CN114381371B - Adenocarcinoma stem cell culture device for breast cancer immunotherapy - Google Patents
Adenocarcinoma stem cell culture device for breast cancer immunotherapy Download PDFInfo
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- CN114381371B CN114381371B CN202210038165.2A CN202210038165A CN114381371B CN 114381371 B CN114381371 B CN 114381371B CN 202210038165 A CN202210038165 A CN 202210038165A CN 114381371 B CN114381371 B CN 114381371B
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 27
- 238000004113 cell culture Methods 0.000 title claims abstract description 19
- 208000009956 adenocarcinoma Diseases 0.000 title claims abstract description 18
- 206010006187 Breast cancer Diseases 0.000 title claims abstract description 16
- 208000026310 Breast neoplasm Diseases 0.000 title claims abstract description 16
- 238000002619 cancer immunotherapy Methods 0.000 title claims abstract description 9
- 238000004140 cleaning Methods 0.000 claims abstract description 96
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 57
- 230000007246 mechanism Effects 0.000 claims abstract description 21
- 230000007306 turnover Effects 0.000 claims abstract description 10
- 230000001954 sterilising effect Effects 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 11
- 238000005096 rolling process Methods 0.000 claims description 10
- 238000011084 recovery Methods 0.000 claims description 9
- 239000007921 spray Substances 0.000 claims description 9
- 230000005540 biological transmission Effects 0.000 claims description 8
- 238000005507 spraying Methods 0.000 claims description 3
- 238000004804 winding Methods 0.000 claims description 3
- 238000009169 immunotherapy Methods 0.000 claims 6
- 230000000149 penetrating effect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 description 7
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 244000181917 Rubus leucodermis Species 0.000 description 1
- 235000011036 Rubus leucodermis Nutrition 0.000 description 1
- 235000003942 Rubus occidentalis Nutrition 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/20—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought
- B08B9/28—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought the apparatus cleaning by splash, spray, or jet application, with or without soaking
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/20—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought
- B08B9/36—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought the apparatus cleaning by using brushes
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- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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Abstract
The invention belongs to the technical field of stem cell culture, and particularly discloses an adenocarcinoma stem cell culture device for breast cancer immunotherapy, which comprises a culture device whole, a cleaning device and a turnover cover, wherein a display screen is embedded in the top of the left side of the front of the culture device whole, a control panel is embedded in the bottom of the left side of the front of the culture device whole, a sucking door is movably arranged at the middle top of the front of the culture device whole, the right end of the culture device whole is provided with the cleaning device, the top of the cleaning device is movably provided with the turnover cover, and an energy-saving mechanism can utilize recycled cleaning water to clean the culture dish for the first time when a user uses the cleaning device to clean the culture dish, and then utilize unused cleaning water to clean the culture dish for the second time, and recycle the cleaning water for the second time.
Description
Technical Field
The invention belongs to the technical field of stem cell culture, and particularly relates to an adenocarcinoma stem cell culture device for breast cancer immunotherapy.
Background
Stem cells are a class of cells with unlimited or immortal self-renewing capacity capable of producing at least one type of highly differentiated daughter cells.
At present, current adenocarcinoma stem cell culture apparatus need take the aseptic lid of culture dish top through manual after putting into the culture dish, and because directly take aseptic lid can not carry out the sterilization because the inner chamber of culture apparatus and cause the pollution to the culture cell in the culture dish, thereby lead to unable to cultivate adenocarcinoma stem cell, and current adenocarcinoma stem cell culture apparatus need wash the culture dish after accomplishing, can be inconvenient when wasing the culture dish fixed placement to the culture dish, thereby make the user need spend more time to fix the culture dish, and can need to use more clear water when wasing, and under the condition that global water resource is according to the resource, the action of wasting water resource is not appropriate.
Therefore, how to design an adenocarcinoma stem cell culture device for breast cancer immunotherapy is a problem to be solved at present.
