CN117683634A - Stem cell culture device and method for diabetes treatment - Google Patents
Stem cell culture device and method for diabetes treatment Download PDFInfo
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- CN117683634A CN117683634A CN202410131298.3A CN202410131298A CN117683634A CN 117683634 A CN117683634 A CN 117683634A CN 202410131298 A CN202410131298 A CN 202410131298A CN 117683634 A CN117683634 A CN 117683634A
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 49
- 206010012601 diabetes mellitus Diseases 0.000 title claims abstract description 26
- 238000004113 cell culture Methods 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000012258 culturing Methods 0.000 claims abstract description 12
- 238000007789 sealing Methods 0.000 claims description 14
- 239000000463 material Substances 0.000 claims description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 239000012535 impurity Substances 0.000 abstract description 7
- 239000000126 substance Substances 0.000 abstract description 5
- 239000003814 drug Substances 0.000 abstract description 3
- 230000011712 cell development Effects 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 210000004504 adult stem cell Anatomy 0.000 description 1
- 230000001363 autoimmune Effects 0.000 description 1
- 210000000227 basophil cell of anterior lobe of hypophysis Anatomy 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 210000004263 induced pluripotent stem cell Anatomy 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 210000004153 islets of langerhan Anatomy 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 238000009168 stem cell therapy Methods 0.000 description 1
- 238000009580 stem-cell therapy Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the technical field of cell culture, in particular to a device and a method for culturing stem cells for treating diabetes, wherein a plurality of test tubes are placed on a placing tray, the placing tray is placed on a supporting frame in a base, a cover plate body and the base are closed, a first cylinder is started to drive a moving rod to move downwards, the supporting tray drives a plurality of suction nozzles to move downwards, test tube plugs at the tops of the test tubes are taken out through the plurality of suction nozzles, the supporting tray is then moved upwards to drive the test tube plugs to be far away from the test tubes, a first screw motor is started to drive a first slider to slide on the base to adjust the position of a second slider, and then the second screw motor is started to drive the second slider to adjust the position, so that a medicine adding tube is moved above the corresponding test tube to add substances required by stem cell culture, and therefore the whole culture process of stem cells is carried out under an environment isolated from the outside, and the influence of entering external impurities on stem cell development is avoided.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a stem cell culture device and a stem cell culture method for treating diabetes.
Background
In the treatment of diabetes, stem cell therapy is a leading-edge technique, and mainly involves the use of stem cells (such as embryonic stem cells, induced pluripotent stem cells or adult stem cells) to differentiate into beta cells capable of secreting insulin, thereby replacing islet cells damaged by autoimmune reaction or islet failure. To achieve this, a device for culturing and differentiating these stem cells is required.
The existing stem cell culture device needs to take out a test tube to detect the culture process of stem cells in the test tube in the culture process, so that the culture device needs to be opened, and external impurities easily enter the culture device.
Disclosure of Invention
The invention aims to provide a device and a method for culturing diabetes mellitus treatment stem cells, which aim to ensure that the whole culturing process of the stem cells is carried out in an environment isolated from the outside.
In order to achieve the above object, in a first aspect, the invention provides a device for culturing stem cells for treating diabetes, which comprises a support assembly, a placement assembly and a feeding assembly, wherein the support assembly comprises a base, a cover plate and a driving cylinder, the cover plate comprises a cover plate body, a first cylinder, a moving rod, a support disc and a plurality of suction nozzles, the cover plate body is in sliding connection with the base and is positioned at the top of the base, the first cylinder is fixed on the cover plate body, the moving rod is fixedly connected with the output end of the first cylinder, the support disc is fixed on the moving rod, the suction nozzles are arranged on the support disc, the placement assembly comprises a placement disc, a support frame and a first spring, the support frame is arranged in the base in a sliding manner, the placement disc is arranged on the support frame, the feeding assembly comprises a first slider, a first screw motor, a second slider, a second screw motor, a dosing tube and a slider, the first screw motor is in sliding connection with the base, the first screw motor is positioned at one side of the first screw motor and is fixedly connected with the output end of the first screw motor and the second screw motor, the second screw motor is connected with the first screw tube and the second screw is fixedly connected with the first screw.
