CN216947047U

CN216947047U - Immune cell culture device

Info

Publication number: CN216947047U
Application number: CN202122820443.3U
Authority: CN
Inventors: 廖联明; 刘璐; 廉云飞; 毛慧玲
Original assignee: Jiangsu Tuohong Kangheng Pharmaceutical Co ltd
Current assignee: Jiangsu Tuohong Kangheng Pharmaceutical Co ltd
Priority date: 2021-11-17
Filing date: 2021-11-17
Publication date: 2022-07-12
Anticipated expiration: 2031-11-17

Abstract

The utility model provides a culture device for immune cells, which belongs to the technical field of immune cell culture and comprises an incubator, wherein the left side of the incubator is movably connected with a chamber door, the middle part of the chamber door is provided with an observation window, the inner bottom wall of the incubator is fixedly connected with a support column, and the top of the support column is fixedly connected with a support table. The utility model solves the problems that a plurality of culture dishes are arranged in a culture device, the culture device needs to be taken for a plurality of times when the acidity and alkalinity of the culture medium are detected, the operation is troublesome, and the pollution probability of the culture device is increased to influence the culture of immune cells.

Description

Immune cell culture device

The technical field is as follows:

the utility model belongs to the technical field of immune cell culture, and particularly relates to a culture device for immune cells.

Background art:

the cell culture refers to a method for simulating in-vivo environment (sterility, proper temperature, pH value, certain nutritional conditions and the like) in vitro to enable the in-vivo environment to survive, grow, reproduce and maintain main structure and functions.

In the process of immune cell culture, a culture medium liquid needs to be added, substances required by cell growth are mainly supplied by the culture medium, but the cells grow vigorously and are metabolized actively, a large amount of lactic acid and CO2 are generated, the acidity and alkalinity of the culture medium are influenced, the acidity and alkalinity of the culture medium influence the cell culture, and the acidity and alkalinity of the culture medium need to be detected.

The utility model has the following contents:

the utility model provides a culture device for immune cells, which aims to solve the problems that a plurality of culture dishes are arranged in the culture device, the culture device needs to be taken for a plurality of times when the pH value of a culture medium is detected, the operation is troublesome, the pollution probability of the culture device is increased, and the culture of the immune cells is influenced.

The utility model provides an immune cell culture device which comprises an incubator, wherein a chamber door is movably connected to the left side of the incubator, an observation window is installed in the middle of the chamber door, a support column is fixedly connected to the inner bottom wall of the incubator, a support table is fixedly connected to the top of the support column, culture dishes which are uniformly distributed are placed on the top of the support table, a seal cover is movably connected to the top of the incubator, an outer casing is fixedly connected to the middle of the top of the seal cover, a sliding casing is slidably connected to the inner side of the outer casing, an absorbing mechanism is arranged on the inner side of the sliding casing, a sliding ring is fixedly connected to the outer side of the sliding casing, springs which are symmetrically distributed are arranged at the bottom of the sliding ring, and a sampling mechanism is arranged at the bottom of the outer casing.

Further, the suction mechanism comprises a suction shell, a pull rod, a piston plate, a pull block and a connecting ring, the suction shell is located on the inner side of the sliding shell and is in sliding connection with the inner side of the sliding shell, the pull rod is in sliding connection with the top of the suction shell, the pull rod penetrates through the top of the suction shell, the top of the piston plate is fixedly connected with the bottom of the pull rod, the bottom of the pull block is fixedly connected with the top of the pull rod, and the connecting ring is fixedly connected with the bottom of the suction shell.

Through adopting above-mentioned technical scheme, utilize suction means cooperation sampling mechanism, the pulling draws the piece to drive pull rod, piston board and removes, can absorb the air in the chamber of ventilating, can absorb the culture medium in the culture dish through sampling mechanism.

Further, the piston plate is in close contact with and is in sliding connection with the inner side wall of the suction shell, an opening is formed in the bottom of the suction shell and communicated with the inner side of the connecting ring, and an annular bump is fixedly connected to the outer side of the suction shell.

Through adopting above-mentioned technical scheme, utilize the piston board, when the piston board slides, can with the gas in the chamber of ventilating through inhaling in absorbing the shell, form the negative pressure with the culture medium inhale the sample glass pipe in, make things convenient for the culture medium collective in a plurality of culture dishes to detect.