Disclosure of Invention
The invention aims at: in order to solve the problems that the culture effect is low, the culture dish is inconvenient to fix when the culture dish is cleaned, and more water resources are wasted when the culture dish is cleaned due to the fact that the cover of the culture dish is taken out manually when the culture apparatus is opened, so that stem cells in the culture dish are easy to be polluted by the outside, the adenocarcinoma stem cell culture apparatus for breast cancer immunotherapy is provided.
The technical scheme adopted by the invention is as follows: the utility model provides an adenocarcinoma stem cell culture apparatus for breast cancer immunotherapy, includes culture apparatus whole, belt cleaning device and flip cover, the embedding of culture apparatus whole dead ahead left side top is provided with the display screen, the embedding of culture apparatus whole dead ahead left side bottom is provided with the control board, the activity of culture apparatus whole dead ahead middle top is provided with the actuation door, the holistic right-hand member of culture apparatus is provided with belt cleaning device, belt cleaning device's top activity is provided with flip cover, belt cleaning device's right side top embedding is provided with the inlet tube, belt cleaning device's right side bottom embedding is provided with the drain pipe;
the device comprises a culture device, and is characterized in that a first driving motor is arranged on the left side of the top of the whole culture device, a liquid pump is fixedly arranged in the middle of the top of the whole culture device, a hydraulic telescopic rod is arranged at the bottom end of the liquid pump in a communicating manner, a black cover is fixedly arranged at the bottom of the hydraulic telescopic rod, a cleaning brush head is movably arranged on the left side of the interior of the cleaning device, a second driving motor is fixedly arranged at the bottom end of the cleaning brush head, a water spray head is arranged on the right side of the interior of the cleaning device, a first three-way electromagnetic valve is arranged at the bottom end of the water spray head, a conveying pipe is arranged at the middle bottom of the first three-way electromagnetic valve in a communicating manner, a conveying pump is arranged at the bottom of the conveying pipe in a communicating manner, a cleaning water recovery cavity is arranged at the periphery of the conveying pump, and a second three-way electromagnetic valve is arranged on the right side of the middle of the cleaning device;
a third driving motor is arranged in the middle of the inside of the turnover cover, a limiting placing rotating plate is fixedly arranged at the bottom end of the third driving motor, an operation handle is embedded in the right side of the bottom of the limiting placing rotating plate, a transmission hinge is arranged on the periphery of the top of the operation handle in a nested mode, a rotary threaded column is arranged on the inner left side of the transmission hinge in a linkage mode, a fixing column penetrates through the middle portion of the rotary threaded column, a gear disc is fixedly arranged at the bottom end of the fixing column, a nested nut is arranged on the periphery of the bottom of the rotary threaded column in a nested mode, and an elastic support column is fixedly arranged at the bottom of the nested nut;
the black cover is characterized in that a fourth driving motor is arranged in the middle of the inside of the black cover, a rolling disc is arranged in front of the fourth driving motor in a linkage mode, traction ropes are fixedly arranged on two sides of the rolling disc, an abutting spring group is arranged at the bottom end of each traction rope, and a protruding block is fixedly arranged on the outer side of each abutting spring group.
Preferably, a ring gear is arranged at the periphery of the bottom of the first driving motor in a linkage manner.
Preferably, an ultraviolet sterilizing lamp is arranged at the bottom of the left side of the ring gear.
Preferably, a steering pulley block is movably arranged on the inner side of the traction rope.
Preferably, an umbrella-shaped stop block is fixedly arranged at the middle part of the top of the second driving motor.
Preferably, the middle of the right side of the cleaning device is embedded with a filter element, and a filter element supporting seat is fixedly arranged on the periphery of the bottom of the filter element.
Preferably, a supporting spring group is arranged in the filter core supporting seat, and a rubber sleeve is arranged on the outer side of the middle of the filter core supporting seat.
Preferably, the water inlet pipe is matched with the water outlet pipe, the water spraying head, the first three-way electromagnetic valve, the conveying pipe, the cleaning water recycling cavity, the conveying pump and the second three-way electromagnetic valve together to form an energy-saving mechanism.
Preferably, the operating handle is matched with the transmission hinge, the rotary threaded column, the fixed column, the gear disc, the nested nut and the elastic support column together to form the cleaning and fixing mechanism.
Preferably, the method comprises the steps of: the black cover cap is matched with the fourth driving motor, the winding disc, the traction rope, the abutting spring set and the protruding block together to form an automatic cover taking mechanism.
In summary, due to the adoption of the technical scheme, the beneficial effects of the invention are as follows:
1. according to the invention, the energy-saving mechanism is arranged, so that when a user uses the cleaning device to clean the culture dish, the cleaning device can utilize recycled cleaning water to clean the culture dish for the first time, then the unused cleaning water is used to clean the culture dish for the second time, and the cleaning water for the second time is recycled, thereby effectively reducing the waste of water resources and effectively ensuring the cleaning effect of the culture dish.
2. According to the invention, the cleaning and fixing mechanism is arranged, so that a user can quickly and conveniently fix the culture dish by using the cleaning and fixing mechanism when the user needs to clean the culture dish by using the cleaning and fixing mechanism (the cleaning and fixing mechanism can effectively fix the culture dish through the gear disc arranged at the bottom end of the fixing column so as to prevent the rotation of the culture dish, and then the culture dish can be effectively limited through the elastic support column which turns outwards so as not to be separated from the fixing column), and the culture dish is fixedly placed by using the same structure in the whole culture device.
3. According to the automatic cover taking mechanism, a user does not need to open the top cover of the culture dish by opening the suction door through the arrangement, so that the culture dish cannot be polluted by external microorganisms and bacteria due to the fact that the suction door is opened, the user can open the top cover of the culture dish safely and conveniently, and the culture dish can be cultivated under more superior environmental conditions in the whole culture device.
Drawings
FIG. 1 is a schematic diagram showing the overall structure of a culture apparatus according to the present invention;
FIG. 2 is a schematic diagram showing the whole cross-sectional elevation structure of the culture apparatus of the present invention;
FIG. 3 is an enlarged schematic view of the structure of the present invention at A;
FIG. 4 is a schematic diagram showing the front cross-sectional structure of the flip cover of the present invention;
FIG. 5 is an enlarged schematic view of the structure of the present invention at B;
fig. 6 is a schematic diagram showing a front sectional structure of the black cap of the present invention.
The marks in the figure: 1. the whole culture device; 2. a display screen; 3. a control panel; 4. a door; 5. a cleaning device; 6. a flip cover; 601. a third driving motor; 602. limiting and placing a rotating plate; 603. an operating handle; 604. a drive hinge; 605. rotating the threaded column; 606. fixing the column; 607. a gear plate; 608. a nested nut; 609. an elastomeric support column; 7. a water inlet pipe; 8. a drain pipe; 9. a first driving motor; 10. a ring gear; 11. an ultraviolet sterilizing lamp; 12. a liquid pump; 13. a hydraulic telescopic rod; 14. a black cover; 1401. a fourth driving motor; 1402. a winding disc; 1403. a traction rope; 1404. a steering pulley block; 1405. a set of abutting springs; 1406. a protruding block; 15. a cleaning brush head; 16. umbrella-shaped stop block; 17. a second driving motor; 18. a water spray head; 19. a first three-way electromagnetic valve; 20. a delivery tube; 21. a washing water recovery chamber; 22. a transfer pump; 23. a second three-way electromagnetic valve; 24. a filter element; 25. a filter core support seat; 26. a support spring group; 27. a rubber sleeve; 28. a culture dish.
