CN203683554U - Oocyte and embryo culture device - Google Patents

Oocyte and embryo culture device Download PDF

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Publication number
CN203683554U
CN203683554U CN201420078486.6U CN201420078486U CN203683554U CN 203683554 U CN203683554 U CN 203683554U CN 201420078486 U CN201420078486 U CN 201420078486U CN 203683554 U CN203683554 U CN 203683554U
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CN
China
Prior art keywords
culture
culture dish
base
embryo
oocyte
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Expired - Fee Related
Application number
CN201420078486.6U
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Chinese (zh)
Inventor
曹新燕
魏海军
赵伟刚
徐超
杨镒峰
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Institute Special Animal and Plant Sciences CAAS
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Institute Special Animal and Plant Sciences CAAS
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Priority to CN201420078486.6U priority Critical patent/CN203683554U/en
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Publication of CN203683554U publication Critical patent/CN203683554U/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

The utility model discloses an oocyte and embryo culture device and relates to a cell and embryo culture device. The problems of the failure of the experiment due to the mixing of different treatment droplets when the culture dish having large volume waggles and the cell contamination caused by the use of the remaining culture wells because the culture wells on the culture plates cannot be removable are solved. The oocyte and embryo culture device comprises a culture dish box and N culture dishes, wherein the culture dish box is a box having an upper opening, and the culture dishes are arranged in the inner cavity of the culture dish box. The oocyte and embryo culture device also comprises permanent magnet sheets and a culture dish base, the permanent magnet sheets are arranged on the outer surface of the bottom of each culture dish; the culture dish base comprises a base panel, a base bracket, a power supply, a base electromagnet and a power switch, the base panel is arranged at the upper surface of the base bracket, the base electromagnet is fixed at the bottom of the base panel, the base electromagnet is communicated with the power supply by virtue of the power switch and the culture dish box is arranged on the upper surface of the culture dish base. The oocyte and embryo culture device disclosed by the utility model is applied to the cell and embryo culture device.

