CN207002738U - A kind of aerosol tissue culture device - Google Patents
A kind of aerosol tissue culture device Download PDFInfo
- Publication number
- CN207002738U CN207002738U CN201720499327.7U CN201720499327U CN207002738U CN 207002738 U CN207002738 U CN 207002738U CN 201720499327 U CN201720499327 U CN 201720499327U CN 207002738 U CN207002738 U CN 207002738U
- Authority
- CN
- China
- Prior art keywords
- bottle
- aerosol
- tissue culture
- mozzle
- culture device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The utility model discloses a kind of aerosol tissue culture device, including bottle cap, bottle, bearer network, mozzle, supersonic generator and pedestal, the bottle cap and bottle form aerosol receiving space, the bottle cap is provided with gas-filtering device, its inner space is divided into two parts by the bearer network inside bottle, the mozzle is fixed on bottle body bottom through bearer network, and the supersonic generator for being fixed on pedestal discharges diffusion by the nutrient liquid atomizing in bottle and by mozzle.Aerosol tissue culture device of the present utility model, dissolved oxygen is more in droplet, enhances gas exchanges, and the plant of cultivation is more healthy and stronger, Carbon Dioxide in Air is passed through in incubation, be advantageous to plant and external environment is faster adapted to during domestication, humidity is not up to 100% humidity in solid culture and the such bottle of Liquid Culture in bottle, and plant stomata function is more sound, reduce vitrifying plant, and aerosol culture is used, without agar or gel, save cost.
Description
Technical field
It the utility model is related to a kind of laboratory apparatus, and in particular to a kind of tissue culture device.
Background technology
In method for plant tissue culture, Liquid Culture has that nutrient utilization rate is higher with respect to solid culture, and plant increases
Grow rapidly, the advantage such as growth is fast, stalwartness, still, Liquid Culture ventilation is bad, to be shaken, often vitrifying is serious, in recent years
Although the immersion culture to grow up solves the problems, such as gas exchanges to a certain extent, however it remains dissolved oxygen content is few, cuts
Shear force is greatly to culture detrimental effect, and aerosol tissue cultures are widely used in the research of biological phenomenon, and gas exchanges are good, pollution rate
It is low, it is the direction of following plant tissue culture production.
Utility model content
Goal of the invention：The purpose of this utility model is in view of the shortcomings of the prior art, there is provided a kind of aerosol tissue cultures
Device, improve culture effect.
Technical scheme：A kind of aerosol tissue culture device described in the utility model, including bottle cap, bottle, bearer network, lead
Flow tube, supersonic generator and pedestal, the bottle cap and bottle form aerosol receiving space, and the bottle cap fills provided with gas filtration
Put, its inner space is divided into two parts by the bearer network inside bottle, and the mozzle is fixed on bottle through bearer network
Body bottom, the supersonic generator for being fixed on pedestal discharge diffusion by the nutrient liquid atomizing in bottle and by mozzle.
Preferably, the gas-filtering device includes filter membrane and gas filtration threading leads directly to component, and the filter membrane is located at bottle
At the center through hole of lid, the gas filtration threading leads directly to component at the perforation of center through hole periphery.
Preferably, the gas filtration threading leads directly to component and led directly to including screw thread threading, and it is straight to be arranged in screw thread threading successively
Logical the first silica gel pad, the second silica gel pad and locking nut, screw thread threading lead directly to the silicone tube that is sequentially connected of coupling and
Online biofilter, the first silica gel pad, the second silica gel pad are located at perforation both sides, by locking nut by gas filtration
Threading leads directly to component and is fixed on bottle cap.
Preferably, the bottle cap is at least equipped with more than two gas filtration threadings and leads directly to component.
Preferably, the bottle side wall is provided with kink in the diagonal and in axial direction extends contraction flow region, the bottle
Internal bearer network is snapped in kink.