The support assembly further comprises an air adding pump and a filter, wherein the air adding pump is communicated with the base and is located on one side of the base, and the filter is arranged on one side of the air adding pump.
The cover plate further comprises a reset spring, and the reset spring is arranged between the first air cylinder and the supporting disc.
The support assembly further comprises a sealing ring, wherein the sealing ring is fixedly connected with the base and is located between the base and the cover plate body.
The feeding assembly further comprises a pipe taking device, the pipe taking device is arranged on the second sliding block, the supporting assembly further comprises a material taking box, the material taking box comprises a box body, two baffles, a second air cylinder and a box cover, the box body is fixed on one side of the base and communicated with the base, the two baffles are in sliding connection with the box body and located at the communicating position of the box body and the base, the second air cylinder is connected with the baffles, and the box cover is arranged at the top of the box body in a sliding mode.
The material taking box further comprises a sealing gasket, and the sealing gasket is arranged on one side of the box cover.
The placing plate is provided with a positioning groove, the supporting frame is provided with a positioning block, and the positioning block is positioned on one side matched with the positioning groove.
In a second aspect, the present invention also provides a method for using a stem cell culture device for treating diabetes, comprising: taking out the supporting disc, putting the test tube with the tube plug on the supporting disc, and putting the supporting disc on the supporting frame;
starting the first cylinder to drive the supporting disc to move downwards, so that a plurality of suction nozzles can suck out tube plugs on the test tube;
the supporting disc is moved upwards to lift the pipe plug to the top for storage;
and starting the first sliding block and the second sliding block to drive the dosing tube to move so as to add corresponding reagents to the test tube.
According to the device and the method for culturing the stem cells for treating diabetes, the stem cells are placed in the test tubes when the device and the method are used, the placing plate is taken out, so that a plurality of test tubes can be placed on the placing plate, the placing plate is placed back on the supporting frame in the base, the cover plate body and the base are closed, the first cylinder is started to drive the moving rod to move downwards, the supporting plate can drive the plurality of suction nozzles to move downwards, the test tube plugs at the tops of the test tubes can be taken out through the plurality of suction nozzles, the supporting plate is moved upwards to drive the test tube plugs to be far away from the test tubes, the first screw motor is started to drive the first slider to slide on the base to adjust the position of the second slider, the second screw motor is started to drive the second slider to adjust the position, the medicine adding pipe can be moved to the corresponding upper side to add substances required by the stem cells, and therefore the whole stem cell culturing process can be carried out under the condition of isolating the whole stem cells from the environment, and the external impurities affecting the stem cells are prevented from entering the development.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a block diagram of a stem cell culture apparatus for treating diabetes according to a first embodiment of the present invention.
Fig. 2 is a right side construction view of a diabetes treatment stem cell culture apparatus according to a first embodiment of the present invention.
FIG. 3 is a cross-sectional view showing a stem cell culture apparatus for treating diabetes according to a first embodiment of the present invention.
FIG. 4 is a block diagram of a stem cell culture apparatus for treating diabetes according to a second embodiment of the present invention.
FIG. 5 is a cross-sectional view showing a stem cell culture apparatus for treating diabetes according to a second embodiment of the present invention.
Fig. 6 is an enlarged view of a portion of detail a of fig. 5.
FIG. 7 is a flow chart of a method of culturing diabetes-treating stem cells according to a third embodiment of the present invention.
Base 104, apron 105, actuating cylinder 106, apron body 107, first cylinder 108, movable rod 109, supporting disk 110, suction nozzle 111, placing tray 112, support frame 113, first spring 114, first slider 115, first screw motor 116, second slider 117, second screw motor 118, dosing tube 119, solenoid valve 120, air entrainment pump 201, filter 202, reset spring 203, sealing washer 204, box 207, baffle 208, second cylinder 209, lid 210, sealing pad 211, constant head tank 212, locating piece 213, third screw motor 214, third slider 215, electromagnet 216, magnet ring 217.