Description

Ovocyte, embryo culture device
Technical field
The utility model relates to cell and embryo culture device.
Background technology
Along with the progress of Embryo engineering technology, embryo biotechnology is widely used in increasing field, and has promoted many multi-disciplinary development such as medical science, medicine, herding, genetically engineered, protection of animal.Vitro culture is the core of embryo biotechnology, and ovocyte and In vitro culture all need to use culture vessel, at present main culture dish and the culture plate of adopting.On culture plate, there are multiple culture hole, worry is generally 6,12,24,48,96 holes, in each batch of experiment, only uses a culture hole or a few culture hole, because can increase cell contamination probability, on culture plate, remaining culture hole is also seldom utilized again, causes experimental cost to increase.Existing culture dish, because volume is larger, is also unsuitable for the vitro culture of less cell, and whole culture dish all needs to cover paraffin oil, and not only wasting space is also wasted paraffin oil; If cultivate many group cells in a culture dish, can avoid waste, but close together between the cultivation drop in culture dish can cause different treatment droplets mixing because culture dish rocks, and causes the failure of an experiment.Therefore, a kind of ovocyte, the In vitro culture device that can certainly separate above-mentioned defect is badly in need of in embryo biotechnology field at present.
Utility model content
The purpose of this utility model is large in order to solve existing culture dish volume, cultivates a small amount of cell wasting space, waste experiment material, and the many groups of cultivation drops are scrupled again because culture dish rocks and caused different treatment droplets mixing to cause the failure of an experiment; Adopt culture plate to cultivate ovocyte and embryo, if use culture hole can add the problems such as the contaminated probability of maxicell in batches, and provide a kind of ovocyte, embryo culture device.
Ovocyte, embryo culture device, comprise a culture dish casing and N culture dish, N is greater than 1 natural number, culture dish casing is the casing of upper opening, N culture dish is placed in the inner chamber of culture dish casing, it also comprises permanent magnet pieces and culture dish base, and permanent magnet pieces is arranged on the outside surface of each culture dish bottom; Culture dish base comprises base panel, base support, power supply, base electro-magnet and power switch, base panel is arranged on the upper surface of base support, base electro-magnet is fixedly connected on the bottom of base panel, and base electro-magnet is communicated with power supply by power switch; Culture dish casing is placed in the upper surface of culture dish base.
In the utility model, base panel is irony base panel.
The utility model ovocyte, embryo culture device substitute existing capacious culture dish with the little culture dish of volume, save experiment usage space, save again the consumption of paraffin oil; And experiment is respectively placed in culture dish separately with cell, even if shake when mobile the utility model culture apparatus, also can not there is the situation of droplets mixing, ensure the success ratio of experiment.In the utility model ovocyte, embryo culture device, the bottom of culture dish is provided with permanent magnet pieces, can be fixing with base panel absorption; In the time that needs take out culture dish, press power switch, the energising of base electro-magnet has magnetic, the electromagnetism polarity of base electro-magnet is identical with the polarity of permanent magnet pieces bottom surface, thereby produce mutual repulsive force, culture dish is separated with base panel, for convenience detach and replacing.The number of culture dish requires to determine according to specific experiment and chooses, and has stopped cell contamination problem, saves again experiment material.The utlity model has simple in structure, easy to usely, be not only applicable to ovocyte and In vitro culture, be also applicable to other cell culture experiments.
In culture dish of the present utility model, permanent magnet pieces is by unified identical polar setting.
Brief description of the drawings
Fig. 1 is sectional view of the present utility model, and Fig. 2 is the sectional view of culture dish in embodiment one.
Embodiment
Embodiment one: present embodiment is described in conjunction with Fig. 1 and Fig. 2, ovocyte, embryo culture device described in present embodiment, it comprises culture dish casing 1 and N culture dish 2, N is greater than 1 natural number, the casing that culture dish casing 1 is upper opening, N culture dish 2 is placed in the inner chamber of culture dish casing 1, and it also comprises permanent magnet pieces 2-1 and culture dish base 3, and permanent magnet pieces 2-1 is arranged on the outside surface of each culture dish 2 bottoms; Culture dish base 3 comprises base panel 3-1, base support 3-2, power supply 3-3, base electro-magnet 3-4 and power switch 3-5, base panel 3-1 is arranged on the upper surface of base support 3-2, base electro-magnet 3-4 is fixedly connected on the bottom of base panel 3-1, and base electro-magnet 3-4 is communicated with power supply 3-3 by power switch 3-5; Culture dish casing 1 is placed in the upper surface of culture dish base 3.
The permanent magnet pieces 2-1 arranging on present embodiment ovocyte, embryo culture device culture dish 2 bottom outer surfaces is dismantled and assembled.
Embodiment two: in conjunction with Fig. 1, present embodiment is described, present embodiment is the further restriction to ovocyte, embryo culture device described in embodiment one, and base panel 3-1 adopts iron plate.
Embodiment three: in conjunction with Fig. 1, present embodiment is described, present embodiment is the further restriction to ovocyte, embryo culture device described in embodiment one, the iron core of base electro-magnet 3-4 adopts soft iron.
Embodiment four: in conjunction with Fig. 1, present embodiment is described, present embodiment is the further restriction to ovocyte, embryo culture device described in embodiment one, and the internal surface of culture dish casing 1 is provided with mark.
Set mark makes experimenter can easily remember the processing experiment of each culture dish 2.
Of the present utility model having particular application as:
N required culture dish 2 of experiment is placed in to the inner chamber of culture dish casing 1, culture dish casing 1 is placed on to the upper surface of culture dish base 3.
Because the volume of culture dish 2 is less than the volume of existing culture dish, in experiment, save the consumption of paraffin oil.
Because the bottom of culture dish 2 is provided with permanent magnet pieces 2-1, in the time that culture dish casing 1 is placed on the upper surface of culture dish base 3, culture dish 2, by the iron plate absorption of permanent magnet pieces 2-1 and base panel 3-1, keeps fixing; In experimentation, because base electro-magnet 3-4 no power does not produce magnetic, make the permanent magnet pieces 2-1 of culture dish 2 and base panel 3-1 keep adsorbed state.In the time that needs take out culture dish 2, press power switch 3-5, after base electro-magnet 3-4 energising, produce magnetic, make the electromagnetism polarity of base electro-magnet 3-4 identical with the polarity of permanent magnet pieces 2-1 bottom surface, thereby mutually produce repulsive force, culture dish 2 is separated with base panel 3-1.Solve culture hole or culture dish on existing culture plate non-dismountable, recycled the problem that residue culture hole causes cell contamination.
The utlity model has simple in structure, easy to usely, be not only applicable to ovocyte and In vitro culture, be also applicable to other cell culture experiments.

