CN108587885B - A kind of micro-biological samples streak inoculation device and method - Google Patents
A kind of micro-biological samples streak inoculation device and method Download PDFInfo
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- CN108587885B CN108587885B CN201810369289.2A CN201810369289A CN108587885B CN 108587885 B CN108587885 B CN 108587885B CN 201810369289 A CN201810369289 A CN 201810369289A CN 108587885 B CN108587885 B CN 108587885B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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Abstract
The present invention relates to micro-biological samples processing technology fields, specifically a kind of micro-biological samples streak inoculation device and method, including culture dish, ware lid and bacterium solution injector, the bacterium solution injector includes injection pipe, injection set, spring and bacterium solution holding bottle, the injection puts on the long guiding hole being equipped with along its width direction, sealing shroud is equipped at the long guiding hole, the injection wore sealing shroud and long guiding hole and extended to inside culture dish and ware lid, and the injection set can be slided along the length direction of long guiding hole；The inner wall top surface of the ware lid is equipped with scribing line guide groove, and the injection pipe is equipped with and the scribing line matched sliding block of guide groove.Compared with the existing technology, micro-biological samples streak inoculation device and method of the present invention has the beneficial effect that the operating procedure of streak inoculation is simple, and streak inoculation is high-efficient, and fault rate is low, effectively miscellaneous bacteria can be avoided to enter, and the effect of inoculation separation bacterium colony is good.
Description
Technical field
The present invention relates to micro-biological samples processing technology field, specifically a kind of micro-biological samples streak inoculation device and side
Method.
Background technique
Plate streaking inoculation refers to the different cells in microorganism mixed in together or same micropopulation with connecing
Kind ring obtains the more individual cells being independently distributed by sectional streak dilution on plating medium surface, grows after cultivation
It is multiplied into single colonie, usually this single colonie as the purebred of microorganism to be separated.The method of existing plate streaking inoculation
Include: a, label, in culture dish bottom surface, indicates the bacterium name being inoculated with, inoculator's name, date etc. with marking pen；B, disinfection inoculation
Ring lights alcolhol burner, and the right hand holds oese, and places incineration in flame；C, strain is taken, left hand holds the examination equipped with bacterium solution
Pipe is chosen test tube plug with the right hand palm and little finger of toe for holding oese, test tube nozzle is run through flame 2~3 times and is gone out
Bacterium protrudes into oese after cooling in test tube, takes the bacterium solution of an oese, is then log out test tube, again by strain tube nozzle
It is sterilized by flame 2~3 times, is stoppered test tube plug, puts back to original position；D, streak inoculation controls ware lid with left hand thumb and index finger, left
Several fingers of remaining of hand control ware bottom, open ware lid, make angular aperture less than 30 °, in case the bacterium in air falls into wherein, and close
Flame, the right hand hold oese and back and forth cross on culture medium, paint a bacterium film.Existing plate streaking inoculation method exists
Following disadvantage: (1) it in order to as far as possible prevent miscellaneous bacteria from entering culture dish, needs to sterilize to oese and strain test tube calcination, draw
Line needs to control ware lid with left hand thumb and index finger when being inoculated with, and several fingers of remaining of left hand control ware bottom, open ware lid, make angular aperture
Less than 30 °, and close to flame, sufficiently complex, inefficiency is operated, is easy to accidentally drop and culture dish is caused to damage, miscellaneous bacteria is easy beating
Enter culture dish when opening ware lid；(2) it needs repeatedly to take strain, it is not only cumbersome, and miscellaneous bacteria is easy to cause to enter culture dish；
(3) bacterium solution volume taken out every time differs, and cannot make effectively to desalinate separation of bacterial after scribing line, influences the effect of inoculation separation bacterium colony
Fruit.
