CN112772631A - Based on ecological case is used in biological sample storage of cell - Google Patents

Based on ecological case is used in biological sample storage of cell Download PDF

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Publication number
CN112772631A
CN112772631A CN202011619366.9A CN202011619366A CN112772631A CN 112772631 A CN112772631 A CN 112772631A CN 202011619366 A CN202011619366 A CN 202011619366A CN 112772631 A CN112772631 A CN 112772631A
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China
Prior art keywords
box
fixedly connected
carbon dioxide
casing
ecological
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Pending
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CN202011619366.9A
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Chinese (zh)
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吴海涛
王西俊
程顺巧
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Wuhan Biobank Biotechnology Co ltd
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Wuhan Biobank Biotechnology Co ltd
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Priority to CN202011619366.9A priority Critical patent/CN112772631A/en
Publication of CN112772631A publication Critical patent/CN112772631A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0236Mechanical aspects
    • A01N1/0263Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
    • A01N1/0273Transport containers

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Mechanical Engineering (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses an ecological box for storing biological samples based on cells, which comprises a shell, wherein a heat-insulating lining is adhered to the inner surface of the shell, a storage box is arranged in the shell, and an environment simulation box for adjusting the internal environment of the storage box is fixedly connected to the top of the shell. This ecological box is used based on storage of cell biological sample, environmental simulation case can be for the bin inner member go out with the similar environment of cell biological sample sampling department, and bin self possesses good heat preservation effect, can keep the stability of its inner environment, make cell biological sample can normally preserve in it, be convenient for study sample's the growth condition, and when reagent pipe is taken out to needs, the sealed of reagent pipe can be realized to the mechanism of putting by utilizing getting, and can take out required sample alone, influence to all the other samples is less, and can not too destroy its interior stable environment, it is better to preserve the effect, and the device portable transportation, high durability and convenient use.

Description

Based on ecological case is used in biological sample storage of cell
Technical Field
The invention relates to the technical field of ecological boxes, in particular to an ecological box for storing biological samples based on cells.
Background
Cellular organisms are understood to be all organisms having a cellular structure. The genetic material of a cell organism is DNA, and RNA is the DNA, and has 5 bases and 8 nucleotides. Cellular organisms are divided into two groups according to whether the cells that make up the organism are nuclei coated with or without nuclear membranes: prokaryotes consisting of prokaryotic cells and eukaryotes consisting of eukaryotic cells (not a taxonomic method of classification).
The conventional cell biological tissue samples generally need to be stored at low temperature after being sampled, but need to be cultured in nutrient solution when the research of growth rules and the like is needed, the experiments are carried out in laboratories at present, the low-temperature refrigeration is still troublesome in the transportation process, and the nutrient solution is simply provided for sealed storage when the low-temperature refrigeration is not carried out under the condition, the original growth environment of the samples cannot be simulated, and the vitality or the reduction and even the complete death of the samples in the transfer process influence the research.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides an ecological box for storing cell biological samples, which solves the problems that the cell biological tissue samples need to be refrigerated at low temperature after being sampled, and when the low-temperature refrigeration is carried out without condition, nutrient solution is simply provided for sealing storage, and the activity or reduction and even complete death of the samples influence the research in the transferring process.
In order to achieve the purpose, the invention is realized by the following technical scheme: an ecological box for storing biological samples based on cells comprises a shell, wherein a heat-insulating lining is adhered to the inner surface of the shell, the inside of the shell is provided with a storage box, the top of the shell is fixedly connected with an environment simulation box for adjusting the internal environment of the storage box, the storage box comprises an inner box and an outer box, a heat preservation cavity is arranged between the inner box and the outer box, vent grooves are arranged on four sides of the inner box, an upper positioning plate, a lower positioning plate and a spongy cushion are fixedly connected inside the inner box from top to bottom in sequence, a plurality of jacks are uniformly arranged inside the upper positioning plate and the lower positioning plate, a reagent tube is inserted in the jack, a pick-and-place mechanism is arranged at the top end of the reagent tube, a sealing cover is clamped at the top of the shell, the top end of the taking and placing mechanism penetrates through the sealing cover, and the inner surface of the sealing cover is fixedly connected with a sealing rubber sleeve which is sleeved on the surface of the taking and placing mechanism.