Claims (4)

1. ovocyte, embryo culture device, it comprises culture dish casing (1) and N culture dish (2), N is greater than 1 natural number, the casing that culture dish casing (1) is upper opening, N culture dish (2) is placed in the inner chamber of culture dish casing (1), it is characterized in that, it also comprises permanent magnet pieces (2-1) and culture dish base (3), and permanent magnet pieces (2-1) is arranged on the outside surface of each culture dish (2) bottom; Culture dish base (3) comprises base panel (3-1), base support (3-2), power supply (3-3), base electro-magnet (3-4) and power switch (3-5), base panel (3-1) is arranged on the upper surface of base support (3-2), base electro-magnet (3-4) is fixedly connected on the bottom of base panel (3-1), and base electro-magnet (3-4) is communicated with power supply (3-3) by power switch (3-5); Culture dish casing (1) is placed in the upper surface of culture dish base (3).
2. ovocyte, embryo culture device according to claim 1, is characterized in that, base panel (3-1) is irony base panel.
3. ovocyte, embryo culture device according to claim 1, is characterized in that, the iron core of base electro-magnet (3-4) adopts soft iron.
4. ovocyte, embryo culture device according to claim 1, is characterized in that, the internal surface of culture dish casing (1) is provided with mark.
CN201420078486.6U 2014-02-24 2014-02-24 Oocyte and embryo culture device Expired - Fee Related CN203683554U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201420078486.6U CN203683554U (en) 2014-02-24 2014-02-24 Oocyte and embryo culture device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201420078486.6U CN203683554U (en) 2014-02-24 2014-02-24 Oocyte and embryo culture device

Publications (1)

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CN203683554U true CN203683554U (en) 2014-07-02

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105838583A (en) * 2016-05-27 2016-08-10 中国科学院广州生物医药与健康研究院 Automatic shelf device for storing culture dishes and full-automatic cell culture device
CN106497787A (en) * 2017-01-06 2017-03-15 上海知楚仪器有限公司 A kind of free drawing and pulling type shaken cultivation case of electromagnetic type claspless
CN106834100A (en) * 2017-04-13 2017-06-13 明光现代农业科技合作推广服务中心 A kind of animal embryo splits ware

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105838583A (en) * 2016-05-27 2016-08-10 中国科学院广州生物医药与健康研究院 Automatic shelf device for storing culture dishes and full-automatic cell culture device
CN106497787A (en) * 2017-01-06 2017-03-15 上海知楚仪器有限公司 A kind of free drawing and pulling type shaken cultivation case of electromagnetic type claspless
CN106834100A (en) * 2017-04-13 2017-06-13 明光现代农业科技合作推广服务中心 A kind of animal embryo splits ware
CN106834100B (en) * 2017-04-13 2023-09-29 明光现代农业科技合作推广服务中心 Animal embryo segmentation dish

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C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20140702

Termination date: 20160224