Application No. is 201420476186.3 utility model patents to disclose a kind of micro-biological samples streak inoculation device,
When in use, culture plate state in a vertical shape, the bacterium solution in liquefier cup flow into culture dish bottom through liquefier cup cannula bevel channel
The sample diversion trench of seat, is soaked in streak inoculation end in the sample of sample diversion trench, and streak inoculation pen is mechanical in scribing line
The streak inoculation on culture medium under the drive of hand.The micro-biological samples streak inoculation device does not need to open culture plate in inoculation
Lid can effectively place miscellaneous bacteria entrance, also not need using high-temperature sterilization, easy to operate, high-efficient, still, the micro-biological samples
Streak inoculation device has the disadvantage in that (1) culture plate is placed vertically, and bacterium solution can be converged under gravity in drop after scribing line
Shape can not effectively desalinate separation of bacterial, influence the effect of inoculation separation bacterium colony；(2) bacterium solution on streak inoculation pen is with scribing line
Inoculation can be fewer and fewer, and the bacterium solution film for causing scribing line to be formed is uneven.
Summary of the invention
The purpose of the present invention is to solve above-mentioned the deficiencies in the prior art, provide a kind of micro-biological samples streak inoculation dress
It sets, streak inoculation process does not need to open ware lid, and scratching process carries out inside the culture dish of relative closure, to simplify operation
Step improves streak inoculation efficiency, reduces fault rate, effectively miscellaneous bacteria is avoided to enter, in addition, bacterium solution can uniformly enter culture
Ware simultaneously forms uniform bacterium solution film, to effectively desalinate separation of bacterial, improves the effect of inoculation separation bacterium colony.
The technical problems to be solved by the invention are realized using following technical scheme: a kind of micro-biological samples streak inoculation
Device, including culture dish, ware lid and bacterium solution injector, the ware lid are fastened on culture dish；The bacterium solution injector includes note
Enter pipe, injection set, spring and bacterium solution holding bottle；The injection pipe is slidably set in injection set inside, the injection pipe position
One end inside injection set is equipped with piston, and the spring is located between injection set and piston；The end of the injection set is equipped with
Holding bottle connector, the holding bottle connector and bacterium solution holding bottle are respectively equipped with matched internal screw thread and external screw thread, and the bacterium solution is deposited
The bottle mouth position of storage bottle is equipped with sealer, is equipped with puncture portion in the holding bottle connector；The injection, which is put on, to be equipped with along its width direction
Long guiding hole, sealing shroud is equipped at the long guiding hole, one end of the sealing shroud and ware lid are tightly connected, the sealing shroud
Including rigid portion and flexible part, the rigid portion is located at the both ends of long guiding hole, and the flexible part is located at guiding length
Above and below hole, the both ends of the flexible part are connect with two rigid portions respectively, and matching is respectively equipped on two panels flexible part
Recessed bone sealing strip and convex bone sealing strip；The injection wore sealing shroud and long guiding hole and extended in culture dish and ware lid
Portion, injection set can slides along the length direction of long guiding hole, the two sides of the injection set be respectively provided with one group be used to clamp it is recessed
The clamp arm of bone sealing strip and convex bone sealing strip；The inner wall top surface of the ware lid is equipped with scribing line guide groove, sets on the injection pipe
Have and the scribing line matched sliding block of guide groove.
Technical solution of the present invention is also: the scribing line guide groove includes rake and steering portion, two neighboring rake
End pass through steering portion connect.
Technical solution of the present invention is also: the injection pipe is located at one end inside culture dish and ware lid equipped with bending part,
The outlet of the bending part is towards the inner wall bottom surface of culture dish.
Technical solution of the present invention is also: the end of the bending part is equipped with horn mouth, can be improved the effect for shakeouing bacterium solution
Fruit.
The present invention also provides a kind of micro-biological samples streak inoculation methods, comprising the following steps:
A, culture medium is inserted in culture dish, to micro-biological samples streak inoculation device high temperature sterilization, is infused on culture dish
The bacterium name of bright inoculation, inoculator's name, date；
B, bacterium solution holding bottle is connect with holding bottle joint screw thread, as bacterium solution holding bottle and the opposite of holding bottle connector move
Dynamic, sealer is pierced through in puncture portion, and bacterium solution flows into injection set；
C, bacterium solution injector is made to reciprocatingly slide along long guiding hole, the recessed bone sealing strip and convex bone sealing strip of injection set two sides exist
Engaging sealing, injection pipe opposite injection set axis under the action of crossing guide groove, sliding block and spring are kept under the action of clamp arm
To retraction, the track for exporting guide groove to the layout line of injection pipe is mobile, and bacterium solution is under the extruding of piston uniformly from injection pipe
Bacterium solution is uniformly applied to formation bacterium solution film on culture medium by outflow, injection pipe.
Compared with the existing technology, micro-biological samples streak inoculation device of the present invention has the beneficial effect that (1) streak inoculation
Journey do not need open ware lid, injection set along long guiding hole sliding during, injection set two sides recessed bone sealing strip and convex bone it is close
Strip of paper used for sealing keeps engaging to seal under the action of clamp arm, carries out streak inoculation process inside the culture dish of relative closure, only
Need to make bacterium solution injector along long guiding hole reciprocatingly slide can streak inoculation, operating procedure is simple, and streak inoculation is high-efficient, fault
Rate is low, effectively miscellaneous bacteria can be avoided to enter；(2) during streak inoculation, bacterium solution is uniformly squeezed into culture dish and shape
It at uniform bacterium solution film, does not need repeatedly to take kind, inoculation efficiency is high, and the effect of inoculation separation bacterium colony is good.
Detailed description of the invention
Fig. 1 is the perspective view of micro-biological samples streak inoculation device of the present invention.
Fig. 2 is the partial enlarged view in the portion A in Fig. 1.
Fig. 3 is the cross-sectional view of micro-biological samples streak inoculation device of the present invention.
Fig. 4 is the partial enlarged view in the portion B in Fig. 3.
Fig. 5 be Fig. 3 in C to cross-sectional view.
Fig. 6 is the structural schematic diagram of ware lid of the present invention.
Fig. 7 is the structural schematic diagram of bacterium solution injector of the present invention.
In figure: 1, culture dish, 2, ware lid, 3, injection pipe, 4, injection set, 5, spring, 6, bacterium solution holding bottle, 7, piston, 8,
Holding bottle connector, 9, puncture portion, 10, long guiding hole, 11, rigid portion, 12, flexible part, 13, recessed bone sealing strip, 14, the sealing of convex bone
Item, 15, clamp arm, 16, sliding block, 17, rake, 18, steering portion, 19, bending part, 20, horn mouth.
Specific embodiment
In order to clarify the technical characteristics of the invention, making below according to attached drawing to the specific embodiment of the invention further
Explanation.
As shown in FIG. 1 to FIG. 7, a kind of micro-biological samples streak inoculation device, including culture dish 1, ware lid 2 and bacterium solution injection
Device.Ware lid 2 is fastened on culture dish 1, and culture dish 1 and ware lid 2 are all made of transparent material and are made, and organic glass is used in the present embodiment
Glass.
As shown in Figure 3 and Figure 7, bacterium solution injector includes injection pipe 3, injection set 4, spring 5 and bacterium solution holding bottle 6.Injection
Pipe 3 is slidably set in inside injection set 4, and one end that injection pipe 3 is located inside injection set 4 is equipped with piston 7, and spring 5 is located at
Between injection set 4 and piston 7.
The end of injection set 4 is equipped with holding bottle connector 8, and holding bottle connector 8 and bacterium solution holding bottle 6 are respectively equipped in matched
Screw thread and external screw thread, bacterium solution holding bottle 6 is transparent material and is equipped with scale, to observe bacterium solution injection rate.Bacterium solution holding bottle 6
Bottle mouth position is equipped with sealer, and the puncture portion 9 for puncturing sealer is equipped in holding bottle connector 8.
Injection set 4 is equipped with the long guiding hole 10 along its width direction, is equipped with sealing shroud at long guiding hole 10, sealing shroud
One end and ware lid 2 are tightly connected.As shown in Figure 1, Figure 2, shown in Fig. 4 and Fig. 5, sealing shroud includes rigid portion 11 and flexible part 12, rigidity
Portion 11 is located at the both ends of long guiding hole 10, and flexible part 12 is located above and below long guiding hole 10, flexible part 12
Both ends connect respectively with two rigid portions 11, matched recessed bone sealing strip 13 is respectively equipped on two panels flexible part 12 and convex bone is close
Strip of paper used for sealing 14.Injection set 4 passes through sealing shroud and long guiding hole 10 and extends to inside culture dish 1 and ware lid 2, and injection set 4 can be along leading
It is slided to the length direction of long hole 10, the two sides of injection set 4 are respectively provided with one group for clamping recessed bone sealing strip 13 and the sealing of convex bone
The clamp arm 15 of item 14.