The device comprises a reagent tube and a fetching and placing mechanism, wherein the fetching and placing mechanism comprises a sleeve sleeved on the top end of the reagent tube, a through hole is formed in the lower half part of the side surface of the sleeve, a sealing plug is slidably connected to the inner part of the fetching and placing mechanism and the upper part of the through hole, a bulge matched with the inner diameter of the reagent tube is arranged at the bottom of the sealing plug, and a pull handle is fixedly connected to the top of the sealing.
Preferably, the environmental simulation case includes the under casing, the inside fixedly connected with of under casing is from carbon dioxide gas pitcher and the oxygen jar of taking the air pump, the inside of under casing just is located fixedly connected with cartridge heater between carbon dioxide gas pitcher and the oxygen jar, and the bottom of cartridge heater both sides is passed through the trachea and is communicate with the gas outlet of carbon dioxide gas pitcher and oxygen jar respectively.
Preferably, the upside fixedly connected with infrared electric heating jacket of cartridge heater surface, the top intercommunication of cartridge heater has the gas tube, and the fixed surface of gas tube is connected with the solenoid valve, the top of gas tube runs through the bottom of top, casing and the outer container of under casing in proper order and extends to the heat preservation intracavity.
Preferably, the fan penetrates through the middle of the bottom of the inner box and is fixedly connected with the bottom of the outer box, and a space is reserved between the bottom of the fan and the bottom of the outer box.
Preferably, the outer box and the top on the left side of the shell are fixedly connected with a pressure relief pipe in a penetrating mode, the left end inside the pressure relief pipe is connected with a rubber rod in a sliding mode, and the right end inside the pressure relief pipe is filled with a filter cotton core.
Preferably, fixedly connected with control panel runs through in the left side of casing, fixedly connected with detection device runs through in the left side of outer container, and control panel's right side passes through the wire respectively with detection device, solenoid valve and carbon dioxide gas pitcher and the inside air pump electric connection of oxygen jar.
Preferably, the control panel is provided with a display screen, a digital circuit board and a key panel, the output end of the key panel is electrically connected with the input end of the digital circuit board, and the output end of the digital circuit board is electrically connected with the input end of the display screen.
Preferably, detection device is including installing temperature sensor, oxygen concentration sensor and the carbon dioxide concentration sensor on the base plate, and temperature sensor, oxygen concentration sensor and carbon dioxide concentration sensor's output all with digital circuit board's input electric connection.
Advantageous effects
The invention provides an ecological box for storing a cell-based biological sample. Compared with the prior art, the method has the following beneficial effects:
(1) the ecological box for storing the biological samples based on the cells comprises an environmental simulation box fixedly connected to the top of a shell and used for adjusting the internal environment of the storage box, wherein the storage box comprises an inner box and an outer box, a heat preservation cavity is reserved between the inner box and the outer box, vent grooves are formed in the four sides of the inner box, an upper positioning plate, a lower positioning plate and a sponge pad are fixedly connected to the inside of the inner box from top to bottom in sequence, a plurality of jacks are uniformly formed in the upper positioning plate and the lower positioning plate, reagent tubes are inserted into the jacks, a taking and placing mechanism is arranged at the top ends of the reagent tubes, a sealing cover is clamped at the top of the shell, the top ends of the taking and placing mechanism penetrate through the sealing cover, and the sealing rubber sleeve is fixedly connected to the inner surface of; the taking and placing mechanism comprises a sleeve sleeved at the top end of the reagent tube, the lower half part of the side surface of the sleeve is provided with a through hole, a sealing plug is slidably connected inside the taking and placing mechanism and above the through hole, the bottom of the sealing plug is provided with a bulge matched with the inner diameter of the reagent tube, the top of the sealing plug is fixedly connected with a pull handle, the environment simulation box can form an environment similar to a cell biological sample sampling position for the inner member of the storage box, the storage box has a good heat preservation effect and can keep the stability of the inner environment, so that the cell biological sample can be normally stored in the storage box, the growth condition of the sample can be conveniently researched, when the reagent tube needs to be taken out, the reagent tube can be taken out in a sealing manner by using the taking and placing mechanism, the required sample can be taken out independently, the influence on other samples is small, the stable environment in the storage box cannot be excessively, is convenient to use.
