CN206843498U - Living cells rotating separation device - Google Patents
Living cells rotating separation device Download PDFInfo
- Publication number
- CN206843498U CN206843498U CN201720317706.XU CN201720317706U CN206843498U CN 206843498 U CN206843498 U CN 206843498U CN 201720317706 U CN201720317706 U CN 201720317706U CN 206843498 U CN206843498 U CN 206843498U
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- China
- Prior art keywords
- separator
- suction pipe
- living cells
- culture tank
- liquid suction
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Abstract
It the utility model is related to a kind of living cells rotating separation device, including motor, rotating shaft, separator and liquid suction pipe, separator is to be arranged at the hollow sphere inside culture tank, hole is circumferentially provided with the ball wall of separator radial level, the bottom of separator is provided with inlet, the lower end of separator and rotating shaft, which is fixed and driven by motor, to rotate, and the lower end of liquid suction pipe extend into separator interior, upper end passes at the top of culture tank, and the drawing liquid import of liquid suction pipe is arranged at the nearly sphere center position of separator.The utility model can be efficiently separated to culture cell inside culture tank and replace culture waste liquid, avoid nutrient solution from being contaminated.
Description
Technical field
The utility model belongs to the technical field of cell culture separation, is filled more particularly to a kind of living cells rotating separation
Put.
Background technology
During tank body culture is carried out to cell, with the growth of cell, the nutriment in nutrient solution can be gradual
It is consumed so as to produce the nutrient solution of low nutrition concentration or waste liquid, and contains cell metabolite, is unfavorable for the life of living cells
It is long, it is necessary to the culture waste liquid in tank body is extracted out and supplements fresh nutrient solution, need in this process in nutrient solution
Living cells carries out separation reservation.
Traditional operating method is that the nutrient solution in tank body is drawn to outside tank to carry out separating treatment, not only troublesome in poeration,
The complexity of equipment is added, and contaminated risk in separation process be present.
Utility model content
Technical problem to be solved in the utility model is to provide a kind of living cells rotating separation device, can be in culture tank
It is internal that culture cell is efficiently separated and replaces culture waste liquid, avoid polluting.
Technical scheme is to provide a kind of living cells rotating separation device used by the utility model solves its technical problem,
Including motor, rotating shaft, separator and liquid suction pipe, the separator is to be arranged at the hollow sphere inside culture tank, separator footpath
Hole is circumferentially provided with to the ball wall of level, and the bottom of separator is provided with inlet, and the lower end of the separator and rotating shaft is consolidated
Fixed and driven and rotate by motor, the lower end of the liquid suction pipe extend into separator interior, upper end passes at the top of culture tank, institute
The drawing liquid import for stating liquid suction pipe is arranged at the nearly sphere center position of separator.
As a kind of preferred embodiment of the utility model, the motor is installed on the top of culture tank, the rotating shaft
It is rotatable sealing structure between culture tank lid.
As the utility model another preferred embodiment, the lower end of the liquid suction pipe is extend into point by inlet
From in device.
It is sealing knot between the liquid suction pipe and culture tank lid as the utility model another preferred embodiment
Structure.
As the utility model another preferred embodiment, the upper port of the liquid suction pipe is provided with closure.
Beneficial effect
In the utility model, spherical separator is arranged in the nutrient solution in culture tank, when motor driven separator revolves
When turning, the liquid in separator also rotates with, and when being rotated up to certain speed, the living cells in nutrient solution is due to quality
Compared with nutrient solution weight, caused centrifugal force is bigger, and living cells tends to the edge of the horizontal diameter of separator spheroid, by hole stream
Going out, the light liquid without living cells tends to the axis of rotation of separator, and light liquid is extracted out at the nearly centre of sphere by liquid suction pipe,
The waste to living cells is farthest reduced, while the nutrient solution in culture tank is inhaled into separator from inlet and carried out
Constantly separation.Therefore, the utility model can efficiently be separated inside culture tank to culture cell, and extract culture out
Waste liquid avoids nutrient solution and is contaminated in separation process to replace fresh nutrient solution.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the structural representation that the utility model is arranged in culture tank.
Embodiment
With reference to specific embodiment, the utility model is expanded on further.It should be understood that these embodiments are merely to illustrate this
Utility model rather than limitation the scope of the utility model.In addition, it is to be understood that reading the content of the utility model instruction
Afterwards, those skilled in the art can make various changes or modifications to the utility model, and these equivalent form of values equally fall within this Shen
Please appended claims limited range.
A kind of living cells rotating separation device as depicted in figs. 1 and 2, including motor 1, rotating shaft 2, separator 3 and drawing liquid
Pipe 4.