Preferably, the top of the mozzle is provided with capping, and the bottom of mozzle is provided with fence, the mozzle side wall position
The opening for droplet diffusion is offered between top and bearer network.
Preferably, the bottle body bottom is provided with the annular groove of depression, and the mozzle bottom is fixed in annular groove.
Preferably, the pedestal also includes ultrasonic wave fixed mount and ultrasonic piece fastener, the supersonic generator are located at
On ultrasonic wave fixed mount, and pedestal is fixed on by ultrasonic piece fastener.
Preferably, in addition to control system, the control system start the timer of time for control supersonic generator.
Preferably, the bottle cap and bottle, bottle are equipped with close with pedestal and supersonic generator with pedestal junction
Seal.
Beneficial effect：The tissue culture effect of aerosol tissue culture device of the present utility model is good, and Liquid Culture is using shake
Swing or shaking table waggle formula can all produce shearing force, be unfavorable for the growth of culture, and dissolved oxygen content is very in nutrient solution
Low, for aerosol tissue cultures without shearing force, dissolved oxygen is more in droplet, is advantageous to the growth and development of culture, aerosol by contrast
Liquid and gas contradiction has been coordinated in culture, enhances gas exchanges, and the plant of cultivation is more healthy and stronger, and breeding potential is high, in incubation
Carbon Dioxide in Air is passed through, carbonless base paper can be carried out, is advantageous to plant and external environment, bottle is faster adapted to during domestication
Interior humidity is not up to 100% humidity in solid culture and the such bottle of Liquid Culture, and plant stomata function is more sound, reduces glass
Change plant, and use aerosol culture, without agar or gel, save cost.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model；
Fig. 2 is sectional view of the present utility model；
Fig. 3 is three-dimensional cutaway view of the present utility model；
Fig. 4 is that the utility model gas filtration threading leads directly to component diagram：
Fig. 5 is the utility model mozzle schematic diagram.
Embodiment
Technical solutions of the utility model are described in detail below, but the scope of protection of the utility model is not limited to
The embodiment.
As shown in Figure 1 to Figure 3, a kind of aerosol tissue culture device of the present utility model, including bottle cap 1, bottle 2, carrying
Net 3, mozzle 4, supersonic generator 5 and pedestal 6, bottle cap 1 and bottle 2 form aerosol receiving space, and bottle cap 1 is by high temperature resistant PC
Material is made, volume 2.0L, can directly carry out autoclave sterilization with high pressure resistant after sealing, bottle cap 1 is provided with gas filtration
Its inner space is divided into two parts inside the bottle 2 by device, bearer network 3, and bearer network 3 is PC plastic disk, φ 115mm,
The top of bottle 2 is culture vitellarium, lower storage nutrient solution, and mozzle 4 is fixed on the bottom of bottle 2 through bearer network 3, and it is pushed up
Portion is 3cm away from bottle cap 1, is fixed on ultrasonic wave caused by the supersonic generator 5 of pedestal 6 by the nutrient liquid atomizing in bottle 2 simultaneously
Discharged and spread by mozzle 4, include the droplet of mixed gas and nutrient solution, overflowed, declined to surrounding by mozzle, be
Culture provides nutritional ingredient and dissolved oxygen, and aerosol culture apparatus also includes control system, and control system is to control ultrasonic wave
Device 5 starts the timer of time, and gas-filtering device includes filter membrane and gas filtration threading leads directly to component, and filter membrane is located at bottle cap 1
Center through hole 7 at, preferably, central air vents are φ 10mm, 2.2um filter membranes are welded with, as shown in figure 4, gas filtration
Threading leads directly to component at the perforation 8 of the periphery of center through hole 7, and gas filtration threading, which leads directly to component, includes screw thread threading straight-through 9,
The first silica gel pad 10, the second silica gel pad 11 and locking nut 12, screw thread threading for being arranged in screw thread threading straight-through 9 successively are straight