(2) The ecological box for storing the cell-based biological samples comprises a bottom box, wherein a carbon dioxide gas tank and an oxygen tank with an air pump are fixedly connected inside the bottom box, a heating cylinder is fixedly connected inside the bottom box and positioned between the carbon dioxide gas tank and the oxygen tank, the bottoms of two sides of the heating cylinder are respectively communicated with the air outlets of the carbon dioxide gas tank and the oxygen tank through air pipes, an infrared electric heating sleeve is fixedly connected to the upper side of the outer surface of the heating cylinder, an inflation pipe is communicated with the top of the heating cylinder, an electromagnetic valve is fixedly connected to the surface of the inflation pipe, the top end of the inflation pipe sequentially penetrates through the tops of the bottom box, the shell and the outer box and extends into a heat preservation cavity, a fan is fixedly connected in the middle of the bottom of the inner box in a penetrating manner, a distance is reserved between the bottom of the fan and the bottom of the outer box, and a certain amount of oxygen, the gas can be heated to keep the temperature in the storage box stable, the original growth environment of the cell biological sample can be simulated as much as possible, the gas is heated in a non-contact way, and the fan is used for enabling the gas to flow, so that the temperature and the concentration distribution of the gas are more uniform.
(3) The ecological box for storing the cell-based biological samples is characterized in that a control panel is fixedly connected to the left side of a shell in a penetrating manner, a detection device is fixedly connected to the left side of an outer box in a penetrating manner, the right side of the control panel is electrically connected with the detection device, an electromagnetic valve, a carbon dioxide gas tank and an air pump in the oxygen tank through wires respectively, a display screen, a digital circuit board and a key panel are arranged on the control panel, the output end of the key panel is electrically connected with the input end of the digital circuit board, and the output end of the digital circuit board is electrically connected with the input end of; the detection device comprises a temperature sensor, an oxygen concentration sensor and a carbon dioxide concentration sensor which are arranged on the substrate, and the output ends of the temperature sensor, the oxygen concentration sensor and the carbon dioxide concentration sensor are electrically connected with the input end of the digital circuit board; but through setting up environmental parameters such as the required gas strength of manual input and temperature of control panel, detection device detects the concentration and the air temperature of oxygen and carbon dioxide in the bin in real time, and the nimble regulation and control of being convenient for is supplied with, need not artifical timing control oxygen and carbon dioxide's input, but the stability of automatic operation maintenance simulation environment.
Drawings
FIG. 1 is a schematic external view of the present invention;
FIG. 2 is a cross-sectional view of the present invention;
FIG. 3 is a top view of the upper positioning plate of the present invention;
FIG. 4 is a schematic view of the connection between the reagent vessel and the pick-and-place mechanism according to the present invention;
FIG. 5 is a cross-sectional view of the seal cap of the present invention;
FIG. 6 is a cross-sectional view of the pressure relief tube of the present invention;
FIG. 7 is a block diagram of the control panel and the detecting device according to the present invention.
In the figure: 1-shell, 2-storage box, 21-inner box, 22-outer box, 23-air groove, 24-upper positioning plate, 25-lower positioning plate, 26-sponge cushion, 27-jack, 3-environment simulation box, 31-bottom box, 32-carbon dioxide gas tank, 33-oxygen tank, 34-heating cylinder, 35-air pipe, 36-infrared electric heating sleeve, 37-inflation pipe, 38-electromagnetic valve, 39-fan, 4-reagent pipe, 5-taking and placing mechanism, 51-sleeve pipe, 52-through hole, 53-sealing plug, 54-pull handle, 6-sealing cover, 7-sealing rubber sleeve, 8-pressure relief pipe, 81-rubber rod, 82-filtering cotton core, 9-control panel, 91-display screen, 92-digital circuit board, 93-key panel, 10-detection device, 101-temperature sensor, 102-oxygen concentration sensor, 103-carbon dioxide concentration sensor and 11-heat preservation lining.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-7, the present invention provides a technical solution: an ecological box for storing biological samples based on cells comprises a shell 1, wherein a heat-insulating lining 11 is adhered to the inner surface of the shell 1, a storage box 2 is arranged inside the shell 1, an environment simulation box 3 for adjusting the internal environment of the storage box 2 is fixedly connected to the top of the shell 1, the storage box 2 comprises an inner box 21 and an outer box 22, a heat preservation cavity is arranged between the inner box 21 and the outer box 22, the four sides of the inner box 21 are all provided with vent grooves 23, the inner part of the inner box 21 is fixedly connected with an upper positioning plate 24, a lower positioning plate 25 and a spongy cushion 26 from top to bottom, the inner parts of the upper positioning plate 24 and the lower positioning plate 25 are all evenly provided with a plurality of jacks 27, the reagent tube 4 is inserted into the jack 27, the picking and placing mechanism 5 is arranged at the top end of the reagent tube 4, the sealing cover 6 is clamped at the top of the shell 1, the top end of the picking and placing mechanism 5 penetrates through the sealing cover 6, and the inner surface of the sealing cover 6 is fixedly connected with a sealing rubber sleeve 7 which is sleeved on the surface of the taking and placing mechanism 5.
The taking and placing mechanism 5 comprises a sleeve 51 sleeved at the top end of the reagent tube 4, the lower half part of the side surface of the sleeve 51 is provided with a through hole 52, a sealing plug 53 is slidably connected inside the taking and placing mechanism 5 and positioned above the through hole 52, the bottom of the sealing plug 53 is provided with a bulge matched with the inner diameter of the reagent tube 4, the top of the sealing plug 53 is fixedly connected with a pull handle 54, the environment simulation box 3 can form an environment similar to the cell biological sample sampling position for the internal components of the storage box 2, the storage box 2 has good heat preservation effect and can keep the stability of the internal environment, so that the cell biological sample can be normally stored in the environment, the growth condition of the sample can be conveniently researched, when the reagent tube 4 needs to be taken out, the reagent tube 4 can be taken out in a sealed mode by using the taking and placing mechanism 5, the required sample can be taken out independently, the influence on other samples is small, the preservation effect is better, and the device portable transportation, convenient to use.
The environment simulation box 3 comprises a bottom box 31, a carbon dioxide gas tank 32 and an oxygen tank 33 with gas pumps are fixedly connected inside the bottom box 31, a heating cylinder 34 is fixedly connected inside the bottom box 31 and positioned between the carbon dioxide gas tank 32 and the oxygen tank 33, bottoms of two sides of the heating cylinder 34 are respectively communicated with gas outlets of the carbon dioxide gas tank 32 and the oxygen tank 33 through gas pipes 35, an infrared electric heating sleeve 36 is fixedly connected to the upper side of the outer surface of the heating cylinder 34, the top of the heating cylinder 34 is communicated with an inflation pipe 37, an electromagnetic valve 38 is fixedly connected to the surface of the inflation pipe 37, the top end of the inflation pipe 37 sequentially penetrates through the top of the bottom box 31, bottoms of the shell 1 and the outer box 22 and extends into a heat preservation cavity, the environment simulation box 3 can fill a certain amount of oxygen or carbon dioxide into the storage box 2 and can heat the gas to keep the temperature in the storage box 2 stable, and can simulate the original growth environment of a cell, the gas is heated in a non-contact mode, the fan 39 is used for enabling the gas to flow, the temperature and the concentration distribution of the gas can be more uniform, the fan 39 penetrates through and is fixedly connected with the middle of the bottom of the inner box 21, a distance is reserved between the bottom of the fan 39 and the bottom of the outer box 22, the outer box 22 and the top of the left side of the shell 1 penetrate and is fixedly connected with the pressure relief pipe 8, the left end inside the pressure relief pipe 8 is connected with a rubber rod 81 in a sliding mode, and the right end inside the pressure relief pipe 8 is filled with the filter cotton core.