Detailed Description
Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative and intended to explain the present invention and should not be construed as limiting the invention.
In the description of the present invention, it should be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the present invention and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present invention. Furthermore, in the description of the present invention, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
First embodiment
Referring to FIGS. 1-3, the invention provides a stem cell culture device for treating diabetes, which comprises a supporting component, a placing component and a feeding component, the support assembly comprises a base 104, a cover 105 and a driving cylinder 106, wherein the cover 105 comprises a cover body 107, a first cylinder 108, a moving rod 109, a support plate 110 and a plurality of suction nozzles 111, the cover body 107 is in sliding connection with the base 104, and is located on top of the base 104, the first cylinder 108 is fixed on the cover body 107, the moving rod 109 is fixedly connected with the output end of the first cylinder 108, the supporting plate 110 is fixed on the moving rod 109, the plurality of suction nozzles 111 are arranged on the supporting plate 110, the placement assembly includes a placement tray 112, a support 113, and a first spring 114, the support 113 being slidably disposed within the base 104, the first spring 114 is disposed between the support 113 and the base 104, the placement tray 112 has a plurality of through holes, the placement tray 112 is disposed on the support frame 113, the feeding assembly comprises a first slider 115, a first screw motor 116, a second slider 117, a second screw motor 118, a dosing tube 119 and an electromagnetic valve 120, wherein the first slider 115 is in sliding connection with the base 104, and is located at one side of the support plate 110, the output end of the first screw motor 116 is screw-coupled with the first slider 115, the second slider 117 is slidably connected to the first slider 115, the second screw motor 118 is fixed to the first slider 115, the output end of the second screw motor 118 is in threaded connection with the second slider 117, the dosing tube 119 is fixed on the second slider 117, and the electromagnetic valve 120 is arranged on the dosing tube 119.
In this embodiment, when in use, stem cells are placed in test tubes, then the placing tray 112 is taken out, so that a plurality of test tubes can be placed on the placing tray 112, then the placing tray 112 is put back on the supporting frame 113 in the base 104, then the cover plate 105 plate body and the base 104 are closed, then the first cylinder 108 is started to drive the moving rod 109 to move downwards, so that the supporting tray 110 can drive the suction nozzles 111 to move downwards, the test tube plug at the top of the test tube can be taken out through the suction nozzles 111, then the supporting tray 110 is moved upwards to drive the test tube plug to be far away from the test tube, then the first screw motor 116 is started to drive the first slider 115 to slide on the base 104 to adjust the position of the second slider 117, then the second screw motor 118 is started to drive the second slider 117 to adjust the position, so that the medicine adding tube 119 can be moved above the corresponding test tube to add substances required for stem cell cultivation, thus the whole stem cells can be isolated from the corresponding environment under the base 104, and the environmental conditions can be prevented from being influenced by the environment, and the environmental conditions can be changed.
Second embodiment
Referring to fig. 4 to 6, the present invention further provides a stem cell culture apparatus for treating diabetes, wherein the support assembly further comprises an air pump 201 and a filter 202, the air pump 201 is communicated with the base 104 and is located at one side of the base 104, and the filter 202 is disposed at one side of the air pump 201. In order to keep the space in the base 104 in an impurity-free environment, the air adding pump 201 is further provided, and air entering the base 104 can be filtered by the filter 202 to reduce the entry of impurities, so that the air with impurities in the base 104 can be discharged and filtered air can be introduced to further reduce the influence on stem cell culture.
The cover plate 105 further comprises a return spring 203, the return spring 203 being arranged between the first cylinder 108 and the support plate 110. The supporting plate 110 can be automatically pulled up by the elastic force of the return spring 203, so that the working efficiency can be improved.
The support assembly further comprises a sealing ring 204, wherein the sealing ring 204 is fixedly connected with the base 104 and is located between the base 104 and the cover plate body 107. The sealing ring 204 can increase the tightness between the base 104 and the cover plate body 107, so that the sealing effect is better.
The feeding assembly further comprises a tube taking device, the tube taking device is arranged on the second sliding block 117, the supporting assembly further comprises a material taking box, the material taking box comprises a box body 207, two baffles 208, a second air cylinder 209 and a box cover 210, the box body 207 is fixed on one side of the base 104 and communicated with the base 104, the two baffles 208 are in sliding connection with the box body 207 and located at the communicating position of the box body 207 and the base 104, the second air cylinder 209 is connected with the baffles 208, and the box cover 210 is arranged at the top of the box body 207 in a sliding mode. In order to view the stem cells in a certain test tube in a targeted manner, the application is further provided with the box body 207, so that the test tube can be taken out through the tube taking device, then the test tube is fed into the box body 207 through the cooperation of the first sliding block 115 and the second sliding block 117, then the two baffles 208 are driven to move through the second air cylinder 209 to seal the inlet opening, and then the box cover 210 is opened to sample and test the stem cells in the test tube. Thus, the cover plate 105 does not need to be integrally opened, so that the use is more convenient.
The take out cartridge further includes a gasket 211, and the gasket 211 is disposed at one side of the cap 210. The gasket 211 may increase sealability between the cover 210 and the base 104, so that foreign substances may be prevented from entering.
The placing tray 112 has a positioning groove 212, and the supporting frame 113 has a positioning block 213, and the positioning block 213 is located at a side matching with the positioning groove 212. The positioning groove 212 may be matched with the positioning block 213 to better position the placing tray 112.
The pipe taking device comprises a third screw motor 214, a third sliding block 215 and an electromagnet 216, wherein the third screw motor 214 is fixed on the second sliding block 117, and the third sliding block 215 is in threaded connection with the output end of the third screw motor 214 and is in sliding connection with the second sliding block 117. The placement assembly further includes a plurality of magnet rings 217, the plurality of magnet rings 217 being positioned at a plurality of through holes in the placement tray 112. The third screw motor 214 is started to drive the third slider 215 to move downwards, so that after the electromagnet 216 is electrified, the third slider 215 can be matched with the magnet ring 217 to lift the test tube from the placing disc 112, after the test tube is moved into the box 207, the electromagnet 216 is disconnected to place the test tube into the box 207, after the test tube is sampled, the baffle 208 can be opened again to place the test tube back, and before the baffle 208 is opened, air in a cavity in the box 207 can be discharged by accessing air entering the air pump 201, so that impurities are prevented from entering the base 104.
Third embodiment
Referring to fig. 7, the present invention further provides a method for using a stem cell culture device for treating diabetes, which comprises:
s101, taking out the support plate 110, placing the test tube with the tube plug, and then placing the support plate 110 on the support frame 113;
in use, stem cells are placed in test tubes and then the tray 112 is removed so that a plurality of test tubes can be placed on the tray 112 and then the tray 112 is returned to the support 113 in the base 104.
S102, starting the first cylinder to drive the supporting disc 110 to move downwards, so that a plurality of suction nozzles 111 can suck out tube plugs on the test tube;
and then closing the cover plate 105 and the base 104, starting the first cylinder 108 to drive the moving rod 109 to move downwards, so that the supporting plate 110 can drive the plurality of suction nozzles 111 to move downwards, the test tube plug at the top of the test tube can be taken out through the plurality of suction nozzles 111, and then moving the supporting plate 110 upwards to drive the test tube plug away from the test tube.
S103, moving up the supporting disc 110 to lift the pipe plug to the top for storage;
s104, the first slide block 115 and the second slide block drive the dosing tube 119 to move so as to add corresponding reagents to the test tube.
Then, the first screw motor 116 is started to drive the first slider 115 to slide on the base 104 to adjust the position of the second slider 117, and then the second screw motor 118 is started to drive the second slider 117 to adjust the position, so that the dosing tube 119 can be moved to the position above the corresponding test tube to add substances required by stem cell cultivation, and the whole stem cell cultivation process can be performed in an environment isolated from the outside, so that the influence of the entering of external impurities on stem cell development is avoided.
