CN205284511U - Plant tissue culture device - Google Patents
Plant tissue culture device Download PDFInfo
- Publication number
- CN205284511U CN205284511U CN201620075223.9U CN201620075223U CN205284511U CN 205284511 U CN205284511 U CN 205284511U CN 201620075223 U CN201620075223 U CN 201620075223U CN 205284511 U CN205284511 U CN 205284511U
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- China
- Prior art keywords
- bottle
- plant tissue
- plug
- utility
- model
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- Expired - Fee Related
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Abstract
The utility model discloses a plant tissue culture device, including blake bottle, cultivation bottle plug, air duct, the stopcock of ventilating, breather pipe and cotton plug, train the bottle plug set up in the opening part of blake bottle, the stopcock of ventilating set up in the one end of breather pipe, the air duct is connected train the bottle plug with ventilate between the stopcock, the cotton plug set up in the other end of breather pipe. Compared with the prior art, the utility model discloses there are three characteristics: firstly, the blake bottle and train the bottle plug between seal with paraffin, can prevent the air in the microorganism fall into in the blake bottle. The air duct prevents the material that little biological pollution cultivateed, the air permeability that also enables the blake bottle strengthens, satisfies the needs of the respiration of culture better. Thirdly, breather pipe mouth down, the microorganism will reach the effect that the culture medium just can not rely on self gravity like this, and only be chemotactic effect, has reduced the possibility that the culture medium received little biological pollution.
Description
Technical field
The utility model relates to a kind of experimental installation, particularly relates to a kind of device for cultivating plant tissue.
Background technology
Plant tissue culture bottle general in laboratory is the big mouth culturing bottle with plastic cover or the Erlenmeyer flask of band tampon, these culturing bottles due to bottleneck upward, the effect of microorganism in air due to self gravitation and the chemotaxis to sugar are easy to drop on cultivation bottleneck and arrive substratum along culturing bottle wall, are easy to polluting material.
Practical novel content
The purpose of this utility model is just to provide a kind of device for cultivating plant tissue to solve the problem.
The utility model is achieved through the following technical solutions above-mentioned purpose:
The utility model comprises culturing bottle, cultivates bottle stopper, airway, ventilation pipe close, ventpipe and cotton plug, described cultivation bottle stopper is arranged at the opening place of described culturing bottle, described ventilation pipe close is arranged at one end of described ventpipe, described airway is connected between described cultivation bottle stopper and described ventilation pipe close, and described cotton plug is arranged at the other end of described ventpipe.
Specifically, described culturing bottle is triangular flask, adopts paraffin sealing between described culturing bottle and described cultivation bottle stopper; The diameter of described airway is 6mm, and length is 20cm, and the diameter of described ventpipe is 2cm, is highly 6cm; Described airway is M type.
The beneficial effects of the utility model are:
The utility model is a kind of device for cultivating plant tissue, compared with prior art, the utility model has three features: one is culturing bottle and cultivates between bottle stopper with paraffin sealing, makes them become an airtight entirety, it is possible to prevent microbes in air from falling in culturing bottle. Two is have bending airway (a diameter 6mm copper pipe) compact siro spinning technology to cultivate bottle stopper (rubber plug) and ventilation pipe close (rubber plug), the length of airway is about about 20cm, it is folded into four parts, upper end exceeds cultivates bottle stopper mouth, and lower end and media surface exceed media surface mutually together or a little. Airway long like this is exactly that the microorganism allowing and having passed through tampon is difficult to reach substratum within short duration, thus prevents the material that microbial contamination is cultivated; Also the air permeability of culturing bottle can be made to strengthen, meet the needs of the respiration of culture better. Three is ventpipe mouth down, and such microorganism to be reached the effect that substratum just can not rely on self gravitation, and is only chemotactic effect, decreases the possibility of substratum by microbial contamination. Therefore, this kind of culturing bottle can be placed in open environment (as: illumination box), and do not need to be placed in special plant tissue culture room and carry out tissue culture.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
In figure: 1-culturing bottle, 2-cultivate bottle stopper, 3-airway, 4-ventilate pipe close, 5-ventpipe, 6-cotton plug.