A control panel 9 is fixedly connected to the left side of the casing 1 in a penetrating manner, a detection device 10 is fixedly connected to the left side of the outer box 22 in a penetrating manner, the right side of the control panel 9 is electrically connected with the detection device 10, the electromagnetic valve 38, the carbon dioxide gas tank 32 and the air pump inside the oxygen tank 33 through conducting wires, a display screen 91, a digital circuit board 92 and a key panel 93 are arranged on the control panel 9, the output end of the key panel 93 is electrically connected with the input end of the digital circuit board 92, the output end of the digital circuit board 92 is electrically connected with the input end of the display screen 91, the detection device 10 comprises a temperature sensor 101, an oxygen concentration sensor 102 and a carbon dioxide concentration sensor 103 which are arranged on a substrate, the temperature sensor 101 is a D6T-44L-06/06H type temperature sensor, the oxygen concentration sensor 10201-ES1-, the carbon dioxide concentration sensor 103 is an MH-410D type carbon dioxide concentration sensor, and the output ends of the temperature sensor 101, the oxygen concentration sensor 102 and the carbon dioxide concentration sensor 103 are electrically connected with the input end of the digital circuit board 92; but through setting up environmental parameters such as the required gas strength of manual input and temperature of control panel 9, detection device 10 real-time detection bin 2 internal oxygen and carbon dioxide's concentration and air temperature, the nimble regulation and control of being convenient for is supplied with, need not artifical timing control oxygen and carbon dioxide's input, but the stability of simulation environment is maintained in automatic operation.
Meanwhile, the contents which are not described in detail in the specification belong to the prior art which is known by the person skilled in the art, and the model parameters of each electric appliance are not particularly limited, and conventional equipment can be used.
When the device is used, the reagent tube 4 filled with nutrient solution and samples is inserted into the sleeve 51, the sealing plug 53 is plugged into the sleeve 51, then the sealing plug passes through the sealing cover 6 and is inserted into the storage box 2, the reagent tube 4 is inserted into the insertion holes 27 of the upper positioning plate 24 and the lower positioning plate 25, and the storage box 2 can be sealed after all the samples are placed; after detecting parameters such as oxygen concentration, carbon dioxide concentration and temperature, etc. at the sampling position, the parameters are input into the control panel 9, meanwhile, the temperature sensor 101, the oxygen concentration sensor 102 and the carbon dioxide concentration sensor 103 on the detection device 10 detect three kinds of data, after the data comparison is carried out by the digital circuit board 92, controlling the gas pump in the carbon dioxide gas tank 32 or the oxygen gas tank 33 to be opened, discharging a certain amount of gas into the heating cylinder 34, then the infrared electric heating jacket 36 is started to heat the gas, when the gas is heated to a certain temperature, the electromagnetic valve 38 is opened to discharge the gas into the storage box 2, at the moment, the glue stick 81 needs to be pulled out to discharge the original air in the storage box 2 through the pressure relief pipe 8, stopping gas supply when the value detected by the detection device 10 reaches a preset value, detecting the value in real time at the later stage, and starting the gas pump again to supply the seed gas when the value of a certain gas is lower than the preset value; in the uniform speed process, the fan 39 is started in real time, and air on the outer layer of the inner box 21 is extracted and exhausted to the inner layer, so that the air flow circulation is promoted, and the gas concentration and the temperature are kept balanced.
After the sample needs to be taken out, the sealing plug 53 is pressed down through the pull handle 54 to plug the top opening of the reagent tube 4, then the taking and placing mechanism 5 and the reagent tube 4 are pulled out, and then the top opening of the sealing cover 6 is plugged by another rubber plug quickly.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (8)

1. The utility model provides an ecological box for storing based on cell biological sample, includes casing (1), and the internal surface paste of casing (1) has heat preservation inside lining (11), the inside of casing (1) is provided with bin (2), the environmental simulation case (3) of the top fixedly connected with adjustment bin (2) internal environment of casing (1), its characterized in that: the storage box (2) comprises an inner box (21) and an outer box (22), a heat preservation cavity is reserved between the inner box (21) and the outer box (22), the four sides of the inner box (21) are all provided with vent grooves (23), the inner part of the inner box (21) is fixedly connected with an upper positioning plate (24), a lower positioning plate (25) and a spongy cushion (26) from top to bottom in sequence, a plurality of jacks (27) are uniformly arranged in the upper positioning plate (24) and the lower positioning plate (25), a reagent tube (4) is inserted in the jack (27), a pick-and-place mechanism (5) is arranged at the top end of the reagent tube (4), the top of the shell (1) is clamped with a sealing cover (6), the top end of the taking and placing mechanism (5) penetrates through the sealing cover (6), and the inner surface of the sealing cover (6) is fixedly connected with a sealing rubber sleeve (7) which is sleeved on the surface of the pick-and-place mechanism (5);
get and put mechanism (5) including the cover establish sleeve pipe (51) on reagent pipe (4) top, and the latter half of sleeve pipe (51) side seted up through-hole (52), the inside of getting put mechanism (5) and the top sliding connection who is located through-hole (52) has sealing plug (53), and the bottom of sealing plug (53) is provided with the arch with reagent pipe (4) internal diameter looks adaptation, the top fixedly connected with pull handle (54) of sealing plug (53).