As shown in fig. 6, the inner wall top surface of ware lid 2 is equipped with scribing line guide groove, scribing line guide groove includes rake 17 and turns to
The end in portion 18, two neighboring rake 17 is connected by steering portion 18.Injection pipe 3 is equipped with and the scribing line matched cunning of guide groove
Block 16.
As shown in figure 3, injection pipe 3, which is located at one end inside culture dish 1 and ware lid 2, is equipped with bending part 19, bending part 19
End is equipped with horn mouth 20, and horn mouth 20 is towards the inner wall bottom surface of culture dish 1.
The present invention also provides a kind of methods of micro-biological samples streak inoculation, comprising the following steps:
A, culture medium is inserted in culture dish 1, to micro-biological samples streak inoculation device high temperature sterilization of the invention, trained
Support the bacterium name that inoculation is indicated on ware 1, inoculator's name, date；
B, bacterium solution holding bottle 6 is threadedly coupled with holding bottle connector 8, with the phase of bacterium solution holding bottle 6 and holding bottle connector 8
To movement, sealer is pierced through in puncture portion 9, and bacterium solution flows into injection set 4；
C, bacterium solution injector is made to reciprocatingly slide along long guiding hole 10, the recessed bone sealing strip 13 and convex bone of 4 two sides of injection set are close
Strip of paper used for sealing 14 keeps engaging to seal under the action of clamp arm 15, effect of the injection pipe 3 in scribing line guide groove, sliding block 16 and spring 5
The track of 4 axial retractions of lower opposite injection set, the guide groove to the layout line of horn mouth 20 is mobile, bacterium solution under the extruding of piston 7
Even to flow out from horn mouth 20, bacterium solution is uniformly applied to formation bacterium solution film on culture medium by horn mouth 20.
The embodiment of the present invention is elaborated above in conjunction with attached drawing, but the present invention is not limited to above-mentioned implementations
Example, within the knowledge of a person skilled in the art, can also do without departing from the purpose of the present invention
Various change out.
Claims (5)
1. a kind of micro-biological samples streak inoculation device, including culture dish (1) and ware lid (2), the ware lid (2) are fastened on culture
On ware (1), it is characterised in that: further include bacterium solution injector, the bacterium solution injector includes injection pipe (3), injection set (4), bullet
Spring (5) and bacterium solution holding bottle (6)；The injection pipe (3) is slidably set in injection set (4) inside, injection pipe (3) position
Piston (7) are equipped in the internal one end of injection set (4), the spring (5) is located between injection set (4) and piston (7)；The note
The end for entering set (4) is equipped with holding bottle connector (8), and the holding bottle connector (8) is respectively equipped with matched with bacterium solution holding bottle (6)
Internal screw thread and external screw thread, the bottle mouth position of the bacterium solution holding bottle (6) are equipped with sealer, are equipped with and puncture in the holding bottle connector (8)
Portion (9)；The injection set (4) is equipped with the long guiding hole (10) along its width direction, is equipped at the long guiding hole (10) close
Big envelope, one end of the sealing shroud and ware lid (2) are tightly connected, and the sealing shroud includes rigid portion (11) and flexible part (12),
The rigid portion (11) is located at the both ends of long guiding hole (10), and the flexible part (12) is located at long guiding hole (10)
Above and below, the both ends of the flexible part (12) are connect with two rigid portions (11) respectively, on two panels flexible part (12) respectively
Equipped with matched recessed bone sealing strip (13) and convex bone sealing strip (14)；The injection set (4) passes through sealing shroud and long guiding hole
(10) and culture dish (1) and ware lid (2) inside are extended to, the injection set (4) can be sliding along the length direction of long guiding hole (10)
Dynamic, the two sides of the injection set (4) are respectively provided with one group for clamping the clamping of recessed bone sealing strip (13) and convex bone sealing strip (14)
Arm (15)；The inner wall top surface of the ware lid (2) is equipped with scribing line guide groove, and the injection pipe (3) is equipped with and scribing line guide groove
The sliding block (16) matched.
2. micro-biological samples streak inoculation device according to claim 1, it is characterised in that: the scribing line guide groove includes
The end of rake (17) and steering portion (18), two neighboring rake (17) is connected by steering portion (18).
