CN115251047A

CN115251047A - Based on ecological case is used in biological sample storage of cell

Info

Publication number: CN115251047A
Application number: CN202211015681.XA
Authority: CN
Inventors: 曾峰
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-08-24
Filing date: 2022-08-24
Publication date: 2022-11-01
Anticipated expiration: 2042-08-24
Also published as: CN115251047B

Abstract

The invention discloses an ecological box for storing biological samples based on cells, which relates to the technical field of ecological boxes, and comprises a bottom plate and is characterized in that: the storage box is fixedly arranged on one side of the bottom plate, a storage groove is formed in the storage box, and one side of the storage box is fixedly connected with the top plate; set up the storage mechanism in holding tank, including bearing the roller, bearing the roller and the holding tank matching activity sets up, bearing roller one side and seting up the spacing groove, set up the air guide subassembly between the protruding groove, bearing roller one end sets up sealing component, the mounting groove is seted up to storage box one end, fixed carbon dioxide storage jar and the oxygen jar of setting up respectively on the bottom plate of air heater both sides, and with the air heater between the intercommunication set up, air heater and air guide subassembly intercommunication set up, the storage box lateral wall sets up detecting element, can realize the quick switching of environmental simulation and sealed storage mode automatically, and can realize independent environmental simulation test, the sample environment in a plurality of holding tanks does not influence each other.

Description

Based on ecological case is used in biological sample storage of cell

Technical Field

The invention relates to the technical field of ecological boxes, in particular to an ecological box for storing biological samples based on cells.

Background

Cellular organisms are all organisms with a cellular structure, which is divided into two groups according to whether the cells constituting the organism are cell nuclei coated with or without nuclear membranes: prokaryotes composed of prokaryotic cells and eukaryotes composed of eukaryotes (which are not a classification method in biological taxonomy), the conventional cell biological tissue samples are generally stored by low-temperature refrigeration after being sampled, but are cultured in a nutrient solution when the growth rule and other researches are required.

Traditional storage box generally needs ultra-low temperature cold storage, and this kind of mode lets cell biology keep the sample activity under low temperature environment, then transports, tests under suitable environment, but this kind of mode power consumption is higher, transports for a long time when can not providing low temperature environment, can not maintain better ecological environment, and then can influence cell biological activity, causes the loss.

Disclosure of Invention

The invention provides an ecological box for storing biological samples based on cells, which solves the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme:

the utility model provides an ecological box is used in biological sample storage based on cell, includes the bottom plate, still includes:

the storage box is fixedly arranged on one side of the bottom plate, a storage groove is formed in the storage box, and one side of the storage box is fixedly connected with the top plate; the storage mechanism arranged in the storage tank comprises a bearing roller, the bearing roller and the storage tank are movably matched, one side of the bearing roller is provided with a limiting groove, one side of the limiting groove is fixedly connected with a rubber sleeve, a test tube is movably arranged in the limiting groove on one side of the rubber sleeve, one end of the test tube is sleeved with a sealing sleeve, two ends of the sealing sleeve are provided with communicating grooves, one side of the sealing sleeve between the communicating grooves is fixedly connected with a reset piece, one side of the reset piece is fixedly connected with a valve block, one end of the valve block is provided with an air guide groove, the air guide groove is of an I-shaped structure, the valve block is slidably connected with the sealing sleeve, one end of the valve block is rotatably connected with a roller wheel, a storage box on one side of the storage tank is internally provided with a raised groove, set up the air guide subassembly between the protruding groove, bearing roller one end sets up sealing component, the mounting groove is seted up to storage box one end, sets up the air heater in the mounting groove, air heater and bottom plate fixed connection, fixed carbon dioxide storage jar and the oxygen jar of setting respectively on the bottom plate of air heater both sides, and with the intercommunication setting between the air heater, the air heater sets up with air guide subassembly intercommunication, the storage box lateral wall sets up determine module, under the storage environment, through rotating bearing roller drive test tube and rotate, can realize the quick switching of environmental simulation and sealed storage mode automatically, and can realize independent environmental simulation test, the sample environment in a plurality of holding tanks does not influence each other.

As a preferred technical scheme of the invention, the air guide assembly comprises an air guide pipe fixedly arranged in the storage box, two sides of the air guide pipe are fixedly connected with transfer pipes, one end of each transfer pipe is fixedly connected with an electronic valve, an air outlet end of each electronic valve is communicated with the corresponding bulge groove, and the electronic valves can flexibly control the conveying and sealing of air transmitted from the transfer pipes.

As a preferable technical scheme of the invention, the sealing assembly comprises a sealing cover fixedly arranged on one side of the bearing roller, a sealing gasket is fixedly arranged on the inner side of the sealing cover, one end of the sealing cover is provided with a positioning rod in a threaded penetrating manner, the side wall of the storage box on one side of the storage tank is provided with a positioning groove, and the positioning groove is matched with the positioning rod.

According to a preferable technical scheme of the invention, the detection assembly comprises an installation frame fixedly arranged on the outer side wall of the storage box, a through hole is formed in one side of the installation frame, a heat dissipation fan is fixedly connected in the installation frame, and an oxygen detector and a carbon dioxide detector are respectively and fixedly connected to the two sides of the installation frame, so that the components of gas in the storage environment in the storage tank can be accurately detected, and the living environment of an internal sample is ensured.

As a preferred technical scheme of the invention, a valve slot is formed in the storage box at one side of the storage slot, a heat dissipation channel is formed at one side of the valve slot, an elastic part is fixedly connected to one side in the valve slot, a piston block is fixedly connected to one side of the elastic part, the piston block is connected with the valve slot in a sliding manner, and the heat dissipation channel is communicated with the mounting frame.

As a preferable technical scheme of the invention, a first through groove and a second through groove are respectively formed in the storage tanks at two sides of the heat dissipation channel, the first through groove and the second through groove are respectively communicated with the oxygen detector and the carbon dioxide detector, and one sides of the first through groove and the second through groove are communicated with the storage tank.

As a preferable technical scheme of the invention, the side wall of the bearing roller is rotatably connected with a ball, and the ball is arranged with the inner wall of the storage tank in a sliding way.

The invention has the following advantages: when the device is used, a cell sample needing to be stored is placed in a test tube, then a sealing sleeve is fixedly sleeved at the port part of the test tube, then the test tube is placed in a limiting groove, the test tube is positioned under the action of a rubber sleeve, then a sealing cover is rotated to drive a bearing roller to rotate, a roller wheel drives a valve block to move downwards under the limiting action of a storage groove along with the rotation, the valve block moves downwards to separate a gas guide groove from a communicating groove, the test tube is completely and hermetically stored, in the long-distance transportation process, an operator can rotate the bearing roller at any time to enable the roller wheel to be placed in a protruding groove, the test tube is reopened, gas components in the test tube are respectively sent into an oxygen detector and a carbon dioxide detector through a first through groove and a second through groove to detect corresponding gas component contents, when the gas components are lower than a standard value, the operator can open an oxygen tank and a carbon dioxide storage tube, corresponding gas components are fed into the storage tank through the air duct and the delivery pipe under the action of the air heater, the test tube is heated, a standard cultivation environment is further provided for cell organisms in the test tube, the activity of the cell organisms is ensured, the gas components are detected through the oxygen detector and the carbon dioxide detector in the process, the electronic valve can be closed when the gas components reach the standard value, meanwhile, under the action of the piston block, when the temperature in the storage tank is higher, the piston block is driven by air pressure to move to one side, hot air enters the mounting frame through the heat dissipation channel, heat dissipation and cooling in the storage tank are realized under the action of the heat dissipation fan, the temperature in the storage tank is further ensured to be in a proper standard state, the activity of the cell organisms is ensured, and after the standard value is reached, the sealing cover can be rotated to drive the bearing roller to rotate, seal the test tube, preserve, pollution sample when can preventing to take out the test tube, the device operation is used conveniently on the whole, can carry out environmental simulation and monitoring to the cell sample alone, for the more energy saving of this kind of mode of low temperature storage, the simulation storage environment that simultaneously can be better, can be long-time keep cell activity, the practicality is higher.

Drawings

Fig. 1 is a schematic structural diagram of an ecological box body for storing a cell-based biological sample.

Fig. 2 is an enlarged schematic view of a in fig. 1.

Fig. 3 is an enlarged structural diagram of B in fig. 1.

Fig. 4 is a schematic structural diagram of a bearing roller in an ecological box for storing cell-based biological samples.

FIG. 5 is a schematic diagram of the structure of the front side of a storage box in an ecological box for storing a cell-based biological sample.

In the figure: 1. a base plate; 2. a storage box; 3. mounting grooves; 4. a storage tank; 5. an oxygen tank; 6. a hot air blower; 7. a carbon dioxide storage tank; 8. a carrier roller; 9. a gas-guide tube; 10. a transfer tube; 11. a test tube; 12. a first through groove; 13. an electronic valve; 14. a raised groove; 15. installing a frame; 16. an oxygen detector; 17. a top plate; 18. a second through groove; 19. a carbon dioxide detector; 20. a heat dissipation fan; 21. a port; 22. a heat dissipation channel; 23. a valve slot; 24. a piston block; 25. an elastic member; 26. a roller; 27. a valve block; 28. a gas guide groove; 29. sealing sleeves; 30. a communicating groove; 31. a reset member; 32. a limiting groove; 33. a rubber sleeve; 34. a ball bearing; 35. a gasket; 36. a sealing cover; 37. positioning a rod; 38. positioning a groove; 39. a storage mechanism; 40. an air guide assembly; 41. a seal assembly; 42. and (6) detecting the component.

Detailed Description

The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are described herein for the purpose of illustration and explanation and not limitation.

Example 1

Referring to fig. 1-5, an ecological box for storing cell-based biological samples includes a bottom plate 1, and further includes: the storage box 2 is fixedly arranged on one side of the bottom plate 1, a storage groove 4 is formed in the storage box 2, and one side of the storage box 2 is fixedly connected with a top plate 17; set up storage mechanism 39 in holding tank 4, including bearing roller 8, bearing roller 8 and the matching activity of holding tank 4 set up, bearing roller 8 one side sets up spacing groove 32, one side fixed connection rubber sleeve 33 in the spacing groove 32, the activity sets up test tube 11 in the spacing groove 32 of rubber sleeve 33 one side, and seal cover 29 is cup jointed to test tube 11 one end, and intercommunication groove 30 is seted up at seal cover 29 both ends, and seal cover 29 one side fixed connection piece 31 that resets between groove 30, 31 one side fixed connection valve block 27 that resets, and air guide groove 28 is seted up to valve block 27 one end, and air guide groove 28 is worker-shaped structure, valve block 27 and seal cover 29 sliding connection, valve block 27 one end is rotated and is connected gyro wheel 26, set up protruding groove 14 in the storage box 2 of holding tank 4 one side, set up air guide assembly 40 between the protruding groove 14, bearing roller 8 one end sets up sealing component 41, air heater 6 is seted up in the mounting groove 3, air heater 6 and bottom plate 1 fixed connection, fixed set up storage tank 7 and oxygen jar 5 on the bottom plate 1 of air heater 6 both sides respectively, and air heater 6 set up air heater 6, storage assembly 6 sets up the storage environment and storage tank 11 rotation detection, storage chamber 8 sets up the quick rotation detection storage environment, and storage chamber 11 sets up through the quick switching storage chamber 8, and the storage chamber 8 rotation detection storage chamber side wall.