Further, sampling mechanism includes adapter sleeve, valve, sampling dish, a plurality of chamber of ventilating, a plurality of sample glass pipe, a plurality of connecting cutting ferrule, a plurality of flexure strip, a plurality of draw-in groove, concentrated chamber, the inboard bottom threaded connection of adapter sleeve and slip shell, the valve cup joints the outside at the adapter sleeve, the bottom fixed connection of sampling dish and adapter sleeve, it is a plurality of ventilate the chamber and set up in the inboard and evenly distributed of sampling dish, it is a plurality of sample glass pipe evenly distributed is in the bottom of sampling dish, and is a plurality of connecting cutting ferrule respectively with the top fixed connection of a plurality of sample glass pipe, and is a plurality of the outside of connecting cutting ferrule all is provided with four flexure strips, and is a plurality of the diapire of sampling dish is seted up to the draw-in groove and is matchd with the flexure strip, concentrate the chamber and set up in the inside of sampling dish.

Through adopting above-mentioned technical scheme, utilize sampling mechanism to take a sample the culture medium of placing in the culture dish on the brace table to can pull down sampling mechanism, need not take many times, easy operation conveniently detects the pH value of the culture medium in the culture dish.

Furthermore, the connecting clamping sleeve is communicated with the inner side of the sampling glass tube, the four elastic sheets are symmetrically distributed and fixedly connected with the outer side of the connecting clamping sleeve, a plurality of communicating holes matched with the connecting clamping sleeve are formed in the bottom of the sampling disc, and the clamping grooves are located on the inner side of the communicating holes.

Through adopting above-mentioned technical scheme, utilize the flexure strip can make things convenient for being connected of connecting cutting ferrule and sampling disc, and then make things convenient for the dismantlement and the change of sample glass pipe, utilize sample glass pipe and connecting cutting ferrule intercommunication, ventilate chamber and connecting cutting ferrule intercommunication, make suction means when the operation, can exert on sample glass pipe.

Furthermore, the outer side of the top of the connecting clamping sleeve is fixedly connected with a sealing ring, and the concentration cavity is communicated with the plurality of ventilation cavities.

Through adopting above-mentioned technical scheme, utilize the sealing washer can guarantee the leakproofness that connecting cutting ferrule and sampling disc are connected, guarantee the absorption of sample glass pipe to the culture medium.

Further, the number of the vent cavities is the same as that of the sampling glass tubes and the culture dishes.

Through adopting above-mentioned technical scheme, guarantee that quantity is the same, can carry out sample detection to the culture medium in a plurality of culture dishes simultaneously, reduce staff's work load, need not take many times.

Furthermore, a placing groove matched with the culture dish is formed in the top of the supporting table, an annular sliding groove matched with the sliding ring is formed in the inner side of the outer sleeve, the top of the spring is fixedly connected with the bottom of the sliding ring, and the bottom of the spring is fixedly connected with the inner side of the outer sleeve.

Through adopting above-mentioned technical scheme, utilize the standing groove can stabilize the culture dish, utilize slip ring, spring to make the slip shell slide and then control the distance of sampling mechanism and culture dish, when not needing the sample, the culture dish is kept away from to sampling mechanism, can not lead to the fact the influence to the immune cell culture in the culture dish.

Compared with the prior art, the technical scheme of the utility model has the following beneficial technical effects:

1. according to the utility model, through the cooperation of the sampling glass tube, the sampling disc, the suction mechanism, the sampling mechanism and the like, the pull block is pulled, the pull block drives the pull rod and the piston plate, when the piston plate moves, gas in the concentration cavity and the ventilation cavity is sucked into the suction shell, culture mediums in the culture dishes are sucked into the sampling glass tube, the culture mediums in a plurality of culture dishes are sampled at one time, the culture dishes do not need to be taken out, and different culture dishes do not need to be taken out respectively, so that the time of contact with the outside is reduced, the culture mediums are directly sampled on the inner side of the culture box, the box door of the culture box does not need to be opened for multiple times and for a long time, the operation is simple, the pollution probability of a culture device is reduced, and the culture of immune cells is ensured.

2. According to the utility model, through the cooperation of the elastic sheet, the connecting clamping sleeve, the clamping groove, the sampling glass tube and the like, the sampling glass tube can be quickly replaced through the elastic sheet and can be repeatedly used, and the next sampling detection is convenient.

Additional features and advantages of the utility model will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by the practice of the utility model. The objectives and other advantages of the utility model may be realized and attained by the structure particularly pointed out in the written description and drawings.

Description of the drawings:

the accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the principles of the utility model and not to limit the utility model. In the drawings:

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic cross-sectional top view of a sampling plate according to the present invention;

FIG. 3 is an enlarged view of the structure at A in FIG. 1;

FIG. 4 is an enlarged view of the structure at B in FIG. 1;

FIG. 5 is an enlarged view of a part of the structure of the present invention.