Embodiment
Below in conjunction with accompanying drawing, the utility model is described in further detail:
As shown in Figure 1: the utility model comprises culturing bottle 1, cultivation bottle stopper 2, airway 3, ventilation pipe close 4, ventpipe 5 and cotton plug 6, described cultivation bottle stopper 2 is arranged at the opening place of described culturing bottle 1, described ventilation pipe close 4 is arranged at one end of described ventpipe 5, described airway 3 is connected between described cultivation bottle stopper 2 and described ventilation pipe close 4, and described cotton plug 6 is arranged at the other end of described ventpipe 5.
Specifically, described culturing bottle is triangular flask, adopts paraffin sealing between described culturing bottle and described cultivation bottle stopper; The diameter of described airway 3 is 6mm, and length is 20cm, and the diameter of described ventpipe 5 is 2cm, is highly 6cm; Described airway 3 is M type.
Embodiment:
1, stamp the hole of diameter 5mm in the central authorities cultivating bottle stopper 2 and ventilation pipe close 4 with punch tool.
2, with the copper pipe of length to be 20cm diameter be 6mm (internal diameter 4mm) as airway 3, bend to M pattern shape, it is inserted into and cultivates in bottle stopper 2 and ventilation pipe close 4.
3, the soft rubber ball (cultivating bottle stopper 2) of airway 3 one end is inserted into the bottleneck of the Erlenmeyer flask (culturing bottle 1) that volume is 100mL, the soft rubber ball (ventilation pipe close 4) of one end is inserted into the upper end open of ventpipe 5 in addition, cotton plug 6 on the lower end plug of ventpipe 5, for gaseous interchange. So far, a plant tissue culture bottle completes.
4, for plant tissue culture, time, first take out and cultivate bottle stopper 2, in culturing bottle 1, then add the plant tissue culture media of 50mL, cover and cultivate bottle stopper 2.
5, being wrapped by plant tissue culture bottle 1 with old newsprint, put into high-pressure sterilizing pot, 121 DEG C of sterilizing 30min, transfer in Bechtop after cooling. After culture medium solidifying, plant tissue is inoculated in substratum. Each interface is closed with the whiteruss dissolved.
6, culturing bottle 1 is transferred in incubator cultivate.
More than show and describe ultimate principle of the present utility model and main feature and advantage of the present utility model. The technician of the industry should understand; the utility model is not restricted to the described embodiments; what describe in above-described embodiment and specification sheets just illustrates principle of the present utility model; under the prerequisite not departing from the utility model spirit and scope; the utility model also has various changes and modifications, and these changes and improvements all fall within the scope of claimed the utility model. The claimed scope of the utility model is defined by appending claims and equivalent thereof.
Claims (5)
1. a device for cultivating plant tissue, it is characterized in that: comprise culturing bottle, cultivate bottle stopper, airway, ventilation pipe close, ventpipe and cotton plug, described cultivation bottle stopper is arranged at the opening place of described culturing bottle, described ventilation pipe close is arranged at one end of described ventpipe, described airway is connected between described cultivation bottle stopper and described ventilation pipe close, and described cotton plug is arranged at the other end of described ventpipe.
2. device for cultivating plant tissue according to claim 1, it is characterised in that: described culturing bottle is triangular flask.
3. device for cultivating plant tissue according to claim 1, it is characterised in that: adopt paraffin sealing between described culturing bottle and described cultivation bottle stopper.
4. device for cultivating plant tissue according to claim 1, it is characterised in that: the diameter of described airway is 6mm, and length is 20cm, and the diameter of described ventpipe is 2cm, is highly 6cm.
5. device for cultivating plant tissue according to claim 1, it is characterised in that: described airway is M type.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620075223.9U CN205284511U (en) | 2016-01-26 | 2016-01-26 | Plant tissue culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620075223.9U CN205284511U (en) | 2016-01-26 | 2016-01-26 | Plant tissue culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205284511U true CN205284511U (en) | 2016-06-08 |
Family
ID=56427334
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620075223.9U Expired - Fee Related CN205284511U (en) | 2016-01-26 | 2016-01-26 | Plant tissue culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205284511U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111345235A (en) * | 2020-04-22 | 2020-06-30 | 河南科技大学 | Type of ventilating group banks up bottle with earth |
-
2016
- 2016-01-26 CN CN201620075223.9U patent/CN205284511U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111345235A (en) * | 2020-04-22 | 2020-06-30 | 河南科技大学 | Type of ventilating group banks up bottle with earth |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160608 Termination date: 20170126 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |