CN207418765U - Portable suspension cell culture and chemicals dosing plant - Google Patents
Portable suspension cell culture and chemicals dosing plant Download PDFInfo
- Publication number
- CN207418765U CN207418765U CN201721476349.8U CN201721476349U CN207418765U CN 207418765 U CN207418765 U CN 207418765U CN 201721476349 U CN201721476349 U CN 201721476349U CN 207418765 U CN207418765 U CN 207418765U
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- CN
- China
- Prior art keywords
- suspension cell
- connecting tube
- blake bottle
- dosing
- cell culture
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
The utility model discloses a kind of portable suspension cell culture and chemicals dosing plant, including sharp bottom blake bottle, Y types connecting tube and doser；Sharp bottom blake bottle is equipped with sharp bottom and cultivates bottle cap, and sharp bottom culture bottle cap is equipped with elastic aperture；Sharp bottom blake bottle is installed on constant level's shaking table；The two entrances of Y type connecting tubes connect two dosers by latex connecting tube respectively；Latex connecting tube is equipped with spring clip；The outlet of Y type connecting tubes is connected with a dosing conduit；Dosing conduit connects a dosing catheter needle；Dosing catheter needle passes through elastic aperture to be connected with sharp bottom blake bottle.The utility model can realize the inoculation carried out to suspension cell under gnotobasis effective and safe, and normal sterile culture is carried out to suspension cell, can also apply one or two kinds of different type drug to suspension cell；Above-mentioned all basic processes are easy to operate and without unscrewing culture bottle cap, are conducive to appropriate culture conventional to suspension cell.
Description
Technical field
The utility model is related to cell culture apparatus technical fields, and in particular to a kind of portable suspension cell culture and plus
Medicine device.
Background technology
Cell culture is a kind of common cytologic experiment, Physiology Experiment, toxicological experiment, pharmacological experiment, drug effect
Learn the conventional method of the related experiments such as experiment.In general, suspension cell culture needs cell to be in suspended state and needs nothing
Bacterium, nontoxic Essential Environment are cultivated this requires the suspension cell to required culture carries out sterile isolation, environment are avoided with this
In various microorganisms enter in plant cell suspension cultures, while be smoothed out for all kinds of infrastests, it is also necessary to right
The suspension cell cultivated under the conditions of sterile, nontoxic carries out a kind of drug or two kinds of different type drug dosings, this is just suspension
Specification, material, auxiliary device of Tissue Culture Flask etc. propose huge challenge.
It during existing suspension cell culture, generally requires to unscrew culture bottle cap, in addition, suspending carefully existing
During born of the same parents' dosing, often it is also required to unscrew culture bottle cap, and repeatedly unscrewing culture bottle cap this basic process will increase
Add the probability of pollution suspension cell and suspension cell culture liquid, some unpredictable difficulties are brought to subsequent experimental study
With some not confirmable factors.
The content of the invention
For in the prior art the defects of, the purpose of this utility model is to provide one kind to contribute to suspension cell carry out
Cellar culture keeps cell suspended state, prevents other microorganisms or other culture mediums from polluting the portable of suspension cell pollution
Formula suspension cell culture and chemicals dosing plant.
To achieve the above object, the utility model uses following technical scheme：
A kind of portable suspension cell culture and chemicals dosing plant, which is characterized in that including sharp bottom blake bottle, a Y
Type connecting tube and two dosers；The sharp bottom blake bottle is equipped with sharp bottom and cultivates bottle cap, and the point bottom is cultivated on bottle cap
If there are one elastic apertures；Point bottom blake bottle is installed on constant level's shaking table；The two entrances of the Y types connecting tube point
Two dosers are not connected by latex connecting tube；The latex connecting tube, which is equipped with, can be turned on and off latex connecting tube
Spring clip；The outlet of the Y types connecting tube is connected with a dosing conduit；The dosing conduit connects a dosing catheter needle
Head；The dosing catheter needle is connected through the elastic aperture with the sharp bottom blake bottle.
The portable suspension cell culture and chemicals dosing plant, wherein：The doser and the latex connecting tube
Junction, junction, the Y types connecting tube and the company of the dosing conduit of the latex connecting tube and the Y types connecting tube
Place is met, is equipped with sealing buckle.
The portable suspension cell culture and chemicals dosing plant, wherein：The volume of point bottom blake bottle is 100mL,
Its outer surface is equipped with sharp bottom blake bottle scale marker.
The portable suspension cell culture and chemicals dosing plant, wherein：The afterbody of the dosing catheter needle is equipped with one
A dosing catheter needle handle.
The portable suspension cell culture and chemicals dosing plant, wherein：The bottle cap of point bottom blake bottle is equipped with hydrophobic
Ventilated membrane.
The portable suspension cell culture and chemicals dosing plant, wherein：The inner surface of point bottom blake bottle is equipped with poly-
Tetrafluoroethene hydrophobic membrane, thus help avoid suspension cell and near-wall air curtain occur and cause death.
The portable suspension cell culture and chemicals dosing plant, wherein：The elasticity aperture is made of latex material,
It can thus ensure the sealing between medicine feeding needle and sharp bottom blake bottle.
The portable suspension cell culture and chemicals dosing plant, wherein：Piston, the piston are equipped in the doser
Even there are one the push rod with handle, the outer surface of the doser is equipped with scale marker.
Compared with prior art, the beneficial benefit of the utility model is：The utility model helps to carry out suspension cell
It cellar culture and remains that suspension cell is in suspended state, helps integrally to carry out this suspension cell point bottom blake bottle soon
Speed centrifugation is quickly collected suspension cell and is smoothed out beneficial to follow-up Basic medicine experiment, helps to prevent other microorganisms
Or other culture mediums pollute suspension cell and the Essential Environment for keeping suspension cell sterile, contribute to suspension cell in culture
Appropriate gas is kept to supply and can occur the phenomena of mortality because of anoxic to avoid suspension cell in the process, is contributed to suspending
Cell carries out one or two kinds of different type drug dosing and can be to avoid different type drug interference, finally
It will may consequently contribute to the conventional appropriate culture of suspension cell and the smooth dosing of one or two kinds of different type drug can be carried out.
Description of the drawings
Fig. 1 is the utility model structure diagram.
Fig. 2 is the structure diagram of sharp bottom culture bottle cap.
Reference sign：0- constant level's shaking table grooves；1- points bottom blake bottle；2- points bottom blake bottle scale marker；3-
Polytetrafluoroethylene (PTFE) hydrophobic membrane；Cultivate bottle cap in 4- points bottom；5- hydrophobic permeable membranes；6- dosing catheter needles；7- dosing catheter needles
Handle；8- dosing conduits；The sealing buckles of 9- the 5th；10-Y type connecting tubes；The sealing buckles of 11- first；12- the first latex connecting tubes；
The first spring clips of 13-；The sealing buckles of 14- second；The first dosers of 15- export；The first dosers of 16-；The first dosers of 17- are carved
Scale is known；18- the first doser pistons；19- the first doser pushing rams；The first dosers of 20- promote handle；21- at the uniform velocity water
Yawing bed；The sealing buckles of 22- the 3rd；23- the second latex connecting tubes；24- second springs are pressed from both sides；The sealing buckles of 25- the 4th；26-
Two dosers export；The second dosers of 27-；28- the second doser scale markers；29- the second doser pistons；30- second adds
Medicine device pushing ram；The second dosers of 31- promote handle；32- elasticity apertures；A-Y type latexes connecting tube exports；B-Y types latex connects
Take over first entrance；C-Y type latex connecting tube second entrances.
Specific embodiment
As shown in Figure 1, portable suspension cell culture and chemicals dosing plant include sharp bottom 1, Y type latex of blake bottle
Connecting tube 10, the first doser 16 and the second doser 27；Sharp bottom blake bottle 1 is installed in the at the uniform velocity water on constant level's shaking table 21
In yawing bed groove 0.Sharp bottom blake bottle 1 is equipped with sharp bottom and cultivates bottle cap 4；The madial wall of sharp bottom blake bottle 1 is equipped with polytetrafluoro
Ethylene hydrophobic membrane 3, outer surface are equipped with sharp bottom blake bottle scale marker 2；The Y types latex connecting tube 10 is equipped with two entrances and one
A outlet, (Y type latex connecting tube first entrance b and Y type latex connecting tube second entrances c) passes through the first breast to two entrances respectively
12 and second latex connecting tube 23 of glue connection pipe is connected with the first doser 16 and the second doser 27；First latex connecting tube 12
With the junction of Y type latexes connecting tube 10, the junction of the first doser 16 and the first latex connecting tube 12 is respectively equipped with first
11 and second sealing buckle 14 of sealing buckle；Second latex connecting tube 23 and the junction of Y type latexes connecting tube 10, the second dosing
The junction of 27 and second latex connecting tube 23 of device is respectively equipped with the 3rd sealing buckle 22 and the 4th sealing buckle 25；First breast
Glue connection pipe 12 is equipped with the first spring clip 13, and the second latex connecting tube 23 is equipped with second spring and presss from both sides 24；Y type latex connecting tubes
10 outlet (Y type latexes connecting tube exports a) is connected by a dosing conduit 8 with dosing catheter needle 6, dosing catheter needle
6 cultivate bottle cap 4 through sharp bottom is deep into sharp bottom blake bottle 1, the afterbody of the dosing catheter needle 6 be equipped with convenient for users to
The dosing conduit pillow handle 7 of grip.Dosing conduit 8 and the touching position of Y type latexes connecting tube 10 are equipped with the 5th sealing buckle 9.
The front end of first doser 16 exports 15 (being connected with Y type latexes connecting tube 10), the first dosing for the first doser
The outer surface of device 16 is equipped with the first doser scale marker 17, and the agent structure of the first doser 16 is internally provided with the first doser
Piston 18, the rear of the first doser piston 18 is the first doser pushing ram 19, and the rear of the first doser pushing ram 19 connects
There is the first doser to promote handle 20；The front end of second doser 27 is the outlet 26 of the second doser (with Y type latexes connecting tube 10
It is connected), the outer surface of the second doser 27 is equipped with the second doser scale marker 28, inside the agent structure of the second doser 27
Equipped with the second doser piston 29, the rear of the second doser piston 29 is the second doser pushing ram 30, and the second doser pushes away
The second doser, which is connected with, into the rear of bar 30 promotes handle 31.
As shown in Fig. 2, sharp bottom culture bottle cap 4 is equipped with hydrophobic permeable membrane 5, sharp 4 middle part of bottom culture bottle cap is equipped with latex
Elastic aperture 32 made of material；The elastic aperture 32 that dosing catheter needle 6 passes through sharp bottom to cultivate on bottle cap 4 is deep into sharp bottom
In blake bottle 1.
Latex circular hole 32 is elastic material and can be closed automatically, and very convenient suspension cell is inoculated with, is being inoculated with
When can suspension cell directly be injected into portable suspension cell culture and chemicals dosing plant using syringe, extracted out after inoculation
Syringe needle, this process need not be unscrewed in the sharp bottom culture bottle cap 4 of suspension cell culture and chemicals dosing plant, can be to avoid
There is the pollution of other microorganisms in suspension cell culture and chemicals dosing plant.Devise constant level's shaking table 21, polytetrafluoroethylene (PTFE) is dredged
Moisture film 3, hydrophobic permeable membrane 5 help to carry out suspension cell the cellar culture of aeration type and ensure that suspension cell is not adherent, with
This is avoided suspension cell from occurring death because of anoxic.Devise 10, two dosing conduit 8, Y shape connecting tube latex connecting tubes
(the first latex connecting tube 12, the second latex connecting tube 23), two spring clips (the first spring clip 13, second spring folder 24), two
A doser (the first doser 16, the second doser 27), a kind of drug can be applied to suspension cell or apply two kinds that
Non-interfering drug between this, and ensure the pollution without other microorganisms during dosing.
Such as doser (the first doser 16, the second doser 27) is respectively provided with drug A, B, when needing to carry out drug A
, it is necessary to open the first spring clip 13 and simultaneously clamp on second spring folder 24, drug A passes through the first doser at this time when dosing
16th, the first doser outlet 15, latex connecting tube 12, Y shape connecting tube first entrance b, Y shape connecting tube outlet a, dosing conduit 8,
Dosing catheter needle 6 enters suspension cell culture liquid, and this completes the dosings of drug A.
Similarly, it is necessary to open second spring folder 24 and simultaneously clamp on the first spring when needing to carry out drug B dosings
Folder 13, at this time drug B export the 26, second latex connecting tube 23, Y shape connecting tube the by the second doser 27, the second doser
Two entrance c, Y shape connecting tube outlet a, dosing conduit 8, dosing catheter needle 6 enter suspension cell culture liquid, this completes
The dosing of drug B.
Claims (8)
1. a kind of portable suspension cell culture and chemicals dosing plant, which is characterized in that including：
One sharp bottom blake bottle, a Y types connecting tube and two dosers；Point bottom blake bottle is equipped with sharp bottom blake bottle bottle
Lid, sets that there are one elastic apertures on the point bottom culture bottle cap；Point bottom blake bottle is installed on constant level's shaking table；Institute
The two entrances for stating Y type connecting tubes connect two dosers by latex connecting tube respectively；The latex connecting tube is equipped with energy
Enough it is turned on and off the spring clip of latex connecting tube；The outlet of the Y types connecting tube is connected with a dosing conduit；The dosing
Conduit connects a dosing catheter needle；The dosing catheter needle is connected through the elastic aperture with the sharp bottom blake bottle
It is logical.
2. portable suspension cell culture according to claim 1 and chemicals dosing plant, it is characterised in that：
The junction of the doser and the latex connecting tube, the latex connecting tube and the Y types connecting tube junction,
The Y types connecting tube and the junction of the dosing conduit are equipped with sealing buckle.
3. portable suspension cell culture according to claim 1 and chemicals dosing plant, it is characterised in that：
The volume of point bottom blake bottle is 100mL, and outer surface is equipped with sharp bottom blake bottle scale marker.
4. portable suspension cell culture according to claim 1 and chemicals dosing plant, it is characterised in that：
The afterbody of the dosing catheter needle is set there are one dosing catheter needle handle.
5. portable suspension cell culture according to claim 1 and chemicals dosing plant, it is characterised in that：
The bottle cap of point bottom blake bottle is equipped with hydrophobic permeable membrane.
6. portable suspension cell culture according to claim 1 and chemicals dosing plant, it is characterised in that：
The inner surface of point bottom blake bottle is equipped with polytetrafluoroethylene (PTFE) hydrophobic membrane, thus it is adherent to help avoid suspension cell appearance
Phenomenon and cause death.
7. portable suspension cell culture according to claim 1 and chemicals dosing plant, it is characterised in that：
The elasticity aperture is made of latex material, thus can ensure the sealing between medicine feeding needle and sharp bottom blake bottle.
8. portable suspension cell culture according to claim 1 and chemicals dosing plant, it is characterised in that：
Piston is equipped in the doser, even there are one the push rod with handle, the outer surface of the doser is equipped with the piston
Scale marker.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721476349.8U CN207418765U (en) | 2017-11-08 | 2017-11-08 | Portable suspension cell culture and chemicals dosing plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721476349.8U CN207418765U (en) | 2017-11-08 | 2017-11-08 | Portable suspension cell culture and chemicals dosing plant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207418765U true CN207418765U (en) | 2018-05-29 |
Family
ID=62307943
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721476349.8U Expired - Fee Related CN207418765U (en) | 2017-11-08 | 2017-11-08 | Portable suspension cell culture and chemicals dosing plant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207418765U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112522099A (en) * | 2019-09-17 | 2021-03-19 | 上海联顺生物科技有限公司 | Cell culture bottle |
-
2017
- 2017-11-08 CN CN201721476349.8U patent/CN207418765U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112522099A (en) * | 2019-09-17 | 2021-03-19 | 上海联顺生物科技有限公司 | Cell culture bottle |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180529 Termination date: 20181108 |