Detailed Description
The present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
In the description of the present invention, it should be noted that, directions or positional relationships indicated by terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., are based on directions or positional relationships shown in the drawings, are merely for convenience of description and simplification of description, and do not indicate or imply that the apparatus or element to be referred to must have a specific direction, be constructed and operated in the specific direction, and thus should not be construed as limiting the present invention; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance; furthermore, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
Referring to fig. 1-6, the present invention provides a technical solution: the utility model provides an adenocarcinoma stem cell culture device for breast cancer immunotherapy, including culture device whole 1, belt cleaning device 5 and flip cover 6, the embedding of the right front left side top of culture device whole 1 is provided with display screen 2, the embedding of the right front left side bottom of culture device whole 1 is provided with controls board 3, the activity of the middle top in the right front of culture device whole 1 is provided with actuation door 4, the right-hand member of culture device whole 1 is provided with belt cleaning device 5, the activity of the top of belt cleaning device 5 is provided with flip cover 6, the embedding of the right side top of belt cleaning device 5 is provided with inlet tube 7, the embedding of the right side bottom of belt cleaning device 5 is provided with drain pipe 8;
the left side of the top of the whole culture device 1 is provided with a first driving motor 9, the middle of the top of the whole culture device 1 is fixedly provided with a liquid pump 12, the bottom end of the liquid pump 12 is communicated with a hydraulic telescopic rod 13, the bottom of the hydraulic telescopic rod 13 is fixedly provided with a black cover 14, the left side of the interior of the cleaning device 5 is movably provided with a cleaning brush head 15, the bottom end of the cleaning brush head 15 is fixedly provided with a second driving motor 17, the right side of the interior of the cleaning device 5 is provided with a water spray head 18, the bottom end of the water spray head 18 is provided with a first three-way electromagnetic valve 19, the middle bottom of the first three-way electromagnetic valve 19 is communicated with a conveying pipe 20, the bottom of the conveying pipe 20 is communicated with a conveying pump 22, the periphery of the conveying pump 22 is provided with a cleaning water recovery cavity 21, and the right side of the middle of the cleaning device 5 is provided with a second three-way electromagnetic valve 23;
a third driving motor 601 is arranged in the middle of the inside of the turnover cover 6, a limiting placing rotary plate 602 is fixedly arranged at the bottom end of the third driving motor 601, an operation handle 603 is embedded in the right side of the bottom of the limiting placing rotary plate 602, a transmission hinge 604 is arranged on the periphery of the top of the operation handle 603 in a nested manner, a rotary threaded column 605 is arranged on the inner left side of the transmission hinge 604 in a linkage manner, a fixing column 606 penetrates through the middle part of the rotary threaded column 605, a gear disc 607 is fixedly arranged at the bottom end of the fixing column 606, a nested nut 608 is arranged on the periphery of the bottom of the rotary threaded column 605 in a nested manner, and an elastic support column 609 is fixedly arranged at the bottom of the nested nut 608;
a fourth driving motor 1401 is arranged in the middle of the inside of the black cover 14, a rolling disc 1402 is arranged in linkage right in front of the fourth driving motor 1401, traction ropes 1403 are fixedly arranged on two sides of the rolling disc 1402, a supporting spring group 1405 is fixedly arranged at the bottom end of each traction rope 1403, and a protruding block 1406 is fixedly arranged on the outer side of each supporting spring group 1405.
Preferably, the bottom periphery of the first driving motor 9 is provided with a ring gear 10 in a linkage manner, the ring gear 10 is in a circular ring shape in a overlook view, a limiting chute for rolling the ring gear 10 is arranged at the top of the inside of the whole culture device 1, and the inner side of the ring gear 10 is provided with a tooth pattern matched with the bottom of the first driving motor 9, so that the ring gear 10 and the ultraviolet sterilization lamp 11 can be driven to rotate in a circular manner in the inside of the whole culture device 1 through a linkage gear disc at the periphery of the bottom when the first driving motor 9 operates.
Preferably, the left side bottom of ring gear 10 is provided with ultraviolet sterilizing lamp 11, and ultraviolet sterilizing lamp 11 is rectangular shape, and when ultraviolet sterilizing lamp 11 received ring gear 10 to drive down in the inside of culture apparatus whole 1 to rotate, ultraviolet sterilizing lamp 11 can effectually disinfect to the inside cavity of culture apparatus whole 1 to make culture apparatus whole 1 can not have microorganism or bacterium to produce the pollution to the stem cell in the culture dish 28 in the culture cavity that culture apparatus whole 1 set up when opening the top cap of culture dish 28.
Preferably, the inside activity of hauling rope 1403 is provided with steering pulley block 1404, and steering pulley block 1404 is provided with four groups, and it can be effectual when making the hauling rope 1403 shrink under the drive of reel 1402, can be better twine in the periphery in the dead ahead of reel 1402 through steering pulley block 1404 under the drive of reel 1402, makes the hauling rope 1403 can be better remove in the inside of black shroud 14 through setting up of steering pulley 1404.
Preferably, an umbrella-shaped stop 16 is fixedly arranged at the middle part of the top of the second driving motor 17, when the second driving motor 17 drives the cleaning brush head 15 to wipe and clean the culture dish 28, the umbrella-shaped stop 16 can effectively prevent cleaning water falling from the inner side of the culture dish 28 from falling into the outer side of the second driving motor 17, so that the cleaning water can not fall into the mounting cavity of the second driving motor 17 to influence the second driving motor 17 through a connecting gap between the second driving motor 17 and the inner side of the cleaning cavity in the cleaning device 5 when falling freely.
Preferably, the filter core 24 is embedded in the middle of the right side of the inside of the cleaning device 5, the filter core supporting seat 25 is fixedly arranged on the periphery of the bottom of the filter core 24, three layers of filter layers are respectively arranged in the filter core 24, namely an active carbon layer, a gravel layer and a filter cotton layer, and the filter core 24 can effectively carry out secondary filtration and recycling on cleaning water in the cleaning device 5.
Preferably, the inside of filter core supporting seat 25 is provided with support spring group 26, the middle outside of filter core supporting seat 25 is provided with gum cover 27, when the user needs to change filter core 24, the apron of setting at belt cleaning device 5 right side middle part is opened to the user's accessible manual, then presses to the bottom through manual holding filter core 24 for the support spring group 26 that sets up in filter core supporting seat 25 inside compresses, then the user just can take out the filter core with filter core 24 top orientation cover department that has opened through manual and change.
Preferably, the water inlet pipe 7 is matched with the water outlet pipe 8, the water spraying head 18, the first three-way electromagnetic valve 19, the conveying pipe 20, the cleaning water recovery cavity 21, the conveying pump 22 and the second three-way electromagnetic valve 23 to form an energy-saving mechanism, when a user uses the cleaning device 5 to clean the used culture dish 28, the user needs to fix the culture dish 28 in the middle of the bottom of the limiting rotating plate 602 by using the cleaning fixing mechanism, then the user can close the turnover cover 6, after the turnover cover is closed, the user can control the third driving motor 601 and the second driving motor 17 to operate simultaneously by using the control plate 3 (the third driving motor 601 drives the limiting rotating plate 602 to rotate clockwise when operating and the second driving motor 17 drives the cleaning brush head 15 to rotate anticlockwise when the cleaning brush head 15 rotates the inner side of the culture dish 28, the delivery pump 22 delivers the filtered cleaning water in the cleaning water recovery cavity 21 to the water spray head 18 through the delivery pipe 20 and the first three-way electromagnetic valve 19 to spray water to clean the inner side of the culture dish 28, the first three-way electromagnetic valve 19 is communicated with the delivery pipe 20 and is in a closed state with the water inlet pipe 7, the second three-way electromagnetic valve 23 is communicated with the water outlet pipe 8 and is in a closed state with the filter core 24, the first three-way electromagnetic valve 19 is communicated with the water inlet pipe 7 and the second three-way electromagnetic valve 23 is communicated with the filter core 24 after the culture dish 28 is cleaned by the cleaning device 5 for the first time, the arrangement is such that when the culture dish 28 is cleaned twice by clean water which is not used by the cleaning device 5, the cleaned water flows into the filter core 24 through the second three-way electromagnetic valve 23, then back flows to the inside of cleaning water recovery chamber 21 through filtering back and reserve, make the user when using belt cleaning device 5 to wash culture dish 28 through such setting, the usable recycle's of belt cleaning device 5 cleaning water carries out the first time to culture dish 28 and washs, then the rethread utilizes unused cleaning water to carry out the secondary to culture dish 28 and carries out recovery processing to the abluent cleaning water of secondary, and the waste of water resource can effectually be reduced to this kind of way, and can effectually guarantee the cleaning performance to culture dish 28.
Preferably, the operating handle 603 is used together with the driving hinge 604, the rotating threaded column 605, the fixing column 606, the gear disk 607, the nested nut 608 and the elastic support column 609 to form a cleaning fixing mechanism, when a user needs to clean the used culture dish 28 by using the cleaning device 5, the user can manually open the turnover cover 6, then manually align the slot arranged at the bottom of the culture dish 28 with the fixing column 606 and the gear disk 607, after the insertion, the user also needs to manually rotate the culture dish 28, so that the gear slot arranged at the bottom of the culture dish 28 and matched with the gear disk 607 is in meshed connection with the gear disk 607, after that, the user can manually rotate the operating handle 603 clockwise, when the user rotates the operating handle 603 clockwise by hand, the operating handle 603 can drive the rotating threaded column 605 to rotate clockwise in place by driving the driving hinge 604, when the rotary threaded column 605 rotates clockwise in situ, the nested nut 608 arranged in the middle of the rotary threaded column 605 can not rotate clockwise along with the rotary threaded column 605 because one end of the elastic support column 609 is fixed on the periphery of the fixed column 606, so the nested nut 608 can slowly move to the top under the pushing of the thread groove arranged on the periphery of the rotary threaded column 605, when the nested nut 608 moves to the top, the top of the elastic support column 609 is taken as a supporting point to generate extrusion force for the elastic support column 609, so that the elastic support column 609 turns outwards to deform, and when the elastic support column 609 turns outwards to deform, the limit treatment can be effectively carried out on the slot at the bottom of the culture dish 28, so that the culture dish 28 is firmly fixed at the current position and put into the cleaning stage, through such setting makes the user when needs wash culture dish 28 through utilizing belt cleaning device 5, and the user's accessible utilizes washs quick convenient to culture dish 28 of fixed establishment (washs the effectual culture dish 28 of gear wheel 607 that fixed establishment accessible fixed column 606 bottom set up and fix a position, makes it irrotational, and afterwards the accessible is outside the elastic support column 609 of upset effectually carries out spacingly to culture dish 28, makes it can not break away from fixed column 606), and culture apparatus whole 1's inside sets up the same structure equally and fixes the placing to culture dish 28.
Preferably, the black cover 14 is used together with the fourth driving motor 1401, the rolling disc 1402, the traction rope 1403, the abutting spring set 1405 and the protruding block 1406 to form an automatic cover taking mechanism, after a user puts the culture dish 28 into the whole culture device 1 by opening the suction door 4, the user can fill the liquid pump 12 with the liquid pump 13 by using the control plate 3 to enable the liquid pump 13 to extend towards the bottom, and when the liquid pump 13 extends towards the bottom, the black cover 14 with the round cover is pushed to move towards the bottom at the same time, so that the black cover 14 is attached to the bottom of the inner cavity of the whole culture device 1, then the user can control the first driving motor 9 and the ultraviolet sterilization lamp 11 to operate by using the control plate 3, so that the ultraviolet sterilization lamp 11 can effectively sterilize the inside of the whole culture device 1, the black cover 14 is black and opaque, so that the internal culture dish 28 is not affected, after the sterilization process is completed, the user can control the automatic cover taking mechanism to operate by using the control board 3, and the fourth driving motor 1401 rotates anticlockwise, and when the fourth driving motor 1401 rotates anticlockwise, the rolling disc 1402 in front of the fourth driving motor 1401 rotates anticlockwise, and when the rolling disc 1402 rotates anticlockwise, the traction rope 1403 wound around the periphery is influenced by the elastic force of the abutting spring group 1405, and extends to the bottom through the deformation of the abutting spring group 1405 by the elastic force of the abutting spring group 1404, and when the abutting spring group 1405 is not pulled by the traction rope 1403 and returns to the inner deformation, the protruding block 1406 is pushed to move inwards simultaneously, the protruding block 1406 will be attached to the periphery of the bottom of the culture dish 28 when moving inwards, and because the culture dish 28 is fixedly arranged inside the culture apparatus body 1 through the mechanism which is the same as that of cleaning and fixing, when the hydraulic telescopic rod 13 is retracted, the protruding block 1406 can effectively take down the cover at the top of the culture dish 28, so that stem cells in the culture dish 28 can be better cultured in the environment provided in the culture apparatus body 1, and by the arrangement, a user does not need to open the top cover of the culture dish 28 by opening the suction door 4, so that the culture dish 28 cannot be polluted by external microorganisms and bacteria due to the influence of opening the suction door 4, and the user can open the top cover of the culture dish 28 more safely and conveniently, so that the culture dish 28 can be cultured under more superior environmental conditions in the culture apparatus body 1.
Working principle: firstly, the user can put stem cells and prepared culture solution into the culture dish 28, then manually fix the culture dish 28 in the culture apparatus 1, after the culture dish is put, the user can sterilize the internal gas through an ultraviolet sterilizing lamp arranged in the culture apparatus 1, then take a top cover at the top of the culture dish 28 through an automatic cover taking mechanism, so that the stem cells can be better cultured in the culture apparatus 1, after the culture is finished, the user can fix the culture dish 28 in the middle of the inner side of the turnover cover 6 through the cleaning fixation, then clean the culture dish through the cleaning apparatus 5, and during the cleaning, the energy-saving mechanism arranged in the cleaning apparatus 5 can effectively clean the culture dish 28 for the first time through the recycled cleaning water, and discharge the cleaning water for the first time, and then filter and recycle the second time of the cleaning water through the unused cleaning water, which is the working principle of the culture apparatus for treating breast cancer and the stem cell cancer.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the invention.
Claims (7)
1. The utility model provides an adenocarcinoma stem cell culture apparatus for breast cancer immunotherapy, includes culture apparatus wholly (1), belt cleaning device (5) and upset lid (6), its characterized in that: the novel culture device is characterized in that a display screen (2) is embedded into the top of the right front left side of the culture device body (1), a control board (3) is embedded into the bottom of the right front left side of the culture device body (1), a suction door (4) is movably arranged at the middle top of the right front side of the culture device body (1), a cleaning device (5) is arranged at the right end of the culture device body (1), a turnover cover (6) is movably arranged at the top of the cleaning device (5), a water inlet pipe (7) is embedded into the top of the right side of the cleaning device (5), and a drain pipe (8) is embedded into the bottom of the right side of the cleaning device (5);
the novel culture device is characterized in that a first driving motor (9) is arranged on the left side of the top of the whole culture device (1), a liquid pump (12) is fixedly arranged in the middle of the top of the whole culture device (1), a hydraulic telescopic rod (13) is arranged at the bottom end of the liquid pump (12) in a communicating manner, a black cover (14) is fixedly arranged at the bottom of the hydraulic telescopic rod (13), a cleaning brush head (15) is movably arranged on the left side of the inside of the cleaning device (5), a second driving motor (17) is fixedly arranged at the bottom end of the cleaning brush head (15), a water spray head (18) is arranged on the right side of the inside of the cleaning device (5), a first three-way electromagnetic valve (19) is arranged at the bottom end of the water spray head (18), a conveying pipe (20) is arranged at the middle bottom of the first three-way electromagnetic valve (19) in a communicating manner, a conveying pump (22) is arranged at the bottom of the conveying pipe (20) in a communicating manner, a cleaning water recovery cavity (21) is arranged at the periphery of the conveying pump (22), and a second three-way electromagnetic valve (23) is arranged on the right side of the middle of the cleaning device (5);
a third driving motor (601) is arranged in the middle of the inside of the turnover cover (6), a limiting placing rotary plate (602) is arranged at the bottom end fixing of the third driving motor (601), an operation handle (603) is embedded and arranged at the right side of the bottom of the limiting placing rotary plate (602), a transmission hinge (604) is arranged at the periphery of the top of the operation handle (603) in a nested mode, a rotary threaded column (605) is arranged at the inner left side of the transmission hinge (604) in a linkage mode, a fixing column (606) is arranged at the middle position of the rotary threaded column (605) in a penetrating mode, a gear disc (607) is arranged at the bottom end fixing of the fixing column (606), a nested nut (608) is arranged at the periphery of the bottom of the rotary threaded column (605) in a nested mode, and an elastic support column (609) is fixedly arranged at the bottom of the nested nut (608);
a fourth driving motor (1401) is arranged in the middle of the inside of the black cover (14), a rolling disc (1402) is arranged right in front of the fourth driving motor (1401) in a linkage mode, traction ropes (1403) are fixedly arranged on two sides of the rolling disc (1402), an abutting spring group (1405) is fixedly arranged at the bottom end of each traction rope (1403), and a protruding block (1406) is fixedly arranged on the outer side of each abutting spring group (1405);
the water inlet pipe (7) is matched with the water outlet pipe (8), the water spraying head (18), the first three-way electromagnetic valve (19), the conveying pipe (20), the cleaning water recovery cavity (21), the conveying pump (22) and the second three-way electromagnetic valve (23) together to form an energy-saving mechanism;
the operating handle (603) is matched with the transmission hinge (604), the rotary threaded column (605), the fixed column (606), the gear disc (607), the nested nut (608) and the elastic support column (609) for use together to form a cleaning and fixing mechanism;
the black cover (14) is matched with a fourth driving motor (1401), a winding disc (1402), a traction rope (1403), an abutting spring group (1405) and a protruding block (1406) for use together to form an automatic cover taking mechanism.
2. An adenocarcinoma stem cell culture device for the immunotherapy of breast cancer according to claim 1, characterized in that: the periphery of the bottom of the first driving motor (9) is provided with a ring gear (10) in a linkage way.
3. An adenocarcinoma stem cell culture device for the immunotherapy of breast cancer according to claim 2, characterized in that: an ultraviolet sterilizing lamp (11) is arranged at the bottom of the left side of the ring gear (10).
4. An adenocarcinoma stem cell culture device for the immunotherapy of breast cancer according to claim 1, characterized in that: the inner side of the hauling rope (1403) is movably provided with a steering pulley block (1404).
5. An adenocarcinoma stem cell culture device for the immunotherapy of breast cancer according to claim 1, characterized in that: umbrella-shaped stop blocks (16) are fixedly arranged at the middle part of the top of the second driving motor (17).
6. An adenocarcinoma stem cell culture device for the immunotherapy of breast cancer according to claim 1, characterized in that: the filter element (24) is embedded in the middle of the right side of the cleaning device (5), and a filter element supporting seat (25) is fixedly arranged on the periphery of the bottom of the filter element (24).
7. An adenocarcinoma stem cell culture device for the immunotherapy of breast cancer according to claim 6, characterized in that: the inside of filter core supporting seat (25) is provided with support spring group (26), the middle outside of filter core supporting seat (25) is provided with gum cover (27).
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| CN202210038165.2A CN114381371B (en) | 2022-01-13 | 2022-01-13 | Adenocarcinoma stem cell culture device for breast cancer immunotherapy |
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| CN202210038165.2A CN114381371B (en) | 2022-01-13 | 2022-01-13 | Adenocarcinoma stem cell culture device for breast cancer immunotherapy |
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| CN114381371B true CN114381371B (en) | 2023-11-07 |
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| CN117683634B (en) * | 2024-01-31 | 2024-05-07 | 山东格林医学科技有限公司 | Stem cell culture device and method for diabetes treatment |
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