CN114381371A - A adenocarcinoma stem cell culture apparatus for breast cancer immunotherapy - Google Patents
A adenocarcinoma stem cell culture apparatus for breast cancer immunotherapy Download PDFInfo
- Publication number
- CN114381371A CN114381371A CN202210038165.2A CN202210038165A CN114381371A CN 114381371 A CN114381371 A CN 114381371A CN 202210038165 A CN202210038165 A CN 202210038165A CN 114381371 A CN114381371 A CN 114381371A
- Authority
- CN
- China
- Prior art keywords
- cleaning
- culture
- culture device
- driving motor
- cleaning device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 21
- 208000009956 adenocarcinoma Diseases 0.000 title claims abstract description 13
- 238000004113 cell culture Methods 0.000 title claims abstract description 13
- 206010006187 Breast cancer Diseases 0.000 title claims abstract description 10
- 208000026310 Breast neoplasm Diseases 0.000 title claims abstract description 10
- 238000002619 cancer immunotherapy Methods 0.000 title claims abstract description 10
- 238000004140 cleaning Methods 0.000 claims abstract description 89
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 61
- 230000007306 turnover Effects 0.000 claims abstract description 11
- 230000007246 mechanism Effects 0.000 claims description 22
- 238000005096 rolling process Methods 0.000 claims description 16
- 230000005540 biological transmission Effects 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- 238000011084 recovery Methods 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 8
- 239000007921 spray Substances 0.000 claims description 7
- 238000005507 spraying Methods 0.000 claims description 4
- 230000000149 penetrating effect Effects 0.000 claims 1
- 239000002699 waste material Substances 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract description 3
- 238000005406 washing Methods 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004851 dishwashing Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000002070 germicidal effect Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/20—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought
- B08B9/28—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought the apparatus cleaning by splash, spray, or jet application, with or without soaking
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/20—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought
- B08B9/36—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought the apparatus cleaning by using brushes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Clinical Laboratory Science (AREA)
- Analytical Chemistry (AREA)
- Mechanical Engineering (AREA)
- Molecular Biology (AREA)
- Computer Hardware Design (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to the technical field of stem cell culture, and particularly discloses an adenocarcinoma stem cell culture device for breast cancer immunotherapy, which comprises a culture device whole body, a cleaning device and a turnover cover, wherein a display screen is embedded in the top of the right front left side of the culture device whole body, a control panel is embedded in the bottom of the right front left side of the culture device whole body, a suction door is movably arranged in the middle top of the right front middle of the culture device whole body, a cleaning device is arranged at the right end of the culture device whole body, the turnover cover is movably arranged at the top of the cleaning device, when a user uses the cleaning device to clean a culture dish, the cleaning device can utilize recycled cleaning water to clean the culture dish for the first time, then utilize unused cleaning water to clean the culture dish for the second time, and recycle the cleaning water for the second time, by the method, waste of water resources can be effectively reduced, and the cleaning effect of the culture dish can be effectively guaranteed.
Description
Technical Field
The invention belongs to the technical field of stem cell culture, and particularly relates to an adenocarcinoma stem cell culture device for breast cancer immunotherapy.
Background
Stem cells are a class of cells that have unlimited or immortal self-renewal capacity, capable of producing at least one type of highly differentiated progeny cells.
At present, current adenocarcinoma stem cell culture apparatus need take through manual aseptic lid to the culture dish top after putting into the culture dish, and can not cause the pollution because culture cell in the culture dish is not disinfected because of directly taking aseptic lid because culture apparatus's inner chamber, thereby lead to unable to cultivate adenocarcinoma stem cell, and current adenocarcinoma stem cell culture apparatus need wash the culture dish after using the completion, can be inconvenient fix the culture dish when wasing the culture dish and place, thereby make the user need spend more time to fix the culture dish, and can need use more clear water when wasing, and under the condition of global water resource antagonism, the action of water waste resource is not good.
Therefore, how to design an adenocarcinoma stem cell culture device for breast cancer immunotherapy becomes a problem to be solved at present.
Disclosure of Invention
The invention aims to: in order to solve the problems that the stem cells in the culture dish are easily polluted by the outside and the culture effect is low due to the fact that the cover of the culture dish is taken out in a manual taking mode when the culture device is opened, the culture dish is inconvenient to fix when being cleaned, and more water resources are wasted when the culture dish is cleaned, the adenocarcinoma stem cell culture device for breast cancer immunotherapy is provided.