When actually carrying out the use, the cell sample that will carry out the storage is placed at test tube 11, then with the fixed port position that cup joints at test tube 11 of seal cover 29, place test tube 11 at spacing inslot 32 afterwards, realize the location to test tube 11 under the effect of rubber sleeve 33, then rotate bearing roller 8, gyro wheel 26 follows to rotate and drives valve block 27 and move down under the limiting displacement of holding tank 4, valve block 27 moves down air guide groove 28 and the separation of intercommunication groove 30, the realization is stored the totally enclosed of test tube 11.

Example 2

Referring to fig. 1-5, the other contents of the present embodiment are the same as embodiment 1, except that: the air guide assembly 40 comprises an air guide pipe 9 fixedly arranged in the storage tank 2, two sides of the air guide pipe 9 are fixedly connected with transfer pipes 10, one end of each transfer pipe 10 is fixedly connected with an electronic valve 13, and the air outlet end of each electronic valve 13 is communicated with the convex groove 14.

The sealing assembly 41 comprises a sealing cover 36 fixedly arranged on one side of the bearing roller 8, a sealing gasket 35 is fixedly arranged on the inner side of the sealing cover 36, a positioning rod 37 is arranged at one end of the sealing cover 36 in a threaded penetrating mode, a positioning groove 38 is formed in the side wall of the storage box 2 on one side of the storage tank 4, the positioning groove 38 is arranged in a matching mode with the positioning rod 37, and the positioning of the bearing roller 8 can be achieved through matching of the positioning rod 37 and the positioning groove 38 during actual storage.

The detection assembly 42 comprises a mounting frame 15 fixedly arranged on the outer side wall of the storage box 2, a through hole 21 is formed in one side of the mounting frame 15, a heat dissipation fan 20 is fixedly connected in the mounting frame 15, and an oxygen detector 16 and a carbon dioxide detector 19 are fixedly connected to two sides of the mounting frame 15 respectively.

A valve groove 23 is formed in the storage box 2 on one side of the storage groove 4, a heat dissipation channel 22 is formed on one side of the valve groove 23, an elastic part 25 is fixedly connected to one side of the valve groove 23, a piston block 24 is fixedly connected to one side of the elastic part 25, the piston block 24 is slidably connected with the valve groove 23, the heat dissipation channel 22 is communicated with the mounting frame 15, and the elastic part 25 can be a spring with telescopic property or an elastic metal sheet and the like.

A first through groove 12 and a second through groove 18 are formed in the storage boxes 2 on two sides of the heat dissipation channel 22 respectively, the first through groove 12 and the second through groove 18 are communicated with the oxygen detector 16 and the carbon dioxide detector 19 respectively, one sides of the first through groove 12 and the second through groove 18 are communicated with the storage tank 4, the side wall of the bearing roller 8 is rotatably connected with a plurality of balls 34, and the balls 34 are arranged in a sliding mode with the inner wall of the storage tank 4.

In the long distance transportation, operating personnel can rotate at any time and bear roller 8, make gyro wheel 26 arrange in protruding groove 14, test tube 11 reopens, the gas composition in test tube 11 advances respectively through first logical groove 12 and second logical groove 18 and carries out the gas composition content that detects the correspondence in oxygen detector 16 and the carbon dioxide detector 19, when being less than standard numerical value, operating personnel can open oxygen jar 5 and carbon dioxide storage tube, send corresponding gas composition into holding tank 4 through air duct 9 and transfer pipe 10 under the effect of air heater 6, and carry out heat treatment to test tube 11, and then provide a standard cultivation environment for the cell biology in the test tube 11, guarantee the biological activity of cell.

Detect gaseous composition through oxygen detector 16 and carbon dioxide detector 19 at this in-process, can close electronic valve 13 after it reaches the standard value, simultaneously under piston block 24's effect, when the temperature in holding tank 4 is higher, atmospheric pressure can drive piston block 24 and remove to one side, steam passes through heat dissipation channel 22 and gets into in the installing frame 15, under the effect of heat dissipation fan 20, the realization is to the heat dissipation cooling in holding tank 4, and then guarantee that the temperature in holding tank 4 is in suitable standard condition, guarantee the biological activity of cell, after reaching the standard value, can rotate sealed lid 36 and drive carrier roller 8 and rotate, seal test tube 11, preserve, can prevent to take out the contaminated sample when test tube 11.

The device operation is used conveniently overall, can carry out environmental simulation and monitoring to the cell sample alone, for the more energy saving of this kind of mode of low temperature storage, simulation storage environment that simultaneously can be better, can be long-time keep cell activity, the practicality is higher.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described above, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides an ecological box is used in biological sample storage based on cell, includes the bottom plate, its characterized in that still includes:

the storage box is arranged on one side of the bottom plate, a storage groove is formed in the storage box, and one side of the storage box is fixedly connected with the top plate;

the storage mechanism comprises a bearing roller, wherein a limiting groove is formed in one side of the bearing roller, a rubber sleeve is fixedly connected to one side of the limiting groove, a test tube is arranged in the limiting groove in one side of the rubber sleeve, a sealing sleeve is sleeved at one end of the test tube, communicating grooves are formed in two ends of the sealing sleeve, a resetting piece is fixedly connected to one side of the sealing sleeve between the communicating grooves, a valve block is fixedly connected to one side of the resetting piece, one end of the valve block is provided with an air guide groove, the air guide groove is of an I-shaped structure, the valve block is connected with the sealing sleeve in a sliding mode, one end of the valve block is rotated to connect a roller, a raised groove is formed in a storage box in one side of the storage groove, an air guide assembly is arranged between the raised grooves, one end of the bearing roller is provided with a sealing assembly, a mounting groove is formed in one end of the storage box, an air heater is arranged in the mounting groove, two sides of the air heater are respectively provided with a carbon dioxide storage tank and an oxygen tank, the air guide tank are communicated with the air heater, a detection assembly is arranged on the side wall of the storage box, the storage environment, the bearing roller is rotated to drive the test tube to rotate, the test tube, the storage environment, the test can automatically realize quick conversion between the environmental simulation and the sealed storage mode, and realize the independent environmental simulation test of the independent environmental environment of a plurality of the storage tanks, and the samples in the storage tanks without mutual influence of the storage tanks.

2. The ecological box for storing the cell-based biological samples according to claim 1, wherein the air guide assembly comprises an air guide tube fixedly arranged in the storage box, two sides of the air guide tube are fixedly connected with a transfer tube, one end of the transfer tube is fixedly connected with the electronic valve, and the air outlet end of the electronic valve is communicated with the protruding groove.

3. The ecological box for storing cell-based biological samples, according to claim 1, characterized in that the sealing assembly comprises a sealing cover disposed on one side of the bearing roller, a sealing gasket is disposed inside the sealing cover, one end of the sealing cover is threaded to penetrate a positioning rod, a sidewall of the storage box on one side of the storage tank is provided with a positioning groove, and the positioning groove is matched with the positioning rod.

4. The ecological box for storing the biological samples based on the cells as claimed in claim 1, wherein the detecting component comprises a mounting frame fixedly arranged on the outer side wall of the storage box, a through hole is formed in one side of the mounting frame, a heat dissipating fan is fixedly connected in the mounting frame, and an oxygen detector and a carbon dioxide detector are respectively and fixedly connected to two sides of the mounting frame.

5. The ecological box for storing cell-based biological samples according to claim 4, wherein a valve slot is formed in the storage box at one side of the storage tank, a heat dissipation channel is formed at one side of the valve slot, an elastic member is fixedly connected to one side of the valve slot, a piston block is fixedly connected to one side of the elastic member, and the heat dissipation channel is communicated with the installation frame.

6. The ecological box for storing cell-based biological samples according to claim 5, wherein the storage boxes at two sides of the heat dissipation channel are respectively provided with a first through groove and a second through groove, and the first through groove and the second through groove are respectively communicated with the oxygen detector and the carbon dioxide detector.

7. The ecological box for storing biological samples based on cells of claim 1, wherein the side wall of the bearing roller is rotatably connected with a ball, and the ball is arranged in a sliding way with the inner wall of the storage groove.