2. The ecological box for storing cell-based biological samples according to claim 1, wherein: environmental simulation case (3) include under casing (31), the inside fixedly connected with of under casing (31) is from carbon dioxide gas pitcher (32) and oxygen cylinder (33) of taking the air pump, the inside of under casing (31) just is located fixedly connected with cartridge heater (34) between carbon dioxide gas pitcher (32) and oxygen cylinder (33), and the bottom of cartridge heater (34) both sides is passed through trachea (35) and is communicate with the gas outlet of carbon dioxide gas pitcher (32) and oxygen cylinder (33) respectively.
3. The ecological box for storing cell-based biological samples according to claim 2, characterized in that: the utility model discloses a heat preservation box, including heating cylinder (34), the upside fixedly connected with infrared electric heating jacket (36) of heating cylinder (34) surface, the top intercommunication of heating cylinder (34) has gas tube (37), and the fixed surface of gas tube (37) is connected with solenoid valve (38), the top of gas tube (37) runs through the bottom of top, casing (1) and outer container (22) of under casing (31) in proper order and extends to the heat preservation intracavity.
4. The ecological box for storing cell-based biological samples according to claim 1, wherein: the middle of the bottom of the inner box (21) is fixedly connected with a fan (39) in a penetrating mode, and a space is reserved between the bottom of the fan (39) and the bottom of the outer box (22).
5. The ecological box for storing cell-based biological samples according to claim 1, wherein: fixedly connected with pressure release pipe (8) is run through with the left top of casing (1) in outer container (22), the inside left end sliding connection of pressure release pipe (8) has gluey stick (81), the inside right-hand member packing of pressure release pipe (8) has filtering cotton core (82).
6. The ecological box for storing cell-based biological samples according to claim 1, wherein: fixedly connected with control panel (9) runs through in the left side of casing (1), fixedly connected with detection device (10) runs through in the left side of outer container (22), and the right side of control panel (9) is passed through the wire and is respectively with detection device (10), solenoid valve (38) and carbon dioxide gas pitcher (32) and oxygen jar (33) inside air pump electric connection.
7. The ecological box for storing cell-based biological samples according to claim 6, wherein: the control panel (9) is provided with a display screen (91), a digital circuit board (92) and a key panel (93), the output end of the key panel (93) is electrically connected with the input end of the digital circuit board (92), and the output end of the digital circuit board (92) is electrically connected with the input end of the display screen (91).
8. The ecological box for storing cell-based biological samples according to claim 6, wherein: the detection device (10) comprises a temperature sensor (101), an oxygen concentration sensor (102) and a carbon dioxide concentration sensor (103) which are arranged on a substrate, and the output ends of the temperature sensor (101), the oxygen concentration sensor (102) and the carbon dioxide concentration sensor (103) are electrically connected with the input end of the digital circuit board (92).
CN202011619366.9A 2020-12-30 2020-12-30 Based on ecological case is used in biological sample storage of cell Pending CN112772631A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115251047A (en) * 2022-08-24 2022-11-01 曾峰 Based on ecological case is used in biological sample storage of cell

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CN211618590U (en) * 2019-12-30 2020-10-02 上海曼为科技有限公司 Cell sample storage device for biological research
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Publication number Priority date Publication date Assignee Title
CN106185054A (en) * 2016-08-26 2016-12-07 宁芳 A kind of Medical portable cold closet
CN106904356A (en) * 2017-03-01 2017-06-30 中华人民共和国齐齐哈尔出入境检验检疫局 Sampling box for food safety inspection
CN210654515U (en) * 2019-08-06 2020-06-02 杭州必通检测技术服务有限公司 Active carbon sampling pipe heat preservation device
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Publication number Priority date Publication date Assignee Title
CN115251047A (en) * 2022-08-24 2022-11-01 曾峰 Based on ecological case is used in biological sample storage of cell
CN115251047B (en) * 2022-08-24 2023-08-11 曾峰 Ecological box for storing cell-based biological samples

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