Motor 1 is arranged on the top of culture tank 6, and the upper end of rotating shaft 2 is by the output axis connection of shaft coupling and motor 1 and passes through
The driving rotation of motor 1.Rotating shaft 2 is extend into inside culture tank 6 through the top cover of culture tank 6, between rotating shaft 2 and the lid of culture tank 6
For rotatable sealing structure, prevent microorganism is entered in culture tank 6 from polluting.
Separator 3 is arranged inside culture tank 6, is hollow-sphere structures, on the ball wall of the radial level of separator 3 circumferentially
It is provided with hole 9, the bottom of separator 3 is provided with inlet 5, and the lower end of separator 3 and rotating shaft 2, which is fixed and driven by motor 1, revolves
Turn.The lower end of liquid suction pipe 4 is extend into inside separator 3 by inlet 5, and the drawing liquid import 7 of liquid suction pipe 4 is arranged at separator 3
Nearly sphere center position, the upper end of liquid suction pipe 4 passes from the top of culture tank 6, is sealing structure between the lid of culture tank 6, prevents
Microorganism pollution.Upper port forms negative pressure during 4 drawing liquid of liquid suction pipe, and upper port is provided with closure, to the liquid suction pipe 4 of non-drawing liquid state
Sealed, prevent microorganism is entered in culture tank 6 from liquid suction pipe 4 from being polluted to cell culture.
The living cells rotating separation device is arranged in the nutrient solution in culture tank 6, when motor 1 rotates with flow separator 3
When, the liquid in separator 3 also rotates with, and when being rotated up to certain speed, the living cells in nutrient solution is due to quality
Compared with nutrient solution weight, caused centrifugal force is bigger, and living cells tends to the edge of the horizontal diameter of the spheroid of separator 3, is flowed by hole 4
Go out, light liquid without living cells tends to the axis of rotation of separator 3, and obtains at the centre of sphere of separator 3 being substantially free of work
The light liquid of cell, liquid suction pipe 4 are extracted out to light liquid at the nearly centre of sphere of separator 3, farthest reduced to living cells
Waste.While carrying out cell separation, light liquid is extracted out, the nutrient solution in culture tank 6 is inhaled into separator 3 from inlet 5
It is middle constantly to be separated.The top cover of culture tank 6 is provided with fluid infusion 8, and fresh cultured is filled into culture tank 6 by fluid infusion 8
Liquid, reach the continuous effect extracted waste liquid out, fill into fresh liquid, realize the displacement of nutrient solution, and pollution risk is not present.
Claims (5)
1. a kind of living cells rotating separation device, including motor (1), rotating shaft (2), separator (3) and liquid suction pipe (4), its feature
It is：The separator (3) is to be arranged at the internal hollow sphere of culture tank (6), edge on the ball wall of separator (3) radial level
Hole (9) circumferentially is provided with, the bottom of separator (3) is provided with inlet (5), and the separator (3) and the lower end of rotating shaft (2) are fixed
And driven and rotated by motor (1), the lower end of the liquid suction pipe (4) extend into that separator (3) is internal, upper end is from culture tank (6)
Top passes, and the drawing liquid import (7) of the liquid suction pipe (4) is arranged at the nearly sphere center position of separator (3).
A kind of 2. living cells rotating separation device according to claim 1, it is characterised in that：The motor (1) is installed on
The top of culture tank (6), it is rotatable sealing structure between the rotating shaft (2) and culture tank (6) lid.
A kind of 3. living cells rotating separation device according to claim 1, it is characterised in that：Under the liquid suction pipe (4)
End is extend into separator (3) by inlet (5).
A kind of 4. living cells rotating separation device according to claim 1, it is characterised in that：The liquid suction pipe (4) and training
It is sealing structure between foster tank (6) lid.
A kind of 5. living cells rotating separation device according to claim 1, it is characterised in that：The liquid suction pipe (4) it is upper
Port is provided with closure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720317706.XU CN206843498U (en) | 2017-03-29 | 2017-03-29 | Living cells rotating separation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720317706.XU CN206843498U (en) | 2017-03-29 | 2017-03-29 | Living cells rotating separation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206843498U true CN206843498U (en) | 2018-01-05 |
Family
ID=60791889
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720317706.XU Active CN206843498U (en) | 2017-03-29 | 2017-03-29 | Living cells rotating separation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206843498U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108148736A (en) * | 2018-01-12 | 2018-06-12 | 上海元创生物科技有限公司 | Living cells clear liquid separator built in bioreactor |
-
2017
- 2017-03-29 CN CN201720317706.XU patent/CN206843498U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108148736A (en) * | 2018-01-12 | 2018-06-12 | 上海元创生物科技有限公司 | Living cells clear liquid separator built in bioreactor |
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