The silicone tube 18 and the online biofilters 19 of 2.2um that logical 9 couplings are sequentially connected, the first silica gel pad 10, the second silica gel
Gas filtration threading is led directly to component by locking nut 12 and is fixed on bottle cap 1, bottle cap 1 is at least by pad 11 located at 8 both sides of perforation
It is equipped with more than two gas filtration threadings and leads directly to component, the side wall of bottle 2 is provided with kink 13 in the diagonal and vertically side
To contraction flow region is extended, the bearer network 3 in bottle 2 is snapped in kink 13, as shown in figure 5, the top of mozzle 4 is provided with envelope
Lid, the bottom of mozzle 4 are provided with fence, and the side wall of mozzle 4 is offered between top and bearer network 3 for droplet diffusion
Opening 14, the bottom of bottle 2 are provided with the annular groove 15 of depression, and the bottom of mozzle 4 is fixed in annular groove 15, and pedestal 6 also includes super
Sound wave fixed mount 16 and ultrasonic piece fastener 17, preferably, being located at from 35W ultrasonic wave generating sheet supersonic generator 5
On ultrasonic wave fixed mount 16, and pedestal 6 is fixed on by ultrasonic piece fastener 17, bottle cap 1 and bottle 2, bottle 2 and pedestal 6 with
And supersonic generator 5 is equipped with sealing ring with the junction of pedestal 6, prevents nutrient solution droplet from evaporateing into outside device.
As described above, although the utility model has been represented and described with reference to specific preferred embodiment, but it must not
It is construed to the limitation to the utility model itself.In the spirit and scope of the present utility model for not departing from appended claims and defining
Under the premise of, can various changes can be made in the form and details to it.
Claims (10)
- A kind of 1. aerosol tissue culture device, it is characterised in that：Including bottle cap (1), bottle (2), bearer network (3), mozzle (4), supersonic generator (5) and pedestal (6), the bottle cap (1) and bottle (2) form aerosol receiving space, the bottle cap (1) Provided with gas-filtering device, its inner space is divided into two parts, the water conservancy diversion by the bearer network (3) located at bottle (2) inside Pipe (4) is fixed on bottle (2) bottom through bearer network (3), is fixed on the supersonic generator (5) of pedestal (6) by bottle (2) Nutrient liquid atomizing and pass through mozzle (4) discharge diffusion.
- 2. aerosol tissue culture device according to claim 1, it is characterised in that：The gas-filtering device includes filter membrane Component is led directly to gas filtration threading, the filter membrane is located at center through hole (7) place of bottle cap (1), and the gas filtration threading is straight Logical component is located at perforation (8) place of center through hole (7) periphery.
- 3. aerosol tissue culture device according to claim 2, it is characterised in that：The gas filtration threading leads directly to component (9) are led directly to including screw thread threading, are arranged in the first silica gel pad (10), the second silica gel pad that screw thread threading leads directly to (9) successively (11) and locking nut (12), screw thread threading lead directly to silicone tube (18) and the online bacteriological filter that (9) coupling is sequentially connected Device (19), the first silica gel pad (10), the second silica gel pad (11) are located at perforation (8) both sides, by locking nut (12) by gas Body filtering threading leads directly to component and is fixed on bottle cap (1).
- 4. aerosol tissue culture device according to claim 3, it is characterised in that：The bottle cap (1) is at least equipped with two Gas filtration threading more than individual leads directly to component.
- 5. aerosol tissue culture device according to claim 1, it is characterised in that：Bottle (2) side wall is provided with oblique Interior kink (13) simultaneously in axial direction extends contraction flow region, and the bearer network (3) in the bottle (2) is snapped in kink (13)。
- 6. aerosol tissue culture device according to claim 1, it is characterised in that：The top of the mozzle (4) is provided with Capping, the bottom of mozzle (4) are provided with fence, and mozzle (4) side wall, which is located between top and bearer network (3), offers confession The opening (14) of droplet diffusion.