3. micro-biological samples streak inoculation device according to claim 1, it is characterised in that: the injection pipe (3) is located at
The internal one end of culture dish (1) and ware lid (2) is equipped with bending part (19), and the outlet of the bending part (19) is towards culture dish (1)
Inner wall bottom surface.
4. micro-biological samples streak inoculation device according to claim 3, it is characterised in that: the end of the bending part (19)
Portion is equipped with horn mouth (20).
It is drawn 5. a kind of micro-biological samples streak inoculation device using as described in Claims 1 to 4 is any carries out micro-biological samples
The method of line inoculation, comprising the following steps:
A, culture medium is inserted in culture dish (1), to micro-biological samples streak inoculation device high temperature sterilization, on culture dish (1)
Indicate bacterium name, the inoculator's name, date of inoculation；
B, bacterium solution holding bottle (6) is threadedly coupled with holding bottle connector (8), with bacterium solution holding bottle (6) and holding bottle connector (8)
Relative movement, puncture portion (9) pierce through sealer, bacterium solution flow into injection set (4) in；
C, bacterium solution injector is made to reciprocatingly slide along long guiding hole (10), the recessed bone sealing strip (13) of injection set (4) two sides and convex bone
Sealing strip (14) keeps engaging to seal under the action of clamp arm (15), and injection pipe (3) is in scribing line guide groove, sliding block (16) and bullet
Opposite injection set (4) axial retraction under the action of spring (5), the track for exporting guide groove to the layout line of injection pipe (3) is mobile, bacterium
Uniformly bacterium solution is uniformly applied on culture medium liquid by outflow, injection pipe (3) from injection pipe (3) under the extruding of piston (7)
Form bacterium solution film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810369289.2A CN108587885B (en) | 2018-04-23 | 2018-04-23 | A kind of micro-biological samples streak inoculation device and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810369289.2A CN108587885B (en) | 2018-04-23 | 2018-04-23 | A kind of micro-biological samples streak inoculation device and method |
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| Publication Number | Publication Date |
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| CN108587885A CN108587885A (en) | 2018-09-28 |
| CN108587885B true CN108587885B (en) | 2019-03-01 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110885745A (en) * | 2019-11-27 | 2020-03-17 | 邳州高新区生物医药研究院有限公司 | Inoculation culture dish for biological medicine |
| CN110982679B (en) * | 2020-01-08 | 2020-11-27 | 山东瑞谱检测技术有限公司 | Inoculation device for microbiological assay |
| CN113980791B (en) * | 2021-11-30 | 2024-01-12 | 深圳零一生命科技有限责任公司 | Automatic microorganism inoculation instrument and inoculation scribing method thereof |
| CN114456919B (en) * | 2022-03-17 | 2022-09-16 | 南方海洋科学与工程广东省实验室(广州) | High-pressure environment marine microorganism solid separation culture device and culture method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103194385B (en) * | 2013-03-14 | 2014-04-23 | 华珊微生物科技有限公司 | Microbial sample processing system |
| CN204022831U (en) * | 2014-08-22 | 2014-12-17 | 华珊微生物科技有限公司 | A kind of micro-biological samples streak inoculation device |
| CN104403938B (en) * | 2014-12-04 | 2016-08-24 | 威海百博医疗智能机器人有限公司 | Micro-biological samples processes intelligent robot |
| CN104974932B (en) * | 2015-07-16 | 2018-04-03 | 重庆庞通医疗器械有限公司 | microorganism streak inoculation instrument |
| CN105886392A (en) * | 2016-06-14 | 2016-08-24 | 重庆庞通医疗器械有限公司 | Gear drive type microorganism streak inoculation instrument |
| CN105886383A (en) * | 2016-06-14 | 2016-08-24 | 重庆庞通医疗器械有限公司 | Microorganism streak inoculation instrument |
| CN207157935U (en) * | 2017-09-05 | 2018-03-30 | 源野茶香（广州）饮料食品有限公司 | Press puncturing structure and beverage bottle |
| CN107475097A (en) * | 2017-09-08 | 2017-12-15 | 安徽圣田生物科技有限公司 | A kind of microbiological culture media is inoculated with structure |
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