The technical scheme adopted by the invention is as follows: an adenocarcinoma stem cell culture device for breast cancer immunotherapy comprises a culture device whole body, a cleaning device and a turnover cover, wherein a display screen is embedded in the top of the left side right in front of the culture device whole body, a control panel is embedded in the bottom of the left side right in front of the culture device whole body, a suction door is movably arranged in the middle of the top of the right front of the culture device whole body, the cleaning device is arranged at the right end of the culture device whole body, the turnover cover is movably arranged at the top of the cleaning device, a water inlet pipe is embedded in the top of the right side of the cleaning device, and a water drain pipe is embedded in the bottom of the right side of the cleaning device;
the left side of the top of the whole culture device is provided with a first driving motor, the middle of the top of the whole culture device is fixedly provided with a liquid pump, the bottom end of the liquid pump is communicated with a hydraulic telescopic rod, the bottom of the hydraulic telescopic rod is fixedly provided with a black cover cap, the left side of the inside of the cleaning device is movably provided with a cleaning brush head, the bottom end of the cleaning brush head is fixedly provided with a second driving motor, the right side of the inside of the cleaning device is provided with a water spray head, the bottom end of the water spray head is provided with a first three-way electromagnetic valve, the bottom of the middle of the first three-way electromagnetic valve is communicated with a conveying pipe, the bottom of the conveying pipe is communicated with a conveying pump, the periphery of the conveying pump is provided with a cleaning water recovery cavity, and the right side of the middle of the cleaning device is provided with a second three-way electromagnetic valve;
a third driving motor is arranged in the middle of the inside of the overturning cover, a limiting placing rotating plate is fixedly arranged at the bottom end of the third driving motor, an operating handle is embedded into the right side of the bottom of the limiting placing rotating plate, a transmission hinge is nested on the periphery of the top of the operating handle, a rotating threaded column is arranged on the inner left side of the transmission hinge in a linkage manner, a fixed column penetrates through the middle part of the rotating threaded column, a gear disc is fixedly arranged at the bottom end of the fixed column, a nested nut is nested on the periphery of the bottom of the rotating threaded column, and an elastic support column is fixedly arranged at the bottom of the nested nut;
the black cover is characterized in that a fourth driving motor is arranged in the middle of the inside of the black cover, a rolling disc is arranged in the front of the fourth driving motor in a linkage mode, pulling ropes are fixedly arranged on two sides of the rolling disc, a collision spring group is fixedly arranged at the bottom end of each pulling rope, and a protruding block is fixedly arranged on the outer side of each collision spring group.
Preferably, the periphery of the bottom of the first driving motor is provided with a ring gear in a linkage manner.
Preferably, the bottom of the left side of the ring gear is provided with an ultraviolet sterilization lamp.
Preferably, a steering pulley block is movably arranged on the inner side of the traction rope.
Preferably, an umbrella-shaped stop block is fixedly arranged at the middle part of the top of the second driving motor.
Preferably, the middle of the right side in the cleaning device is embedded with a filter element, and the periphery of the bottom of the filter element is fixedly provided with a filter element supporting seat.
Preferably, the inside of crossing the filter core supporting seat is provided with supporting spring group, the middle outside of crossing the filter core supporting seat is provided with the gum cover.
Preferably, the water inlet pipe is matched with the drain pipe, the water spraying head, the first three-way electromagnetic valve, the conveying pipe, the cleaning water recovery cavity, the conveying pump and the second three-way electromagnetic valve to form an energy-saving mechanism.
Preferably, the operating handle is matched with the transmission hinge, the rotary threaded column, the fixed column, the gear disc, the nested nut and the elastic support column for use, so that the cleaning and fixing mechanism is formed.
Preferably, the following components: the black cover cap is matched with the fourth driving motor, the rolling disc, the traction rope, the abutting spring group and the protruding block for use, so that the automatic cover taking mechanism is formed.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. according to the culture dish washing device, the energy-saving mechanism is arranged, so that when a user uses the washing device to wash the culture dish, the washing device can use recycled washing water to wash the culture dish for the first time, then uses unused washing water to wash the culture dish for the second time, and recycles the washing water for the second time, and by means of the method, waste of water resources can be effectively reduced, and the washing effect of the culture dish can be effectively guaranteed.
2. According to the culture dish cleaning device, the cleaning and fixing mechanism is arranged, so that when a user needs to clean a culture dish by using the cleaning device, the user can quickly and conveniently fix the culture dish by using the cleaning and fixing mechanism (the cleaning and fixing mechanism can effectively position the culture dish by using the gear disc arranged at the bottom end of the fixed column to enable the culture dish to be non-rotatable, then the culture dish can be effectively limited by using the elastic support columns which are turned outwards to enable the culture dish not to be separated from the fixed column), and the culture dish is fixedly placed by using the same structure arranged in the whole culture device.
3. According to the automatic cover taking mechanism, the top cover of the culture dish is not required to be opened by a user through opening the suction door, so that the culture dish is not polluted by external microorganisms and bacteria due to the fact that the suction door is opened, the top cover of the culture dish can be opened by the user more safely and conveniently, and the culture dish can be cultured under more excellent environmental conditions in the whole culture device.
Drawings
FIG. 1 is a schematic view of the overall structure of a culture apparatus according to the present invention;
FIG. 2 is a schematic view of the overall front cross-sectional structure of the culture apparatus of the present invention;
FIG. 3 is an enlarged schematic view of the structure at A of the present invention;
FIG. 4 is a front sectional view of the flip cover of the present invention;
FIG. 5 is an enlarged view of the structure at B of the present invention;
fig. 6 is a schematic front sectional view of the black cover according to the present invention.
The labels in the figure are: 1. the whole culture device; 2. a display screen; 3. a control panel; 4. closing the door; 5. a cleaning device; 6. turning over the cover; 601. a third drive motor; 602. the rotating plate is placed in a limiting manner; 603. an operating handle; 604. a drive hinge; 605. rotating the threaded column; 606. fixing a column; 607. a gear plate; 608. nesting nuts; 609. an elastomeric support column; 7. a water inlet pipe; 8. a drain pipe; 9. a first drive motor; 10. a ring gear; 11. an ultraviolet sterilizing lamp; 12. a liquid pump; 13. a hydraulic telescopic rod; 14. a black cover; 1401. a fourth drive motor; 1402. rolling up the disc; 1403. a hauling rope; 1404. a steering pulley block; 1405. against the spring set; 1406. a protruding block; 15. cleaning the brush head; 16. an umbrella-shaped stop block; 17. a second drive motor; 18. a sprinkler head; 19. a first three-way solenoid valve; 20. a delivery pipe; 21. a cleaning water recovery chamber; 22. a delivery pump; 23. a second three-way solenoid valve; 24. a filter element; 25. a filter element supporting seat; 26. a support spring set; 27. a rubber sleeve; 28. a culture dish.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance; furthermore, unless expressly stated or limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, as they may be fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Referring to fig. 1-6, the present invention provides a technical solution: an adenocarcinoma stem cell culture device for breast cancer immunotherapy comprises a culture device body 1, a cleaning device 5 and a turnover cover 6, wherein a display screen 2 is embedded in the top of the left side of the front of the culture device body 1, an operation panel 3 is embedded in the bottom of the left side of the front of the culture device body 1, a suction door 4 is movably arranged in the middle of the top of the front of the culture device body 1, the cleaning device 5 is arranged at the right end of the culture device body 1, the turnover cover 6 is movably arranged at the top of the cleaning device 5, a water inlet pipe 7 is embedded in the top of the right side of the cleaning device 5, and a water outlet pipe 8 is embedded in the bottom of the right side of the cleaning device 5;
a first driving motor 9 is arranged on the left side of the top of the whole culture device 1, a liquid pump 12 is fixedly arranged in the middle of the top of the whole culture device 1, a hydraulic telescopic rod 13 is communicated with the bottom end of the liquid pump 12, a black cover cap 14 is fixedly arranged at the bottom of the hydraulic telescopic rod 13, a cleaning brush head 15 is movably arranged on the left side of the inside of the cleaning device 5, a second driving motor 17 is fixedly arranged at the bottom end of the cleaning brush head 15, a water spray head 18 is arranged on the right side of the inside of the cleaning device 5, a first three-way electromagnetic valve 19 is arranged at the bottom end of the water spray head 18, a conveying pipe 20 is communicated with the bottom of the middle of the first three-way electromagnetic valve 19, a conveying pump 22 is communicated with the bottom of the conveying pipe 20, a cleaning water recovery cavity 21 is arranged on the periphery of the conveying pump 22, and a second three-way electromagnetic valve 23 is arranged on the right side of the middle of the cleaning device 5;
a third driving motor 601 is arranged in the middle of the inside of the turnover cover 6, a limiting placing rotating plate 602 is fixedly arranged at the bottom end of the third driving motor 601, an operating handle 603 is embedded into the right side of the bottom of the limiting placing rotating plate 602, a transmission hinge 604 is nested on the periphery of the top of the operating handle 603, a rotating threaded column 605 is arranged on the inner left side of the transmission hinge 604 in a linkage manner, a fixed column 606 penetrates through the middle part of the rotating threaded column 605, a gear disc 607 is fixedly arranged at the bottom end of the fixed column 606, a nested nut 608 is nested on the periphery of the bottom of the rotating threaded column 605, and an elastic supporting column 609 is fixedly arranged at the bottom of the nested nut 608;
a fourth driving motor 1401 is arranged in the middle of the inside of the black cover 14, a rolling disc 1402 is arranged right in front of the fourth driving motor 1401 in a linkage manner, pulling ropes 1403 are fixedly arranged on two sides of the rolling disc 1402, a supporting spring group 1405 is fixedly arranged at the bottom end of the pulling ropes 1403, and a protruding block 1406 is fixedly arranged on the outer side of the supporting spring group 1405.
Preferably, a ring gear 10 is arranged on the periphery of the bottom of the first driving motor 9 in a linkage manner, the ring gear 10 is circular in shape from the overlooking angle, a limiting sliding groove for rolling the ring gear 10 is arranged on the top inside the whole culture device 1, and insections matched with the bottom of the first driving motor 9 are arranged on the inner side of the ring gear 10, so that the ring gear 10 and the ultraviolet sterilization lamp 11 can be driven to rotate annularly inside the whole culture device 1 through the linkage gear disc on the periphery of the bottom when the first driving motor 9 operates.
Preferably, the left side bottom of ring gear 10 is provided with ultraviolet sterilamp 11, ultraviolet sterilamp 11 is rectangular shape, when ultraviolet sterilamp 11 rotated in the inside of the whole 1 of culture apparatus under the drive that receives ring gear 10, ultraviolet sterilamp 11 can effectually carry out germicidal treatment to the inside cavity of the whole 1 of culture apparatus, thereby make the whole 1 of culture apparatus can not have microorganism or bacterium to produce the pollution to the stem cell in the culture dish 28 in the culture chamber of the whole 1 inside setting of culture apparatus when opening the top cap of culture dish 28.
Preferably, the turning pulley block 1404 is movably disposed on the inner side of the pulling rope 1403, and four sets of turning pulley blocks 1404 are disposed on the turning pulley block 1404, so that when the pulling rope 1403 is driven by the rolling disc 1402 to contract, the pulling rope 1403 can be better wound around the periphery of the front of the rolling disc 1402 under the drive of the rolling disc 1402 through the turning pulley block 1404, and the pulling rope 1403 can be better moved inside the black cover 14 through the arrangement of the turning pulley block 1404.
Preferably, an umbrella-shaped stopper 16 is fixedly arranged at the middle part of the top of the second driving motor 17, and when the second driving motor 17 drives the cleaning brush head 15 to wipe and clean the culture dish 28, the umbrella-shaped stopper 16 can effectively prevent the cleaning water falling from the inner side of the culture dish 28 from falling into the outer side of the second driving motor 17, so that the cleaning water can not fall into the installation cavity of the second driving motor 17 through the connection gap between the second driving motor 17 and the inner side of the cleaning cavity inside the cleaning device 5 to affect the second driving motor 17 when falling freely.
Preferably, the filter element 24 is embedded in the middle of the right side inside the cleaning device 5, the filter element support seat 25 is fixedly arranged on the periphery of the bottom of the filter element 24, three filter layers, namely an activated carbon layer, a gravel layer and a filter cotton layer, are arranged inside the filter element 24, and the filter element 24 can effectively perform secondary filtration and recycling on cleaning water inside the cleaning device 5.
Preferably, the inside of crossing filter core supporting seat 25 is provided with supporting spring group 26, the middle outside of crossing filter core supporting seat 25 is provided with gum cover 27, when the user needs to change crossing filter core 24, the user accessible is manual to open the apron that sets up at belt cleaning device 5 right side middle part, then press toward the bottom through manual filter core 24 of holding of crossing for the supporting spring group 26 that sets up in crossing filter core supporting seat 25 inside compresses, then the user just can the manual filter core that will cross filter core 24 top orientation apron department that has opened of accessible will take out the filter core and change.
