CN101701188A - Non-direct contact type cell co-culture device - Google Patents
Non-direct contact type cell co-culture device Download PDFInfo
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- CN101701188A CN101701188A CN200910209297A CN200910209297A CN101701188A CN 101701188 A CN101701188 A CN 101701188A CN 200910209297 A CN200910209297 A CN 200910209297A CN 200910209297 A CN200910209297 A CN 200910209297A CN 101701188 A CN101701188 A CN 101701188A
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Abstract
The invention relates to a non-direct contact type cell co-culture device which belongs to the technical field of cell co-culture devices. The non-direct contact type cell co-culture device consists of a culture hole, a culture plate and an upper cover, the culture hole is attached on the culture plate, and the upper cover is covered on the culture plate and seals the culture hole; and an isolation plate is arranged in the culture hole for dividing the culture hole into two or more culture small chambers. The device comprises one or more isolation plates, the isolation plate is integrated with the culture hole and the culture plate into a whole and can not be detached, the culture small chambers are provided with sequencing labels, micro-holes with the diameters of 0.5-1 microns are formed on the isolation plate, and the height of the isolation plate is 2/3 of that of the culture hole. When in use, the isolation plate can not only effectively play the isolation role, but also facilitate the experimental operation and reduce the pollution. The micro-holes can allow a variety of nutrients, macromolecular proteins and other cell products to pass through freely and further prevent cells from passing through. The non-direct contact type cell co-culture device has the advantages of simple operation, ability of realizing the co-culture of a variety of cells, low cost and reliable effect.
Description
Technical field
The present invention relates to a kind of non-direct contact type cell co-culture device, belong to the cell co-culture device technical field.
Background of invention:
Different cells are placed same environment co-cultivation, more and more be applied to medical research field by interacting and transform between the research cell.Yet cell co-culture device commonly used at present need be united the matrigel (Matrix gel) and the Transwell that use costliness and be cultivated cell, cultivates altogether to realize two kinds of intercellular non-direct contacts.Obviously there is following shortcoming in this culture apparatus: 1. cost an arm and a leg: matrigel (Matrix gel) and Transwell cultivate the high price of cell, have increased experimental cost greatly; 2. operation easier is big, the mortality height: matrigel has and just solidifies rapidly at normal temperatures and irreversible characteristic, makes complex operation, and easily causes colloid skewness in the operation, has increased the experimental implementation difficulty; 3. can not realize the common cultivation of two or more attached cells.Because the many disadvantages that existing cell co-culture device exists has limited the development of co-culture of cells research, therefore be badly in need of the cell co-culture device of a kind of more convenient practicality of research and development.
Summary of the invention:
At the deficiency of existing cell co-culture device, the invention provides a kind of reliable for effect, easy to use, with low cost and can be applicable to the non-direct contact type cell co-culture device that various kinds of cell is cultivated.
A kind of non-direct contact type cell co-culture device, form by culture hole, culture plate and loam cake, culture hole is attached on the culture plate, loam cake covers on the culture plate and seals culture hole, be provided with division board in the culture hole culture hole is divided into two or more cultivation cells, division board and culture hole, culture plate combine together, and be non-dismountable.
Described division board is provided with 1-4.
Described division board is provided with the micropore of diameter between the 0.5-1 micron.
The height of described division board is 2/3 of a culture hole height.
Using method:
In the use, division board can effectively play buffer action, is convenient to experimental implementation again, reduces the generation of polluting.Micropore freely passes through can allow cellular products such as various nutritive substances and macro-molecular protein, can prevent that again cell from passing through to be principle.Cell culture apparatus should guarantee the absolutesterility state before the Kaifeng.During use, the suspension that will contain different cells adds cultivation cell separately respectively, notices that liquid level must not surmount division board.According to concrete requirement of experiment, with different co-culture of cells after for some time, by cell growth state before and after cultivating relatively altogether, surface marker changes and nutrient solution becomes to grade, and index is studied different intercellular interactions.
Advantage: this non-direct contact type cell co-culture device has the following advantages:
1, simple to operate, same substantially single cell culture has been avoided complex operating steps in the past.
2, can realize that various kinds of cell cultivates altogether, having solved co-culture device can not be with a wide range of applications to the defective of two or more attached cell co-cultivation.
3, with low cost.Because do not need to cost an arm and a leg and the short matrigel of validity period, therefore greatly reduce experimental cost, be fit to promote the use of.
4, reliable for effect, compare with single cell culture, do not increase any possibility deleterious material of pair cell and device, do not influence the normal adherent of cell and propagation.
Description of drawings
Fig. 1 is a structural representation of the present invention.
Fig. 2 is the structural representation of a culture hole of the present invention.
Fig. 3 is the structural representation of the embodiment of the invention 2.
Fig. 4 is the structural representation of the embodiment of the invention 3.
Wherein, 1, culture plate, 2, culture hole, 3, loam cake, 4, cultivate hole wall, 5, division board, 6, micropore, 7, culture hole bottom surface.
Embodiment
Embodiment 1:
A kind of non-direct contact type cell co-culture device, as depicted in figs. 1 and 2, form by culture hole 2, culture plate 1 and loam cake 3, culture hole 2 is attached on the culture plate 1, loam cake 3 covers on the culture plate 1 and seals culture hole 2, be provided with division board 5 in the culture hole 2 culture hole is divided into 2 cultivation cells, division board 5 combines together with culture hole 2, culture plate 1, and is non-dismountable.Can realize the common cultivation of two kinds of different attached cells.
Described division board 5 is provided with the micropore 6 of diameter between the 0.5-1 micron.
The height of described division board 5 is 2/3 of culture hole 2 height.
Embodiment 2:
A kind of non-direct contact type cell co-culture device, structure is substantially the same manner as Example 1, and difference is: be provided with 3 division boards 5 in the culture hole 2, culture hole 2 be divided into 3 cultivate cell, can realize the common cultivation of three kinds of different attached cells, as shown in Figure 3.
Embodiment 3:
A kind of non-direct contact type cell co-culture device, structure is substantially the same manner as Example 1, and difference is: be provided with 4 division boards 5 in the culture hole 2, culture hole 2 be divided into 4 cultivate cell, can realize the common cultivation of four kinds of different attached cells, as shown in Figure 4.
Claims (4)
1. non-direct contact type cell co-culture device, form by culture hole, culture plate and loam cake, culture hole is attached on the culture plate, loam cake covers on the culture plate and seals culture hole, it is characterized in that, be provided with division board in the culture hole culture hole is divided into two or more cultivation cells, division board and culture hole, culture plate combine together, and be non-dismountable.
2. a kind of non-direct contact type cell co-culture device as claimed in claim 1 is characterized in that, described division board is provided with 1-4.
3. a kind of non-direct contact type cell co-culture device as claimed in claim 1 is characterized in that, described division board is provided with the micropore of diameter between the 0.5-1 micron.
4. a kind of non-direct contact type cell co-culture device as claimed in claim 1 is characterized in that, the height of described division board is 2/3 of a culture hole height.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910209297A CN101701188A (en) | 2009-10-29 | 2009-10-29 | Non-direct contact type cell co-culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910209297A CN101701188A (en) | 2009-10-29 | 2009-10-29 | Non-direct contact type cell co-culture device |
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| Publication Number | Publication Date |
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| CN101701188A true CN101701188A (en) | 2010-05-05 |
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| CN200910209297A Pending CN101701188A (en) | 2009-10-29 | 2009-10-29 | Non-direct contact type cell co-culture device |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103146577A (en) * | 2013-03-14 | 2013-06-12 | 泰山医学院 | Microporous grid plate separation type multi-tissue culture dish |
| CN103194391A (en) * | 2013-03-14 | 2013-07-10 | 泰山医学院 | Microporous grid plate isolated multi-tissue culture plate |
| CN103805513A (en) * | 2014-03-12 | 2014-05-21 | 山东大学 | Cell culture plate for cell immunofluorescence and immunochemistry research |
| CN105695328A (en) * | 2016-04-11 | 2016-06-22 | 张姝 | Co-culture device for cell culture and cell-cell interaction and using method of co-culture device |
| CN106479893A (en) * | 2016-10-31 | 2017-03-08 | 北京科技大学 | The device and method that one kind of multiple cells patterning co-cultures |
| CN117683634A (en) * | 2024-01-31 | 2024-03-12 | 山东格林医学科技有限公司 | Stem cell culture device and method for diabetes treatment |
-
2009
- 2009-10-29 CN CN200910209297A patent/CN101701188A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103146577A (en) * | 2013-03-14 | 2013-06-12 | 泰山医学院 | Microporous grid plate separation type multi-tissue culture dish |
| CN103194391A (en) * | 2013-03-14 | 2013-07-10 | 泰山医学院 | Microporous grid plate isolated multi-tissue culture plate |
| CN103805513A (en) * | 2014-03-12 | 2014-05-21 | 山东大学 | Cell culture plate for cell immunofluorescence and immunochemistry research |
| CN105695328A (en) * | 2016-04-11 | 2016-06-22 | 张姝 | Co-culture device for cell culture and cell-cell interaction and using method of co-culture device |
| CN106479893A (en) * | 2016-10-31 | 2017-03-08 | 北京科技大学 | The device and method that one kind of multiple cells patterning co-cultures |
| CN106479893B (en) * | 2016-10-31 | 2020-06-19 | 北京科技大学 | Device and method for patterning co-culture of multiple cells |
| CN117683634A (en) * | 2024-01-31 | 2024-03-12 | 山东格林医学科技有限公司 | Stem cell culture device and method for diabetes treatment |
| CN117683634B (en) * | 2024-01-31 | 2024-05-07 | 山东格林医学科技有限公司 | Stem cell culture device and method for diabetes treatment |
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Open date: 20100505 |