Reference numerals: 1. an incubator; 2. a box door; 3. an observation window; 4. a support pillar; 5. a support table; 6. a culture dish; 7. sealing the cover; 8. an outer casing; 9. a sliding housing; 10. a slip ring; 11. a spring; 12. a suction mechanism; 121. sucking a shell; 122. a pull rod; 123. a piston plate; 124. pulling the block; 125. a connecting ring; 13. a sampling mechanism; 131. connecting sleeves; 132. a valve; 133. a sampling tray; 134. a vent lumen; 135. sampling a glass tube; 136. connecting the clamping sleeve; 137. an elastic sheet; 138. a card slot; 139. a concentration chamber.

The specific implementation mode is as follows:

in order to make the objects, technical solutions and advantages of the technical solutions of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the drawings of the specific embodiments of the present invention. Like reference symbols in the various drawings indicate like elements. It should be noted that the described embodiments are only some embodiments of the utility model, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the utility model without any inventive step, are within the scope of protection of the utility model.

As shown in fig. 1-5, the present invention provides an immune cell culture apparatus, which includes an incubator 1, a chamber door 2 is movably connected to the left side of the incubator 1, an observation window 3 is installed in the middle of the chamber door 2, a support column 4 is fixedly connected to the inner bottom wall of the incubator 1, a support table 5 is fixedly connected to the top of the support column 4, culture dishes 6 are placed on the top of the support table 5, a seal cover 7 is movably connected to the top of the incubator 1, an outer casing 8 is fixedly connected to the middle of the top of the seal cover 7, a sliding casing 9 is slidably connected to the inner side of the outer casing 8, an absorption mechanism 12 is disposed on the inner side of the sliding casing 9, a sliding ring 10 is fixedly connected to the outer side of the sliding casing 9, springs 11 are symmetrically disposed on the bottom of the sliding ring 10, and a sampling mechanism 13 is disposed on the bottom of the outer casing 8.

As shown in fig. 1 and 4, the suction mechanism 12 includes a suction housing 121, a pull rod 122, a piston plate 123, a pull block 124, and a connection ring 125, the suction housing 121 is located inside the sliding housing 9 and is slidably connected to the inside of the sliding housing 9, the pull rod 122 is slidably connected to the top of the suction housing 121, the pull rod 122 penetrates through the top of the suction housing 121, the top of the piston plate 123 is fixedly connected to the bottom of the pull rod 122, the bottom of the pull block 124 is fixedly connected to the top of the pull rod 122, and the connection ring 125 is fixedly connected to the bottom of the suction housing 121, the suction mechanism 12 is used to cooperate with the sampling mechanism 13, the pull block 124 is pulled to drive the pull rod 122 and the piston plate 123 to move, so as to suck air in the ventilation cavity 134, and the culture medium in the culture dish 6 can be sucked by the sampling mechanism 13;

the piston plate 123 is in close contact and sliding connection with the inner side wall of the suction shell 121, the bottom of the suction shell 121 is provided with an opening, the opening is communicated with the inner side of the connecting ring 125, the outer side of the suction shell 121 is fixedly connected with an annular bump, and by means of the piston plate 123, when the piston plate 123 slides, gas in the ventilation cavity 134 can be sucked into the suction shell 121 through the connecting ring 125, negative pressure is formed to suck a culture medium into the sampling glass tube 135, and the collective detection of the culture medium in a plurality of culture dishes is facilitated;

as shown in fig. 1-3 and 5, the sampling mechanism 13 includes a connection sleeve 131, a valve 132, a sampling disc 133, a plurality of vent cavities 134, a plurality of sampling glass tubes 135, a plurality of connection clamp sleeves 136, a plurality of elastic sheets 137, a plurality of clamp grooves 138, and a concentration cavity 139, the connection sleeve 131 is in threaded connection with the bottom of the inner side of the sliding housing 9, the valve 132 is sleeved on the outer side of the connection sleeve 131, the sampling disc 133 is fixedly connected with the bottom of the connection sleeve 131, the plurality of vent cavities 134 are arranged on the inner side of the sampling disc 133 and are uniformly distributed, the plurality of sampling glass tubes 135 are uniformly distributed on the bottom of the sampling disc 133, the plurality of connection clamp sleeves 136 are respectively fixedly connected with the tops of the plurality of sampling glass tubes 135, the outer sides of the plurality of connection clamp sleeves 136 are provided with four elastic sheets 137, the plurality of clamp grooves 138 are arranged on the bottom wall of the sampling disc 133 and are matched with the elastic sheets 137, the concentration cavity 139 is arranged inside the sampling disc 133, the sampling mechanism 13 can be used for sampling the culture medium in the culture dish 6 placed on the support table 5, and the sampling mechanism 13 can be detached without taking the culture medium for many times, so that the operation is simple, and the detection of the pH value of the culture medium in the culture dish 6 is convenient;

the connecting clamping sleeve 136 is communicated with the inner side of the sampling glass tube 135, the four elastic sheets 137 are symmetrically distributed and fixedly connected with the outer side of the connecting clamping sleeve 136, the bottom of the sampling disc 133 is provided with a plurality of communicating holes matched with the connecting clamping sleeve 136, the clamping grooves 138 are positioned on the inner side of the communicating holes, the elastic sheets 137 can be used for facilitating the connection of the connecting clamping sleeve 136 and the sampling disc 133, further facilitating the disassembly and replacement of the sampling glass tube 135, and the sampling glass tube 135 is communicated with the connecting clamping sleeve 136 and the ventilation cavity 134 is communicated with the connecting clamping sleeve 136, so that the suction mechanism 12 can act on the sampling glass tube 135 when in operation;

the outer side of the top of the connecting clamping sleeve 136 is fixedly connected with a sealing ring, the concentration cavity 139 is communicated with the plurality of vent cavities 134, the sealing ring can ensure the connection tightness of the connecting clamping sleeve 136 and the sampling disc 133, and the suction of the sampling glass tube 135 to the culture medium;

the number of the vent cavities 134, the sampling glass tubes 135 and the culture dishes 6 is the same, so that the number is ensured to be the same, the culture mediums in the plurality of culture dishes 6 can be sampled and detected at the same time, the workload of workers is reduced, and the workers do not need to take the culture mediums for many times;

through fig. 1 and 4, a placing groove matched with the culture dish 6 is formed in the top of the supporting table 5, an annular sliding groove matched with the sliding ring 10 is formed in the inner side of the outer casing 8, the top of the spring 11 is fixedly connected with the bottom of the sliding ring 10, the bottom of the spring 11 is fixedly connected with the inner side of the outer casing 8, the culture dish 6 can be stabilized by the placing groove, the sliding ring 10 and the spring 11 can enable the sliding casing 9 to slide so as to control the distance between the sampling mechanism 13 and the culture dish 6, when sampling is not needed, the sampling mechanism 13 is far away from the culture dish 6, and immune cell culture in the culture dish 6 cannot be influenced.

The implementation mode is specifically as follows: when cell culture is carried out, the sealing cover 7 is in a closed state, the valve 132 is in an open state, when the acidity and alkalinity of the culture medium in the culture dish 6 are regularly detected, the sealing cover 7 is opened, the suction mechanism 12 is inserted into the sliding shell 9, when the annular bump is contacted with the top of the sliding shell 9, the connecting ring 125 is inserted into the top of the connecting sleeve 131 at the moment, the suction shell 121 is continuously pressed to drive the sliding shell 9 to descend, the spring 11 contracts at the moment, the sliding shell 9 descends to drive the sampling mechanism 13 to descend, when the sampling mechanism 13 descends, the bottom of the sampling glass tube 135 extends into the culture medium liquid in the culture dish 6 from the top of the culture dish 6 to be contacted, at the moment, the pull block 124 is pulled, the pull rod 122 and the piston plate 123 are driven by the pull block 124, when the piston plate 123 moves, the gas in the concentration cavity 139 and the ventilation cavity 134 is sucked into the suction shell 121, the culture medium in the culture dish 6 is sucked into the sampling glass tube 135, the culture mediums in the plurality of culture dishes 6 are sampled at one time, different culture dishes 6 are not required to be respectively taken out, the time of contact with the outside is reduced, the culture mediums are directly sampled on the inner side of the incubator 1, the chamber door 2 is opened at the moment, the valve 132 is closed, the connecting sleeve 131 is rotated to separate the sampling mechanism 13 from the sliding shell 9, the sampling mechanism 13 can be taken out, the chamber door 2 is closed at the moment, the bottom of the sampling glass tubes 135 of the sampling mechanism 13 taken out are respectively aligned to a plurality of test tubes with phenol red indicators prepared outside, the valve 132 is opened at the moment, gas enters, the sampling culture mediums in the sampling glass tubes 135 flow out from the sampling glass tubes 135 to enter the test tubes with phenol red indicators, the test tubes with phenol red indicators can be observed by workers to carry out color observation, the acid-base property of the culture mediums is judged, the culture dishes 6 of the culture mediums exceeding the standard are treated, connecting cutting ferrule 136 and sampling dish 133's being connected, inserting the intercommunicating pore of sampling dish 133 diapire through flexure strip 137, flexure strip 137 enters into draw-in groove 138, can guarantee to sample glass pipe 135 and sampling dish 133 be connected to sample glass pipe 135 can carry out quick replacement through flexure strip 137, makes things convenient for the sample detection next time.

The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the utility model as claimed. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides an immune cell culture device, includes incubator (1), its characterized in that, the left side swing joint of incubator (1) has chamber door (2), the mid-mounting of chamber door (2) has observation window (3), the interior diapire fixedly connected with support column (4) of incubator (1), the top fixedly connected with brace table (5) of support column (4), evenly distributed culture dish (6) have been placed at the top of brace table (5), the top swing joint of incubator (1) has closing cap (7), the top middle part fixedly connected with overcoat shell (8) of closing cap (7), the inboard sliding connection of overcoat shell (8) has slide shell (9), the inboard of slide shell (9) is provided with suction means (12), the outside fixedly connected with slip ring (10) of slide shell (9), the bottom of slip ring (10) is provided with symmetrical distribution's spring (11), the bottom of overcoat shell (8) is provided with sampling mechanism (13).

2. The device for culturing an immune cell according to claim 1, wherein: absorb mechanism (12) including absorbing shell (121), pull rod (122), piston plate (123), draw piece (124), go-between (125), absorb shell (121) be located the inboard of slip shell (9) and with the inboard sliding connection of slip shell (9), pull rod (122) and the top sliding connection who absorbs shell (121), pull rod (122) run through the top of absorbing shell (121), the top of piston plate (123) and the bottom fixed connection of pull rod (122), the bottom of drawing piece (124) and the top fixed connection of pull rod (122), go-between (125) and the bottom fixed connection who absorbs shell (121).

3. An apparatus for culturing an immune cell according to claim 2, wherein: the piston plate (123) is in close contact with and is in sliding connection with the inner side wall of the suction shell (121), an opening is formed in the bottom of the suction shell (121), the opening is communicated with the inner side of the connecting ring (125), and an annular convex block is fixedly connected to the outer side of the suction shell (121).

4. The device for culturing an immune cell according to claim 1, wherein: the sampling mechanism (13) comprises a connecting sleeve (131), a valve (132), a sampling disc (133), a plurality of ventilation cavities (134), a plurality of sampling glass tubes (135), a plurality of connecting clamping sleeves (136), a plurality of elastic sheets (137), a plurality of clamping grooves (138) and a concentration cavity (139), the connecting sleeve (131) is in threaded connection with the bottom of the inner side of the sliding shell (9), the valve (132) is sleeved on the outer side of the connecting sleeve (131), the sampling disc (133) is fixedly connected with the bottom of the connecting sleeve (131), the ventilation cavities (134) are arranged on the inner side of the sampling disc (133) and are uniformly distributed, the sampling glass tubes (135) are uniformly distributed at the bottom of the sampling disc (133), the connecting clamping sleeves (136) are respectively fixedly connected with the tops of the sampling glass tubes (135), and four elastic sheets (137) are arranged on the outer side of the connecting sleeve (136), a plurality of draw-in groove (138) are seted up in the diapire of sampling dish (133) and are matched with flexure strip (137), concentrated chamber (139) are seted up in the inside of sampling dish (133).

5. The device for culturing an immune cell according to claim 4, wherein: the connecting clamping sleeve (136) is communicated with the inner side of the sampling glass tube (135), the four elastic sheets (137) are symmetrically distributed and fixedly connected with the outer side of the connecting clamping sleeve (136), the bottom of the sampling disc (133) is provided with a plurality of communicating holes matched with the connecting clamping sleeve (136), and the clamping grooves (138) are located on the inner side of the communicating holes.

6. The device for culturing an immune cell according to claim 4, wherein: the outer side of the top of the connecting clamping sleeve (136) is fixedly connected with a sealing ring, and the concentration cavity (139) is communicated with the plurality of vent cavities (134).

7. The device for culturing an immune cell according to claim 4, wherein: the number of the vent cavities (134) is the same as that of the sampling glass tubes (135) and the culture dishes (6).

8. An apparatus for culturing an immune cell according to claim 1, wherein: the top of the support table (5) is provided with a placing groove matched with the culture dish (6), the inner side of the outer sleeve shell (8) is provided with an annular sliding groove matched with the sliding ring (10), the top of the spring (11) is fixedly connected with the bottom of the sliding ring (10), and the bottom of the spring (11) is fixedly connected with the inner side of the outer sleeve shell (8).