- 7. the aerosol tissue culture device according to claim 1 or 6, it is characterised in that：Bottle (2) bottom is provided with recessed Sunken annular groove (15), mozzle (4) bottom are fixed in annular groove (15).
- 8. aerosol tissue culture device according to claim 1, it is characterised in that：The pedestal (6) also includes ultrasonic wave Fixed mount (16) and ultrasonic piece fastener (17), the supersonic generator (5) pass through on ultrasonic wave fixed mount (16) Ultrasonic piece fastener (17) is fixed on pedestal (6).
- 9. aerosol tissue culture device according to claim 1, it is characterised in that：Also include control system, the control System starts the timer of time for control supersonic generator (5).
- 10. according to the aerosol tissue culture device any one of claim 1,4,5,6,8,9, it is characterised in that：It is described Bottle cap (1) and bottle (2), bottle (2) are equipped with sealing with pedestal (6) and supersonic generator (5) with pedestal (6) junction Circle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720499327.7U CN207002738U (en) | 2017-05-08 | 2017-05-08 | A kind of aerosol tissue culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720499327.7U CN207002738U (en) | 2017-05-08 | 2017-05-08 | A kind of aerosol tissue culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207002738U true CN207002738U (en) | 2018-02-13 |
Family
ID=61442567
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720499327.7U Expired - Fee Related CN207002738U (en) | 2017-05-08 | 2017-05-08 | A kind of aerosol tissue culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207002738U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184674A (en) * | 2018-03-14 | 2018-06-22 | 上海电机学院 | A kind of ultrasonic wave device for cultivating plant tissue |
-
2017
- 2017-05-08 CN CN201720499327.7U patent/CN207002738U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184674A (en) * | 2018-03-14 | 2018-06-22 | 上海电机学院 | A kind of ultrasonic wave device for cultivating plant tissue |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203048953U (en) | Dismountable plant tissue fluid culture device | |
| CN207002738U (en) | A kind of aerosol tissue culture device | |
| CN106906139A (en) | A kind of aerosol tissue culture device | |
| CN109072151A (en) | The fixation fixture of cell culture container and cell culture container | |
| CN103031251A (en) | Multifunctional plant excised root culture bioreactor system | |
| CN203999609U (en) | Anerobe dialysis culturing device | |
| CN206665232U (en) | The packing container of matrix used in a kind of muscardine production | |
| CN205115494U (en) | Cell culture bottle | |
| CN211311449U (en) | Silage maize fermenting installation | |
| CN108450243A (en) | A kind of fermentation box of fungi stationary culture | |
| CN105210866B (en) | A kind of intermittent immersed orchid protocorms propagation quick-breeding method | |
| CN108464239A (en) | A kind of plant tissue culture container | |
| CN212077079U (en) | Cell culture bottle | |
| CN201358254Y (en) | Novel biofilm reactor | |
| CN206266628U (en) | Culture device for liquid strain of edible fungus | |
| CN209162085U (en) | A kind of fermentor of animal cell culture | |
| CN112094733A (en) | A aseptic closing device that breathes freely of portable for laboratory tissue culture container | |
| CN206887113U (en) | A kind of classification inoculation apparatus of liquid spawn fermentation tank | |
| CN208354275U (en) | A kind of plant tissue culture vessel | |
| CN204860463U (en) | Can be used to add in succession tissue culture bottle of nutrient solution | |
| CN2107468U (en) | Small liquid deep layer ventilation culture device | |
| CN217662322U (en) | Air supply filter device for haematococcus pluvialis cultivation | |
| CN218932161U (en) | Plant biological fermentation tank convenient for in-tank reaction observation | |
| CN214244420U (en) | Simple industrial microorganism activation culture device and system | |
| CN206089638U (en) | Anaerobe fermenting installation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180213 Termination date: 20210508 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |