CN208201004U - A kind of device for the culture plant cell that suspends - Google Patents
A kind of device for the culture plant cell that suspends Download PDFInfo
- Publication number
- CN208201004U CN208201004U CN201820449228.2U CN201820449228U CN208201004U CN 208201004 U CN208201004 U CN 208201004U CN 201820449228 U CN201820449228 U CN 201820449228U CN 208201004 U CN208201004 U CN 208201004U
- Authority
- CN
- China
- Prior art keywords
- upper half
- top cover
- lower half
- suspends
- drainage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000003756 stirring Methods 0.000 claims abstract description 19
- 238000012360 testing method Methods 0.000 claims description 4
- 238000004891 communication Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 description 6
- 238000004140 cleaning Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000001464 adherent effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a kind of device of culture plant cell that suspends, including bottle body, top cover, stirring ball；The bottle body includes the upper half and lower half；The interior of the upper half is provided with internal screw thread, and the outer wall of the lower half is provided with the external screw thread with screw-internal thread fit, the upper half and lower half by being threadedly engaged flexible connection；It is provided with top opening at the top of the upper half, side is provided with setting-out mouth and sample tap；Top cover is provided in the top opening；The bottom of the lower half is provided with convex drainage platform, and the side wall of the drainage platform is the arc of indent, forms an arc drainage trough with the side wall of lower half；The bottom of the top cover is provided with connecting line stirring ball connected to it；The inner sidewall of the upper half is provided with the spiral drainage strip of arrangement clockwise from up to down, and the section of the spiral drainage strip is hemispherical dome structure.
Description
Technical field
The utility model relates to field of biotechnology, and in particular to a kind of device for the culture plant cell that suspends.
Background technique
The culture that suspends is a kind of common cell culture technology, is a kind of in the Liquid Culture for constantly being stirred or being shaken
In base, unicellular and small cell cluster technology is cultivated, while being also a kind of training method of non-adherent dependent cell.Certain patches
Wall dependent cell can also use the method culture by adapting to and selecting.There is all kinds of suspension cell culture dresses in the prior art
It sets, is mostly to be stirred with built-in stirring rod cooperation magnetic stirring apparatus, has certain mixing effect, but the fortune of culture solution
It moves based on horizontal rotation, when revolving speed is lower, the effect of upper and lower level culture solution fast exchange is not achieved；When revolving speed is higher, though
Convection current whirlpool can be so formed, realizes the exchange of upper and lower level culture solution, but faster flow velocity can generate wound to fragile cell
Evil；In addition, there is also rotors arbitrarily to replace for suspension cell culture device in the prior art, device cleaning inside is difficult
The problems such as.
Utility model content
The utility model in order to make up for the deficiencies of the prior art, provides a kind of device of culture plant cell that suspends.
What the utility model was achieved through the following technical solutions:
A kind of device for the culture plant cell that suspends, including bottle body, top cover, stirring ball；The bottle body include the upper half and
Lower half；The interior of the upper half is provided with internal screw thread, and the outer wall of the lower half is provided with and screw-internal thread fit
External screw thread, the upper half and lower half are by being threadedly engaged flexible connection；It is provided with top opening at the top of the upper half, side is set
It is equipped with setting-out mouth and sample tap；Top cover is provided in the top opening；The bottom of the lower half is provided with convex drainage
The side wall of platform, the drainage platform is the arc of indent, forms an arc drainage trough with the side wall of lower half；The top
The bottom of lid is provided with connecting line stirring ball connected to it；The inner sidewall of the upper half is provided with up time from up to down
The spiral drainage strip of needle direction arrangement, the section of the spiral drainage strip are hemispherical dome structure.
Preferably, air inlet pipe, the air inlet pipe test are provided on the side wall of the upper half, upper end is in communication with the outside,
It is provided with valve, multiple ventholes are provided on the side wall of the air inlet pipe；It is provided on the top cover and has valve
Exhaust pipe.
Preferably, the bottom of the top cover is provided with fixed ring, and the connecting line is installed in fixed ring.
Preferably, multiple pits are provided on the outer wall of the stirring ball.
It suspends in the present invention and cultivates the device of plant cell, bottle body is divided into upper and lower two parts being flexibly connected first, clearly
When clean, the two is dismantled and is separately handled, internal cleaning can be realized without using this kind of burnisher of test tube brush, it can
Scuffing caused by when effectively avoiding burnisher the cleaning of bottle body inner wall, effectively improves service life, while also reducing
Time needed for cleaning；Secondly, dismountable design, allows to arbitrarily have difference according to the diameter of stirring ball to replace
The lower half of size drainage trough, improves versatility, and the best match of stirring ball and drainage trough size may be implemented, and improves stirring
Effect；By the way that spiral drainage strip is arranged on bottle body inner wall, the culture solution in rotation can be drained downwards on inner wall, together
When drainage platform at drain upwards, thus inside culture apparatus formed a flow circuit, greatly improve drainage effect
Rate, can be realized the uniform stirring of culture solution under lower revolving speed, effectively prevent the damage in whipping process to plant cell
Wound；By the way that air inlet pipe and exhaust pipe is further arranged, the gas of different component can be supplemented into culture solution, or pressed
Power is adjusted；Multiple ventholes are set on air inlet tube wall, diffuser efficiency of the gas in culture solution can be improved；It is fixed by setting
Ring can replace stirring ball under the premise of being changed without top cover, improve ease of use；By further being set in stirring ball surface
Pit is set, stirring ball can be made to form turbulent flow while agitating, disturb liquid internal, improve mixed effect.
Detailed description of the invention
Fig. 1 is to suspend to cultivate the structure chart of the device of plant cell in the utility model；
Fig. 2 is to suspend to cultivate the cross-sectional view of the device of plant cell in the utility model.
Specific embodiment
The technical solution of the utility model is described in detail below by specific embodiment, but this is practical new
The range of type is not restricted by the embodiments.
Such as Fig. 1 and 2, a kind of device of culture plant cell that suspends, including bottle body, top cover 2, stirring are present embodiments provided
Ball 3；The bottle body includes the upper half 11 and lower half 12；The interior of the upper half 11 is provided with internal screw thread, under described
The outer wall of half portion 12 is provided with the external screw thread with screw-internal thread fit, the upper half 11 and lower half 12 by being threadedly engaged activity even
It connects；Top opening 110 is provided at the top of the upper half 11, side is provided with setting-out mouth 111 and sample tap 112；The top
Top cover 2 is provided in opening 110；The bottom of the lower half 12 is provided with convex drainage platform 121, the drainage platform
121 side wall is the arc of indent, cooperatively forms an arc drainage trough with the bottom sidewall of lower half 12；The top cover 2
Bottom be provided with the stirring ball 3 connected to it of connecting line 22；The inner sidewall of the upper half 11 is provided with from up to down
The spiral drainage strip 113 of arrangement clockwise, the section of the spiral drainage strip are hemispherical dome structure；The upper half 11
Air inlet pipe 114,114 test of air inlet pipe are provided on side wall, upper end is in communication with the outside, and is provided with valve, it is described into
Multiple ventholes are provided on the side wall of tracheae 114；It is provided on the top cover 2 and with valvular exhaust pipe 23；The top
The bottom of lid 2 is provided with fixed ring 21, and the connecting line 22 is installed in fixed ring 21；It is arranged on the outer wall of the stirring ball 2
There are multiple pits.
Above embodiment is only the preferred embodiment of the utility model, it should be pointed out that, for ordinary skill
For people, what is carried out under the premise of not departing from the utility model is partly improved, still in the protection scope of the utility model it
In.
Claims (4)
1. a kind of device for the culture plant cell that suspends, including bottle body, top cover, stirring ball；The bottle body includes the upper half under
Half portion；The interior of the upper half is provided with internal screw thread, and the outer wall of the lower half is provided with outer with screw-internal thread fit
Screw thread, the upper half and lower half are by being threadedly engaged flexible connection；Top opening, side setting are provided at the top of the upper half
There are setting-out mouth and sample tap；Top cover is provided in the top opening；It is flat that the bottom of the lower half is provided with convex drainage
The side wall of platform, the drainage platform is the arc of indent, forms an arc drainage trough with the side wall of lower half；The top cover
Bottom be provided with connecting line stirring ball connected to it；The inner sidewall of the upper half is provided with clockwise from up to down
The spiral drainage strip of direction arrangement, the section of the spiral drainage strip are hemispherical dome structure.
2. the device of the culture plant cell according to claim 1 that suspends, it is characterised in that: on the side wall of the upper half
It is provided with air inlet pipe, the air inlet pipe test, upper end is in communication with the outside, and valve is provided with, on the side wall of the air inlet pipe
It is provided with multiple ventholes；It is provided on the top cover and with valvular exhaust pipe.
3. the device of the culture plant cell according to claim 2 that suspends, it is characterised in that: the bottom of the top cover is arranged
There is fixed ring, the connecting line is installed in fixed ring.
4. the device of the culture plant cell according to claim 3 that suspends, it is characterised in that: on the outer wall of the stirring ball
It is provided with multiple pits.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820449228.2U CN208201004U (en) | 2018-04-02 | 2018-04-02 | A kind of device for the culture plant cell that suspends |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820449228.2U CN208201004U (en) | 2018-04-02 | 2018-04-02 | A kind of device for the culture plant cell that suspends |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208201004U true CN208201004U (en) | 2018-12-07 |
Family
ID=64525934
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820449228.2U Expired - Fee Related CN208201004U (en) | 2018-04-02 | 2018-04-02 | A kind of device for the culture plant cell that suspends |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208201004U (en) |
-
2018
- 2018-04-02 CN CN201820449228.2U patent/CN208201004U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181207 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |