algae suspension inflation continuous culture |
| CN114208738A (en) * | 2021-12-02 | 2022-03-22 | 重庆绵凯生物技术研究院有限公司 | Method for producing sterile fairy shrimp and application thereof |
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| CN104429878A (en) * | 2013-09-18 | 2015-03-25 | 天津海友佳音生物科技股份有限公司 | Large device for algae suspension aeration culture |
| CN204848863U (en) * | 2015-06-18 | 2015-12-09 | 深圳绿色环球生物科技有限公司 | Bacterial expands banks up container air feeder with earth |
| CN206219570U (en) * | 2016-11-29 | 2017-06-06 | 天津农学院 | For the aseptic air-breather of algae culture |
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2016
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN2786131Y (en) * | 2005-05-25 | 2006-06-07 | 李俊贤 | Liquid strain incubator |
| CN2800004Y (en) * | 2005-06-24 | 2006-07-26 | 曾明 | Rubber tube clip valve |
| CN102242064A (en) * | 2011-05-25 | 2011-11-16 | 中国海洋大学 | Continuous air supplying method and system for chlamydomonas cultivation |
| CN104429878A (en) * | 2013-09-18 | 2015-03-25 | 天津海友佳音生物科技股份有限公司 | Large device for algae suspension aeration culture |
| CN204848863U (en) * | 2015-06-18 | 2015-12-09 | 深圳绿色环球生物科技有限公司 | Bacterial expands banks up container air feeder with earth |
| CN206219570U (en) * | 2016-11-29 | 2017-06-06 | 天津农学院 | For the aseptic air-breather of algae culture |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110106064A (en) * | 2019-06-03 | 2019-08-09 | 中国矿业大学 | A kind of microalgae fast screening devices and microalgae rapid screening method |
| CN111592979A (en) * | 2020-06-05 | 2020-08-28 | 鄂尔多斯市琢成生物科技有限责任公司 | Screening device for grading algae body of algae suspension inflation continuous culture |
| CN114208738A (en) * | 2021-12-02 | 2022-03-22 | 重庆绵凯生物技术研究院有限公司 | Method for producing sterile fairy shrimp and application thereof |
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Application publication date: 20170215 |
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| RJ01 | Rejection of invention patent application after publication |