Preferably, the water inlet pipe 7 cooperates with the water outlet pipe 8, the water spraying head 18, the first three-way electromagnetic valve 19, the delivery pipe 20, the cleaning water recycling cavity 21, the delivery pump 22 and the second three-way electromagnetic valve 23 to form an energy-saving mechanism, when a user uses the cleaning device 5 to clean a used culture dish 28, the user needs to fix the culture dish 28 in the middle of the bottom of the limit placing rotating plate 602 by using the cleaning fixing mechanism, then the user can close the flip cover 6, after closing, the user can control the third driving motor 601 and the second driving motor 17 to operate simultaneously by using the control board 3 (the third driving motor 601 drives the limit placing rotating plate 602 to rotate clockwise, the second driving motor 17 drives the cleaning brush head 15 to rotate counterclockwise), and when the cleaning brush head 15 is used to clean the inner side of the culture dish 28, the conveying pump 22 conveys the filtered cleaning water in the cleaning water recovery cavity 21 to the water spraying head 18 through the conveying pipe 20 and the first three-way electromagnetic valve 19 to spray water to clean the inner side of the culture dish 28, the first three-way electromagnetic valve 19 is communicated with the conveying pipe 20 and is in a closed state with the water inlet pipe 7, the second three-way electromagnetic valve 23 is communicated with the water outlet pipe 8 and is in a closed state with the filter element 24, after the cleaning device 5 cleans the culture dish 28 for the first time, the first three-way electromagnetic valve 19 is communicated with the water inlet pipe 7, the second three-way electromagnetic valve 23 is communicated with the filter element 24, and the arrangement enables the cleaned water to flow into the filter element 24 through the second three-way electromagnetic valve 23 when the cleaning device 5 cleans the culture dish 28 for the second time by using clean water which is not used, and then flows back to the interior of the cleaning water recovery cavity 21 for standby after being filtered, through such setting make the user wash culture dish 28 using belt cleaning device 5, the usable recycle's of belt cleaning device 5 washing water washs culture dish 28 for the first time, and then the rethread utilizes unused washing water to wash culture dish 28 for the second time, and washs water to the second time and carries out recovery processing, and such practice can effectually reduce the waste of water resource, and can effectual assurance to the cleaning performance of culture dish 28.
Preferably, the operating handle 603 is used in cooperation with the transmission hinge 604, the rotating threaded column 605, the fixing column 606, the gear plate 607, the nesting nut 608 and the elastic support column 609 to form a cleaning and fixing mechanism, when a user needs to clean a used culture dish 28 by using the cleaning device 5, the user can manually open the flip cover 6, then manually align the slot formed at the bottom of the culture dish 28 with the fixing column 606 and the gear plate 607, after inserting, the user also needs to manually rotate the culture dish 28 to enable the gear groove formed at the bottom of the culture dish 28 and adapted to the gear plate 607 to be engaged with the gear plate 607, then the user can manually rotate the operating handle 603 clockwise, when the user manually rotates the operating handle 603 clockwise, the operating handle 603 drives the rotating threaded column 605 to rotate clockwise in place by driving the transmission hinge 604, when the rotating threaded column 605 rotates clockwise in situ, the nested nut 608 disposed in the middle of the rotating threaded column 605 cannot rotate clockwise with the rotating threaded column 605 because one end of the elastic support column 609 is fixed on the periphery of the fixed column 606, so that the nested nut 608 is pushed by the thread groove disposed on the periphery of the rotating threaded column 605 to move slowly to the top, and when the nested nut 608 moves to the top, the top of the elastic support column 609 serves as a supporting point to generate a pressing force on the elastic support column 609, so that the elastic support column 609 rotates outwards to deform, and when the elastic support column 609 rotates outwards to deform, the slot at the bottom of the culture dish 28 can be effectively limited, so that the culture dish 28 is firmly fixed at the current position and put into a cleaning stage, and by such an arrangement, when a user needs to clean the culture dish 28 by using the cleaning device 5, the user's accessible utilizes the quick convenient dish 28 of fixing mechanism that washs to fix (wash the effectual dish 28 of being fixed a position of gear 607 that fixing mechanism accessible fixed column 606 bottom set up, make it can not rotate, then the effectual dish 28 of being fixed of elastomeric support column 609 that the accessible turned outside makes it can not break away from fixed column 606), and the inside of the whole 1 of culture apparatus sets up the same structure and fixes placing dish 28 equally.