The above disclosure is only a preferred embodiment of the present invention, and it should be understood that the scope of the invention is not limited thereto, and those skilled in the art will appreciate that all or part of the procedures described above can be performed according to the equivalent changes of the claims, and still fall within the scope of the present invention.
Claims (8)
1. A stem cell culture device for treating diabetes is characterized in that,
including supporting component, placing the subassembly and feeding subassembly, the supporting component includes base, apron and actuating cylinder, the apron includes apron body, first cylinder, carriage release lever, supporting disk and a plurality of suction nozzle, the apron body with base sliding connection is located the top of base, first cylinder is fixed on the apron body, the carriage release lever with the output fixed connection of first cylinder, the supporting disk is fixed on the carriage release lever, a plurality of the suction nozzle sets up on the supporting disk, place the subassembly including placing disk, support frame and first spring, the support frame slides and sets up in the base, first spring sets up the support frame with place the disk has a plurality of through-holes, place the dish setting is in on the support frame, the feeding subassembly includes first slider, first screw motor, second slider, second screw motor, dosing tube and solenoid valve, first slider with be located one side of supporting disk, the output of first screw motor is in with first screw motor, first screw motor and first screw connection are in with the second screw connection is in to the second screw motor the second screw connection is in the dosing tube.
2. A diabetes mellitus treatment stem cell culture apparatus as claimed in claim 1, wherein,
the support assembly further comprises an air adding pump and a filter, the air adding pump is communicated with the base and located on one side of the base, and the filter is arranged on one side of the air adding pump.
3. A diabetes mellitus treatment stem cell culture apparatus as claimed in claim 2, wherein,
the cover plate further comprises a return spring, and the return spring is arranged between the first cylinder and the supporting disc.
4. A device for culturing diabetes mellitus treatment stem cells according to claim 3,
the support assembly further comprises a sealing ring, wherein the sealing ring is fixedly connected with the base and is positioned between the base and the cover plate body.
5. The device for culturing diabetes mellitus treatment stem cells of claim 4,
the feeding assembly further comprises a pipe taking device, the pipe taking device is arranged on the second sliding block, the supporting assembly further comprises a material taking box, the material taking box comprises a box body, two baffles, a second air cylinder and a box cover, the box body is fixed on one side of the base and communicated with the base, the two baffles are in sliding connection with the box body and located at the communicating position of the box body and the base, the second air cylinder is connected with the baffles, and the box cover is arranged at the top of the box body in a sliding mode.
6. A diabetes mellitus treatment stem cell culture apparatus as claimed in claim 5, wherein,
the material taking box further comprises a sealing gasket, and the sealing gasket is arranged on one side of the box cover.
7. The device for culturing diabetes mellitus treatment stem cells of claim 6,
the placing tray is provided with a positioning groove, the supporting frame is provided with a positioning block, and the positioning block is positioned on one side matched with the positioning groove.
8. A method of using a device for culturing stem cells for treating diabetes according to any one of claim 1 to 7, wherein,
comprising the following steps: taking out the supporting disc, putting the test tube with the tube plug on the supporting disc, and putting the supporting disc on the supporting frame;
starting the first cylinder to drive the supporting disc to move downwards, so that a plurality of suction nozzles can suck out tube plugs on the test tube;
the supporting disc is moved upwards to lift the pipe plug to the top for storage;
and starting the first sliding block and the second sliding block to drive the dosing tube to move so as to add corresponding reagents to the test tube.
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|---|---|---|---|
| CN202410131298.3A CN117683634B (en) | 2024-01-31 | 2024-01-31 | Stem cell culture device and method for diabetes treatment |
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| CN202410131298.3A CN117683634B (en) | 2024-01-31 | 2024-01-31 | Stem cell culture device and method for diabetes treatment |
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| CN117683634A true CN117683634A (en) | 2024-03-12 |
| CN117683634B CN117683634B (en) | 2024-05-07 |
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