Preferably, the black cover 14 cooperates with the fourth driving motor 1401, the rolling disc 1402, the pulling rope 1403, the abutting spring group 1405 and the protruding block 1406 to form an automatic cover taking mechanism, after the user puts the petri dish 28 into the culture device whole 1 by opening the suction door 4, the user can control the liquid pump 12 to fill the liquid into the hydraulic telescopic rod 13 by using the control panel 3, so that the hydraulic telescopic rod 13 extends toward the bottom, and the hydraulic telescopic rod 13 can simultaneously push the round cover type black cover 14 to move toward the bottom when extending toward the bottom, so that the black cover 14 is attached to the bottom of the inner cavity of the culture device whole 1, and then the user can control the first driving motor 9 and the ultraviolet sterilizing lamp 11 to operate by using the control panel 3, so that the ultraviolet sterilizing lamp 11 can effectively sterilize the inside of the culture device whole 1, and because the whole black cover 14 is black and light-tight, therefore, the culture dish 28 placed inside is not affected, after the sterilization process is completed, the user can control the automatic cover taking mechanism to operate by using the control panel 3, and at this time, the fourth driving motor 1401 rotates counterclockwise, and when the fourth driving motor 1401 rotates counterclockwise, the rolling disc 1402 right in front of the fourth driving motor 1401 also rotates counterclockwise, and when the rolling disc 1402 rotates counterclockwise, the pulling rope 1403 wound around the periphery is deformed and extended to the bottom along the abutting spring group 1405 by the elastic force of the abutting spring group 1405, and when the abutting spring group 1405 is not pulled by the pulling rope 1403 and is restored to be deformed inward, the protruding block 1406 is pushed to move inward, and when the protruding block 1406 moves inward, the protruding block 1406 is attached to the periphery of the bottom of the culture dish 28, because the culture dish 28 is fixedly arranged inside the whole culture device 1 through the same mechanism as the cleaning and fixing mechanism, when the hydraulic telescopic rod 13 retracts, the protruding block 1406 can effectively take down the cover at the top of the culture dish 28, so that stem cells in the culture dish 28 can be better cultured in the environment provided in the whole culture device 1, and through the arrangement, a user does not need to open the top cover of the culture dish 28 by opening the suction door 4, so that the culture dish 28 cannot be polluted by the influence of external microorganisms and bacteria due to the opening of the suction door 4, the top cover of the culture dish 28 can be opened more safely and conveniently by the user, and further the culture dish 28 can be cultured under the more superior environmental conditions in the whole culture device 1.
The working principle is as follows: firstly, a user can put stem cells and prepared culture solution into the culture dish 28, then the culture dish 28 is fixedly placed inside the whole culture device 1 manually, after the culture dish is placed, the user can sterilize the internal gas by the ultraviolet sterilizing lamp arranged inside the whole culture device 1, then the top cover at the top of the culture dish 28 is taken by the automatic cover taking mechanism, so that the stem cells are better cultured inside the whole culture device 1, after the culture is finished, the user can fix the culture dish 28 in the middle of the inner side of the turnover cover 6 by utilizing the cleaning and fixing, and then the culture dish is cleaned by the cleaning device 5, and during the cleaning, the culture dish 28 can be cleaned for the first time by the recycled cleaning water by the energy-saving mechanism arranged in the cleaning device 5 and the cleaning water is discharged outwards, the culture dish 28 is washed for the second time by using unused washing water, and the washing water of the second washing is filtered and recycled, which is the working principle of the culture device for the adenocarcinoma stem cells for the breast cancer immunotherapy.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. An adenocarcinoma stem cell culture device for breast cancer immunotherapy, which comprises a culture device whole body (1), a cleaning device (5) and a turnover cover (6), and is characterized in that: a display screen (2) is embedded into the top of the left side right in front of the whole culture device (1), an operation control plate (3) is embedded into the bottom of the left side right in front of the whole culture device (1), a suction door (4) is movably arranged at the top of the middle of the right front of the whole culture device (1), a cleaning device (5) is arranged at the right end of the whole culture device (1), a turnover cover (6) is movably arranged at the top of the cleaning device (5), a water inlet pipe (7) is embedded into the top of the right side of the cleaning device (5), and a water outlet pipe (8) is embedded into the bottom of the right side of the cleaning device (5);
the device is characterized in that a first driving motor (9) is arranged on the left side of the top of the whole culture device (1), a liquid pump (12) is fixedly arranged in the middle of the top of the whole culture device (1), a hydraulic telescopic rod (13) is arranged in the bottom of the liquid pump (12) in a communicated manner, a black cover (14) is fixedly arranged at the bottom of the hydraulic telescopic rod (13), a cleaning brush head (15) is movably arranged on the left side of the inside of the cleaning device (5), a second driving motor (17) is fixedly arranged at the bottom of the cleaning brush head (15), a water spray head (18) is arranged on the right side of the inside of the cleaning device (5), a first three-way electromagnetic valve (19) is arranged at the bottom of the water spray head (18), a conveying pipe (20) is arranged in the bottom of the middle of the first three-way electromagnetic valve (19) in a communicated manner, and a conveying pump (22) is arranged in the bottom of the conveying pipe (20), a cleaning water recovery cavity (21) is arranged on the periphery of the conveying pump (22), and a second three-way electromagnetic valve (23) is arranged on the right side of the middle of the cleaning device (5);
a third driving motor (601) is arranged in the middle of the inside of the turnover cover (6), a limiting placing rotating plate (602) is fixedly arranged at the bottom end of the third driving motor (601), an operating handle (603) is embedded into the right side of the bottom of the limiting placing rotating plate (602), a transmission hinge (604) is nested on the periphery of the top of the operating handle (603), a rotating threaded column (605) is arranged on the inner left side of the transmission hinge (604) in a linkage manner, a fixed column (606) is arranged in the middle of the rotating threaded column (605) in a penetrating manner, a gear disc (607) is fixedly arranged at the bottom end of the fixed column (606), a nested nut (608) is nested on the periphery of the bottom of the rotating threaded column (605), and an elastic supporting column (609) is fixedly arranged at the bottom of the nested nut (608);
the black cover is characterized in that a fourth driving motor (1401) is arranged in the middle of the inside of the black cover (14), a rolling disc (1402) is arranged in the front of the fourth driving motor (1401) in a linkage mode, pulling ropes (1403) are fixedly arranged on two sides of the rolling disc (1402), a collision spring group (1405) is fixedly arranged at the bottom end of each pulling rope (1403), and a protruding block (1406) is fixedly arranged on the outer side of each collision spring group (1405).
2. The device of claim 1, wherein the culture device comprises: and a ring gear (10) is arranged at the periphery of the bottom of the first driving motor (9) in a linkage manner.
3. The device of claim 2, wherein the culture device comprises: and an ultraviolet sterilizing lamp (11) is arranged at the bottom of the left side of the ring gear (10).
4. The device of claim 1, wherein the culture device comprises: and a steering pulley block (1404) is movably arranged on the inner side of the traction rope (1403).
5. The device of claim 1, wherein the culture device comprises: an umbrella-shaped stop block (16) is fixedly arranged at the middle part of the top of the second driving motor (17).
6. The device of claim 1, wherein the culture device comprises: the middle of the right side in the cleaning device (5) is embedded with a filter core (24), and the periphery of the bottom of the filter core (24) is fixedly provided with a filter core supporting seat (25).
7. The device of claim 6, wherein the culture device comprises: the filter core is characterized in that a supporting spring set (26) is arranged inside the filter core supporting seat (25), and a rubber sleeve (27) is arranged on the outer side of the middle of the filter core supporting seat (25).
8. The device of claim 1, wherein the culture device comprises: the water inlet pipe (7) is matched with a water outlet pipe (8), a water spraying head (18), a first three-way electromagnetic valve (19), a conveying pipe (20), a cleaning water recovery cavity (21), a conveying pump (22) and a second three-way electromagnetic valve (23) to form an energy-saving mechanism.
9. The device of claim 1, wherein the culture device comprises: the operation handle (603) is matched with the transmission hinge (604), the rotary threaded column (605), the fixing column (606), the gear disc (607), the nested nut (608) and the elastic support column (609) to form a cleaning fixing mechanism.
10. The device of claim 1, wherein the culture device comprises: the black cover (14) is matched with a fourth driving motor (1401), a rolling disc (1402), a traction rope (1403), a propping spring group (1405) and a protruding block (1406) for use together to form an automatic cover taking mechanism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210038165.2A CN114381371B (en) | 2022-01-13 | 2022-01-13 | Adenocarcinoma stem cell culture device for breast cancer immunotherapy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210038165.2A CN114381371B (en) | 2022-01-13 | 2022-01-13 | Adenocarcinoma stem cell culture device for breast cancer immunotherapy |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114381371A true CN114381371A (en) | 2022-04-22 |
| CN114381371B CN114381371B (en) | 2023-11-07 |
Family
ID=81201372
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210038165.2A Active CN114381371B (en) | 2022-01-13 | 2022-01-13 | Adenocarcinoma stem cell culture device for breast cancer immunotherapy |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114381371B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117683634A (en) * | 2024-01-31 | 2024-03-12 | 山东格林医学科技有限公司 | Stem cell culture device and method for diabetes treatment |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011087053A1 (en) * | 2010-01-13 | 2011-07-21 | 株式会社日立製作所 | Automated culture device, and culture vessel installation method |
| WO2017202192A1 (en) * | 2016-05-27 | 2017-11-30 | 中国科学院广州生物医药与健康研究院 | Multi-purpose tool tip for stem cell induction and culturing apparatus, and stem cell induction and culturing apparatus |
| CN108715806A (en) * | 2018-06-07 | 2018-10-30 | 陈龙刚 | Automatic cleaning equipment for cultivating tissue block needed for stem cell |
| CN111112265A (en) * | 2018-11-01 | 2020-05-08 | 江苏优仕生物科技发展有限公司 | Microbial cultivation ware cleaning and sterilizing device |
| CN211217920U (en) * | 2019-12-03 | 2020-08-11 | 瑞太干细胞中心(沈阳)有限公司 | Stem cell household utensils disinfection belt cleaning device |
| CN211535761U (en) * | 2019-12-28 | 2020-09-22 | 沈阳达安医学检验所有限公司 | Culture dish sterilizing equipment for genetic engineering |
| CN113020177A (en) * | 2021-03-05 | 2021-06-25 | 陈俊锦 | High-efficient belt cleaning device of test tube that uses during stem cell culture |
| CN215250927U (en) * | 2021-05-31 | 2021-12-21 | 杭州三江上御生物科技有限公司 | Multifunctional adipose-derived stem cell culture dish |
-
2022
- 2022-01-13 CN CN202210038165.2A patent/CN114381371B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011087053A1 (en) * | 2010-01-13 | 2011-07-21 | 株式会社日立製作所 | Automated culture device, and culture vessel installation method |
| WO2017202192A1 (en) * | 2016-05-27 | 2017-11-30 | 中国科学院广州生物医药与健康研究院 | Multi-purpose tool tip for stem cell induction and culturing apparatus, and stem cell induction and culturing apparatus |
| CN108715806A (en) * | 2018-06-07 | 2018-10-30 | 陈龙刚 | Automatic cleaning equipment for cultivating tissue block needed for stem cell |
| CN111112265A (en) * | 2018-11-01 | 2020-05-08 | 江苏优仕生物科技发展有限公司 | Microbial cultivation ware cleaning and sterilizing device |
| CN211217920U (en) * | 2019-12-03 | 2020-08-11 | 瑞太干细胞中心(沈阳)有限公司 | Stem cell household utensils disinfection belt cleaning device |
| CN211535761U (en) * | 2019-12-28 | 2020-09-22 | 沈阳达安医学检验所有限公司 | Culture dish sterilizing equipment for genetic engineering |
| CN113020177A (en) * | 2021-03-05 | 2021-06-25 | 陈俊锦 | High-efficient belt cleaning device of test tube that uses during stem cell culture |
| CN215250927U (en) * | 2021-05-31 | 2021-12-21 | 杭州三江上御生物科技有限公司 | Multifunctional adipose-derived stem cell culture dish |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117683634A (en) * | 2024-01-31 | 2024-03-12 | 山东格林医学科技有限公司 | Stem cell culture device and method for diabetes treatment |
| CN117683634B (en) * | 2024-01-31 | 2024-05-07 | 山东格林医学科技有限公司 | Stem cell culture device and method for diabetes treatment |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114381371B (en) | 2023-11-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN205987943U (en) | A kind of water circle device for fruit cleaning | |
| CN114381371A (en) | A adenocarcinoma stem cell culture apparatus for breast cancer immunotherapy | |
| CN108585302B (en) | Domestic sewage treatment equipment for environmental protection | |
| CN213265891U (en) | Biological aerated filter with good backwashing effect | |
| CN211679200U (en) | Test tube cleaning device for bio-pharmaceuticals | |
| CN210187902U (en) | MBR is belt cleaning device for sewage treatment system | |
| CN210302751U (en) | Domestic sewage treatment equipment | |
| CN213084325U (en) | Classified compression and storage device for municipal refuse | |
| CN210304738U (en) | Novel biological experimental device cleaning device | |
| CN209404490U (en) | A kind of automatic flushing device of water purifier | |
| CN209020020U (en) | A kind of drum filter and its water process circulatory system | |
| CN206701878U (en) | Multifunctional chemical bottle cleaning equipment | |
| CN213204361U (en) | Water-saving toilet | |
| CN212576978U (en) | Medical clean sealing device | |
| CN221108284U (en) | Chemistry experiment waste liquid collection device | |
| CN221238693U (en) | Sewage treatment sampling device | |
| CN211170650U (en) | Domestic fungus fermentation cylinder | |
| CN213595114U (en) | From environmental protection garbage bin of taking sewage treatment function | |
| CN221014883U (en) | Multistage filtering device for biofertilizer | |
| CN212069716U (en) | A clean bench for animal experiment | |
| CN213169670U (en) | Medical treatment waste liquid collection device | |
| CN211158795U (en) | Front-mounted backwashing filter | |
| CN214192953U (en) | Biological membrane sewage treatment device | |
| CN214360571U (en) | Active carbon filter equipment is used in system water workshop | |
| CN204171058U (en) | Beaker cleaning apparatus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |